CN112136526A - Greenhouse castanea henryi twig cutting seedling method - Google Patents
Greenhouse castanea henryi twig cutting seedling method Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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- A01N43/74—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
- A01N43/78—1,3-Thiazoles; Hydrogenated 1,3-thiazoles
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
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- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/36—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< directly attached to at least one heterocyclic ring; Thio analogues thereof
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Abstract
The invention provides a method for raising seedlings of castanea henryi by cutting tender branches in a greenhouse, relates to the technical field of propagation of castanea henryi seedlings, and aims to solve the problem of low rooting rate of the existing method for raising the tender branches of castanea henryi by cutting. The method comprises the following steps: before picking the ears, carrying out rejuvenation treatment on the ears and then preparing a cutting bed; collecting the young shoots of the current-year-old new shoots of the grafted seedlings of the excellent variety of the castanea henryi as cutting slips in 5 months, sterilizing the cutting slips by using carbendazim, soaking the sterilized cutting slips in a rooting agent mixed with IBA, ZT and TDZ for 3-5min, timely cutting the sterilized cutting slips after the treatment of the rooting agent, and placing the sterilized cutting slips in an artificial greenhouse. After cuttage, attention is paid to management, the temperature is kept at 23-25 ℃, the humidity is kept at 70-80%, and the photoperiod is kept at 16-18 hours. The invention can obtain a large amount of high-quality cutting seedlings, the cutting propagation method is simple and convenient, the matrix cost is low, the propagation speed is high, and the rooting rate can reach more than 95%. The invention can be applied to the field of rapid propagation of forest seedlings in Fagaceae.
Description
Technical Field
The invention relates to the technical field of production of quick propagation of castanea henryi seedlings in forest seedling raising, and particularly relates to a method for raising seedlings of castanea henryi by cutting tender branches in a greenhouse.
Background
Castanea henryi Rehd. et Wils is a plant of the genus Castanea (Castanea) of the family Fagaceae (Fagaceae). The fruits are rich in starch and are important woody grain tree species in the south of China. The castanea henryi is solid in material and resistant to water and moisture, and is a fast-growing high-quality economic tree species for sleepers, buildings, shipbuilding and furniture. In recent years, the castanea henryi rapidly develops in mountainous areas such as Hunan, Fujian, Zhejiang and the like, the planting area reaches 100 million hectares at present, tens of thousands of tons of nuts are produced annually, and the yield reaches billions of yuan. The chinquapin industry develops healthily and stably, and high-quality fine-breed nursery stocks cannot be separated.
At present, the main production of the improved variety seedlings of the castanea henryi is grafting and seedling propagation, but grafting is incompatible, the seedling period is long, and the cost of manpower and material resources is high; the seedling is sown and propagated, and the offspring has larger variability. The castanea henryi twig cutting is an efficient asexual propagation technology, can shorten the breeding time of castanea henryi, and can produce excellent castanea henryi cutting seedlings on a large scale for production and application while maintaining the excellent properties of parents. However, because the chinquapin branch is rich in substances such as tannin, polyphenol and the like, the chinquapin branch is a plant which is difficult to root, and researches show that the chinquapin twig has high water content and strong tissue meristematic capacity, wound parts are easy to generate callus, and the callus is difficult to root. Meanwhile, the age of the parent tree is closely related to the formation of adventitious roots of the cutting, and the higher the age of the parent tree, the higher the lignification degree is, the lower the water content and the nutrient content of the cutting are, and the rooting rate of the cutting is reduced. In the early detection of the' Castanea henryi cutting seedling research, the King Xiaohong et al (2012) utilizes the Castanea henryi twigs to carry out the cutting rooting rate of 89%, but does not carry out the rejuvenation treatment on the mother trees, the cutting propagation time is in Maries of spring, and the quantity of the collected Castanea henryi twigs is less at the moment, thus being not beneficial to the large-scale propagation. In the study on the formula of the cutting medium of the castanea henryi, peat and perlite are selected as mixed medium for cutting the castanea henryi by the Xiaocauyuan (2013), although the rooting rate is 93%, the cost of the selected cutting medium is higher, and the investment of the breeding cost of the castanea henryi is increased. Therefore, the method adopts the technology of cutting seedling of the chinquapin twigs, and can solve the problems of long seedling period, low rooting rate, high seedling cost and the like of the chinquapin seedlings.
Disclosure of Invention
Aiming at the technical problems in the prior art, one of the purposes of the invention is to provide a method for raising seedlings of green house castanea henryi twigs by cutting, which solves the technical problem of low rooting rate of the existing castanea henryi cutting propagation and can improve the rooting rate of the cutting twigs.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a method for raising seedlings of Castanea henryi twigs by cutting in a greenhouse comprises the following steps:
s1, cutting and rejuvenating: in the former winter of cutting seedling raising, the grafted seedlings of the 2-year-old excellent variety of the castanea henryi are heavily pruned, and young annual branches are obtained in the next year;
s2, collecting ear strips: selecting a current-year-old new shoot of a good variety grafted seedling of the castanea henryi for collection when the seedling is in a semi-lignified or lignified state in 5 months per year, keeping moisture after collection, bringing the seedling back to a cutting field, and cutting the seedling to prepare a cutting slip on the current day;
s3, cutting preparation: cutting the ear strips collected in the step S2 into cuttings with the length of 10-12cm, removing all blades at the base of the cuttings, reserving 1-3 blades at the upper part, cutting half of the blades at the upper part of the reserved blades, and beveling the lower end of the cuttings on a single side;
s4, preparing a cutting bed: putting a cuttage substrate into a flowerpot, wherein the thickness of the cuttage substrate is three quarters of the height of the flowerpot; the cutting medium is yellow core soil;
s5, illumination and temperature and humidity regulation: placing the cuttage bed of S4 into an artificial greenhouse for regulating and controlling illumination and temperature and humidity, wherein the photoperiod is kept at 16-18 hours, the air temperature is kept at 23-25 ℃, and the air humidity is 70-80%;
s6, cutting the cutting wood: soaking the cutting slips processed by the S3 by using 0.5% carbendazim, dipping the cutting slips for 3-5 minutes by using a cutting rooting agent, and cutting after dipping, wherein the cutting depth is 7-9cm, and the cutting density is preferably that the cutting slips are just intersected by the liquid level; wherein, the cuttage rooting agent combination is as follows: 1-3g/L IBA +1-1.5mg/L ZT +1-1.5mg/L TDZ;
s7, management after cuttage: and S6, bagging and moisturizing the flower pot after cutting, and fixing the flower pot by using a rubber band to finish the method for raising seedlings of the castanea henryi twigs by cutting.
In some embodiments, the source of the scion wood in S1 is rejuvenated current-year shoots obtained from 2-year-old seedlings.
In some embodiments, when the seedlings are in a semi-lignified state in 5 months and 10 days to 20 days per year, the current-year young shoots of the grafted seedlings of the fine variety of the castanea henryi are selected for collection, the moisture is kept after the collection, and cutting is carried out on the current day to prepare cutting slips when the seedlings are in a cutting place;
in some embodiments, the plug length in S3 is 12 cm.
In some embodiments, the photoperiod is maintained at S5 for 16 hours, with an air temperature of 25 ℃ and an ambient humidity of 70%.
In some embodiments, the cutting rooting agent combination in S6 is 2g/L IBA +1mg/L ZT +1mg/L TDZ.
In some embodiments, the cutting depth in S6 is 8 cm.
In some embodiments, in the S4, the pot size is 17 × 18cm, and 4-6 plants are planted in each pot in the S6; preferably, 5 plants are planted in each flowerpot.
In some embodiments, in S7, the cutting of S6 is immediately followed by covering the opening of the pot with a plastic bag for moisture preservation and fixing with a rubber band.
In some embodiments, the cutting rooting time in S7 is 35-42 days.
The second object of the present invention is to provide a Castanea henryi seedling cultivated by the method according to any of the above embodiments.
Compared with the prior art, the invention has the following beneficial effects:
1. the method provided by the invention is simple, convenient, rapid and effective, can be used for reproducing a large number of castanea henryi cutting seedlings in a short time, can be used for laying a good foundation for the culture of excellent clone plants in the future and laying a good foundation for the research of castanea henryi breeding in the future.
2. The method provided by the invention can lead the cutting of the castanea henryi to propagate fast, the efficiency is high, the cost is low, and the rooting rate reaches more than 95%.
3. The method for cultivating the Castanea henryi seedlings can quickly provide sufficient high-quality seedlings for the construction of Castanea henryi forests, and meanwhile, the efficient asexual propagation method provides a good foundation for the research and popularization of Castanea henryi seedling cultivation.
Drawings
FIG. 1 is a flow chart of the greenhouse castanea henryi twig cutting seedling raising process;
FIG. 2 shows a cutting made in example 1 of the present invention;
FIG. 3 shows the cutting density of each flowerpot in example 1 of the present invention;
FIG. 4 shows the cutting of example 1 of the present invention followed by bagging;
FIG. 5 shows the greenhouse cultivation of cutting seedlings of Castanea henryi in example 1 of the present invention;
FIG. 6 shows the seedlings of Castanea henryi rooted after survival of cutting in example 1 of the present invention.
Detailed Description
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, but rather should be construed as broadly as the present invention is capable of modification in various respects, all without departing from the spirit and scope of the present invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
The terms used in this application: the yellow core soil is core soil below soil layer, is red soil developed by limestone, has certain viscosity, and has pH value of 4-6.
Example 1
Referring to fig. 1, the method for raising seedlings of green house castanea henryi by cutting includes the following steps:
s1, cutting and rejuvenating: in the former winter of cutting seedling raising, the grafted seedlings of the fine variety of the 2-year-old castanea henryi are heavily pruned, and young annual branches are obtained in the next year;
s2, collecting ear strips: selecting current-year-old young shoots of the grafted seedlings of the fine variety of the castanea henryi for collection when the seedlings are in a semi-lignified state in 5/15 days each year, keeping moisture after collection, bringing the young shoots back to a cutting field, and cutting the young shoots to prepare cutting slips in the current day;
s3, cutting preparation: cutting the ear collected in S2 to obtain 12cm long ear, removing all leaves at the base of the cut, reserving 2 leaves at the upper part, cutting half of the leaves at the upper part of the reserved leaves, beveling the lower end of the cut, and making the cut as shown in FIG. 2;
s4, preparing a cutting bed: filling a cuttage substrate yellow core soil into a flowerpot, wherein the size of the flowerpot is 17 multiplied by 18cm, the thickness of the cuttage substrate is about three quarters of the height of the flowerpot, and the thickness of the specific cuttage substrate in the embodiment is 13 cm;
s5, illumination and temperature and humidity regulation: placing the cutting bed in the S4 in an artificial greenhouse for regulating and controlling illumination and temperature and humidity, wherein the photoperiod is kept for 16 hours, the air temperature is kept at 25 ℃, and the air humidity is 70%;
s6, soaking the cutting slips prepared in S3 by using 0.5% carbendazim, dipping the cutting slips soaked by using the carbendazim for 3-5 minutes by using a cutting rooting agent, and cutting after dipping, wherein the cutting depth is 7-9cm, as shown in figure 3, the cutting density is preferably that the leaf surfaces of the cutting slips are just intersected, and particularly, 4-6 plants per pot are preferably selected;
s7, management after cuttage: as shown in the figures 4-5, after S6 cuttage, bagging on a flowerpot for moisturizing, and fixing by a rubber band, namely completing the cuttage of the chinquapin twig.
35-42 days after the cutting by the S7, taking out the survival cutting seedlings which take root, and the survival cutting seedlings which take root are shown in figure 6, and the rooting rate of the cutting slips reaches 95.08% by statistics.
Comparative examples 1 to 2
In order to verify the influence of different months on the cutting rooting rate of the castanea henryi, in comparative examples 1-2, scion shoots are collected respectively at 15 days in 3 months and 15 days in 7 months for making cutting shoots, other seedling raising steps are the same as those in example 1, and after 35-42 days of cutting, the cutting shoot rooting results are shown in table 1.
TABLE 1 influence of different months on the rooting rate of cutting of Castanea henryi
| Example 1 | Comparative example 1 | Comparative example 2 | |
| Rooting rate | 95.08％ | 0 | 21.21％ |
As shown in table 1, the influence on the cutting rooting rate of the castanea henryi in different months is large, all cutting seedlings (cutting shoots) made of the scion collected in 3 months die, the rooting rate of the cutting seedlings made of the scion collected in 7 months is only 21.21%, and the average length of adventitious roots is 9.3 cm; the rooting rate of the cutting seedlings made of the scions collected in 5 months is more than 95%, and the average length of the adventitious roots is 11.0 cm.
Comparative examples 3 to 5
The method for raising seedlings of Castanea henryi twigs by cutting in comparative examples 3-5 has the same seedling raising steps as in example 1, and is different from example 1 in that the cutting matrix in a cutting bed is different from that in example 1, wherein the cutting matrix selected in comparative example 3 is vermiculite; the cutting medium selected in the comparative example 4 is peat and river sand, wherein the volume ratio of the peat to the river sand is 3: 1; the cutting medium selected in the comparative example 5 is peat and yellow core soil, wherein the volume ratio of the peat to the yellow core soil is 1: 1; after the cultivation by the method of the comparative examples 3-5, the rooting results of the cutting seedlings of the castanea henryi are shown in the table 2.
TABLE 2 influence of different media on the rooting rate of cutting of Castanea henryi
| Example 1 | Comparative example 3 | Comparative example 4 | Comparative example 5 | |
| Rooting rate | 95.08％ | 34.85％ | 58.33％ | 61.11％ |
As shown in Table 2, the yellow core soil is used as a cutting medium, the cutting rooting rate can reach more than 95%, the cutting rooting effect is good, meanwhile, the yellow core soil is used as the cutting medium, the price is cheaper than that of substrates such as peat and perlite, and the cutting cost is greatly reduced.
In conclusion, the young Castanea henryi twigs (namely semi-lignification) are subjected to cuttage in 5 months, the yellow core soil is used as a cuttage matrix of the Castanea henryi, the rooting rate of cuttage reaches over 95%, the price of the used yellow core soil is low, and the cuttage cost is greatly reduced.
In addition, the method provided by the invention has the advantages that the cutting seedlings of the castanea henryi are cultured in a greenhouse, the temperature, the humidity and the photoperiod of the cutting seedling environment can be strictly controlled, and the cutting seedlings of the castanea henryi can be more scientifically cultured.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A method for raising seedlings of castanea henryi by cutting in a greenhouse is characterized by comprising the following steps:
s1, cutting and rejuvenating: in the former winter of cutting seedling raising, the grafted seedlings of the 2-year-old excellent variety of the castanea henryi are heavily pruned, and young annual branches are obtained in the next year;
s2, collecting ear strips: selecting a current-year-old new shoot of a good variety grafted seedling of the castanea henryi for collection when the seedling is in a semi-lignified or lignified state in 5 months per year, keeping moisture after collection, bringing the seedling back to a cutting field, and cutting the seedling to prepare a cutting slip on the current day;
s3, cutting preparation: cutting the ear strips collected in the step S2 into cuttings with the length of 10-12cm, removing all blades at the base of the cuttings, reserving 1-3 blades at the upper part, cutting half of the blades at the upper part of the reserved blades, and beveling the lower end of the cuttings on a single side;
s4, preparing a cutting bed: putting a cuttage substrate into a flowerpot, wherein the thickness of the cuttage substrate is three quarters of the height of the flowerpot; the cutting medium is yellow core soil;
s5, illumination and temperature and humidity regulation: placing the cuttage bed of S4 into an artificial greenhouse for regulating and controlling illumination and temperature and humidity, wherein the photoperiod is kept at 16-18 hours, the air temperature is kept at 23-25 ℃, and the air humidity is 70-80%;
s6, soaking the cutting slips processed by the S3 by using 0.5% carbendazim, dipping the cutting slips for 3-5 minutes by using a cutting rooting agent, and cutting after dipping, wherein the cutting depth is 7-9cm, and the cutting density is preferably that the leaf surfaces of the cutting slips are just intersected; wherein, the cuttage rooting agent combination is as follows: 1-3g/LIBA +1-1.5mg/L ZT +1-1.5mg/L TDZ;
s7, management after cuttage: and S6, bagging and moisturizing the flower pot after cuttage, and fixing the flower pot by using a rubber band to finish the cuttage of the chinquapin twig.
2. The method for raising seedlings of green house castanea henryi twigs by cutting according to claim 1, wherein the scion wood source in S1 is a rejuvenated current-year branch obtained by pruning a 2-year-old seedling.
3. The method for raising seedlings of green house castanea henryi twigs by cutting according to claim 1, wherein the length of the cuttings in S3 is 12 cm.
4. The method for raising seedlings of green house castanea henryi twigs by cutting as claimed in claim 1, wherein in S5, the photoperiod is maintained for 16 hours, the air temperature is 25 ℃ and the environmental humidity is 70%.
5. The method for raising seedlings of Castanea henryi shoots by cutting in greenhouse according to claim 1, wherein the combination of the cutting rooting agent in S6 is 2g/LIBA +1mg/L ZT +1mg/L TDZ.
6. The method for cutting the young branches of the castanea henryi as claimed in claim 1, wherein the cutting depth in the S6 is 8 cm.
7. The method for raising seedlings of Castanea henryi shoots by cutting as claimed in claim 1, wherein in S4, the size of the flowerpot is 17 x 18cm, and 4-6 plants are cut in each flowerpot in S6.
8. The method for raising seedlings of green house castanea henryi twigs by cutting according to claim 1, wherein in S7, the cutting of S6 is immediately covered with a plastic bag on a pot mouth for moisture preservation and is fixed by a rubber band.
9. The method for raising seedlings of Castanea henryi shoots by cutting as claimed in claim 1, wherein the cutting rooting time in S7 is 35-42 days.
10. A Castanea henryi seedling is characterized by being cultivated by the greenhouse Castanea henryi twig cutting seedling method of any one of claims 1-9.
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| CN113141890A (en) * | 2021-03-03 | 2021-07-23 | 江西环境工程职业学院 | Method for promoting cutting rooting and survival of castanea mollissima tannin extract adult tree cutting slips |
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