CN113141890A - Method for promoting cutting rooting and survival of castanea mollissima tannin extract adult tree cutting slips - Google Patents
Method for promoting cutting rooting and survival of castanea mollissima tannin extract adult tree cutting slips Download PDFInfo
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- CN113141890A CN113141890A CN202110233392.6A CN202110233392A CN113141890A CN 113141890 A CN113141890 A CN 113141890A CN 202110233392 A CN202110233392 A CN 202110233392A CN 113141890 A CN113141890 A CN 113141890A
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- 238000005520 cutting process Methods 0.000 title claims abstract description 259
- 240000004957 Castanea mollissima Species 0.000 title claims abstract description 80
- 235000018244 Castanea mollissima Nutrition 0.000 title claims abstract description 79
- 239000000284 extract Substances 0.000 title claims abstract description 58
- 238000000034 method Methods 0.000 title claims abstract description 34
- 230000004083 survival effect Effects 0.000 title claims abstract description 21
- 230000001737 promoting effect Effects 0.000 title claims abstract description 18
- 229920001864 tannin Polymers 0.000 title claims description 28
- 235000018553 tannin Nutrition 0.000 title claims description 28
- 239000001648 tannin Substances 0.000 title claims description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 47
- 238000011282 treatment Methods 0.000 claims abstract description 41
- 206010020649 Hyperkeratosis Diseases 0.000 claims abstract description 19
- 230000003716 rejuvenation Effects 0.000 claims abstract description 17
- 239000011159 matrix material Substances 0.000 claims abstract description 13
- 238000013138 pruning Methods 0.000 claims abstract description 12
- 239000005556 hormone Substances 0.000 claims abstract description 6
- 229940088597 hormone Drugs 0.000 claims abstract description 6
- 239000000243 solution Substances 0.000 claims description 53
- 230000000249 desinfective effect Effects 0.000 claims description 25
- 238000005507 spraying Methods 0.000 claims description 23
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 20
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 20
- 239000006013 carbendazim Substances 0.000 claims description 20
- 239000002689 soil Substances 0.000 claims description 20
- 241000196881 Castanopsis Species 0.000 claims description 18
- 229930006000 Sucrose Natural products 0.000 claims description 17
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 16
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 16
- 239000007864 aqueous solution Substances 0.000 claims description 16
- 239000004202 carbamide Substances 0.000 claims description 16
- 239000005720 sucrose Substances 0.000 claims description 16
- 238000004140 cleaning Methods 0.000 claims description 15
- 238000002791 soaking Methods 0.000 claims description 15
- 241000196324 Embryophyta Species 0.000 claims description 13
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 11
- 235000006667 Aleurites moluccana Nutrition 0.000 claims description 9
- 230000000844 anti-bacterial effect Effects 0.000 claims description 6
- 239000003899 bactericide agent Substances 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 6
- 239000005747 Chlorothalonil Substances 0.000 claims description 5
- 239000005842 Thiophanate-methyl Substances 0.000 claims description 5
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 5
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- CRQQGFGUEAVUIL-UHFFFAOYSA-N chlorothalonil Chemical compound ClC1=C(Cl)C(C#N)=C(Cl)C(C#N)=C1Cl CRQQGFGUEAVUIL-UHFFFAOYSA-N 0.000 claims description 5
- 230000007613 environmental effect Effects 0.000 claims description 5
- 229910001710 laterite Inorganic materials 0.000 claims description 5
- 239000011504 laterite Substances 0.000 claims description 5
- 235000019691 monocalcium phosphate Nutrition 0.000 claims description 5
- 239000004033 plastic Substances 0.000 claims description 5
- 239000002985 plastic film Substances 0.000 claims description 5
- 229920006255 plastic film Polymers 0.000 claims description 5
- 235000010333 potassium nitrate Nutrition 0.000 claims description 5
- 239000004323 potassium nitrate Substances 0.000 claims description 5
- 239000000758 substrate Substances 0.000 claims description 5
- 239000002344 surface layer Substances 0.000 claims description 5
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical group COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 claims description 5
- 238000009966 trimming Methods 0.000 claims description 5
- 235000011609 Pinus massoniana Nutrition 0.000 claims description 4
- 241000018650 Pinus massoniana Species 0.000 claims description 4
- 230000004720 fertilization Effects 0.000 claims description 4
- 241000607479 Yersinia pestis Species 0.000 claims description 3
- 201000010099 disease Diseases 0.000 claims description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 3
- 241000238631 Hexapoda Species 0.000 claims description 2
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- 230000015572 biosynthetic process Effects 0.000 description 12
- 239000002609 medium Substances 0.000 description 11
- LRBQNJMCXXYXIU-QWKBTXIPSA-N gallotannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@H]2[C@@H]([C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-QWKBTXIPSA-N 0.000 description 6
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- 244000025797 Castanea pumila Species 0.000 description 1
- 235000007763 Castanea pumila Nutrition 0.000 description 1
- 241000219428 Fagaceae Species 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 241000422846 Sequoiadendron giganteum Species 0.000 description 1
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- 230000010496 root system development Effects 0.000 description 1
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- 125000000185 sucrose group Chemical group 0.000 description 1
- 239000003104 tissue culture media Substances 0.000 description 1
- 238000009333 weeding Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/10—Mycorrhiza; Mycorrhizal associations
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/22—Improving land use; Improving water use or availability; Controlling erosion
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/40—Afforestation or reforestation
Abstract
The invention discloses a method for promoting cutting rooting and survival of a castanea mollissima adult tree cutting shoot, which aims to solve the technical problems of low callus promotion and difficult rooting of the current castanea mollissima adult tree cutting shoot cutting seedling; the method comprises the following steps: superior tree selection → cutting shoot collection and postharvest rejuvenation treatment → cutting matrix preparation and disinfection → cutting shoot pretreatment and pruning → hormone treatment → temperature treatment and water control → mycorrhiza treatment → seedling stage management, thereby completing the cutting seedling culture of the castanea mollissima adult trees. The method takes the castanea mollissima extract adult tree cutting slips as materials, and utilizes natural superior tree seed resources and an asexual cuttage rapid propagation method to achieve the purposes of improving the cutting rooting rate of the castanea mollissima extract adult tree cutting slips, promoting a developed root system and improving the survival rate, and realize the rapid utilization of superior trees.
Description
Technical Field
The invention belongs to the technical field of plant propagation, particularly belongs to a method for cutting propagation of a castanea mollissima extract adult tree cutting shoot, and particularly relates to a method for promoting rooting and survival of the castanea mollissima extract adult tree cutting shoot cutting.
Background
The castanea mollissima tannin extract belongs to castanea plant of evergreen fagaceae with long and strip-shaped leaves, is a main colony-building species and a dominant species of top-level communities of evergreen broad-leaved forests in China, is an excellent greening and ecological tree species, and can become excellent tree species for materials and urban greening through genetic improvement; meanwhile, the chinquapin tannin extract is also an important resource plant of wood, nuts and tannin extract in the south of China, has wide application in economic and fruit dual-purpose, landscaping development and fire-proof forest belt construction, is familiar to vast forest farmers, has important economic and ecological values, and is mainly used for seeding and seedling culture in artificial forest production.
However, the problems of few seedlings for sowing and seedling raising, irregular emergence, poor seedling quality and the like are easily caused by the fact that the castanopsis mollissima is easy to damage by diseases and pests and a large amount of seeds are consumed for eating rodent because the castanopsis mollissima is long in fructification period and the seeds are susceptible to infectious microbes; on the other hand, the castanopsis mollissima seedlings have strong uprooting property, so that the problem of undeveloped root systems with long main roots and few lateral roots exists, the root forcing effect of the root cutting treatment of the sowed seedlings is not ideal, the afforestation survival rate after transplantation is low, for example, the afforestation survival rate of bare-rooted seedling transplantation in 1-2 years is as low as about 10 percent, the time of the seedling reviving period is long, even if the container seedlings are transplanted in 1 year, the instantaneity can be inhibited due to the inherent shortage of the root absorbing base number, and the management and protection cost of the seedling stage and the young stand is increased, so that the degree of good planting of the castanopsis mollissima artificial forest is low, and the planting scale is small. These problems affect the scale of the existing planting development of the castanea mollissima extract, and the economic development and the utilization value thereof are greatly limited.
At present, aiming at the root system problem of the seedling raising of the castanopsis mollissima extract, root cutting treatment is mainly carried out at the radicle germination stage or the seedling stage, so that the main root is inhibited, the lateral root growth is promoted, and a developed level absorption root system is expected to be cultivated, so as to be beneficial to the survival and the growth recovery of the castanopsis mollissima extract transplantation. Although the root cutting treatment can better cultivate developed root systems, when the root cutting is too early (such as the germination period of radicles), the inhibition effect of the main root is not ideal, and the purpose of cultivating the root systems cannot be achieved; if the root cutting is too late, the root cutting is easy to damage the root system too much in the second seedling stage of seedling transplantation, and the incidence rate of seedling infection, germ decay and death is increased.
The other major problem of the castanea mollissima tannin seedling raising is that seeds are susceptible to infectious microbes, when the castanea mollissima seeds are not pretreated or treated improperly, the seed coats are dark in color and luster, and are easy to infect the infectious microbes to cause late germination, so that the problems of less emergence of seedlings, irregular emergence of seedlings and poor seedling quality occur. Therefore, in order to ensure uniform emergence of seedlings and high quality of seedlings, pretreatment is mostly carried out by adopting a sterilization mode of endogenous chemical agents or a mode of combining disinfection with low-temperature storage. However, the sterilization effect of the treatment modes is not ideal in practical use, not only is the production cost increased, but also the balance of endophytes in the seeds is damaged, and the problem of seedling emergence irregularity is not fundamentally relieved. For example, when the castanea mollissima tannin seeds are subjected to aseptic treatment, the germination is promoted in the tissue culture medium environment, the infectious rate of mixed bacteria still occurs by 90%, the emergence time is prolonged by more than 1 month, and the emergence rate is as low as 5%.
Nowadays, good variety clone seedling has excellent characteristics of keeping parents, so the good variety clone seedling is widely applied, however, although the cutting slips of the castanopsis mollissima adult big tree are rich, the branches are easy to age, the mildew rate of the cutting callus is high, the callus formation is low, the rooting quantity is low, the root system development is weak, and the survival rate is extremely low, namely the clone seedling has the problems of bad callus formation, low rooting rate and low survival rate, and the problem seriously restricts the cultivation of the castanopsis mollissima, so the corresponding research and development are urgently needed, and the seedling cultivation problem is expected to be solved.
Disclosure of Invention
(1) Technical problem to be solved
Aiming at the defects of the prior art, the invention aims to provide a method for promoting the cutting rooting and survival of the cutting slips of the castanopsis mollissima adult trees, which aims to solve the technical problems of low callus promotion and difficult rooting of the cutting slips of the current castanopsis mollissima adult trees.
(2) Technical scheme
In order to solve the technical problems, the invention provides a method for promoting the cutting rooting and survival of the cutting slips of the castanea mollissima extract adult trees, which comprises the following specific steps:
step one, selecting a superior tree; selecting healthy and strong Castanea mollissima superior tannin tree plants with straight trunks, small branch angles, round and uniform crown widths, and 15-40a tree ages and without quarantine diseases and insect pests;
step two, cutting collection and postharvest rejuvenation treatment; collecting newly-extracted branches facing southeast at the periphery of the crown of the castanea mollissima quel excellent tree in the last ten days of 3 months, taking the newly-extracted branches as cutting slips, firstly disinfecting the newly-extracted branches by using a carbendazim solution with the mass concentration of 0.5% for more than 24 hours, then cleaning the newly-extracted branches by using clean water, and carrying out rejuvenation treatment in an indoor shading dark environment;
step three, preparing and disinfecting a cutting medium; uniformly mixing laterite, turfy soil and calcium superphosphate according to the volume ratio of 60:30:2 to obtain a cutting matrix, spraying and disinfecting with a carbendazim solution with the mass concentration of 0.8%, piling into a conical pile, and covering and disinfecting with a plastic film for more than 24 hours to obtain a completely sterilized cutting matrix;
step four, pre-treating and trimming the cutting shoots; cutting the immature cutting slips in the girdling cut step two, cleaning the cutting slips by using clean water in a dark environment, soaking the cutting slips for more than 1h, and then disinfecting the cutting slips by using a carbendazim solution with the mass concentration of 0.5% for more than 24 h; after the pruning tool is disinfected, pruning the cutting slips in a normally-illuminated indoor environment, controlling the length of the pruned cutting slips to be 6-10cm and the thickness to be 0.4-1.6cm, reserving more than 2 bud positions, performing flat cutting on the upper gaps and performing oblique cutting on the lower gaps, and reserving 1-2 blades on the upper parts of the cutting slips to obtain the cutting slips;
in the step, the pruning and cutting are controlled to keep more than 2 bud positions, and the length of the internode distance is moderate and uniform; keeping 1-2 leaves at the upper part of the cutting, if the number is 2, taking the leaves which are symmetrical to two sides of the cutting, and cutting half leaves respectively.
Step five, hormone treatment; soaking the cutting slips obtained in the fourth step in an ABT rooting agent with the concentration of 300ppm for more than 10min, taking out the cutting slips, cleaning the cutting slips with clear water, and placing the cutting slips in KNO with the mass concentration of 0.03 percent3Soaking in water solution for more than 3 h; cutting the seedlings in a cutting medium of a cutting pool at the bottom of 3 months, watering thoroughly, building an arched transparent film with the concentration of 30-40%, and watering water every 5-7 days to keep the cutting seedbed moist;
in this step, the cutting is carried out using conventional methods.
Step six, temperature treatment and moisture control; spraying a bactericide every 10 days after cuttage so as to prevent the base of the cutting shoot from rotting, controlling the temperature of a cuttage seedbed to be 18-25 ℃ and the humidity to be 40-65%, and spraying urea and a potassium dihydrogen phosphate solution with the mass concentration of 0.03% every 10 days after the callus is piled up like a ball;
in the step, callus formation can be observed about 15 days after cutting, urea and potassium dihydrogen phosphate solution with the mass concentration of 0.03% are sprayed every 10 days after the callus is in a bulk accumulation state, the root formation of the cutting slips is promoted, and rooting can be seen about 40 days later.
Step seven, mycorrhiza treatment; in the period of starting roots of the Castanea mollissima extract cutting seedlings, spraying the bactericide is stopped, rhizosphere soil of the Castanea mollissima extract superior tree plants in the step I or clean soil on the surface layers of the Chinese chestnut forest and masson pine forest land is collected and covered on the bases of the Castanea mollissima extract cutting seedlings, the thickness of the roots is controlled to be 2-3cm, the environmental temperature of a cutting substrate is 20-27 ℃, and the humidity is 50-70% until the root systems of the Castanea mollissima extract develop in a net shape;
in the step, rhizosphere soil of the Castanea mollissima extract superior tree plant in the step one or clean soil on the surface layers of the Chinese chestnut forest and the masson pine forest are covered on the base part of the Castanea mollissima extract cutting seedling, and the thickness and the temperature and the humidity of a cutting substrate are controlled, so that ectogenic mycorrhiza of the Castanea mollissima extract can be bred, and developed clonal root systems are promoted.
Step eight, seedling management; transplanting the castanea mollissima cutting seedlings at the beginning of 7 months, controlling the distance between the seedlings to be 25-35cm, starting to perform additional fertilization 30 days after the transplanting, wherein the additional fertilization is performed for 2 times, urea solution with the mass concentration of 0.5% is applied for the first time in 8 months, and urea solution with the mass concentration of 0.5% and potassium nitrate solution with the mass concentration of 0.3% are applied for the second time in 9 months, so that the cutting seedling raising of the castanea mollissima adult trees is completed.
In this step, irrigation and weeding are also required at appropriate time.
The method of the invention takes the castanea mollissima extract adult tree cutting slips as materials, and utilizes natural superior tree fine variety resources and the asexual cutting rapid propagation method to achieve the purposes of improving the cutting rooting rate of the castanea mollissima extract adult tree cutting slips, promoting developed root systems and improving the survival rate, and realizing the rapid utilization of superior trees.
Preferably, in step one, the plant of the Castanea mollissima Castanea extract superior in ecological environment is selected from natural inferior Castanea mollissima extract broad-leaved mixed forest.
Preferably, in the second step, the specific process of the rejuvenation treatment is as follows: wrapping the cutting slips with black plastic bags, and reserving a space with the empty margin length of 5cm in a wrapping object; girdling is carried out at the joint of the next-year old branch and the new branch of this year, the girdling width is 0.2-0.5cm, the girdling depth reaches the phloem of the cutting, and the girdling opening is exposed outside the package; thirdly, placing the cutting slips into a sucrose aqueous solution with the mass concentration of 1% for water culture, and enabling the annular peeling openings to expose the water surface to be larger than 1 cm; during water culture, ensuring that a girdling opening is kept clean, if mildew is found, immediately removing the girdling opening, replacing a sucrose aqueous solution with the mass concentration of 1% at intervals of 3-5 days, performing water culture for 15-20 days, and obtaining young cuttings when the color of all the castanopsis mollissima tannin extract leaves in the package is changed into yellow;
in the step, girdling is carried out at the junction of the next-year old branch and the new branch of the current year, and the position is the position where the skin color of the stem is converted from yellow green to green; the water culture solution for water culture is sucrose aqueous solution with mass concentration of 1%, and a little KNO can be added into the sucrose aqueous solution3Thereby preventing the cutting from mildewing.
Preferably, in the sixth step, the bactericide is a 0.5% carbendazim or chlorothalonil solution.
Preferably, in the step eight, the castanea mollissima tannin extract cutting seedlings are transplanted, and the distance between the seedlings is controlled to be 30 cm.
Preferably, in the eighth step, thiophanate methyl is sprayed during the second topdressing, so as to prevent and treat the stem rot.
(3) Advantageous effects
Compared with the prior art, the invention has the beneficial effects that:
1. the method of the invention utilizes the optimal treatment of the Castanea mollissima extract adult tree cutting and the control of the callus to promote the environmental conditions, including the optimal tree selection, the cutting shading optimal treatment, the temperature control and the water treatment of the callus formation, thereby greatly promoting the callus formation and the subsequent rooting.
2. According to the method, the rejuvenation treatment, callus promotion, rooting cultivation and mycorrhiza cultivation of the cutting slips and the promotion combination of the rooting environment and the clonal root system of the mycorrhiza matrix are respectively carried out according to the process of cutting rooting, which is greatly different from the prior art that developed root systems are obtained by cutting roots with mechanical damage to the root systems, the damage to young root systems is greatly reduced, the effect of promoting developed root systems is achieved, and the survival rate of the clonal cutting seedlings and the quality of seedlings are improved.
Generally speaking, the method takes the castanea mollissima extract adult tree cutting slips as materials, and achieves the purposes of improving the cutting rooting rate of the castanea mollissima extract adult tree cutting slips, promoting developed root systems and improving the survival rate by utilizing natural superior tree seed resources and the asexual cutting rapid propagation method, thereby realizing the rapid utilization of superior trees.
Detailed Description
In order to make the technical means, the original characteristics, the achieved purposes and the effects of the invention easily understood and obvious, the technical solutions in the embodiments of the present invention are clearly and completely described below to further illustrate the invention, and obviously, the described embodiments are only a part of the embodiments of the present invention, but not all the embodiments.
Example 1
The specific embodiment case is a cutting method of the cutting slips of the castanea mollissima tannin extract adult trees, and the process is as follows:
step one, selecting a superior tree; selecting strong and quarantine pest-free excellent Chinese chestnut tannin tree plants with straight trunks, small branch angles, full and uniform crown widths and 15-40a tree ages in natural inferior Chinese chestnut tannin and broad-leaf mixed forests with excellent ecological environment;
step two, cutting collection and postharvest rejuvenation treatment; collecting newly-extracted branches facing southeast at the periphery of the crown of the castanea mollissima quel excellent tree in the last ten days of 3 months, taking the newly-extracted branches as cutting slips, firstly disinfecting the newly-extracted branches by using a carbendazim solution with the mass concentration of 0.5% for more than 24 hours, then cleaning the newly-extracted branches by using clean water, and carrying out rejuvenation treatment in an indoor shading dark environment;
the specific process of the rejuvenation treatment comprises the following steps: wrapping the cutting slips with black plastic bags, and reserving a space with the empty margin length of 5cm in a wrapping object; performing girdling at the joint of the next-year old branch and the current-year new branch (the position is the position where the bark color of the stem is converted from yellow green to green), wherein the girdling width is 0.2-0.3cm, the girdling depth reaches the phloem of the cutting shoot, and the girdling opening is exposed and wrapped; thirdly, placing the cutting slips into 1 percent of sucrose aqueous solution (a little KNO can be added into the sucrose aqueous solution)3So as to prevent the cutting from mildewing) and the exposed water surface of the girdling opening is more than 1 cm; during the water culture period, ensuring that the girdling opening is kept clean, if mildewing is found, immediately removing the girdling opening, and replacing the sucrose aqueous solution with 1 percent of mass concentration (in which the sucrose aqueous solution can also be used) which is prepared in situ every 3 to 4 daysBy adding a small amount of KNO3So as to prevent the cutting from mildewing), water culture is carried out for 15-16 days, and when the color of all the castanopsis mollissima tannin extract leaves in the package is changed into yellow, the young cutting is obtained.
Step three, preparing and disinfecting a cutting medium; uniformly mixing laterite, turfy soil and calcium superphosphate according to the volume ratio of 60:30:2 to obtain a cutting matrix, spraying and disinfecting with a carbendazim solution with the mass concentration of 0.8%, piling into a conical pile, and covering and disinfecting with a plastic film for more than 24 hours to obtain a completely sterilized cutting matrix;
step four, pre-treating and trimming the cutting shoots; cutting the immature cutting slips in the girdling cut step two, cleaning the cutting slips by using clean water in a dark environment, soaking the cutting slips for more than 1h, and then disinfecting the cutting slips by using a carbendazim solution with the mass concentration of 0.5% for more than 24 h; after the pruning tool is disinfected, pruning the cutting slips in a normally-illuminated indoor environment, controlling the length of the pruned cutting slips to be 6-8cm and the thickness to be 0.4-0.8cm, reserving more than 2 bud positions, keeping the lengths of the intervals of the bud positions to be medium and uniform, flatly cutting the upper gaps of the bud positions, obliquely cutting the lower gaps of the bud positions, and reserving 1-2 blades on the upper parts of the cutting slips to obtain the cutting slips;
step five, hormone treatment; soaking the cutting slips obtained in the fourth step in an ABT rooting agent with the concentration of 300ppm for more than 10min, taking out the cutting slips, cleaning the cutting slips with clear water, and placing the cutting slips in KNO with the mass concentration of 0.03 percent3Soaking in water solution for more than 3 h; cutting the seedlings in a cutting medium of a cutting pool at the bottom of 3 months, watering thoroughly, building an arched transparent film with the concentration of 30-40%, and watering water every 5-6 days to keep the cutting seedbed moist;
step six, temperature treatment and moisture control; spraying chlorothalonil solution with the mass concentration of 0.5% every 10 days after cuttage so as to prevent and control the base of the cutting shoot from rotting, controlling the temperature of a cuttage seedbed to be 18-25 ℃ and the humidity to be 40-65%, observing callus formation after about 15 days after cuttage, spraying urea and potassium dihydrogen phosphate solution with the mass concentration of 0.03% every 10 days after the callus forms a massive accumulation shape so as to promote the root formation of the cutting shoot, and observing rooting after about 40 days;
step seven, mycorrhiza treatment; in the period of starting roots of the Castanea mollissima tannin extract cutting seedlings, stopping spraying the carbendazim solution, collecting rhizosphere soil of the Castanea mollissima tannin extract superior tree plants in the step I, covering the rhizosphere soil on the bases of the Castanea mollissima tannin extract cutting seedlings, controlling the thickness of the rhizosphere soil to be 2-3cm, and enabling the ambient temperature of a cutting matrix to be 20-27 ℃ and the humidity to be 50-70% until the root networks of the Castanea mollissima tannin extracts grow;
step eight, seedling management; transplanting the castanea mollissima cutting seedlings at the beginning of 7 months, controlling the distance between seedlings to be 25cm, starting topdressing 30 days after transplanting, and carrying out topdressing for 2 times, wherein the first time is to pour a urea solution with the mass concentration of 0.5% at the beginning of 8 months, the second time is to pour a urea solution with the mass concentration of 0.5% and a potassium nitrate solution with the mass concentration of 0.3% at the beginning of 9 months, and spraying thiophanate methyl (so as to prevent and control stem rot) during the second topdressing, thereby completing the cutting seedling culture of the castanea mollissima adult trees.
Example 2
The specific embodiment case is a cutting method of the cutting slips of the castanea mollissima tannin extract adult trees, and the process is as follows:
step one, selecting a superior tree; selecting strong and quarantine pest-free excellent Chinese chestnut tannin tree plants with straight trunks, small branch angles, full and uniform crown widths and 15-40a tree ages in natural inferior Chinese chestnut tannin and broad-leaf mixed forests with excellent ecological environment;
step two, cutting collection and postharvest rejuvenation treatment; collecting newly-extracted branches facing southeast at the periphery of the crown of the castanea mollissima quel excellent tree in the last ten days of 3 months, taking the newly-extracted branches as cutting slips, firstly disinfecting the newly-extracted branches by using a carbendazim solution with the mass concentration of 0.5% for more than 24 hours, then cleaning the newly-extracted branches by using clean water, and carrying out rejuvenation treatment in an indoor shading dark environment;
the specific process of the rejuvenation treatment comprises the following steps: wrapping the cutting slips with black plastic bags, and reserving a space with the empty margin length of 5cm in a wrapping object; performing girdling at the joint of the next-year old branch and the current-year new branch (the position is the position where the bark color of the stem is converted from yellow green to green), wherein the girdling width is 0.3-0.4cm, the girdling depth reaches the phloem of the cutting shoot, and the girdling opening is exposed and wrapped; thirdly, placing the cutting slips into 1 percent of cane sugar water by mass concentrationThe solution (KNO can be added into the solution slightly3So as to prevent the cutting from mildewing) and the exposed water surface of the girdling opening is more than 1 cm; during the water culture, ensuring that the girdling opening is kept clean, if mildewing is found, immediately removing the girdling opening, and replacing the sucrose aqueous solution with the mass concentration of 1 percent which is prepared at present every 3 to 4 days (wherein a little KNO can be added into the sucrose aqueous solution)3So as to prevent the cutting from mildewing), water culture is carried out for 16-18 days, and when the color of all the castanopsis mollissima tannin extract leaves in the package is changed into yellow, the young cutting is obtained.
Step three, preparing and disinfecting a cutting medium; uniformly mixing laterite, turfy soil and calcium superphosphate according to the volume ratio of 60:30:2 to obtain a cutting matrix, spraying and disinfecting with a carbendazim solution with the mass concentration of 0.8%, piling into a conical pile, and covering and disinfecting with a plastic film for more than 24 hours to obtain a completely sterilized cutting matrix;
step four, pre-treating and trimming the cutting shoots; cutting the immature cutting slips in the girdling cut step two, cleaning the cutting slips by using clean water in a dark environment, soaking the cutting slips for more than 1h, and then disinfecting the cutting slips by using a carbendazim solution with the mass concentration of 0.5% for more than 24 h; after the pruning tool is disinfected, pruning the cutting slips in a normally-illuminated indoor environment, controlling the length of the pruned cutting slips to be 8-10cm and the thickness to be 0.8-1.2cm, reserving more than 2 bud positions, keeping the lengths of the intervals of the bud positions to be medium and uniform, flatly cutting the upper gaps of the bud positions, obliquely cutting the lower gaps of the bud positions, and reserving 1-2 blades on the upper parts of the cutting slips to obtain the cutting slips;
step five, hormone treatment; soaking the cutting slips obtained in the fourth step in an ABT rooting agent with the concentration of 300ppm for more than 10min, taking out the cutting slips, cleaning the cutting slips with clear water, and placing the cutting slips in KNO with the mass concentration of 0.03 percent3Soaking in water solution for more than 3 h; cutting the seedlings in a cutting medium of a cutting pool at the bottom of 3 months, watering thoroughly, building an arched transparent film with the concentration of 30-40%, and watering water every 5-6 days to keep the cutting seedbed moist;
step six, temperature treatment and moisture control; spraying chlorothalonil solution with the mass concentration of 0.5% every 10 days after cuttage so as to prevent and control the base of the cutting shoot from rotting, controlling the temperature of a cuttage seedbed to be 18-25 ℃ and the humidity to be 40-65%, observing callus formation after about 15 days after cuttage, spraying urea and potassium dihydrogen phosphate solution with the mass concentration of 0.03% every 10 days after the callus forms a massive accumulation shape so as to promote the root formation of the cutting shoot, and observing rooting after about 40 days;
step seven, mycorrhiza treatment; in the period of starting roots of the castanea mollissima tannin extract cutting seedlings, stopping spraying the chlorothalonil solution, collecting clean soil on the surface layer of the forest land of the castanea mollissima forest, covering the clean soil on the base parts of the castanea mollissima tannin extract cutting seedlings, controlling the thickness of the clean soil to be 2-3cm, and enabling the environmental temperature of a cutting substrate to be 20-27 ℃ and the humidity to be 50-70% until the root systems of the castanea mollissima tannin extracts develop in a net shape;
step eight, seedling management; transplanting the castanea mollissima cutting seedlings at the beginning of 7 months, controlling the distance between seedlings to be 30cm, starting topdressing 30 days after transplanting, and carrying out topdressing for 2 times, wherein the first time is to pour a urea solution with the mass concentration of 0.5% at the beginning of 8 months, the second time is to pour a urea solution with the mass concentration of 0.5% and a potassium nitrate solution with the mass concentration of 0.3% at the beginning of 9 months, and spraying thiophanate methyl (so as to prevent and control stem rot) during the second topdressing, thereby completing the cutting seedling culture of the castanea mollissima adult trees.
Example 3
The specific embodiment case is a cutting method of the cutting slips of the castanea mollissima tannin extract adult trees, and the process is as follows:
step one, selecting a superior tree; selecting strong and quarantine pest-free excellent Chinese chestnut tannin tree plants with straight trunks, small branch angles, full and uniform crown widths and 15-40a tree ages in natural inferior Chinese chestnut tannin and broad-leaf mixed forests with excellent ecological environment;
step two, cutting collection and postharvest rejuvenation treatment; collecting newly-extracted branches facing southeast at the periphery of the crown of the castanea mollissima quel excellent tree in the last ten days of 3 months, taking the newly-extracted branches as cutting slips, firstly disinfecting the newly-extracted branches by using a carbendazim solution with the mass concentration of 0.5% for more than 24 hours, then cleaning the newly-extracted branches by using clean water, and carrying out rejuvenation treatment in an indoor shading dark environment;
the specific process of the rejuvenation treatment comprises the following steps: wrapping the cutting slips with black plastic bagsReserving a space with the length of the vacant space of 5cm in the object; performing girdling at the joint of the next-year old branch and the current-year new branch (the position is the position where the bark color of the stem is converted from yellow green to green), wherein the girdling width is 0.4-0.5cm, the girdling depth reaches the phloem of the cutting shoot, and the girdling opening is exposed and wrapped; thirdly, placing the cutting slips into 1 percent of sucrose aqueous solution (a little KNO can be added into the sucrose aqueous solution)3So as to prevent the cutting from mildewing) and the exposed water surface of the girdling opening is more than 1 cm; during the water culture period, ensuring that the girdling opening is kept clean, if mildewing is found, immediately removing the girdling opening, and replacing the sucrose aqueous solution with the mass concentration of 1 percent which is prepared in situ every 4 to 5 days (wherein a little KNO can be added into the sucrose aqueous solution)3So as to prevent the cutting from mildewing), water culture is carried out for 18-20 days, and when the color of all the castanopsis mollissima tannin extract leaves in the package is changed into yellow, the young cutting is obtained.
Step three, preparing and disinfecting a cutting medium; uniformly mixing laterite, turfy soil and calcium superphosphate according to the volume ratio of 60:30:2 to obtain a cutting matrix, spraying and disinfecting with a carbendazim solution with the mass concentration of 0.8%, piling into a conical pile, and covering and disinfecting with a plastic film for more than 24 hours to obtain a completely sterilized cutting matrix;
step four, pre-treating and trimming the cutting shoots; cutting the immature cutting slips in the girdling cut step two, cleaning the cutting slips by using clean water in a dark environment, soaking the cutting slips for more than 1h, and then disinfecting the cutting slips by using a carbendazim solution with the mass concentration of 0.5% for more than 24 h; after the pruning tool is disinfected, pruning the cutting slips in a normally-illuminated indoor environment, controlling the length of the pruned cutting slips to be 7-9cm and the thickness to be 1.2-1.6cm, reserving more than 2 bud positions, keeping the lengths of the intervals of the bud positions to be medium and uniform, flatly cutting the upper gaps of the bud positions, obliquely cutting the lower gaps of the bud positions, and reserving 1-2 blades on the upper parts of the cutting slips to obtain the cutting slips;
step five, hormone treatment; soaking the cutting slips obtained in the fourth step in an ABT rooting agent with the concentration of 300ppm for more than 10min, taking out the cutting slips, cleaning the cutting slips with clear water, and placing the cutting slips in KNO with the mass concentration of 0.03 percent3Soaking in water solution for 3 hrThe above; cutting the seedlings in a cutting medium of a cutting pool at the bottom of 3 months, watering thoroughly, building an arched transparent film with the concentration of 30-40%, and watering water every 6-7 days to keep the cutting seedbed moist;
step six, temperature treatment and moisture control; spraying a carbendazim solution with the mass concentration of 0.5% every 10 days after cuttage so as to prevent and control the base of the cutting shoot from rotting, controlling the temperature of a cuttage seedbed to be 18-25 ℃ and the humidity to be 40-65%, observing callus formation after about 15 days after cuttage, spraying urea and a potassium dihydrogen phosphate solution with the mass concentration of 0.03% every 10 days after the callus forms a bulk accumulation shape, promoting the root formation of the cutting shoot, and enabling the root to be visible after about 40 days;
step seven, mycorrhiza treatment; in the period of starting roots of the castanopsis mollissima tannin extract cutting seedlings, stopping spraying the carbendazim solution, collecting clean soil on the surface layer of the masson pine forest land, covering the clean soil on the base parts of the castanopsis mollissima tannin extract cutting seedlings, controlling the thickness of the clean soil to be 2-3cm, and enabling the environmental temperature of a cutting substrate to be 20-27 ℃ and the humidity to be 50-70% until the net-shaped development of the roots of the castanopsis mollissima tannin extracts is realized;
step eight, seedling management; transplanting the castanea mollissima cutting seedlings at the beginning of 7 months, controlling the distance between seedlings to be 35cm, starting topdressing 30 days after transplanting, and carrying out topdressing for 2 times, wherein the first time is to pour a urea solution with the mass concentration of 0.5% at the beginning of 8 months, the second time is to pour a urea solution with the mass concentration of 0.5% and a potassium nitrate solution with the mass concentration of 0.3% at the beginning of 9 months, and spraying thiophanate methyl (so as to prevent and control stem rot) during the second topdressing, thereby completing the cutting seedling culture of the castanea mollissima adult trees.
Having thus described the principal technical features and basic principles of the invention, and the advantages associated therewith, it will be apparent to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, but is capable of other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description is described in terms of various embodiments, not every embodiment includes only a single embodiment, and such descriptions are provided for clarity only, and those skilled in the art will recognize that the embodiments described herein can be combined as a whole to form other embodiments as would be understood by those skilled in the art.
Claims (6)
1. A method for promoting cutting rooting and survival of a castanea mollissima tannin extract adult tree cutting shoot is characterized by comprising the following specific steps:
step one, selecting a superior tree; selecting healthy and strong Castanea mollissima superior tannin tree plants with straight trunks, small branch angles, round and uniform crown widths, and 15-40a tree ages and without quarantine diseases and insect pests;
step two, cutting collection and postharvest rejuvenation treatment; collecting newly-extracted branches facing southeast at the periphery of the crown of the castanea mollissima quel excellent tree in the last ten days of 3 months, taking the newly-extracted branches as cutting slips, firstly disinfecting the newly-extracted branches by using a carbendazim solution with the mass concentration of 0.5% for more than 24 hours, then cleaning the newly-extracted branches by using clean water, and carrying out rejuvenation treatment in an indoor shading dark environment;
step three, preparing and disinfecting a cutting medium; uniformly mixing laterite, turfy soil and calcium superphosphate according to the volume ratio of 60:30:2 to obtain a cutting matrix, spraying and disinfecting with a carbendazim solution with the mass concentration of 0.8%, piling into a conical pile, and covering and disinfecting with a plastic film for more than 24 hours to obtain a completely sterilized cutting matrix;
step four, pre-treating and trimming the cutting shoots; cutting the immature cutting slips in the girdling cut step two, cleaning the cutting slips by using clean water in a dark environment, soaking the cutting slips for more than 1h, and then disinfecting the cutting slips by using a carbendazim solution with the mass concentration of 0.5% for more than 24 h; after the pruning tool is disinfected, pruning the cutting slips in a normally-illuminated indoor environment, controlling the length of the pruned cutting slips to be 6-10cm and the thickness to be 0.4-1.6cm, reserving more than 2 bud positions, performing flat cutting on the upper gaps and performing oblique cutting on the lower gaps, and reserving 1-2 blades on the upper parts of the cutting slips to obtain the cutting slips;
step five, hormone treatment; soaking the cutting slips obtained in the fourth step in an ABT rooting agent with the concentration of 300ppm for more than 10min, taking out the cutting slips, cleaning the cutting slips with clear water, and placing the cutting slips in KNO with the mass concentration of 0.03 percent3Soaking in water solution for more than 3 h; cutting the seedlings in a cutting medium of a cutting pool at the bottom of 3 months, watering thoroughly, building an arched transparent film with the concentration of 30-40%, and watering water every 5-7 days to keep the cutting seedbed moist;
step six, temperature treatment and moisture control; spraying a bactericide every 10 days after cuttage so as to prevent the base of the cutting shoot from rotting, controlling the temperature of a cuttage seedbed to be 18-25 ℃ and the humidity to be 40-65%, and spraying urea and a potassium dihydrogen phosphate solution with the mass concentration of 0.03% every 10 days after the callus is piled up like a ball;
step seven, mycorrhiza treatment; in the period of starting roots of the Castanea mollissima extract cutting seedlings, spraying the bactericide is stopped, rhizosphere soil of the Castanea mollissima extract superior tree plants in the step I or clean soil on the surface layers of the Chinese chestnut forest and masson pine forest land is collected and covered on the bases of the Castanea mollissima extract cutting seedlings, the thickness of the roots is controlled to be 2-3cm, the environmental temperature of a cutting substrate is 20-27 ℃, and the humidity is 50-70% until the root systems of the Castanea mollissima extract develop in a net shape;
step eight, seedling management; transplanting the castanea mollissima cutting seedlings at the beginning of 7 months, controlling the distance between the seedlings to be 25-35cm, starting to perform additional fertilization 30 days after the transplanting, wherein the additional fertilization is performed for 2 times, urea solution with the mass concentration of 0.5% is applied for the first time in 8 months, and urea solution with the mass concentration of 0.5% and potassium nitrate solution with the mass concentration of 0.3% are applied for the second time in 9 months, so that the cutting seedling raising of the castanea mollissima adult trees is completed.
2. The method for promoting cutting rooting and survival of the cutting slips of the Castanea mollissima extract adult trees as claimed in claim 1, wherein in step one, Castanea mollissima extract superior plants are selected from natural inferior Castanea mollissima extract broad-leaved mixed forest with excellent ecological environment.
3. The method for promoting cutting rooting and survival of the cutting slips of the castanea mollissima extract adult trees as claimed in claim 1, wherein in the second step, the rejuvenation treatment comprises the following specific processes: wrapping the cutting slips with black plastic bags, and reserving a space with the empty margin length of 5cm in a wrapping object; girdling is carried out at the joint of the next-year old branch and the new branch of this year, the girdling width is 0.2-0.5cm, the girdling depth reaches the phloem of the cutting, and the girdling opening is exposed outside the package; thirdly, placing the cutting slips into a sucrose aqueous solution with the mass concentration of 1% for water culture, and enabling the annular peeling openings to expose the water surface to be larger than 1 cm; during water culture, ensuring that the girdling opening is kept clean, if mildew is found, immediately removing the girdling opening, replacing a sucrose aqueous solution with the mass concentration of 1% at intervals of 3-5 days, performing water culture for 15-20 days, and obtaining the young cutting when the color of all the castanopsis mollissima leaf blades in the package is changed into yellow.
4. The method for promoting cutting rooting and survival of the cutting slips of the castanea mollissima extract adult trees as claimed in claim 1, wherein in the sixth step, the bactericide is 0.5% by mass of carbendazim or chlorothalonil solution.
5. The method for promoting cutting rooting and survival of the castanea mollissima extract adult trees is characterized in that in the step eight, the castanea mollissima extract cutting seedlings are transplanted, and the distance between the seedlings is controlled to be 30 cm.
6. The method for promoting cutting rooting and survival of the cutting slips of the castanea mollissima extract adult trees as claimed in claim 1, wherein in step eight, thiophanate methyl is sprayed during the second topdressing so as to prevent and control the stem rot.
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| CN113647318A (en) * | 2021-09-06 | 2021-11-16 | 北京农学院 | Method for promoting generation of new water-cultured Chinese chestnut roots |
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