CN113647318A - Method for promoting generation of new water-cultured Chinese chestnut roots - Google Patents
Method for promoting generation of new water-cultured Chinese chestnut roots Download PDFInfo
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- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
- A01G31/06—Hydroponic culture on racks or in stacked containers
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Abstract
The invention discloses a method for promoting the generation of a new Chinese chestnut hydroponic root, which comprises the following steps: transplanting the Chinese chestnut seeds which grow the radicles after the germination acceleration treatment into a culture medium for culture, and transplanting when the Chinese chestnut seedlings grow to 4-6 true leaves; before transplanting, cleaning roots of Chinese chestnut seedlings taken out of a culture medium by clear water and Chinese chestnut water culture nutrient solution, wrapping root-stem junctions of the Chinese chestnut seedlings by using sponge after cleaning, and transplanting the Chinese chestnut seedlings into a Chinese chestnut water culture device, wherein the Chinese chestnut water culture device is internally provided with the prepared Chinese chestnut water culture nutrient solution in advance and is arranged in a culture chamber with full-sunlight conditions; the initial pH value of the Chinese chestnut water culture nutrient solution used in the Chinese chestnut water culture device is 6.5, and the Chinese chestnut water culture nutrient solution is replaced every 7 days; and performing daily management according to a daily management mode corresponding to the type of the culture room until the chestnut plants with the new roots meeting the requirements are obtained. The Chinese chestnut can be cultured in a water culture mode, and Chinese chestnut plants with root systems meeting experimental requirements are obtained.
Description
Technical Field
The invention relates to the field of Chinese chestnut cultivation, in particular to a method for promoting the generation of new water-cultured Chinese chestnut roots.
Background
At present, most of basic scientific research experimental materials of Chinese chestnuts adopt tissue culture nursery stocks of solid culture media or nursery stocks cultured by grass beach vermiculite in fields, and have a plurality of limitations in the aspect of scientific research treatment. On the one hand, many researches in the aspect of the Chinese chestnut root system need to take out the root system from the growing solid medium, but the solid medium of the root system growth is relatively hard, easily damages and stresses the root system, destroys the integrity of the root system, and can influence the physiological characteristics and biochemical indexes of the root system to a certain extent. On the other hand, in the salt and alkali stress treatment test of the Chinese chestnut, the Chinese chestnut root systems at different growth stages need to be set with different salt stress concentrations, pH values and different plant hormones for treatment, and the development of different test treatments needs to completely and completely return the Chinese chestnut root systems growing to a certain stage to a solid growth medium, so that the practical operation is basically infeasible. In addition, the components of the solid medium for the root growth of the Chinese chestnut and the environmental regulation are relatively difficult. These all greatly limit the progress of basic scientific research on chestnuts.
At present, no relevant report about the success of Chinese chestnut water culture exists, and mainly due to the growth habit, the physiological characteristics, the special environmental adaptability and the like of Chinese chestnuts, other plant water culture technical systems cannot be applied to Chinese chestnuts. Therefore, how to provide a water culture technology suitable for Chinese chestnuts, in particular to a method for promoting the generation of new water culture roots of Chinese chestnuts is a problem to be solved.
In view of the above, the present invention is particularly proposed.
Disclosure of Invention
The invention aims to provide a method for promoting the generation of a Chinese chestnut water culture new root, which can promote the growth of the Chinese chestnut water culture new root to obtain a Chinese chestnut water culture plant and further solve the technical problems in the prior art.
The purpose of the invention is realized by the following technical scheme:
the embodiment of the invention provides a method for promoting the generation of a new water-cultured Chinese chestnut root, which comprises the following steps:
transplanting the Chinese chestnut seeds which grow the radicles after the germination acceleration treatment into a culture medium for culture, and transplanting when the Chinese chestnut seedlings grow to 4-6 true leaves;
before transplanting, firstly cleaning the roots of Chinese chestnut seedlings taken out of a culture medium by using tap water to completely expand the root systems, then continuously cleaning the roots of the cleaned Chinese chestnut seedlings by using a Chinese chestnut water culture nutrient solution with a pre-prepared concentration of 1/2, wrapping root-tuber junctions of the Chinese chestnut seedlings by using sponge after cleaning, and then transplanting the Chinese chestnut seedlings into a Chinese chestnut water culture device, wherein the Chinese chestnut water culture device is internally provided with the Chinese chestnut water culture nutrient solution in advance and is arranged in a culture room with full-sunlight conditions;
the initial pH value of the Chinese chestnut water culture nutrient solution used in the Chinese chestnut water culture device is 6.5, and the Chinese chestnut water culture nutrient solution is changed every 7 days; and performing daily management on the water-cultured Chinese chestnut according to a daily management mode corresponding to the type of the culture room until a Chinese chestnut plant with a new rooting line meeting the requirement is obtained.
Compared with the prior art, the method for promoting the generation of the new hydroponic roots of the Chinese chestnuts, provided by the invention, has the beneficial effects that:
by accelerating germination, transplanting when the chestnut seedlings grow to 4-6 true leaves, and matching with cleaning before transplanting, the water culture can be carried out in a chestnut water culture device through chestnut water culture nutrient solution, the method can promote the growth of new roots of the chestnut in water culture, and 40 new roots per plant can grow in 14 days of water culture of the chestnut, so that the chestnut plant with the new roots meeting the planting requirements can be obtained.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a flow chart of a method for promoting the generation of new Chinese chestnut hydroponic roots according to an embodiment of the invention;
FIG. 2 is a schematic structural diagram of a Chinese chestnut water culture device in the method provided by the embodiment of the invention;
in the figure: 1-a water culture tank; 2-perforated foam board; 3-light-proof silver foil paper; 4-adjusting the support; 5, an air pump; 6-control device.
Detailed Description
The technical scheme in the embodiment of the invention is clearly and completely described below by combining the attached drawings in the embodiment of the invention; it is to be understood that the described embodiments are merely exemplary of the invention, and are not intended to limit the invention to the particular forms disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without any creative effort, shall fall within the protection scope of the present invention.
The terms that may be used herein are first described as follows:
the term "and/or" means that either or both can be achieved, for example, X and/or Y means that both cases include "X" or "Y" as well as three cases including "X and Y".
The terms "comprising," "including," "containing," "having," or other similar terms of meaning should be construed as non-exclusive inclusions. For example: including a feature (e.g., material, component, ingredient, carrier, formulation, material, dimension, part, component, mechanism, device, process, procedure, method, reaction condition, processing condition, parameter, algorithm, signal, data, product, or article of manufacture), is to be construed as including not only the particular feature explicitly listed but also other features not explicitly listed as such which are known in the art.
The term "consisting of … …" is meant to exclude any technical feature elements not explicitly listed. If used in a claim, the term shall render the claim closed except for the technical features explicitly listed except for conventional impurities associated therewith. If the term occurs in only one clause of the claims, it is defined only to the elements explicitly recited in that clause, and elements recited in other clauses are not excluded from the overall claims.
The term "parts by mass" is intended to indicate a mass ratio relationship between a plurality of components, for example: if X component is X parts by mass and Y component is Y parts by mass, the mass ratio of the X component to the Y component is X: Y; 1 part by mass may represent any mass, for example: 1 part by mass may be expressed as 1kg or 3.1415926 kg. The sum of the parts by mass of all the components is not necessarily 100 parts, and may be more than 100 parts, less than 100 parts, or equal to 100 parts. Parts, ratios and percentages described herein are by mass unless otherwise indicated.
Unless expressly stated or limited otherwise, the terms "mounted," "connected," and "secured," etc., are to be construed broadly, as for example: can be fixedly connected, can also be detachably connected or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms herein can be understood by those of ordinary skill in the art as appropriate.
When concentrations, temperatures, pressures, dimensions, or other parameters are expressed as ranges of values, the ranges are to be understood as specifically disclosing all ranges formed from any pair of upper and lower values, preferred values within the range, regardless of whether ranges are explicitly recited; for example, if a numerical range of "2 ~ 8" is recited, then the numerical range should be interpreted to include ranges of "2 ~ 7", "2 ~ 6", "5 ~ 7", "3 ~ 4 and 6 ~ 7", "3 ~ 5 and 7", "2 and 5 ~ 7", and the like. Unless otherwise indicated, the numerical ranges recited herein include both the endpoints thereof and all integers and fractions within the numerical range.
The terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," and the like are used in an orientation or positional relationship that is indicated based on the orientation or positional relationship shown in the drawings for ease of description and simplicity of description only, and are not intended to imply or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be considered limiting herein.
The method for promoting the generation of the new hydroponic roots of the Chinese chestnut provided by the invention is described in detail below. Details which are not described in detail in the embodiments of the invention belong to the prior art which is known to the person skilled in the art. The examples of the present invention, in which specific conditions are not indicated, were carried out according to the conventional conditions in the art or conditions suggested by the manufacturer. The reagents or instruments used in the examples of the present invention are not specified by manufacturers, and are all conventional products available by commercial purchase.
As shown in fig. 1, the method for promoting the generation of the new hydroponic roots of the Chinese chestnut comprises the following steps:
transplanting the Chinese chestnut seeds which grow the radicles after the germination acceleration treatment into a culture medium for culture, and transplanting when the Chinese chestnut seedlings grow to 4-6 true leaves;
before transplanting, putting the roots of the Chinese chestnut seedlings taken out of a culture medium into a basin filled with tap water for nondestructive cleaning, expanding the root systems completely, putting the roots of the cleaned Chinese chestnut seedlings into a Chinese chestnut water culture nutrient solution with 1/2 concentration for continuous cleaning, wrapping root-tuber junctions of the Chinese chestnut seedlings with sponge after cleaning, and transplanting the Chinese chestnut seedlings into a Chinese chestnut water culture device, wherein the Chinese chestnut water culture nutrient solution prepared in advance is filled in the Chinese chestnut water culture device, and the Chinese chestnut water culture device is arranged in a culture chamber with full-sunlight conditions;
the initial pH value of the Chinese chestnut water culture nutrient solution used in the Chinese chestnut water culture device is 6.5, and the Chinese chestnut water culture nutrient solution is changed every 7 days; and performing daily management on the water-cultured Chinese chestnut according to a daily management mode corresponding to the type of the culture room until a Chinese chestnut plant with a new rooting line meeting the requirement is obtained. The meeting requirement can be meeting experimental requirement or planting requirement.
Preferably, before transplanting, the roots of the Chinese chestnut seedlings taken out of the culture medium are subjected to nondestructive cleaning by using tap water, the root systems are completely unfolded, and then the roots of the cleaned Chinese chestnut seedlings are subjected to nondestructive cleaning continuously by using a Chinese chestnut water culture nutrient solution with the pre-prepared concentration of 1/2.
In the method, the Chinese chestnut water culture nutrient solution consists of the following elements in volume ratio:
macroelements: KH (Perkin Elmer)2PO4 0.3mM、KNO3 1.6mM、MgSO40.7mM and Ca (NO)3)2 1.7mM；
Trace elements: h3BO3 15.41μM、MnCl2·4H2O 3.05μM、ZnSO4·7H2O 0.25μM、CuSO4·5H2O 0.11μM、NaMoO4·H2O0.03. mu.M and FeSO4 & 7H2O-Na2EDTA 0.15mM；
Adjusting the initial pH value of the Chinese chestnut water culture nutrient solution to 6.5 by using a KOH solution.
Preferably, the depth of the chestnut water culture nutrient solution in the chestnut water culture device is 10-15 cm.
As shown in fig. 2, in the above method, the chestnut hydroponics device includes:
the Chinese chestnut water culture nutrient solution is arranged in the water culture box;
the adjusting bracket is arranged in the water culture box, and the top supporting end of the adjusting bracket is higher than the liquid level of the chestnut water culture nutrient solution;
the perforated foam plate is supported by the adjusting bracket and is arranged in the middle part in the hydroponic tank;
the peripheral wall of the water culture box is provided with the light-shading silver foil paper which can shade the Chinese chestnut water culture nutrient solution in the water culture box;
an air outlet pipe of the air pump is connected to the Chinese chestnut water culture nutrient solution in the water culture box, and the air pump is electrically connected with the control device.
In the Chinese chestnut water culture device, the control device can control the air pump to convey air once every 20 minutes, and the water pump is started all day after 20 minutes of conveying.
In the method, the top supporting end of the adjusting bracket is 3-4cm higher than the Chinese chestnut water planting nutrient solution level, so that the height of the perforated foam plate in the water planting box can be adjusted conveniently according to the root length of the Chinese chestnut seedlings, the Chinese chestnut seedlings can be supported conveniently, and shading is facilitated. Preferably, the adjusting bracket can adopt an iron wire bracket, a stainless steel wire bracket and the like, so that the supporting height can be conveniently adjusted.
In the method, a plurality of cultivation through holes are uniformly formed in the perforated foam plate, the interval between every two adjacent cultivation through holes is 15cm, and the aperture of each cultivation through hole is 3.5 cm.
In the method, the culture medium is prepared from river sand and vermiculite according to the ratio of 1: 1 volume ratio.
In the above method, the types of the culture chamber include:
any one of a glass greenhouse, an incubator and an artificial climate chamber;
if the type of the culture room is a glass greenhouse, the corresponding daily management mode is as follows: natural illumination is adopted, the temperature is maintained at 20-35 ℃, and the humidity is maintained at 55-85%;
if the culture room is an incubator or a phytotron, the illumination is 120-160 μ M/M2And/s, maintaining the temperature at 20-35 ℃ and the humidity at 55-85%.
In the method, the pregermination treatment is carried out by sand storage or cold storage treatment at 2-6 ℃.
In conclusion, the method provided by the embodiment of the invention has the advantages that through two times of non-destructive cleaning before germination accelerating, culture treatment and transplanting, the Chinese chestnut water culture nutrient solution and the Chinese chestnut water culture device with specific compositions and corresponding daily management, the Chinese chestnut seedlings can well generate new roots, various requirements on subsequent Chinese chestnut experiments are met, and the problem that Chinese chestnuts are difficult to generate new roots and are difficult to obtain plants meeting experimental requirements through common water culture is solved.
In order to more clearly show the technical scheme and the technical effects provided by the invention, the method for promoting the generation of the new hydroponic roots of the Chinese chestnut provided by the embodiment of the invention is described in detail by specific embodiments.
Example 1
As shown in fig. 1, the embodiment of the invention provides a Chinese chestnut water planting method, which can promote the generation of new roots of water-planted Chinese chestnuts through various measures and provide technical support for deeply researching mechanisms of Chinese chestnut response to saline-alkali stress and the like, and the method comprises the following steps:
a first part: preparing a Chinese chestnut water culture nutrient solution:
macroelements: KH (Perkin Elmer)2PO4 0.3mM,KNO3 1.6mM,MgSO4 0.7mM,Ca(NO3)2 1.7mM；
Trace elements: h3BO3 15.41μM,MnCl2·4H2O 3.05μM,ZnSO4·7H2O 0.25μM,CuSO4·5H2O 0.11μM,NaMoO4·H2O 0.03μM,FeSO4·7H2O-Na2EDTA 0.15mM。
Adjusting the pH value of the Chinese chestnut water culture nutrient solution to 6.5 by using a KOH solution;
a second part: structure of Chinese chestnut hydroponics device (see fig. 2):
assembly of: air pump, water planting case, controlling means, cystosepiment of 4cm thickness, sponge of 4cm thickness, light-resistant silver-foil paper, regulation support etc..
And a third part: place and installation are placed to chinese chestnut hydroponics device:
selecting a field: generally, a glass greenhouse, a culture room or an artificial climate room and the like are selected to meet the full-sunlight condition.
Installation of the hydroponic device: the specification of the water culture box is 100cm in length, 40cm in width and 30cm in height, and the foam board is cut according to the specification of the water culture box, so that the size of the foam board is consistent as much as possible. In order to ensure the light-proof of the root system growth environment, the gap between the foam plate and the water culture box is shielded and sealed by silver foil paper;
the depth of the solution in the water culture box reaches 10-15cm, the foam board is punched by punching equipment according to the size of the Chinese chestnut seeds, the diameter of each hole is generally 3.5cm, and the distance between every two holes is 15 cm; air is delivered into the hydroponic solution through an air pump, so that the oxygen content of the hydroponic solution is sufficient, the air is delivered every 20 minutes, and the air pump is started all day long; the periphery, the top and the bottom of the water culture box are all made of shading silver-foil paper, so that the water culture solution is protected from light; an iron wire frame is placed in the water culture box and is 3-4cm higher than the water surface; the base part of the Chinese chestnut seedling rootstock is wrapped by the sponge and passes through the small holes of the foam board, and the nuts are placed at the lower part of the foam board; the root system 1/3 is partially exposed in the air;
the fourth part: transplanting the water culture seedlings:
culturing and transplanting water culture seedlings: and (3) germinating by sand storage or treatment in a cold storage at 2-6 ℃, wherein the radicle grows transplanted river sand and vermiculite 1: 1, culturing in a mixed culture medium, and transplanting when the chestnut seedlings grow to 4-6 true leaves in about 2 weeks. During transplanting, a pot of usually irrigated tap water and a pot of 1/2-concentration Chinese chestnut water culture nutrient solution are prepared, the root of a Chinese chestnut seedling taken out of a culture medium is placed in the pot containing tap water for cleaning, the root system is completely unfolded, the action needs to be gentle and gentle to avoid damaging the root, and then the Chinese chestnut seedling is placed in 1/2-concentration Chinese chestnut water culture nutrient solution for continuous cleaning, and the action needs to be gentle and gentle. Wrapping the root-stem junction of the chestnut seedlings with the prepared sponge in the solution, and then putting the chestnut seedlings into a foam plate, wherein the specific requirements are described in the mounting part of the hydroponic device.
The fifth part is that: daily management of water culture seedlings:
chinese chestnut water culture nutrient solution: the pH value of the initial Chinese chestnut water culture nutrient solution is adjusted to 6.5, and the Chinese chestnut water culture nutrient solution is replaced once every 7 days.
Daily management: and (5) normally managing the water culture seedlings. In a glass greenhouse, natural illumination can be carried out without any shading and light supplementing treatment, the temperature is maintained at 20-35 ℃, the temperature is required to be heated when the temperature is lower than 20 ℃, the temperature is required to be cooled when the temperature is higher than 35 ℃, the temperature is not required to be increased or decreased in the normal growing season of the Chinese chestnut, and the humidity is maintained at 55%85% of the total weight of the composition. In an incubator or a climatic chamber, the illumination is 120-160 mu M/M2The temperature is maintained at 20-35 ℃ and the humidity is maintained at 55-85%;
the Chinese chestnut seedlings are optimally transplanted when 4 true leaves are used.
The method can obtain Chinese chestnut plants by water culture, and can be applied to research of the physiology and molecular mechanism of Chinese chestnuts suitable for salt and alkali stress. Meanwhile, the water culture method is suitable for researching the adverse physiological mechanisms of the Chinese chestnut, such as a nutrient stress mechanism, a hormone signal response mechanism, an alkali stress mechanism and the like. The water culture method can strictly control the growth environment, growth medium components and the like of the Chinese chestnut, and is an ideal technology for developing the basic research science of the Chinese chestnut.
Examples
The embodiment provides a method for promoting the generation of new roots of Chinese chestnut water culture, which can determine the influence of high pH value treatment on the growth of Chinese chestnuts, and comprises the following steps:
(1) and (3) test treatment:
the water culture method of the invention is adopted, Chinese chestnut variety Jingshuhong is used as a test material, and the Chinese chestnut variety Jingshuhong is sowed in river sand, vermiculite and turfy soil 1: 1: 1, taking Chinese chestnut seedlings which have consistent growth vigor and 4 true leaves after 2 weeks on a mixed culture medium, and transplanting the Chinese chestnut seedlings in a water culture device according to the technology established by the invention. This experiment set up two treatments: sampling normal nutrient solution (CK, pH value of 6.5) and high pH value nutrient solution (pH value of 8.5) respectively in 7 days, 14 days, 21 days and 28 days, analyzing chlorophyll content in leaves, and determining activity of root system, active oxygen and antioxidant enzyme.
(2) Test data:
tables 1-5 show the influence of pH treatment on the growth amount of Chinese chestnut roots and leaves, the activity of the roots, the chlorophyll content of leaves, carotenoid, malondialdehyde and SOD, and the data are subjected to significance analysis.
Table 1 shows the influence of pH on the amount of chestnut growth
Table 2 shows the influence of pH value on chlorophyll content of chestnut leaves
Table 3 shows the influence of pH on the activity of chestnut root system
Table 4 shows the influence of pH on the malondialdehyde content in the roots and leaves of Castanea mollissima
Table 5 shows the effect of pH on SOD activity of chestnut roots and leaves
Table 1 shows that the high pH value severely inhibits the growth and development of Chinese chestnuts, the growth of overground part tissues and underground part tissues is inhibited, and particularly the growth amount of underground part root systems is severely inhibited. Table 2 shows that under the stress of high pH value, the content of chlorophyll a, chlorophyll b and carotenoid of the chestnut is reduced. Table 3 shows that root vigor is significantly reduced under high pH stress and is more severe with stress time. As can be seen from tables 4 and 5, the malondialdehyde content of the chestnut roots and leaves was increased under stress conditions, while the SOD activity was increased. Therefore, the growth of the Chinese chestnut is remarkably inhibited under the stress of high pH value. The Chinese chestnut water culture technical system established by the invention can be used for researching the Chinese chestnut alkali stress mechanism. The technical system established by the invention promotes the generation of new roots of the Chinese chestnut.
In conclusion, the Chinese chestnut water culture method disclosed by the embodiment of the invention overcomes the characteristic that the root system is easy to damage in the solid medium culture technology, better protects the root system, and more importantly, is convenient for regulating and controlling the environmental conditions and processing factors of the Chinese chestnut and is convenient for experimental processing. Meanwhile, the invention greatly promotes the generation of the Chinese chestnut water culture new root by regulating and controlling the components and the concentration of the nutrient solution and establishing a set of unique water culture treatment steps, thereby facilitating the development of the basic scientific research of Chinese chestnuts.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims. The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.
Claims (9)
1. A method for promoting the generation of a new water-cultured Chinese chestnut root is characterized by comprising the following steps:
transplanting the Chinese chestnut seeds which grow the radicles after the germination acceleration treatment into a culture medium for culture, and transplanting when the Chinese chestnut seedlings grow to 4-6 true leaves;
before transplanting, firstly cleaning the roots of Chinese chestnut seedlings taken out of a culture medium by using tap water to completely expand the root systems, then continuously cleaning the roots of the cleaned Chinese chestnut seedlings by using a Chinese chestnut water culture nutrient solution with a pre-prepared concentration of 1/2, wrapping root-tuber junctions of the Chinese chestnut seedlings by using sponge after cleaning, and then transplanting the Chinese chestnut seedlings into a Chinese chestnut water culture device, wherein the Chinese chestnut water culture device is internally provided with the pre-prepared Chinese chestnut water culture nutrient solution and is arranged in a culture chamber with full-sunlight conditions;
the initial pH value of the Chinese chestnut water culture nutrient solution used in the Chinese chestnut water culture device is 6.5, and the Chinese chestnut water culture nutrient solution is replaced every 7 days; and performing daily management on the water-cultured Chinese chestnut according to a daily management mode corresponding to the type of the culture room until a Chinese chestnut plant with a new root system meeting the requirement is obtained.
2. The method for promoting the generation of the water culture new roots of the Chinese chestnuts according to claim 1, wherein the water culture nutrient solution of the Chinese chestnuts consists of the following elements in volume ratio:
macroelements: KH (Perkin Elmer)2PO4 0.3mM、KNO3 1.6mM、MgSO40.7mM and Ca (NO)3)2 1.7mM；
Trace elements: h3BO3 15.41μM、MnCl2·4H2O 3.05μM、ZnSO4·7H2O 0.25μM、CuSO4·5H2O0.11μM、NaMoO4·H2O0.03. mu.M and FeSO4 & 7H2O-Na2EDTA 0.15mM；
Adjusting the initial pH value of the Chinese chestnut water culture nutrient solution to 6.5 by using a KOH solution.
3. The method for promoting the generation of the new hydroponic roots of the Chinese chestnuts according to claim 1 or 2, wherein the depth of the nutrient solution for the Chinese chestnut hydroponics in the Chinese chestnut hydroponics device is 10-15 cm.
4. The method for promoting the generation of new roots in the water culture of Chinese chestnuts according to claim 1 or 2, wherein the chestnut water culture device comprises:
the device comprises a water culture box, a perforated foam plate, photophobic silver foil paper, an air pump, a control device and an adjusting bracket; wherein the content of the first and second substances,
the Chinese chestnut water culture nutrient solution is arranged in the water culture box;
the adjusting bracket is arranged in the water culture box, and the top supporting end of the adjusting bracket is higher than the liquid level of the chestnut water culture nutrient solution;
the perforated foam plate is supported by the adjusting bracket and is arranged in the middle part in the hydroponic tank;
the peripheral wall of the water culture box is provided with the light-shading silver foil paper which can shade the Chinese chestnut water culture nutrient solution in the water culture box;
an air outlet pipe of the air pump is connected to the Chinese chestnut water culture nutrient solution in the water culture box, and the air pump is electrically connected with the control device.
5. The method for promoting the generation of hydroponic new roots of Chinese chestnuts according to claim 4, wherein the control device can control the air pump to deliver air every 20 minutes, and every 20 minutes.
6. The method for promoting the generation of the new hydroponic roots of the Chinese chestnuts according to claim 4, wherein the supporting end at the top of the adjusting bracket is 3-4cm higher than the liquid level of the Chinese chestnut hydroponic nutrient solution;
a plurality of cultivation through holes are uniformly formed in the perforated foam plate, the interval between every two adjacent cultivation through holes is 15cm, and the aperture of each cultivation through hole is 3.5 cm.
7. The method for promoting the generation of the hydroponic new roots of the Chinese chestnut according to the claim 1 or 2, characterized in that the culture medium is prepared by mixing river sand and vermiculite according to the ratio of 1: 1 by volume ratio.
8. The method for promoting the hydroponics of new roots of chestnuts according to claim 1 or 2, wherein the types of the culture chamber include:
any one of a glass greenhouse, an incubator and an artificial climate chamber;
if the type of the culture room is a glass greenhouse, the corresponding daily management mode is as follows: natural illumination is adopted, the temperature is maintained at 20-35 ℃, and the humidity is maintained at 55-85%;
if the culture room is an incubator or a phytotron, the illumination is 120-160 μ M/M2And/s, maintaining the temperature at 20-35 ℃ and the humidity at 55-85%.
9. The method for promoting the generation of the water-cultured new roots of the Chinese chestnuts according to claim 1 or 2, wherein the germination accelerating treatment is carried out by sand storage or cold storage treatment at 2-6 ℃.
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