CN111418366A - Tilia miqueliana layering propagation method - Google Patents
Tilia miqueliana layering propagation method Download PDFInfo
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- CN111418366A CN111418366A CN202010270772.2A CN202010270772A CN111418366A CN 111418366 A CN111418366 A CN 111418366A CN 202010270772 A CN202010270772 A CN 202010270772A CN 111418366 A CN111418366 A CN 111418366A
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- layering
- tilia miqueliana
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- seedlings
- propagation
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- 241000328973 Tilia miqueliana Species 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 22
- 241000196324 Embryophyta Species 0.000 claims abstract description 14
- 238000005520 cutting process Methods 0.000 claims description 9
- 239000011159 matrix material Substances 0.000 claims description 9
- 238000005507 spraying Methods 0.000 claims description 9
- 239000008139 complexing agent Substances 0.000 claims description 8
- 239000002689 soil Substances 0.000 claims description 8
- 230000004720 fertilization Effects 0.000 claims description 6
- 239000000758 substrate Substances 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 239000003415 peat Substances 0.000 claims description 4
- 239000010451 perlite Substances 0.000 claims description 4
- 235000019362 perlite Nutrition 0.000 claims description 4
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 3
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 3
- 239000006013 carbendazim Substances 0.000 claims description 3
- 238000007726 management method Methods 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000009966 trimming Methods 0.000 claims description 3
- 239000004563 wettable powder Substances 0.000 claims description 3
- 241000132152 Polymyxa Species 0.000 claims description 2
- 239000003895 organic fertilizer Substances 0.000 claims description 2
- 238000013138 pruning Methods 0.000 claims description 2
- 238000003672 processing method Methods 0.000 claims 1
- 238000010187 selection method Methods 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 5
- 241000894007 species Species 0.000 description 12
- 238000000855 fermentation Methods 0.000 description 5
- 230000004151 fermentation Effects 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical group C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 241000194105 Paenibacillus polymyxa Species 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 241000102006 Aporosa miqueliana Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- -1 layering Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 230000021217 seedling development Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/20—Layering
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Soil Sciences (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of plant seedling propagation, and discloses a Tilia miqueliana Maxim layering propagation method, which comprises the following steps: the method is simple to operate, large-size Tilia miqueliana seedlings can be cultivated, the female parent sprout tiller branches can be largely utilized, and the cost is reduced for seedling cultivation. The rooting time of layering is 55d, the survival rate is more than 90%, the vacancy of market resources of Tilia miqueliana Maxim is effectively solved, and the application prospect is wide.
Description
Technical Field
The invention relates to a Tilia miqueliana layering rapid propagation method, belonging to the technical field of woody plant seedling propagation.
Background
Tilia miqueliana Maxim (A. miqueliana Maxim.)Tilia miqueliana) Is deciduous tree with a height of 20 m, and is a good tree species integrating tree species of timber, honey source plants and garden appreciation. Tilia miqueliana is positioned as a landscape tree species and an afforestation tree species which are mainly developed in forestry development planning of our province and forest seedling development planning of Jiangsu province (2008) 2015), is also one of 10 preferable recommended precious tree species in a 'Jiangsu province precious material cultivation action scheme' proposed by Jiangsu province government in 2016 years, wherein the landscape tree species and the afforestation tree species are mainly developed in green land system planning of Nanjing City (2013-2020) and the pavement tree species planning of Nanjing City (2014) and are to be mainly popularized and applied. However, the tree species is difficult to breed, so that the popularization and application are always greatly limited, and the tree species cannot be effectively popularized.
The branches are also called as "layering". A plant propagation technique features that the plant branch is annularly peeled or carved to deep reach the bark layer, treated by rooting hormone and buried in soil, and the root end of plant is exposed to ground. The branch pressing seedling raising is an effective way for farmers to rapidly propagate Tilia miqueliana Maxim. Although some researches on the rapid propagation technology of tilia miqueliana are currently carried out, most researches are focused on cutting propagation, cutting slips and matrixes are separated in the cutting process, the survival rate can be improved only by controlling the humidity and the temperature of environmental conditions, the difficulty is high, the seedling production cannot be effectively guaranteed, and the development of the tilia miqueliana seedling industry is limited to a great extent. Therefore, the number of seedlings of the tree species is very small at home, and related researches show that the seed germination of Tilia miqueliana Maxim is difficult, so that the resource is very lacking, and the application is 'available and unavailable'.
According to the growth characteristics of Tilia miqueliana seeds, the sprouts at the seedling stage are developed, and in order to ensure that the tree form needs to trim the bottom branches for a long time and cause branch waste, if the sprouts and the bottom side branches are utilized by a layering propagation technology, the resources are fully utilized, and the seedling production speed can be improved.
In conclusion, the propagation of tilia miqueliana by utilizing layering is one of the important ways for solving the resource shortage of the species, and has very important significance for the development, popularization and utilization of tilia miqueliana.
Disclosure of Invention
The invention mainly solves the technical problem of disclosing a Tilia miqueliana layering propagation method.
The technical scheme is as follows:
A) female parent selection and culture:
the female parent selects 3-5 year seedlings, sufficient organic fertilizer is applied in 11 months in the last year, and meanwhile, pruning is carried out to remove apical dominance and strengthen sprout cultivation.
B) Layering selection and processing:
selecting current-year sprouting branches or lower lateral branches as layering, performing disinfection treatment 28 days before layering treatment, spraying 50% carbendazim wettable powder 500 times liquid, spraying for 4 times, spraying for 100 ml/plant each time, and performing time interval 7 days.
C) Layering matrix selection:
selecting perlite with a volume ratio of 1:1: 1: peat soil: yellow core soil is used as a substrate.
D) The layering method comprises the following steps:
pressing at the end of 6 months after the branches are semi-lignified and the axillary buds are full, selecting a thick branch, trimming redundant leaves, keeping 1 complete leaf, performing annular peeling on the bark of the branch every 2-3 axillary buds, immersing the branch in a rooting complexing agent, treating for 1min, embedding the branch in a matrix, and completely pouring 500 ml of the rooting complexing agent to fix, wherein the rooting complexing agent is IBA solution with the concentration of 2000ppm and 1 × 108Paenibacillus polymyxa at cfu/L concentration.
E) Layering seedling transplanting and fertilizing management:
and D) after the medium-pressure strips are rooted, carrying out foliar fertilization on the layering seedlings, 1 time every 2 weeks, cutting the layering seedlings and the female parents into single plants after the leaves fall in autumn, and carrying out container cultivation.
Compared with the prior art, the invention has the beneficial effects that:
the tilia miqueliana is bred by a layering method, large-size tilia miqueliana seedlings can be quickly and effectively obtained, and the problem of resource shortage of tilia miqueliana is solved; through groping of the substrate, layering, hormone conditions and a transplanting method after survival, the layering seedling of tilia miqueliana can quickly reach the specification of the seedling (the height of the seedling is more than 50 cm) after survival, and the high-quality tilia miqueliana seedling can be efficiently obtained. Meanwhile, after layering seedlings survive, the new branches can be used as cutting slips for cuttage (the rooting rate of the cutting slips of the parent body growing for more than 3 years is extremely low). Compared with tissue culture seedlings and cutting seedlings, the layering seedlings cultivated in the same year have developed root systems and vigorous growth, the cultivation time is shortened by 2-3 years, and the market competitive advantage is greatly improved.
Detailed Description
The method for tilia miqueliana layering propagation is characterized by comprising the following steps:
selecting and culturing female parents, selecting and processing layering B, selecting layering matrix C, layering D, and transplanting, fertilizing and managing layering seedlings E.
In 11 months in 2018, the female parent is a 3-year-old seedling, the female parent of Tilia miqueliana Maxim is recutted, the female parent of Tilia miqueliana Maxim is trimmed at a position 30cm away from the base of the trunk, and 2-3 axillary buds are reserved.
The method for culturing the female parent in the step A) comprises the following steps: and (3) container cultivation, wherein the culture medium is prepared by selecting perlite with the volume ratio of 1:1: 1: peat soil: the base fertilizer is organic slow release fertilizer with the weight percentage of 10 percent added in the matrix; before shoots germinate in 2019 in 3 months, placing the scion female parent in a greenhouse or a seedling raising greenhouse for germination accelerating culture; the foliar fertilization starts in 4 months, and the fertilizer is fertilized once every 2 weeks, using 1/4Hoagland nutrient solution.
Step B) layering selection and processing: selecting current-year sprouting branches or lower lateral branches as layering, performing disinfection treatment 28 days before layering treatment, spraying 50% carbendazim wettable powder 500 times liquid, spraying for 4 times, spraying for 100 ml/plant each time, and performing time interval 7 days.
Step C) layering matrix selection: selecting perlite with a volume ratio of 1:1: 1: peat soil: yellow core soil is used as a substrate, after the substrate is uniformly mixed, the substrate is paved into a seedling raising box along the growth direction of branches, the width and the height of the seedling raising box are 10cm, the length of the seedling raising box is determined according to the length of the branches, and the left side surface and the right side surface of the seedling raising box are fixed by adopting wood boards.
Selecting and processing the cutting shoots in the step D): layering can be carried out after branches are semi-lignified and axillary buds are full in 6 months in 2019; selecting a thick and strong branch, trimming redundant leaves, keeping 1 complete leaf, immersing the branch into a rooting complexing agent every 2-3 axillary buds after girdling the bark of the branch, treating for 1min, burying in a matrix, and completely pouring 500 ml of the rooting complexing agent for fixation. Layering 40 Tilia miqueliana plants, wherein each plant is treated with 3 branches, and the total number of the branches is 120.
The compound rooting preparation comprises: the IBA solution and the microbial fermentation broth (containing dipotassium hydrogen phosphate and Paenibacillus polymyxa fermentation broth in a weight ratio of 1: 1) with a concentration of 2000ppm in a volume ratio of 1: 1.
The preparation method of the compound rooting preparation comprises the steps of activating and culturing the paenibacillus polymyxa according to a conventional mode until the number of live bacteria in a bacterial liquid reaches 108One/gram of the fermentation broth is obtained. Mixing dipotassium phosphate with fermentation liquor according to the mass ratio of 1:1, and then mixing the fermentation liquor with indolebutyric acid according to the volume ratio of 1:1 to obtain the compound.
Step E), layering seedling transplanting fertilization management:
and (3) rooting sequentially after 55 days of layering culture, wherein the rooting rate reaches 84% after 100 days, foliar fertilization is carried out on the new leaves of the layering seedlings, 1 time is carried out every 2 weeks, and after leaves fall in autumn, the layering seedlings and the female parents are cut into single plants for container culture. The transplanting survival rate is 98%.
By the method, large-size (the plant height is more than 50 cm) Tilia miqueliana seedlings can be rapidly cultivated, and the market blank can be greatly filled.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, it is intended that all such modifications, improvements and extensions that do not depart from the spirit of the invention, be considered within the scope of the invention as claimed.
Claims (7)
1. The method for tilia miqueliana layering propagation is characterized by comprising the following steps:
selecting and culturing female parents, selecting and processing layering B, selecting layering matrix C, layering D, and transplanting, fertilizing and managing layering seedlings E.
2. The method for tilia miqueliana layering propagation according to claim 1, wherein the female parent in step a) is selected from 3-5 year old seedlings, and in 11 months of the previous year, organic fertilizer is applied, and pruning is performed to remove apical dominance and enhance tillering cultivation.
3. The method for tilia miqueliana layering propagation according to claim 1, wherein the layering selection and processing method in step B) is as follows: selecting current-year sprouting branches or lower lateral branches as layering, performing disinfection treatment 28 days before layering treatment, spraying 50% carbendazim wettable powder 500 times liquid, spraying for 4 times, spraying for 100 ml/plant each time, and performing time interval 7 days.
4. The method for tilia miqueliana layering propagation according to claim 1, wherein the layering matrix in step C) is selected from the group consisting of: selecting perlite with a volume ratio of 1:1: 1: peat soil: yellow core soil is used as a substrate.
5. The method for layering propagation of tilia miqueliana as claimed in claim 1, wherein the layering method in step D) is: layering at the end of 6 months after the branches are semi-lignified and the axillary buds are full; selecting a thick and strong branch, trimming redundant leaves, keeping 1 complete leaf, immersing the branch into a rooting complexing agent every 2-3 axillary buds after girdling the bark of the branch, treating for 1min, burying in a matrix, and completely pouring 500 ml of the rooting complexing agent for fixation.
6. The method for tilia miqueliana layering propagation according to claim 1, wherein the layering seedling transplanting fertilization management method in step E) comprises: and D) after the medium-pressure strips are rooted, carrying out foliar fertilization on the layering seedlings, 1 time every 2 weeks, cutting the layering seedlings and the female parents into single plants after the leaves fall in autumn, and carrying out container cultivation.
7. The method of claim 5The Tilia miqueliana layering propagation method is characterized in that the rooting complexing agent is IBA solution with the concentration of 2000ppm and 1 × 108Paenibacillus polymyxa at cfu/L concentration.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010270772.2A CN111418366A (en) | 2020-04-08 | 2020-04-08 | Tilia miqueliana layering propagation method |
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| CN202010270772.2A CN111418366A (en) | 2020-04-08 | 2020-04-08 | Tilia miqueliana layering propagation method |
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| CN111418366A true CN111418366A (en) | 2020-07-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202010270772.2A Pending CN111418366A (en) | 2020-04-08 | 2020-04-08 | Tilia miqueliana layering propagation method |
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| Country | Link |
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| CN (1) | CN111418366A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111919608A (en) * | 2020-08-05 | 2020-11-13 | 安徽东方金桥农林科技股份有限公司 | Method for improving root cutting seedling raising of Tilia miqueliana Maxim |
| CN113498687A (en) * | 2021-03-22 | 2021-10-15 | 浙江农林大学 | Cultivation method of grafting stock of magnolia denudata |
| CN113812297A (en) * | 2021-09-14 | 2021-12-21 | 广西南亚热带农业科学研究所 | Rapid breeding method of black raspberries |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111919608A (en) * | 2020-08-05 | 2020-11-13 | 安徽东方金桥农林科技股份有限公司 | Method for improving root cutting seedling raising of Tilia miqueliana Maxim |
| CN113498687A (en) * | 2021-03-22 | 2021-10-15 | 浙江农林大学 | Cultivation method of grafting stock of magnolia denudata |
| CN113812297A (en) * | 2021-09-14 | 2021-12-21 | 广西南亚热带农业科学研究所 | Rapid breeding method of black raspberries |
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Application publication date: 20200717 |
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