CN101142893B - Water-planting method for arabidopsis thaliana - Google Patents
Water-planting method for arabidopsis thaliana Download PDFInfo
- Publication number
- CN101142893B CN101142893B CN2007100715625A CN200710071562A CN101142893B CN 101142893 B CN101142893 B CN 101142893B CN 2007100715625 A CN2007100715625 A CN 2007100715625A CN 200710071562 A CN200710071562 A CN 200710071562A CN 101142893 B CN101142893 B CN 101142893B
- Authority
- CN
- China
- Prior art keywords
- seedling
- culture tube
- vermiculite
- arabidopsis
- nutrient solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Hydroponics (AREA)
Abstract
The invention discloses a water-culture method for the mouseearcress and comprises the procedures that 1) sprouting of the seed-the seed of the mouseearcress after the yarovization is cultivated in a cultivation room to be a young seedling; 2) a cultivation tool is made; 3) transplanting the seedling-the seedling obtained in the procedure 1) is transplanted to a cultivation pipe obtained in the procedure 2) in the cultivation room and the root of the young seedling contacts the moisture vermiculite; after the young seedling is cultivated for eight to nine days to be a small seedling; 4) culture of seedling-the vermiculite inside the cultivation pipe is primarily removed; Hoagland nutrition solution with one fourth concentration is arranged inside a water tank that is used as the water-culture device; then the cultivation pipe is vertically arranged inside the water tank arranged inside the cultivation room, thereby the root of the small seedling is immersed in the Hoagland nutrition solution. Adopting the method of the invention to cultivate the mouseearcress can effectively improve the survival rate and is convenient and simple to operate.
Description
Technical field
The present invention relates to a kind of ciltivating process of the method for cultivating arabidopsis, particularly arabidopsis.
Background technology
Arabidopsis is that first finishes the higher plant of full gene group sequencing in the world, utilizes its research of carrying out the plant function genomics as model plant to carry out like a raging firely in countries in the world.Utilize arabidopsis to carry out botany research, can provide the basic test material for information, discovery and the clone's important gene of excavating multiple metabolic pathway in the plant corpus.At present, the training method of arabidopsis mainly contains the mixotrophism medium and the nutrient solution cultivation dual mode of peat and vermiculite.Wherein, water planting is the common a kind of planting type in laboratory; But the ciltivating process of report exists certain deficiency both at home and abroad, and its disadvantage is easy microbiological contamination or withered phenomenon occurs.
Summary of the invention
The technical problem to be solved in the present invention provides the ciltivating process of a kind of survival rate that can effectively improve arabidopsis and arabidopsis easy and simple to handle.
In order to solve the problems of the technologies described above, the invention provides a kind of ciltivating process of arabidopsis, may further comprise the steps:
1), the sprouting of seed:
The seed of arabidopsis gained that will be after vernalization becomes seedling cultivating indoor cultivation, grows to 0.9～1.1cm until root;
2), make the cultivation instrument:
Select for use pipe that top and bottom be equipped with opening as culture tube, culture tube is fixed in the container of the Huo Gelan nutrient solution that fills 1/4 concentration; Then the vermiculite after the sterilization treatment is put into culture tube, the placement of vermiculite highly accounts for 80%～100% of culture tube height, and vermiculite becomes moistening vermiculite after absorbing nutrient solution, and the height of culture tube is 2.6～3.0cm, and the internal diameter of culture tube is 9～11mm;
3), transplant seedlings:
In culturing room, the seedling of step 1) gained is put into step 2) culture tube of gained, make the moistening vermiculite of root contact of seedling; Cultivate after 8～9 days, get seedling; Described cultivation indoor temperature is that 20～28 ℃, humidity are 60%～80%; Light source adopts the biological effect lamp of being made up of at interval white light and red globe lamp, and the opening time of light source is 10-12h;
4), grow seedlings:
Remove the vermiculite in the culture tube earlier, in as the tank of hydroponic device, place the Huo Gelan nutrient solution of 1/4 concentration; Again above-mentioned culture tube is vertically put into the tank that is arranged in culturing room, the root system of seedling is immersed in the Huo Gelan nutrient solution; Cultivating indoor temperature and be 20～28 ℃, humidity is 60%～80%, and light source adopts the biological effect lamp of being made up of at interval white light and red globe lamp, and the opening time of light source is 10-12h.
Improvement as the ciltivating process of arabidopsis of the present invention: in the step 4), change the Huo Gelan nutrient solution weekly 1 time, and in the Huo Gelan nutrient solution, inculcate oxygen; Step 2) in, the bottom opening of culture tube and the bottom of container are at a distance of 0.8～1.2cm, and container is selected 96 hole centrifuge tube boxes for use, and culture tube is converted by the eppendorf pipe; In the step 3), the seedling of step 1) gained is put into step 2 with the tip tweezers) culture tube of gained.
In the present invention, the Huo Gelan nutrient solution of 1/4 concentration refers to: with 1: 3 volume ratio mix back formed with water the Huo Gelan nutrient solution of routine.The seedling raise period of step 4) is decided on actual specific requirement; Promptly when the root system of arabidopsis reaches actual required length, just can finish to grow seedlings.
The ciltivating process of arabidopsis of the present invention is in step 2) in, culture tube is positioned in the 96 hole centrifuge tube boxes, be applicable to seedling growth test in enormous quantities, and the humidity that helps keeping suitable arabidopsis seedling to survive.Adopting the eppendorf pipe to repack culture tube into can use repeatedly, therefore can reduce production costs; And the eppendorf pipe is cheap for the common consumptive material in laboratory.In step 3), the arabidopsis seedling is placed in the culture tube that vermiculite is housed cultivates, can reduce the microbiological contamination rate of this process; In the present invention, microbiological contamination rate is almost nil.The overall process of transplanting seedlings does not directly contact hand, thereby can not produce the seedling death that manual operation improper (adopting sponge wrapping to transplant as some method) causes yet.As long as can all can survive, thereby improve survival rate greatly by method cultivation of the present invention the normal seedling that germinates in culture dish the inside.
The ciltivating process of arabidopsis of the present invention, have easy and simple to handle, with low cost, be difficult for microbiological contamination, the high advantage of survival rate.
Description of drawings
Below in conjunction with accompanying drawing the specific embodiment of the present invention is described in further detail.
Fig. 1 is the step 3) of the present invention state cross-sectional schematic of transplanting seedlings;
Fig. 2 is the step 4) of the present invention state cross-sectional schematic of growing seedlings.
Embodiment
A kind of ciltivating process of arabidopsis, carry out following steps successively:
1), the sprouting of seed:
The arabidopsis gained seed 10min of alcohol disinfecting with 75% after vernalization, the back is seeded in subsequently on the sterilization MS medium that is arranged in culture dish and (removes the MS medium of sucrose, see Table 1) with sterile water washing 3 times, regulate pH to 5.7, agarose concentration is 0.55%.After sowing finishes, seal film with Parafilm culture dish is wrapped up a circle, in order to avoid water evaporates and avoid microbiological contamination.In culturing room, placed subsequently 4～5 days, when root is about 1cm seedling 4, can be used for transplanting seedlings.Condition of culture is 20～28 ℃ of temperature, is 60%～80% with room humidifier humidification to humidity; Light source adopts the biological effect lamp of being made up of at interval white light and red globe lamp, and the opening time of light source (being the set time on daytime) is 8h, and seed is apart from light source 25～30cm (the special-purpose culturing rack of also available commercially available arabidopsis).
Table 1 removes sucrose MS culture medium prescription
| Composition | Content (mg/L) | Composition | Content (mg/L) |
| NH 4 | 1650 | KI | 0.83 |
| KNO 3 | 1900 | H 3BO 3 | 6.2 |
| CaCl 2 | 440 | MnSO 4·7H 2O | 22.3 |
| MgSO 4·7H 2O | 370 | ZnSO 4·2H 2O | 8.6 |
| Composition | Content (mg/L) | Composition | Content (mg/L) |
| KH 2PO 4 | 170 | Na 2MoO 4·2H 2O | 0.25 |
| FeSO 4·7H 2O | 27.8 | CuSO 4·5H 2O | 0.025 |
| Na 2EDTA·2H 2O | 37.3 | CoCl 2·6H 2O | 0.025 |
2), make the cultivation instrument:
Select for use the eppendorf pipe (commercially available) of 1.5ml to transform, thereby make required culture tube 1, specific as follows:
With scissors the eppendorf pipe lid of 1.5ml is cut removal; And shear at 7mm place, distance bottom, remove the hypomere part; Thereby forming the high 2.8cm of being, a caliber (internal diameter) is 10mm, and external diameter is 12mm, and the bottom opening diameter is about the culture tube 1 (as shown in Figure 1) of 8mm.
Select for use 96 hole centrifuge tube boxes 2 (commercially available) as container, 96 hole centrifuge tube boxes, 2 long 17cm, wide 9.5cm, each aperture are 12mm.
The eppendorf of each reincarnate is managed every hole that (being culture tube 1) puts into 96 hole centrifuge tube boxes 2 successively, and the bottom of the bottom opening of eppendorf pipe and 96 hole centrifuge tube boxes 2 is at a distance of about 1.0cm at this moment.Toward the Huo Gelan nutrient solution (pH 5.8-6.0) of 96 hole centrifuge tube boxes, 2 addings, 1/4 concentration, liquid level is 2cm, and the bottom that promptly guarantees the eppendorf pipe is by the submergence of Huo Gelan nutrient solution.
Subsequently, add the vermiculite (commercially available) of prior sterilization treatment in each eppendorf pipe, vermiculite can select for use particle size to be about 1mm
3/ grain, the placement height and the eppendorf pipe face of vermiculite maintain an equal level.The vermiculite water absorbing capacity is very quick, in case be arranged in the nutrient solution that the vermiculite of eppendorf pipe bottom contacts 96 hole centrifuge tube boxes 2, the homogeneous tube vermiculite just promptly keeps moisture state, thereby forms moistening vermiculite.Rely on the vermiculite frictional force each other and the characteristic of vermiculite suction back rapid expanding, vermiculite can not managed bottom opening from eppendorf and be spilt.
3), transplant seedlings:
In culturing room, with the tip tweezers seedling in the culture dish of step 1) gained 4 is pressed from both sides out gently, put into step 2) eppendorf of gained pipe (being culture tube 1), make the moistening vermiculite of root contact of seedling 4 get final product (not needing the root of seedling 4 is buried in the vermiculite).In each eppendorf pipe, the strain of transplanting seedlings.
Transplant seedlings finish after, gently the cover last 96 hole centrifuge tube boxes 2 lid, to prevent the nutrient solution excessive vaporization in the box; Certainly, the air in culturing room's this moment still can freely enter in the box.After 1～2 day, remove lid gradually, make seedling can slowly adapt to the environment of culturing room; Cultivate again and get seedling 5 about 7 days.Cultivating indoor temperature and be 20～28 ℃, humidity is 60%～80%, and light source adopts the biological effect lamp of being made up of at interval white light and red globe lamp, and it is 10-12h that the time on daytime is set, and plant is apart from light source 25～30cm.
The seedling 5 that observation post gets can find that its root system develops, and has exceeded the length of eppendorf pipe downwards; And the coverage rate of its blade has surpassed the mouth of pipe (being the open top of eppendorf pipe) of eppendorf pipe.This moment 96 hole centrifuge tube boxes 2 the space, seem narrow and small with respect to the further growth of arabidopsis, therefore need change arabidopsis over to cultivation system, be beneficial to its further growth and satisfy different scientific research demands.
4), grow seedlings:
A, removal vermiculite:
Since many scientific research processes to as if the root system of arabidopsis, therefore need obtain single root system to the vermiculite wash-out.At this moment, the eppendorf pipe is taken out from 96 hole centrifuge tube boxes 2, earlier, extract toothpick again, make vermiculite loosening to some extent with toothpick (the head point inserts easily, and wooden can the not hinder root) jacking of keeping to the side from the bottom of eppendorf pipe; Then clamping the eppendorf pipe with tweezers puts into the beaker that distilled water is housed, and makes eppendorf pipe dandle in water then, and vermiculite promptly skids off from the opening part of eppendorf pipe bottom.
The step of above-mentioned removal vermiculite should be as far as possible clean indoor carrying out.The method of above-mentioned removal vermiculite can guarantee the integrality of plant root, thereby plant can not occur growth retardation because of transplanting seedlings; And the method is simple to operate.
At this moment, because the part stretching, extension of the blade face of arabidopsis seedling 5, the overground part branch of seedling 5 crawls on the eppendorf pipe.This moment, the root of seedling 5 will freely be erected in eppendorf pipe central authorities, has played the supporting role to seedling 5 if the eppendorf pipe is vertically put to water.Because the root system of arabidopsis is less, enough its growths in space in the eppendorf pipe, thereby can not have a negative impact to plant.
B, making hydroponic device:
Available PVC plate be processed into the degree of depth greater than the tank 3 (end uncovered is arranged) of 10cm as hydroponic device, the length of tank 3 and widely can decide according to the quantity of cultivating seedling.Add a cover a slice and be slightly larger than the plastic foam plate 6 (thickness 1-1.5cm) of tank 3 frames on tank 3, the punching footpath is the circular hole of 12mm (just in time being the outer warp of eppendorf pipe) on plastic foam plate 6, and distance between borehole is 5-8cm.
C, formally grow seedlings:
Levelness with horizon rule adjustment tank 3, the eppendorf pipe (being culture tube 1) that has arabidopsis seedling 5 behind the above-mentioned removal vermiculite is inserted in plastic foam plate 6 circular holes, and the plate face that just makes eppendorf mouth of pipe edge projection contact plastic foam plate 6 is advisable.The Huo Gelan nutrient solution of 1/4 concentration is added in the tank 3, keep liquid level and tank mouth to maintain an equal level, again plastic foam plate 6 is covered on tank 3, the root system of seedling 5 all is immersed in the nutrient solution.
In order to increase the oxygen supply of root system, available goldfish jar ventilation pump air feed.Gas is divided into several, and directly inserting plastic foam plate 6 with the minute hand head at the diverse location place with respect to tank 3 can feed oxygen in the nutrient solution.This kind ventilating mode is easy and air feed is even.Nutrient solution is changed weekly 1 time.
Above-mentioned formal seedling raising process carries out in culturing room, and cultivating indoor temperature and be 20～28 ℃, humidity is 60%～80%.Light source adopts the biological effect lamp of being made up of at interval white light and red globe lamp, and it is 10-12h that the time on daytime is set.Plant is apart from light source 25～30cm.
Incubation time can be decided the requirement of root length according to reality, generally cultivates 2 all backs root lengths and can reach 6-10cm (some difference between the arabidopsis kind).
At last, it is also to be noted that what more than enumerate only is a specific embodiment of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention all should be thought protection scope of the present invention.
Claims (7)
1. the ciltivating process of an arabidopsis is characterized in that may further comprise the steps:
1), the sprouting of seed:
The seed of arabidopsis gained that will be after vernalization becomes seedling cultivating indoor cultivation, grows to 0.9～1.1cm until root;
2), make the cultivation instrument:
Select for use pipe that top and bottom be equipped with opening as culture tube, culture tube is fixed in the container of the Huo Gelan nutrient solution that fills 1/4 concentration; Then the vermiculite after the sterilization treatment is put into culture tube, the placement of vermiculite highly accounts for 80%～100% of culture tube height, and vermiculite becomes moistening vermiculite after absorbing nutrient solution, and the height of culture tube is 2.6～3.0cm, and the internal diameter of culture tube is 9～11mm;
3), transplant seedlings:
In culturing room, the seedling of step 1) gained is put into step 2) culture tube of gained, make the moistening vermiculite of root contact of seedling; Cultivate after 8～9 days, get seedling; Described cultivation indoor temperature is that 20～28 ℃, humidity are 60%～80%, and light source adopts the biological effect lamp of being made up of at interval white light and red globe lamp, and the opening time of light source is 10-12h;
4), grow seedlings:
Remove the vermiculite in the culture tube earlier, in as the tank of hydroponic device, place the Huo Gelan nutrient solution of 1/4 concentration; Again above-mentioned culture tube is vertically put into the tank that is arranged in culturing room, the root system of seedling is immersed in the Huo Gelan nutrient solution; Cultivating indoor temperature and be 20～28 ℃, humidity is 60%～80%, and light source adopts the biological effect lamp of being made up of at interval white light and red globe lamp, and the opening time of light source is 10-12h.
2. the ciltivating process of arabidopsis according to claim 1 is characterized in that: change the Huo Gelan nutrient solution in the described step 4) weekly 1 time.
3. the ciltivating process of arabidopsis according to claim 2 is characterized in that: inculcate oxygen in the described step 4) in the Huo Gelan nutrient solution.
4. the ciltivating process of arabidopsis according to claim 3 is characterized in that: the bottom of the bottom opening of culture tube and container 0.8～1.2cm apart described step 2).
5. the ciltivating process of arabidopsis according to claim 4, it is characterized in that: the container described step 2) is 96 hole centrifuge tube boxes.
6. the ciltivating process of arabidopsis according to claim 5, it is characterized in that: the culture tube described step 2) is converted by the eppendorf pipe.
7. the ciltivating process of arabidopsis according to claim 6 is characterized in that: in the described step 3), with the tip tweezers seedling of step 1) gained is put into step 2) culture tube of gained.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100715625A CN101142893B (en) | 2007-10-09 | 2007-10-09 | Water-planting method for arabidopsis thaliana |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100715625A CN101142893B (en) | 2007-10-09 | 2007-10-09 | Water-planting method for arabidopsis thaliana |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101142893A CN101142893A (en) | 2008-03-19 |
| CN101142893B true CN101142893B (en) | 2010-11-17 |
Family
ID=39205766
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007100715625A Expired - Fee Related CN101142893B (en) | 2007-10-09 | 2007-10-09 | Water-planting method for arabidopsis thaliana |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101142893B (en) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101731135B (en) * | 2008-11-26 | 2011-08-24 | 中国科学院大连化学物理研究所 | Device for directly seeding and culturing arabidopsis thaliana in water and application thereof |
| CN101904294B (en) * | 2010-07-15 | 2012-03-21 | 中国科学院南京土壤研究所 | Device for screening and culturing of transgenic arabidopsis seedlings in situ and application thereof |
| CN102265780A (en) * | 2011-05-15 | 2011-12-07 | 浙江大学 | Simple seedling raising method of Arabidopsis seeds and used device |
| CN102812891A (en) * | 2012-06-21 | 2012-12-12 | 徐州红高粱生态园有限公司 | Three-dimensional planting bradysia odoriphaga prevention and treatment method |
| CN103004569A (en) * | 2012-11-27 | 2013-04-03 | 天津滨海国际花卉科技园区股份有限公司 | Hydroponic planting device and method for planting plants by using same |
| CN104365361B (en) * | 2014-11-20 | 2016-03-02 | 中国农业科学院农业环境与可持续发展研究所 | The nursery of a kind of matrix institute wash method for root and application thereof |
| CN104855259B (en) * | 2015-04-30 | 2017-05-10 | 郑州大学 | Sinapsis alba seedling water planting method |
| CN105210837B (en) * | 2015-10-20 | 2018-04-06 | 中国农业科学院北京畜牧兽医研究所 | The method for carrying out rhizobium dross effect assessment using the sterile hydroponic device of plant |
| CN105272464A (en) * | 2015-10-28 | 2016-01-27 | 安徽科技学院 | Hoagland nitrogen-deficiency plant nutrition liquid reagent kit |
| RU2612319C1 (en) * | 2015-12-17 | 2017-03-06 | Общество с ограниченной ответственностью "ШеньжоуСпейсБайтекнолоджиГруп" | Method of growing the plants with using metal nanoparticles and substratum for its implementation |
| CN105706873B (en) * | 2016-02-03 | 2019-03-08 | 青岛农业大学 | The engrafting method of one mode arabidopsis |
| CN105830888B (en) * | 2016-03-30 | 2018-08-07 | 东北林业大学 | A kind of method of stinkgrass flower live streaming water planting |
| CN105794622A (en) * | 2016-04-20 | 2016-07-27 | 中国农业科学院作物科学研究所 | Plant water culture method and special device thereof |
| CN106465675A (en) * | 2016-08-31 | 2017-03-01 | 黄福萍 | A kind of ciltivating process of arabidopsiss |
| CN106386432A (en) * | 2016-08-31 | 2017-02-15 | 黄福萍 | Hydroponics method of model plants |
| CN106386433A (en) * | 2016-08-31 | 2017-02-15 | 黄福萍 | Method for cultivation of Arabidopsis |
| CN106386431A (en) * | 2016-08-31 | 2017-02-15 | 黄福萍 | Bacteriostatic hydroponics method of Arabidopsis |
| CN106376446A (en) * | 2016-08-31 | 2017-02-08 | 黄福萍 | Method for prolonging water planting time of arabidopsis thaliana |
| CN107637491A (en) * | 2017-09-22 | 2018-01-30 | 浙江省林业科学研究院 | Suitable for physiology and the arabidopsis cultural method and culture apparatus special of genetic research |
| CN109362392B (en) * | 2018-12-26 | 2020-09-18 | 厦门大学 | Illumination device capable of effectively promoting vegetative growth of arabidopsis thaliana |
| CN114982614A (en) * | 2022-06-13 | 2022-09-02 | 广东省农业科学院农业生物基因研究中心 | Method for water culture of epimedium brevicornum |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1902997A (en) * | 2006-08-02 | 2007-01-31 | 中国科学院植物研究所 | Method for cultivation of arabidopsis |
-
2007
- 2007-10-09 CN CN2007100715625A patent/CN101142893B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1902997A (en) * | 2006-08-02 | 2007-01-31 | 中国科学院植物研究所 | Method for cultivation of arabidopsis |
Non-Patent Citations (4)
| Title |
|---|
| 刘峰,施卫明.拟南芥室内水培方法的改进.土壤38 1.2006,38(1),102-105. |
| 刘峰,施卫明.拟南芥室内水培方法的改进.土壤38 1.2006,38(1),102-105. * |
| 钟海秀,戴绍军,阎秀峰.水培拟南芥中芥子油苷组成与含量的初步分析.黑龙江大学自然科学学报24 3.2007,24(3),321-323,327. |
| 钟海秀,戴绍军,阎秀峰.水培拟南芥中芥子油苷组成与含量的初步分析.黑龙江大学自然科学学报24 3.2007,24(3),321-323,327. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101142893A (en) | 2008-03-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101142893B (en) | Water-planting method for arabidopsis thaliana | |
| CN101731135B (en) | Device for directly seeding and culturing arabidopsis thaliana in water and application thereof | |
| AU2009227478B2 (en) | Container for culture of plant, method for culture of plant and method of production of cutting seedling | |
| CN105104152A (en) | Water planting device and water planting method for arabidopsis thaliana | |
| CN207284383U (en) | A kind of forestry sapling breeding apparatus | |
| CN107018896B (en) | A kind of method of facility cuttage tilia miqueliana | |
| CN102090329A (en) | Method for transplanting Damascus rose tissue culture seedling | |
| CN112189566B (en) | Rapid breeding method of cherry seedlings for stocks | |
| CN102273405B (en) | Cultivating method of Hosta plantaginea Gold Standard | |
| CN103314862B (en) | A kind of method of efficient acquisition Chunlan detoxification seedling | |
| CN104770173A (en) | High-efficiency seedling cultivation method for promoting premature tillering of sugarcane tissue cultured seedlings | |
| CN103843651A (en) | Facility semi-water planting culture device and facility semi-water planting culture method for root and rhizome medicinal plants | |
| CN107047272A (en) | Crops seedling stage nutritional test method and device | |
| CN101855969A (en) | Grafting root-planting method for cotton | |
| CN111149674A (en) | Culture method for improving transplanting survival rate of plant tissue culture seedlings | |
| CN208129133U (en) | A kind of novel monkey camphor tree cuttage and seedling culture case | |
| CN101112173B (en) | Tissue culture propagating method for heavy metal super-enriched thlaspi caerulescens | |
| CN104920219A (en) | Dendrobium devonianum rapid propagation seedling survival culture medium series and tissue culture method | |
| CN111034557B (en) | Seedling for indoor inoculation of barley yellow mosaic disease and culture method of seedling after inoculation | |
| CN103535280A (en) | Water oak tissue culture breeding method | |
| Cho et al. | Several factors affecting mass production of Microlepia strigosa (Thunb.) C. Presl sporophytes | |
| WO2020186705A1 (en) | Root control cultivation apparatus | |
| CN108782205B (en) | Cultivation method for strong arabidopsis seedlings | |
| CN113197077A (en) | Floating hardening method for potato test-tube plantlets | |
| CN206699017U (en) | The device of crops seedling stage nutritional test method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20101117 Termination date: 20111009 |