CN101855969A - Grafting root-planting method for cotton - Google Patents
Grafting root-planting method for cotton Download PDFInfo
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- CN101855969A CN101855969A CN201010190429A CN201010190429A CN101855969A CN 101855969 A CN101855969 A CN 101855969A CN 201010190429 A CN201010190429 A CN 201010190429A CN 201010190429 A CN201010190429 A CN 201010190429A CN 101855969 A CN101855969 A CN 101855969A
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Abstract
The invention discloses a grafting root-planting method for cotton. In the method, cotton seedlings, which have 2 to 3 leaves, complete root systems and consistent sizes, serve as grafting seedlings; the part above the cotyledon node of one cotton seedling (rootstock) is removed and the remaining part is directly grafted to the lower hypocotyl of another complete cotton seedling (scion) to ensure that the grafted cotton seedling has two complete root systems (containing two lower hypocotyls) and one shared scion, wherein attention is paid to heat and moisture preservation in a recovering stage and grafting survival and success rates are over 95 percent. The root-planting method has the advantages of simple operation, capacity of keeping the completeness of the root system and achievement of consistent growth of the two grafted root systems. Apart from important research and application values, the method can be used for carrying out the research for a drought stress, a salt stress and the like for taproot system plants, such as the cotton and the like, and also can be used for ornamental plants, such as flowers and the like to improve an ornamental value and a commercial value through rooting planting.
Description
Technical field
The present invention relates to a kind of root-planting method of cotton seedling, be specially grafting, realize the concrete grammar of root division by cotton seedling.
Background technology
How Blackman in 1985 and Davies control stomatal movement in order to have studied soil moisture, at first design and utilized root-planting method in corn.Subsequently, root-planting method sees various crop such as sunflower, apple, oranges and tangerines, grape, cotton again.Root-planting method has been widely used in a plurality of research fields such as drought tolerance in plants, salt tolerant and Nutrient Absorption at present, and meanwhile, root-planting method also is used for some flowers and ornamental plants, improves its ornamental value by root division.
Although root-planting method is constantly perfect over nearly more than 20 years, the root-planting method that adopts all is directly root system to be divided into two parts or many parts at present, and this accomplishes than being easier to for some system of fibrous root plants; But for system of taproot plant, need before root division, cut main root earlier, and then lateral root is divided into two parts or many parts, be planted in the special container as cotton.This traditional root-planting method not only needs special device, and survival rate is low, and because will cut main root, the situation of simulating plant normal growth growth will be had a greatly reduced quality as its accuracy of research system fully.Therefore, set up a kind of main root that neither destroys, the novel root-planting method that can improve the cotton shoot survival percent of root division again is very necessary.
Summary of the invention
The objective of the invention is to solve the deficiency of traditional root-planting method, the root-planting method of easy, the high-servival rate of a kind of system of taproot plant that is fit to comprise cotton is provided.The both sides root system was complete after this method can guarantee root division, survive with success rate more than 95%.
The technical solution adopted in the present invention is: a kind of cotton grafting root-planting method may further comprise the steps:
(1) preparation of cotton seedling
Choose and enrich full cotton seeds, be seeded in the river sand of sterilization (husky water quality ratio is 6: 1), put (22～32 ℃) germination and emergence in the greenhouse; After cotton seedling grows rough leaf, give suitably arid and handled (water by control and make cotton leaf that slight the wilting be taken place) 5～7 days, with hardening and promote root growth and development; After treating that cotton seedling grows 2～3 true leaves, dig out cotton seedling, select the cotton seedling of complete, the of the same size stalwartness of root system, a part is as the scion strain, and a part is as the stock strain.
(2) grafting and root division
Basic skills is: it adopts above-mentionedly has 2～3 true leaves, complete, the of the same size cotton seedling of root system as grafting, a cotton seedling (stock strain) cotyledonary node is removed with the upper part, the direct grafting of remainder is to the hypocotyl of another complete cotton seedling (scion strain), thereby form a complete cotton seedling, have two complete root systems (containing two hypocotyls), a shared scion.In order to narrate conveniently, not only calling the scion strain, a cotton seedling that is used as stock is called the stock strain as scion but also as the complete cotton seedling of stock.Idiographic flow is:
On the hypocotyl at 2cm place under the scion strain cotyledonary node, with the sharp cutter a kerf of cutting sth. askew from bottom to top, otch becomes 20～30 ° of angles with hypocotyl, and notch depth is a hypocotyl cross section 2/3; To remove with top as the cotton young plant leaf segment of stock strain, under cotyledonary node on the hypocotyl at 2cm place, the capable wedge of tongue that the otch of and scion of cutting sth. askew from bottom to top matches inserts in the otch of scion strain, wraps up fixedly stock and scion junction (seeing accompanying drawing 1) with sealing film.
(3) root division after the grafting is cultivated
The good plant of step (2) grafting is put into the pot for growing seedlings that nutrient solution or seedling medium are housed, as cultivating, changed one time of nutrition liquid in per two days with nutrient solution; The plant that grafting is good is put into illumination box, illumination in 14 hours, and 10 hours dark, temperature are 30 ℃, humidity 〉=80% delays and finishes root division after seedling 3-5 days, promptly can be used for research or his usefulness.
Root-planting method of the present invention is compared with conventional method, has the following advantages:
(1) root system is complete, particularly kept main root, can simulate the normal upgrowth situation of cotton more accurately, grafting root-planting place and root system have certain distance, relatively be fit to do accurate water culture experiment, can keep separate between different disposal, the root growth situation of plant both sides came into plain view before test was handled, be beneficial to the branch offspring of choosing both sides root growth unanimity, reduce the error of test.
(2) simple to operate, survival rate is high, the root division survival rate of this method and success rate are significantly increased than traditional root division survival rate (about 80%) all more than 95%;
(3) do not need special root division device, simple and practical.
The present invention is not only applicable to cotton, is applicable to that other system of taproot plants carry out the research of drought stress, salt stress aspect yet.The present invention not only has important researching value, also can be used for flowers and other ornamental plantss, improves ornamental value and commercial value by root division, has certain business development prospect.
Description of drawings
Accompanying drawing 1 is the schematic diagram of cotton grafting root-planting method.
Embodiment
Embodiment 1:
Get the plastic basin of a diameter 50cm, high 30cm, fill the wet river sand (husky water quality ratio is 6: 1) of sterilization; Grind No. 21 100 in lint seed with enriching full Shandong cotton, uniform broadcasting in river sand, seed level 2cm; The plastic basin opening part covers with transparent plastic film, puts (22～32 ℃) germination and emergence in the greenhouse; Take plastic film behind the Qi Miao off, water by control and carry out slight arid hardening to the slight wilting of blade generation; After treating that cotton seedling grows second true leaf, dig out cotton seedling, select the cotton seedling of complete, the of the same size stalwartness of root system, a part is as the scion strain, and a part is as the stock strain.
2cm place under scion strain hypocotyl cotyledonary node, with the sharp cutter a kerf of cutting sth. askew from bottom to top, otch becomes 20-30 ° of angle with hypocotyl, and notch depth is a hypocotyl cross section 2/3; Equally, 2cm place under stock strain hypocotyl cotyledonary node, the capable wedge of tongue that matches with the scion otch of cutting sth. askew from bottom to top inserts in the otch of scion, with sealing film wrapping fixedly stock and scion junction; The plant that grafting is good is put into the preprepared pot for growing seedlings, and nutrient solution is housed in the pot for growing seedlings, and nutrient solution changed once in per two days; The plant that grafting is good is put into illumination box, illumination in 14 hours, and 10 hours dark, 30 ℃ of temperature, humidity 80%, slow seedling 5 days survives, success rate reaches 96%, both sides root growth unanimity.
Embodiment 2:
Get the plastic basin of a long 80cm, wide 50cm, high 20cm, fill the wet river sand (husky water quality ratio is 6: 1) of sterilization; Grind No. 28 200 in lint seed with enriching full Shandong cotton, uniform broadcasting in river sand, seed level 2cm; The plastic basin opening part covers with transparent plastic film, puts (22～32 ℃) germination and emergence in the greenhouse; Take plastic film behind the Qi Miao off, water by control and carry out slight arid hardening to the slight wilting of blade generation; After treating that cotton seedling grows second true leaf, dig out cotton seedling, select the cotton seedling of complete, the of the same size stalwartness of root system, a part is as the scion strain, and a part is as the stock strain.
2cm place under scion strain hypocotyl cotyledonary node, with the sharp cutter a kerf of cutting sth. askew from bottom to top, otch becomes 20-30 ° of angle with hypocotyl, and notch depth is a hypocotyl cross section 2/3; Equally, 2cm place under stock strain hypocotyl cotyledonary node, the capable wedge of tongue that matches with the scion otch of cutting sth. askew from bottom to top inserts in the otch of scion, with sealing film wrapping fixedly stock and scion junction; The plant that grafting is good is put into the preprepared pot for growing seedlings, and seedling medium is housed in the pot for growing seedlings; The plant that grafting is good is put into illumination box, illumination in 14 hours, and 10 hours dark, 30 ℃ of temperature, humidity 80%, slow seedling 5 days survives, success rate reaches 95%, both sides root growth unanimity.
Claims (4)
1. cotton grafting root-planting method, it is characterized in that, it adopts has 2～3 true leaves, complete, the of the same size cotton seedling of root system as grafting, a cotton young plant leaf segment is removed with the upper part, the direct grafting of remainder makes the cotton seedling after the grafting have two complete root systems, a shared scion to the hypocotyl of another complete cotton seedling; The cotton seedling that described cotyledonary node removes with the upper part is the stock strain, and another complete cotton seedling is the scion strain, and described two complete root systems contain two hypocotyls.
2. a kind of cotton grafting root-planting method as claimed in claim 1 is characterized in that, may further comprise the steps:
(1) preparation of cotton seedling: cotton seeds is seeded in the river sand, puts germination and emergence in the greenhouse; After cotton seedling grows rough leaf, give suitably arid and handled 5～7 days, with hardening and promote root growth and development; After treating that cotton seedling grows 2～3 true leaves, dig out cotton seedling, select the cotton seedling of complete, the of the same size stalwartness of root system, a part is as the scion strain, and a part is as the stock strain, and the husky water quality ratio of described river sand is 6: 1;
(2) grafting and root division: on the hypocotyl at 2cm place under the scion strain cotyledonary node, a kerf of cutting sth. askew from bottom to top, otch become 20～30 ° of angles with hypocotyl, and notch depth is a hypocotyl cross section 2/3; To remove with top as the cotton young plant leaf segment of stock strain, under cotyledonary node on the hypocotyl at 2cm place, the capable wedge of tongue that the otch of cut sth. askew from bottom to top and scion matches inserts in the otch of scion, wraps up fixedly stock and scion junction with sealing film;
(3) root division after the grafting is cultivated: the good plant of step (2) grafting is put into the pot for growing seedlings that nutrient solution or seedling medium are housed, move in the illumination box, illumination in 14 hours, 10 hours dark, 30 ℃ of temperature are delayed under the condition of humidity 〉=80% and are finished root division after seedling 3-5 days.
3. a kind of cotton grafting root-planting method as claimed in claim 2 is characterized in that, in described step (3) pot for growing seedlings nutrient solution is housed, and described nutrient solution changed once in per two days.
4. a kind of cotton grafting root-planting method as claimed in claim 2 is characterized in that, described step (1) cotton seeds is for enriching full cotton seeds, and described river sand is the river sand through sterilization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2010101904293A CN101855969B (en) | 2010-06-03 | 2010-06-03 | Grafting root-planting method for cotton |
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| CN2010101904293A CN101855969B (en) | 2010-06-03 | 2010-06-03 | Grafting root-planting method for cotton |
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| CN101855969A true CN101855969A (en) | 2010-10-13 |
| CN101855969B CN101855969B (en) | 2011-07-20 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107509535A (en) * | 2017-09-01 | 2017-12-26 | 山西省农业科学院棉花研究所 | Dicotyledon seedling and the efficient cleft grafting method of edible tender branch |
| CN109156189A (en) * | 2018-09-27 | 2019-01-08 | 浙江农林大学 | A kind of grafting green cucumber root-planting method |
| CN112088671A (en) * | 2020-10-20 | 2020-12-18 | 莫翌 | Method for cultivating multiple seedlings by using grafting technology |
| CN115250773A (en) * | 2022-07-06 | 2022-11-01 | 建水县浩野农林产业有限公司 | Rapid seedling raising technology for grafting multiple root system cutting seedlings and seeding seedlings of camellia oleifera trees |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE202021104927U1 (en) | 2021-09-13 | 2022-12-19 | Martin Kobus | Root treated plant |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1729746A (en) * | 2005-08-18 | 2006-02-08 | 河北农业大学 | Cotton grafting and fit grafting method |
| CN101491197A (en) * | 2009-02-24 | 2009-07-29 | 广西大学 | Cotton beveling engrafting method |
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2010
- 2010-06-03 CN CN2010101904293A patent/CN101855969B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1729746A (en) * | 2005-08-18 | 2006-02-08 | 河北农业大学 | Cotton grafting and fit grafting method |
| CN101491197A (en) * | 2009-02-24 | 2009-07-29 | 广西大学 | Cotton beveling engrafting method |
Non-Patent Citations (3)
| Title |
|---|
| 《中国农业科学》 20071231 王彦霞等 棉花高效嫁接新方法及其应用 264-270 1-4 第40卷, 第2期 2 * |
| 《作物学报》 20091231 李博等 适用于低钾条件下棉花苗期根冠通讯研究的三种嫁接方法 363-369 1-4 第35卷, 第2期 2 * |
| 《种子科技》 20091231 张香桂等 棉花嫁接技术要点 30-31 1-4 , 第9期 2 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107509535A (en) * | 2017-09-01 | 2017-12-26 | 山西省农业科学院棉花研究所 | Dicotyledon seedling and the efficient cleft grafting method of edible tender branch |
| CN109156189A (en) * | 2018-09-27 | 2019-01-08 | 浙江农林大学 | A kind of grafting green cucumber root-planting method |
| CN112088671A (en) * | 2020-10-20 | 2020-12-18 | 莫翌 | Method for cultivating multiple seedlings by using grafting technology |
| CN115250773A (en) * | 2022-07-06 | 2022-11-01 | 建水县浩野农林产业有限公司 | Rapid seedling raising technology for grafting multiple root system cutting seedlings and seeding seedlings of camellia oleifera trees |
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| CN101855969B (en) | 2011-07-20 |
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