CN103636373A - Method for vegetative propagation of vaccinium plants through fresh sphagnum - Google Patents
Method for vegetative propagation of vaccinium plants through fresh sphagnum Download PDFInfo
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- CN103636373A CN103636373A CN201310536612.8A CN201310536612A CN103636373A CN 103636373 A CN103636373 A CN 103636373A CN 201310536612 A CN201310536612 A CN 201310536612A CN 103636373 A CN103636373 A CN 103636373A
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- 241000736767 Vaccinium Species 0.000 title claims abstract description 37
- 241000736285 Sphagnum Species 0.000 title abstract 5
- 239000000758 substrate Substances 0.000 claims abstract description 10
- 241000196324 Embryophyta Species 0.000 claims abstract description 9
- 239000003337 fertilizer Substances 0.000 claims abstract description 9
- 201000010099 disease Diseases 0.000 claims abstract description 8
- 240000008536 Vaccinium myrtillus Species 0.000 claims abstract description 7
- 235000017537 Vaccinium myrtillus Nutrition 0.000 claims abstract description 7
- LBLSRDDHGILUJH-UHFFFAOYSA-N 4-(1H-indol-2-yl)butanoic acid Chemical compound C1=CC=C2NC(CCCC(=O)O)=CC2=C1 LBLSRDDHGILUJH-UHFFFAOYSA-N 0.000 claims abstract description 5
- 235000015725 bilberry Nutrition 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims abstract description 5
- 239000002985 plastic film Substances 0.000 claims abstract description 5
- 239000011159 matrix material Substances 0.000 claims description 36
- 241001276618 Mayaca fluviatilis Species 0.000 claims description 34
- 210000004185 Liver Anatomy 0.000 claims description 17
- 241000238631 Hexapoda Species 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 230000015572 biosynthetic process Effects 0.000 claims description 8
- 238000005755 formation reaction Methods 0.000 claims description 8
- 239000007921 spray Substances 0.000 claims description 8
- 239000002689 soil Substances 0.000 claims description 5
- 241000233866 Fungi Species 0.000 claims description 4
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- 230000003203 everyday Effects 0.000 claims description 4
- 238000005286 illumination Methods 0.000 claims description 4
- 238000003780 insertion Methods 0.000 claims description 4
- YGGXZTQSGNFKPJ-UHFFFAOYSA-N methyl 2-naphthalen-1-ylacetate Chemical compound C1=CC=C2C(CC(=O)OC)=CC=CC2=C1 YGGXZTQSGNFKPJ-UHFFFAOYSA-N 0.000 claims description 4
- 230000035699 permeability Effects 0.000 claims description 4
- 239000004033 plastic Substances 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 210000004215 spores Anatomy 0.000 claims description 4
- 239000000725 suspension Substances 0.000 claims description 4
- 238000004642 transportation engineering Methods 0.000 claims description 4
- 238000009333 weeding Methods 0.000 claims description 4
- 230000004083 survival Effects 0.000 abstract description 8
- 230000001488 breeding Effects 0.000 abstract description 4
- 241000607479 Yersinia pestis Species 0.000 abstract 1
- 238000010899 nucleation Methods 0.000 abstract 1
- 210000001519 tissues Anatomy 0.000 abstract 1
- 241000195940 Bryophyta Species 0.000 description 5
- 235000013399 edible fruits Nutrition 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 3
- 235000003095 Vaccinium corymbosum Nutrition 0.000 description 2
- 230000000844 anti-bacterial Effects 0.000 description 2
- 239000003899 bactericide agent Substances 0.000 description 2
- 235000021014 blueberries Nutrition 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 241000208421 Ericaceae Species 0.000 description 1
- 208000001491 Myopia Diseases 0.000 description 1
- 229940045997 Vitamin A Drugs 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 229940046009 Vitamin E Drugs 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000004217 heart function Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004379 myopia Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001850 reproductive Effects 0.000 description 1
- 229960003471 retinol Drugs 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 150000003700 vitamin C derivatives Chemical class 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 150000003712 vitamin E derivatives Chemical class 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Abstract
The invention belongs to the technical field of plant vegetative propagation, and particularly relates to a method for vegetative propagation of vaccinium plants through fresh sphagnum. The method is particularly applicable to rapid breeding of some main vaccinium plants. Half-lignified branches or tissue culture seeding stems of bilberry in that very year are selected and soaked in an indolebutyric acid, and then cuttings are arranged at the roots for 2 hours. A fresh sphagnum culture substrate is inoculated with endotrophic mycorrhiza extracted from the vaccinium plants. The cuttings are inserted in the fresh sphagnum culture substrate, and then plastic film mulching is conducted. After cuttage, the temperature is controlled to range from 15 DEGC to 28 DEG C, and relative humidity is controlled to range from 80% to 90%; after the cuttings take roots, liquid complete fertilizer with the concentration of about 0.5% is applied every 20 days. According to the method, the fresh sphagnum is used as the substrate for culture of seedlings of the vaccinium plants in a cuttage mode, the survival rate of the cultured seedlings of the vaccinium plants is increased by means of inoculating the substrate with the mycorrhiza of the vaccinium plants, and therefore it is guaranteed that strong and high-quality seedlings without pest and disease damage are cultured.
Description
Technical field
The invention belongs to plant asexual multiplication technology field, be specifically related to a kind ofly utilize fresh and alive bog moss to carry out vegetative method to genus vaccinium plant, be particularly useful for some main genus vaccinium plants to carry out fast breeding.
Technical background
Genus vaccinium plant is under the jurisdiction of Ericaceae, is generally short and small shrub.This pulp that belongs to most plants fruit is fine and smooth, and pericarp is thin, and seed is minimum, and juice is many, and sweet and sour palatability both can be eaten raw, also can be processed into jam, preserved fruit, fruit drink etc.Its fruit is except containing conventional sugar, organic acid and vitamin C, is also rich in the trace elements such as rare special composition in other fruit such as anthocyan, vitamin E, vitamin A, Cobastab, SOD, protein, fat and abundant potassium, iron, zinc, manganese.At pre-myopia prevention, strengthen aspect cardiac function, anticancer, anti-cardiovascular disease and there is extremely important effect.
Cuttage is as the important means of seedling-wood breeding, simple and easy to do, and can breed out a large amount of breeding nursery stocks within a short period of time, thereby is widely used.One of successful key factor of blueberry cuttage and seedling culture is the selection of matrix.The effect of matrix is except supporting and fixedly plant, the more important thing is that as moisture, air, nutrient, acid-base value etc., and these are all relevant with the physicochemical character of matrix for plant growth provides stable, a suitable root system environment.The particle size of its mesostroma, density, total porosity etc. are important character parameters.At present, the matrix of genus vaccinium plant cuttage mainly contains the types such as vermiculite, the peat composed of rotten mosses, river sand.Yet result of study in the past shows, the success rate of utilizing above-mentioned several matrix to carry out cuttage to genus vaccinium plant is lower, is not ideal cutting medium.Research in recent years finds that bryophyte has the features such as water retention property is strong, air capacity of soils good, corrosion-resistant, temperature changing amplitude is little, bog moss is as the moss extensively distributing in global range, especially extremely common in the Changbaishan area in China northeast, and find that through our previous research the common hobby of genus vaccinium plant is grown in together with bog moss, between them, there is a kind of relation of symbiosis, in bog moss plant corpus, have the endotrophic mycorrhiza identical with genus vaccinium plant.
Also the research and utilization bryophyte having based on this comprises that bog moss carries out cottage propagation as matrix to genus vaccinium plant, yet, not being all liver mosses can be as the desirable matrix of genus vaccinium plant cuttage, mostly adopt non-fresh and alive property bryophyte as matrix, in medium, add various bactericide simultaneously, thereby the reproductive survival rate that makes to grow seedlings is low, and cuttage effect is undesirable.Therefore, utilizing fresh and alive bog moss is that genus vaccinium plant is carried out to vegetative a kind of effective method as genus vaccinium plant cuttage matrix.
Summary of the invention
Present situation for current genus vaccinium plant asexual multiplication technology, the present invention is understanding in depth on the basis of genus vaccinium plant seedling growth characteristic, providing a kind of utilizes fresh and alive bog moss to carry out vegetative method to genus vaccinium plant, the matrix of utilizing fresh and alive bog moss to grow seedlings as genus vaccinium plant cuttage, and improve the survival rate of seedling of genus vaccinium plant by the mycorhiza of inoculation genus vaccinium plant, guarantee to cultivate stalwartness, without the high quality seedling of damage by disease and insect.
To achieve these goals, the present invention realizes as follows: a kind ofly utilize fresh and alive bog moss to carry out vegetative method to genus vaccinium plant, it is characterized in that:
(1) selection of cuttings and processing: choosing then raw semi-lignified, fringe bar sturdy perfectly straight, leaf bud is full, blade is done cuttings without the branch of damage by disease and insect, described fringe bar degree of lignification is low, be beneficial to the formation of callus, and then formation young root, long 6 ~ the 8cm of cuttings, soaks cuttings base portion 2h with the quickly dipped cutting of indolebutyric acid or with methyl α-naphthyl acetate.
(2) preparation of matrix: first the VA Mycorrhizal Fungi spore extracting is mixed with to mycorhiza suspension and is inoculated in the fresh and alive bog moss plant of cultivating in Experimental Establishment on a small quantity in great bilberry, make endotrophic mycorrhiza a large amount of propagation therein; Then, utilize the above-mentioned liver moss having inoculated to be inoculated in fresh and alive bog moss culture matrix as the maternal plant of endotrophic mycorrhiza.Described fresh and alive bog moss matrix, must meet following condition: (1) guarantees that the liver moss of field acquisition is green fresh and alive state; (2) in transportation, utilize the woven bag of good air permeability; (3) guarantee that liver moss water content is more than 50%; (4) fresh and alive liver moss matrix at 30 days with interior use.
(3) prepare in seedbed: cuttage the last week, above-mentioned bog moss matrix is laid into seedbed, can places one deck Polypropylence Sheet, nonwoven below slotting machine or be other planting soils and bare area.
(4) cuttage: by the fresh and alive bog moss matrix of the cuttings inserting step (2) of processing in step (1), water content of substrate remains on 50 ~ 70%, porosity 60 ~ 70%, insertion depth 1 ~ 3cm, with bud and blade, be uncovered and be advisable, carry out plastic mulching and shade.
(5) after cuttage, manage: every day spray cooling, temperature is controlled 15 ~ 28 ℃, humidity is controlled at 80 ~ 90% left and right.After cuttage rooting, remove at noon plastic film at 12 o'clock to 15 o'clock, reduce humidity, increase illumination, and carry out the work of weeding, insect protected; Apply N: P: K is that 13: 26: 13 or N: P: K is the complete value fertilizer of 15: 30: 4, and fertilizer applies with liquid state, at interval of 20d, sprays 1 time.
The described fresh and alive bog moss that utilizes carries out vegetative method to genus vaccinium plant, in order to guarantee to extract the activity of mycorhiza from genus vaccinium plant, in culture matrix, does not add any bactericide.Adopt this method, cuttings starts to take root after cuttage 20d, and after 30 ~ 40d, survival rate is more than 90%, and seedling growth is healthy and strong, breeds efficiency and obviously improves.
Of the present invention producing effect is: compare with genus vaccinium plant asexual multiplication technology in the past, the matrix that this method utilizes fresh and alive bog moss to grow seedlings as genus vaccinium plant cuttage, and improve the survival rate of seedling of genus vaccinium plant by the mycorhiza of inoculation genus vaccinium plant, guarantee to cultivate stalwartness, without the high quality seedling of damage by disease and insect.
Embodiment
Below in conjunction with specific embodiment describe in detail in the present invention different substrates water content and the cuttage degree of depth on the impact of genus vaccinium plant cuttage survival rate (take blueberry as example, in Table 1 and table 2).
Embodiment mono-
(1) selection of cuttings and processing: choosing then raw semi-lignified, fringe bar sturdy perfectly straight, leaf bud is full, blade is done cuttings without the branch of damage by disease and insect, described fringe bar degree of lignification is low, be beneficial to the formation of callus, and then formation young root, the long 6cm of cuttings, soaks cuttings base portion 2h with the quickly dipped cutting of indolebutyric acid or with methyl α-naphthyl acetate.
(2) preparation of matrix: first the VA Mycorrhizal Fungi spore extracting is mixed with to mycorhiza suspension and is inoculated in the fresh and alive bog moss plant of cultivating in Experimental Establishment on a small quantity in great bilberry, make endotrophic mycorrhiza a large amount of propagation therein; Then, utilize the above-mentioned liver moss having inoculated to be inoculated in fresh and alive bog moss culture matrix as the maternal plant of endotrophic mycorrhiza.Described fresh and alive bog moss matrix, must meet following condition: (1) guarantees that the liver moss of field acquisition is green fresh and alive state; (2) in transportation, utilize the woven bag of good air permeability; (3) guarantee that liver moss water content is more than 50%; (4) fresh and alive liver moss matrix at 30 days with interior use.
(3) prepare in seedbed: cuttage the last week, above-mentioned bog moss matrix is laid into seedbed, can places one deck Polypropylence Sheet, nonwoven below slotting machine or be other planting soils and bare area.
(4) cuttage: by the fresh and alive bog moss matrix of the cuttings inserting step (2) of processing in step (1), water content of substrate remains on 50%, porosity 60%, insertion depth 1cm, is uncovered and is advisable with bud and blade, carries out plastic mulching and shades.
(5) after cuttage, manage: every day spray cooling, temperature is controlled 15 ℃, humidity is controlled at 80% left and right.After cuttage rooting, remove at noon plastic film at 12 o'clock to 15 o'clock, reduce humidity, increase illumination, and carry out the work of weeding, insect protected; Apply N: P: K is that 13: 26: 13 or N: P: K is the complete value fertilizer of 15: 30: 4, and fertilizer applies with liquid state, at interval of 20d, sprays 1 time.
Embodiment bis-
(1) selection of cuttings and processing: choosing then raw semi-lignified, fringe bar sturdy perfectly straight, leaf bud is full, blade is done cuttings without the branch of damage by disease and insect, described fringe bar degree of lignification is low, be beneficial to the formation of callus, and then formation young root, the long 8cm of cuttings, soaks cuttings base portion 2h with the quickly dipped cutting of indolebutyric acid or with methyl α-naphthyl acetate.
(2) preparation of matrix: first the VA Mycorrhizal Fungi spore extracting is mixed with to mycorhiza suspension and is inoculated in the fresh and alive bog moss plant of cultivating in Experimental Establishment on a small quantity in great bilberry, make endotrophic mycorrhiza a large amount of propagation therein; Then, utilize the above-mentioned liver moss having inoculated to be inoculated in fresh and alive bog moss culture matrix as the maternal plant of endotrophic mycorrhiza.Described fresh and alive bog moss matrix, must meet following condition: (1) guarantees that the liver moss of field acquisition is green fresh and alive state; (2) in transportation, utilize the woven bag of good air permeability; (3) guarantee that liver moss water content is more than 50%; (4) fresh and alive liver moss matrix at 30 days with interior use.
(3) prepare in seedbed: cuttage the last week, above-mentioned bog moss matrix is laid into seedbed, can places one deck Polypropylence Sheet, nonwoven below slotting machine or be other planting soils and bare area.
(4) cuttage: by the fresh and alive bog moss matrix of the cuttings inserting step (2) of processing in step (1), water content of substrate remains on 70%, porosity 70%, insertion depth 3cm, is uncovered and is advisable with bud and blade, carries out plastic mulching and shades.
(5) after cuttage, manage: every day spray cooling, temperature is controlled 28 ℃, humidity is controlled at 90% left and right.After cuttage rooting, remove at noon plastic film at 12 o'clock to 15 o'clock, reduce humidity, increase illumination, and carry out the work of weeding, insect protected; Apply N: P: K is that 13: 26: 13 or N: P: K is the complete value fertilizer of 15: 30: 4, and fertilizer applies with liquid state, at interval of 20d, sprays 1 time.
the impact of table 1. different substrates water content on genus vaccinium plant cuttage survival rate.
the impact of the different cuttage degree of depth of table 2. on genus vaccinium plant cuttage survival rate.
Claims (2)
1. utilize fresh and alive bog moss to carry out a vegetative method to genus vaccinium plant, it is characterized in that:
(1) selection of cuttings and processing: choosing then raw semi-lignified, fringe bar sturdy perfectly straight, leaf bud is full, blade is done cuttings without the branch of damage by disease and insect, described fringe bar degree of lignification is low, be beneficial to the formation of callus, and then formation young root, long 6 ~ the 8cm of cuttings, soaks cuttings base portion 2h with the quickly dipped cutting of indolebutyric acid or with methyl α-naphthyl acetate;
(2) preparation of matrix: first the VA Mycorrhizal Fungi spore extracting is mixed with to mycorhiza suspension and is inoculated in the fresh and alive bog moss plant of cultivating in Experimental Establishment on a small quantity in great bilberry, make endotrophic mycorrhiza a large amount of propagation therein; Then, utilize the above-mentioned liver moss having inoculated to be inoculated in fresh and alive bog moss culture matrix as the maternal plant of endotrophic mycorrhiza;
(3) prepare in seedbed: cuttage the last week, above-mentioned bog moss matrix is laid into seedbed, can places one deck Polypropylence Sheet, nonwoven below slotting machine or be other planting soils and bare area;
(4) cuttage: by the fresh and alive bog moss matrix of the cuttings inserting step (2) of processing in step (1), water content of substrate remains on 50 ~ 70%, porosity 60 ~ 70%, insertion depth 1 ~ 3cm, with bud and blade, be uncovered and be advisable, carry out plastic mulching and shade;
(5) after cuttage, manage: every day spray cooling, temperature is controlled 15 ~ 28 ℃, humidity is controlled at 80 ~ 90% left and right.After cuttage rooting, remove at noon plastic film at 12 o'clock to 15 o'clock, reduce humidity, increase illumination, and carry out the work of weeding, insect protected; Apply N: P: K is that 13: 26: 13 or N: P: K is the complete value fertilizer of 15: 30: 4, and fertilizer applies with liquid state, at interval of 20d, sprays 1 time.
2. according to claim 1ly a kind ofly utilize fresh and alive bog moss to carry out vegetative method to genus vaccinium plant, it is characterized in that:
Described fresh and alive bog moss matrix, must meet following condition: (1) guarantees that the liver moss of field acquisition is green fresh and alive state; (2) in transportation, utilize the woven bag of good air permeability; (3) guarantee that liver moss water content is more than 50%; (4) fresh and alive liver moss matrix at 30 days with interior use.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104541887A (en) * | 2015-01-09 | 2015-04-29 | 云南云秀花卉有限公司 | Film-covered Chinese rose single bud cutting propagation method |
| CN104542210A (en) * | 2014-11-28 | 2015-04-29 | 大连大学 | Rapid propagation method for cranberry plant |
| CN104871762A (en) * | 2015-04-29 | 2015-09-02 | 中南林业科技大学 | Cutting propagation method for rhododendron auriculatum |
| CN106171473A (en) * | 2016-08-04 | 2016-12-07 | 余庆县农家人绿色食品开发有限公司 | The implantation methods of Fructus Capsici |
| CN106358890A (en) * | 2015-07-23 | 2017-02-01 | 程向东 | Vaccinium uliginosum cultivation technique |
| CN109661960A (en) * | 2018-08-24 | 2019-04-23 | 伊春林业科学院 | A kind of wild bog bilberry spray micro cuttage seedling-raising technique |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104871762A (en) * | 2015-04-29 | 2015-09-02 | 中南林业科技大学 | Cutting propagation method for rhododendron auriculatum |
| CN106358890A (en) * | 2015-07-23 | 2017-02-01 | 程向东 | Vaccinium uliginosum cultivation technique |
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| CN109661960A (en) * | 2018-08-24 | 2019-04-23 | 伊春林业科学院 | A kind of wild bog bilberry spray micro cuttage seedling-raising technique |
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| Publication number | Publication date |
|---|---|
| CN103636373B (en) | 2016-03-02 |
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