CN102668986B - Direct rooting method for tissue culture cluster seedlings of hemerocallis fulva - Google Patents

Direct rooting method for tissue culture cluster seedlings of hemerocallis fulva Download PDF

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Publication number
CN102668986B
CN102668986B CN2012101719713A CN201210171971A CN102668986B CN 102668986 B CN102668986 B CN 102668986B CN 2012101719713 A CN2012101719713 A CN 2012101719713A CN 201210171971 A CN201210171971 A CN 201210171971A CN 102668986 B CN102668986 B CN 102668986B
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seedling
root
medium
growing thickly
callus
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CN102668986A (en
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陈超
王桂兰
张红心
马云霄
孙献明
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Tangshan Normal University
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Tangshan Normal University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/22Improving land use; Improving water use or availability; Controlling erosion
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/40Afforestation or reforestation

Abstract

The invention discloses a direct rooting method for tissue culture cluster seedlings of hemerocallis fulva. The method comprises the steps of: inducing a hemerocallis fulva explant on a culture medium to produce callus, culturing cluster buds on a differential medium, slicing the callus, grafting the sliced callus on the culture medium for strong seedling culture; transitionally cultivating the cluster seedlings; in the transitionally cultivating process, planting the rooted cluster seedlings in a cultivation tank taking vermiculite as matrix, covering the cultivation tank with a mulching film; within the following 10 days, gradually reducing the humidity in the humidity tank to the normal humidity of a greenhouse by drilling the mulching film, uncovering the mulching film, and spraying a nutrient solution on leaf surfaces at the same time. The direct rooting method for tissue culture cluster seedlings of hemerocallis fulva disclosed in the invention, used for culturing cluster, is not only high in propagation multiples, but also not limited by seasons; so the method can accelerate the propagation and satisfy the requirement of garden afforestation; and meanwhile, the method provided by the invention simplifies the operation process of tissue culture, reduces the cost and increases the transplantation efficiency and survival rate of the tissue culture seedlings.

Description

The hemerocailis middendorffi group is trained the method that the seedling of growing thickly is directly taken root
Technical field
The present invention relates to a kind of ornamental plants tissue and cultivate the rooting method of the seedling of growing thickly, the hemerocailis middendorffi group training method that seedling is directly taken root of growing thickly particularly, be suitable for each kind of Perennial Flowers hemerocailis middendorffi.
Background technology
Hemerocailis middendorffi (Hemerocallis hybrida hort.) is Liliaceae hemerocallis perennial herb Perennial Flowers, has the characteristics such as stronger drought resisting, cold-resistant, anti-saline and alkaline, disease and insect resistance.But both establishing in large scale had been at lawn Zhou Ji, but also mass-planting, in lawn central authorities, increases stereoeffect, is the rare Perennial Flowers new varieties of afforestation.Through cultivation, observe, some large flower variety ripening rates are low, can only adopt division propagation, and year rate of increase only 2-3 doubly, reproduction rate is low, wilderness demand that far away can not satisfying the market.
Summary of the invention
The present invention be directed to the defect of current hemerocailis middendorffi division propagation, provide the hemerocailis middendorffi group that a kind of reproduction rate is high, the breeding cycle is short, survival rate is high to train the method that the seedling of growing thickly is directly taken root.
Realize that the technical scheme that the foregoing invention purpose adopts is: a kind of hemerocailis middendorffi group is trained the method that the seedling of growing thickly is directly taken root, and carries out in accordance with the following steps:
At first, the hemerocailis middendorffi group training seedling of growing thickly is directly taken root:
1. by the hemerocailis middendorffi explant at medium MS+6-BA4.0mgL -1+ NAA0.4 mgL -1on induce the generation callus, this callus is after propagation is cultivated, at differential medium MS+6-BA 2.0mgL -1+ NAA 0.2mgL -1upper cultivation produced Multiple Buds after 20 days, and its culture environment is: temperature 23-25 ℃, light intensity 2500lux, photoperiod 12h/d;
2. will be with the callus stripping and slicing of Multiple Buds, and stripping and slicing is transferred to not containing strong seedling culture on the MS medium of plant growth regulation, to cultivate and form afterwards the neat healthy and strong seedling of growing thickly in 20 days, the seedling of growing thickly in this incubation starts to take root;
Secondly, transplanting and management after the seedling rooting of growing thickly:
As long as 1. in every Cong Miao, have a strain to take root and root while reaching 1cm, this Cong Miao carried out to transition cultivating; During transition cultivating, the related medium of the seedling of growing thickly that will take root takes out from blake bottle, washes away the medium on root, is written into and take in the cultivating groove that vermiculite is matrix, uses the plastic film mulching cultivation groove, and in groove, humidity remains on more than 90%;
2. in afterwards 10 days, the method by film perforating progressively reduces humidity in cultivating groove and, to the greenhouse normal humidity, mulch film is thrown off, start to spray 1/2MS macro-element nutrients liquid simultaneously on blade face, once in a week, its substrate temperature is 10-25 ℃, and after 30 days, seedling enters Routine Management.
With existing division propagation, compare, method that seedling directly takes root is cultivated the seedling of growing thickly to utilize this hemerocailis middendorffi group training disclosed in this invention to grow thickly, and not only breeding rate is high, also is not subject to seasonal restrictions, and accelerates its reproduction speed, can meet the demand of afforestation; The present invention has simplified group training operation sequence, has reduced cost, has improved transplanting efficiency and the survival rate of group training seedling.
Embodiment
Below in conjunction with embodiment, the present invention will be further described.
This hemerocailis middendorffi group is trained the method that the seedling of growing thickly is directly taken root, and carries out in accordance with the following steps.
At first, the hemerocailis middendorffi group training seedling of growing thickly is directly taken root:
1. by the hemerocailis middendorffi explant at medium MS+6-BA4.0mgL -1+ NAA0.4 mgL -1on induce the generation callus, this callus is after propagation is cultivated, at differential medium MS+6-BA 2.0mgL -1+ NAA 0.2mgL -1upper cultivation produced Multiple Buds after 20 days, and its culture environment is: temperature 23-25 ℃, and light intensity 2500lux, photoperiod 12h/d, every day, light application time was 12 hours;
2. will be with the callus stripping and slicing of Multiple Buds, the size of block is 1cm 3left and right, and stripping and slicing is transferred to not containing strong seedling culture on the MS medium of plant growth regulation, to cultivate and form afterwards the neat healthy and strong seedling of growing thickly in 20 days, the seedling of growing thickly in this incubation starts to take root;
The seedling of growing thickly that the seedling of growing thickly that this method is cultivated has one or more root can reach more than 98%, and the seedling of growing thickly of taking root not is that each individual plant has root, between each individual plant of the seedling of growing thickly, can not separate.
Secondly, transplanting and management after the seedling rooting of growing thickly:
As long as 1. in every Cong Miao, have a strain to take root and root while reaching 1cm, this Cong Miao carried out to transition cultivating; During transition cultivating, the related medium of the seedling of growing thickly that will take root takes out from blake bottle, washes away the medium on root, is written into and take in the cultivating groove that vermiculite is matrix, uses the plastic film mulching cultivation groove, and in groove, humidity remains on more than 90%;
2. in afterwards 10 days, the method by film perforating progressively reduces humidity in cultivating groove and, to the greenhouse normal humidity, mulch film is thrown off, start to spray 1/2MS macro-element nutrients liquid simultaneously on blade face, once in a week, its substrate temperature is 10-25 ℃, and after 30 days, seedling enters Routine Management.1/2MS macro-element nutrients liquid is to utilize tissue to cultivate upper MS culture medium prescription commonly used, that is: the macroelement of MS minimal medium, trace element and molysite composition are as spray nutritious liquor, wherein only have the macroelement consumption to reduce by half, the consumption of trace element and molysite is constant;
Through the seedling of growing thickly of vermiculite transition, no matter each individual plant has before transition and does not have root all to bear new root, and is easy to separately, and every clump can produce independently seedling of 3-8 strain.
Embodiment: hemerocailis middendorffi kind " good luck " group is trained the direct rooting and transplant of the seedling of growing thickly.
The young tender anthocaulus of hemerocailis middendorffi " good luck " is that explant induction produces callus, through differential medium, produces Multiple Buds.To not be transferred to the callus stripping and slicing of Multiple Buds and form the neat and healthy and strong seedling of growing thickly containing strong seedling culture on the MS medium of plant growth regulator, the seedling of growing thickly in this process starts to take root.
Grow thickly after seedling rooting, can transplant when root grows to 1cm.During the 2-4 month in this year, transplant altogether 4 times, transition cultivating 4740 offspring of growing thickly, survival rate reaches more than 98%.Each individual plant of seedling of growing thickly after 20-30 days through the vermiculite transition all bears new root, and is easy to separately, and every clump can produce independently seedling of 3-8 strain.

Claims (1)

1. a hemerocailis middendorffi group is trained the method that the seedling of growing thickly is directly taken root, and it is characterized in that, carries out in accordance with the following steps:
At first, the hemerocailis middendorffi group training seedling of growing thickly is directly taken root:
1. by the hemerocailis middendorffi explant at medium MS+6-BA4.0mgL -1+ NAA0.4 mgL -1on induce the generation callus, this callus is after propagation is cultivated, at differential medium MS+6-BA2.0 mgL -1+ NAA0.2 mgL -1upper cultivation produced Multiple Buds after 20 days, and its culture environment is: temperature 23-25 ℃, light intensity 2500lux, photoperiod 12h/d;
2. will be with the callus stripping and slicing of Multiple Buds, and stripping and slicing is transferred to not containing strong seedling culture on the MS medium of plant growth regulation, to cultivate and form afterwards the neat healthy and strong seedling of growing thickly in 20 days, the seedling of growing thickly in this incubation starts to take root;
Secondly, transplanting and management after the seedling rooting of growing thickly:
As long as 1. in every Cong Miao, have a strain to take root and root while reaching 1cm, this Cong Miao carried out to transition cultivating; During transition cultivating, the related medium of the seedling of growing thickly that will take root takes out from blake bottle, washes away the medium on root, plants and take in the cultivating groove that vermiculite is matrix, uses the plastic film mulching cultivation groove, and in groove, humidity remains on more than 90%;
2. in afterwards 10 days, the method by film perforating progressively reduces humidity in cultivating groove and, to the greenhouse normal humidity, mulch film is thrown off, start to spray 1/2MS macro-element nutrients liquid simultaneously on blade face, once in a week, its substrate temperature is 10-25 ℃, and after 30 days, seedling enters Routine Management.
CN2012101719713A 2012-05-30 2012-05-30 Direct rooting method for tissue culture cluster seedlings of hemerocallis fulva Expired - Fee Related CN102668986B (en)

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CN103270882B (en) * 2013-06-07 2016-06-29 河北省林业科学研究院 A kind of full-film covering method for culturing seedlings of hemerocailis middendorffi Time To Market in advance
CN103461135B (en) * 2013-09-18 2015-10-07 宁波市农业科学研究院 A kind of propagation method of hemerocailis middendorffi
CN103609444B (en) * 2013-11-17 2015-04-29 浙江大学 Tissue culture method for hemerocallis sempervirens araki
CN104542285B (en) * 2014-12-30 2018-04-24 北京林业大学 A kind of method of hemerocailis middendorffi leaf tissue culture
CN105340754A (en) * 2015-12-04 2016-02-24 定州市绿谷农业科技发展有限公司 Method suitable for industrialized propagation of hemerocallis middendorfii
CN106937595A (en) * 2017-03-22 2017-07-11 江苏省农业科学院宿迁农科所 A kind of purification and rejuvenation method of great Wu mouths day lily

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