CN1729746A - Cotton grafting and fit grafting method - Google Patents
Cotton grafting and fit grafting method Download PDFInfo
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- CN1729746A CN1729746A CN 200510090827 CN200510090827A CN1729746A CN 1729746 A CN1729746 A CN 1729746A CN 200510090827 CN200510090827 CN 200510090827 CN 200510090827 A CN200510090827 A CN 200510090827A CN 1729746 A CN1729746 A CN 1729746A
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Abstract
The invention relates to a method for cotton grafting and connecting, comprising: shearing the stock to be smooth inclined surface and cutting it along the middle axle of the stem to the incline surface with the cut mouth being 'V' shape; shearing the scion to be inclined surface and cutting it along the middle axle with the cut mouth bring the shape of a reversal 'V'; contacting the two 'V', the multiple tangent plane overlapping with each and the stock and scion contacting naturally and tightly; insulating thermal and humidity during the contacting and sprout culture period, the survival rate of grafting is stable and above 95%, and if the fitting-in period of the stock and scion is proper, the rate can reach to 100%. The method is characterized by the simple processing, rapid grafting speed and high survival rate, and can be used for the grafting of test tube sprout such as transgenic tissue culture and young embryo, etc, and the grafting of normal cotton stem tip, fruit branch and leaf branch, as well as the grafting of soybean and other crops.
Description
Affiliated technical field
The present invention relates to a grafting method-cotton grafting and fit grafting method that grows cotton.
Background technology
Along with cotton cells engineering and engineered development, dissimilar test-tube plantlets such as the somatic cell regeneration plant of acquisition, transfer-gen plant and rataria cultivation plant get more and more, but its transplant survival is very difficult.Usually adopt methods such as direct field planting, vermiculite (sand) or nutrient solution transition cultivation to transplant, the cotton plant transplanting seedling time is long, and survival rate is very low, is restricting the application of biotechnology on the cotton genetic improvement to a certain extent.Therefore, the test-tube plantlet planting survival rates of raising transgenic cotton plant and other type becomes problem demanding prompt solution.In recent years, some reports adopt grafting method to solve test-tube plantlet field planting problem and have obtained certain effect." wedge joint method ", " tip cutting connection " or its improvement method etc. that the grafting method over-borrowing is used always with fruit tree and vegetable, its survival rate is 60%-80%.The present invention aims to provide a kind of grafting method that is suitable for easy, the high-servival rate of cotton, and this invention graft survival rate can be stabilized in more than 95%, cooperates the phase graft survival rate can reach 100% at suitable stock and scion.
Summary of the invention
The present invention is achieved through the following technical solutions: (1) is cultivated healthy and strong cotton seedling in advance and is done stock, the seedling age of the seedling age 〉=scion of stock; (2) with sharp thin blade stock growing point and unnecessary cauline leaf are excised, keep 〉=1 functional leaf; (3) the stem top that keeps is whittled into level and smooth inclined-plane from top to bottom, the long approximately 0.5cm-1.5cm in inclined-plane (inclined-plane can be long a little again when seedling age was big); (4) on the inclined-plane about 1/3 place along stem axis cutting perpendicularly to the inclined-plane bottom, otch is at the cross section middle part of stem, length 〉=0.3cm, otch are " V " font; (5) scion keeps 1-2 sheet leaf, and the root of bottom and unnecessary cauline leaf are removed, and scion is whittled into the inclined-plane suitable with stock from bottom to top, and vertically rives to the bottom, inclined-plane along axis, and long incision 〉=0.3cm, otch are down " V " font; (6) with scion and 2 interior tangent planes of stock, 2 staggered in twos, intimate engagement of outer tangent plane, be inserted to the tangent plane bottom, i.e. stock and scion " V " word is staggered to interlock.If the stem thickness of stock and scion is different, should make a side of joint stem level and smooth, the complete driving fit of phloem; (7) step up fixedly stock and scion joint with wide mouthful of suitable Grafting clip of degree of tightness, Grafting clip can not crush cortex and abundant fixed width is arranged; (8) water after the grafting, plastic film is obturaged on the cover, keeps humidity 〉=80%.Shading in the top, avoids the sunlight direct projection, 25 ℃-35 ℃ of temperature; (9) after the grafting 1-5 days, it is open and flat when tiding over seedling-slowing stage that scion leaf recovers, and removes the thing that shades, and leak informaton gradually and wet, and removes plastic film at last, finishes grafting.
This invention grafting cotton needs that cutter is accurate down, and otch is agilely level and smooth, keeps humidity 〉=80% after the grafting, graft survival rate can 〉=95%.When scion is the cotton seedling that cotyledon is deployed into 1 true leaf size, when stock was the cotton seedling of 1-3 sheet true leaf, graft survival rate can reach 100%, and this phase is the best seedling age phase that stock and scion cooperate.In addition, the stock seedling age is greater than the scion seedling age and to differ 1-2 sheet true leaf be good.
This invention not only can be applicable to the grafting of multiple test-tube plantlet such as the training of transgenosis group, rataria cultivation and conventional cotton plant stem apex, fruit branch, leaf branch, also can be used for the grafting of other crops such as soybean.
Embodiment
Can further know the present invention by specific embodiment given below, but they not limitations of the present invention.
Embodiment 1: cotton 20 cotton seedling 6 basins in potted plant cotton culture kind Ji, and every basin 5 strains, totally 30 strains are as stock.The cotton 20 other seeds in sterilization Ji, two week backs, the cotton seedling of aseptic culture is used as scion on medium, and when treating that the scion cotyledon launches, the greenhouse stock is grown to the cotton seedling of 2 true leaves.The stock true leaf is cut with top, keep the 2cm-3cm stem, stem is whittled into about 1.5cm inclined-plane, the suitable stem axis cutting perpendicularly in about 1/3 place is to bottom, stem cross section from the inclined-plane, and the long approximately 1.0cm of otch is " V " font.Scion goes root to keep the long stem of 2cm-3cm, is whittled into onesize inclined-plane, vertically rives along axis, and otch is about 1.0cm and is down " V " font.Carefully scion and 2 interior tangent planes of stock, 2 outer tangent planes are interlocked closely in twos and engage, be inserted to the tangent plane bottom, stock and scion " V " word is staggered to interlock.The flat mouthful Grafting clip wide with about 1.0cm steps up fixedly stock and scion joint.Water after the grafting, prop small wooden around the grafting plant, plastic film and sealing on the cover, shade with newspaper in the film top.Water every day once, keep relative moisture 85%-100%, grafting plant is tided over seedling-slowing stage after 2 days, and blade launches again.Remove newspaper, it is wet to leak informaton gradually, removes plastic film fully in 4 days after the grafting, and the grafting cotton plant all survives, survival rate 100%.
Claims (2)
1. a cotton grafting and fit grafting method is characterized in that, comprises following sequential steps:
(1) stock is whittled into smooth ramp and along stem axis cutting perpendicularly to the inclined-plane bottom, otch becomes " V " font, stock keeps 〉=1 functional leaf; (2) scion is whittled into and the sizable inclined-plane of stock, vertically rives to the bottom, inclined-plane along axis, and otch is down " V " font, keeps 〉=1 blade; (3) scion and 2 interior tangent planes of stock, 2 outer tangent planes are interlocked closely in twos and engage, be inserted to the tangent plane bottom, promptly two " V " word interlocks, if the stem thickness of stock and scion is different, should make a side of joint stem level and smooth, the complete driving fit of phloem; (4) Grafting clip steps up fixedly stock and scion joint; (5) keep humidity 〉=80% after the grafting, 25 ℃-35 ℃ of temperature, shade in the top; (6) the grafting cotton is tided over seedling-slowing stage, when scion leaf recovers open and flat, removes the thing that shades, and leaks informaton gradually and dries, and finishes grafting.
2. cotton grafting and fit grafting method according to claim 1 is characterized in that 2 interior tangent planes and 2 relative in twos interlocking of outer tangent plane of described scion and stock are inserted the tangent plane bottom, intimate engagement, i.e. and stock and scion " V " word is staggered to interlock insertion depth 〉=0.3cm.Grafting clip is clipped in bonding station.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510090827 CN1729746A (en) | 2005-08-18 | 2005-08-18 | Cotton grafting and fit grafting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510090827 CN1729746A (en) | 2005-08-18 | 2005-08-18 | Cotton grafting and fit grafting method |
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| CN1729746A true CN1729746A (en) | 2006-02-08 |
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| CN 200510090827 Pending CN1729746A (en) | 2005-08-18 | 2005-08-18 | Cotton grafting and fit grafting method |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101491197B (en) * | 2009-02-24 | 2010-09-15 | 广西大学 | Cotton beveling engrafting method |
| CN101855969A (en) * | 2010-06-03 | 2010-10-13 | 山东棉花研究中心 | Grafting root-planting method for cotton |
| CN101491192B (en) * | 2009-02-24 | 2011-01-19 | 广西大学 | Cotton grafting soil-borne disease prevention and treatment method |
| CN101878708B (en) * | 2009-12-24 | 2012-05-23 | 江苏沿海地区农业科学研究所 | Asexual grafting method of island cotton and upland cotton |
| CN102613016A (en) * | 2012-03-29 | 2012-08-01 | 中国农业科学院作物科学研究所 | Method for identifying plant root traits and evaluating contribution ability of root system to yield |
| CN102893813A (en) * | 2012-10-25 | 2013-01-30 | 山西省农业科学院棉花研究所 | Grafting technique for excrescent buds of regenerated plants of genetically modified cotton |
| CN103688765A (en) * | 2013-12-11 | 2014-04-02 | 新疆农业科学院经济作物研究所 | Method for improving survival rate of cotton after grafting |
| CN104303851A (en) * | 2014-09-28 | 2015-01-28 | 定远县金胜农业开发有限公司 | Tomato grafting method capable of improving survival rate |
| CN104488575A (en) * | 2014-12-22 | 2015-04-08 | 广西壮族自治区林业科学研究院 | Eucalyptus cloeziana grafting method |
| CN105265192A (en) * | 2015-11-19 | 2016-01-27 | 北京农学院 | High-survival-rate cotton grafting method |
| CN115380721A (en) * | 2022-10-09 | 2022-11-25 | 湖北省农业科学院经济作物研究所 | Efficient cotton transgenic plant grafting technology |
-
2005
- 2005-08-18 CN CN 200510090827 patent/CN1729746A/en active Pending
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101491192B (en) * | 2009-02-24 | 2011-01-19 | 广西大学 | Cotton grafting soil-borne disease prevention and treatment method |
| CN101491197B (en) * | 2009-02-24 | 2010-09-15 | 广西大学 | Cotton beveling engrafting method |
| CN101878708B (en) * | 2009-12-24 | 2012-05-23 | 江苏沿海地区农业科学研究所 | Asexual grafting method of island cotton and upland cotton |
| CN101855969A (en) * | 2010-06-03 | 2010-10-13 | 山东棉花研究中心 | Grafting root-planting method for cotton |
| CN101855969B (en) * | 2010-06-03 | 2011-07-20 | 山东棉花研究中心 | Grafting root-planting method for cotton |
| CN102613016B (en) * | 2012-03-29 | 2016-08-03 | 中国农业科学院作物科学研究所 | A kind of plant identification root traits and the assessment root system method to contribution to yield ability |
| CN102613016A (en) * | 2012-03-29 | 2012-08-01 | 中国农业科学院作物科学研究所 | Method for identifying plant root traits and evaluating contribution ability of root system to yield |
| CN102893813A (en) * | 2012-10-25 | 2013-01-30 | 山西省农业科学院棉花研究所 | Grafting technique for excrescent buds of regenerated plants of genetically modified cotton |
| CN102893813B (en) * | 2012-10-25 | 2013-10-23 | 山西省农业科学院棉花研究所 | Grafting technique for excrescent buds of regenerated plants of genetically modified cotton |
| CN103688765A (en) * | 2013-12-11 | 2014-04-02 | 新疆农业科学院经济作物研究所 | Method for improving survival rate of cotton after grafting |
| CN104303851A (en) * | 2014-09-28 | 2015-01-28 | 定远县金胜农业开发有限公司 | Tomato grafting method capable of improving survival rate |
| CN104303851B (en) * | 2014-09-28 | 2017-01-25 | 定远县金胜农业开发有限公司 | Tomato grafting method capable of improving survival rate |
| CN104488575A (en) * | 2014-12-22 | 2015-04-08 | 广西壮族自治区林业科学研究院 | Eucalyptus cloeziana grafting method |
| CN105265192A (en) * | 2015-11-19 | 2016-01-27 | 北京农学院 | High-survival-rate cotton grafting method |
| CN115380721A (en) * | 2022-10-09 | 2022-11-25 | 湖北省农业科学院经济作物研究所 | Efficient cotton transgenic plant grafting technology |
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