CN102893813A - Grafting technique for excrescent buds of regenerated plants of genetically modified cotton - Google Patents
Grafting technique for excrescent buds of regenerated plants of genetically modified cotton Download PDFInfo
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- CN102893813A CN102893813A CN2012104100828A CN201210410082A CN102893813A CN 102893813 A CN102893813 A CN 102893813A CN 2012104100828 A CN2012104100828 A CN 2012104100828A CN 201210410082 A CN201210410082 A CN 201210410082A CN 102893813 A CN102893813 A CN 102893813A
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Abstract
The invention relates to a grafting method for plants, and particularly relates to a grafting technique for excrescent buds of regenerated plants of genetically modified cotton. According to the grafting technique, cotton seedlings of the genetically modified cotton are taken as scion plants, and cotton seedlings of non-genetically-modified cotton are taken as stock plants. The grafting method comprises the steps of preparation of the cotton seedlings, grafting, and cultivation after grafting, wherein in the preparation of the cotton seedlings in the first step, after two to three main leaves grow on the stock plant, the parts above seed leaves of the main leaves are removed, and the excrescent buds are grown between the left seed leaves and a stem; after four to six leaves grow on the excrescent buds, the top part of one excrescent bud is removed, and the top end of the left stem part of the excrescent bud is cut to be V-shaped incision; and the root part of the scion plant is cut off, and the bottom of a hypocotyledonary axis of the scion plant is cut to be inverted-V-shaped incision matched with the incision of the stock plant.
Description
Technical field
The present invention relates to the grafting method of a Plants, specifically the superfluous bud graft technology of a kind of transgenic cotton regenerated plant.
Background technology
At present, what the transgenic cotton regenerated plant grafting method adopted is traditional cuttage, selects the cotton seedling of transgene cotton as the scion strain, and the cotton seedling of non-transgenic cotton is as the stock strain; Comprise the steps: the preparation of (1) cotton seedling: cut scion strain root, the hypocotyl bottom is whittled into otch for falling " V " font; Not lignified stock strain cuts the stem top, and remaining stem top is whittled into otch and is " V " font; (2) grafting: the close combination of otch of scion strain and stock strain, with plastics cord or other cord wrapping secure bond place, adopt container or plastic film (material which can retain moisture) parcel grafting, finish grafting; (3) cultivation after the grafting: grafting is moved into the shady and cool place in solarium or greenhouse, after 7 days, progressively leak informaton and dry, remove container or plastic film.Grafting survives rear removal cord and gets final product.Grafting move on to see the light place progressively incubation growth, Cheng Lei, bloom, tie bell, blow-of-cottons.
The advantage of this grafting method: because the stock strain is not lignified stock strain, scion strain and stock strain bondability are good, and the fall and winter survival rate is higher.But it is shorter for a long time that not lignified stock strain becomes, in the summer high temperature humid season, stock strain hypocotyl part is tenderer, with material which can retain moisture grafting is wrapped up several days, and the phloem of stock strain hypocotyl and soil contact portion is more perishable, grafting is dead, causes the survival rate of grafting low.
Summary of the invention
Even the present invention aims to provide a kind of at the superfluous bud graft technology of the high transgenic cotton regenerated plant of summer high temperature humid season grafting survival rate.
The present invention is achieved by the following technical solutions: the transgenic cotton regenerated plant bud graft technology of going to live in the household of one's in-laws on getting married, and select the cotton seedling of transgene cotton as the scion strain, the cotton seedling of non-transgenic cotton is as the stock strain; Described grafting method comprises the steps: the preparation of (1) cotton seedling; (2) grafting; (3) cultivation after the grafting; Being prepared as of described step (1) cotton seedling: after the stock strain grows 2～3 true leaves, its cotyledon is removed with upper part (comprising blade and stem), treat to grow superfluous bud between the cotyledon of remainder and the stem, and after growing 4～6 blades on the superfluous bud, remove the top of one of them superfluous bud, the stem top of this superfluous bud remainder is whittled into the otch that is " V " font; Cut scion strain root, the hypocotyl bottom of scion strain is whittled into the otch that is down " V " font that matches with stock strain otch.
When growing 4～6 blades on the superfluous bud, stock the hypocotyl strong and lignification of having grown is not even how under the wet environment, can cause rotting of hypocotyl phloem at summer high temperature after the grafting scion strain in the stock strain.
The otch of " V " font of stock strain and scion strain fall " V " font otch specifically cut the common practise that step is this area, do not repeat them here.
The superfluous bud graft technology of transgenic cotton regenerated plant of the present invention is compared with conventional method, has the following advantages:
(1) simple to operate, even in the season that summer, such high temperature wet more, survival rate all more than (95%), increases significantly than traditional survival rate (85%);
(2) the method can be used for the grafting of any cotton seedling or plant, also can be used for the grafting of other plant.
Embodiment
Embodiment 1
The season that summer high temperature wets more, cotton seedling 30 basins of potted plant non-transgenic cotton (disease-resistant long-staple cotton kind), every basin 1 strain, totally 30 strains are as the stock strain.The cotton seedling of aseptic culture transgene cotton is as the scion strain on the medium.
(1) preparation of cotton seedling: after the stock strain grows 2 true leaves, its cotyledon is removed with the upper part, treat to grow superfluous bud between the cotyledon of remainder and the stem, and after growing 6 blades on the superfluous bud, remove the top of one of them superfluous bud, the stem top of this superfluous bud remainder is whittled into the otch that is " V " font; Cut scion strain root, the hypocotyl bottom of scion strain is whittled into the otch that is down " V " font that matches with stock strain otch.
(2) grafting: the close combination of otch of scion strain and stock strain, with plastics cord wrapping secure bond place, adopt the container pack grafting, finish grafting.
(3) cultivation after the grafting: grafting is moved into shady and cool place, solarium, after 7 days, progressively leak informaton and dry, remove container.The transgenic cotton regenerated plant survival rate 100% that grafting is finished.
During implementation, finish grafting and afterwards other superfluous bud (except grafting has transgene cotton) is removed.
Embodiment 2
The season that summer high temperature wets more, cotton seedling 30 basins of potted plant non-transgenic cotton (disease-resistant long-staple cotton kind), every basin 1 strain, totally 30 strains are as the stock strain.The cotton seedling of aseptic culture transgene cotton is as the scion strain on the medium.
(1) preparation of cotton seedling: after the stock strain grows 3 true leaves, its cotyledon is removed with the upper part, treat to grow superfluous bud between the cotyledon of remainder and the stem, and after growing 4 blades on the superfluous bud, remove the top of one of them superfluous bud, the stem top of this superfluous bud remainder is whittled into the otch that is " V " font; Cut scion strain root, the hypocotyl bottom of scion strain is whittled into the otch that is down " V " font that matches with stock strain otch.
(2) grafting: the close combination of otch of scion strain and stock strain, with nylon marline wrapping secure bond place, adopt plastic film parcel grafting, finish grafting.
(3) cultivation after the grafting: grafting is moved into shady and cool place, greenhouse, after 7 days, progressively leak informaton and dry, remove plastic film.The transgenic cotton regenerated plant survival rate 97% that grafting is finished.
Embodiment 3
The season that summer high temperature wets more, cotton seedling 30 basins of potted plant non-transgenic cotton (disease-resistant long-staple cotton kind), every basin 1 strain, totally 30 strains are as the stock strain.The cotton seedling of aseptic culture transgene cotton is as the scion strain on the medium.
(1) preparation of cotton seedling: after the stock strain grows 3 true leaves, its cotyledon is removed with the upper part, treat to grow superfluous bud between the cotyledon of remainder and the stem, and after growing 5 blades on the superfluous bud, remove the top of one of them superfluous bud, the stem top of this superfluous bud remainder is whittled into the otch that is " V " font; Cut scion strain root, the hypocotyl bottom of scion strain is whittled into the otch that is down " V " font that matches with stock strain otch.
(2) grafting: the close combination of otch of scion strain and stock strain, with plastics cord wrapping secure bond place, adopt the container pack grafting, finish grafting.
(3) cultivation after the grafting: grafting is moved into shady and cool place, solarium, after 7 days, progressively leak informaton and dry, remove container.The transgenic cotton regenerated plant survival rate 97% that grafting is finished.
Claims (1)
1. the superfluous bud graft technology of transgenic cotton regenerated plant selects the cotton seedling of transgene cotton as the scion strain, and the cotton seedling of non-transgenic cotton is as the stock strain; Described grafting method comprises the steps: the preparation of (1) cotton seedling; (2) grafting; (3) cultivation after the grafting; It is characterized in that, being prepared as of described step (1) cotton seedling: after the stock strain grows 2～3 true leaves, its cotyledon is removed with the upper part, treat to grow superfluous bud between the cotyledon of remainder and the stem, and after growing 4～6 blades on the superfluous bud, remove the top of one of them superfluous bud, the stem top of this superfluous bud remainder is whittled into the otch that is " V " font; Cut scion strain root, the hypocotyl bottom of scion strain is whittled into the otch that is down " V " font that matches with stock strain otch.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210410082 CN102893813B (en) | 2012-10-25 | 2012-10-25 | Grafting technique for excrescent buds of regenerated plants of genetically modified cotton |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210410082 CN102893813B (en) | 2012-10-25 | 2012-10-25 | Grafting technique for excrescent buds of regenerated plants of genetically modified cotton |
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| Publication Number | Publication Date |
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| CN102893813A true CN102893813A (en) | 2013-01-30 |
| CN102893813B CN102893813B (en) | 2013-10-23 |
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| CN 201210410082 Expired - Fee Related CN102893813B (en) | 2012-10-25 | 2012-10-25 | Grafting technique for excrescent buds of regenerated plants of genetically modified cotton |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105103972A (en) * | 2015-09-25 | 2015-12-02 | 山西省农业科学院棉花研究所 | Temperature-controlled grafting method for regenerated plants of transgenic cotton |
| CN107509535A (en) * | 2017-09-01 | 2017-12-26 | 山西省农业科学院棉花研究所 | Dicotyledon seedling and the efficient cleft grafting method of edible tender branch |
| CN115380721A (en) * | 2022-10-09 | 2022-11-25 | 湖北省农业科学院经济作物研究所 | Efficient cotton transgenic plant grafting technology |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1729746A (en) * | 2005-08-18 | 2006-02-08 | 河北农业大学 | Cotton grafting and fit grafting method |
| CN101491197A (en) * | 2009-02-24 | 2009-07-29 | 广西大学 | Cotton beveling engrafting method |
| CN101491192A (en) * | 2009-02-24 | 2009-07-29 | 广西大学 | Cotton grafting soil-borne disease prevention and treatment method |
| CN101878708A (en) * | 2009-12-24 | 2010-11-10 | 江苏沿海地区农业科学研究所 | Asexual grafting method of island cotton and upland cotton |
-
2012
- 2012-10-25 CN CN 201210410082 patent/CN102893813B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1729746A (en) * | 2005-08-18 | 2006-02-08 | 河北农业大学 | Cotton grafting and fit grafting method |
| CN101491197A (en) * | 2009-02-24 | 2009-07-29 | 广西大学 | Cotton beveling engrafting method |
| CN101491192A (en) * | 2009-02-24 | 2009-07-29 | 广西大学 | Cotton grafting soil-borne disease prevention and treatment method |
| CN101878708A (en) * | 2009-12-24 | 2010-11-10 | 江苏沿海地区农业科学研究所 | Asexual grafting method of island cotton and upland cotton |
Non-Patent Citations (5)
| Title |
|---|
| JINHUA LUO ETAL.: "In vitro shoot-tip grafting improves recovery of cotton plants from culture", 《PLANT CELL，TISSUE AND ORGAN CULTURE》 * |
| 李燕娥等: "转基因再生棉花嫁接初报", 《中国棉花》 * |
| 王彦霞等: "棉花高效嫁接新方法及其应用", 《中国农业科学》 * |
| 纪龙邦等: "海岛棉与陆地棉嫁接在江苏射阳获得成功", 《中国棉花》 * |
| 郭长生: "棉花嫁接的优势及其嫁接关键技术", 《现代农业科技》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105103972A (en) * | 2015-09-25 | 2015-12-02 | 山西省农业科学院棉花研究所 | Temperature-controlled grafting method for regenerated plants of transgenic cotton |
| CN107509535A (en) * | 2017-09-01 | 2017-12-26 | 山西省农业科学院棉花研究所 | Dicotyledon seedling and the efficient cleft grafting method of edible tender branch |
| CN115380721A (en) * | 2022-10-09 | 2022-11-25 | 湖北省农业科学院经济作物研究所 | Efficient cotton transgenic plant grafting technology |
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| Publication number | Publication date |
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| CN102893813B (en) | 2013-10-23 |
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