CN104429486A - Method for culturing immature seeds of processed tomatoes into seedlings - Google Patents
Method for culturing immature seeds of processed tomatoes into seedlings Download PDFInfo
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- CN104429486A CN104429486A CN201410639363.XA CN201410639363A CN104429486A CN 104429486 A CN104429486 A CN 104429486A CN 201410639363 A CN201410639363 A CN 201410639363A CN 104429486 A CN104429486 A CN 104429486A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Soil Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to the technical field of immature seed cultivation methods, in particular to a method for culturing immature seeds of processed tomatoes into seedlings. The method comprises the steps that firstly, immature fruits of the processed tomatoes growing 20-28 days after pollination of flowers of the processed tomatoes are picked, disinfected and cleaned; secondly, the disinfected and cleaned immature fruits of the processed tomatoes are cut, and the immature seeds of the processed tomatoes are taken out. According to the method, the immature seeds are directly taken out of the immature fruits of the processed tomatoes growing 20-28 days after pollination, and then the immature seeds are cultivated to obtain the seedlings. By means of the method, the processed tomatoes can be planted for 3-5 generations in one year, and only one generation of processed tomatoes can be planted in one year through a conventional seedling-culture method, so it is proved that the method for culturing the immature seeds of the processed tomatoes into the seedlings greatly increases the seed breeding speed compared with the conventional seedling-culture method, a technical basis is laid for development of the tomato processing industry, and existing production demands can be met.
Description
Technical field
the present invention relates to immature seed breeding method technical field, is a kind of method that processing tomato immature seed cultivates seedling.
Background technology
xinjiang region sunshine is long, day and night temperature is large, dry, rainfall is few, air, soil and water quality environment pollute few, has the advantageous natural advantage of development processing tomato industry.Through the development of decades, Xinjiang has become one of three large processing tomato producing regions, the whole world, is also the large catsup outlet area that beats the world.Along with the development of processing tomato industry, the demand of processing tomato differentiation new varieties highlights day by day, especially lacks the excellent extremely early mature mechanical harvesting kind of kind, and processing tomato breeding technique backwardness relatively, breeding speed is comparatively slow, can not meet existing Production requirement.
Summary of the invention
the invention provides a kind of method that processing tomato immature seed cultivates seedling, overcome the deficiency of above-mentioned prior art, it effectively can solve existing processing tomato breeding technique backwardness relatively, and breeding speed is comparatively slow, can not meet the problem of existing Production requirement.
technical scheme of the present invention is realized by following measures: a kind of processing tomato immature seed cultivates the method for seedling, carry out as follows: the first step, win the prematurity processing tomato fruit of the rear 20d to 28d of processing tomato flower pollination, prematurity processing tomato fruit is sterilized and cleaned; Second step, prematurity processing tomato fruit after sterilization being cleaned cuts and takes out prematurity Processing tomato seed, prematurity Processing tomato seed to be inoculated in medium and airtight, temperature be 26 DEG C to 30 DEG C, light application time is 16h/d, intensity of illumination is cultivated 5d to 7d and carried out obtaining after induction is sprouted training seedling under being the condition of 3000lx; 3rd step, seedling 1d to 2d is practiced after the root of training seedling grows to 5cm to 7cm, after practicing seedling, training seedling is taken out the medium washing root with water, irrigate with water in the seedling medium again training seedling being gone in nutritive cube, at room temperature cultivate 12d to 15d, then training seedling is transplanted together with seedling medium and carry out normal management to greenhouse or land for growing field crops.
here is the further optimization and/or improvements to foregoing invention technical scheme:
above-mentioned prematurity processing tomato fruit is sterilized and is cleaned and carries out as follows: first rinsed well under flowing water by prematurity processing tomato fruit, 1min is soaked again with the ethanol water that percent by volume is 75%, then put into thimerosal to soak 15 minutes, use aseptic water washing 5 times to 6 times again, rinse 2min at every turn; Thimerosal by the percent by volume of 30ml to 50ml be add in the aqueous sodium hypochlorite solution of 20% 2 drop to the mixing of 3 tweens after obtain.
above-mentioned medium is the first medium or the second medium; First medium obtains the first medium after adding the mixing of 0.9g agar in 5.2gMS medium; Second medium presses 5.2g B
5
the second medium is obtained after adding 2.5g sucrose and the mixing of 1.75g agar in medium.
in the above-mentioned first step, pollination processing tomato flower is that the petal exhibition of processing tomato flower is pollination to 90 degree.
in above-mentioned second step, prematurity processing tomato fruit after sterilization being cleaned laterally or vertically cuts and takes out prematurity Processing tomato seed, peel off the pectin of prematurity Processing tomato seed, then the prematurity Processing tomato seed after peeling off pectin to be inoculated in the medium in blake bottle and to tighten bottle cap and carry out airtight.
in above-mentioned 3rd step, the root until training seedling grows to 5cm to 7cm, training is after seedling grows to 6cm to 8cm, adds the distilled water of 1cm to 1.5cm in blake bottle by the height of blake bottle, and bottle cap is unscrewed or opened osculum and adapts to 1d, then practices seedling 1d to 2d.
in above-mentioned 3rd step, at room temperature cultivate 12d to 15d, then remove nutritive cube and training seedling is carried out normal management together with seedling medium transplanting to greenhouse or land for growing field crops; The first day nutritive cube transparent plastic bag sealing of at room temperature cultivating is cultivated, and within second day, opens osculum gradually, removes transparent plastic bag after the 6th day.
the present invention directly takes out immature seed from the prematurity processing tomato fruit of the rear 20d to 28d of pollination, then carry out cultivation to immature seed to obtain training seedling, 1 year 3 generations in generation to 5 plantation processing tomato can be realized by the present invention, and conventional seedbed system method within 1 year, can only plant 1 generation processing tomato, illustrate processing tomato immature seed of the present invention cultivate seedling method comparatively conventional seedbed system method greatly accelerate breeding speed, technical foundation has been established in development for processing tomato industry, can meet existing Production requirement.
Embodiment
the present invention by the restriction of following embodiment, can not determine concrete embodiment according to technical scheme of the present invention and actual conditions.
embodiment 1, this processing tomato immature seed cultivates the method for seedling, carries out as follows: the first step, wins the prematurity processing tomato fruit of the rear 20d to 28d of processing tomato flower pollination, prematurity processing tomato fruit is sterilized and cleaned; Second step, prematurity processing tomato fruit after sterilization being cleaned cuts and takes out prematurity Processing tomato seed, prematurity Processing tomato seed to be inoculated in medium and airtight, temperature be 26 DEG C to 30 DEG C, light application time is 16h/d, intensity of illumination is cultivated 5d to 7d and carried out obtaining after induction is sprouted training seedling under being the condition of 3000lx; 3rd step, seedling 1d to 2d is practiced after the root of training seedling grows to 5cm to 7cm, after practicing seedling, training seedling is taken out the medium washing root with water, irrigate with water in the seedling medium again training seedling being gone in nutritive cube, at room temperature cultivate 12d to 15d, then training seedling is transplanted together with seedling medium and carry out normal management to greenhouse or land for growing field crops.
embodiment 2, as the optimization of above-described embodiment, in embodiment 2, prematurity processing tomato fruit is sterilized and is cleaned and carries out as follows: first rinsed well under flowing water by prematurity processing tomato fruit, 1min is soaked again with the ethanol water that percent by volume is 75%, then put into thimerosal to soak 15 minutes, use aseptic water washing 5 times to 6 times again, rinse 2min at every turn; Thimerosal by the percent by volume of 30ml to 50ml be add in the aqueous sodium hypochlorite solution of 20% 2 drop to the mixing of 3 tweens after obtain.
embodiment 3, as the optimization of above-described embodiment, in embodiment 3, medium is the first medium or the second medium; First medium obtains the first medium after adding the mixing of 0.9g agar in 5.2gMS medium; Second medium presses 5.2g B
5
the second medium is obtained after adding 2.5g sucrose and the mixing of 1.75g agar in medium.
embodiment 4, as the optimization of above-described embodiment, in the first step of embodiment 4, pollination processing tomato flower is that the petal exhibition of processing tomato flower is pollination to 90 degree.
embodiment 5, as the optimization of above-described embodiment, in the second step of embodiment 5, prematurity processing tomato fruit after sterilization being cleaned laterally or vertically cuts and takes out prematurity Processing tomato seed, peel off the pectin of prematurity Processing tomato seed, then the prematurity Processing tomato seed after peeling off pectin to be inoculated in the medium in blake bottle and to tighten bottle cap and carry out airtight.After pollination, the processing tomato immature seed of 25 d has pectin, pectin affects percentage of seedgermination, gets clean pectin during stripping as far as possible, and the time that immature seed stops in atmosphere longer germination rate is lower, so the speed of cutting The faster the better, shell more clean better.
embodiment 6, as the optimization of above-described embodiment, in the 3rd step of embodiment 6, root until training seedling grows to 5cm to 7cm, training is after seedling grows to 6cm to 8cm, in blake bottle, add the distilled water of 1cm to 1.5cm by the height of blake bottle, bottle cap is unscrewed or is opened osculum adaptation 1d, then practices seedling 1d to 2d.
embodiment 7, as the optimization of above-described embodiment, in the 3rd step of embodiment 7, at room temperature cultivates 12d to 15d, then removes nutritive cube and training seedling is carried out normal management together with seedling medium transplanting to greenhouse or land for growing field crops; The first day nutritive cube transparent plastic bag sealing of at room temperature cultivating is cultivated, and within second day, opens osculum gradually, removes transparent plastic bag after the 6th day.When inoculation more than 6, when every seed sprouting grows to 6 ~ 7cm, 1st true leaf has grown to about 3 cm, whole blake bottle is more crowded, be unfavorable for growth and transplant getting seedling work, when inoculating less than 4, treating capacity is many, large and the waste material of workload, thus every blake bottle inoculate 4 more applicable to 6.
the training seedling that the method for cultivating seedling by the above embodiment of the present invention processing tomato immature seed obtains is shown in Table 1, as can be seen from Table 1, be inoculated in by prematurity Processing tomato seed and carry out induction in the first medium or the second medium and sprout, germination rate all reaches 100%.The present invention directly takes out immature seed from the prematurity processing tomato fruit of the rear 20d to 28d of pollination, then carry out cultivation to immature seed to obtain training seedling, 1 year 3 generations in generation to 5 plantation processing tomato can be realized by the present invention, and conventional seedbed system method can only carry out training by just getting seed after processing tomato maturation the cultivation of seedling, so conventional seedbed system method within 1 year, can only plant 1 generation processing tomato, illustrate processing tomato immature seed of the present invention cultivate seedling method comparatively conventional seedbed system method greatly accelerate breeding speed, technical foundation has been established in development for processing tomato industry, existing Production requirement can be met.
above technical characteristic constitutes embodiments of the invention, and it has stronger adaptability and implementation result, can increase and decrease non-essential technical characteristic according to actual needs, meet the demand of different situations.
table 1
| Medium | Pollination number of days | Seed amount (grain) | Germination quantity | Germination rate |
| Medium | Days after pollinationg\d | The numble of seeds/ grain | Sprout number/ | Germination rate/% |
| First medium | 25 | 60 | 60 | 100 |
| Second medium | 25 | 60 | 60 | 100 |
Claims (10)
1. processing tomato immature seed cultivates a method for seedling, it is characterized in that carrying out as follows: the first step, wins the prematurity processing tomato fruit of the rear 20d to 28d of processing tomato flower pollination, prematurity processing tomato fruit is sterilized and cleaned; Second step, prematurity processing tomato fruit after sterilization being cleaned cuts and takes out prematurity Processing tomato seed, prematurity Processing tomato seed to be inoculated in medium and airtight, temperature be 26 DEG C to 30 DEG C, light application time is 16h/d, intensity of illumination is cultivated 5d to 7d and carried out obtaining after induction is sprouted training seedling under being the condition of 3000lx; 3rd step, seedling 1d to 2d is practiced after the root of training seedling grows to 5cm to 7cm, after practicing seedling, training seedling is taken out the medium washing root with water, irrigate with water in the seedling medium again training seedling being gone in nutritive cube, at room temperature cultivate 12d to 15d, then training seedling is transplanted together with seedling medium and carry out normal management to greenhouse or land for growing field crops.
2. processing tomato immature seed according to claim 1 cultivates the method for seedling, it is characterized in that prematurity processing tomato fruit is sterilized and cleaned to carry out as follows: prematurity processing tomato fruit is first rinsed well under flowing water, 1min is soaked again with the ethanol water that percent by volume is 75%, then put into thimerosal to soak 15 minutes, use aseptic water washing 5 times to 6 times again, rinse 2min at every turn; Thimerosal by the percent by volume of 30ml to 50ml be add in the aqueous sodium hypochlorite solution of 20% 2 drop to the mixing of 3 tweens after obtain.
3. processing tomato immature seed according to claim 1 and 2 cultivates the method for seedling, it is characterized in that medium is the first medium or the second medium; First medium obtains the first medium after adding the mixing of 0.9g agar in 5.2gMS medium; Second medium presses 5.2g B
5the second medium is obtained after adding 2.5g sucrose and the mixing of 1.75g agar in medium.
4. processing tomato immature seed according to claim 1 and 2 cultivates the method for seedling, it is characterized in that in the first step, and pollination processing tomato flower is that the petal exhibition of processing tomato flower is pollination to 90 degree.
5. processing tomato immature seed according to claim 3 cultivates the method for seedling, it is characterized in that in the first step, and pollination processing tomato flower is that the petal exhibition of processing tomato flower is pollination to 90 degree.
6. the processing tomato immature seed according to claim 1 or 2 or 5 cultivates the method for seedling, it is characterized in that in second step, prematurity processing tomato fruit after sterilization being cleaned laterally or vertically cuts and takes out prematurity Processing tomato seed, peel off the pectin of prematurity Processing tomato seed, then the prematurity Processing tomato seed after peeling off pectin to be inoculated in the medium in blake bottle and to tighten bottle cap and carry out airtight.
7. processing tomato immature seed according to claim 3 cultivates the method for seedling, it is characterized in that in second step, prematurity processing tomato fruit after sterilization being cleaned laterally or vertically cuts and takes out prematurity Processing tomato seed, peel off the pectin of prematurity Processing tomato seed, then the prematurity Processing tomato seed after peeling off pectin to be inoculated in the medium in blake bottle and to tighten bottle cap and carry out airtight.
8. processing tomato immature seed according to claim 4 cultivates the method for seedling, it is characterized in that in second step, prematurity processing tomato fruit after sterilization being cleaned laterally or vertically cuts and takes out prematurity Processing tomato seed, peel off the pectin of prematurity Processing tomato seed, then the prematurity Processing tomato seed after peeling off pectin to be inoculated in the medium in blake bottle and to tighten bottle cap and carry out airtight.
9. the processing tomato immature seed according to claim 6 or 7 or 8 cultivates the method for seedling, it is characterized in that in the 3rd step, root until training seedling grows to 5cm to 7cm, training is after seedling grows to 6cm to 8cm, add the distilled water of 1cm to 1.5cm by the height of blake bottle in blake bottle, bottle cap is unscrewed or is opened osculum adaptation 1d, then practices seedling 1d to 2d.
10. the processing tomato immature seed according to claim 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 cultivates the method for seedling, it is characterized in that in the 3rd step, at room temperature cultivate 12d to 15d, then remove nutritive cube and training seedling is carried out normal management together with seedling medium transplanting to greenhouse or land for growing field crops; The first day nutritive cube transparent plastic bag sealing of at room temperature cultivating is cultivated, and within second day, opens osculum gradually, removes transparent plastic bag after the 6th day.
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| CN201410639363.XA CN104429486A (en) | 2014-11-13 | 2014-11-13 | Method for culturing immature seeds of processed tomatoes into seedlings |
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| CN201410639363.XA CN104429486A (en) | 2014-11-13 | 2014-11-13 | Method for culturing immature seeds of processed tomatoes into seedlings |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104838761A (en) * | 2015-05-20 | 2015-08-19 | 韩传起 | Tomato breeding method with high survival rate |
| CN105067482A (en) * | 2015-08-12 | 2015-11-18 | 中粮屯河种业有限公司 | Method for rapidly identifying high-viscosity tomato variety |
| CN110122212A (en) * | 2019-05-29 | 2019-08-16 | 武汉楚为生物科技股份有限公司 | The method of tomato Acceleration of generation rapid breeding |
| CN112106609A (en) * | 2020-10-12 | 2020-12-22 | 北京大学现代农业研究院 | Method for accelerating tomato breeding process |
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| JPH10313717A (en) * | 1997-05-15 | 1998-12-02 | Nikka Chem Co Ltd | Tomato seed and seedling culturing method |
| CN1650697A (en) * | 2005-02-24 | 2005-08-10 | 上海交通大学 | Fast culturing method of tomato homozygote |
| CN102187811A (en) * | 2011-03-21 | 2011-09-21 | 厦门华侨亚热带植物引种园 | Tomato culture medium and tomato culture method |
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2014
- 2014-11-13 CN CN201410639363.XA patent/CN104429486A/en active Pending
Patent Citations (4)
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| JPH10215717A (en) * | 1997-02-03 | 1998-08-18 | Nikka Chem Co Ltd | Culture method for tomato seed and seedling |
| JPH10313717A (en) * | 1997-05-15 | 1998-12-02 | Nikka Chem Co Ltd | Tomato seed and seedling culturing method |
| CN1650697A (en) * | 2005-02-24 | 2005-08-10 | 上海交通大学 | Fast culturing method of tomato homozygote |
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Non-Patent Citations (1)
| Title |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104838761A (en) * | 2015-05-20 | 2015-08-19 | 韩传起 | Tomato breeding method with high survival rate |
| CN105067482A (en) * | 2015-08-12 | 2015-11-18 | 中粮屯河种业有限公司 | Method for rapidly identifying high-viscosity tomato variety |
| CN110122212A (en) * | 2019-05-29 | 2019-08-16 | 武汉楚为生物科技股份有限公司 | The method of tomato Acceleration of generation rapid breeding |
| CN112106609A (en) * | 2020-10-12 | 2020-12-22 | 北京大学现代农业研究院 | Method for accelerating tomato breeding process |
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