Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of grafting method of macadamia nut seed bud, improve the survival rate of macadamia nut grafting, shorten nursery stage, reduce cultivating seedlings cost, make graft survival rate not by the impact in season, and the grafting of seed bud only needs semi-lignified branch, scion abundance, is applicable to large-scale industrialized nursery, for the great development of macadamia nut industry provides the guarantee of high quality seedling.
For achieving the above object, the present invention adopts following technical scheme:
A grafting method for macadamia nut seed bud, comprises the steps:
Step (1), presprouting of seeds: the seed cultivating stock is taken out from freezer, be placed in shady and cool local stacking 20-30 hour, then bask seeds 2-3 hour in the sun, afterwards seed is dipped in 20-30 hour in water, the seed of removing floating on water surface, goes out tiling to vernalization in constant temperature and humidity room, obtains the seed that season crack width reaches 1.5-2mm by remaining seed flood;
Step (2), cultivate stock: dig kind of a plant hole in nutritious bag matrix center, the specification of kind of plant hole is diameter × be 3 × 5cm deeply, the season crack width obtained in step (1) is reached the opening down sowing of seed of 1.5-2mm, after planting seed, water permeable, then nutritious bag is positioned in plastic tunnel cultivates 20-30 days, obtain the stock of bronze phase;
Step (3), gather scion: clip improved seeds elite stand then raw semi-lignified, healthy branch Zuo is Miho bar, and fringe bar is put into clear water immediately and soaked after cutting, take out drying water after soaking completely, load in moisture-keeping container immediately afterwards, be positioned over shady and cool place and preserve;
Step (4), cut scion: the scion that step (3) is obtained from branch base portion, upwards leaf-cutting successively, every wheel blade retains 1 blade, is then whittled into the short fringe that a fringe one takes turns leaf bud; Bevel must ensure smooth, smooth, clean, obtains fringe bar to be grafted;
Step (5), process scion: the fringe bar to be grafted step (4) obtained is immersed in 9-11 minute in disinfectant, after be immersed in nutrient solution, soak time more than 1 hour, can not obtain grafting scion; Described nutrient solution comprises following component: methyl α-naphthyl acetate 1g, indolebutyric acid 1g, many receipts liquid 30g, 1 liter, water;
Step (6), cut anvil seedling: bronze phase stock step (2) obtained cuts off at distance Nutrition Soil 3cm place, the part of removing more than 3cm, and then from the downward partial application in the centre of otch plane, incision length 2cm, obtains graft stock otch;
Step (7), grafting: the graft stock otch that the grafting scion inserting step (6) step (5) obtained obtains, grafting , Shi Miho bar and stock are combined closely;
Step (8), except rudiment, hardening: 11-13 days grafting mouths after grafting start healing, at this moment need to open greenhouse film transom 1-2 hour every day, the rudiment that stock of erasing in time grows; Miho bar erases unnecessary branch after germinateing, and stays next branch to cultivate as major branch;
Step (9), truncation is done surely: truncation, and after truncation, scion takes out the tip more than 2 times, and the blade of the 2nd tip is aging can go out garden field planting.
Further, preferably in step (1), the temperature of vernalization is 33-35 DEG C, and humidity is 85-95%.
Further, the specification of preferably planting plant hole in step (2) is diameter × be 3 × 5cm deeply.
Further, preferably described in step (4) to be whittled into the method that a fringe one takes turns the short fringe of leaf bud be scion be held between left hand thumb and middle finger, one side is close in forefinger tip, a cutter of splaying at distance 4-5cm place of bastem portion, and inclined-plane is at 45 °; Overturn that scion is flat cuts a cutter again, deeply reach xylem, the long 1.5-2cm of bevel, the finally oblique partial application in 1cm place above bud point, cut off scion, to obtain final product.Being whittled into the method that a fringe one takes turns the short fringe of leaf bud is not that this is a kind of, as long as can achieve the goal.
Further, the grafting mouth of the preferably grafting described in step (7) needs to clamp with Grafting clip.
Further, the temperature of 25-32 DEG C to preferably be kept in step (8) after grafting in booth, the humidity of more than 95%.
Further, preferably the middle truncation of step (9) is cultivate major branch at height of seedling 50cm place truncation.
compared with prior art, its beneficial effect is in the present invention:(1) the present invention adopts the semi-lignified branch of band 1 blade as scion, the nutritious bag bronze phase seed bud seedling of stock normal growth, seedling age 2 weeks, adopts cleft graft grafting, plants cultivating and managing under booth after grafting; (2) the inventive method vernalization in constant temperature and humidity room, removes from and builds the husky bed of vernalization, save a large amount of human and material resources; (3) stock is cultivated in plastic tunnel, the enforcement of convenient management and grafting; (4) the present invention adopts semi-lignified branch to do scion and the grafting of nutritious bag bronze phase seed bud seedling phase, due to stock and scion degree of lignification all low, therefore histocyte enlivens, form callus very fast, the blade retained can carry out photosynthesis and manufacture the healing of nutriment supply grafting mouth, makes graft survival rate not by the impact in season.So than traditional autumn, stiff wood grafting survival rate raising in spring 25%, graft survival rate is up to more than 95%, (4) seed bud grafting cultivates out garden from stock only needs 10 months, and traditional autumn, stiff wood grafting nursery in spring, owing to cultivating time restriction by stock, the impact in grafting season, cultivate out garden from stock and need 18-30 month, so use seed bud graft technology, greatly improve the survival rate of macadamia nut grafting, shorten nursery stage, reduce cultivating seedlings cost, make graft survival rate not by seasonal effect, as long as and the grafting of seed bud has semi-lignified branch, throughout the year all can grafting, save great Liang Miho bar, be applicable to large-scale industrialized nursery, produce upper a large amount of seedling for macadamia nut to provide safeguard.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
It will be understood to those of skill in the art that the following example only for illustration of the present invention, and should not be considered as limiting scope of the present invention.Unreceipted concrete technology or condition person in embodiment, according to the technology described by the document in this area or condition or carry out according to product description.Agents useful for same or the unreceipted production firm person of instrument, being can by buying the conventional products obtained.
Embodiment 1
A grafting method for macadamia nut seed bud, comprises the steps:
Step (1), presprouting of seeds: the seed cultivating stock is taken out from freezer, be placed in shady and cool local stacking 20 hours, then bask seeds in the sun 2 hours, afterwards seed to be dipped in water 20 hours, the seed of removing floating on water surface, it is 33 DEG C that remaining seed flood is gone out tiling to temperature, humidity is vernalization in the constant temperature and humidity room of 85-88%, obtains the seed that season crack width reaches 1.5-2mm;
Step (2), cultivate stock: dig kind of a plant hole in nutritious bag matrix center, the specification of kind of plant hole is diameter × be 3 × 5cm deeply, the season crack width obtained in step (1) is reached the opening down sowing of seed of 1.5-2mm, after planting seed, water permeable, then nutritious bag is positioned in plastic tunnel cultivate 20 days, obtain the stock of bronze phase;
Step (3), gather scion: clip improved seeds elite stand then raw semi-lignified, healthy branch Zuo is Miho bar, and fringe bar is put into clear water immediately and soaked after cutting, take out drying water after soaking completely, load in moisture-keeping container immediately afterwards, be positioned over shady and cool place and preserve;
Step (4), cut scion: the scion that step (3) is obtained from branch base portion, upwards leaf-cutting successively, every wheel blade retains 1 blade, is then whittled into the short fringe that a fringe one takes turns leaf bud; Bevel must ensure smooth, smooth, clean, obtains fringe bar to be grafted;
Step (5), process scion: the fringe bar to be grafted step (4) obtained to be immersed in disinfectant 9 minutes, after be immersed in nutrient solution, soak time more than 1 hour, can not obtain grafting scion; Described nutrient solution comprises following component: methyl α-naphthyl acetate 1g, indolebutyric acid 1g, many receipts liquid 30g, 1 liter, water;
Step (6), cut anvil seedling: bronze phase stock step (2) obtained cuts off at distance Nutrition Soil 3cm place, the part of removing more than 3cm, and then from the downward partial application in the centre of otch plane, incision length 2cm, obtains graft stock otch;
Step (7), grafting: the graft stock otch that the grafting scion inserting step (6) step (5) obtained obtains, grafting, Grafting clip clamping grafting Kou Shi Miho bar and stock are combined closely;
Step (8), except rudiment, hardening: the temperature that will keep 25-27 DEG C after grafting in booth, the humidity of more than 95%, within 11st day, grafting mouth starts healing, at this moment need to open greenhouse film transom every day 1 hour, the rudiment that stock of erasing in time grows, Miho bar erases unnecessary branch after germinateing, and stays next branch to cultivate as major branch;
Step (9), truncation is done surely: cultivate major branch at height of seedling 50cm place truncation, after truncation, scion takes out the tip more than 2 times, and the blade of the 2nd tip is aging can go out garden field planting.
The graft survival rate of the present embodiment is 95%.
Embodiment 2
A grafting method for macadamia nut seed bud, comprises the steps:
Step (1), presprouting of seeds: the seed cultivating stock is taken out from freezer, be placed in shady and cool local stacking 30 hours, then bask seeds in the sun 3 hours, afterwards seed to be dipped in water 30 hours, the seed of removing floating on water surface, it is 35 DEG C that remaining seed flood is gone out tiling to temperature, humidity is vernalization in the constant temperature and humidity room of 92-95%, obtains the seed that season crack width reaches 1.5-2mm;
Step (2), cultivate stock: dig kind of a plant hole in nutritious bag matrix center, the specification of kind of plant hole is diameter × be 3 × 5cm deeply, the season crack width obtained in step (1) is reached the opening down sowing of seed of 1.5-2mm, after planting seed, water permeable, then nutritious bag is positioned in plastic tunnel cultivate 25 days, obtain the stock of bronze phase;
Step (3), gather scion: clip improved seeds elite stand then raw semi-lignified, healthy branch Zuo is Miho bar, and fringe bar is put into clear water immediately and soaked after cutting, take out drying water after soaking completely, load in moisture-keeping container immediately afterwards, be positioned over shady and cool place and preserve;
Step (4), cut scion: the scion that step (3) is obtained from branch base portion, upwards leaf-cutting successively, every wheel blade retains 1 blade, is then whittled into the short fringe that a fringe one takes turns leaf bud; Bevel must ensure smooth, smooth, clean, obtains fringe bar to be grafted;
Step (5), process scion: the fringe bar to be grafted step (4) obtained to be immersed in disinfectant 11 minutes, after be immersed in nutrient solution, soak time more than 1 hour, can not obtain grafting scion; Described nutrient solution comprises following component: methyl α-naphthyl acetate 1g, indolebutyric acid 1g, many receipts liquid 30g, 1 liter, water;
Step (6), cut anvil seedling: bronze phase stock step (2) obtained cuts off at distance Nutrition Soil 3cm place, the part of removing more than 3cm, and then from the downward partial application in the centre of otch plane, incision length 2cm, obtains graft stock otch;
Step (7), grafting: the graft stock otch that the grafting scion inserting step (6) step (5) obtained obtains, grafting, Grafting clip clamping grafting Kou Shi Miho bar and stock are combined closely;
Step (8), except rudiment, hardening: the temperature that will keep 30-32 DEG C after grafting in booth, the humidity of more than 95%, within 13rd day, grafting mouth starts healing, at this moment need to open greenhouse film transom every day 2 hours, the rudiment that stock of erasing in time grows, Miho bar erases unnecessary branch after germinateing, and stays next branch to cultivate as major branch;
Step (9), truncation is done surely: cultivate major branch at height of seedling 50cm place truncation, after truncation, scion takes out the tip more than 2 times, and the blade of the 2nd tip is aging can go out garden field planting.
Described to be whittled into the method that a fringe one takes turns the short fringe of leaf bud be scion be held between left hand thumb and middle finger, and one side is close in forefinger tip, a cutter of splaying at distance 4cm place of bastem portion, and inclined-plane is at 45 °; Overturn that scion is flat cuts a cutter again, deeply reach xylem, the long 1.5cm of bevel, the finally oblique partial application in 1cm place above bud point, cut off scion, to obtain final product.
The graft survival rate of the present embodiment is 97%.
Embodiment 3
A grafting method for macadamia nut seed bud, comprises the steps:
Step (1), presprouting of seeds: the seed cultivating stock is taken out from freezer, be placed in shady and cool local stacking 25 hours, then bask seeds in the sun 2.3 hours, afterwards seed to be dipped in water 22 hours, the seed of removing floating on water surface, it is 34 DEG C that remaining seed flood is gone out tiling to temperature, humidity is vernalization in the constant temperature and humidity room of 90%, obtains the seed that season crack width reaches 1.5-2mm;
Step (2), cultivate stock: dig kind of a plant hole in nutritious bag matrix center, the specification of kind of plant hole is diameter × be 3 × 5cm deeply, the season crack width obtained in step (1) is reached the opening down sowing of seed of 1.5-2mm, after planting seed, water permeable, then nutritious bag is positioned in plastic tunnel cultivate 28 days, obtain the stock of bronze phase;
Step (3), gather scion: clip improved seeds elite stand then raw semi-lignified, healthy branch Zuo is Miho bar, and fringe bar is put into clear water immediately and soaked after cutting, take out drying water after soaking completely, load in moisture-keeping container immediately afterwards, be positioned over shady and cool place and preserve;
Step (4), cut scion: the scion that step (3) is obtained from branch base portion, upwards leaf-cutting successively, every wheel blade retains 1 blade, is then whittled into the short fringe that a fringe one takes turns leaf bud; Bevel must ensure smooth, smooth, clean, obtains fringe bar to be grafted;
Step (5), process scion: the fringe bar to be grafted step (4) obtained to be immersed in disinfectant 10 minutes, after be immersed in nutrient solution, soak time more than 1 hour, can not obtain grafting scion; Described nutrient solution comprises following component: methyl α-naphthyl acetate 1g, indolebutyric acid 1g, many receipts liquid 30g, 1 liter, water;
Step (6), cut anvil seedling: bronze phase stock step (2) obtained cuts off at distance Nutrition Soil 3cm place, the part of removing more than 3cm, and then from the downward partial application in the centre of otch plane, incision length 2cm, obtains graft stock otch;
Step (7), grafting: the graft stock otch that the grafting scion inserting step (6) step (5) obtained obtains, grafting, Grafting clip clamping grafting Kou Shi Miho bar and stock are combined closely;
Step (8), except rudiment, hardening: the temperature that will keep 28-30 DEG C after grafting in booth, the humidity of more than 95%, within 12nd day, grafting mouth starts healing, at this moment need to open greenhouse film transom every day 1.2 hours, the rudiment that stock of erasing in time grows, Miho bar erases unnecessary branch after germinateing, and stays next branch to cultivate as major branch;
Step (9), truncation is done surely: cultivate major branch at height of seedling 50cm place truncation, after truncation, scion takes out the tip more than 2 times, and the blade of the 2nd tip is aging can go out garden field planting.
Described to be whittled into the method that a fringe one takes turns the short fringe of leaf bud be scion be held between left hand thumb and middle finger, and one side is close in forefinger tip, a cutter of splaying at distance 5cm place of bastem portion, and inclined-plane is at 45 °; Overturn that scion is flat cuts a cutter again, deeply reach xylem, the long 2cm of bevel, the finally oblique partial application in 1cm place above bud point, cut off scion, to obtain final product.
The graft survival rate of the present embodiment is 98.5%.
Embodiment 4
A grafting method for macadamia nut seed bud, comprises the steps:
Step (1), presprouting of seeds: the seed cultivating stock is taken out from freezer, be placed in shady and cool local stacking 26 hours, then bask seeds in the sun 2.8 hours, afterwards seed to be dipped in water 27 hours, the seed of removing floating on water surface, it is 34 DEG C that remaining seed flood is gone out tiling to temperature, humidity is vernalization in the constant temperature and humidity room of 88%, obtains the seed that season crack width reaches 1.5-2mm;
Step (2), cultivate stock: dig kind of a plant hole in nutritious bag matrix center, the specification of kind of plant hole is diameter × be 3 × 5cm deeply, the season crack width obtained in step (1) is reached the opening down sowing of seed of 1.5-2mm, after planting seed, water permeable, then nutritious bag is positioned in plastic tunnel cultivate 24 days, obtain the stock of bronze phase;
Step (3), gather scion: clip improved seeds elite stand then raw semi-lignified, healthy branch Zuo is Miho bar, and fringe bar is put into clear water immediately and soaked after cutting, take out drying water after soaking completely, load in moisture-keeping container immediately afterwards, be positioned over shady and cool place and preserve;
Step (4), cut scion: the scion that step (3) is obtained from branch base portion, upwards leaf-cutting successively, every wheel blade retains 1 blade, is then whittled into the short fringe that a fringe one takes turns leaf bud; Bevel must ensure smooth, smooth, clean, obtains fringe bar to be grafted;
Step (5), process scion: the fringe bar to be grafted step (4) obtained to be immersed in disinfectant 10 minutes, after be immersed in nutrient solution, soak time more than 1 hour, can not obtain grafting scion; Described nutrient solution comprises following component: methyl α-naphthyl acetate 1g, indolebutyric acid 1g, many receipts liquid 30g, 1 liter, water;
Step (6), cut anvil seedling: bronze phase stock step (2) obtained cuts off at distance Nutrition Soil 3cm place, the part of removing more than 3cm, and then from the downward partial application in the centre of otch plane, incision length 2cm, obtains graft stock otch;
Step (7), grafting: the graft stock otch that the grafting scion inserting step (6) step (5) obtained obtains, grafting, Grafting clip clamping grafting Kou Shi Miho bar and stock are combined closely;
Step (8), except rudiment, hardening: the temperature that will keep 29-31 DEG C after grafting in booth, the humidity of more than 95%, within 12nd day, grafting mouth starts healing, at this moment need to open greenhouse film transom every day 1.8 hours, the rudiment that stock of erasing in time grows, Miho bar erases unnecessary branch after germinateing, and stays next branch to cultivate as major branch;
Step (9), truncation is done surely: cultivate major branch at height of seedling 50cm place truncation, after truncation, scion takes out the tip more than 2 times, and the blade of the 2nd tip is aging can go out garden field planting.
Described to be whittled into the method that a fringe one takes turns the short fringe of leaf bud be scion be held between left hand thumb and middle finger, and one side is close in forefinger tip, a cutter of splaying at distance 4.5m place of bastem portion, and inclined-plane is at 45 °; Overturn that scion is flat cuts a cutter again, deeply reach xylem, the long 1.8cm of bevel, the finally oblique partial application in 1cm place above bud point, cut off scion, to obtain final product.
The graft survival rate of the present embodiment is 99%.
More than show and describe general principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof
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