CN104488576A - Grafting method of macadimia nut sprout - Google Patents

Grafting method of macadimia nut sprout Download PDF

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Publication number
CN104488576A
CN104488576A CN201510002467.4A CN201510002467A CN104488576A CN 104488576 A CN104488576 A CN 104488576A CN 201510002467 A CN201510002467 A CN 201510002467A CN 104488576 A CN104488576 A CN 104488576A
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grafting
scion
stock
seed
leaf
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CN104488576B (en
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徐斌
吴云翔
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Jiangcheng Jinao Biotechnology Co., Ltd
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JIANGCHENG ZHONGAO AGRICULTURE TECHNOLOGY DEVELOPMENT Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/30Grafting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G3/00Cutting implements specially adapted for horticultural purposes; Delimbing standing trees
    • A01G2003/005Removing buds

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention relates to a grafting method of a macadimia nut sprout, and belongs to the technical field of agriculture and forestry. The method is that a semi-lignified branch with one leaf is used as a cion, the seed sprout at the age of two weeks and normally glowing at leaf-expansion period through a nutrition bag is used as a stock, then the split grafting method is performed for grafting, and the sprout is transplanted in a greenhouse and subjected to culture management. The method has the advantages that the semi-lignified branch with one leaf is used as the cion is grafted to the seed sprout at the leaf-expansion period in the nutrition bag; the stock and cion are both in low degree of lignifications, so that the histocyte is active, callus can be generated fast, and the remained leaf is able to perform photosynthesis to produce nutrient substances for curing the grafting opening; by being compared with traditional hard-twig grafting in autumn and spring, the grafting method has the advantages that survive rate is raised by 25% and the grafting survive rate exceeds 95%; in addition, the whole process from sprout grafting, culturing of stock to out-planting needs about 10 months, so that mass industrial seedling applies.

Description

A kind of grafting method of macadamia nut seed bud
Technical field
The invention belongs to agricultural technical field, be specifically related to a kind of grafting method of macadamia nut seed bud.
Background technology
Macadamia nut ( macadamiaspp.), originating in the hylaeion hypotropicum of the Queensland, Australia southeast and New South Wales's north Coast, is perennial evergreen standard fruit-tree, is the good emerging fruit tree of a kind of development prospect.Macadamia nut is nutritious, economic worth is high, is five-star one in world's nut fruits food.Current main product is in states such as Australia, South Africa, the U.S. and Kenya, and the cultivated area of China's macadamia nut occupies the first in the world.Macadamia nut, because branch formation layer is very thin, being difficult to grafting survival, is one of successful fruit tree of the most difficult grafting of generally acknowledging in the world.
Have data to show, in country variant and different ecological Suitable Area, there is larger difference in its seedling raising manners and graft technology etc.Traditional raising seedling of Australian nut method is when the liftoff 20-30cm place of stock, when stem slightly reaches 0.7-1.2cm, can carry out grafting.Meet the stock of this condition, at least will grow more than 12 months, in well-regulated situation, within after grafting 6 months, can garden be gone out.With the macadamia nut grafting that conventional method is cultivated, from being seeded into out garden, generally take 18-30 month, under the prerequisite that, grafting time good in grafting work skilled operation, management is applicable to, graft survival rate is generally about 70%, and it is long that visible traditional seedling-cultivating method exists growing-seedling period, the problems such as production cost is high, therefore, innovating macadamia nut seedling fostering technology or improve, is the problem needing solution at present badly.
Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of grafting method of macadamia nut seed bud, improve the survival rate of macadamia nut grafting, shorten nursery stage, reduce cultivating seedlings cost, make graft survival rate not by the impact in season, and the grafting of seed bud only needs semi-lignified branch, scion abundance, is applicable to large-scale industrialized nursery, for the great development of macadamia nut industry provides the guarantee of high quality seedling.
For achieving the above object, the present invention adopts following technical scheme:
A grafting method for macadamia nut seed bud, comprises the steps:
Step (1), presprouting of seeds: the seed cultivating stock is taken out from freezer, be placed in shady and cool local stacking 20-30 hour, then bask seeds 2-3 hour in the sun, afterwards seed is dipped in 20-30 hour in water, the seed of removing floating on water surface, goes out tiling to vernalization in constant temperature and humidity room, obtains the seed that season crack width reaches 1.5-2mm by remaining seed flood;
Step (2), cultivate stock: dig kind of a plant hole in nutritious bag matrix center, the specification of kind of plant hole is diameter × be 3 × 5cm deeply, the season crack width obtained in step (1) is reached the opening down sowing of seed of 1.5-2mm, after planting seed, water permeable, then nutritious bag is positioned in plastic tunnel cultivates 20-30 days, obtain the stock of bronze phase;
Step (3), gather scion: clip improved seeds elite stand then raw semi-lignified, healthy branch Zuo is Miho bar, and fringe bar is put into clear water immediately and soaked after cutting, take out drying water after soaking completely, load in moisture-keeping container immediately afterwards, be positioned over shady and cool place and preserve;
Step (4), cut scion: the scion that step (3) is obtained from branch base portion, upwards leaf-cutting successively, every wheel blade retains 1 blade, is then whittled into the short fringe that a fringe one takes turns leaf bud; Bevel must ensure smooth, smooth, clean, obtains fringe bar to be grafted;
Step (5), process scion: the fringe bar to be grafted step (4) obtained is immersed in 9-11 minute in disinfectant, after be immersed in nutrient solution, soak time more than 1 hour, can not obtain grafting scion; Described nutrient solution comprises following component: methyl α-naphthyl acetate 1g, indolebutyric acid 1g, many receipts liquid 30g, 1 liter, water;
Step (6), cut anvil seedling: bronze phase stock step (2) obtained cuts off at distance Nutrition Soil 3cm place, the part of removing more than 3cm, and then from the downward partial application in the centre of otch plane, incision length 2cm, obtains graft stock otch;
Step (7), grafting: the graft stock otch that the grafting scion inserting step (6) step (5) obtained obtains, grafting , Shi Miho bar and stock are combined closely;
Step (8), except rudiment, hardening: 11-13 days grafting mouths after grafting start healing, at this moment need to open greenhouse film transom 1-2 hour every day, the rudiment that stock of erasing in time grows; Miho bar erases unnecessary branch after germinateing, and stays next branch to cultivate as major branch;
Step (9), truncation is done surely: truncation, and after truncation, scion takes out the tip more than 2 times, and the blade of the 2nd tip is aging can go out garden field planting.
Further, preferably in step (1), the temperature of vernalization is 33-35 DEG C, and humidity is 85-95%.
Further, the specification of preferably planting plant hole in step (2) is diameter × be 3 × 5cm deeply.
Further, preferably described in step (4) to be whittled into the method that a fringe one takes turns the short fringe of leaf bud be scion be held between left hand thumb and middle finger, one side is close in forefinger tip, a cutter of splaying at distance 4-5cm place of bastem portion, and inclined-plane is at 45 °; Overturn that scion is flat cuts a cutter again, deeply reach xylem, the long 1.5-2cm of bevel, the finally oblique partial application in 1cm place above bud point, cut off scion, to obtain final product.Being whittled into the method that a fringe one takes turns the short fringe of leaf bud is not that this is a kind of, as long as can achieve the goal.
Further, the grafting mouth of the preferably grafting described in step (7) needs to clamp with Grafting clip.
Further, the temperature of 25-32 DEG C to preferably be kept in step (8) after grafting in booth, the humidity of more than 95%.
Further, preferably the middle truncation of step (9) is cultivate major branch at height of seedling 50cm place truncation.
compared with prior art, its beneficial effect is in the present invention:(1) the present invention adopts the semi-lignified branch of band 1 blade as scion, the nutritious bag bronze phase seed bud seedling of stock normal growth, seedling age 2 weeks, adopts cleft graft grafting, plants cultivating and managing under booth after grafting; (2) the inventive method vernalization in constant temperature and humidity room, removes from and builds the husky bed of vernalization, save a large amount of human and material resources; (3) stock is cultivated in plastic tunnel, the enforcement of convenient management and grafting; (4) the present invention adopts semi-lignified branch to do scion and the grafting of nutritious bag bronze phase seed bud seedling phase, due to stock and scion degree of lignification all low, therefore histocyte enlivens, form callus very fast, the blade retained can carry out photosynthesis and manufacture the healing of nutriment supply grafting mouth, makes graft survival rate not by the impact in season.So than traditional autumn, stiff wood grafting survival rate raising in spring 25%, graft survival rate is up to more than 95%, (4) seed bud grafting cultivates out garden from stock only needs 10 months, and traditional autumn, stiff wood grafting nursery in spring, owing to cultivating time restriction by stock, the impact in grafting season, cultivate out garden from stock and need 18-30 month, so use seed bud graft technology, greatly improve the survival rate of macadamia nut grafting, shorten nursery stage, reduce cultivating seedlings cost, make graft survival rate not by seasonal effect, as long as and the grafting of seed bud has semi-lignified branch, throughout the year all can grafting, save great Liang Miho bar, be applicable to large-scale industrialized nursery, produce upper a large amount of seedling for macadamia nut to provide safeguard.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
It will be understood to those of skill in the art that the following example only for illustration of the present invention, and should not be considered as limiting scope of the present invention.Unreceipted concrete technology or condition person in embodiment, according to the technology described by the document in this area or condition or carry out according to product description.Agents useful for same or the unreceipted production firm person of instrument, being can by buying the conventional products obtained.
Embodiment 1
A grafting method for macadamia nut seed bud, comprises the steps:
Step (1), presprouting of seeds: the seed cultivating stock is taken out from freezer, be placed in shady and cool local stacking 20 hours, then bask seeds in the sun 2 hours, afterwards seed to be dipped in water 20 hours, the seed of removing floating on water surface, it is 33 DEG C that remaining seed flood is gone out tiling to temperature, humidity is vernalization in the constant temperature and humidity room of 85-88%, obtains the seed that season crack width reaches 1.5-2mm;
Step (2), cultivate stock: dig kind of a plant hole in nutritious bag matrix center, the specification of kind of plant hole is diameter × be 3 × 5cm deeply, the season crack width obtained in step (1) is reached the opening down sowing of seed of 1.5-2mm, after planting seed, water permeable, then nutritious bag is positioned in plastic tunnel cultivate 20 days, obtain the stock of bronze phase;
Step (3), gather scion: clip improved seeds elite stand then raw semi-lignified, healthy branch Zuo is Miho bar, and fringe bar is put into clear water immediately and soaked after cutting, take out drying water after soaking completely, load in moisture-keeping container immediately afterwards, be positioned over shady and cool place and preserve;
Step (4), cut scion: the scion that step (3) is obtained from branch base portion, upwards leaf-cutting successively, every wheel blade retains 1 blade, is then whittled into the short fringe that a fringe one takes turns leaf bud; Bevel must ensure smooth, smooth, clean, obtains fringe bar to be grafted;
Step (5), process scion: the fringe bar to be grafted step (4) obtained to be immersed in disinfectant 9 minutes, after be immersed in nutrient solution, soak time more than 1 hour, can not obtain grafting scion; Described nutrient solution comprises following component: methyl α-naphthyl acetate 1g, indolebutyric acid 1g, many receipts liquid 30g, 1 liter, water;
Step (6), cut anvil seedling: bronze phase stock step (2) obtained cuts off at distance Nutrition Soil 3cm place, the part of removing more than 3cm, and then from the downward partial application in the centre of otch plane, incision length 2cm, obtains graft stock otch;
Step (7), grafting: the graft stock otch that the grafting scion inserting step (6) step (5) obtained obtains, grafting, Grafting clip clamping grafting Kou Shi Miho bar and stock are combined closely;
Step (8), except rudiment, hardening: the temperature that will keep 25-27 DEG C after grafting in booth, the humidity of more than 95%, within 11st day, grafting mouth starts healing, at this moment need to open greenhouse film transom every day 1 hour, the rudiment that stock of erasing in time grows, Miho bar erases unnecessary branch after germinateing, and stays next branch to cultivate as major branch;
Step (9), truncation is done surely: cultivate major branch at height of seedling 50cm place truncation, after truncation, scion takes out the tip more than 2 times, and the blade of the 2nd tip is aging can go out garden field planting.
The graft survival rate of the present embodiment is 95%.
Embodiment 2
A grafting method for macadamia nut seed bud, comprises the steps:
Step (1), presprouting of seeds: the seed cultivating stock is taken out from freezer, be placed in shady and cool local stacking 30 hours, then bask seeds in the sun 3 hours, afterwards seed to be dipped in water 30 hours, the seed of removing floating on water surface, it is 35 DEG C that remaining seed flood is gone out tiling to temperature, humidity is vernalization in the constant temperature and humidity room of 92-95%, obtains the seed that season crack width reaches 1.5-2mm;
Step (2), cultivate stock: dig kind of a plant hole in nutritious bag matrix center, the specification of kind of plant hole is diameter × be 3 × 5cm deeply, the season crack width obtained in step (1) is reached the opening down sowing of seed of 1.5-2mm, after planting seed, water permeable, then nutritious bag is positioned in plastic tunnel cultivate 25 days, obtain the stock of bronze phase;
Step (3), gather scion: clip improved seeds elite stand then raw semi-lignified, healthy branch Zuo is Miho bar, and fringe bar is put into clear water immediately and soaked after cutting, take out drying water after soaking completely, load in moisture-keeping container immediately afterwards, be positioned over shady and cool place and preserve;
Step (4), cut scion: the scion that step (3) is obtained from branch base portion, upwards leaf-cutting successively, every wheel blade retains 1 blade, is then whittled into the short fringe that a fringe one takes turns leaf bud; Bevel must ensure smooth, smooth, clean, obtains fringe bar to be grafted;
Step (5), process scion: the fringe bar to be grafted step (4) obtained to be immersed in disinfectant 11 minutes, after be immersed in nutrient solution, soak time more than 1 hour, can not obtain grafting scion; Described nutrient solution comprises following component: methyl α-naphthyl acetate 1g, indolebutyric acid 1g, many receipts liquid 30g, 1 liter, water;
Step (6), cut anvil seedling: bronze phase stock step (2) obtained cuts off at distance Nutrition Soil 3cm place, the part of removing more than 3cm, and then from the downward partial application in the centre of otch plane, incision length 2cm, obtains graft stock otch;
Step (7), grafting: the graft stock otch that the grafting scion inserting step (6) step (5) obtained obtains, grafting, Grafting clip clamping grafting Kou Shi Miho bar and stock are combined closely;
Step (8), except rudiment, hardening: the temperature that will keep 30-32 DEG C after grafting in booth, the humidity of more than 95%, within 13rd day, grafting mouth starts healing, at this moment need to open greenhouse film transom every day 2 hours, the rudiment that stock of erasing in time grows, Miho bar erases unnecessary branch after germinateing, and stays next branch to cultivate as major branch;
Step (9), truncation is done surely: cultivate major branch at height of seedling 50cm place truncation, after truncation, scion takes out the tip more than 2 times, and the blade of the 2nd tip is aging can go out garden field planting.
Described to be whittled into the method that a fringe one takes turns the short fringe of leaf bud be scion be held between left hand thumb and middle finger, and one side is close in forefinger tip, a cutter of splaying at distance 4cm place of bastem portion, and inclined-plane is at 45 °; Overturn that scion is flat cuts a cutter again, deeply reach xylem, the long 1.5cm of bevel, the finally oblique partial application in 1cm place above bud point, cut off scion, to obtain final product.
The graft survival rate of the present embodiment is 97%.
Embodiment 3
A grafting method for macadamia nut seed bud, comprises the steps:
Step (1), presprouting of seeds: the seed cultivating stock is taken out from freezer, be placed in shady and cool local stacking 25 hours, then bask seeds in the sun 2.3 hours, afterwards seed to be dipped in water 22 hours, the seed of removing floating on water surface, it is 34 DEG C that remaining seed flood is gone out tiling to temperature, humidity is vernalization in the constant temperature and humidity room of 90%, obtains the seed that season crack width reaches 1.5-2mm;
Step (2), cultivate stock: dig kind of a plant hole in nutritious bag matrix center, the specification of kind of plant hole is diameter × be 3 × 5cm deeply, the season crack width obtained in step (1) is reached the opening down sowing of seed of 1.5-2mm, after planting seed, water permeable, then nutritious bag is positioned in plastic tunnel cultivate 28 days, obtain the stock of bronze phase;
Step (3), gather scion: clip improved seeds elite stand then raw semi-lignified, healthy branch Zuo is Miho bar, and fringe bar is put into clear water immediately and soaked after cutting, take out drying water after soaking completely, load in moisture-keeping container immediately afterwards, be positioned over shady and cool place and preserve;
Step (4), cut scion: the scion that step (3) is obtained from branch base portion, upwards leaf-cutting successively, every wheel blade retains 1 blade, is then whittled into the short fringe that a fringe one takes turns leaf bud; Bevel must ensure smooth, smooth, clean, obtains fringe bar to be grafted;
Step (5), process scion: the fringe bar to be grafted step (4) obtained to be immersed in disinfectant 10 minutes, after be immersed in nutrient solution, soak time more than 1 hour, can not obtain grafting scion; Described nutrient solution comprises following component: methyl α-naphthyl acetate 1g, indolebutyric acid 1g, many receipts liquid 30g, 1 liter, water;
Step (6), cut anvil seedling: bronze phase stock step (2) obtained cuts off at distance Nutrition Soil 3cm place, the part of removing more than 3cm, and then from the downward partial application in the centre of otch plane, incision length 2cm, obtains graft stock otch;
Step (7), grafting: the graft stock otch that the grafting scion inserting step (6) step (5) obtained obtains, grafting, Grafting clip clamping grafting Kou Shi Miho bar and stock are combined closely;
Step (8), except rudiment, hardening: the temperature that will keep 28-30 DEG C after grafting in booth, the humidity of more than 95%, within 12nd day, grafting mouth starts healing, at this moment need to open greenhouse film transom every day 1.2 hours, the rudiment that stock of erasing in time grows, Miho bar erases unnecessary branch after germinateing, and stays next branch to cultivate as major branch;
Step (9), truncation is done surely: cultivate major branch at height of seedling 50cm place truncation, after truncation, scion takes out the tip more than 2 times, and the blade of the 2nd tip is aging can go out garden field planting.
Described to be whittled into the method that a fringe one takes turns the short fringe of leaf bud be scion be held between left hand thumb and middle finger, and one side is close in forefinger tip, a cutter of splaying at distance 5cm place of bastem portion, and inclined-plane is at 45 °; Overturn that scion is flat cuts a cutter again, deeply reach xylem, the long 2cm of bevel, the finally oblique partial application in 1cm place above bud point, cut off scion, to obtain final product.
The graft survival rate of the present embodiment is 98.5%.
Embodiment 4
A grafting method for macadamia nut seed bud, comprises the steps:
Step (1), presprouting of seeds: the seed cultivating stock is taken out from freezer, be placed in shady and cool local stacking 26 hours, then bask seeds in the sun 2.8 hours, afterwards seed to be dipped in water 27 hours, the seed of removing floating on water surface, it is 34 DEG C that remaining seed flood is gone out tiling to temperature, humidity is vernalization in the constant temperature and humidity room of 88%, obtains the seed that season crack width reaches 1.5-2mm;
Step (2), cultivate stock: dig kind of a plant hole in nutritious bag matrix center, the specification of kind of plant hole is diameter × be 3 × 5cm deeply, the season crack width obtained in step (1) is reached the opening down sowing of seed of 1.5-2mm, after planting seed, water permeable, then nutritious bag is positioned in plastic tunnel cultivate 24 days, obtain the stock of bronze phase;
Step (3), gather scion: clip improved seeds elite stand then raw semi-lignified, healthy branch Zuo is Miho bar, and fringe bar is put into clear water immediately and soaked after cutting, take out drying water after soaking completely, load in moisture-keeping container immediately afterwards, be positioned over shady and cool place and preserve;
Step (4), cut scion: the scion that step (3) is obtained from branch base portion, upwards leaf-cutting successively, every wheel blade retains 1 blade, is then whittled into the short fringe that a fringe one takes turns leaf bud; Bevel must ensure smooth, smooth, clean, obtains fringe bar to be grafted;
Step (5), process scion: the fringe bar to be grafted step (4) obtained to be immersed in disinfectant 10 minutes, after be immersed in nutrient solution, soak time more than 1 hour, can not obtain grafting scion; Described nutrient solution comprises following component: methyl α-naphthyl acetate 1g, indolebutyric acid 1g, many receipts liquid 30g, 1 liter, water;
Step (6), cut anvil seedling: bronze phase stock step (2) obtained cuts off at distance Nutrition Soil 3cm place, the part of removing more than 3cm, and then from the downward partial application in the centre of otch plane, incision length 2cm, obtains graft stock otch;
Step (7), grafting: the graft stock otch that the grafting scion inserting step (6) step (5) obtained obtains, grafting, Grafting clip clamping grafting Kou Shi Miho bar and stock are combined closely;
Step (8), except rudiment, hardening: the temperature that will keep 29-31 DEG C after grafting in booth, the humidity of more than 95%, within 12nd day, grafting mouth starts healing, at this moment need to open greenhouse film transom every day 1.8 hours, the rudiment that stock of erasing in time grows, Miho bar erases unnecessary branch after germinateing, and stays next branch to cultivate as major branch;
Step (9), truncation is done surely: cultivate major branch at height of seedling 50cm place truncation, after truncation, scion takes out the tip more than 2 times, and the blade of the 2nd tip is aging can go out garden field planting.
Described to be whittled into the method that a fringe one takes turns the short fringe of leaf bud be scion be held between left hand thumb and middle finger, and one side is close in forefinger tip, a cutter of splaying at distance 4.5m place of bastem portion, and inclined-plane is at 45 °; Overturn that scion is flat cuts a cutter again, deeply reach xylem, the long 1.8cm of bevel, the finally oblique partial application in 1cm place above bud point, cut off scion, to obtain final product.
The graft survival rate of the present embodiment is 99%.
More than show and describe general principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof .

Claims (7)

1. a grafting method for macadamia nut seed bud, is characterized in that comprising the steps:
Step (1), presprouting of seeds: the seed cultivating stock is taken out from freezer, be placed in shady and cool local stacking 20-30 hour, then bask seeds 2-3 hour in the sun, afterwards seed is dipped in 20-30 hour in water, the seed of removing floating on water surface, goes out tiling to vernalization in constant temperature and humidity room, obtains the seed that season crack width reaches 1.5-2mm by remaining seed flood;
Step (2), cultivates stock: dig kind of a plant hole in nutritious bag matrix center, the season crack width obtained is reached the opening down sowing of seed of 1.5-2mm in step (1), after planting seed, water permeable, then nutritious bag is positioned in plastic tunnel cultivates 20-30 days, obtain the stock of bronze phase;
Step (3), gather scion: clip improved seeds elite stand then raw semi-lignified, healthy branch Zuo is Miho bar, and fringe bar is put into clear water immediately and soaked after cutting, take out drying water after soaking completely, load in moisture-keeping container immediately afterwards, be positioned over shady and cool place and preserve;
Step (4), cut scion: the scion that step (3) is obtained from branch base portion, upwards leaf-cutting successively, every wheel blade retains 1 blade, is then whittled into the short fringe that a fringe one takes turns leaf bud; Bevel must ensure smooth, smooth, clean, obtains fringe bar to be grafted;
Step (5), process scion: the fringe bar to be grafted step (4) obtained is immersed in 9-11 minute in disinfectant, after be immersed in nutrient solution, soak time more than 1 hour, can not obtain grafting scion; Described nutrient solution comprises following component: methyl α-naphthyl acetate 1g, indolebutyric acid 1g, many receipts liquid 30g, 1 liter, water;
Step (6), cut anvil seedling: bronze phase stock step (2) obtained cuts off at distance Nutrition Soil 3cm place, the part of removing more than 3cm, and then from the downward partial application in the centre of otch plane, incision length 2cm, obtains graft stock otch;
Step (7), grafting: the graft stock otch that the grafting scion inserting step (6) step (5) obtained obtains, grafting , Shi Miho bar and stock are combined closely;
Step (8), except rudiment, hardening: 11-13 days grafting mouths after grafting start healing, at this moment need to open greenhouse film transom 1-2 hour every day, the rudiment that stock of erasing in time grows; Miho bar erases unnecessary branch after germinateing, and stays next branch to cultivate as major branch;
Step (9), truncation is done surely: truncation, and after truncation, scion takes out the tip more than 2 times, and the blade of the 2nd tip is aging can go out garden field planting.
2. the grafting method of macadamia nut seed bud according to claim 1, is characterized in that: in step (1), the temperature of vernalization is 33-35 DEG C, and humidity is 85-95%.
3. macadamia nut seed bud grafting method according to claim 1, is characterized in that: the specification of planting plant hole in step (2) is diameter × be 3 × 5cm deeply.
4. the grafting method of macadamia nut seed bud according to claim 1, it is characterized in that: described in step (4) to be whittled into the method that a fringe one takes turns the short fringe of leaf bud be scion be held between left hand thumb and middle finger, one side is close in forefinger tip, at a cutter of splaying apart from 4-5cm place of bastem portion, inclined-plane is at 45 °; Overturn that scion is flat cuts a cutter again, deeply reach xylem, the long 1.5-2cm of bevel, the finally oblique partial application in 1cm place above bud point, cut off scion, to obtain final product.
5. the grafting method of macadamia nut seed bud according to claim 1, is characterized in that: the grafting mouth of the grafting described in step (7) needs to clamp with Grafting clip.
6. the grafting method of macadamia nut seed bud according to claim 1, is characterized in that: the temperature that will keep 25-32 DEG C in step (8) after grafting in booth, the humidity of more than 95%.
7. the grafting method of macadamia nut seed bud according to claim 1, is characterized in that: in step (9), truncation is for cultivating major branch at height of seedling 50cm place truncation.
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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104938181A (en) * 2015-06-12 2015-09-30 岑溪市金特澳洲坚果发展中心 Efficient macadimia nut cultivation method
CN105052613A (en) * 2015-08-20 2015-11-18 中国热带农业科学院南亚热带作物研究所 Macadimia nut seedling raising substrate preparation method based on macadimia nut waste
CN105248154A (en) * 2015-10-30 2016-01-20 云南祥凡农业科技开发股份有限公司 Propagation method of macadamia integrifolia
CN105432329A (en) * 2015-12-01 2016-03-30 广西合山市润鸿农业技术有限公司 Cultivation method for increasing grafting survival rate of macadimia nuts
CN105493993A (en) * 2015-12-01 2016-04-20 广西合山市润鸿农业技术有限公司 Rapid cultivation method of macadamia nut rootstocks
CN106973704A (en) * 2017-03-27 2017-07-25 西双版纳云濮农业科技有限公司 A kind of engrafting method of Queensland nut
CN107494104A (en) * 2017-07-21 2017-12-22 澜沧绿普生农业开发有限公司 A kind of Queensland nut fast breeding method
CN110393119A (en) * 2019-07-31 2019-11-01 广西澳一澳农业科技有限公司 A kind of Queensland nut seed fast seedling growing propagation method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101574043A (en) * 2009-06-15 2009-11-11 云南迪思企业集团坚果有限公司 Bud stock grafting method of Australian nuts
CN102742458A (en) * 2012-07-27 2012-10-24 贵州省亚热带作物研究所 Macadamia nut green branch grafting method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101574043A (en) * 2009-06-15 2009-11-11 云南迪思企业集团坚果有限公司 Bud stock grafting method of Australian nuts
CN102742458A (en) * 2012-07-27 2012-10-24 贵州省亚热带作物研究所 Macadamia nut green branch grafting method

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
田大清等: "澳洲坚果芽砧嫁接育苗技术", 《福建林业科技》 *
黎先进等: "澳洲坚果育苗技术试验研究", 《经济林研究》 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104938181A (en) * 2015-06-12 2015-09-30 岑溪市金特澳洲坚果发展中心 Efficient macadimia nut cultivation method
CN105052613A (en) * 2015-08-20 2015-11-18 中国热带农业科学院南亚热带作物研究所 Macadimia nut seedling raising substrate preparation method based on macadimia nut waste
CN105248154A (en) * 2015-10-30 2016-01-20 云南祥凡农业科技开发股份有限公司 Propagation method of macadamia integrifolia
CN105248154B (en) * 2015-10-30 2017-12-08 云南祥凡农业科技开发股份有限公司 Queensland nut propagation method
CN105432329A (en) * 2015-12-01 2016-03-30 广西合山市润鸿农业技术有限公司 Cultivation method for increasing grafting survival rate of macadimia nuts
CN105493993A (en) * 2015-12-01 2016-04-20 广西合山市润鸿农业技术有限公司 Rapid cultivation method of macadamia nut rootstocks
CN106973704A (en) * 2017-03-27 2017-07-25 西双版纳云濮农业科技有限公司 A kind of engrafting method of Queensland nut
CN107494104A (en) * 2017-07-21 2017-12-22 澜沧绿普生农业开发有限公司 A kind of Queensland nut fast breeding method
CN110393119A (en) * 2019-07-31 2019-11-01 广西澳一澳农业科技有限公司 A kind of Queensland nut seed fast seedling growing propagation method

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