CN103749126B - The sustainable cultivation method of a kind of root of three-nerved spicebush - Google Patents
The sustainable cultivation method of a kind of root of three-nerved spicebush Download PDFInfo
- Publication number
- CN103749126B CN103749126B CN201410009909.3A CN201410009909A CN103749126B CN 103749126 B CN103749126 B CN 103749126B CN 201410009909 A CN201410009909 A CN 201410009909A CN 103749126 B CN103749126 B CN 103749126B
- Authority
- CN
- China
- Prior art keywords
- root
- nerved spicebush
- strain
- cultivation method
- soil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The present invention relates to the sustainable cultivation method of a kind of root of three-nerved spicebush.Also there is no the sustainable cultivation method of a kind of root of three-nerved spicebush at present.Feature of the present invention is: comprise the steps: the root of three-nerved spicebush strain that (1) selects life in 3 years above spring or autumn, to cut off the branch of acrial part 2/3rds; (2) excavate the soil of root, whole strain is mentioned, clip root expand block root; (3) by all root prunings to length be the 8-12 of leading thread doubly; (4) wound after being pruned by root is immersed in 5 seconds in the calcium hydroxide aqueous solution of 1%, and dry after taking out, during immersion, the liquid level of calcium hydroxide aqueous solution covers wound, and the distance between liquid level and wound is less than 5mm; (5) soil excavated is admixed the root-inducing powder of 0.1% and the Lachnum zymotic fluid of 1%; (6) root of three-nerved spicebush strain is planted again in the cave that back cutting opens, and mulching soil; (7) water in time; Within (8) three years, repeat block root to gather later.The present invention can sustainable utilization, belongs to environmentally friendly.
Description
Technical field
The present invention relates to a kind of cultivation method, especially relate to the sustainable cultivation method of a kind of root of three-nerved spicebush, root of three-nerved spicebush block root can be obtained for a long time continuously.
Background technology
The root of three-nerved spicebush (
lindera aggregata(Sims) Kosterm.) be Lauraceae, Lindera plant, be born in height above sea level 200-1000 rice and face south in hillside fields, mountain valley or sparse woods shrubbery.Produce the provinces and regions such as Zhejiang, Jiangxi, Fujian, Anhui, Hunan, Sichuan, Guangdong, Guangxi, Shaanxi, Taiwan, be adapted at sunny, the soil is porous fertile acid ground grows.All on the books in the ethnopharmacology classical works such as Compendium of Material Medica, " Kaibao Bencao ", " Chinese medicine voluminous dictionary ", " Bencao Jingshu " and " book on Chinese herbal medicine meets source ", its have loose cold, pain relieving, bowl spares, pleasant, open strongly fragrant, increase enterogastric peristalsis, excited cerebral cortex, accelerate blood circulation, hemostasis, the effect such as antibacterial.
The root of three-nerved spicebush is excavated based on wild at present, because the root of three-nerved spicebush is used as medicine with block root, its taproot can not hyoscine, and the field root of three-nerved spicebush of gathering is all for cost substantially with the death of whole strain plant, root of three-nerved spicebush strain abandons after block root is gathered in excavation, can cause the problem such as environmental disruption and water and soil loss again.
Also there is no the sustainable cultivation method of a kind of root of three-nerved spicebush at present, if publication date is on 08 28th, 2013, publication number is in the Chinese patent of CN103262736A, discloses a kind of Radix Codonopsis cultivation method of efficient volume increase, and this cultivation method comprises the steps: that the front soil of training seedling prepares, seed preparation; Sowing; Rapid seedling cultivation a, employing first generation plantlet in vitro, as propagating materials; B, when seedbed seedling grows to 25 centimetres high, stem apex, stem section are formed, just can start to get its stem apex, stem section, wound is immersed 500ppm rooting powder solution and is disposed, direct inoculation agricultural facility booth hotbed, stem apex stem section starts to survive regeneration in hotbed soil, temperature 28 ~ 35 DEG C growth 30 days, forms second generation whole plant; After c, second generation whole plant are formed, continue to get its plants stems section and stem apex continues inoculation reproduction in agricultural facility booth seedbed, after 30 days, third generation plant seedling is formed, 30 days cycles can emerge for kind; Autumn sowing is advisable to transplant autumn, and adopt ridge to plant or ridge cultivation, this cultivation method is not suitable for the cultivation root of three-nerved spicebush.
Summary of the invention
The object of the invention is to overcome traditional root of three-nerved spicebush to gather the deficiency such as the wasting of resources and environmental disruption of middle existence, and provide a kind of can sustainable utilization, the sustainable cultivation method of the environmentally friendly root of three-nerved spicebush.
The present invention's adopted technical scheme that solves the problem is: the feature of the sustainable cultivation method of this root of three-nerved spicebush is: comprise the steps:
(1) select the wild or cultivation root of three-nerved spicebush strain that life in 3 years is above, spring or autumn, cut off the branch of acrial part 2/3rds, obtain the beta pruning root of three-nerved spicebush; This step is tried one's best the longer root of three-nerved spicebush strain of growth selection time, at least take life in 3 years, according to the research of inventor, the root of three-nerved spicebush that preferably life in 5 years is above, pruned away by the acrial part of 2/3rds, after preventing from again planting back, root of three-nerved spicebush transpiration is excessive, dehydration is dead, also facilitates gathering and heavily planting of next step.
(2) excavate the soil of beta pruning root of three-nerved spicebush root, whole for beta pruning root of three-nerved spicebush strain is mentioned, expand block root with scissors clip beta pruning root of three-nerved spicebush root; This step is the root of three-nerved spicebush block root of gathering up to standard, notes carefully excavating root of three-nerved spicebush root soil, and the block root that do not make to expand is retained in soil.
(3) by all root prunings of the beta pruning root of three-nerved spicebush to length be the 8-12 of leading thread doubly; This step one is to cultivate conveniently, long so as not to root of three-nerved spicebush root, bendingly in cave is unfavorable for growth, and two is all cut short all, is conducive to growing and the formation of block root of the new root of the root of three-nerved spicebush.
(4) it was 5 seconds in the calcium hydroxide aqueous solution of 1% that the wound after being pruned by beta pruning root of three-nerved spicebush root is immersed in mass percent, dried after taking out, and when wound soaks, the liquid level of calcium hydroxide aqueous solution covers wound, and the distance between liquid level and wound is less than 5mm; Adopt the calcium hydroxide aqueous solution (limewash) of low concentration to process root of three-nerved spicebush root wound, prevent saprophytic bacteria from infecting, method is simple, and raw material is easy to get, and process is convenient.Calcium hydroxide aqueous solution is as far as possible few to be used, because according to the research of inventor, have in the root of three-nerved spicebush growing environment of block root, soil pH value is all in 4.6 ± 1.0 scopes, so low-concentration hydrogen calcium oxide aqueous solution does not cover more than wound 5mm, both played sterilization functions, the acid-base value of soil can not be had influence on again.
(5) soil excavated in step (2) is admixed mass percent be 0.1% root-inducing powder and mass percent be 1% Lachnum zymotic fluid; Root-inducing powder plays rooting efficiency to the root of three-nerved spicebush, Lachnum is the endogenetic fungus of root of three-nerved spicebush block root, closely related with the generation of secondary metabolites in block root, and the growth of root of three-nerved spicebush side root can be stimulated, be conducive to the formation of root of three-nerved spicebush block root, in addition soil is processed, play the effect of loosening the soil, be conducive to the growth of root of three-nerved spicebush root and the growth of plant.
(6) root of three-nerved spicebush strain handled well in step (4) is planted again in the cave that back cutting opens, and cover the soil that step (5) processed; The root of three-nerved spicebush of beta pruning, block root of gathering is planted back in original cave again, ridging, covering.
(7) root of three-nerved spicebush strain that Xiang Chongzhi is good is watered in time; The root of three-nerved spicebush of again planting back after cutting root, except watering normal root water in time, also needs often to water; Gather spring the root of three-nerved spicebush of replanting, shelter from heat or light to autumn with the shade net scaffolding of 50% light transmission capacity, Second Year is without the need to sheltering from heat or light, the root of three-nerved spicebush of replanting gathering autumn without the need to sheltering from heat or light, growing the wild root of three-nerved spicebush in forest, or the bionic cultivation root of three-nerved spicebush in mixed forest, forest canopy density more than 0.5, also without the need to sheltering from heat or light.Gather the root of three-nerved spicebush, the particularly wild root of three-nerved spicebush after replanting and the bionic cultivation root of three-nerved spicebush, First Year will water in time, and Second Year is later then without the need to management.
(8) root of three-nerved spicebush strain of again cultivating, repeats block root in 3 years later and gathers.Gather the root of three-nerved spicebush strain of replanting, the block root again born for the 3rd year can be gathered, but amount of growth is less, and the suitableeest is gather once for 5 years.
Make root of three-nerved spicebush strain in the present invention can sustainable utilization thus, shorten the root of three-nerved spicebush block root growth cycle, be conducive to protection of the environment.
As preferably, the root of three-nerved spicebush strain in step of the present invention (1) is the above root of three-nerved spicebush of life in 5 years.
As preferably, in step of the present invention (3), be 10 times of leading thread by all root prunings of the beta pruning root of three-nerved spicebush to length.
As preferably, the root-inducing powder in step of the present invention (5) is ABT1 root-inducing powder.ABT1 root-inducing powder is the most obvious to root of three-nerved spicebush rooting efficiency,
As preferably, in step of the present invention (7), the shade net of the root of three-nerved spicebush strain light transmission capacity 50% of heavily having planted scaffolding is sheltered from heat or light.
As preferably, in step of the present invention (8), every 5 years Repeated Harvesting block roots once.
The present invention compared with prior art, has the following advantages and effect: (1) root of three-nerved spicebush plant can reuse, and a root of three-nerved spicebush block root of gathering for more than 3 years, shortens the root of three-nerved spicebush block root growth cycle; (2) avoid traditional root of three-nerved spicebush to gather the shortcoming of pattern, comprise root of three-nerved spicebush block root gather after whole strain abandon the wasting of resources caused, and the environmental disruption problem such as the water and soil loss caused after root of three-nerved spicebush death; (3) wound after utilizing calcium hydroxide aqueous solution to shear block root carries out immersion treatment, prevents root-rot bacterium from destroying the dip-dye of wound; (4) root-inducing powder and root of three-nerved spicebush endogenetic fungus Lachnum zymotic fluid is utilized to process soil, root-inducing powder and the acting in conjunction of Lachnum zymotic fluid, effectively can promote the growth of root of three-nerved spicebush block root, stimulate the secretion of secondary metabolite in root of three-nerved spicebush block root, root of three-nerved spicebush beet yield and quality can be improved.
Embodiment
Below by embodiment, the present invention is described in further detail, and following examples are explanation of the invention and the present invention is not limited to following examples.
Embodiment 1.
The sustainable cultivation method of the root of three-nerved spicebush in the present embodiment comprises the steps.
(1) select the triennial wild root of three-nerved spicebush, leading thread is about 0.5cm, cuts off the branch of acrial part 2/3 April in spring.
(2) excavate the soil of beta pruning root of three-nerved spicebush root, whole strain is mentioned, with scissors by root swelling block root clip, and the block root that clip gets off about 3.7 grams.
(3) be 5cm by all for root of three-nerved spicebush root prunings to length.
(4) root being pruned wound, to be immersed in mass percent be 5 seconds in 1% calcium hydroxide aqueous solution, dries after taking out, and when noticing that wound soaks, calcium hydroxide aqueous solution does not cover more than wound 5mm.
(5) soil excavated is admixed mass percent be 0.1% ABT1 root-inducing powder and mass percent be 1% Lachnum zymotic fluid.
(6) root of three-nerved spicebush strain that step (4) is handled well is planted again in the cave that back cutting opens, and cover the soil that upper step (5) processed.
(7) root of three-nerved spicebush strain of will heavily plant, shelter from heat or light with the shade net of light transmission capacity 50% scaffolding, watered the same day and irrigate, within the 7th day, water once, water thereafter water spacer increases by two days at every turn, has to rain that then the time postpones, to October then without the need to rewatering.
Carry out above-mentioned piece of root after (8) three years again to gather, the block root of gathering about 1.5 grams.
The sustainable cultivation method of the root of three-nerved spicebush in the present embodiment repeats above-mentioned steps (1) to step (8), and belonging to can sustainable utilization, environmentally friendly cultivation method.
Embodiment 2:
The sustainable cultivation method of the root of three-nerved spicebush in the present embodiment comprises the steps.
(1) in the wealthy mixed forest of the pine of crown density 0.6, select the bionic cultivation root of three-nerved spicebush 100 strain of life in 6 years, leading thread is about 1.0cm, cuts off the branch of acrial part 2/3 April in spring.
(2) excavate the soil of beta pruning root of three-nerved spicebush root, whole strain is mentioned, with scissors by root swelling block root clip, and the block root that every strain clip gets off about 31.1 ± 3.7 grams.
(3) be 10cm by all for root of three-nerved spicebush root prunings to length.
(4) root being pruned wound, to be immersed in mass percent be 5 seconds in 1% calcium hydroxide aqueous solution, dries after taking out, and when noticing that wound soaks, calcium hydroxide aqueous solution does not cover more than wound 5mm.
(5) soil excavated is admixed mass percent be 0.1% ABT1 root-inducing powder and mass percent be 1% Lachnum zymotic fluid.
(6) root of three-nerved spicebush strain that step (4) is handled well is planted again in the cave that back cutting opens, and cover the soil that upper step (5) processed.
(7) root of three-nerved spicebush strain of heavily having planted, without the need to process of sheltering from heat or light, watered and irrigated the same day, and within the 7th day, water once, water thereafter water spacer increases by two days at every turn, have rain then the time postpones, to October then without the need to rewatering.
Select 50 strains to carry out above-mentioned piece of root after (8) three years to gather, the block root about 12.7 ± 3.8 grams that every strain is gathered; After 5 years, above-mentioned piece of root is carried out in other 50 strains again to gather, the block root about 24.0 ± 6.6 grams that every strain is gathered.
The sustainable cultivation method of the root of three-nerved spicebush in the present embodiment repeats above-mentioned steps (1) to step (8), and belonging to can sustainable utilization, environmentally friendly cultivation method.
Embodiment 3.
The sustainable cultivation method of the root of three-nerved spicebush in the present embodiment comprises the steps.
(1) select the wild or cultivation root of three-nerved spicebush strain that life in 3 years is above, spring or autumn, cut off the branch of acrial part 2/3rds, obtain the beta pruning root of three-nerved spicebush.
(2) excavate the soil of beta pruning root of three-nerved spicebush root, whole for beta pruning root of three-nerved spicebush strain is mentioned, expand block root with scissors clip beta pruning root of three-nerved spicebush root.
(3) by all root prunings of the beta pruning root of three-nerved spicebush to length be the 8-12 of leading thread doubly.
(4) it was 5 seconds in the calcium hydroxide aqueous solution of 1% that the wound after being pruned by beta pruning root of three-nerved spicebush root is immersed in mass percent, dried after taking out, and when wound soaks, the liquid level of calcium hydroxide aqueous solution covers wound, and the distance between liquid level and wound is less than 5mm.
(5) soil excavated in step (2) is admixed mass percent be 0.1% root-inducing powder and mass percent be 1% Lachnum zymotic fluid, namely root-inducing powder is 0.1% of soil quality, and Lachnum zymotic fluid is 1% of soil quality.
(6) root of three-nerved spicebush strain handled well in step (4) is planted again in the cave that back cutting opens, and cover the soil that step (5) processed.
(7) root of three-nerved spicebush strain that Xiang Chongzhi is good is watered in time.
(8) root of three-nerved spicebush strain of again cultivating, repeats block root in 3 years later and gathers.
The sustainable cultivation method of the root of three-nerved spicebush in the present embodiment repeats above-mentioned steps (1) to step (8), and belonging to can sustainable utilization, environmentally friendly cultivation method.
Embodiment 4.
The sustainable cultivation method of the root of three-nerved spicebush in the present embodiment comprises the steps.
(1) select the wild or cultivation root of three-nerved spicebush strain that life in 5 years is above, spring or autumn, cut off the branch of acrial part 2/3rds, obtain the beta pruning root of three-nerved spicebush.Root of three-nerved spicebush strain in the present invention also can be the above root of three-nerved spicebush of life in 3 years.
(2) excavate the soil of beta pruning root of three-nerved spicebush root, whole for beta pruning root of three-nerved spicebush strain is mentioned, expand block root with scissors clip beta pruning root of three-nerved spicebush root.
(3) by all root prunings of the beta pruning root of three-nerved spicebush to length be the 8-12 of leading thread doubly.Be generally 10 times of leading thread by all root prunings of the beta pruning root of three-nerved spicebush to length.
(4) it was 5 seconds in the calcium hydroxide aqueous solution of 1% that the wound after being pruned by beta pruning root of three-nerved spicebush root is immersed in mass percent, dried after taking out, and when wound soaks, the distance between the liquid level of calcium hydroxide aqueous solution and wound is less than 5mm.
(5) soil excavated in step (2) is admixed mass percent be 0.1% root-inducing powder and mass percent be 1% Lachnum zymotic fluid.Root-inducing powder is preferably ABT1 root-inducing powder.
(6) root of three-nerved spicebush strain handled well in step (4) is planted again in the cave that back cutting opens, and cover the soil that step (5) processed.
(7) shade net of the root of three-nerved spicebush strain light transmission capacity 50% of heavily having planted scaffolding is sheltered from heat or light, and water in time.
(8) root of three-nerved spicebush strain of again cultivating, repeats block root in 3 years later and gathers, and is preferably every 5 years Repeated Harvesting block roots once.
The sustainable cultivation method of the root of three-nerved spicebush in the present embodiment repeats above-mentioned steps (1) to step (8), and belonging to can sustainable utilization, environmentally friendly cultivation method.
Mode below by experiment is next, and the present invention is described further, and result of study is as follows.
A, root of three-nerved spicebush branch cutting are tested, and somatotropin contrasts.
Gather 3 years raw root of three-nerved spicebush seedling branches, be cut into 10 centimeter length, lower cut is horse ear shape, sheared-off part blade, soaks in treatment fluid, is divided into No. 1, ABT root-inducing powder, No. 2, ABT root-inducing powder, naa (NAA), indolebutyric acid (IBA) and contrast (CK) 5 process, concentration is 100PPM, soak base portion 12h, soaking depth 2cm, then cuttage.
Result shows, No. 1, ABT root-inducing powder is best to root of three-nerved spicebush cuttage branch rooting efficiency.
B, endogenetic fungus are tested.
Inventor utilizes sterile water and ethanol, liquor natrii hypochloritis successively to the sterilizing of root of three-nerved spicebush block root, cultivates, purifying, Zengjing Granule, obtain the mycelium of root of three-nerved spicebush endogenetic fungus, extract its DNA, increase through rDNA-ITS, order-checking, qualification, identifying root of three-nerved spicebush endogenetic fungus is Lachnum.The Lachnum shaking table of acquisition being cultivated, is mixed with the strong microbial inoculum mud of 1%, when transplanting not dip in cuttage seeding that microbial inoculum directly transplants for contrast (CK), comparing.
Result shows, extremely significantly (P<0.01), with the process of microbial inoculum, side radical is obviously more than the process not with microbial inoculum for the result difference of two kinds of processing methods, and root of three-nerved spicebush block root primary growth is in side root, so microbial inoculum process can effectively increase transplant survival rate and block root growth rate.
C, following table are planted back in original cave for inventor gathers after block root to six groups of all ages and classes stage roots of three-nerved spicebush again, secondary recovery block root after 3 years, the contrast of twice amount of gathering.
Although the present invention with embodiment openly as above; but it is also not used to limit protection scope of the present invention; any technical staff being familiar with this technology, not departing from the change and retouching done in the spirit and scope of the present invention, all should belong to protection scope of the present invention.
Claims (6)
1. the sustainable cultivation method of the root of three-nerved spicebush, is characterized in that: comprise the steps:
(1) select the wild or cultivation root of three-nerved spicebush strain that life in 3 years is above, spring or autumn, cut off the branch of acrial part 2/3rds, obtain the beta pruning root of three-nerved spicebush;
(2) excavate the soil of beta pruning root of three-nerved spicebush root, whole for beta pruning root of three-nerved spicebush strain is mentioned, expand block root with scissors clip beta pruning root of three-nerved spicebush root;
(3) by all root prunings of the beta pruning root of three-nerved spicebush to length be the 8-12 of leading thread doubly;
(4) it was 5 seconds in the calcium hydroxide aqueous solution of 1% that the wound after being pruned by beta pruning root of three-nerved spicebush root is immersed in mass percent, dried after taking out, and when wound soaks, the liquid level of calcium hydroxide aqueous solution covers wound, and the distance between liquid level and wound is less than 5mm;
(5) soil excavated in step (2) is admixed root-inducing powder and Lachnum zymotic fluid, root-inducing powder is 0.1% of soil quality, and Lachnum zymotic fluid is 1% of soil quality;
(6) root of three-nerved spicebush strain handled well in step (4) is planted again in the cave that back cutting opens, and cover the soil that step (5) processed;
(7) root of three-nerved spicebush strain that Xiang Chongzhi is good is watered in time;
(8) root of three-nerved spicebush strain of again cultivating, repeats block root in 3 years later and gathers.
2. the sustainable cultivation method of the root of three-nerved spicebush according to claim 1, is characterized in that: the root of three-nerved spicebush strain in described step (1) is the above root of three-nerved spicebush of life in 5 years.
3. the sustainable cultivation method of the root of three-nerved spicebush according to claim 1, is characterized in that: in described step (3), is 10 times of leading thread by all root prunings of the beta pruning root of three-nerved spicebush to length.
4. the sustainable cultivation method of the root of three-nerved spicebush according to claim 1, is characterized in that: the root-inducing powder in described step (5) is ABT1 root-inducing powder.
5. the sustainable cultivation method of the root of three-nerved spicebush according to claim 1, is characterized in that: in described step (7), is sheltered from heat or light by the shade net of the root of three-nerved spicebush strain light transmission capacity 50% of heavily having planted scaffolding.
6. the sustainable cultivation method of the root of three-nerved spicebush according to claim 1, is characterized in that: in described step (8), and every 5 years Repeated Harvesting block roots once.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410009909.3A CN103749126B (en) | 2014-01-09 | 2014-01-09 | The sustainable cultivation method of a kind of root of three-nerved spicebush |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410009909.3A CN103749126B (en) | 2014-01-09 | 2014-01-09 | The sustainable cultivation method of a kind of root of three-nerved spicebush |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103749126A CN103749126A (en) | 2014-04-30 |
| CN103749126B true CN103749126B (en) | 2015-10-28 |
Family
ID=50516586
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410009909.3A Active CN103749126B (en) | 2014-01-09 | 2014-01-09 | The sustainable cultivation method of a kind of root of three-nerved spicebush |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103749126B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106358683A (en) * | 2016-08-29 | 2017-02-01 | 青川县蜀道地中药材发展有限公司 | Standard culturing method of Qingchuan lindera aggregata |
| CN106912349B (en) * | 2017-03-01 | 2020-03-13 | 济南大学 | Method for promoting accumulation of luteoloside in honeysuckle flower buds by using fungi |
| CN112535080B (en) * | 2020-12-10 | 2022-04-08 | 浙大宁波理工学院 | Method for improving formation rate of fusiform tuberous roots for combined spicebush root medicines |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487687A (en) * | 2011-12-01 | 2012-06-13 | 广州康和药业有限公司 | Method for planting flaccid anemone rhizome in industrialized form and application thereof |
-
2014
- 2014-01-09 CN CN201410009909.3A patent/CN103749126B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487687A (en) * | 2011-12-01 | 2012-06-13 | 广州康和药业有限公司 | Method for planting flaccid anemone rhizome in industrialized form and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| "天台乌药内生菌的分离培养与分子鉴定";王衍斌等;《第十届全国药用植物及植物药学术研讨会论文摘要集》;20110812;第138页 * |
| "天台乌药嫩枝扦插育苗技术研究";秦玉川等;《江西林业科技》;20100831;第13-14页 * |
| 郭生桢主编.五、采收、加工与贮藏.《药用植物栽培技术》.陕西科学技术出版社,1987,第290-291页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103749126A (en) | 2014-04-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110352804A (en) | A kind of acclimatization and transplants method of savatier monochasma herb | |
| CN102113445B (en) | Method for raising seedlings of Pinus kesiya var.langbianensis by efficient twig cuttage propagation | |
| CN104737828B (en) | Chinese chestnut branch embedding grafting method | |
| CN101292601A (en) | High quality high production cultivation method for kudzu root | |
| CN104585007A (en) | Cuttage seedling culture method of liquidambar styraciflua | |
| CN105145098A (en) | Cutting propagation method for brucea javanica | |
| CN102067781B (en) | Regeneration and cultivation technology for cortex magnoliae officinalis | |
| CN109089687A (en) | Borneol camphor tree cuttage and seedling culture propagation method | |
| CN105961125A (en) | Method for planting cape jasmine | |
| CN103416209A (en) | Artificial cultivation method of wild sweet tea tree | |
| CN105325149A (en) | Cuttage planting method of lignified arrowroots | |
| CN103749126B (en) | The sustainable cultivation method of a kind of root of three-nerved spicebush | |
| CN106856922A (en) | Chinese lobelia cuttage breeding method | |
| CN104904537B (en) | Plum tree woodland seedling culturing method | |
| CN105230433A (en) | Cutting propagation method for American red maples | |
| CN104855229A (en) | Pachira macrocarpa planting method | |
| CN104904555A (en) | Bonsai manufacturing method of seedling red maple | |
| CN104012282A (en) | Method for growing vatica astrotricha seedlings in Guangxi | |
| CN104206186B (en) | A kind of engrafting method of Sassafras tsumu Hemsl | |
| CN101554115B (en) | Method for culturing Chinese silvergrass with high yield | |
| CN103749129B (en) | Method for asexually and rapidly propagating homalium hainanense | |
| CN108770623A (en) | The overwintering no suspend mode hardening off method of bletilla tissue culture seedlings duplicature | |
| CN103782789A (en) | Cultivation method for sonneratia apetala Buch-Ham seedlings | |
| CN103141287A (en) | Method for domesticating and culturing rubus peltatus | |
| CN106718553A (en) | A kind of method for planting of orange tree potted landscape |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |