CN105265184A - Cotton grafting splice grafting method - Google Patents

Abstract

A cotton grafting splice grafting method comprises the following steps: 1, splicing a stock into a smooth slope, vertically cutting the stock to the bottom of the slope along a stem middle axle, the cutting is in a V-shape, and the stock reserves one or more function blades; 2, cutting scion into a slope with about same size of the stock, vertically cutting the scion to the bottom of the slope along an axle, the cutting is in an inverted V-shape, and one or more blades are kept; 3, closely jointing two inner sections with two outer sections of the scion and the stock in a staggering manner until the bottoms of the sections are reached, i.e., the two Vs are buckled, if the stock and the scion are different in stem diameter, the stem side in the joint should be smooth, and phloem should be fully fit; 4, using a grafting clamp to fasten the joint between the stock and the scion; 5, keeping humidity >=80% and temperature 25-35 DEG C after grafting, shading the top; 6, removing the shading after the grafted cotton passes a recovery period and the scion blade becomes flat, gradually airing for dehumidification, and completing the grafting.

Description

A kind of cotton grafting and fit grafting method

Technical field

The present invention relates to a grafting method-cotton grafting and fit grafting method grown cotton.

Background technology

Along with cotton cells engineering and engineered development, the dissimilar test-tube plantlet such as the Regenerated Plants From Somatic Cells of acquisition, transfer-gen plant and IMMATURE EMBRYOS CULTURE plant gets more and more, but its transplant survival is very difficult.The methods such as the direct field planting of usual employing, vermiculite (sand) or nutrient solution transition cultivation are transplanted, and cotton plant transplanting seedling time is long, and survival rate is very low, governs the application of biotechnology on cotton genetic improvement to a certain extent.Therefore, the test-tube plantlet planting survival rates improving transgenic cotton plant and other type becomes problem demanding prompt solution.In recent years, some reports adopt grafting method to solve test-tube plantlet field planting problem and achieve certain effect.Grafting method over-borrowing conventional " wedge joint method ", " tip cutting connection " of fruit tree and vegetable or its improvement method etc., its survival rate is 60%-80%.The present invention aims to provide a kind of grafting method being suitable for easy, the high-servival rate of cotton, and this invention graft survival rate Absorbable organic halogens, more than 95%, coordinates phase graft survival rate to reach 100% at suitable stock and scion.

Summary of the invention

The present invention is achieved through the following technical solutions: the healthy and strong cotton seedling of (1) pre-incubation does stock, the seedling age of the seedling age >=scion of stock; (2) with sharp thin blade, stock growing point and unnecessary cauline leaf are excised, retain >=1 functional leaf; (3) the stem top of reservation is whittled into level and smooth inclined-plane from top to bottom, the about long 0.5cm-1.5cm in inclined-plane (when seedling age is larger, inclined-plane can be grown a little again); (4) from inclined-plane about 1/3 place bottom stem axis cutting perpendicularly to inclined-plane, otch at the cross section middle part of stem, length >=0.3cm, otch is in " V " font; (5) scion retains 1-2 sheet leaf, and the root of bottom and unnecessary cauline leaf are removed, scion is whittled into the inclined-plane suitable with stock from bottom to top, and vertically rives to inclined-plane along axis, long incision >=0.3cm, and otch is in falling " V " font; (6) by scion and stock 2 interior tangent planes, 2 outer tangent planes staggered, intimate engagement between two, be inserted to bottom tangent plane, i.e. stock and scion " V " word is staggered interlocks.If stock is different with the stem thickness of scion, the side of joint stem should be made level and smooth, and phloem is completely closely sealed; (7) step up fixing stock and scion joint with the wide mouth Grafting clip that degree of tightness is suitable, Grafting clip can not crush cortex and have abundant fixed width; (8) water after grafting, on cover, plastic film is obturaged, and keeps humidity >=80%.Shading in top, avoids direct sunlight, temperature 25 DEG C-35 DEG C; (9) 1-5 days after grafting, scion leaf recovers open and flat when tiding over seedling-slowing stage, removes the thing that shades, and moisture-inhibiting of leaking informaton gradually, finally remove plastic film, complete grafting.

This invention grafting cotton, need descend cutter accurate, and otch is agilely level and smooth, keeps humidity >=80% after grafting, graft survival rate can >=95%.When scion is the cotton seedling that cotyledon is deployed into 1 true leaf size, when stock is the cotton seedling of 1-3 sheet true leaf, graft survival rate can reach 100%, and this phase is the best seedling age phase that stock and scion coordinate.In addition, stock seedling age is greater than scion ages and differs 1-2 sheet true leaf is good.

This invention not only can be applicable to the grafting of the multiple test-tube plantlets such as the training of transgenosis group, IMMATURE EMBRYOS CULTURE and conventional cotton plant stem apex, fruit branch, leaf branch, also can be used for the grafting of other crops such as soybean.

Embodiment

Can know the present invention further by specific embodiment given below, but they not limitations of the present invention.

Embodiment 1: cotton 20 cotton seedling 6 basins in Growth of Potted Cotton cultivar Ji, the strain of every basin 5, totally 30 strains, as stock.The cotton 20 other seeds in sterilization Ji after two weeks, on medium, the cotton seedling of aseptic culture is used as scion, and when tassel leaf waiting launches, greenhouse stock is grown to the cotton seedling of 2 true leaves.Cut with upper part by stock true leaf, retain 2cm-3cm stem, stem is whittled into about 1.5cm inclined-plane, from inclined-plane about 1/3 place along bottom stem axis cutting perpendicularly to stem cross section, the about long 1.0cm of otch, in " V " font.Scion goes root to retain the long stem of 2cm-3cm, is whittled into onesize inclined-plane, vertically rives along axis, and otch is about 1.0cm in falling " V " font.Carefully by scion and stock 2 interior tangent planes, 2 outer tangent planes closely staggered joint between two, be inserted to bottom tangent plane, stock and scion " V " word interlock and interlock.Fixing stock and scion joint is stepped up with the flat mouth Grafting clip that about 1.0cm is wide.Water after grafting, prop small wooden around grafting plant, plastic film on cover also seals, and film top newspaper shades.Water every day once, keep relative moisture 85%-100%, after 2 days, grafting plant tides over seedling-slowing stage, and blade launches again.Removing newspaper, moisture-inhibiting of leaking informaton gradually, within after grafting 4 days, remove plastic film completely, grafting cotton plant all survives, survival rate 100%.

Claims (2)

1. a cotton grafting and fit grafting method, is characterized in that, comprises following sequential steps: (1) stock is whittled into smooth ramp and bottom stem axis cutting perpendicularly to inclined-plane, otch becomes " V " font, and stock retains >=1 functional leaf; (2) scion is whittled into the sizable inclined-plane with stock, vertically rives to inclined-plane along axis, and otch, in falling " V " font, retains >=1 blade; (3) by scion and stock 2 interior tangent planes, 2 outer tangent planes closely staggered joint between two, be inserted to bottom tangent plane, namely two " V " word interlocks, if stock is different with the stem thickness of scion, the side of joint stem should be made level and smooth, and phloem is completely closely sealed; (4) Grafting clip steps up fixing stock and scion joint; (5) keeping humidity >=80% after grafting, temperature 25 DEG C-35 DEG C, shades in top; (6) grafting cotton tides over seedling-slowing stage, when scion leaf recovers open and flat, removes the thing that shades, leaks informaton gradually and dry, complete grafting.

2. cotton grafting and fit grafting method according to claim 1, it is characterized in that 2 interior tangent planes of described scion and stock and 2 outer tangent planes between two staggered relative insert bottom tangent planes, intimate engagement, i.e. stock and scion " V " word is staggered interlocks, insertion depth >=0.3cm, Grafting clip is clipped in bonding station.