CN101194570A - Sprout grafting method for fructus schizandrae - Google Patents
Sprout grafting method for fructus schizandrae Download PDFInfo
- Publication number
- CN101194570A CN101194570A CNA2006101632095A CN200610163209A CN101194570A CN 101194570 A CN101194570 A CN 101194570A CN A2006101632095 A CNA2006101632095 A CN A2006101632095A CN 200610163209 A CN200610163209 A CN 200610163209A CN 101194570 A CN101194570 A CN 101194570A
- Authority
- CN
- China
- Prior art keywords
- grafting
- wils
- stock
- schisandra sphenanthera
- sphenanthera rehd
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention relates to a bud seedling grafting method for schisandra fruit, which belongs to the breeding technique in the crop cultivation science field. The grafting method for schisandra fruit takes seeds of schisandra fruit after germinating whose cotyledons stretch before true leaves stretch as a rootstock, and takes green branches of schisandra fruit as grafts and graft to grow a new plant. The grafts are semi-lignified new shoots or lateral shoots or young shoots which are tissue-cultivated. The grafting is that grafts are connected in a slot on a lower hypocotyl of the rootstock, which guarantees that grated breed seedlings can be cultivated on the year that a rootstock is sowed, reduces grafting cost, shortens age limit of seedling breed and improves grafting efficiency evidently, and the grafting survival rate reaches about 95%. The invention can be applied to grafting seedling production of schisandra fruit, and can promote fructifying in advance, and avoids generation of underground traverse stems.
Description
Technical field:
The present invention relates to the sprout grafting method of a kind of Schisandra sphenanthera Rehd.et WilS, belong to the propagation technique in the crop cultivation field.
Background technology:
Schisandra sphenanthera Rehd.et WilS is the large genuine traditional Chinese medicine material of main product in China northeast, because that the increase of the market demand and wild resource destroy is serious day by day, the artificial cultivation development of Schisandra sphenanthera Rehd.et WilS rapidly in recent years.Because the imperfection of Schisandra sphenanthera Rehd.et WilS vegetative propagation technique, the artificial cultivation of current Schisandra sphenanthera Rehd.et WilS still mainly adopts seedling to build the garden, variation between plant is big, and quality is irregular, the maturing stage does not concentrate, problems such as resistance is uneven, high and stable yield difference are serious, has greatly limited the development of this industry.Therefore, the efficient vegetative propagation technique of research and development Schisandra sphenanthera Rehd.et WilS, and carry out the kind cultivation of Schisandra sphenanthera Rehd.et WilS, significant to the development of schisandra cultivating industry.At present, there is the Schisandra sphenanthera Rehd.et WilS vegetative propagation technique of report mainly to comprise four kinds.(1) stiff wood grafting is a stock with the annual or biennial seedling of Schisandra sphenanthera Rehd.et WilS, is scion with the annual ripe branch of Schisandra sphenanthera Rehd.et WilS, adopts the mode of cleft grafting to carry out grafting.(2) tissue culture is an explant with the branch section of the tender young sprout of Schisandra sphenanthera Rehd.et WilS children, by the inducing culture of bud, mitogenetic cultivation, culture of rootage and acclimatization and transplants supervisor, and the plant that breeding makes new advances.(3) cottage propagation is cutting with green branch or the annual ripe branch of Schisandra sphenanthera Rehd.et WilS, through cuttage on the certain culture base after the chemicals treatment, impels and takes root and form new plant.(4) root turion seedling breeding separates the sprout tillers of Schisandra sphenanthera Rehd.et WilS creeping underground stem and generation thereof with parent, transplant the back and form new plant.
Summary of the invention
The object of the present invention is to provide a kind of Schisandra sphenanthera Rehd.et WilS sprout grafting method, is stock with Schisandra sphenanthera Rehd.et WilS bud seedling, is the grafting method of scion with the green branch of Schisandra sphenanthera Rehd.et WilS; Guarantee the product seedling of just turning out grafting then of stock sowing, reduced the grafting cost, shortened the time limit of seedling-wood breeding; Graft survival rate reaches about 95%, and grafting efficiency significantly improves, and has promoted result ahead of time, and has avoided the generation of creeping underground stem, but large-scale application is produced in the grafting of Schisandra sphenanthera Rehd.et WilS.
Technical scheme of the present invention is achieved in that the sprout grafting method of Schisandra sphenanthera Rehd.et WilS, and the bud seedling that flattens before launching as yet to true leaf with cotyledon behind the seed germination of Schisandra sphenanthera Rehd.et WilS is a stock, is that grafting is carried out in scion with the green branch of Schisandra sphenanthera Rehd.et WilS, and grows up to new plant;
Described scion is the tender tip of semi-lignified young sprout, sublateral shoot or the tissue culture of Schisandra sphenanthera Rehd.et WilS;
Described grafting is that scion is grafted in the otch at stock hypocotyl place, and its method concrete steps are as follows:
(1) the bud seedling needs to cultivate in protections such as greenhouse or booth ground facility, and nutrition soil is the fertilizer that becomes thoroughly decomposed: river sand carefully: fertile rural area soil 5: 20: 75, and admix diammonium hydrogen phosphate in 0.3% ratio; The nutritive cube of packing into after sieving can adopt the plastics nutritive cube of 6cm * 6cm or 8cm * 8cm, and sowing Schisandra sphenanthera Rehd.et WilS seed is 3～5 in each nutritive cube, about thickness of earth covering 1cm; After planting, spray with 500 times of liquid of 50% carbendazol wettable powder in conjunction with watering; Temperature is controlled at 20～28 ℃, guarantees that nutrition soil is often moistening, and two cotyledons flatten after Schisandra sphenanthera Rehd.et WilS comes up, and true leaf can not carry out grafting before launching as yet;
(2) matrix of stock will be watered permeablely before the grafting, adopts the side grafting method promptly in hypocotyl place's grafting of stock, and the otch of stock is parallel with the direction of extension of two true leaves, obliquely cuts down, and the degree of depth is 1/2 of a hypocotyl diameter, and incision length is 0.4cm～0.6cm;
(3) simple bud or two bud grafting are adopted in scion, cut on the bud and stay about 0.5cm～1.0cm, cut under the bud and stay about 1.5cm～2.0cm, the scion lower end be whittled into the stock incision length quite or slightly long double inclined plane wedge shape, it is level and smooth that the inclined-plane is wanted, angle is little and even, guarantees at least one facing together behind the insertion stock otch, fixes with Grafting clip or fine rule bondage.
Good effect of the present invention is the product seedling of just turning out grafting then that has guaranteed the stock sowing, has reduced the grafting cost, has shortened the time limit of seedling-wood breeding; Improve the survival rate and the grafting efficiency of Schisandra sphenanthera Rehd.et WilS grafting, but large-scale application is produced in the grafting of Schisandra sphenanthera Rehd.et WilS; Also can promote simultaneously result ahead of time, and avoid the generation of creeping underground stem.
Embodiment
Below in conjunction with embodiment the present invention is further described:
Embodiment 1:
1, seed collecting and seed treatment
At 8 the end of month～mid-September, the ripening fruits of gathering is rubbed with the hands and is removed fruit pulp, floats and removes flat grain, puts shady and cool place and dries.The seed size of selected fruit will reach more than the thousand kernel weight 30g, and is sturdy neat to guarantee rootstock seedling, is convenient to the grafting operation and improves graft survival rate.Lamination processing period will be early than more than the sowing time 100d, earlier soak seed 3d during lamination, change water every day 1 time, mix with clean carefully river sand by 1: 3 the ratio seed that will wet then with clear water, the absolute water content of sand is 40%～50%, and temperature remains on 0 ℃～5 ℃.Seed is sifted out from lamination sand about first quarter moon before the sowing,, carry out disinfecting of seed, and place vernalization under 20 ℃～25 ℃ conditions, sow when the seed scarification about 50% or when exposing radicle with the carbendazim seed dressing of seed weight 0.2%～0.3%.
2, the bud seedling is cultivated
The bud seedling is cultivated in the greenhouse.Nutrition soil collocation method is that fertilizer becomes thoroughly decomposed: thin river sand: fertile rural area soil 5: 20: 75, and admix diammonium hydrogen phosphate in 0.3% ratio.After sieving nutrition soil packed into the plastics nutritive cube of 8cm * 8cm, sowing Schisandra sphenanthera Rehd.et WilS seed is 3～4 in each nutritive cube, about thickness of earth covering 1cm.After planting, spray with 500 times of liquid of 50% carbendazol wettable powder in conjunction with watering.Temperature is controlled at 25 ℃～27 ℃ in the facility, guarantees that nutrition soil is often moistening, carries out grafting when back two cotyledons flatten when Schisandra sphenanthera Rehd.et WilS comes up.
3 graftings
Grafting is carried out on the workbench of inside, greenhouse, and the matrix of grafting stock the previous day is watered permeable.Use sharp double-edged razor blade during grafting, will in time change about every blade grafting 150 strains.In the grafting process hand and grafting cutter will be repeatedly with 75% alcohol disinfecting, avoid cross-infection.Note simultaneously will drying just and can operating after the sterilization of hand and blade, otherwise otch gets wet or soup after healing difficulty.
Adopt the side grafting method, promptly in hypocotyl place's grafting of stock, otch is parallel with the direction of extension of two true leaves, oblique incision down, the degree of depth is 1/2 of a hypocotyl diameter, incision length is about 0.4cm～0.6cm, will there be certain distance the otch bottom with native face, prevents that otch from polluting, and influence survives.The semi-lignified young sprout of waiting to breed the kind robust growth is chosen in scion, and rugosity and stock are quite or slightly thick.After cutting, remove blade, only stay petiole.Scion preferably with adopting with usefulness, is transported as need at a distance, should carry out cooling, preserves moisture, fresh-keeping work, to improve survival rate.Adopt the simple bud grafting, cut on the bud and stay about 0.5cm～1.0cm, cut under the bud and stay about 1.0cm～1.5cm, the scion lower end be whittled into the stock incision length quite or slightly long double inclined plane wedge shape, it is level and smooth that the inclined-plane is wanted, and angle is little and even, guarantees at least one in the face of neat behind the insertion stock otch, fix every nutritive cube grafting 2 strains with the fine rule bondage.
4 managing after grafting
10～12d is the critical period of interface healing after the Schisandra sphenanthera Rehd.et WilS grafting, create conditions such as the humidity that helps wound healing, temperature, illumination, promotes the interface quickly-healing.
4.1 behind the moisture management Schisandra sphenanthera Rehd.et WilS sprout grafting, be placed in immediately in the little shed of relative moisture more than 90% of setting up in the greenhouse.Preceding 4d air humidity after the grafting will remain on more than 90%, and it is stuffy to close canopy 4d, selects the higher weather of temperature to ventilate 1 time every day in the morning or at dusk later on, notes the air humidity that keeps canopy interior.If in wound healing phase air humidity deficiency, need take the ground feedwater to increase humidity.In case of overcast and rainy, when humidity was big in the canopy, hydrofuge should in time leak informaton.Increase ventilation behind the 12d gradually, can remove little shed protection behind the 20d.
4.2 be 24～26 ℃ preference temperature daytime of temperature treatment grafting wound healing, is higher than 27 ℃ most; Be 20～22 ℃ night, minimumly is not less than 15 ℃, and general 10～12d can spend the grafting healing stage.12d is with rear venting, and progressively cooling keeps 22～24 ℃ daytime, 12～15 ℃ of nights.
4.3 4d wants comprehensive shading after the illumination management grafting, avoids the sunlight direct projection, adopts the shading screen shading of light transmittance 50% thereafter noon, sooner or later opens, and removes shading screen behind the 20d gradually, changes Routine Management over to.
About 7d, the young sprout more than the epicotyl be removed after the Schisandra sphenanthera Rehd.et WilS grafting, be needed running check later on and in time remove sprout tillers.The Schisandra sphenanthera Rehd.et WilS grafting can be practiced seedling about the 25d after the grafting, transplant about 30d in the open country nursery after the grafting.
5 nursery stock transplantings and nursery lot management
Wipe out the fine rule of bondage before the transplanting, grafting can be transplanted by the seeding row spacing band Tu Tuo of 10cm * 20cm on the seedling furrow of high 15cm, wide 1.2m, needs to adopt the shading screen of light transmittance 50% to shade after planting.7d recession removes shading screen, and at trench digging in the ranks, every square metre execute ammonium nitrate 20～25g, potassium sulphate 5～6g topdresses.August early and middle ten days carry out the 2nd time and topdress, execute diammonium hydrogen phosphate 30～40g, potassium sulphate 6～8g for every square metre, the fertilising back suitably increases waters number of times in order to growth of seedling.In the nursery, still want the running check sprout tillers also in time to erase, after the blade of scion growth has the autotrophy function, thoroughly wipe out the residual fraction of interface top stock.To note the generation of powdery mildew in the cultivation process, when finding powdery mildew is arranged, prevent and treat with 800～1000 times of liquid of triadimefon 25% wetting powder.
Embodiment 2:
1, seed collecting and seed treatment
At 8 the end of month～mid-September, the ripening fruits of gathering is rubbed with the hands and is removed fruit pulp, floats and removes flat grain, puts shady and cool place and dries.The seed size of selected fruit will reach more than the thousand kernel weight 30g, and is sturdy neat to guarantee rootstock seedling, is convenient to the grafting operation and improves graft survival rate.Lamination processing period will be early than more than the sowing time 100d, earlier soak seed 4d during lamination, change water every day 1 time, mix with clean carefully river sand by 1: 3 the ratio seed that will wet then with clear water, the absolute water content of sand is 40～50%, and temperature remains on 0～5 ℃.Seed is sifted out from lamination sand about first quarter moon before the sowing, place vernalization under 20～25 ℃ of conditions, the scarification of most of seed or expose radicle can be sowed.Prior to seeding, seed is handled with 600～800 times of liquid disinfectants of 50% iprodione.
2, the bud seedling is cultivated
The bud seedling is cultivated in booth.Nutrition soil collocation method is that fertilizer becomes thoroughly decomposed: thin river sand: fertile rural area soil 5: 20: 75, and admix diammonium hydrogen phosphate in 0.3% ratio.Sowing Schisandra sphenanthera Rehd.et WilS seed is 4～5 in the plastics nutritive cube of the 6cm * 6cm specification of packing into after sieving, each nutritive cube, about thickness of earth covering 1cm.After planting, spray with 500 times of liquid of 50% carbendazol wettable powder in conjunction with watering.Temperature in the booth is controlled at about 20～25 ℃, guarantees that nutrition soil is often moistening, and when Schisandra sphenanthera Rehd.et WilS comes up, two cotyledons flatten and carried out grafting in back 3 days before true leaf launches as yet.
3 graftings
Grafting is carried out on the workbench of booth inside, and the matrix of grafting stock the previous day is watered permeable.Use sharp double-edged razor blade during grafting, will in time change about every blade grafting 150 strains.Hand and grafting cutter will repeatedly be used 75% alcohol disinfecting in the grafting process, avoid cross-infection.Note simultaneously will drying just and can operating after the sterilization of hand and blade, otherwise otch gets wet or soup after healing difficulty.
Adopt the side grafting method, promptly in hypocotyl place's grafting of stock, otch is parallel with the direction of extension of two true leaves, oblique incision down, the degree of depth is 1/2 of a hypocotyl diameter, incision length is about 0.4cm～0.6cm, will there be certain distance the otch bottom with native face, prevents that otch from polluting, and influence survives.The semi-lignified sublateral shoot of waiting to breed the kind robust growth is chosen in scion.After cutting, remove blade, only stay petiole.During grafting, adopt the simple bud grafting, cut on the bud and stay about 0.5cm～1.0cm, cut under the bud and stay about 1.0cm～1.5cm, the scion lower end be whittled into the stock incision length quite or slightly long double inclined plane wedge shape, it is level and smooth that the inclined-plane is wanted, angle is little and even, guarantee at least one facing together after inserting the stock otch, fix every nutritive cube grafting 2 strains with the fine rule bondage.
4 managing after grafting
10～12d is the critical period of interface healing after the Schisandra sphenanthera Rehd.et WilS grafting, create conditions such as the humidity that helps wound healing, temperature, illumination, promotes the interface quickly-healing.
4.1 behind the moisture management Schisandra sphenanthera Rehd.et WilS sprout grafting, be placed in immediately in the little shed of relative moisture more than 90% of setting up in the greenhouse.Preceding 5d air humidity after the grafting will remain on more than 90%, and it is stuffy to close canopy 5d, selects the higher weather of temperature to ventilate 2 times every day in the morning or at dusk later on, notes the air humidity that keeps canopy interior.If in wound healing phase air humidity deficiency, should take the ground feedwater to increase humidity.In case of overcast and rainy, when humidity was big in the canopy, hydrofuge should in time leak informaton.Increase ventilation behind the 12d gradually, can remove little shed protection behind the 20d.
4.2 be 24～26 ℃ preference temperature daytime of temperature treatment grafting wound healing, is higher than 27 ℃ most; Be 20～22 ℃ night, minimumly is not less than 15 ℃, and general 10～12d can spend the grafting healing stage.12d is with rear venting, and progressively cooling keeps 22～24 ℃ daytime, 12～15 ℃ of nights.
4.3 4d wants comprehensive shading after the illumination management grafting, avoids the sunlight direct projection, adopts the shading screen shading of light transmittance 50% thereafter noon, sooner or later opens, and removes shading screen behind the 20d gradually, changes Routine Management over to.
About Schisandra sphenanthera Rehd.et WilS grafting 7d, the young sprout more than the epicotyl be removed, be needed running check later on and in time remove sprout tillers.The Schisandra sphenanthera Rehd.et WilS grafting can be practiced seedling about the 25d after the grafting, transplant about 30d in the open country nursery after the grafting.
5 nursery stock transplantings and nursery lot management
Wipe out the fine rule of bondage before the transplanting, grafting can be transplanted by the seeding row spacing band Tu Tuo of 10cm * 20cm on the seedling furrow of high 15cm, wide 1.2m, needs to adopt the shading screen of light transmittance 50% to shade after planting.7d recession removes shading screen, and at trench digging in the ranks, every square metre execute ammonium nitrate 20～25g, potassium sulphate 5～6g topdresses.August early and middle ten days carry out the 2nd time and topdress, execute diammonium hydrogen phosphate 30～40g, potassium sulphate 6～8g for every square metre, the fertilising back suitably increases waters number of times in order to growth of seedling.In the nursery, still want the running check sprout tillers also in time to erase, after the blade of scion growth has the autotrophy function, thoroughly wipe out the residual fraction of interface top stock.To note the generation of powdery mildew in the cultivation process, when finding that powdery mildew is arranged, prevent and treat with powder 1500～2000 times of liquid of peaceful 70% wetting powder that become rusty.
Embodiment 3:
1, seed collecting and seed treatment
At 8 the end of month～mid-September, the ripening fruits of gathering is rubbed with the hands and is removed fruit pulp, floats and removes flat grain, puts shady and cool place and dries.The seed size of selected fruit will reach more than the thousand kernel weight 30g, and is sturdy neat to guarantee rootstock seedling, is convenient to the grafting operation and improves graft survival rate.Lamination processing period will be early than more than the sowing time 100d, earlier soak seed 3d during lamination, change water every day 1 time, mix with clean carefully river sand by 1: 3 the ratio seed that will wet then with clear water, the absolute water content of sand is 40～50%, and temperature remains on 0～5 ℃.Seed is sifted out from lamination sand about first quarter moon before the sowing, place vernalization under 20～25 ℃ of conditions, the scarification of 40% seed or expose radicle can be sowed.Prior to seeding, seed is with 900 times of liquid of 70% mancozeb seed soaking 2h disinfection.
2, the bud seedling is cultivated
The bud seedling is cultivated in the greenhouse.Nutrition soil collocation method is that fertilizer becomes thoroughly decomposed: thin river sand: fertile rural area soil 5: 20: 75, and admix diammonium hydrogen phosphate in 0.3% ratio.After sieving nutrition soil is packed into the plastics nutritive cube of 8cm * 8cm, sowing Schisandra sphenanthera Rehd.et WilS seed is 3～4 in each nutritive cube, about thickness of earth covering 1cm.After planting, spray, with soil-borne diseases such as control damping offs with 500 times of liquid of 50% carbendazol wettable powder in conjunction with watering.Temperature is controlled at 20～25 ℃ in the facility, guarantees that nutrition soil is often moistening, after Schisandra sphenanthera Rehd.et WilS comes up, carries out grafting when true leaf does not launch as yet.
3 graftings
Grafting is carried out on the workbench of inside, greenhouse, and the matrix of grafting stock the previous day is watered permeable.Use sharp double-edged razor blade during grafting, will in time change about every blade grafting 150 strains.In the grafting process hand and grafting cutter will be repeatedly with 75% alcohol disinfecting, avoid cross-infection.Note simultaneously will drying just and can operating after the sterilization of hand and blade, otherwise otch gets wet or soup after healing difficulty.
Adopt the side grafting method, promptly in hypocotyl place's grafting of stock, otch is parallel with the direction of extension of two true leaves, oblique incision down, the degree of depth is 1/2 of a hypocotyl diameter, incision length is about 0.4cm～0.6cm, will there be certain distance the otch bottom with native face, prevents that otch from polluting, and influence survives.The tender tip that tissue culture is adopted in scion after cutting, removes blade, only stays petiole.Adopt two bud graftings, cut on the bud and stay about 0.5cm, cut under the bud and stay about 1.5cm, the scion lower end be whittled into the stock incision length quite or slightly long double inclined plane wedge shape, it is level and smooth that the inclined-plane is wanted, and angle is little and even, guarantees at least one in the face of neat behind the insertion stock otch, fix every nutritive cube grafting 2 strains with the fine rule bondage.
4 managing after grafting
10～12d is the critical period of interface healing after the Schisandra sphenanthera Rehd.et WilS grafting, create conditions such as the humidity that helps wound healing, temperature, illumination, promotes the interface quickly-healing.
4.1 behind the moisture management Schisandra sphenanthera Rehd.et WilS sprout grafting, be placed in immediately in the little shed of relative moisture more than 90% of setting up in the greenhouse.Preceding 4d air humidity after the grafting will remain on more than 90%, and it is stuffy to close canopy 4d, selects the higher weather of temperature to ventilate 2 times every day in the morning or at dusk later on, notes the air humidity that keeps canopy interior.If in wound healing phase air humidity deficiency, should take the ground feedwater to increase humidity.In case of overcast and rainy, when humidity was big in the canopy, hydrofuge should in time leak informaton.Increase ventilation behind the 12d gradually, can remove little shed protection behind the 20d.
4.2 be 24～26 ℃ preference temperature daytime of temperature treatment grafting wound healing, is higher than 27 ℃ most; Be 20～22 ℃ night, minimumly is not less than 15 ℃, and general 10～12d can spend the grafting healing stage.12d is with rear venting, and progressively cooling keeps 22～24 ℃ daytime, 12～15 ℃ of nights.
4.3 4d wants comprehensive shading after the illumination management grafting, avoids the sunlight direct projection, adopts the shading screen shading of light transmittance 50% thereafter noon, sooner or later opens, and removes shading screen after 20 days gradually, changes Routine Management over to.
About Schisandra sphenanthera Rehd.et WilS grafting 7d, the young sprout more than the epicotyl be removed, be needed running check later on and in time remove sprout tillers.The Schisandra sphenanthera Rehd.et WilS grafting can be practiced seedling about the 25d after the grafting, transplant about 30d in the open country nursery after the grafting.
5 nursery stock transplantings and nursery lot management
Wipe out the fine rule of bondage before the transplanting, grafting can be transplanted by the seeding row spacing band Tu Tuo of 10cm * 20cm on the seedling furrow of high 15cm, wide 1.2m, needs to adopt the shading screen of light transmittance 50% to shade after planting.7d recession removes shading screen, and at trench digging in the ranks, every square metre execute ammonium nitrate 20～25g, potassium sulphate 5～6g topdresses.August early and middle ten days carry out the 2nd time and topdress, execute diammonium hydrogen phosphate 30～40g, potassium sulphate 6～8g for every square metre, the fertilising back suitably increases waters number of times in order to growth of seedling.In the nursery, still want the running check sprout tillers also in time to erase, after the blade of scion growth has the autotrophy function, thoroughly wipe out the residual fraction of interface top stock.To note the generation of powdery mildew in the cultivation process, when finding powdery mildew is arranged, with 800～1000 times of liquid controls of thiophanate methyl wetting powder.
Claims (3)
1. the sprout grafting method of Schisandra sphenanthera Rehd.et WilS is characterized in that: the bud seedling that flattens before launching as yet to true leaf with cotyledon behind the Schisandra sphenanthera Rehd.et WilS seed germination is a stock, is that grafting is carried out in scion with the green branch of Schisandra sphenanthera Rehd.et WilS, and grows up to new plant.
2. the sprout grafting method of Schisandra sphenanthera Rehd.et WilS according to claim 1, it is characterized in that: described scion is the tender tip of semi-lignified young sprout, sublateral shoot or the tissue culture of Schisandra sphenanthera Rehd.et WilS;
3. the sprout grafting method of Schisandra sphenanthera Rehd.et WilS according to claim 1 is characterized in that: scion is grafted in the otch at stock hypocotyl place, its grafting method concrete steps are as follows:
(1) the bud seedling needs to cultivate in protections such as greenhouse or booth ground facility, and nutrition soil is the fertilizer that becomes thoroughly decomposed: river sand carefully: fertile rural area soil 5: 20: 75, and admix diammonium hydrogen phosphate in 0.3% ratio; The nutritive cube of packing into after sieving can adopt the plastics nutritive cube of 6cm * 6cm or 8cm * 8cm, and sowing Schisandra sphenanthera Rehd.et WilS seed is 3～5 in each nutritive cube, about thickness of earth covering 1cm; After planting, spray with 500 times of liquid of 50% carbendazol wettable powder in conjunction with watering; Temperature is controlled at about 20～28 ℃, guarantees that nutrition soil is often moistening, and two cotyledons flatten after Schisandra sphenanthera Rehd.et WilS comes up, and true leaf can not carry out grafting before launching as yet;
(2) matrix of stock will be watered permeablely before the grafting, adopts the side grafting method promptly in hypocotyl place's grafting of stock, and the otch of stock is parallel with the direction of extension of two true leaves, obliquely cuts down, and the degree of depth is 1/2 of a hypocotyl diameter, and incision length is 0.4cm～0.6cm;
(3) simple bud or two bud grafting are adopted in scion, cut on the bud and stay 0.5cm～1.0cm, cut under the bud and stay 1.5cm～2.0cm, the scion lower end be whittled into the stock incision length quite or slightly long double inclined plane wedge shape, it is level and smooth that the inclined-plane is wanted, angle is little and even, guarantees at least one facing together behind the insertion stock otch, fixes with Grafting clip or fine rule bondage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006101632095A CN101194570B (en) | 2006-12-06 | 2006-12-06 | Sprout grafting method for fructus schizandrae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006101632095A CN101194570B (en) | 2006-12-06 | 2006-12-06 | Sprout grafting method for fructus schizandrae |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101194570A true CN101194570A (en) | 2008-06-11 |
| CN101194570B CN101194570B (en) | 2010-12-08 |
Family
ID=39545340
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2006101632095A Expired - Fee Related CN101194570B (en) | 2006-12-06 | 2006-12-06 | Sprout grafting method for fructus schizandrae |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101194570B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102986331A (en) * | 2012-11-25 | 2013-03-27 | 溧阳市天目湖保健品有限公司 | Treatment method of seeds of fructus schisandrae |
| CN103703993A (en) * | 2013-12-30 | 2014-04-09 | 王燕 | Grafting growing method for peach blossom seedling |
| CN107637294A (en) * | 2017-10-31 | 2018-01-30 | 钟山佳园生态农业有限公司 | A kind of propagation by grafiting method of citrus fruit seedling |
| CN107646371A (en) * | 2017-10-31 | 2018-02-02 | 钟山佳园生态农业有限公司 | A kind of rapid propagation method of tribute mandarin orange fruit seedling |
| CN109729781A (en) * | 2019-03-06 | 2019-05-10 | 浙江省农业科学院 | A kind of cultural method of kadsura longepedunculata |
| CN114175934A (en) * | 2021-12-31 | 2022-03-15 | 吉林农业大学 | Green seedling grafting method for schisandra chinensis |
| CN114415760A (en) * | 2021-12-15 | 2022-04-29 | 国农生态农林 (江苏)有限公司 | Full-illumination spraying management system and management method and application thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1258434A (en) * | 1998-12-31 | 2000-07-05 | 衡东县林业局 | Cloning technology for high-yield tea-oil tree |
| CN1565161A (en) * | 2003-06-19 | 2005-01-19 | 东阳市森太农林果开发有限公司 | Whole-year grafting complete set technology for Chinese torreya sprout stock |
-
2006
- 2006-12-06 CN CN2006101632095A patent/CN101194570B/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102986331A (en) * | 2012-11-25 | 2013-03-27 | 溧阳市天目湖保健品有限公司 | Treatment method of seeds of fructus schisandrae |
| CN103703993A (en) * | 2013-12-30 | 2014-04-09 | 王燕 | Grafting growing method for peach blossom seedling |
| CN103703993B (en) * | 2013-12-30 | 2015-08-05 | 王燕 | A kind of method of peach bud seedling graft seedling growth |
| CN107637294A (en) * | 2017-10-31 | 2018-01-30 | 钟山佳园生态农业有限公司 | A kind of propagation by grafiting method of citrus fruit seedling |
| CN107646371A (en) * | 2017-10-31 | 2018-02-02 | 钟山佳园生态农业有限公司 | A kind of rapid propagation method of tribute mandarin orange fruit seedling |
| CN109729781A (en) * | 2019-03-06 | 2019-05-10 | 浙江省农业科学院 | A kind of cultural method of kadsura longepedunculata |
| CN114415760A (en) * | 2021-12-15 | 2022-04-29 | 国农生态农林 (江苏)有限公司 | Full-illumination spraying management system and management method and application thereof |
| CN114175934A (en) * | 2021-12-31 | 2022-03-15 | 吉林农业大学 | Green seedling grafting method for schisandra chinensis |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101194570B (en) | 2010-12-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103907473B (en) | Spring Melon grafting seedlings-growing method | |
| CN104081954B (en) | Paper mulberry cuttage propagation method | |
| CN1922987B (en) | Multiple-ear rock lithocarpus glaber dwarfing rapid reproduction method | |
| CN102972190A (en) | Method for grafting watermelons and cultivating grafted seedlings | |
| CN103960036A (en) | Method for conducting wild tending of Chinese Paris rhizome under trees | |
| CN101194570B (en) | Sprout grafting method for fructus schizandrae | |
| CN105340546A (en) | Cultivation method for cucumber grafted seedlings in greenhouse | |
| CN106034722A (en) | Cutting propagation method of golden leaf plant namely weigela florida variegata | |
| CN112602522A (en) | Method for grafting germ roots of small camellia oleifera seedlings | |
| CN105594438A (en) | Azalea seedling culture method | |
| CN106171467A (en) | Rhododendronsimiarum seed propagation method | |
| CN105594540A (en) | Camellia pubipetala grafting and seedling cultivating method | |
| CN107027627A (en) | A kind of micro-tuber propagation method of David's-harp IMMATURE EMBRYOS CULTURE | |
| CN101176431A (en) | Method for cultivating rapidly white potato breeder's seed | |
| CN109588126A (en) | A kind of acer fabri engrafting method | |
| CN106069552A (en) | A kind of oil tea asexual reproduction method | |
| CN106342555B (en) | Method for breeding new species of natural hetero-alternating heterogeneous populus euphratica and populus griseus | |
| CN104904555A (en) | Bonsai manufacturing method of seedling red maple | |
| CN103782789B (en) | Cultivation method for sonneratia apetala Buch-Ham seedlings | |
| CN103069980B (en) | Cultivation method of dalmatian chrysan themum | |
| CN102090229A (en) | Method for propagating female plants of Trichosanthes kirilowiiMaxim. by soilless culture substrate and plant growth cuttage | |
| CN106613363B (en) | A kind of cultural method of umbrella shape locust tree shade tree | |
| CN104272949A (en) | Crassulaceae plant cultivating and seedling method | |
| CN107258454A (en) | A kind of efficient implantation methods of nectarine cultivars in greenhouse | |
| CN109744040A (en) | A kind of cultivation of container seedling technology of the salt tolerant grafting rose of Sharon |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20101208 Termination date: 20131206 |