CN101176431A - Method for cultivating rapidly white potato breeder's seed - Google Patents
Method for cultivating rapidly white potato breeder's seed Download PDFInfo
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- CN101176431A CN101176431A CNA2007101800112A CN200710180011A CN101176431A CN 101176431 A CN101176431 A CN 101176431A CN A2007101800112 A CNA2007101800112 A CN A2007101800112A CN 200710180011 A CN200710180011 A CN 200710180011A CN 101176431 A CN101176431 A CN 101176431A
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Abstract
The invention discloses a planting method of breeder potato seed for reproducing potato rapidly in the current year. The virus-free test-tube plantlets of potato are planted in a tissue culture room during October to December and then transferred to a nutrition pot with nutrition stroma in greenhouse after the test-tube plantlets root. The temperature and the humidity of the greenhouse are controlled and the nutrition stroma is added into the nutrition pot after the plantlets come into three to four leaves. The potato mini tubers can be obtained after the plantlets grow in the nutrition pot for 50 to 60 days and then treated for dormance for 30 to 60 days after being graded. The potato mini tubers are sown into the soil of screen cage with insectproof screen after being treated for dormance and then field cared. The original cuttings are managed by ditching, earthing up, adding water and fertilizer after growing to 9 to 11 cm and the original cuttings can be harvested in October after growing for 4 to 5 months. The invention shortens the cycle of producing potato seeds and can obtain the breeder potato seed the current year.
Description
Technical field
The invention belongs to and carry out the cultivation method that potato tissue is cultivated breeding potato detoxicating breeder's stock production culture technique, particularly rapidly white potato breeder's seed.
Background technology
Potato is multiple virus of easy infection and viroids in the course of cultivation, produces complicated symptoms such as leaf roll, floral leaf, bunchy top dwarfing.Potato is an asexually propagated crop, and the virus in the body can cause viral degeneration and the serious underproduction by the generation accumulation, and underproduction amplitude is 40%, and serious reaches 70%.The Potyvirus kind is a lot, plants virus and a kind of viroids surplus the whole world has 30, what China was more general has 7 kinds, and as leaf curl, mosaic disease, spindle tuber etc., multiple virus is independent or compoundly cause various symptoms, seriously reduces the yield and quality of potato.The kind sexual involution of potato is not genetic variation, but cause by virus infection.
So-called " detoxification " sloughs the virus of potato oneself accumulation and infection exactly.The potato plant of infective virus, though body contains a large amount of virions, but the growing point meristematic tissue does not contain virus, cut potato young shoot stem apex vegetative cone 0.2-0.3 millimeter, carry out tissue culture, breed detoxification test tube plantlet, and then bear virus-free stem tuber, as virus-free potato seed, i.e. " virus-free seed potato ".This technology is called " stem apex detoxify " technology, and it has become the new way that prevents Potyvirus.Stem apex detoxify and virus-free potato seed have been bred now and combined, formed a complete detoxifying fast breeding for kind of a system.Detoxification test tube plantlet and micro potato are called detoxification core material or zero for potato, can be used for producing breeder's stock.Breeder's stock expanding propagation production one-level successively original seed and secondary original seed.Potato seed with the production of secondary original seed is the one-level breeding, and the one-level breeding breeds 1 once again, obtains the secondary breeding.In insect protected solarium or greenhouse, test-tube plantlet transplanted from test tube or cuttage in matrix such as perlite, peat, carry out soilless culture and produce little potato, breeder's stock production is except can be insect protected solarium or greenhouse carry out, also can be high and cold in physical features, aphid seldom, breed in the place that the isolation condition is good.500 meters on sowing ground does not have the Potyvirus host crop apart from the planted agent.The method of conventional potato primary stock potato is spring test-tube seedling transplanting to be gone down to receive autumn miniature little potato, carries out breeding of breeder's stock next spring again, and test-tube plantlet needed for two years to producing breeder's stock.
Summary of the invention
The object of the present invention is to provide a kind of cultivation method of rapidly white potato breeder's seed, can shorten the cycle of production potato seed, just can breed out the potato primary stock potato then.
The object of the present invention is achieved like this: a kind of cultivation method of rapidly white potato breeder's seed, and comprise and cultivate the potato detoxicating cuvette seedling, have the following steps:
1, cultivate the potato detoxicating cuvette seedling October-December in group training chamber, cut potato young shoot stem apex vegetative cone and cultivate detoxification test tube plantlet, 23-28 ℃ of group training indoor temperature, relative moisture 50-80%, illumination every day 12-16 hour was cultivated time 20-30 days;
2, the test-tube plantlet after will taking root moves into the greenhouse and is equipped with in the nutritive cube of nutrient matrix, and nutrient matrix is filled to 2/3 place in the nutritive cube, greenhouse temperature 10-30 ℃, relative moisture 30-70%;
3, the miniature little potato of hothouse production is taked to preserve moisture, and reduces gradually after sheltering from heat or light 5-7 days and shelters from heat or light, and ascending leaking informaton, when treating nutrition pot seedling length to 3-4 sheet leaf, add nutrient matrix to flat mouthful of nutritive cube in nutritive cube, nutrition pot seedling can be gathered in the crops miniature little potato after growing 50-60 days;
4, little potato dormancy changes dormancy over to and handles dormancy time 30-60 days after the little potato classification of results;
5, in the solarium, produce the breeder's stock potato, little potato after the dormancy processing end is broadcast in the solarium's soil with fly net, sowing line-spacing 70-80cm, spacing in the rows 15-20cm, degree of depth 7-8cm carries out field management, when the breeder's stock potato grows to 9-11cm, water and fertilizer management is strengthened in the trench digging ridging, and growth is results breeder's stock potato after 4-5 month.
The greenhouse room temperature of above-mentioned steps is 20-30 ℃, and relative moisture is 40-70%, and the composition of the nutrient matrix of dress is the mixture of sandy loam, peat soil and vermiculite in the nutritive cube, and the volume ratio of its component is 1: 1: 1.
Dormancy is treated to low temperature treatment or uses HORMONE TREATMENT.Low temperature treatment is that the athermobiosis environmental temperature of little potato is controlled at 1-2 ℃.HORMONE TREATMENT is for being the gibberellin even spraying of 10-50ppm on the surface of little potato with concentration, and the little potato after the spraying is stacked and got final product in 15-20 days.The solarium is the solarium, land for growing field crops with fly net.
Because the present invention is in 10-12 month, the toxicity-removing white potato test-tube plantlet is trained indoor the cultivation in group, temperature, humidity that control is examined, illumination fully, make test-tube plantlet propagation, expand numerous, take root, the test-tube plantlet after will taking root again moves into the greenhouse and is equipped with in the nutritive cube of nutrient matrix, Artificial Control greenhouse temperature and humidity, make test-tube plantlet higher in the content of organic matter, grow piece root (little potato) in the nutrient matrix of good permeability, reaching in time gathers after the maturing stage and enter resting stage handles.Dormancy is broadcast little potato into the solarium after finishing again, carries out field management and can gather in the crops the breeder's stock potato to autumn.Cultivation method of the present invention has shortened the time of production potato seed, just can breed out the potato primary stock potato in the cycle in 1 year.
Embodiment
1, cultivates the potato detoxicating cuvette seedling December in group training chamber, cut potato young shoot stem apex vegetative cone and cultivate detoxification test tube plantlet, 24-27 ℃ of group training indoor temperature, relative moisture 60-70%, illumination every day 12-14 hour, the test-tube plantlet that begins to take root, temperature treatment will widen day and night temperature gradually, open the test tube mouth of pipe after 5-7 days gradually, make test-tube plantlet adapt to greenhouse, the 30 days cultivation time of group training chamber test-tube plantlet;
2, the test-tube plantlet after will taking root moves into the greenhouse and is equipped with in the nutritive cube of nutrient matrix, and nutrient matrix is filled to 2/3 place in the nutritive cube, greenhouse temperature 12-28 ℃, relative moisture 50-70%;
3, the miniature little potato of hothouse production is taked to preserve moisture, and reduces gradually after sheltering from heat or light 5-7 days and shelters from heat or light, and ascending leaking informaton, when treating nutrition pot seedling length to 3-4 sheet leaf, add nutrient matrix to flat mouthful of nutritive cube in nutritive cube, nutrition pot seedling can be gathered in the crops miniature little potato after growing 50 days;
4, little potato dormancy changes athermobiosis over to and handles after the little potato classification of results, the environmental temperature of athermobiosis is controlled at 1-2 ℃, dormancy time 30-60 days;
5, in the solarium, produce the breeder's stock potato, little potato after the dormancy processing end is broadcast in the solarium's soil with fly net, sowing line-spacing 75cm, spacing in the rows 15cm, degree of depth 7-8cm carries out field management, when the breeder's stock potato grows to 9-11cm, the trench digging ridging was strengthened the water and fertilizer management growth after 5 months, can gather in the crops the breeder's stock potato to October in autumn.
Claims (5)
1. the cultivation method of a rapidly white potato breeder's seed comprises and cultivates the potato detoxicating cuvette seedling, it is characterized in that: have the following steps:
1. cultivate the potato detoxicating cuvette seedling October-December in group training chamber, 23-28 ℃ of group training indoor temperature, relative moisture 50-80%, illumination every day 12-16 hour was cultivated time 20-30 days;
2. the test-tube plantlet after will taking root moves into the greenhouse and is equipped with in the nutritive cube of nutrient matrix, and nutrient matrix is filled to 2/3 place in the nutritive cube, greenhouse temperature 10-30 ℃, relative moisture 30-70%;
3. the miniature little potato of hothouse production is taked to preserve moisture, and reduces gradually after sheltering from heat or light 5-7 days and shelters from heat or light, and ascending leaking informaton, when treating nutrition pot seedling length to 3-4 sheet leaf, add nutrient matrix to flat mouthful of nutritive cube in nutritive cube, nutrition pot seedling can be gathered in the crops miniature little potato after growing 50-60 days;
4. little potato dormancy changes dormancy over to and handles dormancy time 30-60 days after the little potato classification of results;
5. in the solarium, produce the breeder's stock potato, little potato after the dormancy processing end is broadcast in the solarium's soil with fly net, sowing line-spacing 70-80cm, spacing in the rows 15-20cm, degree of depth 7-8cm carries out field management, when the breeder's stock potato grows to 9-11cm, water and fertilizer management is strengthened in the trench digging ridging, and growth is results breeder's stock potato after 4-5 month.
2. the cultivation method of rapidly white potato breeder's seed according to claim 1 is characterized in that: the composition of nutrient matrix is the mixture of sandy loam, peat soil and vermiculite in the nutritive cube, and the volume ratio of its component is 1: 1: 1.
3. the cultivation method of rapidly white potato breeder's seed according to claim 1, it is characterized in that: dormancy is treated to low temperature treatment or uses HORMONE TREATMENT.
4. the cultivation method of rapidly white potato breeder's seed according to claim 3, it is characterized in that: low temperature treatment is that the athermobiosis environmental temperature of little potato is controlled at 1-2 ℃.
5. the cultivation method of rapidly white potato breeder's seed according to claim 3 is characterized in that: HORMONE TREATMENT was stacked 15-20 days for being the gibberellin even spraying of 10-50ppm on the surface of little potato with concentration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101800112A CN101176431A (en) | 2007-10-26 | 2007-10-26 | Method for cultivating rapidly white potato breeder's seed |
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| CNA2007101800112A CN101176431A (en) | 2007-10-26 | 2007-10-26 | Method for cultivating rapidly white potato breeder's seed |
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| CN101176431A true CN101176431A (en) | 2008-05-14 |
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| CNA2007101800112A Pending CN101176431A (en) | 2007-10-26 | 2007-10-26 | Method for cultivating rapidly white potato breeder's seed |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101810131A (en) * | 2010-05-07 | 2010-08-25 | 甘肃省敦煌种业股份有限公司玉门市种子公司 | Virus-free potato primary stock culture medium |
| CN102336602A (en) * | 2010-07-19 | 2012-02-01 | 甘肃省农业科学院马铃薯研究所 | Potato microtuber pellet substrate formula and preparation method thereof |
| CN105210600A (en) * | 2015-07-08 | 2016-01-06 | 甘肃凯凯农业科技发展股份有限公司 | Virus-free basic potato seed hot-house culture method |
| CN105724019A (en) * | 2016-02-24 | 2016-07-06 | 丽江市农业科学研究所 | Potato virus-free test-tube seedling direct transplanting method |
| CN105766385A (en) * | 2014-12-24 | 2016-07-20 | 姜汉军 | Plant protection method for increasing yield of virus-free potato breeder's stock |
| CN110226505A (en) * | 2019-07-12 | 2019-09-13 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | A method of utilizing micro potato young shoot numerous micro potato fastly |
| CN115088569A (en) * | 2022-06-13 | 2022-09-23 | 江苏宝德农业科技有限公司 | Breeding method of virus-free potato seeds |
-
2007
- 2007-10-26 CN CNA2007101800112A patent/CN101176431A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101810131A (en) * | 2010-05-07 | 2010-08-25 | 甘肃省敦煌种业股份有限公司玉门市种子公司 | Virus-free potato primary stock culture medium |
| CN101810131B (en) * | 2010-05-07 | 2011-05-04 | 甘肃省敦煌种业股份有限公司玉门市种子公司 | Detoxified potato primary stock culture medium |
| CN102336602A (en) * | 2010-07-19 | 2012-02-01 | 甘肃省农业科学院马铃薯研究所 | Potato microtuber pellet substrate formula and preparation method thereof |
| CN102336602B (en) * | 2010-07-19 | 2013-05-29 | 甘肃省农业科学院马铃薯研究所 | Potato microtuber pellet substrate formula and preparation method thereof |
| CN105766385A (en) * | 2014-12-24 | 2016-07-20 | 姜汉军 | Plant protection method for increasing yield of virus-free potato breeder's stock |
| CN105766385B (en) * | 2014-12-24 | 2019-05-10 | 庆阳敦博科技发展有限公司 | A kind of plant protection method improving virus-free basic potato seed yield |
| CN105210600A (en) * | 2015-07-08 | 2016-01-06 | 甘肃凯凯农业科技发展股份有限公司 | Virus-free basic potato seed hot-house culture method |
| CN105724019A (en) * | 2016-02-24 | 2016-07-06 | 丽江市农业科学研究所 | Potato virus-free test-tube seedling direct transplanting method |
| CN110226505A (en) * | 2019-07-12 | 2019-09-13 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | A method of utilizing micro potato young shoot numerous micro potato fastly |
| CN115088569A (en) * | 2022-06-13 | 2022-09-23 | 江苏宝德农业科技有限公司 | Breeding method of virus-free potato seeds |
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Open date: 20080514 |