CN110226505A - A method of utilizing micro potato young shoot numerous micro potato fastly - Google Patents
A method of utilizing micro potato young shoot numerous micro potato fastly Download PDFInfo
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- CN110226505A CN110226505A CN201910628999.7A CN201910628999A CN110226505A CN 110226505 A CN110226505 A CN 110226505A CN 201910628999 A CN201910628999 A CN 201910628999A CN 110226505 A CN110226505 A CN 110226505A
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- micro potato
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Soil Sciences (AREA)
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Abstract
The invention discloses a kind of methods using micro potato young shoot numerous micro potato fastly, and micro potato specific steps are as follows: micro potato vernalization: is put into GA3It is impregnated in solution, then moves into the young shoot that 25 DEG C of constant temperature illumination cultivation rooms are grown；Young shoot root induction: the young shoot grown is removed from micro potato bastem portion, moves into nutrient solution, until young shoot is taken root；Matrix prepares: matrix is mixed using vermiculite and perlite；New transplantation of seedlings: by new talent cuttage to seedbed, using natural lighting, according to nutrition status of the plant after slow seedling, water or nutrient solution are sprayed；After field planting 20 days, according to growth of cereal crop seedlings spray nutritious liquor；Keep matrix wet in new talent growth course；Micro potato harvest: micro potato can be harvested within 35~40 days to institute's growth of cuttage seedlings.The present invention has the seedling culture time short, high survival rate, while its breeding coefficient is high, and its is low in cost.
Description
Technical field
It is specifically a kind of fast using micro potato young shoot the present invention relates to a kind of mating system of micro potato
The method of numerous micro potato.
Background technique
China is the country of global potato (Solanumtuberosum L.) yield first, according to Chinese food industry
The data of potato Professional Committee, association, it is the fourth-largest after wheat, rice and corn to have become China for potato at present
Crop.
Currently, micro potato production is mainly removed using stem apex, using potato induced medium, detoxification is bred
Seedling, then fast breeding micro potato is achieved the purpose that by squamous subculture.
The Potato Shoot-tips used in industry at present repeat the method for cultivating detoxification, have applied for Chinese invention patent, specially
Sharp Publication No. CN107318656A, the patent use following steps: the potato tubers for needing detoxification a, being placed in surrounding screening
Germinated on soil in light, light-transmission top environment, the temperature after germination at 20 DEG C~25 DEG C carry out lifting culture；B, to be expedited the emergence of
Bud grows to 50~80cm, the stem apex of 0.8~1cm of clip on highest stem, and dish washing liquid water is cleaned after impregnating, and then soaks in clear water
It is rinsed well after bubble, aseptic water washing 4~5 times after then sterilizing, is inoculated on culture medium and is made into stem sharp；C, stem sharp is 25
DEG C~28 DEG C at a temperature of, illumination daily is cultivated for 12 hours under the intensity of illumination of 2200Lx~2500Lx；D, to stem sharp
When growing tall to 7~8cm, the stem apex of clip 0.2mm is placed in culture medium and is trained by the condition of culture of step c on the super-clean bench
It supports；E, clip stem apex conservation after repeating Step d 4~5 times, remaining stem section carry out viral diagnosis；F, after viral diagnosis is qualified, detoxification
It completes.
Above-mentioned technical method is implemented simple, and breeding coefficient is high, but its there is also following defects: due to using normal potato
Young shoot cultivates test tube seedling, needs by detoxification treatment, seedling culture growth time is long, and its survival rate is unstable, at high cost.
Summary of the invention
Micro potato young shoot numerous Ma Ling fastly is utilized in view of the above existing problems in the prior art, the present invention provides a kind of
The method of potato micro potato has the seedling culture time short, high survival rate, while its breeding coefficient is high, and its is low in cost.
To achieve the goals above, the technical solution adopted by the present invention is that: it is a kind of using micro potato young shoot it is numerous fastly
The method of micro potato, the specific steps of this method are as follows:
A, micro potato vernalization:
Micro potato is put into the GA that concentration is 10~15mg/L3It is small that 4~5 are impregnated in solution (i.e. Gibberellins solution)
When take out, then micro potato is using carbendazim processed sawdust covering, and moves into 25 DEG C of constant temperature illumination cultivation rooms, often
Day watering completes pregermination procedure when young shoot length that micro potato is grown is to 4~6cm；
B, young shoot root induction:
The young shoot grown in step A is removed from micro potato bastem portion, uses concentration for the NAA of 10~20mg/L
Hormone solution handles 15~25s or handles 20~40min with the IBA hormone solution that concentration is 40~60mg/L, then moves into
Clear water carries out water planting, nutrient solution is changed after 3 days, and be filled with oxygen into nutrient solution, promotes the rooting rate of young shoot, until young shoot is raw
New talent is formed after root completes the process；
C, matrix prepares:
Matrix is mixed using vermiculite and perlite according to the ratio of 2:1, is then filled the water to the matrix, is made containing for matrix
Water reaches 60%~70%；Matrix is deployed in seedbed；Matrix carries out boiling after potato harvest of every plantation
Disinfection；
D, new transplantation of seedlings:
By new talent cuttage obtained by step B to seedbed, seeding row spacing is 3.3 × 3.3cm, to be drenched the seedbed before cuttage, slow seedling
Phase does not water, and shades 5 days~10 days after cuttage, and temperature is controlled at 20 DEG C, and relative humidity is maintained at 95%~100%, using certainly
Right illumination sprays water or nutrient solution according to nutrition status of the plant after slow seedling；After field planting 20 days, 0.2 is sprayed according to the growth of cereal crop seedlings~
0.3% proportion is nutrient solution 4~6 times of N:P:K=2:1:3；Keep matrix wet in new talent growth course, water-holding capacity 60-
70%, when stolon, which has begun, to be expanded, soil humidity is controlled 75%~80%, 17 DEG C~25 DEG C of temperature, in conjunction with fertilising
Super matrix is trained, stopping in 7 days~10 days is watered before harvest；
E, micro potato harvests:
Micro potato (5~8g) can be harvested within growth of cuttage seedlings 35~40 days to step D institute.
Further, use concentration for 150~200mg/L bis- gill GGR (i.e. root-inducing powder) solution young shoot in the step B
Handle 20~30min.
Further, aqueous solution of the nutrient solution group as N:P:K=15:15:15 in the step B.
Further, the epidemic disaster in the step D is adjusted according to different bearing stage.
Further, the root length of new talent is 2~4cm in the step B.
Compared with prior art, the present invention has the advantage that
1, the sprouting cultivated using the present invention is sturdy, no longer needs to carry out the culture of strong seedling culture base, to reduce cost.
2, the new talent that takes root is obtained using the method that the seedling of step B of the present invention takes root, cuttage new talent can be greatly improved
Survival rate, and rootage duration is reduced, the superinfection of cuttage new talent is avoided, the breeding cycle of micro potato is shortened.
3, the breeding of micro potato is carried out using the present invention, it is only necessary to primary, gained micro potato can be proliferated within 5~6 weeks
It can be directly used for producing, it is numerous fastly to continue on for micro potato, and 5~6 times of growth coefficient；And gained micro potato is convenient for
Storage and transport.
Specific embodiment
The present invention will be further described below.
A method of utilizing micro potato young shoot numerous micro potato fastly, the specific steps of this method are as follows:
A, micro potato vernalization:
Micro potato is put into the GA that concentration is 10~15mg/L3It is small that 4~5 are impregnated in solution (i.e. Gibberellins solution)
When take out, then micro potato is using carbendazim processed sawdust covering, and moves into 25 DEG C of constant temperature illumination cultivation rooms, often
Day watering completes pregermination procedure when young shoot length that micro potato is grown is to 4~6cm；
B, young shoot root induction:
The young shoot grown in step A is removed from micro potato bastem portion, uses concentration for the NAA of 10~20mg/L
Hormone (i.e. methyl α-naphthyl acetate) solution handles 15~25s or IBA hormone (i.e. indolebutyric acid) solution with concentration for 40~60mg/L
20~40min is handled, clear water is then moved into and carries out water planting, nutrient solution is changed after 3 days, and be filled with oxygen into nutrient solution, promote children
The rooting rate of bud, until young shoot, which forms new talent after taking root, completes the process；
C, matrix prepares:
Matrix is mixed using vermiculite and perlite according to the ratio of 2:1, is then filled the water to the matrix, is made containing for matrix
Water reaches 60%~70%；Matrix is deployed in seedbed；Matrix carries out boiling after potato harvest of every plantation
Disinfection；
D, new transplantation of seedlings:
By new talent cuttage obtained by step B to seedbed, seeding row spacing is 3.3 × 3.3cm, to be drenched the seedbed before cuttage, slow seedling
Phase does not water, and shades 5 days~10 days after cuttage, and temperature is controlled at 20 DEG C, and relative humidity is maintained at 95%~100%, using certainly
Right illumination sprays water or nutrient solution according to nutrition status of the plant after slow seedling；After field planting 20 days, 0.2 is sprayed according to the growth of cereal crop seedlings~
0.3% proportion is nutrient solution 4~6 times of N:P:K=2:1:3；Keep matrix wet in new talent growth course, water-holding capacity 60-
70%, when stolon, which has begun, to be expanded, soil humidity is controlled 75%~80%, 17 DEG C~25 DEG C of temperature, in conjunction with fertilising
Super matrix is trained, stopping in 7 days~10 days is watered before harvest；
E, micro potato harvests:
Micro potato (5~8g) can be harvested within growth of cuttage seedlings 35~40 days to step D institute.
As an improvement of the present invention, use concentration for 150~200mg/L bis- gill GGR young shoot in the step B
(i.e. root-inducing powder) solution handles 20~30min；Nutrient solution group becomes the aqueous solution of N:P:K=15:15:15 in the step B；Institute
The epidemic disaster stated in step D is adjusted according to different bearing stage；The root length of new talent is 2~4cm in the step B.
Claims (5)
1. a kind of method using micro potato young shoot numerous micro potato fastly, which is characterized in that this method it is specific
Step are as follows:
A, micro potato vernalization:
Micro potato is put into the GA that concentration is 10~15mg/L3It impregnates 4~5 hours and takes out in solution, then potato is micro-
Type potato moves into 25 DEG C of constant temperature illumination cultivation rooms using the processed sawdust covering of carbendazim, daily to water, miniature to potato
When the young shoot length that potato is grown is to 4~6cm, pregermination procedure is completed；
B, young shoot root induction:
The young shoot grown in step A is removed from micro potato bastem portion, uses concentration for the NAA hormone of 10~20mg/L
Solution handles 15~25s or handles 20~40min with the IBA hormone solution that concentration is 40~60mg/L, then moves into clear water
Water planting is carried out, nutrient solution is changed after 3 days, and be filled with oxygen into nutrient solution, promotes the rooting rate of young shoot, until after young shoot is taken root
It forms new talent and completes the process；
C, matrix prepares:
Matrix is mixed using vermiculite and perlite according to the ratio of 2:1, is then filled the water to the matrix, is made the water content of matrix
Reach 60%~70%；Matrix is deployed in seedbed；
D, new transplantation of seedlings:
By new talent cuttage obtained by step B to seedbed, seeding row spacing is 3.3 × 3.3cm, to be drenched the seedbed before cuttage, seedling-slowing stage is not
Watering, shades 5 days~10 days after cuttage, and temperature is controlled at 20 DEG C, and relative humidity is maintained at 95%~100%, using natural light
According to spraying water or nutrient solution according to nutrition status of the plant after slow seedling；After field planting 20 days, 0.2~0.3% is sprayed according to the growth of cereal crop seedlings and is matched
Than nutrient solution 4~6 times for N:P:K=2:1:3；Keep matrix wet in new talent growth course, water-holding capacity 60-70%, when
Stolon has begun when expanding, and soil humidity control is 75%~80%, 17 DEG C~25 DEG C of temperature, in conjunction with fertilising Pei Gaoji
Matter, stopping in 7 days~10 days is watered before harvest；
E, micro potato harvests:
Micro potato can be harvested within growth of cuttage seedlings 35~40 days to step D institute.
2. a kind of method using micro potato young shoot numerous micro potato fastly according to claim 1, special
Sign is, uses concentration to handle 20~30min for the bis- gill GGR solution of 150~200mg/L young shoot in the step B.
3. a kind of method using micro potato young shoot numerous micro potato fastly according to claim 1, special
Sign is that nutrient solution group becomes the aqueous solution of N:P:K=15:15:15 in the step B.
4. a kind of method using micro potato young shoot numerous micro potato fastly according to claim 1, special
Sign is that the epidemic disaster in the step D is adjusted according to different bearing stage.
5. a kind of method using micro potato young shoot numerous micro potato fastly according to claim 1, special
Sign is that the root length of new talent is 2~4cm in the step B.
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| CN201910628999.7A CN110226505A (en) | 2019-07-12 | 2019-07-12 | A method of utilizing micro potato young shoot numerous micro potato fastly |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111448962A (en) * | 2020-05-06 | 2020-07-28 | 云南泵龙马铃薯种植有限公司 | Breeding method of virus-free miniature potatoes |
| CN111528097A (en) * | 2020-06-09 | 2020-08-14 | 吉林省蔬菜花卉科学研究院 | Method for breeding potato mini-potatoes by utilizing stem segments of potato stolons |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111448962A (en) * | 2020-05-06 | 2020-07-28 | 云南泵龙马铃薯种植有限公司 | Breeding method of virus-free miniature potatoes |
| CN111528097A (en) * | 2020-06-09 | 2020-08-14 | 吉林省蔬菜花卉科学研究院 | Method for breeding potato mini-potatoes by utilizing stem segments of potato stolons |
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Application publication date: 20190913 |