CN1154413C - Amorphophallus rivieri group seedling-culturing batch production and cultivation technology - Google Patents
Amorphophallus rivieri group seedling-culturing batch production and cultivation technology Download PDFInfo
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- CN1154413C CN1154413C CNB991210514A CN99121051A CN1154413C CN 1154413 C CN1154413 C CN 1154413C CN B991210514 A CNB991210514 A CN B991210514A CN 99121051 A CN99121051 A CN 99121051A CN 1154413 C CN1154413 C CN 1154413C
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Abstract
The present invention relates to a technique for the batch production of amorphophallus rivieri tissue culture seedlings and the cultivation of the tissue culture seedlings, which belongs to a technique for the cultivation of crops. In the present invention, identical culture mediums are adopted, the inoculation and the secondary culture are carried out according the operating conditions publicly known, and seedlings can be formed only after 45 days and through one step. The secondary culture is carried out for one time every twenty days, and the proliferation multiple is 1:4 or 1:6. Roots can be generated within 12 to 15 days during the process of rooting culture. Young seedlings with the cleaning culture stems of roots are put in a seedbed, the young seedlings grow under the conditions of the temperature of 20 to 30 DEG C, the humidity of 80 to 90% and the sun-shading degree of 50%, and paclobutrazol of 1 to 6 ppm and a nutrient solution are applied. The present invention has the advantages of one-step seedling forming, multiple seeding with one seedling, short growth cycle, low cost, etc. The mass production of the tissue culture seedlings can be realized, and more than 50g of seed amorphophallus rivieris can be obtained in the same year.
Description
Technical field:
The present invention relates to mass production of a kind of konjaku tissue cultivating seedling and tissue cultivating seedling culture technique, belong to agro-ecology and agricultural cultivation
Technical field.
Background technology:
Because contained Glucomannan has extensive, special purposes in the konjaku, causes the interest and the attention of many countries.From the end of the eighties, constantly the someone adopts two to go on foot into the work that the seedling method is cooked the konjaku tissue culture, and to influence propagation in the konjaku group training process, various factors such as take root studies, and a small amount of tissue cultivating seedling is moved into field production, and obtained taro kind about 8～30g, and the stem tuber that is used to produce (planting taro) needs the vegetative period in 3 years.Above-mentioned test only is confined to laboratory research, and experimentation cost is higher.Up to now, do not see and can realize mass production konjaku tissue cultivating seedling, and can gather in the crops the stem tuber weight average more than 50g then, a young plant can be produced the open report of a big stem tuber, 2～4 little taros.
Summary of the invention:
The objective of the invention is to overcome the deficiency of prior art, provide a kind of and can realize forming seedling through one step culture, a seedling is multiple, growth cycle is short, cost is low, and can be when producing konjaku tissue cultivating seedling mass and the tissue cultivating seedling culture technique of taro (stem tuber) more than the average weight 50g per year.
Mass production of konjaku tissue cultivating seedling and tissue cultivating seedling culture technique are made up of operations such as breeding stem tuber in konjaku explant, flushing, sterilization, inoculation, successive transfer culture, culture of rootage and seedling bottle outlet, the seedbed.The present invention adopts inoculation and successive transfer culture operation all to use forming seedling through one step culture method with a kind of medium, promptly earlier with tuberous konjaku explant flushing, and be that specification is got the terminal bud part by 0.5cm * 0.5cm, soaked 15 minutes with 75% alcohol-pickled 2～3 seconds and 0.1% mercuric chloride respectively again, use aseptic water washing immediately, the konjaku stem tuber explant after the flushing is at medium MS+6BA
6～0.1Mg/L+NAA
6～0Among the mg/LPH5.8, once-seedling forming after temperature is to cultivate 45 days in the environment of, illumination 1000LX on 20 ℃, successive transfer culture every 20～25 days subcultures once, the propagation multiple is 1: 4～1: 6, the temperature of successive transfer culture is 20 ℃ and goes up with, illumination 1000LX.Culture of rootage is to divide individual plant to cut open with grow to the high seedling that grows thickly of 3～4cm in successive transfer culture, puts into MS+NAA
5～1In the medium of mg/L 12～15 days is bottle outlet after sending out roots, and control temperature and illumination are identical with the successive transfer culture process.The seedling of bottle outlet is cleaned the medium of seedling root earlier with 0.1% carbendazim solution, subsequently seedling is moved to and breed stem tuber (planting taro) in the seedbed of seeding row spacing 15cm * 15cm, in temperature is 20 ℃～30 ℃, humidity is 80%～90%, shade density is to grow under 50% the condition, and changing paclobutrazol and the nutrient solution that applies 1～6ppm seedling stage, the ratio of nutrient solution is N: P: K: Ca: Mg=5: 1: 5: 0.5: 1, wherein the every two weeks of nutrient solution applies once, applied once in per 4～5 days at the stem tuber expanding stage, until the stem tuber maturation, average weight reached more than the 50g when tissue cultivating seedling stem tuber of transplant survival was ripe, and most of stem tuber grows 2～4 root-like stocks again.The controlled condition of above-mentioned successive transfer culture and culture of rootage is same as the prior art.
The present invention compared with prior art has in inoculation and the subculture process and adopts with a kind of medium, can realize forming seedling through one step culture, and a seedling is multiple, and growth cycle is short, and cost is low, and can realize the mass production tissue cultivating seedling and obtain more than the 50g advantage such as kind taro then.
Embodiment:
Earlier with tuberous konjaku explant flushing, and by 0.5cm * 0.5cm is that specification is got the terminal bud part, soaks 15 minutes with 75% alcohol-pickled 2～3 seconds and 0.1% mercuric chloride respectively again, uses aseptic water washing immediately, and the konjaku stem tuber explant after the flushing is put into MS+6BA
6～0.1Mg/L+NAA
6～0In mg/L, the PH5.8 medium, be more than 20 ℃, cultivate in the environment of illumination 1000LX and got final product forming seedling through one step culture in 45 days in temperature.Put into tissue cultivating seedling same medium again and carry out successive transfer culture this moment, and every 20 days subcultures once, the propagation multiple is 1: 4～1: 6.Temperature in the successive transfer culture process is more than 20 ℃, illumination is 1000LX.When seedling grows to 3～4cm when high, carry out the plant division cutting, put into MS+NAA
5～1Culture of rootage is 12～15 days in the root media of mg/L, is bottle outlet after sending out roots, and control temperature and illumination are identical with the successive transfer culture process.Before the transplanting, earlier the medium of seedling root being cleaned with 0.1% carbendazim solution, subsequently seedling is moved in the seedbed of seeding row spacing 15cm * 15cm and breed stem tuber (kind taro), is 20 ℃～30 ℃ in temperature, humidity is 80%～90%, and shade density is to grow under 50% the condition.And changing paclobutrazol and the nutrient solution that applies 1～6ppm seedling stage, and the ratio of nutrient solution is N: P: K: Ca: Mg=5: 1: 5: 0.5: 1, and wherein the every two weeks of nutrient solution applies once, applies once in per 4～5 days at the stem tuber expanding stage, until the stem tuber maturation.Weight was general all more than 50 grams when stem tuber was ripe, and several taros grow 2～4 root-like stocks again mostly, enlarged reproduction coefficient, reduced cost.The tissue cultivating seedling that present technique is produced is the kind taro with regard to obtaining to can be used for producing in two years.
Claims (1)
1. konjaku tissue cultivating seedling mass production and tissue cultivating seedling culture technique are formed by breeding the stem tuber operation in konjaku explant, flushing, sterilization, inoculation, successive transfer culture, culture of rootage and seedling bottle outlet, the seedbed, it is characterized in that:
1.1 employing inoculation and successive transfer culture operation are all used the forming seedling through one step culture method with a kind of medium, promptly earlier with tuberous konjaku explant flushing, and be that specification is got the terminal bud part by 0.5cm * 0.5cm, soaked 15 minutes with 75% alcohol-pickled 2～3 seconds and 0.1% mercuric chloride respectively again, use aseptic water washing immediately, the konjaku stem tuber explant after the flushing is at medium MS+6BA
6～ 0.1Mg/L+NAA
6～0Among the mg/L PH5.8, once-seedling forming after temperature is to cultivate 45 days in the environment of, illumination 1000LX on 20 ℃, successive transfer culture every 20～25 days subcultures once, the propagation multiple is 1: 4～1: 6, the temperature of successive transfer culture is 20 ℃ and goes up with, illumination 1000LX;
1.2 culture of rootage is to divide individual plant to cut open with grow to the high seedling that grows thickly of 3～4cm in successive transfer culture, puts into MS+NAA
5～1In the medium of mg/L 12～15 days is bottle outlet after sending out roots, and control temperature and illumination are identical with the successive transfer culture process;
1.3 the seedling of bottle outlet is cleaned the medium of seedling root earlier with 0.1% carbendazim solution, subsequently seedling is moved in the seedbed of seeding row spacing 15cm * 15cm and breed stem tuber, in temperature is 20 ℃～30 ℃, humidity is 80%～90%, shade density is to grow under 50% the condition, and changing paclobutrazol and the nutrient solution that applies 1～6ppm seedling stage, the ratio of nutrient solution is N: P: K: Ca: Mg=5: 1: 5: 0.5: 1, wherein the every two weeks of nutrient solution applies once, applied once in per 4～5 days at the stem tuber expanding stage, until the stem tuber maturation, average weight reached more than the 50g when stem tuber was ripe, and most of stem tuber grows 2～4 root-like stocks again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB991210514A CN1154413C (en) | 1999-11-14 | 1999-11-14 | Amorphophallus rivieri group seedling-culturing batch production and cultivation technology |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB991210514A CN1154413C (en) | 1999-11-14 | 1999-11-14 | Amorphophallus rivieri group seedling-culturing batch production and cultivation technology |
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| CN1295790A CN1295790A (en) | 2001-05-23 |
| CN1154413C true CN1154413C (en) | 2004-06-23 |
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| CNB991210514A Expired - Fee Related CN1154413C (en) | 1999-11-14 | 1999-11-14 | Amorphophallus rivieri group seedling-culturing batch production and cultivation technology |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101011029B (en) * | 2007-02-06 | 2010-12-22 | 湖北省农业科学院经济作物研究所 | Method for building konjak suspension culture system |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100355333C (en) * | 2004-09-17 | 2007-12-19 | 中国科学院昆明植物研究所 | Bulbil konjak sprout breeding method |
| CN100574611C (en) * | 2007-12-04 | 2009-12-30 | 云南省农业科学院生物技术与种质资源研究所 | A kind of method for breeding konjak fine breed |
| CN102640650B (en) * | 2012-04-25 | 2014-03-12 | 云南省农科院富源魔芋研究所 | Konjac cultivation method |
| CN103430822B (en) * | 2013-09-09 | 2015-03-18 | 云南省农业科学院生物技术与种质资源研究所 | Aquaculture seed reproduction method for micro seed tubers of konjac |
| CN106718744A (en) * | 2016-12-10 | 2017-05-31 | 许连峰 | A kind of konjaku bottom set fast seedling-cultivating method |
| CN107318305B (en) * | 2017-08-18 | 2020-12-25 | 安康学院 | Rapid breeding method for konjac seedling seeds |
| CN108184588B (en) * | 2018-01-16 | 2020-06-30 | 安康市农业科学研究所 | Efficient transplanting method for konjac tissue culture seedlings |
| CN113973717A (en) * | 2021-11-29 | 2022-01-28 | 云南省热带作物科学研究所 | Germination accelerating and seedling raising method for bulbil konjak tissue culture micro-corms |
| CN115191348B (en) * | 2022-07-28 | 2023-06-06 | 安康市农业科学研究院 | Batch induction method of konjak mutants |
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1999
- 1999-11-14 CN CNB991210514A patent/CN1154413C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101011029B (en) * | 2007-02-06 | 2010-12-22 | 湖北省农业科学院经济作物研究所 | Method for building konjak suspension culture system |
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