CN103430822A - Aquaculture seed reproduction method for micro seed tubers of konjac - Google Patents
Aquaculture seed reproduction method for micro seed tubers of konjac Download PDFInfo
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- CN103430822A CN103430822A CN2013104071976A CN201310407197A CN103430822A CN 103430822 A CN103430822 A CN 103430822A CN 2013104071976 A CN2013104071976 A CN 2013104071976A CN 201310407197 A CN201310407197 A CN 201310407197A CN 103430822 A CN103430822 A CN 103430822A
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- taro
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Abstract
The invention discloses an aquaculture seed reproduction method for micro seed tubers of konjac, belonging to the technical field of agriculture breeding and propagation. The aquaculture seed reproduction method comprises the steps of segmenting nodular calluses of a petiole and a base thereof of a konjac tissue culture seedling by using a knife, wherein the konjac tissue culture seedling grows until reaching the height of 4-5cm and does not root; washing the tissue culture seedling with water and wrapping the basal of the tissue culture seedling with sponge, planting in a hole plug and carrying out water-planting indoors by using tap water, spraying a nutrient solution once every 7 days after the tissue culture seedling grows for 7 days, wherein the mass ratio of N to P to K to Ca to Mg in the nutrient solution is 23:1:21:4:1, the total concentration of the nutrient solution is 225mg/L, the indoor temperature at night is 10-15 DEG C, the indoor temperature in the daylight is 20-25 DEG C, and the illumination is scattered light; carrying out water-planting for 3 months and then stopping water-planting to ensure that plants naturally suffers from sprout tumble, namely, the mature micro seed tubers can be harvested. According to the method, the micro seed tubers with the weight of 1.5g on average of the konjac can be bred, thus the propagation period of excellent seed tubers of the konjac is greatly shortened, the production cost is low, and the survival rate and the tuber yield reach 85-90 percent.
Description
Technical field
The present invention relates to miniature kind of taro water planting breeding technique of a kind of konjaku, belong to agricultural breeding and raising technology field.
Technical background
Konjaku (konjac) is Araeceae Amorphophallus herbaceos perennial, mainly with bulb, carry out vegetative propagation, konjaku can and be transplanted to seedbed by Plant Tissue Breeding biotechnology bred good seed at present and carry out stock breeding, and effective solution is provided for konjaku kind sexual involution, good species taro are in great shortage.But the cultivation management of konjaku group training seedling is a very meticulous job, technical operation and cultivation condition are not slightly noted survival rate and the knot taro rate of the training of reduction group greatly seedling, and the cycle of production high-quality kind taro is longer, needs a growth cycle (6-8 months).
Therefore, find a kind of breeding cycle that can shorten miniature kind of taro of konjaku, the method that is easy to again to breed is that this area scientific and technical personnel want the technical problem of exploring for a long time.Up to now, have no and breed the open report of miniature kind of taro of konjaku by water culture technology within the shorter time (3 months).
Summary of the invention:
The technical problem to be solved in the present invention is that the cultivation management technology operation and the cultivation condition that overcome the fast middle konjaku group training seedling of miniature kind of taro group of existing konjaku training require height, easily cause survival rate and the knot taro rate of group training seedling greatly to reduce, and longer technological deficiency and the deficiency of cycle of production high-quality kind taro.Its objective is a kind of breeding cycle that can shorten konjaku high-quality kind taro is provided, breeding operation is easy again, is easy to survive, can reduce the method for the production cost of high-quality kind taro.
For solving the problems of the technologies described above and reaching the object of the invention, the invention provides the water planting breeding method of miniature kind of taro of a kind of konjaku, the method is carried out Plant Tissue Breeding by prior art to konjaku, it is characterized in that:
(1) the konjaku group of the not taking root training seedling cutting that the height of seedling of Plant Tissue Breeding being produced is 4cm-5cm, cutting is that the warty callus of the petiole of this seedling and its base portion is separated, then after rinsing with running water the medium that removes group training seedling base portion, with sponge wrapping group training seedling base portion, replanting in the dish of cave uses running water in indoor water planting, group training seedling hydroponics growing is after 7 days, sprayed N:P:K:Ca:Mg mass ratio 23:1:21:4:1 one time every 7 days, the nutrient solution that total concentration is 225mg/L, during water planting in control room nocturnal temperature be 10 ℃～15 ℃, day temperature is 20 ℃～25 ℃, illumination is scattered light,
(2) water planting is no longer carried out water planting in 3 months, allows plant naturally fall seedling, can gather in the crops miniature kind of ripe taro.
The plant square that described group of training seedling of step (1) implanted in the dish of cave is 5.2cm * 5.2cm.
Compared with prior art, the beneficial effect of the inventive method is:
1, the inventive method has shortened the breeding cycle of konjaku high-quality kind taro greatly, and the breeding cycle has shortened the time more than 1 times.
The inventive method is groped by a large amount of tests, obtained the technical scheme of miniature kind of taro of water planting breeding konjaku, this technical scheme is in the cutting of its group training seedling, the control of diurnal temperature, under the comprehensive cooperation of nutrient solution key technology means, make konjaku group training seedling just can cultivate miniature kind of taro of konjaku of average 1.5 grams in the trimestral time, and in miniature kind of taro tissue-culturing rapid propagation of existing konjaku, after seedling is taken root in the training of formation konjaku group, train from the konjaku group seedling hardening of taking root, transplant to cultivating into miniature kind of taro of konjaku and need the growth cycle of 6-8 months, could gather in the crops miniature kind of taro of konjaku, therefore, the inventive method has shortened the breeding cycle of konjaku high-quality kind taro greatly, its breeding cycle has shortened the time more than 1 times.
2, the inventive method is easy and simple to handle, and survival rate and knot taro rate high (survival rate reaches 85-90%, survives and ties the taro rate), production cost are low.The inventive method only needs running water, nutrient solution cheaply, control diurnal temperature and cave dish water planting instrument commonly used, easy and simple to handle being easy to of these working conditions realized, cost is low, overcome the take root cultivation management conditional request of training seedling of existing konjaku group high, slightly do not noted greatly reducing the take root survival rate of group training seedling and the defect of knot taro rate.
3, the inventive method can repeat 4 cycles in 1 year easily, can gather in the crops miniature kind of taro of 4 batches of konjakus, has improved the coefficient of breeding of planting taro.
Embodiment
The at present existing plant tissue culture technique of embodiment 1 carries out Plant Tissue Breeding to konjaku
Konjaku is carried out to Plant Tissue Breeding and can adopt following steps:
(1) explant jigs, and jigs that to physically well develop, plant skin harmless, smooth, spherical slightly ellipse, the bud nest is more shallow, the konjak corm of the about 250g of weight left and right is explant;
(2) cleaning of explant and vernalization,, stop in 28 ℃ of cleaning ambients and carry out the vernalization processing explant (bulb) epidermis and clear Xian of bud nest with running water;
(3) grow to 2～3cm when the konjak corm terminal bud, cut terminal bud, by the routine disinfection method to the material disinfection, described routine disinfection method can be carried out by the following method: the terminal bud cut with clear Xian of running water, 5 minutes → aseptic water washing of the mercury chloride that 1 second of alcohol surface sterilization → mass fraction is 0.1% that is 70% with mass fraction sterilization 3 times → under aseptic condition, strip growing point, it is seeded on the medium prepared, induce and differentiate seedling with existing tissue culture technique, described existing tissue culture technique is induced and is differentiated seedling and can carry out by the following method:
1. induce cultivation: above-mentioned sterile-processed material is inoculated in to medium: MS+6-BA0.5mg/L+NAA0.5mg/L+ sucrose 40g/L+ agar 6.5g/L, pH=5.8, cultivation temperature is 22-25 ℃, and light application time is 9h/d, and intensity of illumination is 1500lx; Can form the warty callus through the cultivations of 30 days;
2. differentiation is cultivated: above-mentioned warty callus is proceeded in differential medium, break up cultivation, differential medium: MS+6-BA0.2mg/L+NAA1.0mg/L+ sucrose 30g/L+ agar 6.5g/L, pH=5.8, cultivation temperature is 22-25 ℃, and light application time is 12h/d, and intensity of illumination is 2000lx; Differentiation is cultivated just can differentiate seedling in 20 days;
3. grow to 4-5 centimeters high when seedling, and while not taking root, can carry out the water planting breeding method by the described method of embodiment 1.
The water planting breeding method of miniature kind of taro of embodiment 2 konjakus
(1) water planting is carried out indoor, first will cultivate the konjaku group of the not taking root training seedling cutting that the height of seedling produced is 4cm-5cm by conventional organization, during cutting, with sharp scalpel, the warty callus of the petiole of this seedling and its base portion is separated, then wash away the medium of this group training seedling base portion with running water, the carbendazim that is 5% with mass fraction soaks 2-3 minutes, wrap up the plant strain base portion with sponge as fixture, implanting seeding row spacing is in 5.2 centimeters * 5.2 centimeters cave dishes, each cave dish plantation 100 strain, this cave dish is positioned on the water surface of the container (dish) that fills running water, then this container (dish) is placed on desk-top culturing rack, between between every layer of table top, square is 35 centimeters-40 centimeters, during water planting, indoor nocturnal temperature is controlled at 10～15 ℃, day temperature is controlled at 20 ℃～25 ℃, illumination is scattered light, the growth of group training seedling sprayed N:P:K:Ca:Mg mass ratio 23:1:21:4:1 one time after 7 days every 7 days, the nutrient solution that total concentration is 225mg/L is (containing N, P, K, the total concentration of Ca and Mg), the preparation of nutrient solution can be used ammonium nitrate, potassium dihydrogen phosphate, potassium chloride, calcium chloride, magnesium sulfate, mass ratio by described N:P:K:Ca:Mg is that 23:1:21:4:1 is formulated.
(2) water planting is no longer carried out water planting after three months, take out water container, allow the konjaku plant naturally fall seedling, leaf abscission, can gather in the crops average weight miniature kind of taro of ripe konjaku at 1.5 grams, miniature kind of taro maximum of konjaku reach 3 grams, the konjaku group training shoot survival percent of not taking root during the water planting breeding reaches 85%-90%, and every konjaku group training seedling survived all has kind of a taro, therefore, knot taro rate has also reached 85%-90%, has produced the technique effect of unforeseeable technique effect.
Adopt the inventive method can repeat easily 4 cycles in 1 year, can gather in the crops miniature kind of taro of 4 batches of konjakus, improved the coefficient of breeding of planting taro.
Claims (2)
1. the water planting breeding method of miniature kind of taro of a konjaku is characterized in that:
(1) the konjaku group of the not taking root training seedling cutting that the height of seedling of Plant Tissue Breeding being produced is 4cm～5cm, cutting is that the warty callus of the petiole of this seedling and its base portion is cut open, then after rinsing with running water the medium that removes group training seedling base portion, with sponge wrapping group training seedling base portion, replanting in the dish of cave uses running water in indoor water planting, group training seedling hydroponics growing is after 7 days, sprayed N:P:K:Ca:Mg mass ratio 23:1:21:4:1 one time every 7 days, the nutrient solution that total concentration is 225mg/L, during water planting in control room nocturnal temperature be 10 ℃～15 ℃, day temperature is 20 ℃～25 ℃, illumination is scattered light,
(2) water planting is no longer carried out water planting in 3 months, allows plant naturally fall seedling, can gather in the crops miniature kind of ripe taro.
2. the water planting breeding method of miniature kind of taro of konjaku according to claim 1, is characterized in that the plant square that described group of training seedling of step (1) implanted in the dish of cave is 5.2cm * 5.2cm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310407197.6A CN103430822B (en) | 2013-09-09 | 2013-09-09 | Aquaculture seed reproduction method for micro seed tubers of konjac |
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| CN201310407197.6A CN103430822B (en) | 2013-09-09 | 2013-09-09 | Aquaculture seed reproduction method for micro seed tubers of konjac |
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| CN103430822A true CN103430822A (en) | 2013-12-11 |
| CN103430822B CN103430822B (en) | 2015-03-18 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107211891A (en) * | 2017-05-27 | 2017-09-29 | 安康市农业科学研究所 | A kind of live fast breeding technique of konjaku callus |
| CN107318305A (en) * | 2017-08-18 | 2017-11-07 | 安康学院 | A kind of konjaku true seed fast breeding method |
| CN113973689A (en) * | 2021-11-29 | 2022-01-28 | 云南省热带作物科学研究所 | Matrix for rapidly breeding bulbil konjac small seed konjac and breeding method |
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| CN1295790A (en) * | 1999-11-14 | 2001-05-23 | 云南省农业科学院生物技术研究所 | Amorphophallus rivieri group seedling-culturing batch production and cultivation technology |
| CN1332957A (en) * | 2001-08-31 | 2002-01-30 | 中国科学院石家庄农业现代化研究所 | Industrialized method for quick culturing Amorphophallus rivieri |
| CN1422526A (en) * | 2002-12-10 | 2003-06-11 | 西南农业大学 | Test tube quick-breeding method for konjak |
| CN1493186A (en) * | 2003-08-09 | 2004-05-05 | 云南省农业科学院生物技术研究所 | Breeding technology of konjak testtube taro |
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2013
- 2013-09-09 CN CN201310407197.6A patent/CN103430822B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1295790A (en) * | 1999-11-14 | 2001-05-23 | 云南省农业科学院生物技术研究所 | Amorphophallus rivieri group seedling-culturing batch production and cultivation technology |
| CN1332957A (en) * | 2001-08-31 | 2002-01-30 | 中国科学院石家庄农业现代化研究所 | Industrialized method for quick culturing Amorphophallus rivieri |
| CN1422526A (en) * | 2002-12-10 | 2003-06-11 | 西南农业大学 | Test tube quick-breeding method for konjak |
| CN1493186A (en) * | 2003-08-09 | 2004-05-05 | 云南省农业科学院生物技术研究所 | Breeding technology of konjak testtube taro |
Non-Patent Citations (1)
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| 陈永波: "魔芋试管苗的营养液栽培管理技术和立体栽培模式初探", 《湖北民族学院学报(自然科学版)》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107211891A (en) * | 2017-05-27 | 2017-09-29 | 安康市农业科学研究所 | A kind of live fast breeding technique of konjaku callus |
| CN107318305A (en) * | 2017-08-18 | 2017-11-07 | 安康学院 | A kind of konjaku true seed fast breeding method |
| CN107318305B (en) * | 2017-08-18 | 2020-12-25 | 安康学院 | Rapid breeding method for konjac seedling seeds |
| CN113973689A (en) * | 2021-11-29 | 2022-01-28 | 云南省热带作物科学研究所 | Matrix for rapidly breeding bulbil konjac small seed konjac and breeding method |
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| CN103430822B (en) | 2015-03-18 |
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