CN107318305B - Rapid breeding method for konjac seedling seeds - Google Patents

Rapid breeding method for konjac seedling seeds Download PDF

Info

Publication number
CN107318305B
CN107318305B CN201710713728.2A CN201710713728A CN107318305B CN 107318305 B CN107318305 B CN 107318305B CN 201710713728 A CN201710713728 A CN 201710713728A CN 107318305 B CN107318305 B CN 107318305B
Authority
CN
China
Prior art keywords
seeds
value
standard solution
seedling
culture tank
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201710713728.2A
Other languages
Chinese (zh)
Other versions
CN107318305A (en
Inventor
李川
崔鸣
王健
王瑞娴
何斐
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ankang University
Original Assignee
Ankang University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ankang University filed Critical Ankang University
Priority to CN201710713728.2A priority Critical patent/CN107318305B/en
Publication of CN107318305A publication Critical patent/CN107318305A/en
Application granted granted Critical
Publication of CN107318305B publication Critical patent/CN107318305B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics

Abstract

The invention discloses a rapid breeding method of konjak seedling seeds, which comprises the following steps: (1) selecting konjak seedling seeds; (2) carrying out laminated freezing treatment on seeds; (3) carrying out high-temperature treatment on the seeds for 24 hours; (4) soaking the seeds, then covering the seeds for moisture preservation, and transplanting the seeds into a water culture tank after the seeds sprout; (5) preparing nutrient solution according to 1/5 concentration of the garden type standard formula solution for later use; (6) placing the prepared nutrient solution into a water culture tank, transplanting germinated seeds into the water culture tank, and fixing on a planting plate; (7) checking the EC value and the pH value of the nutrient solution in the water culture tank every 2 to 6 days, and adjusting to ensure that the EC value and the pH value are in proper ranges; (8) controlling the outside temperature of the water culture tank within the range of 25-35 ℃, and controlling the humidity within the range of 60-70 ℃; (9) after 90 days, the konjac seeds are bred into small corms, taken out, aired and stored to be used as seed taros for sowing. The invention can rapidly breed the konjak seeds.

Description

Rapid breeding method for konjac seedling seeds
Technical Field
The invention relates to the technical field related to seed cultivation, in particular to a rapid breeding method for konjac seedling seeds.
Background
The seed of konjak is the sexual organ of konjak obtained by natural or artificial pollination after the konjak blooms. The growth process of the konjak seedling seeds is the process that the konjak seeds are dormant for a long time after being mature, germinate and emerge, and finally form small bulbs.
The propagation mode of konjak mainly adopts the bulb and the rhizome of konjak and other asexual propagation modes. The main problems are that the konjac adopts asexual propagation for a long time, the seed source is single, the disease of the progeny is easy to accumulate, the degeneration is very serious, and the yield and the quality of the konjac are directly influenced. The seed propagation of the konjak seedlings can not only give play to heterosis, enhance disease resistance and improve propagation coefficient, but also prevent the konjak seeds from degeneration, which is an effective way for solving the virus-free propagation of the konjak seeds. However, because the dormancy period of the konjak seedling seeds is long under natural conditions, the emergence rate is extremely low (the emergence rate of farmer sowing is lower than 30%), and the konjak seedling seeds are difficult to be directly applied in production. The invention utilizes modern facility gardening and soilless culture technology, and aims to increase the source of the konjac seeds, improve the emergence rate of the seedling seeds, accelerate the breeding process of the konjac seeds, enhance the resistance of the konjac seeds and further improve the yield and the quality of the konjac seeds.
For example: the application number CN201210364297.0, entitled "a method for rapidly propagating No. 1 Delhi seed taro" provides the steps of seedling seed cultivation, seed harvesting and preservation, seedling substrate preparation, plug seedling, field transplantation, field management, harvesting and storage, and the seed taro is rapidly propagated by utilizing No. 1 Delhi seed, the propagation multiple of the seed taro is up to 600 times, and is more than 150 times of that of conventional block-cutting propagation. The method solves the problems of low propagation coefficient, serious virus accumulation and fast degeneration of the seed konjac in the propagation of the seed konjac, and has the advantages of simple operation method, good seed konjac quality and low cost. The defect is that the breeding method belongs to the traditional seedling raising method and cannot ensure higher seedling rate. And (II) the sowing time is limited, and the method is similar to a field propagation method. And (III) breeding new taro seeds by using the seedling seeds can be omitted greatly due to small harvest of the taro seeds.
For another example, the application number is CN201310407197.6, the invention name is 'a water culture seed reproduction method of konjak micro seed taro', and the proposed method is that a non-rooted konjak tissue culture seedling with the height of 4-5cm is cut off by a knife from a leaf stem and a tumor-shaped callus of a base part of the seedling, the tissue culture seedling is washed by water and then wrapped by a sponge at the base part of the tissue culture seedling, the tissue culture seedling is planted in a plug tray and cultivated by tap water indoors in a water culture way, and N is sprayed every 7 days after the tissue culture seedling grows for 7 days: p: k: ca: the mass ratio of Mg is 23: 1: 21:4: 1, nutrient solution with the total concentration of 225mg/L, wherein the indoor night is 10-15 ℃, the daytime is 20-25 ℃, and the illumination is scattered light; after 3 months of water culture, water culture is not carried out any more, and the plants are naturally transplanted to obtain mature micro seed taro. The method can breed 1.5 g of micro seed konjac on average in three months, greatly shortens the propagation period of high-quality seed konjac, and has low production cost, survival rate and konjac bearing rate of 85-90%. The invention has the defects that the average 1.5 g of the konjak micro seed taro can be bred in three months in the method (I), but the period required by the early-stage tissue culture from callus to bud differentiation to the height of 4-5cm of seedlings is longer, generally 3-6 months (II) are required, only the water culture mode of tissue culture seedlings is mentioned, and the seed growing mode is not mentioned. And (III) the cost is higher by adopting a tissue culture mode.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a rapid breeding method of konjak seedling seeds, which utilizes modern facility gardening and soilless culture technology to improve the yield of the seedling seeds, accelerate the process of breeding konjak seed taro by the konjak seedling seeds and provide a technical method with strong operability for producing the high-quality and high-disease-resistant seed taro.
In order to achieve the technical purpose, the invention adopts the following technical scheme:
a rapid breeding method for konjac seedling seeds comprises the following steps:
(1) selecting konjak seedling seeds;
(2) carrying out laminated freezing treatment on seeds;
(3) carrying out high-temperature treatment on the seeds for 24 hours;
(4) soaking the seeds, then covering the seeds for moisture preservation, and transplanting the seeds into a water culture tank after the seeds sprout;
(5) preparing nutrient solution according to 1/5 concentration of the garden type standard formula solution for later use;
(6) placing the prepared nutrient solution into a water culture tank, transplanting germinated seeds into the water culture tank, and fixing on a planting plate;
(7) checking the EC value and the pH value of the nutrient solution in the water culture tank every 2 to 6 days, and adjusting to ensure that the EC value and the pH value are in proper ranges;
(8) controlling the outside temperature of the water culture tank within the range of 25-35 ℃, and controlling the humidity within the range of 60-70 ℃;
(9) after 90 days, the konjac seeds are bred into small corms, taken out, aired and stored to be used as seed taros for sowing.
In the step (1), when selecting the konjac seedling seeds, selecting mature berry fruits with high artificial pollination seed setting rate, drying in the sun, removing peel, taking out the seedling seeds, and screening the seedling seeds with the grain weight of more than or equal to 0.2g for later use.
In the step (2), when the seeds are subjected to laminated freezing treatment, the seeds and wet sand with the water content of 10-15% are mixed according to the proportion of 1:3, are flatly laid in a tray, and are placed in a refrigerator with the temperature of 2-7 ℃ for 10-15 days.
Preferably, the temperature in the refrigerator is set to 5 ℃.
In the step (3), when the seeds are subjected to high-temperature treatment, the seeds are preferably screened out and then baked in an oven at 30 ℃ for 24 hours.
In step (4), the seeds were wrapped with a wet cloth, placed in a container, and treated with 5mg/L GA3And soaking the seeds for 24 hours, covering and moisturizing, putting the seeds in a cloth bag at the temperature of 28-30 ℃ for accelerating germination, opening the bag every day, washing the seeds with clear water, and transplanting the seeds into a water culture tank when the seeds germinate.
And (6) wrapping the bud tips of the konjak seedling seeds upwards by using polyurethane sponge, clamping non-woven fabric strips in the middle, and fixing the konjak seedling seeds on a planting plate in a water planting tank.
In step (7), the EC value adjustment rule is: the EC value of the sowing period is controlled to be 1.0ms/cm, the EC value of the seedling emergence rooting period is controlled to be 1.2ms/cm, the EC value of the head changing period is controlled to be 1.5ms/cm, and the EC value of the expansion period is controlled to be 2 ms/cm; the pH value is controlled to be 6.0-6.5.
The pH value can be adjusted and controlled by dilute phosphoric acid or potassium hydroxide.
In the step (8), the outside temperature of the hydroponic tank is preferably controlled to be 27 ℃ and the humidity is preferably controlled to be 65 ℃.
By adopting the technical scheme, the method has the following beneficial effects:
(1) shortening the breeding time of the seed taro. Using the method of the present invention, bulbs can be cultivated in about 90 days. However, the rhizome of the common konjac seed in the field needs to be used as a propagation material, and the bulb can be obtained after the seed is planted for one year. Moreover, the method can also fully utilize the seedling seeds harvested in 7 months to breed the taro seeds in 9-12 months, thereby greatly shortening the breeding time of the taro seeds and saving more than 1 year compared with the traditional breeding mode.
(2) High yield of the grown taro, high quality of the planted taro, strong resistance and high economic benefit. The method has high yield, and the formation rate of the seed tuber can reach more than 95%. The seedling seeds are utilized for breeding, the defect of long-term asexual propagation is overcome, the resistance is strong, the konjac seeds are neat and consistent after the konjac seeds are cultivated by adopting facilities, and the high-quality disease-resistant konjac seeds are provided for the production of commercial konjac.
(3) Low cost and convenient management. The method reduces the risk of field seedling raising and avoids unnecessary waste of manpower, material resources and financial resources. The breeding process is carried out in facilities, the land utilization rate is high, the management is convenient, and the plant diseases and insect pests are not easy to occur.
(4) The industrial production is realized, and the standardization degree is high. The taro is bred in a centralized way, so that unified management, unified operation rules and unified technology are facilitated, the taro yield is improved, and the standardized breeding of the taro is realized.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
The invention is further illustrated by the following examples.
Example 1
The invention relates to a rapid breeding method of konjak seedling seeds, which comprises the following steps:
(1) adopting mature berry fruits with high artificial pollination maturing rate which are taken in the same year, removing peel after airing, taking out the seedling seeds, removing smaller seeds, and selecting large-grain seeds (grain weight is more than or equal to 0.2g) with full seeds, positive appearance, smooth epidermis, no damage, no disease spot and uniformity.
(2) The seeds were mixed with wet sand having a water content of 10% in a ratio of 1:3, spread in a tray, and then placed in a refrigerator at 2 ℃ for 10 days.
(3) Sieving out seeds, and baking in an oven at 30 deg.C for 24 hr.
(4) Wrapping the seeds with wet cloth, placing in a container, and adding 5mg/L GA3Soaking the seeds for 24h, covering and moisturizing, placing in a cloth bag at 28 ℃ for accelerating germination, opening every day, washing the seeds with clear water, and transplanting the seeds into a water culture tank when the seeds germinate.
The seeds are placed in a cloth bag for accelerating germination, and the washing of the seeds is opened every day so as to wash off mucus seeped out from the seeds during accelerating germination and prevent mildew and rot.
(5) The nutrient solution was then prepared for use according to the 1/5 concentration of the garden standard formula, as shown in the table below.
(6) And (3) placing the prepared nutrient solution into a water culture tank, wrapping the bud tips of the konjak seedling seeds with upward tips by polyurethane sponge, clamping a non-woven fabric cloth strip in the middle, and fixing the non-woven fabric cloth strip on a field planting plate in the water culture tank. The seeds cannot come into contact with the nutrient solution. The water tank with the height of 15cm is selected as the water culture tank, and the water culture tank is moderate in size, easy to manage and suitable for carrying. And (3) paving a foam planting plate with the thickness of 1.5cm on the box body, wherein the size of the foam planting plate is suitable for just covering the box body. The plate was perforated with an aperture of about 2.5cm and a row spacing of 5cm x 5 cm.
And (3) spraying and disinfecting the water tank and the field planting plate with 800 times of potassium permanganate before field planting. The water tank is filled with water, the field planting plate is covered in time, and the water level is required to have a space of 2cm from the field planting plate.
(7) Checking the EC value and the pH value of the nutrient solution in the water culture tank every 2 days, adjusting to ensure that the EC value and the pH value are in proper ranges, wherein the EC value adjusting rule is as follows: the EC value of the sowing period is controlled to be 1.0ms/cm, the EC value of the seedling emergence rooting period is controlled to be 1.2ms/cm, the EC value of the head changing period is controlled to be 1.5ms/cm, and the EC value of the expansion period is controlled to be 2 ms/cm; the pH value is controlled between 6.0 and 6.5, and the pH value can be adjusted and controlled by dilute phosphoric acid or potassium hydroxide.
(8) The outside temperature of the water culture tank is controlled to be 25 ℃, and the humidity is controlled to be 60 ℃.
(9) After 90 days, the konjac seeds are bred into small corms, taken out, aired and stored to be used as seed taros for sowing.
Wherein the thickness of the layer is not more than 30cm when the seeds are subjected to the layer-freezing treatment. And (4) tightly wrapping the bud tips of the konjak seedling seeds upwards by using polyurethane sponge in the step (6) to avoid the nutrient solution from leaking into the konjak seedling seeds.
In steps (7) and (8), attention is paid to the regular replacement of the nutrient solution. Avoid the pollution of nutrient solution.
In the step (9), the seed taro is harvested, and the seed coat left after the head is changed is removed.
Example 2
The invention relates to a rapid breeding method of konjak seedling seeds, which comprises the following steps:
(1) adopting mature berry fruits with high artificial pollination maturing rate which are taken in the same year, removing peel after airing, taking out the seedling seeds, removing smaller seeds, and selecting large-grain seeds (grain weight is more than or equal to 0.2g) with full seeds, positive appearance, smooth epidermis, no damage, no disease spot and uniformity.
(2) The seeds were mixed with wet sand having a water content of 15% in a ratio of 1:3, spread in a tray, and then placed in a refrigerator at 7 ℃ for 10 days.
(3) Sieving out seeds, and baking in an oven at 30 deg.C for 24 hr.
(4) Wrapping the seeds with wet cloth, placing in a container, and adding 5mg/L GA3Soaking the seeds for 24h, covering and moisturizing, placing in a cloth bag at 30 ℃ for accelerating germination, opening every day, washing the seeds with clear water, and transplanting the seeds into a water culture tank when the seeds germinate.
The seeds are placed in a cloth bag for accelerating germination, and the washing of the seeds is opened every day so as to wash off mucus seeped out from the seeds during accelerating germination and prevent mildew and rot.
(5) The nutrient solution was then prepared according to the 1/5 concentration of the garden standard formula for future use.
(6) And (3) placing the prepared nutrient solution into a water culture tank, wrapping the bud tips of the konjak seedling seeds with upward tips by polyurethane sponge, clamping a non-woven fabric cloth strip in the middle, and fixing the non-woven fabric cloth strip on a field planting plate in the water culture tank. The seeds cannot come into contact with the nutrient solution. The water tank with the height of 15cm is selected, the size is moderate, the management is easy, and the transportation is suitable. And (3) paving a foam planting plate with the thickness of 1.5cm on the box body, wherein the size of the foam planting plate is suitable for just covering the box body. The plate was perforated with an aperture of about 2.5cm and a row spacing of 5cm x 5 cm.
And (3) spraying and disinfecting the water tank and the field planting plate with 800 times of potassium permanganate before field planting. The water tank is filled with water, the field planting plate is covered in time, and the water level is required to have a space of 2cm from the field planting plate.
(7) Checking the EC value and the pH value of the nutrient solution in the water culture tank every 6 days, adjusting to ensure that the EC value and the pH value are in proper ranges, wherein the EC value adjusting rule is as follows: the EC value of the sowing period is controlled to be 1.0ms/cm, the EC value of the seedling emergence rooting period is controlled to be 1.2ms/cm, the EC value of the head changing period is controlled to be 1.5ms/cm, and the EC value of the expansion period is controlled to be 2 ms/cm; the pH value is controlled between 6.0 and 6.5, and the pH value can be adjusted and controlled by dilute phosphoric acid or potassium hydroxide.
(8) The outside temperature of the water culture tank is controlled to be 35 ℃, and the humidity is controlled to be 70 ℃.
(9) After 90 days, the konjac seeds are bred into small corms, taken out, aired and stored to be used as seed taros for sowing.
Wherein the thickness of the layer is not more than 30cm when the seeds are subjected to the layer-freezing treatment. And (4) tightly wrapping the bud tips of the konjak seedling seeds upwards by using polyurethane sponge in the step (6) to avoid the nutrient solution from leaking into the konjak seedling seeds.
In steps (7) and (8), attention is paid to the regular replacement of the nutrient solution. Avoid the pollution of nutrient solution.
In the step (9), the seed taro is harvested, and the seed coat left after the head is changed is removed.
Example 3
The invention relates to a rapid breeding method of konjak seedling seeds, which comprises the following steps:
(1) adopting mature berry fruits with high artificial pollination maturing rate which are taken in the same year, removing peel after airing, taking out the seedling seeds, removing smaller seeds, and selecting large-grain seeds (grain weight is more than or equal to 0.2g) with full seeds, positive appearance, smooth epidermis, no damage, no disease spot and uniformity.
(2) The seeds were mixed with wet sand having a water content of 13% in a ratio of 1:3, spread in a tray, and then placed in a refrigerator at 5 ℃ for 10 days.
(3) Sieving out seeds, and baking in an oven at 30 deg.C for 24 hr.
(4) Wrapping the seeds with wet cloth, placing in a container, and adding 5mg/L GA3Soaking seeds for 24 hr, covering, moistening, placing in cloth bag at 29 deg.C for accelerating germination, opening every day, and adding clear waterWashing the seeds, and transplanting the seeds into the water culture tank after the seed buds appear.
The seeds are placed in a cloth bag for accelerating germination, and the washing of the seeds is opened every day so as to wash off mucus seeped out from the seeds during accelerating germination and prevent mildew and rot.
(5) The nutrient solution was then prepared according to the 1/5 concentration of the garden standard formula for future use.
(6) And (3) placing the prepared nutrient solution into a water culture tank, wrapping the bud tips of the konjak seedling seeds with upward tips by polyurethane sponge, clamping a non-woven fabric cloth strip in the middle, and fixing the non-woven fabric cloth strip on a field planting plate in the water culture tank. The seeds cannot come into contact with the nutrient solution. The water tank with the height of 15cm is selected, the size is moderate, the management is easy, and the transportation is suitable. And (3) paving a foam planting plate with the thickness of 1.5cm on the box body, wherein the size of the foam planting plate is suitable for just covering the box body. The plate was perforated with an aperture of about 2.5cm and a row spacing of 5cm x 5 cm.
And (3) spraying and disinfecting the water tank and the field planting plate with 800 times of potassium permanganate before field planting. The water tank is filled with water, the field planting plate is covered in time, and the water level is required to have a space of 2cm from the field planting plate.
(7) Checking the EC value and the pH value of the nutrient solution in the water culture tank every 2 days, adjusting to ensure that the EC value and the pH value are in proper ranges, wherein the EC value adjusting rule is as follows: the EC value of the sowing period is controlled to be 1.0ms/cm, the EC value of the seedling emergence rooting period is controlled to be 1.2ms/cm, the EC value of the head changing period is controlled to be 1.5ms/cm, and the EC value of the expansion period is controlled to be 2 ms/cm; the pH value is controlled between 6.0 and 6.5, and the pH value can be adjusted and controlled by dilute phosphoric acid or potassium hydroxide.
(8) The outside temperature of the water culture tank is controlled to be 27 ℃, and the humidity is controlled to be 65 ℃.
(9) After 90 days, the konjac seeds are bred into small corms, taken out, aired and stored to be used as seed taros for sowing.
Wherein the thickness of the layer is not more than 30cm when the seeds are subjected to the layer-freezing treatment. And (4) tightly wrapping the bud tips of the konjak seedling seeds upwards by using polyurethane sponge in the step (6) to avoid the nutrient solution from leaking into the konjak seedling seeds.
In steps (7) and (8), attention is paid to the regular replacement of the nutrient solution. Avoid the pollution of nutrient solution.
In the step (9), the seed taro is harvested, and the seed coat left after the head is changed is removed.
Example 4
This example differs from the previous examples in that the current year ripe berry fruit (amorphophallus konjac fruit changed to orange) was harvested in 8 months, the peel and testa were removed after air-curing, the seedling seeds were removed, the seed size was classified, and seeds with a grain weight of more than 0.2g were selected
And then mixing and flatly paving the seeds and the wet sand in a ratio of 1:3 in a tray at the beginning of 9 months, wherein the height of the tray is about 10cm, and then placing the tray in a refrigerator at the temperature of 5 ℃ for 10 to 15 days.
Taking out the cultured konjac corms at the beginning of 12 months, airing, storing at a low temperature of about 5 ℃, and directly sowing as seed konjac at 4 months in the next year.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and additions can be made without departing from the principle of the present invention, and these should also be considered as the protection scope of the present invention.

Claims (5)

1. A rapid propagation method of konjak seedling seeds is characterized by comprising the following steps:
(1) selecting konjak seedling seeds, selecting mature berry fruits with high artificial pollination seed setting rate when selecting the konjak seedling seeds, removing peel after drying in the sun, taking out the seedling seeds, and screening the seedling seeds with the grain weight of more than or equal to 0.2g for later use;
(2) performing laminated freezing treatment on the seeds, mixing the seeds with wet sand with the water content of 10-15% according to the proportion of 1:3, flatly paving the mixture in a tray, and then placing the tray in a refrigerator at the temperature of 2-7 ℃ for 10-15 days;
(3) performing high-temperature treatment on the seeds for 24 hours, screening the seeds out and then baking the seeds in an oven at the temperature of 30 ℃ for 24 hours;
(4) soaking the seeds, then covering and moisturizing, transplanting the seeds into a water culture tank after the seeds sprout, wrapping the seeds with wet cloth, putting the seeds into a container, soaking the seeds for 24 hours with 5mg/L GA3, covering and moisturizing, putting the seeds into a cloth bag at 28-30 ℃ for accelerating germination, opening the bag every day and washing the seeds with clear water, and transplanting the seeds into the water culture tank after the seeds sprout;
(5) preparing nutrient solution according to 1/5 concentration of garden type standard formula solution prepared from Ca (NO)3)2·4H2O (945 mg/L of standard solution; 189mg/L of 1/5 standard solution) and KNO3(809 mg/L of standard solution; 161.8mg/L of 1/5 standard solution), NH4H2PO4(153 mg/L of standard solution; 1/5 of standard solution, 30.6mg/L), MgSO4·7H2O (493 mg/L of standard solution; 1/5 98.6mg/L of standard solution), Na2Fe-EDTA (standard solution 20 mg/L; standard solution 1/5 concentration 4.0mg/L), MnSO4·4H2O (standard solution 2.13 mg/L; standard solution 1/5 concentration 0.426mg/L), H3BO3(standard solution 2.86 mg/L; standard solution 1/5 concentration 0.572mg/L), ZnSO4·7H2O (standard solution 0.22 mg/L; 1/5 standard solution concentration 0.044mg/L), CuSO4·5H2O (standard solution 0.08 mg/L; 1/5 standard solution concentration 0.016mg/L), (NH)4)6Mo7O24·4H2O (standard solution 0.02 mg/L; 1/5 standard solution 0.004 mg/L);
(6) placing the prepared nutrient solution into a water culture tank, transplanting germinated seeds into the water culture tank, and fixing on a planting plate;
(7) checking the EC value and the pH value of the nutrient solution in the water culture tank every 2 to 6 days, adjusting to ensure that the EC value and the pH value are in proper ranges, wherein the EC value adjusting rule is as follows: the EC value of the sowing period is controlled to be 1.0ms/cm, the EC value of the seedling emergence rooting period is controlled to be 1.2ms/cm, the EC value of the head changing period is controlled to be 1.5ms/cm, and the EC value of the expansion period is controlled to be 2 ms/cm; controlling the pH value to be 6.0-6.5;
(8) controlling the outside temperature of the water culture tank within the range of 25-35 ℃, and controlling the humidity within the range of 60-70 ℃;
(9) after 90 days, the konjac seeds are bred into small corms, taken out, aired and stored to be used as seed taros for sowing.
2. The method for rapid propagation of the seedling seeds of amorphophallus konjac as claimed in claim 1, wherein: the temperature in the refrigerator was set at 5 ℃.
3. The method for rapid propagation of the seedling seeds of amorphophallus konjac as claimed in claim 1, wherein: and (6) wrapping the bud tips of the konjak seedling seeds upwards by using polyurethane sponge, clamping non-woven fabric strips in the middle, and fixing the konjak seedling seeds on a planting plate in a water planting tank.
4. The method for rapid propagation of the seedling seeds of amorphophallus konjac as claimed in claim 1, wherein: the pH value is adjusted and controlled by dilute phosphoric acid or potassium hydroxide.
5. The method for rapid propagation of the seedling seeds of amorphophallus konjac as claimed in claim 1, wherein: in the step (8), the outside temperature of the water culture tank is controlled to be 27 ℃, and the humidity is controlled to be 65 ℃.
CN201710713728.2A 2017-08-18 2017-08-18 Rapid breeding method for konjac seedling seeds Expired - Fee Related CN107318305B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710713728.2A CN107318305B (en) 2017-08-18 2017-08-18 Rapid breeding method for konjac seedling seeds

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710713728.2A CN107318305B (en) 2017-08-18 2017-08-18 Rapid breeding method for konjac seedling seeds

Publications (2)

Publication Number Publication Date
CN107318305A CN107318305A (en) 2017-11-07
CN107318305B true CN107318305B (en) 2020-12-25

Family

ID=60228090

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710713728.2A Expired - Fee Related CN107318305B (en) 2017-08-18 2017-08-18 Rapid breeding method for konjac seedling seeds

Country Status (1)

Country Link
CN (1) CN107318305B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108848742B (en) * 2018-06-25 2021-05-04 云南大学 Low-temperature germination accelerating treatment method for konjak bulbs
CN109429961B (en) * 2018-10-24 2022-03-18 云南大学 Rapid breeding method for konjac fruiting

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1295790A (en) * 1999-11-14 2001-05-23 云南省农业科学院生物技术研究所 Amorphophallus rivieri group seedling-culturing batch production and cultivation technology
CN1528114A (en) * 2003-09-28 2004-09-15 东莞市林业科学研究院 Konjak in vitro rapid breeding method
CN1775002A (en) * 2005-11-21 2006-05-24 云南耕耘魔芋资源开发有限责任公司 Bulbil konjak artificial induced flowering asexual breeding method
CN101543184A (en) * 2008-03-29 2009-09-30 恩施土家族苗族自治州农业科学院 Method for culturing open type tissue of konjak
CN102763597A (en) * 2012-08-15 2012-11-07 云南省农业科学院生物技术与种质资源研究所 Two-generation in one year reproduction method for konjac cross breeding
CN103125238A (en) * 2013-02-27 2013-06-05 昆明学院 Multi-seedling autogenic relay growth method for seeds of amorphophallus muelleri
CN103430822A (en) * 2013-09-09 2013-12-11 云南省农业科学院生物技术与种质资源研究所 Aquaculture seed reproduction method for micro seed tubers of konjac
CN103798118A (en) * 2014-02-27 2014-05-21 北京农学院 Experimental leaf vegetable water culture device
CN105340420A (en) * 2015-11-25 2016-02-24 临沧滇晟农林发展有限公司 Method for catalyzing germination of seedling seeds of amorphophallus bulbifer
CN106717247A (en) * 2016-11-21 2017-05-31 云南民族大学 A kind of cultural method of true seed high yield blade face bulb of being bloomed by bulbil type konjac

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105432301B (en) * 2015-12-07 2018-10-26 竹溪县佳兴魔芋食品有限公司 A method of improving konjaku vegetative propagation rate
CN106385860A (en) * 2016-08-31 2017-02-15 怒江滇鑫科技有限责任公司 Method for processing konjak seeds

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1295790A (en) * 1999-11-14 2001-05-23 云南省农业科学院生物技术研究所 Amorphophallus rivieri group seedling-culturing batch production and cultivation technology
CN1528114A (en) * 2003-09-28 2004-09-15 东莞市林业科学研究院 Konjak in vitro rapid breeding method
CN1775002A (en) * 2005-11-21 2006-05-24 云南耕耘魔芋资源开发有限责任公司 Bulbil konjak artificial induced flowering asexual breeding method
CN101543184A (en) * 2008-03-29 2009-09-30 恩施土家族苗族自治州农业科学院 Method for culturing open type tissue of konjak
CN102763597A (en) * 2012-08-15 2012-11-07 云南省农业科学院生物技术与种质资源研究所 Two-generation in one year reproduction method for konjac cross breeding
CN103125238A (en) * 2013-02-27 2013-06-05 昆明学院 Multi-seedling autogenic relay growth method for seeds of amorphophallus muelleri
CN103430822A (en) * 2013-09-09 2013-12-11 云南省农业科学院生物技术与种质资源研究所 Aquaculture seed reproduction method for micro seed tubers of konjac
CN103798118A (en) * 2014-02-27 2014-05-21 北京农学院 Experimental leaf vegetable water culture device
CN105340420A (en) * 2015-11-25 2016-02-24 临沧滇晟农林发展有限公司 Method for catalyzing germination of seedling seeds of amorphophallus bulbifer
CN106717247A (en) * 2016-11-21 2017-05-31 云南民族大学 A kind of cultural method of true seed high yield blade face bulb of being bloomed by bulbil type konjac

Also Published As

Publication number Publication date
CN107318305A (en) 2017-11-07

Similar Documents

Publication Publication Date Title
CN102119660B (en) Method for rooting culture,seedling adaptation and transplantation of alpine rose tissue-cultured seedling in greenhouse
CN105746224B (en) A kind of kalopanax septemlobus seed is nursed young plants in hothouses method
CN107996166B (en) Rapid cuttage breeding method for catalpa bungei
CN104206243B (en) A kind of method improving radish side shoot root survival and breeding quality
CN105660284A (en) Method for producing red kiwi fruit seedlings
CN110235710A (en) A kind of plant factor's cultural method of three-coloured amaranth
CN106900326A (en) A kind of fast breeding, the implantation methods of high yield strawberry
CN107318305B (en) Rapid breeding method for konjac seedling seeds
CN110463453B (en) Cultivation method for breeding virus-free potato small seed by using aerial fog method
CN110338048B (en) Soilless culture method for cherry tomatoes
CN102150540A (en) Tobacco plug seedling method by combining artificial light with natural light
CN105766619B (en) A kind of breeding method of day lily seed
CN109526743B (en) Rapid propagation and fine variety breeding method for garlic callus
CN109315252A (en) A kind of Winter potato method for culturing and transplanting seedlings
CN109511534B (en) Berry seed sowing and breeding method
CN111742729A (en) Method for rapid propagation of high-quality tomato seedlings
CN111587746A (en) Method for cultivating small-sized watermelons in early spring
CN101243759B (en) Trunk-burying germination-forcing epicormic branch cuttage raise seedling technique
CN103053347B (en) Winter greenhouse grafting method for English hawthorn 'Scarlet' planted in Beijing area
CN107372070B (en) Hardwood cutting seedling raising method based on Typha river fragrant oranges
CN110214563A (en) It is a kind of to promote Crassulaceae succulent leaf to insert rooting method using IBA processing
CN110622716A (en) Tomato grafting and seedling raising method
CN110367017A (en) A kind of seed selection breeding method of white peach
CN105265193B (en) Cauliflower grafting is reserved seed for planting method
CN111788977B (en) Planting method for increasing survival rate of cryptomeria fortunei seedlings

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20201225

Termination date: 20210818

CF01 Termination of patent right due to non-payment of annual fee