CN113331013B - A kind of sowing and promoting cultivation method of pine lily - Google Patents

Abstract

A method for sowing and forcing culture of lilium pinosum relates to the field of lilium pinosum planting. The invention relates to a method for sowing and forcing culture of lilium songaricum, which comprises the following steps: step one, harvesting and pre-treating lilium pinifolium seeds; step two, preparing the lily bulb at a low temperature before dry sand storage and storing the lily bulb at the low temperature; step three, accelerating germination of the lilium pinosum; step four, sowing and maintenance management of the lilium pinifolium in winter in a greenhouse; step five, the lily seedlings are manually dormant at low temperature; step six, releasing dormancy of the lilium pinosum and cultivating, maintaining and managing in summer; step seven, forcing culture of lilium pinifolium seed bulbs; step eight, open field cultivation of the lilium pinifolium and preparation of a planting bed; and step nine, managing the planted lilium pinifolium. The invention aims to provide a method for sowing and forcing culture of lilium pinosum, which is used for improving germination rate and direct seeding rate of lilium pinosum seeds, shortening low-temperature germination accelerating time and breeding period of lilium pinosum, reducing propagation cost of lilium pinosum and realizing large-scale seedling breeding of lilium pinosum.

Description

A kind of sowing and promoting cultivation method of pine lily

technical field

The invention relates to the technical field of planting lily pine leaves, in particular to a method for sowing and promoting cultivation of lily pine leaves.

Background technique

Lilium cernuum, also known as Lilium cernuum, is a curly petal group in the plant classification of the genus Lilium, and it is also one of the most important wild species in Asian hybrid lily breeding. Lilium cernuum is mainly distributed in Northeast China (Jilin Province, Liaoning Province), Korean Peninsula, Russia and other places, among which the Changbai Mountains (China, North Korea) are the main distribution areas. Pine leaf lily (Lilium cernuum) is the only purple-red wild lily with fragrance in Northeast China.

At present, the propagation of Lilium cernuum (Lilium cernuum) can be carried out by sowing, cutting, tissue culture and other techniques, but the cutting and tissue culture techniques have the disadvantages of small number of seedlings and high cost, while the propagation by seed sowing has the disadvantages of low cost. , the advantages of a large amount of seedlings, but the emergence rate of lily pine leaves by seed sowing is low, and the difficulty of later maintenance and management will easily lead to low seedling rate. At the same time, lily pine leaves are monocotyledonous plants. Only one leaf grows in a year, and it can bloom from the 4th year, and the seedlings need at least 4 years to bloom.

Xu Ying et al. investigated the distribution, habitat, associated plants, population status and morphological characteristics of the Lily of Lily in Liaoning Province, and tested the effects of different substrates, GA3 concentrations and storage years on its seed germination. The results show that the distribution area of Lilium pendentis in Liaoning Province includes Fengcheng City, Fushun City, Benxi City and Kaiyuan City. It grows in grass, shrub forest or semi-shady broad-leaved forest, with rich associated plants and a narrow distribution range of resources. , the number of wild groups and individuals is greatly reduced than before. The underground bulb of Lily pendulum is long oval, the leaves are linear, the flowers are lavender, drooping and revolving, fragrant, the capsule is oblong, and the seeds are obovate and translucent. The suitable substrate for seed germination is river sand, with a germination rate of 72.0%; 100-200mg/L GA3 can promote seed germination to a certain extent, and the germination rate reaches 80.0%; mature seeds harvested in the same year and seeds stored at 4°C for one year The germination rate of sowing is relatively high, both reaching more than 80.0% (Xu Ying et al., Lilium pendulum resources survey and seed propagation research, seeds, 2011.8.25). In the above technical scheme, the seeds of Lilium pine leaves are sown after being stored at 4° C. for one year, and the germination rate reaches more than 80.0%, which has the disadvantage of long time for seed germination at low temperature.

Hu Pengqing et al. conducted a study on the seed characteristics and germination ability of wild Lilium chinensis seeds collected in Linjiang City, Jilin Province. The results showed that the seeds of Lilium pendantii had no dormancy characteristics, and began to germinate on the 5th day, and the germination rate reached 72.1%. The seedlings were transplanted into plug trays of two different matrices of 1 peat: 1 vermiculite and 2 peat: 1 perlite, and the survival rate reached over 90%. (Hu Pengqing et al. Seed germination test of Lilium pendulatum, Agricultural Science and Technology and Information, 2015.4.25). In the above technical scheme, the seeds of Lilium pine leaves do not have dormancy characteristics, the seedling emergence rate of direct seeding reaches 72.1%, and the survival rate of transplanting reaches more than 90%, which has the disadvantage of low seedling rate.

Chen Shaopeng et al. conducted research on the introduction and breeding techniques of wild pendant lily in the Changbai Mountain area. Specifically: the best induction medium for Lilium pine leaves is MS basic medium, and the hormone combination is 6-BA 1.0mg/L+NAA 0.3mg/L; the proliferation medium is MS basic medium with 30g sucrose added, and the hormone is 6-BA -BA 1.0mg/L and NAA 0.1 mg/L, the subculture time is 30 days; the addition of NAA 0.5mg/L, IBA 0.5 mg/L and 20g/L sucrose in 1/2MS medium can promote root healthy growth. The wild pendant lily seedlings after rooting are practiced and transplanted after 2 days. It grows best in the substrate of peat:garden soil:river sand=2:1:1. After transplanting, the survival rate of Lilium pine leaf seedlings was 79.0% (Chen Shaopeng et al., Research on the introduction and breeding technology of wild lily in Changbai Mountains, Jilin Forestry Research Institute, 2016.9.14).

Chen Shaopeng et al. studied the effects of different cutting conditions on the asexual reproduction of Lilium pine leaves. Specifically: the scales of Lilium pine leaves were used as materials, three kinds of substrates were used: river sand, perlite and peat soil, and two hormones of IAA and NAA at a concentration of 200mg· ^L Lily scale rooting rate, root length, new bulb quality, bulb diameter, bulb number and cotyledon length. The results showed that the IAA river sand substrate was the most suitable for cutting Lilium pine leaf scales, the rooting time was the shortest, the rooting rate reached 97%, the root length was 9.18cm, the weight of new bulbs was 0.13g, the bulb diameter was 0.62cm, the cotyledon length was 0.76cm, and the newborn 2.47 bulbs (Chen Shaopeng et al., Effects of different cutting conditions on the vegetative propagation of Lilium pine leaves, Agriculture and Technology, 2020.11.15). In the above-mentioned technical scheme, cutting and tissue culture are used for asexual propagation of Lilium pine leaves, the technology is complex and few plants are obtained, it is difficult to breed seedlings on a large scale, and the cost is high.

So far, there have been no reports on the post-sowing maintenance and cultivation methods of Lilium pine leaves.

Contents of the invention

The purpose of the present invention is to provide a kind of lily pine seeding and promoting cultivation method, to improve lily pine seed germination rate and direct seeding emergence rate, shorten lily pine germination time and breeding cycle at low temperature, reduce lily pine propagation cost, realize pine lily Leaf lily large-scale breeding seedlings.

The technical scheme that the present invention adopts for solving technical problems is as follows:

A kind of pine lily sowing and promoting cultivation method of the present invention mainly comprise the following steps:

Step 1. Harvesting and pre-treatment of Lilium pine leaves seeds;

Step 2. Preparation and low-temperature dry sand storage of Matsuba Lily before low-temperature dry sand storage;

Step 3, pine leaf lily germination;

Step 4, sowing and maintenance management of lily pine leaf in winter greenhouse;

Step 5, artificial low temperature dormancy of Lilium pine leaf seedlings;

Step 6. Lilium pine leaf dormancy release and summer cultivation and maintenance management;

Step 7, Lilium pine leaf bulbs promote cultivation;

Step 8, open field cultivation and planting bed preparation of lily pine leaves;

Step 9: Management of Lilium pine leaves after planting.

As a preferred embodiment, the specific operation process of step one is as follows:

Harvest in late September of the first year when the capsules are yellow and uncracked, and ventilate and dry indoors for 15-20 days. After the capsules are completely cracked, take out the seeds and remove immature seeds and impurities, and store the treated Lilium pine leaves seeds in a ziplock bag.

As a preferred embodiment, the specific operation process of step 2 is as follows:

Screen the dry river sand with a 1.0mm aperture soil sieve and clean it with running water, soak it in 0.5% potassium permanganate solution for 30 minutes, then rinse it with water for more than 3 times, and put the sterilized and cleaned fine sand in a metal plate Drying, control the water content below 1%;

Mix Lilium pine leaves seeds and fine sand evenly at a volume ratio of 1:3, put them in a ziplock bag or a sealed plastic box, and store them in a refrigerator at 4°C for 60 days to mid-December.

As a preferred embodiment, the specific operation process of step 3 is as follows:

Take out the seed sand mixture after sand storage for 60 days, soak it with 1.0% carbendazim solution or 1.0% chlorothalonil solution for 30min-1h, wash it with clean water for 2-3 times, and soak it in warm water at 30°C for 12-18h at room temperature until Until the white seed embryo in the middle of the Lilium pine leaves can be clearly observed, pour out the soaked water, put it in an artificial climate box, and use 25°C for 16h and 16°C for 8h for 5 days.

As a preferred embodiment, the specific operation process of step 4 is as follows:

(1) Lilium pine leaf sowing in winter greenhouse

Use a plastic seedling tray with drainage holes at the bottom to raise seedlings. The cultivation substrate is mixed with peat soil, perlite, and decomposed deer manure at a volume ratio of 3:1:0.5; put a part of the cultivation substrate in the plastic seedling tray and press After the temperature-changing treatment, the pine lily seeds and fine sand were evenly sprinkled and compacted, so that the pine lily seeds were in full contact with the cultivation medium; the remaining cultivation medium was used to fill the plastic seedling tray and compacted; use hymexazol 3000 Double liquid is poured slowly to sterilize;

(2) Greenhouse maintenance and management of pine leaf lily in winter

After sowing, move the plastic seedling trays into the winter greenhouse. The nighttime temperature of the greenhouse is controlled at 10-15°C, and the daytime temperature is controlled at 20-25°C; Spray once in 7-10 days with 3000 times hymexazol.

As a preferred embodiment, the specific operation process of step five is as follows:

In mid-April of the second year, dig out the seedlings of Lilium pine leaves together with the lower bulbs, remove the upper cotyledons and lower roots, dry them in a cool place for 1 day, and refrigerate them at 4°C to promote dormancy of Lily pine leaves. Dormancy for 60 days to mid-June.

As a preferred embodiment, the specific operation process of step 6 is as follows:

In mid-June, sow the lily bulbs after low temperature dormancy according to step 4 to relieve dormancy; place plastic seedling trays on an outdoor flat seedbed, set up a shed on the seedbed, and use a sunshade with a shading rate of 75-95% on the shed Shade and cover with nets, artificial weed control, keep the cultivation substrate in the plastic seedling tray moist but not waterlogged; Lilium pine leaves are cultivated and managed until mid-October in summer, after which Lilium pine leaves stop growing.

As a preferred embodiment, the specific operation process of step 7 is as follows:

In mid-October, dig out the pine lily bulbs in the plastic seedling tray, and perform the second artificial low-temperature dormancy according to step 5, and dormancy for 60 days until mid-December, and follow step 4 for planting and maintenance until the third year 4 In the middle of the month, finally carry out the third artificial low temperature dormancy according to step five.

As a preferred embodiment, the specific operation process of step eight is as follows:

Choose a higher terrain as the planting site, remove gravel and weeds from the soil of the open-field cultivation seedbed of pine lily before planting, and evenly spread 3.0g of chlorfluoroclothianidin per square meter of seedbed; before planting, use 0.5% Soak in carbendazim solution for 30min-1h, drain the water after soaking, and place in a cool and ventilated place to dry in the shade for 1-2h to ensure that there is no moisture on the surface of the pine lily bulbs; the pine lily bulbs are planted at a row spacing of 5.0cm×5.0cm , the planting depth is 8.0-10.0cm.

As a preferred embodiment, the specific operation process of step 9 is as follows:

After the lily of the pine leaf is planted in the open field, it is not watered, and weeds are removed regularly; after the above-ground part grows, sufficient light is ensured, and 1000 times of potassium dihydrogen phosphate is sprayed every 10-15 days, and the spraying is stopped until flowering in the same year.

The beneficial effects of the present invention are:

At present, some studies believe that the seeds of Lilium pine leaves do not have dormancy characteristics, so the seeds of Lilium pine leaves are directly broadcast after harvesting or stored at 4°C for 1 year. The emergence rate was 72.1%. In addition, Lilium pine leaf grows slowly, and the seeds stop growing after they mature in September each year, and enter a dormant state, and do not start to grow until mid-May of the following year, which causes Lily pine leaf to need at least 3 winter dormancy times from sowing to flowering. more than 4 years.

Aiming at the above-mentioned technical problems, the present invention adopts the methods of low-temperature dry sand storage of seeds, constant temperature soaking, artificial temperature-variable germination, winter greenhouse seedling management, winter greenhouse seedling management, and artificial promotion of dormancy, so that the germination rate of Lilium pine leaves can reach more than 90%. The emergence rate reaches more than 90%, which improves the germination rate and emergence rate of Lilium pine leaves. Simultaneously, the present invention makes the preservation rate of the lily of pine leaves reach more than 90% by means of shading maintenance and soil insecticide.

The staged low-temperature storage of pine lily bulbs adopted by the present invention and the winter cultivation method in the greenhouse break the dormancy of the bulbs, and at the same time promote cultivation to solve the problem that pine lily needs to be sown for more than 4 years before blooming. It takes 4 years to bloom, which can be reduced to 2 and a half years of cultivation, which greatly shortens the breeding cycle of Matsuba Lily and reduces the breeding cost of Matsuba Lily at the same time, using seeds to quickly obtain a large number of seedlings.

Description of drawings

Fig. 1 is a kind of pine leaf lily seeding of the present invention and the flow chart that promotes cultivation method.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

Example 1

1. Harvesting and pre-treatment of Lilium pine leaves seeds

The seeds of Lilium pine leaf were harvested on September 20, 2017, and they were harvested when the capsules were withered and yellow but not cracked. Bring the harvested capsules back to the room, and place them in a ventilated and dry place to dry for 15 days. After the capsules are completely cracked, take out the seeds in the capsules and place them on white paper, and blow them with a small electric fan of 5V and 3W to remove immature seeds. For impurities in seeds and capsules, store the treated Lilium pine leaves seeds in ziplock bags.

2. Preparation before low-temperature dry sand storage and low-temperature dry sand storage of Matsuba Lily

The dry river sand is screened with a soil sieve with a pore size of 1.0mm, and then fully washed with running water. Use 0.5% potassium permanganate solution to soak the fine sand for 30 minutes, then rinse the fine sand with clean water for 3 times, put the sterilized and cleaned fine sand in a metal plate, dry to remove excess water, and measure the water content of the fine sand with a hygrometer 0.8%.

3. Low temperature dry sand storage

On October 20, 2017, mix the Lily pine seeds that have been removed from the impurities and the fine sand that has been cleaned and dried according to the volume ratio of 1:3, put them in a ziplock bag or a sealed plastic box, and put them in a refrigerator at 4°C. 60 days to December 20.

4. Germination of pine leaf lily

Take out the seed sand mixture after sand storage for 60 days, soak it in 1.0% carbendazim solution for 30 minutes, wash it twice with clean water, put it in a plastic box, and soak it in warm water at 30°C for 12 hours at room temperature until the pine leaves can be clearly observed The white seed embryo in the middle of the lily seeds is poured out, and the soaked water is poured out, together with the plastic box, they are placed in an artificial climate box, and treated at 25°C for 16h and 16°C for 8h for 5 days.

After the variable temperature treatment was completed, 300 Lily pine seeds were selected in the sand mixture, divided into 3 groups, and 100 seeds in each group were tested for germination, which was used as the treatment group, and the untreated seeds stored in the room were used as the control group. Same as treatment group. Spread two layers of filter paper in the petri dish, make the filter paper full of water, place 25 seeds in each dish, place it in an incubator, keep the temperature stable at 25°C, replenish water in good time during the test, observe every day and count the germination rate at the same time For multiple comparisons, the test results are shown in Table 1. The results showed that the germination rate of Lilium pine leaves after treatment was significantly increased, reaching 92.67%, which was significantly different from the germination rate of the control group of 62.00%.

Table 1 Seed germination rate of Lilium pine leaves

group Germination rate(%) treatment group 92.67±3.64A control group 62.00±5.33B

Capital letters in the table represent the results of significant difference analysis, p≤0.01.

5. Sowing and maintenance management of Lilium pine leaf in winter greenhouse

(1) Lilium pine leaf sowing in winter greenhouse

The new seedlings of Lilium pine leaves are weak, so a plastic seedling tray with drainage holes at the bottom with a size of 54cm×27cm×6cm is used for seedling cultivation. Put a 4cm deep cultivation substrate into the plastic seedling tray and compact it, and then store 300 Lilium pine leaves seeds after 60 days of low-temperature sand storage, 12 hours of warm water soaking at 30°C, and 5 days of variable temperature treatment (100 seeds per group) as For the treatment group, select untreated seeds of the same number and grouping as the control group, sprinkle them evenly into plastic seedling trays together with fine sand and compact them, so that the seeds of Lilium pine leaves are in full contact with the lower cultivation substrate, and then fill the plastic with the remaining cultivation substrate. Seedling tray and compacted. The cultivation medium is mixed with peat soil, perlite, and decomposed deer manure in a volume ratio of 3:1:0.5. Use hymexazol 3000 times solution to slowly irrigate the control group and the treatment group to fully sterilize the soil and prevent damping-off.

(2) Greenhouse maintenance and management of pine leaf lily in winter

After sowing, move the plastic seedling trays into the winter greenhouse and maintain them in the greenhouse. The temperature in the greenhouse is controlled at 10-15°C at night and 20-25°C during the day; The seedlings emerge neatly, and the hymexazol 3000 times liquid is sprayed once 7-10 days after emergence to prevent damping-off. Due to the use of plastic seedling trays for sowing, there are fewer weeds, and a small amount of weeds can be manually removed.

After 20 days, the emergence rate was measured, and the test results are shown in Table 2. The results showed that the germination rate of Lilium pine leaves seedlings after variable temperature treatment reached 93.33%, which was significantly different compared with the untreated control group.

Table 2 Seed emergence rate of Lilium pine leaves

deal with Germination rate(%) treatment group 93.33±4.24A control group 68.67±4.62B

Capital letters in the table represent the results of significant difference analysis, p≤0.01.

6. Artificial low temperature dormancy for Lily pine seedlings

On April 15, 2018, the Lilium pine seedlings grown for 4 months in the winter greenhouse were dug out together with the lower bulbs, the upper cotyledons and lower roots were removed, and the treated bulbs were placed in a cool place to dry for 1 day. Put the dried bulbs of Lilium pine leaves into a ziplock bag, and put them in a refrigerator at 4°C to refrigerate to promote the dormancy of Lily pine leaves. The dormancy lasts for 60 days to mid-June. At the same time, 100 Lilium pine leaves were kept without low temperature dormancy treatment.

7. Release of dormancy and summer cultivation and management of Lilium pine leaves

On June 10, 2018, the bulbs of Lilium pine leaves after low-temperature dormancy were sown in seedling trays according to the above-mentioned step 5 (1) to remove dormancy, and the low-temperature dormancy treatment group and the untreated group were placed on the outdoor flat seedbed at the same time. On the seedbed, use glass fiber arches to set up a small shed. The arch height of the small shed is 0.8m and the span is 1.0m. The shed is covered with a shading net with a shading rate of 75%. Weed control is performed manually to maintain the cultivation in the plastic seedling tray. The substrate is moist but not waterlogged. Lilium pine leaves are cultivated and maintained in summer until October 20, 2018, after which lily pine leaves stop growing.

8. Lilium pine bulbs promote cultivation

In mid-October, dig out the lily bulbs of the low-temperature dormancy treatment group, and perform the second artificial low-temperature dormancy again according to the above step 6, dormant for 60 days until December 20, 2018, and then follow the above-mentioned step 5 (1) Place them in the greenhouse again for planting, and follow the above step 5 (2) winter greenhouse maintenance and management until April 15, 2019 to dig out the bulbs for processing. Finally, according to the above step 6, the third artificial low temperature dormancy was carried out on April 20, 2019 until June 15, 2019. The control group underwent wintering and maintenance management outdoors from June 15, 2018 to June 15, 2019. The growth status of the two groups was compared on August 1, 2019, and the results are shown in Table 3.

Table 3 Growth status of Lilium pine leaves

deal with Bulb weight (g) whether to bloom number of flowers Aboveground treatment group 5.07±0.54A yes 1-2 have erect stems control group 1.52±0.12B no 0 no erect stem

9. Open field cultivation and planting bed preparation of pine lily

Choose a higher place as a planting site to prevent water accumulation. The open-field cultivation seedbed of pine lily is 2.0m wide and 0.2m high. Before planting, the soil of the open-field cultivation seedbed of pine lily is removed from the soil of gravel, weeds and other garbage, and 3.0g of chlorfluoroclothianidin is evenly spread per square meter of seedbed to prevent grub damage.

Soak the lily bulbs in 0.5% carbendazim solution for 1 hour before planting, drain the water after soaking, and place them in a cool and ventilated place to dry in the shade for 1 hour to ensure that there is no moisture on the surface of the lily bulbs. The bulbs of Lilium pine leaves were planted according to the row spacing of 5.0cm×5.0cm, and the planting depth was 10.0cm.

10. Post-planting management of Matsuba lily

After the lily of the pine leaf is planted in the open field, it is not watered to prevent the bulbs from rotting. Natural rainfall can meet the water demand, as long as weeds are removed regularly. After the aboveground part grows, ensure sufficient light, spray 1000 times of potassium dihydrogen phosphate every 15 days, and stop spraying until it blooms in the same year.

The invention discloses a method for sowing and promoting cultivation of Lilium pine leaves. Those skilled in the art can learn from the content of this article and appropriately improve the process parameters to realize it. In particular, it should be pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The product of the present invention has been described through preferred embodiments, and relevant personnel can obviously make changes or appropriate changes and combinations to the product described herein without departing from the content, spirit and scope of the present invention to realize and apply the technology of the present invention .

Claims (3)

1. The method for sowing and forcing culture of the lilium pinifolium is characterized by comprising the following steps of:

step one, harvesting and pre-treating lilium pinifolium seeds;

harvesting in the next 9 th year when the capsules are withered and yellow and are not cracked, indoor ventilation drying for 15-20 days, taking out the seeds and removing immature seeds and impurities after the capsules are completely cracked, and storing the treated lilium pinnata seeds in a self-sealing bag;

step two, preparing the lily bulb at a low temperature before dry sand storage and storing the lily bulb at the low temperature;

screening the dried river sand with a soil sieve with a 1.0mm aperture, cleaning with running water, soaking for 30min with 0.5% potassium permanganate solution, washing with clear water for more than 3 times, airing the sterilized and cleaned fine sand in a metal disc, and controlling the water content to be below 1%;

uniformly mixing the lilium pinifolium seeds and the fine sand according to the volume ratio of 1:3, placing the mixture into a self-sealing bag or a sealed plastic box, and refrigerating at 4 ℃ for 60 days to 12 middle ten days;

step three, accelerating germination of the lilium pinosum;

taking out the seed sand mixture after 60 days of sand storage, soaking the seed sand mixture for 30min to 1h by using 1.0% carbendazim solution or 1.0% chlorothalonil solution, cleaning the seed sand mixture for 2 to 3 times by using clear water, soaking the seed sand mixture for 12 to 18h by using warm water at the temperature of 30 ℃ until white seed embryos in the middle of the lilium pinosum seeds can be clearly observed, pouring the soaked clear water, and then placing the seed sand mixture in a climatic chamber, and carrying out temperature change treatment for 5 days at the temperature of 25 ℃, 16h and 16 h;

step four, sowing and maintenance management of the lilium pinifolium in winter in a greenhouse;

(1) Winter greenhouse sowing of lilium pinifolium

Using a plastic seedling raising tray with a bottom drain hole to raise seedlings, and mixing the culture medium according to the volume ratio of turfy soil to perlite to decomposed deer manure of 3:1:0.5; placing a part of cultivation substrate in a plastic seedling tray, compacting, uniformly scattering and compacting the loose leaf lily seeds subjected to temperature change treatment and fine sand, so that the loose leaf lily seeds are fully contacted with the cultivation substrate; filling the plastic seedling raising tray with the residual culture medium and compacting; slowly irrigating and sterilizing with 3000 times of hymexazol;

(2) Winter greenhouse maintenance management for lilium pinifolium

After sowing, moving the plastic seedling raising tray into a winter greenhouse, wherein the temperature of the greenhouse at night is controlled to be 10-15 ℃ and the temperature of the greenhouse at daytime is controlled to be 20-25 ℃; keeping the culture medium moist but not accumulating water before emergence, and regularly emergence within 15-20 days, wherein the hymexazol 3000 times liquid is sprayed for 1 time 7-10 days after emergence;

step five, the lily seedlings are manually dormant at low temperature;

digging out the seedling of Song Yew lily together with the lower bulb in the middle 4 th year, removing the upper cotyledon and the lower root system, drying in the shade for 1 day, refrigerating at 4deg.C to promote dormancy of Song Yew lily, and dormancy for 60 days to middle 6 months;

step six, releasing dormancy of the lilium pinosum and cultivating, maintaining and managing in summer;

sowing the pine needle lily bulbs after dormancy at low temperature according to the fourth step to release dormancy in the middle ten days of 6 months; placing a plastic seedling raising tray outdoors in a flat Miao Chuangshang way, erecting an arch shed on a seedling bed, and covering the arch shed with a sunshade net with a sunshade rate of 75-95% to prevent and remove artificial weeds, so as to keep the culture medium in the plastic seedling raising tray moist but not accumulate water; culturing and maintaining the lilium pinifolium in summer until the period of 10 months is reached, and stopping growing the lilium pinifolium;

step seven, forcing culture of lilium pinifolium seed bulbs;

digging out the bulb of the lilium pinosum in the plastic seedling raising tray in the middle 10 months, carrying out the second artificial low-temperature dormancy according to the fifth step, dormancy for 60 days to the middle 12 months, planting and curing management according to the fourth step to the middle 4 months of the third year, and finally carrying out the third artificial low-temperature dormancy according to the fifth step;

step eight, open field cultivation of the lilium pinifolium and preparation of a planting bed;

and step nine, managing the planted lilium pinifolium.

2. The method for sowing and forcing culture of lilium pinifolium as claimed in claim 1, wherein the specific operation procedure of the step eight is as follows:

selecting a higher place as a planting field, removing broken stone and weeds in soil of a seedling bed for open field cultivation of the lilium Pini Maxim before planting, and uniformly spreading 3.0g of chlorflufenapyr on each square meter of seedling bed; soaking the lilium pinosum seed bulbs in 0.5% carbendazim solution for 30min-1h before planting, draining water after soaking, and drying in the shade for 1-2h at a cool and ventilated place to ensure that no water exists on the surfaces of the lilium pinosum seed bulbs; the lilium pinosum seed bulbs are planted according to the plant row spacing of 5.0cm multiplied by 5.0cm, and the planting depth is 8.0-10.0cm.

3. The method for sowing and forcing culture of lilium pinifolium as claimed in claim 2, wherein the specific operation procedure of step nine is as follows:

irrigation is not carried out after the open field planting of the lilium pinosum, and weeds are removed regularly; after the overground part grows, enough illumination is ensured, and 1000 times of potassium dihydrogen phosphate liquid is sprayed every 10-15 days until the flowers are opened in the current year to stop spraying.