CN103125238A - Multi-seedling autogenic relay growth method for seeds of amorphophallus muelleri - Google Patents
Multi-seedling autogenic relay growth method for seeds of amorphophallus muelleri Download PDFInfo
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- CN103125238A CN103125238A CN2013100615425A CN201310061542A CN103125238A CN 103125238 A CN103125238 A CN 103125238A CN 2013100615425 A CN2013100615425 A CN 2013100615425A CN 201310061542 A CN201310061542 A CN 201310061542A CN 103125238 A CN103125238 A CN 103125238A
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- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention relates to a multi-seedling autogenic relay growth method for seeds of amorphophallus muelleri, and belongs to the field of agricultural biotechnology. The multi-seedling autogenic relay growth method includes 1, processing the seeds; 2, soaking the seeds in solution containing 3% of potassium permanganate by 30 minutes to bactericide the seeds; 3, soaking the seeds in cold water for three days; 4, soaking the seeds in thiourea with the concentration of 0.1-3% or mixed liquid containing 400ppm GA3, 33ppm IBA and 33ppm 6-BA for 12 hours; and 5, breaking dormancy of the seeds and sowing the seeds. In the procedure for breaking the dormancy of the seeds and sowing the seeds for breeding seed bulbs, the moisture content of soil keeps 25-45% after the seeds are sown in the soil. As shown by test results, a germination rate of the seeds can reach 95% at least after seed dormancy breaking, an emergence rate of the seeds reaches 90% at least, the maximum weight of harvested seed bulbs of the amorphophallus muelleri is 710g, and the average weight of the harvested seed bulbs of the amorphophallus muelleri is 500g. The multi-seedling relay growth method has the advantages that a procedure is simple, and operation is convenient; by combining dormancy breaking with a germination accelerating and seedling raising mode, the germination speed and the germination rate of the seeds of the amorphophallus muelleri can be effectively increased, and the germination uniformity of the seeds of the amorphophallus muelleri can be effectively improved; and the quality of spouts is improved, the amorphophallus muelleri production period is shortened, and the method can be efficiently applied to large-scale production for commercial amorphophallus muelleri or seed bulbs in amorphophallus muelleri plantation.
Description
Technical field
The present invention relates to a kind of the grow directly from seeds method of many seedlings of seed kind consubstantiality relay growth of konjaku, belong to agricultural biological technical field.
Background technology
Konjaku be Araeceae (Araceae) Amorphophallus (
Amorphophallus) herbaceos perennial, be find so far unique can synthesize in a large number Glucomannan (
Konjac glucomannan, KGM) plant population, mainly be distributed in the torrid zone and the subtropical zone in Asia and Africa, only there is cultivation in minority Asian countries, China, Japan are more general.Araeceae Amorphophallus plant, after the process growth of 3-5, the phenomenon that the part konjak corm can occur blooming.The konjaku process of blossoming and having seeds is sexual propagation, its crossbreeding same plant male and female pistil self-pollination under natural environmental condition causes homophyletic sterile because of the gynoecium precocity, and the successful probability of different strain hybridization is extremely limited, cause konjaku fruit maturation rate extremely low, bloom the knot maturing rate less than 2%, obtain chance very low (Mekkerdchoo, the O. of seed kind
Et al.2012), therefore, konjaku is generally to adopt the way of the knot of blooming to obtain seed kind in batches, is to produce sowing by grow directly from seeds kind of ball and stripping and slicing in production, and this is also that traditional konjaku reproduction coefficient only has 3-6 main cause doubly, people are also carrying out tissue culture quick breeding research at present, but,, cost low due to its efficient high do not have to be applied (Wu Jin equality, 2007) in production reality still up to now.
The gold konjaku (
A. muelleri) be the domestication breeding kind of a kind of wild konjaku that remote border one band is found in country in Southeast Asia and China in recent years.Its stem tuber is rich in glucomannans, fresh tuber comprises the moisture of 75-80% and the total solids of 20-25%, carbohydrate (Glucomannan 78% left and right wherein, starch, colloid, oligosaccharides, disaccharide and monose etc. approximately 12%) 90.2%, ash content 4.0%, protein 3.3%, fiber 1.7% (Shimizu, N and Shimahara, H., 1984; Akesowan, A., 1991; Zhao. J.,
Et al., 2010; Zhao jianrong, 2011; 2012).And plant strain growth blade face gas green-ball stem (leaf-bulbil) aborning, have after many seedlings relay growth potential and growth potential seedling and floral leaf symbiosis before the strong mistake of seedling, the 39 pairs of triploids and be that the modes of reproduction (apomixes) of amixis produces a large amount of seed kinds by not pollinating after processing, the low biological property that waits uniqueness of stem tuber water content, make it possess high disease tolerance, high yield, the low height above sea level of growing area, and the characteristics of anti-storage and easy transportation.
At present, can adopt stable fast numerous producing method for seed for golden konjaku, by bulb size select, growth regulator sprays and naturally regulate, the seed kind is gathered etc., and technology is controlled, regulate the living golden konjaku of 1-4 (
A. muelleri) realize the rate of setting seeds more than 100% flowering rate and 300-2000 are doubly at the target bulb more than 200 grams, and seed kind maturing rate is up to 90%, realize golden konjaku (
A. muelleri) fast numerous production of hybrid seeds of stable scale.
Equally, in the propagating materials of konjaku seed kind, the growth potential of seed kind is exploited potentialities very large.But, under natural conditions, konjaku seed kind exists resting stage of 3-4 month and seedling not to tolerate weakness such as temperature lower than 10 ℃, yet, the inventive method is broken dormancy to realize that golden konjaku shifts to an earlier date 3-4 month and goes out the first seedling by the seed kind, adopt simultaneously corresponding temperature and humidity control measure to fall seedling stage to extend, for many seedlings relay growth provides the time.After golden konjaku seed kind has after the inventive method is processed, seedling growth potential is better than many seedlings relay growth characteristics of front seedling, can increase substantially leaf area index, thereby the growth cycle of ingenious prolongation plant, significantly increase the accumulation of biomass, then or next year become commodity taro (being not less than 500 grams).
The present invention to golden konjaku (
Amorphophallus muelleri) relay of many seedlings of the seed kind of growing directly from seeds consubstantiality grows into line correlation research, this method can effectively solve the dormancy of konjaku seed kind, the self-sow phase is short and do not tolerate the konjaku plant husbandry difficult problem such as low temperature freezing-disaster, for the development of konjaku industry provides good raw material supply.By literature search, have no the open report identical with the present invention.
List of references:
[1] Wu Jinping, Song Zhihong, Diao Ying, Xiang Fayun, Ceng Xiangguo, Gu Yucheng, Hu Zhongli. the tissue of bulbil class konjaku is cultivated and Fast-propagation, plant physiology journal 2007, (5) 2:887-888.
[2]?Shimizu，N.?and?Shimahara,?H.?1984.?Preparation?of?konjac?flour.?U.K.?Patent?Specification?no.1350497
[3]?Akesowan,?A..?Production?of?kabuki?flour?elephant?yam?(
Amorphophallus?sp.)?for?some?food
products.[M.Sc.thesis].?Department?of?food?Technology,?Chulalongkorm?University.?Bangkok,
Thailand.?1991.119p.
[4]?Zhao?Jianrong.?Integrated?production?of?purified?konjac?flour.?2011.?Bangkok.?ISBN
974-184-258-9.
[5]?Zhao?Jianrong.,?Zhang,?D,?Srzednicki,?G.,?Kanlayanarat,?S.?and?Borompichaichartkul,?C.?The
Morphological?and?Growth?Characteristics?of?Amorphophallus?Muelleri?Blume-?a?Commercially
Important?Konjac?Species.?Acta?Horticulturae.?2010.?875:501-508
[6]?Zhao?Jianrong.,?D.?Zhang,?Zhao?Jianping,?Srzednicki,?G.,?Kanlayanarat,?S.
Borompichaichartkul,?C..?The?Morphological?and?Growth?Characteristics?of?Amorphophallus
Muelleri?Blume-?a?Commercially?Important?Konjac?Species.?Acta?Horticulturae.
(ISTP)?.2010.875:501-508
[7]Mekkerdchoo,?O.*,?Holford,?P.,?Srzednicki,?G.,?Prakitchaiwattana,?C.,?Borompichaichartkul,?C.,
Wattananon,?S.?and?Zhao?J..?Determination?of?relationships?and?genetic?variation?among
Amorphophallus?sp.?from?northern?part?of?Thailand.?Journal?of?Food?Engineering.?2012.
23(6):247-252..
Summary of the invention:
The object of the invention is to overcome the deficiency of prior art, and a kind of method that provides konjaku to grow directly from seeds many seedlings of seed kind consubstantiality relay growth.
The present invention to golden konjaku (
Amorphophallus muelleri) by breaking seed kind natural dormancy period to realize that konjaku shifts to an earlier date 3-4 month and goes out the first seedling, adopt simultaneously corresponding temperature and humidity control measure to fall seedling stage to extend, for many seedlings relay growth provides the time.After this method is processed, golden konjaku seed kind has many seedlings relay growth characteristics that rear seedling growth potential is better than front seedling, can increase substantially leaf area index, thereby the growth cycle of ingenious prolongation plant significantly increases the accumulation of biomass, then or next year become commodity taro (being not less than 500 grams).
The concrete steps of the inventive method are as follows:
A. gather full seed kind after konjaku fruit full maturity, then carry out classification and air-dry according to seed kind weight, when seed kind moisture content is 20%-30%, ventilating at 10-15 ℃ of temperature, it is stand-by to preserve;
B. konjaku seed kind is processed: the liquor potassic permanganate with 3% soaks seed kind 30min sterilization; Used again soaked in cold water 3 days, and changed water every day once, after seed soaking, seed kind surface moisture is dried; Soaked the seed kind 12 hours with 500ppmGA3+50ppmIBA+50ppm6-BA or 0.1-3% thiocarbamide afterwards;
C. prepare in the seedbed: be the seedbed with the seedling medium build in green house, water content remains on 25-45%; Described seedling medium is fertilizer: turfy soil: the mixture of soil=0.5: 1: 2;
D. sowing and seedling management: leave the ditch of dark 4-5 centimetre on the seedbed, the konjaku seed kind that will process through conventional vernalization is according to 40 centimetres of line-spacings, and seed kind spacing is specification capable the broadcasting in ditch equably of 30 centimetres, and blastopore covers the water of drenching after topsoil up; Sunshade net is pulled on the booth top, and trickle is 3-4 time weekly, keeps soil matrix moistening; After the seed kind is sprouted and exposed Shamian Island, keep the interior humidity of cool canopy at 75-80%, between temperature of shed 25-32 ℃, every 1-2 month spraying pesticide once;
E. gather: excavate after 15 days until the lodging of konjaku seedling self-sow, classification is preserved;
F. the percentage in above-mentioned steps is percent by volume.
Described fertilizer is animal wastes or the plant residue that becomes thoroughly decomposed.
Described agricultural chemicals is Avermectin 1000-1500 times liquid and carbendazim 600-800 times liquid by the mixed liquor that mixes at 1: 1.
Beneficial effect of the present invention is:
1. result of the test shows: seed kind breaking dormancy germination rate reaches more than 95%, and the seed kind is germinateed neat, and emergence rate reaches more than 90%, and effectively solve the dormancy of konjaku seed kind, the self-sow phase is short and does not tolerate the konjaku plant husbandry difficult problem such as low temperature freezing-disaster.
2. the simple and clear easily row of method, be easy to promote the use of aborning.
Embodiment
Embodiment 1
The gold konjaku (
Amorphophallus muelleri) method of many seedlings of the seed kind of growing directly from seeds consubstantiality relay growth:
A. gather full seed kind after konjaku fruit full maturity, then carry out classification and air-dry according to seed kind weight, when seed kind moisture content is 20%-30%, ventilating at 10-15 ℃ of temperature, it is stand-by to preserve;
B. konjaku seed kind is processed: the liquor potassic permanganate with 3% soaks seed kind 30min sterilization; Used again soaked in cold water 3 days, and changed water every day once, after seed soaking, seed kind surface moisture is dried; Soaked the seed kind 12 hours with 500ppmGA3+50ppmIBA+50ppm6-BA or 0.1-3% thiocarbamide afterwards;
C. prepare in the seedbed: be the seedbed with the seedling medium build in green house, water content remains on 25-45%; Described seedling medium is fertilizer: turfy soil: the mixture of soil=0.5: 1: 2;
D. sowing and seedling management: leave the ditch of dark 4-5 centimetre on the seedbed, the konjaku seed kind that will process through conventional vernalization is according to 40 centimetres of line-spacings, and seed kind spacing is specification capable the broadcasting in ditch equably of 30 centimetres, and blastopore covers the water of drenching after topsoil up; Sunshade net is pulled on the booth top, and trickle is 3-4 time weekly, keeps soil matrix moistening; After the seed kind is sprouted and exposed Shamian Island, keep the interior humidity of cool canopy at 75-80%, between temperature of shed 25-32 ℃, every 1-2 month spraying pesticide once;
E. gather: excavate after 15 days until the lodging of konjaku seedling self-sow, classification is preserved;
F. the percentage in above-mentioned steps is percent by volume.
Described fertilizer is animal wastes or the plant residue that becomes thoroughly decomposed.
Described agricultural chemicals is Avermectin 1000-1500 times liquid and carbendazim 600-800 times liquid by the mixed liquor that mixes at 1: 1.
This method was implemented at the excellent new varieties Breeding base of Kunming Dongchuan District dry-hot valley konjaku in 2011, and result is: emergence rate reaches more than 95%, plants ball weight and is up to 630g, average weight 504g.
Embodiment 2
The gold konjaku (
Amorphophallus muelleri) method of many seedlings of the seed kind of growing directly from seeds consubstantiality relay growth:
A. gather full seed kind after konjaku fruit full maturity, then carry out classification and air-dry according to seed kind weight, when seed kind moisture content is 20%-30%, ventilating at 10-15 ℃ of temperature, it is stand-by to preserve;
B. konjaku seed kind is processed: the liquor potassic permanganate with 3% soaks seed kind 30min sterilization; Used again soaked in cold water 3 days, and changed water every day once, after seed soaking, seed kind surface moisture is dried; Soaked the seed kind 12 hours with 500ppmGA3+50ppmIBA+50ppm6-BA or 0.1-3% thiocarbamide afterwards;
C. prepare in the seedbed: be the seedbed with the seedling medium build in green house, water content remains on 25-45%; Described seedling medium is fertilizer: turfy soil: the mixture of soil=0.5: 1: 2;
D. sowing and seedling management: leave the ditch of dark 4-5 centimetre on the seedbed, the konjaku seed kind that will process through conventional vernalization is according to 40 centimetres of line-spacings, and seed kind spacing is specification capable the broadcasting in ditch equably of 30 centimetres, and blastopore covers the water of drenching after topsoil up; Sunshade net is pulled on the booth top, and trickle is 3-4 time weekly, keeps soil matrix moistening; After the seed kind is sprouted and exposed Shamian Island, keep the interior humidity of cool canopy at 75-80%, between temperature of shed 25-32 ℃, every 1-2 month spraying pesticide once;
E. gather: excavate after 15 days until the lodging of konjaku seedling self-sow, classification is preserved;
F. the percentage in above-mentioned steps is percent by volume.
Described fertilizer is animal wastes or the plant residue that becomes thoroughly decomposed.
Described agricultural chemicals is Avermectin 1000-1500 times liquid and carbendazim 600-800 times liquid by the mixed liquor that mixes at 1: 1.
This method was implemented in konjaku varieties breeding base, Dehong prefecture awns city, Yunnan Province in 2012, and result is: emergence rate reaches more than 95%, planted ball weight and was up to 710g, average weight 535g.
Claims (3)
1. the golden konjaku method of many seedlings of seed kind consubstantiality relay growth of growing directly from seeds is characterized in that the concrete steps of the method are as follows:
A. gather full seed kind after konjaku fruit full maturity, then carry out classification and air-dry according to seed kind weight, when seed kind moisture content is 20%-30%, ventilating at 10-15 ℃ of temperature, it is stand-by to preserve;
B. konjaku seed kind is processed: the liquor potassic permanganate with 3% soaks seed kind 30min sterilization; Used again soaked in cold water 3 days, and changed water every day once, after seed soaking, seed kind surface moisture is dried; Soaked the seed kind 12 hours with 500ppmGA3+50ppmIBA+50ppm6-BA or 0.1-3% thiocarbamide afterwards;
C. prepare in the seedbed: be the seedbed with the seedling medium build in green house, water content remains on 25-45%; Described seedling medium is fertilizer: turfy soil: the mixture of soil=0.5: 1: 2;
D. sowing and seedling management: leave the ditch of dark 4-5 centimetre on the seedbed, the konjaku seed kind that will process through vernalization is according to 40 centimetres of line-spacings, and seed kind spacing is specification capable the broadcasting in ditch equably of 30 centimetres, and blastopore covers the water of drenching after topsoil up; Sunshade net is pulled on the booth top, and trickle is 3-4 time weekly, keeps soil matrix moistening; After the seed kind is sprouted and exposed Shamian Island, keep the interior humidity of cool canopy at 75-80%, between temperature of shed 25-32 ℃, every 1-2 month spraying pesticide once;
E. gather: excavate after 15 days until the lodging of konjaku seedling self-sow, classification is preserved;
F. the percentage in above-mentioned steps is percent by volume.
2. the golden konjaku according to claim 1 method of many seedlings of seed kind consubstantiality relay growth of growing directly from seeds, it is characterized in that: described fertilizer is animal wastes or the plant residue that becomes thoroughly decomposed.
3. the golden konjaku according to claim 1 method of many seedlings of seed kind consubstantiality relay growth of growing directly from seeds is characterized in that: described agricultural chemicals is Avermectin 1000-1500 times liquid and carbendazim 600-800 times liquid by the mixed liquor that mixes at 1: 1.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340420A (en) * | 2015-11-25 | 2016-02-24 | 临沧滇晟农林发展有限公司 | Method for catalyzing germination of seedling seeds of amorphophallus bulbifer |
| CN106385860A (en) * | 2016-08-31 | 2017-02-15 | 怒江滇鑫科技有限责任公司 | Method for processing konjak seeds |
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| CN107318305A (en) * | 2017-08-18 | 2017-11-07 | 安康学院 | A kind of konjaku true seed fast breeding method |
| CN108849344A (en) * | 2017-10-23 | 2018-11-23 | 恩施土家族苗族自治州农业科学院 | A kind of konjaku grows directly from seeds the high yield cultivating method of seed |
| CN108848742A (en) * | 2018-06-25 | 2018-11-23 | 云南大学 | A kind of konjaku bulb low-temperature germination processing method |
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| CN105340420A (en) * | 2015-11-25 | 2016-02-24 | 临沧滇晟农林发展有限公司 | Method for catalyzing germination of seedling seeds of amorphophallus bulbifer |
| CN106385860A (en) * | 2016-08-31 | 2017-02-15 | 怒江滇鑫科技有限责任公司 | Method for processing konjak seeds |
| CN106561714A (en) * | 2016-11-04 | 2017-04-19 | 昆明学院 | Seed coating for konjac seedling seeds, and preparation method thereof |
| CN106561444A (en) * | 2016-11-04 | 2017-04-19 | 昆明学院 | Konjac cultivating method |
| CN106561714B (en) * | 2016-11-04 | 2019-05-24 | 昆明学院 | Konjaku grows directly from seeds seed kind seed coat agent and preparation method thereof |
| CN107318305A (en) * | 2017-08-18 | 2017-11-07 | 安康学院 | A kind of konjaku true seed fast breeding method |
| CN107318305B (en) * | 2017-08-18 | 2020-12-25 | 安康学院 | Rapid breeding method for konjac seedling seeds |
| CN108849344A (en) * | 2017-10-23 | 2018-11-23 | 恩施土家族苗族自治州农业科学院 | A kind of konjaku grows directly from seeds the high yield cultivating method of seed |
| CN108849344B (en) * | 2017-10-23 | 2020-07-14 | 恩施土家族苗族自治州农业科学院 | High-yield cultivation method for konjak seedling seeds |
| CN108848742A (en) * | 2018-06-25 | 2018-11-23 | 云南大学 | A kind of konjaku bulb low-temperature germination processing method |
| CN108848742B (en) * | 2018-06-25 | 2021-05-04 | 云南大学 | Low-temperature germination accelerating treatment method for konjak bulbs |
| CN109548607A (en) * | 2018-12-14 | 2019-04-02 | 湖北美荷农业科技有限公司 | A kind of nutritive cube cultural method of konjaku bulbil |
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