CN104094902A - Indoor subculture breeding method for phenacoccus solenopsis tinsley - Google Patents
Indoor subculture breeding method for phenacoccus solenopsis tinsley Download PDFInfo
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Abstract
The invention discloses an indoor subculture breeding method for phenacoccus solenopsis tinsley. The method includes the steps: S1, cultivating malvaviscus arboreus as a host plant; S2, transferring phenacoccus solenopsis tinsley onto the malvaviscus arboreus; S3, breeding the phenacoccus solenopsis tinsley in a breeding room; S4, repeating S1 to S3 for subculture breeding of the phenacoccus solenopsis tinsley. The malvaviscus arboreus which serves as the host plant is the favorite food for phenacoccus solenopsis tinsley and is evergreen, high in survival rate and available for cutting propagation, so that breeding cost is reduced; the malvaviscus arboreus is high in bearing capacity and beneficial to breeding of the phenacoccus solenopsis tinsley; the indoor subculture breeding method for the phenacoccus solenopsis tinsley is simple in process and easy to operate.
Description
Technical field
The present invention relates to a kind of coccid raising method, particularly the indoor subculture raising method of the continuous mealybug of a kind of Japan.
Background technology
The continuous mealybug (Phenacoccus solenopsis Tinsley) of Japan, belongs to Homoptera, Pseudococcidae, and continuous Eriococcinae, Phenacoccus, comes from North America, is the pernicious invasion insect extensively distributing in a kind of world wide.In the whole world, 38 countries and regions have been distributed to; In China, comprise Taiwan in interior 14Ge provinces and cities, all there is its trace.
The continuous mealybug host range of Japan is wide, and 57 sections 149 belong to 207 Plants and are its host, contain the plants such as crops, ornamental plants, vegetables, shrub, fruit tree and weeds.With sucking mouth parts, insert phloem or mesophyll absorption nutrition, and inject noxious material, cause that host is sallow, depauperation, torsional deformation, even dead, the honeydew of excretion attracts ant, and cause the generation of dark mildew, reduce photosynthesis of plant, when serious, occur burning symptom, reduce plant product as the salability of fruit etc.; Except direct and consequential damages crops, it has increased and has prevented or eliminate the cost that they produce as quarantine object, has caused huge economic loss.In India and Pakistan, endanger because of it in cotton field, and loss is up to more than 40%, and some cotton field even has no harvest, and Cotton Industry is caused to calamitous strike, to cotton grower, brings tremendous economic loss.In order to stop it to propagate, reduce its harm, the Ministry of Agriculture and State Administration for Quality Supervision and Inspection and Quarantine are classified as quarantine object.
Risk analysis result shows, China three Nei most areas, large cotton region are the height normal region of this insect, and the International Cotton Advisory Committee analyses and prediction Japan next harm of continuous mealybug country will be Chinese, show that Qi China spreads having a high potential of spreading.
For this insect Yi China, how to distribute, and some flowers and economic crops are produced to the reality of harm, and expand the expection of harm, biological property is studied, to predict its occurrence tendency; Its prophylactico-therapeutic measures is studied, to control its diffusion, spread, be necessary very much and very urgent.And indoors artificial subculture is raised, obtain a large amount of examination worms, be the prerequisite of carrying out every research.The raising method of the Chinese patent 201210233516.1 continuous mealybugs of Yi Zhong Japan, discloses and take potato and raise the method for the continuous mealybug of Japan as foodstuff.But potato is limited to the bearing capacity of the continuous mealybug of Japan, in feeding process, potato seedling is easily dead, be unfavorable for the continuous mealybug breeding of Japan, and potato cost is higher.
Summary of the invention
The indoor subculture raising method that the object of this invention is to provide the continuous mealybug of Japan that a kind of cost is low, step is simple, the continuous mealybug amount to obtain of Japan is large.
For achieving the above object, according to an aspect of the present invention, provide the indoor subculture raising method of the continuous mealybug of a kind of Japan, comprised the following steps:
S1, the outstanding bell flower of cultivation are as host plant;
S2, the continuous mealybug of Japan is transferred to outstanding bell takes;
S3, in insectary Nei Dui Japan, continuous mealybug is raised;
S4, repeating step S1～S3, carry out subculture raising to the continuous mealybug of Japan.
Thus, outstanding bell is spent evergreen all the year round, and all right cottage propagation, has reduced feeding cost.Outstanding bell flower bearing capacity is large, is beneficial to the continuous mealybug breeding of Japan.The indoor subculture raising method step of the continuous mealybug of above-mentioned Japan is simple, easy operating.
In some embodiments, in step S1, when outstanding bell is spent high 18～20cm, pinch, when outstanding bell is spent high 45～50cm as host plant for raising the continuous mealybug of Japan.Thus, pinch and can promote outstanding bell flower collateral generation, when outstanding bell flower reach a certain height, for raising the continuous mealybug of Japan, can guarantee survival and the best bearing capacity of outstanding bell flower.
In some embodiments, in step S2, the continuous mealybug of Japan is female adult.Thus, female adult lays eggs at outstanding bell flower, realizes the breeding of the continuous mealybug of Japan.
In some embodiments, in step S2, the outstanding bell of every strain is taken and is shifted the continuous mealybug of 10Tou Japan, thereby makes the appropriate bearing capacity of the outstanding bell flower tool of every strain.
In some embodiments, in step S3, the raising condition of the continuous mealybug adult of Japan is: 29～31 ℃ of temperature, photoperiod 14L:10D, intensity of illumination 10000LX, relative moisture 40～60%, the raising condition of the continuous mealybug nymph of Japan is: 25～27 ℃ of temperature, photoperiod 14L:10D, intensity of illumination 10000LX, relative moisture 60～80%.Thus, above-mentioned raising condition can make the continuous mealybug of Japan have shorter development duration, shorter population doubling time, more offspring.
In some embodiments, in step S3, the development duration of the continuous mealybug of Japan is 20～23 days, during spend to for every two days outstanding bell to supplement a moisture, once, fertilizing amount is every strain 1.6～2.4g in every two weeks fertilising.Thus, keep the skin wet and apply fertilizer between feeding period outstanding bell flower, can guarantee the growth of outstanding bell flower.
In some embodiments, in step S3, arrange and support worm frame in insectary, supporting worm frame is multilayer, and outstanding bell flower is placed on supports on worm frame.Thus, outstanding bell flower multilayer is placed, and has saved space, and then has reduced costs.
Many group experiments can verify that the suitable raising condition of the continuous mealybug of Japan and the suitableeest host plant are outstanding bell flower below.
Test the continuous mealybug of 1 Japan and raise the checking of condition
The continuous mealybug of Japan is raised and is carried out in climatic cabinate, 15,20,25,30 and 35 ℃ of 5 thermogrades and 45%, 60%, 75%3 relative moisture are set and carry out dual factors Orthogonal Composite, establish altogether 15 groups of processing, the photoperiod is 14L: 10D, intensity of illumination 10000LX.
In the continuous mealybug farrowing Sheng phase of Japan, with in the same size, in good health first of soft writing brush picking, incubate 40 of nymphs (time≤24h hatching), be inoculated on stalwartness, the Chinese Hibiscu seedling without pesticide pollution, putting into climatic cabinate raises, every strain Chinese Hibiscu seedling is to repeat for 1 time, raises condition setting 5 strain Chinese Hibiscu seedling for every group.Regularly observe, record each worm quantity in age every day, to Chinese Hibiscu, keep the skin wet in time, every circumferentially Chinese Hibiscu sealing fertilizer once, the about 1.0g of every strain.Extremely all examination worms grow to adult, add up female, male worm quantity.
The female worm of the continuous mealybug of Japan by vegetative stage be divided into one age nymph, two age nymph, tritonymph and adult.One age nymph, long 0.71～0.73mm, wide 0.36～0.38mm.Two age nymph, long 0.75～1.10mm, wide 0.36～0.65mm.Tritonymph, long 1.02～1.73mm, wide 0.82～1.00mm.Adult, long 3.00～4.20mm, wide 2.00～3.10mm.
With 10 of the soft female adults of the aforementioned stalwartness of writing brush picking (female adult worm age≤24h), be inoculated on stalwartness, the Chinese Hibiscu seedling without pesticide pollution, to put into above-mentioned corresponding climatic cabinate and raise, every strain is to repeat for 1 time, every processing repeats for 5 times.Regularly observe every day, record one age nymph quantity after removed, keep the skin wet in time, once, the about 1.0g of every strain, until all female adults are dead for sealing fertilizer weekly.Composite fertilizer, total nitrogen content >=15%, available phosphorus content >=15%, effective potassium content >=15%, granularity 90%.
Table 1 is the survival rate of the continuous mealybug of Japan under different temp. and humidity.Can draw, one age nymph the most responsive to variations in temperature, during raising, answer special concern.Excess Temperature or too lowly all cause age nymph mortality.20～30 ℃, whole nymph survival rate is mostly more than 50.00%, and in this temperature range, nymph can survive mostly, 25 ℃ and the combination of 75% relative moisture, and nymph survival rate is up to 84.00%.
Table 2 is the development duration of the continuous mealybug of Japan under different temp. and humidity.Can draw, if the phase of going through in one, two, three ages approaches, each worm age and whole nymph development duration raise and fall after rising with temperature, and 25～30 ℃ of development durations are shorter, 30 ℃ and the combination of 45% relative moisture, and nymph is the shortest, is only 12.30 days.
Table 3 is the female worm percentage of the continuous mealybug of Japan, life-span and fertility under different temp. and humidity.Can draw, too high or too low for temperature, female worm percentage is all lower, 25 ℃ and the combination of 75% relative moisture, and female worm percentage is the highest, is 90.00%; 20 ℃ of female Life of Adults are the longest; 15,35 ℃, female worm can not farrow, 25 ℃ and the combination of 60% relative moisture, and 30 ℃ and the combination of 45% relative moisture, and female worm farrowing amount is all more than 400.00.
Table 4 is the life table parameters of the continuous mealybug of Japan under different temp. and humidity.Can draw, 30 ℃ and the combination of 45% relative moisture, Japan's average generation time of continuous mealybug is only 21.81 days, the population doubling time is 3.47 days, is relatively beneficial to the continuous mealybug of Japan and grows.
In sum, the suitable temp. and humidity in different worm age is different, and 25 ℃ and the combination of 75% relative moisture, be relatively beneficial to nymph growth, and the female worm more than obtaining relatively; 30 ℃ and the combination of 45% relative moisture, Japan's female worm fertility of continuous mealybug is strong, can obtain more offspring.Therefore, in larval phase, select 25 ℃ and the continuous mealybug of 75% relative moisture combination raising Japan, the adult stage, while raising up seed, is selected 30 ℃ and the continuous mealybug of 45% relative moisture combination raising Japan.
The survival rate of the continuous mealybug of Japan under the different temp. and humidity of table 1
Note: in table, data are mean value ± standard error, with the difference letter after column data, represents that following table is same through the multiple comparing difference of Duncan formula remarkable (P=0.05).
The development duration of the continuous mealybug of Japan under the different temp. and humidity of table 2
The female worm percentage of the continuous mealybug of Japan, life-span and fertility under the different temp. and humidity of table 3
The life table parameters of the continuous mealybug of Japan under the different temp. and humidity of table 4
Test the checking of the continuous mealybug host plant for rearing of 2 Japan
Japan continuous mealybug happiness food host determines: get the high nutrition pot culture of 10～15cm tomato, Chinese Hibiscu, beggar-ticks, Ipomoea batatas, potato, outstanding bell flower, each strain of cotton seedling, on laboratory table top, form a circle, 200 of the continuous mealybugs of Japan are placed by circle central authorities, under normal temperature laboratory, let alone freely to select host, the isolation of surrounding water is in case the continuous mealybug escape of Japan, after 3 days, record respectively the continuous mealybug quantity of the upper Japan of various hosts, every worm age is a processing, and every processing repeats for 5 times.
Table 5 is the selectivity of the continuous mealybug of Japan to host.Can draw the continuous food that mealybug is liked of outstanding Ling Huaweige Japan in age.
The preference of the continuous mealybug of table 5 Japan to host
Note: in table, the continuous mealybug of insect population percentage (%) Wei Mouchong Japan in age selects certain host's insect population quantity to account for the percentage of upper this worm instar mouth total amount of all hosts.
The continuous mealybug of Japan the determining of optimum host that grow:
With the continuous mealybug of in the same size, the healthy and strong Japan of soft writing brush picking, just incubating a nymph in age (time≤24h hatching) is connected on tomato, Chinese Hibiscu, beggar-ticks, Ipomoea batatas, potato, outstanding bell flower, cotton seedling, be put in raising (30 ± 1 ℃ of temperature in climatic cabinate, relative moisture 70 ± 8%, photoperiod 14L:10D, intensity of illumination 10000LX).It is to repeat for 1 time that every strain connects 40 of worms, and every kind of host repeats for 5 times.Regularly observe, record each worm quantity in age every day, to host plant, keep the skin wet in time, give host plant sealing fertilizer once weekly, the about 1.0g of every strain.Extremely all examination worms grow to adult, add up female, male worm quantity.
With 10 of the soft female adults of the aforementioned stalwartness of writing brush picking (female adult worm age≤24h), be connected on corresponding host seedling, put into climatic cabinate (30 ± 1 ℃ of temperature, relative moisture 70 ± 8%, photoperiod 14L:10D, intensity of illumination 10000LX) in, raise, it is to repeat for 1 time that every strain connects 10 of worms, and every kind of host repeats for 5 times.Regularly observe every day, record one age nymph quantity after removed, keep the skin wet in time, once, the about 1.0g of every strain, until all female adults are dead for sealing fertilizer weekly.
Table 6 is the survival rate that different hosts raise the continuous mealybug of Japan.Can draw, with beggar-ticks, Ipomoea batatas and outstanding bell flower, raise after the continuous mealybug of Japan, each worm age and whole nymph survival rate are all higher.
Table 7 is the development duration that different hosts raise the continuous mealybug of Japan.Can draw, with different hosts, raise the continuous mealybug of Japan, the phase of going through in each worm age approaches, and with outstanding bell flower, raises the continuous mealybug of Japan, and nymphal development is very fast, whole nymph only 11.67 days.
Table 8 is that the continuous mealybug of Japan takes food female worm percentage, life-span and the farrowing amount after different hosts.Can draw, with outstanding bell flower, raise the continuous mealybug of Japan, although female worm percentage is lower, female Life of Adult is shorter, and female worm fertility is very strong, up to 647.92/female, be significantly higher than other host.
Table 9 is that the continuous mealybug of Japan takes food the life table parameters after different hosts.Can draw, with outstanding bell flower, raise the continuous mealybug of Japan, its average generation time is the shortest, is only 21.17 days, and clean growth rate is 238.14, and population increases by 1 times, only needs 2.68 days.
To sum up show, Xuan Linghuawei Japan continuous mealybug happiness food host, and this host is the continuous mealybug of Japan optimum host that grows.
The different hosts of table 6 raise the survival rate of the continuous mealybug of Japan
The different hosts of table 7 raise the development duration of the continuous mealybug of Japan
The continuous mealybug of table 8 Japan takes food female worm percentage, life-span and the farrowing amount after different hosts
| Host | Female worm percentage (%) | Female Life of Adult (my god) | Farrowing amount (head) |
| Tomato | 69.08±2.40a | 41.03±1.42c | 199.83±11.18d |
| Chinese Hibiscu | 59.31±1.01b | 57.15±1.77a | 339.41±11.33c |
| Beggar-ticks | 61.25±2.14b | 62.10±2.77a | 195.33±11.65d |
| Ipomoea batatas | 61.00±2.23b | 40.99±1.29c | 171.09±6.24d |
| Potato | 69.00±1.05a | 57.13±2.06a | 503.33±13.12b |
| Outstanding bell flower | 53.55±2.29c | 45.44±1.53bc | 647.92±9.51a |
| Cotton | 62.26±2.50b | 49.07±1.04b | 336.80±12.95c |
The continuous mealybug of table 9 Japan takes food the life table parameters after different hosts
In sum, beneficial effect of the present invention is: Xuan Linghuawei Japan continuous mealybug happiness food host, and this host is the continuous mealybug of Japan optimum host that grows.Outstanding bell flower, as host plant, easily obtains, and after initial purchase, gets final product cottage propagation, has reduced feeding cost.Outstanding bell flower is woody plant, evergreen all the year round, can the anniversary for the continuous mealybug of Japan provides food, realize the uninterrupted breeding of the continuous mealybug of Japan, at any time for test provides neat worm source.Support the layering of worm frame and raise, saved space, greatly reduced cost.The continuous mealybug rearing method of Japan, the simple easy operating of step, equipment needed thereby is simple, and cost is low.
Embodiment
The indoor subculture raising method of the continuous mealybug of Japan of present embodiment comprises the following steps:
The indoor subculture raising method of the continuous mealybug of Japan, comprises the following steps:
S1, the outstanding bell flower of cultivation are as host plant;
S2, the continuous mealybug of Japan is transferred to outstanding bell takes;
S3, in insectary Nei Dui Japan, continuous mealybug is raised;
S4, repeating step S1～S3, carry out subculture raising to the continuous mealybug of Japan.
The indoor subculture of the continuous mealybug of Japan is raised basic facilities and is comprised outdoor solarium, indoor insectary and foster worm frame.
The long 20m in outdoor solarium, wide 8m, high 3m.Ground in outdoor solarium is divided into two furrow, and the wide passageway of 1.5m is stayed in centre.The top of outdoor solarium is clear glass, and surrounding is shrouded with 80 order stainless steel mesh.In outdoor solarium, is furnished with tap.
The long 6m in indoor insectary, wide 4m, high 3m.The wall of insectary is provided with window, and window screening is 80 order stainless steel gauzes.It is ventilated that 1 exhaust fan is installed in insectary, and 1.2 air conditioner is controlled temperature, and the microcomputer time-controlled switch controlled light time, humidifier is controlled relative moisture.
Foster worm frame in insectary is 10.Support the long 150cm of worm frame, wide 50cm, high 200cm.Each foster worm frame is divided into two-layer up and down, is highly respectively 1m, and every layer of bottom is the clear glass that 0.5cm is thick, and the fluorescent lamp of 5 36W is installed at every layer of top.Fluorescent lamp is connected with microcomputer time-controlled switch, one of every layer of outfit fluorescent lamp hand switch.
Below each step is explained in detail.
S1, the outstanding bell flower of cultivation are as host plant.Operate as follows.
Outstanding bell flower is cultivated required fertilizer and plant modifying agent: composite fertilizer, total nitrogen content >=15%, available phosphorus content >=15%, effective potassium content >=15%, granularity 90%; Spend soil for the general soil of spending; Root-inducing powder; Above material is commercially available, and brand and producer are free.
Miao Yuan: buy the outstanding bell flower of the nutrition pot culture seedling that 15cm is high from market.
Spend little transplantation of seedlings to obtain transplanted seedling on the outstanding bell of buying gained: again to purchase nutritive cube (21cm * 17cm), at the thick soil of spending of nutritive cube bottom paving 5cm, with scissors, wipe out the nutritive cube of buying seedling, the whole strain of seedling band soil is transplanted to nutritive cube, replenish the thick soil of spending of 10cm, remaining 2cm space, puts to outdoor Yang Chong solarium.Fine day, waters once every day, and the cloudy day waters once for every three days.Once, the about 2.0g of every strain, grows to 18～20cm and pinches every two weeks sealing fertilizer, breaks apical dominance, promotes collateral generation.Grow to 45～50cm for the continuous mealybug of indoor feeding Japan.
After growing up to, the outstanding bell flower seedling of buying obtains cuttage seeding by cottage propagation: spring, the old branch 10～15cm of clip stalwartness, bring to indoorly, with 0.02～0.05% root-inducing powder, soak the about 30s in branch lower end, then branch lower end is inserted to the husky bed 5cm left and right degree of depth.Husky bed is soft thin river sand, is layered in the plastic basin of long 50cm, wide 30cm, high 15cm, and with the moistening husky bed of the hand-held watering can of LT908, water is too much unsuitable, in order to avoid ponding, branch is downright bad.Be placed in indoorly, note observe, replenishing water and preserving moisture, roots for approximately 20 days in time.Then in outdoor solarium, by the method for transplanted seedling, transplant processing, grow to 45～50cm for the continuous mealybug of indoor feeding Japan.
As from the foregoing, outstanding bell flower, as host plant, easily obtains, and after initial purchase, gets final product cottage propagation, has reduced feeding cost.
S2, the continuous mealybug of Japan is transferred to outstanding bell takes.Operate as follows.
The preparation in the continuous mealybug of Japan source: in the first tenday period of a month in May, Chinese Hibiscu blade or branch from field acquisition with the female adult of the continuous mealybug of Japan, bring to insectary.Said process is noted protection, in order to avoid anthropochory is escaped, caused to the continuous mealybug of Japan.
45～50cm transplanted seedling that step S1 is obtained or cuttage seeding are put to the foster worm frame in insectary, with plastics holder, hold.With fine, soft fur pen, touch the continuous mealybug of Japan side on Chinese Hibiscu, its lancet is extracted from Chinese Hibiscu blade or branch, then with writing brush, transfer them on outstanding bell floral leaf sheet.10 of the female adults of every strain reception farrowing.
S3, in insectary Nei Dui Japan, continuous mealybug is raised.Operate as follows.
When being transferred to 10 female adults that outstanding bell takes and not laying eggs, the condition in insectary is: 29～31 ℃ of temperature, photoperiod 14L:10D, intensity of illumination 10000LX, relative moisture 40～60%.
When one age nymph occur that in a large number, after (egg capsule occur after 4～6 days), the condition of adjusting insectary is: 25～27 ℃ of temperature, photoperiod 14L:10D, intensity of illumination 10000LX, relative moisture 60～80%.
The development duration of the continuous mealybug of Japan is 20～23 days, during spend to for every two days outstanding bell to supplement a moisture, once, fertilizing amount is every strain 1.6～2.4g in every two weeks fertilising.
Treat that nymph grows to adult, continue to shift 10 female insects to new outstanding bell and take subculture raising.20～23 days per generations.Approximately 5500 of the continuous mealybug nymphs in one age of Mei Zhuke output Japan, or two age 5000 of nymphs, or 4500 of tritonymphs, or 2000 of female adults.Can be indoor scientific research provides a large amount of uniform Japan continuous mealybug, guarantees carrying out smoothly of scientific research.
Above-described is only some embodiments of the present invention.For the person of ordinary skill of the art, without departing from the concept of the premise of the invention, can also make some distortion and improvement, these all belong to protection scope of the present invention.
Claims (7)
- The indoor subculture raising method of the continuous mealybug of 1.Yi Zhong Japan, is characterized in that, comprises the following steps:S1, the outstanding bell flower of cultivation are as host plant;S2, the continuous mealybug of Japan is transferred to outstanding bell takes;S3, in insectary Nei Dui Japan, continuous mealybug is raised;S4, repeating step S1～S3, carry out subculture raising to the continuous mealybug of Japan.
- 2. the indoor subculture raising method of the continuous mealybug of Japan according to claim 1, is characterized in that, in step S1, when outstanding bell is spent high 18～20cm, pinch, when outstanding bell is spent high 45～50cm as host plant for raising the continuous mealybug of Japan.
- 3. the indoor subculture raising method of the continuous mealybug of Japan according to claim 1, is characterized in that, in step S2, the continuous mealybug of Japan is female adult.
- 4. the indoor subculture raising method of the continuous mealybug of Japan according to claim 3, is characterized in that, in step S2, the outstanding bell of every strain is taken and shifted the continuous mealybug of 10Tou Japan.
- 5. the indoor subculture raising method of the continuous mealybug of Japan according to claim 1, it is characterized in that, in step S3, the raising condition of the continuous mealybug adult of Japan is: 29～31 ℃ of temperature, photoperiod 14L:10D, intensity of illumination 10000LX, relative moisture 40～60%, the raising condition of the continuous mealybug nymph of Japan is: 25～27 ℃ of temperature, photoperiod 14L:10D, intensity of illumination 10000LX, relative moisture 60～80%.
- 6. the indoor subculture raising method of the continuous mealybug of Japan according to claim 1, is characterized in that, in step S3, the development duration of the continuous mealybug of described Japan is 20～23 days, spend to for every two days during this time outstanding bell and supplement a moisture, once, fertilizing amount is every strain 1.6～2.4g in every two weeks fertilising.
- 7. the indoor subculture raising method of the continuous mealybug of Japan according to claim 1, is characterized in that, in step S3, arranges and support worm frame in described insectary, and described foster worm frame is multilayer, and described outstanding bell flower is positioned over supports on worm frame.
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| CN110521674A (en) * | 2019-08-26 | 2019-12-03 | 广西壮族自治区农业科学院植物保护研究所 | A kind of indoor feeding method of Ban Shi encyrtid |
| CN111903617A (en) * | 2020-07-29 | 2020-11-10 | 广东省生物资源应用研究所 | Indoor subculture method of sedge |
| CN113349162A (en) * | 2021-07-09 | 2021-09-07 | 福建省农业科学院植物保护研究所 | Single-head indoor subculture method for Carica papaya mealybug |
| CN113349162B (en) * | 2021-07-09 | 2023-09-26 | 福建省农业科学院植物保护研究所 | Single-head indoor subculture method for papaya gecko |
| CN113439715A (en) * | 2021-07-30 | 2021-09-28 | 浙江省农业科学院 | Indoor successive transfer breeding method of lycoris radiata mealybugs |
| CN113678790A (en) * | 2021-07-30 | 2021-11-23 | 浙江省农业科学院 | Method for optimizing Babylonia wasp population structure in host conversion mode |
| CN113678790B (en) * | 2021-07-30 | 2023-02-24 | 浙江省农业科学院 | Method for optimizing Babylonia wasp population structure in host conversion mode |
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