CN104012476A - Method for massively raising laodelphax striatellus indoors - Google Patents
Method for massively raising laodelphax striatellus indoors Download PDFInfo
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- CN104012476A CN104012476A CN201410298198.6A CN201410298198A CN104012476A CN 104012476 A CN104012476 A CN 104012476A CN 201410298198 A CN201410298198 A CN 201410298198A CN 104012476 A CN104012476 A CN 104012476A
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Abstract
The invention provides a method for massively raising laodelphax striatellus indoors, belonging to the technical field of raising of insects. The method comprises the following steps: cultivating rice seedlings, raising three batches of laodelphax striatellus at different ages, changing seedlings and the like. According to the method, the rice seedlings can be cultivated by adopting a glass beaker and fine soil, and cultivation is not limited by sites, time, environments and other natural conditions; the three batches of laodelphax striatellus at different ages are raised simultaneously, so that the standard test insects with experimental ages can be provided at any time, the raising density of the three batches of insects can be strictly controlled, the full utilization of the rice seedlings and the acquisition of nutrition needed by the laodelphax striatellus can be guaranteed, not only can the changing times of the seedlings be decreased, but also the fertility of the laodelphax striatellus can be greatly improved; seedling change is carried out in an independent seedling changing room, and a black cloth capable of enabling the laodelphax striatellus to be phototropic is used for shielding, so that the mixing of different populations of laodelphax striatellus can be effectively avoided, and the purity of laodelphax striatellus population can be guaranteed. The method has the advantages of being simple and convenient as well as flexible to operate, consistent in insect ages, abundant, large in breeding quantity, stable to raise and the like, and can provide sufficient standard test insects for experiments.
Description
Technical field
The present invention relates to a kind of method of indoor mass rearing small brown rice planthopper and the application in agricultural thereof, belong to technical field of insect feeding.
Background technology
Small brown rice planthopper (Laodelphax striatellus
) genus Homoptera (Homoptera) Delphacidae, be distributed widely in the ground such as East Asia, Southeast Asia, Europe and north African, domestic distribution spreads all over various places, can sting suction and take food the multiple gramineous crops such as paddy rice, corn, wheat, barley, can also propagate various plants virus, the loss that its biography poison harm causes is far longer than direct thorn and inhales harm, is one of insect of being widely known by the people in current agricultural production.In recent years, due to the impact of the factor such as abuse and Climate Anomalies of the variation of cropping system, control medicament, small brown rice planthopper is lasting ascendant trend in the population density of various places, so small brown rice planthopper has been developed into the primary pest of Asia paddy rice since the 1950's, the virus disease of its propagation also becomes disease important on paddy rice.Stripe disease that there are some researches show Jiangsu generation in 2004 reaches 2,357 ten thousand mu, accounts for 79% of the Jiangsu Rice Cropping gross area, exceedes 5,000 ten thousand mu at present at national generation area, and No kernels or seeds are gathered, as in a year of scarcity in grave illness field.And the black streaked dwarf virus of rice of its propagation at present in Anhui, also there is distribution in Jiangxi and the north, Fujian in the interior central and east, the Yangtze river basin, since 2007, be outbreak of epidemic situation in various places, Jiangsu, be a destructive disease to whole rice workspace, caused huge economic loss.
Small brown rice planthopper becomes the huge obstacle of capturing high yield and bumper harvest in agricultural production gradually, is the primary study object of the plant protection departments of science and technology.Will to the harm feature of small brown rice planthopper itself, pass malicious characteristic, with host and virus mutual work and pesticide resistance, the pathogenesis of virus disease and pathogenic characteristic, the work of the qualification of resistant material and screening etc. aspect is furtherd investigate, and indoor mass rearing small brown rice planthopper is the basis of research.Therefore, must provide a large amount of small brown rice planthopper standard examination worms the anniversary, required with guarantee test.
Document announcement has the method for following several frequently seen indoor feeding plant hopper: 1, the indoor rice bud of a kind of rice fulgorid method for breeding.Introduced a kind of method of applying paddy rice rice bud and special foster worm device raising rice fulgorid, the method is because raising a large amount of small brown rice planthoppers with rice bud soilless-cultivated seedling, and seedling raise period is short and insect density is large, changes the seedling cycle thereby greatly shortened, and worm is also more difficult consistent age in addition.2, the method for north of china in winter indoor feeding small brown rice planthopper.Introduced equally application rice seedling and tillering stage wheat as host plant for rearing, turn worm case and dependent insect cage is raised with special, change the seedling cycle although this method has shortened, the utensils such as alms bowl dish take up room too large, operate still not too easy.3, the indoor amount reproduction of small brown rice planthopper and winter feeding method.Spring, summer, autumn raise with rice seedlings and raising box, and winter is with wheat and be with native vinyl disc to raise, and method of operating is comparatively complicated.4, a kind of short-cut method of indoor feeding small brown rice planthopper.Cultivate rice seedlings taking sponge as medium with plastic casing and raise, single seedling single head method obtains small brown rice planthopper in same length of time, and the worm amount that can provide is limited.5, the raising of small brown-back rice plant-hopper and raise poison.Article 1-3 for the raising of small brown rice planthopper with raise malicious method, complex operation and can not carry out many generations and raise, breeding cycle is long and a large amount of standard worms source cannot be provided.6, Preliminary report of healthy rearing plants for Laodelphax striatellus Fallen in indoor artificial feeding.The wheat of apparatus cane and barnyard grass are raised adult, raise nymph with wheat and barnyard grass seedling, and breeding cycle is long and be subject to the restriction such as place and temperature.Can find out from the rearing method of above several small brown rice planthoppers, selecting suitable host plant for rearing, instrument and place is the key of carrying out small brown rice planthopper artificial feeding.This research department is to small brown rice planthopper and through the two-strain (rice stripe virus of its propagation, Rice stripe virus, RSV) and (rice black-streaked dwarf virus, rice black-streaked dwarfvirus, RBSDV) on the basis of studying for many years, grope a set of can be in the method for indoor mass rearing small brown rice planthopper, the method is easy and simple to handle, the whole year raise, worm is neat age, raise and stablize continuously, a large amount of standards examination worms can be provided, greatly meet experiment needs.
Summary of the invention
Raise the deficiency of small brown rice planthopper method based on indoors artificial in existing data, object of the present invention aims to provide and is not a kind ofly subject to such environmental effects, easy and simple to handle, breeding cycle is short, the length of time is neat, reproduction rate is high, the annual method of raising continuously, the indoor feeding small brown rice planthopper of required worm age and worm amount can be provided at any time.
The method of described indoor mass rearing small brown rice planthopper, its feature comprises following processing step:
1) rice seed soaking and vernalization: get the some grains of full rice paddy seed, with clear water rinsing and remove shrivelled seed, use again Seed soaking 48h, after 48h, repeatedly rinse with clear water, pour in ceramic whiteware dish, add a small amount of clear water and be laid in vernalization in 24-28 DEG C of insectary, for moisturizing can cover a wet towel, after 48-60h, can be used to sowing.
2) cultivation of rice seedlings: clean in the 1000mL glass beaker drying and add preprepared fine earth (thick about 2cm), add water along walls of beaker.The seed of urging bud is sprinkling upon to soil uniformly upper, approximately 100 every glass, the fine earth of then spilling thin layer covers seed.With gauze covering beaker mouth tight with rubber band bundle.Broadcast the beaker of planting and be placed on the foster worm frame of insectary and grow, treat that rice seedlings grows to 6cm left and right and can be used for raising small brown rice planthopper.
3) small brown rice planthopper is raised in batches: raise in three batches small brown rice planthopper normal supporting under worm condition.Get some small brown rice planthopper nymphs for first batch and be placed in step 2) newly educate on rice seedlings totally 10 glasss; Getting some 3-4 small brown rice planthoppers in age for second batch is placed in and newly educates on rice seedlings, totally 10 glasss; Get some striatellus imagos for the 3rd batch and raise in newly educating on rice seedlings, totally 10 glasss.Every glass all covers gauze and ties and obturage with rubber band, on beaker, puts the worm date with black marking pen mark, is then positioned over to support normal management on worm frame and raise.Changed one time seedling every 8-10 days, while changing seedling, the beaker mouth that small brown rice planthopper is housed is tipped upside down on the new talent beaker cultivating, muffle interface with the black cloth of width 20cm, a length 40cm, with writing brush put in worm beaker gently small brown rice planthopper is brushed to newly educating on rice seedling below, on beaker, mark and put the worm date with black marking pen, just completed the seedling process of changing with the sealing of gauze rubber band.If the worm below 4 ages of the small brown rice planthopper of changing, that old seedling of originally raising small brown rice planthopper just abandons, and the small brown rice planthopper on new talent continues normal management and raises; If worm more than 4 ages of changing, old seedling will stay the growth of normally watering and treat that on rice seedling, the ovum of small brown rice planthopper is hatched completely, generally on rice seedling, there is successively the little nymph of the white hatching from changing can see Miao Zhi is counted 7 days, within 10 days, teleonymph is hatched completely substantially, then on seedling, treats nymph to be changed to and newly educates normal management raising on rice seedling according to quantity distribution principle for 2-3 days.
Described indoor mass rearing small brown rice planthopper method, is characterized in that step 1) in visually observe rice seed after vernalization 48h and whether show money or valuables one carries unintentionally, can sow if major part has showed money or valuables one carries unintentionally; Show money or valuables one carries unintentionally if having no to place again after 12h observes and wait to sow.
Described indoor mass rearing small brown rice planthopper method, is characterized in that step 2) in add water in when sowing soil moisteningly, if it is excessive to add water, seed can rot mouldy after sowing, be difficult for germination, and with foul smell.
Described indoor mass rearing small brown rice planthopper method, is characterized in that step 2) in insectary select heat insulation effect better size to be about 10m
2idle room, require water power easy to use, an in-built changes in temperature convertible frequency air-conditioner; Supporting worm frame is made up of stainless steel stent, transparent toughened glass, plant growth lamp, timer etc.
Described indoor mass rearing small brown rice planthopper method, is characterized in that step 2) middle culture of pests condition: temperature 24-28 DEG C, relative moisture 70-90%, light application time 14h, intensity of illumination 5000-7000LUX.
Described indoor mass rearing small brown rice planthopper method, is characterized in that step 2) in the management of watering after planting: after 48h, treat the seed rice 1cm left and right of being unearthed of germinateing, water water for the first time, be as the criterion so that soil is moistening; After every 48h observe water, treat seedling grow to can be used for small brown rice planthopper raise till.
Described indoor mass rearing small brown rice planthopper method, is characterized in that step 3) in normal to support worm condition be temperature 24-28 DEG C, relative moisture 70-90%, light application time 14h, intensity of illumination 5000-7000LUX.
Described indoor mass rearing small brown rice planthopper method, is characterized in that step 3) in first batch to raise the density of nymph be 300-400 head/cup; Second batch of density of raising 3-4 instar is 200-300 head/cup; The 3rd batch of density of raising adult is 100-200 head/cup, controls stocking density and can effectively control the growing way of raising rice seedling, makes small brown rice planthopper fully absorb nutrition, grows up strong and sturdy.
Described indoor mass rearing small brown rice planthopper method, is characterized in that step 3) in change seedling be select one with place the independent room that separates completely of raising small brown rice planthopper, be called and change between seedling.Seedling is easy to operate is advisable to change to change between seedling size, and the small brown rice planthopper that can avoid like this escaping out while changing seedling is sneaked into the insectary in and raises in small brown rice planthopper, the effectively homogeneity of the guarantee small brown rice planthopper population of raising.
Described indoor mass rearing small brown rice planthopper method, is characterized in that step 3) in normal management the most important thing is to control the amount of watering in raising, the general 48h of rice seedlings that is raising small brown rice planthopper waters water one time, the amount of watering is as the criterion with native complete wetting in cup; Before need to changing the cup of seedling, 24h can not water, and after rice seedlings and soil are all dry, just can change seedling, if wet words of changing seedling both, small brown rice planthopper can be bonded on writing brush dead, the soil at the cup end also can fall down sometimes, not only makes troubles to operation, also can cause the loss of the small brown rice planthopper number of animals raised.
According to said method raise, application beaker is cultivated rice seedlings, and the glass wall light transmission of beaker is better, and rice seedlings can absorb sufficient light source, grows comparatively vigorous, can provide sufficient nutrition for small brown rice planthopper; Also availability comparatively easy, quick, rice seedling is also high in the time changing the operations such as seedling for beaker in addition.Raise in three batches small brown rice planthopper, when just in time having ensured three kinds of worm states, exist, the experiment that the various worm states of can satisfying the demand are at any time carried out, and can be according to adjust at any time the worm scale of supporting by worm amount, enough standard examination worms were both provided, had been unlikely to again to cause the excess waste of worm amount.The small brown rice planthopper that this method is raised can be applicable to insect biology habit, insect and host's mutual work, a series of research such as excavation qualification, disease-resistant variety seed selection of variety source.
Brief description of the drawings
Fig. 1 is the insectary indoor scene schematic diagram of indoor mass rearing small brown rice planthopper.
Specific implementation method
Further illustrate the present invention below by specific embodiment.
In following examples, foodstuff source and infrastructure are as follows:
Paddy rice, kind " Wu-Yu-Geng 3 ", Plant Protection Inst., Jiangsu Academy of Agriculture is from numerous rice seed; Insectary: be about 10m depending on better size of heat insulation effect of scale selection
2idle room, require water power easy to use, an in-built changes in temperature convertible frequency air-conditioner; Support worm frame: 125cm × 40cm × 200cm support worm frame several, each shelf divides 4 layers, a transparent toughened glass that quality is thicker of every layer of pad, and three plant growth lamps that specification is 40W are installed at every layer of top, light source is downward, and each shelf is installed microcomputer time-controlled switch; Change between seedling: raise with placement the independent room that small brown rice planthopper separates completely for one, to change, seedling is easy to operate is advisable size, the built-in tables and chairs that can conveniently change seedling.
Embodiment 1
The method of indoor mass rearing small brown rice planthopper, carry out in accordance with the following steps:
1. cultivate rice seedlings: get full Wu-Yu-Geng 3 rice paddy seed, with clear water rinsing and remove shrivelled seed, use again Seed soaking 48h, after 48h, repeatedly rinse with clear water, pour in ceramic whiteware dish, add a small amount of clear water and be laid in vernalization in 24 DEG C of insectaries, for moisturizing can cover a wet towel, water breakthrough seed rice has showed money or valuables one carries unintentionally and can be used to sowing; When sowing, first in the clean beaker drying, add preprepared fine earth (thick about 2cm), add water soil is infiltrated along walls of beaker.The seed rice showing money or valuables one carries unintentionally is sprinkling upon uniformly to soil upper, approximately 100 every glass, the fine earth of then spilling thin layer covers seed again.With gauze covering beaker mouth tight with rubber band bundle; Broadcast the beaker of planting and continue to be placed on 24 DEG C of temperature, relative moisture 70%, light application time 14h, grows on the foster worm frame of intensity of illumination 5000LUX, treats that rice seedlings grows to 6cm left and right and can be used for raising small brown rice planthopper;
2. raise small brown rice planthopper: 24 DEG C of temperature, relative moisture 70%, light application time 14h, raises different worm three batches of small brown rice planthoppers in age under the insectary condition of intensity of illumination 5000LUX, and each batch raised 10 glasss of left and right, and first batch of density of raising nymph is 300/glass; Second batch of density of raising 3-4 instar is 200/glass; The 3rd batch of density of raising adult is 100/glass.Every glass all covers gauze and ties and obturage with rubber band, on beaker, puts the worm date with black marking pen mark, is then positioned over to support normal management on worm frame and raise.
3. change seedling: changing every 8 days and between seedling, changing one time seedling, while changing seedling, the beaker mouth that small brown rice planthopper is housed is tipped upside down on the new talent beaker cultivating, muffle interface with the black cloth of width 20cm, a length 40cm, with writing brush put in worm beaker gently small brown rice planthopper is brushed to newly educating on rice seedling below, on beaker, mark and put the worm date with black marking pen, just completed the seedling process of changing with the sealing of gauze rubber band.If the worm below 4 ages of the small brown rice planthopper of changing, that old seedling of originally raising small brown rice planthopper just abandons, and the small brown rice planthopper on new talent continues normal management and raises; If worm more than 4 ages of changing, old seedling will stay the growth of normally watering and treat that on rice seedling, the ovum of small brown rice planthopper is hatched completely, generally on rice seedling, there is successively the little nymph of the white hatching from changing can see Miao Zhi is counted 7 days, within 10 days, teleonymph is hatched completely substantially, then on seedling, treats nymph to be changed to and newly educates normal management raising on rice seedling according to quantity distribution principle for 2-3 days.
Embodiment 2
The method of indoor mass rearing small brown rice planthopper, carry out in accordance with the following steps:
1. cultivate rice seedlings: get full Wu-Yu-Geng 3 rice paddy seed, with clear water rinsing and remove shrivelled seed, use again Seed soaking 48h, after 48h, repeatedly rinse with clear water, pour in ceramic whiteware dish, add a small amount of clear water and be laid in vernalization in 26 DEG C of insectaries, for moisturizing can cover a wet towel, water breakthrough seed rice has showed money or valuables one carries unintentionally and can be used to sowing; When sowing, first in the clean beaker drying, add preprepared fine earth (thick about 2cm), add water soil is infiltrated along walls of beaker.The seed rice showing money or valuables one carries unintentionally is sprinkling upon uniformly to soil upper, approximately 100 every glass, the fine earth of then spilling thin layer covers seed again.With gauze covering beaker mouth tight with rubber band bundle; Broadcast the beaker of planting and continue to be placed on 26 DEG C of temperature, relative moisture 80%, light application time 14h, grows on the foster worm frame of intensity of illumination 6000LUX, treats that rice seedlings grows to 6cm left and right and can be used for raising small brown rice planthopper;
2. raise small brown rice planthopper: 26 DEG C of temperature, relative moisture 80%, light application time 14h, raises different worm three batches of small brown rice planthoppers in age under the insectary condition of intensity of illumination 6000LUX, and each batch raised 10 glasss of left and right, and first batch of density of raising nymph is 350/glass; Second batch of density of raising 3-4 instar is 250/glass; The 3rd batch of density of raising adult is 150/glass.Every glass all covers gauze and ties and obturage with rubber band, on beaker, puts the worm date with black marking pen mark, is then positioned over to support normal management on worm frame and raise;
3. change seedling: changing every 8 days and between seedling, changing one time seedling, while changing seedling, the beaker mouth that small brown rice planthopper is housed is tipped upside down on the new talent beaker cultivating, muffle interface with the black cloth of width 20cm, a length 40cm, with writing brush put in worm beaker gently small brown rice planthopper is brushed to newly educating on rice seedling below, on beaker, mark and put the worm date with black marking pen, just completed the seedling process of changing with the sealing of gauze rubber band.If the worm below 4 ages of the small brown rice planthopper of changing, that old seedling of originally raising small brown rice planthopper just abandons, and the small brown rice planthopper on new talent continues normal management and raises; If worm more than 4 ages of changing, old seedling will stay the growth of normally watering and treat that on rice seedling, the ovum of small brown rice planthopper is hatched completely, generally on rice seedling, there is successively the little nymph of the white hatching from changing can see Miao Zhi is counted 7 days, within 10 days, teleonymph is hatched completely substantially, then on seedling, treats nymph to be changed to and newly educates normal management raising on rice seedling according to quantity distribution principle for 2-3 days.
Embodiment 3
The method of indoor mass rearing small brown rice planthopper, carry out in accordance with the following steps:
1. cultivate rice seedlings: get full Wu-Yu-Geng 3 rice paddy seed, with clear water rinsing and remove shrivelled seed, use again Seed soaking 48h, after 48h, repeatedly rinse with clear water, pour in ceramic whiteware dish, add a small amount of clear water and be laid in vernalization in 28 DEG C of insectaries, for moisturizing can cover a wet towel, water breakthrough seed rice has showed money or valuables one carries unintentionally and can be used to sowing; When sowing, first in the clean beaker drying, add preprepared fine earth (thick about 2cm), add water soil is infiltrated along walls of beaker.The seed rice showing money or valuables one carries unintentionally is sprinkling upon uniformly to soil upper, approximately 100 every glass, the fine earth of then spilling thin layer covers seed again.With gauze covering beaker mouth tight with rubber band bundle; Broadcast the beaker of planting and continue to be placed on 28 DEG C of temperature, relative moisture 90%, light application time 14h, grows on the foster worm frame of intensity of illumination 7000LUX, treats that rice seedlings grows to 6cm left and right and can be used for raising small brown rice planthopper;
2. raise small brown rice planthopper: 28 DEG C of temperature, relative moisture 90%, light application time 14h, raises different worm three batches of small brown rice planthoppers in age under the insectary condition of intensity of illumination 7000LUX, and each batch raised 10 glasss of left and right, and first batch of density of raising nymph is 400/glass; Second batch of density of raising 3-4 instar is 300/glass; The 3rd batch of density of raising adult is 200/glass.Every glass all covers gauze and ties and obturage with rubber band, on beaker, puts the worm date with black marking pen mark, is then positioned over to support normal management on worm frame and raise.
3. change seedling: changing every 8 days and between seedling, changing one time seedling, while changing seedling, the beaker mouth that small brown rice planthopper is housed is tipped upside down on the new talent beaker cultivating, muffle interface with the black cloth of width 20cm, a length 40cm, with writing brush put in worm beaker gently small brown rice planthopper is brushed to newly educating on rice seedling below, on beaker, mark and put the worm date with black marking pen, just completed the seedling process of changing with the sealing of gauze rubber band.If the worm below 4 ages of the small brown rice planthopper of changing, that old seedling of originally raising small brown rice planthopper just abandons, and the small brown rice planthopper on new talent continues normal management and raises; If worm more than 4 ages of changing, old seedling will stay the growth of normally watering and treat that on rice seedling, the ovum of small brown rice planthopper is hatched completely, generally on rice seedling, there is successively the little nymph of the white hatching from changing can see Miao Zhi is counted 7 days, within 10 days, teleonymph is hatched completely substantially, then on seedling, treats nymph to be changed to and newly educates normal management raising on rice seedling according to quantity distribution principle for 2-3 days.
The selection of above embodiment temperature (table 1), intensity of illumination (table 2) and stocking density (table 3) is to rice seedling growing way, survival period based on different condition; Small brown rice planthopper is grown, is gone through more preferably selecting that the research of the impacts such as phase, survival rate, Life of Adult and ratio, egg hatching rate makes.
The impact of table 1 different temperatures on rice seedling and small brown rice planthopper indices
* note: " rice seedling is just gone through the phase " refers to that rice growing is to the number of days that grows to 6cm and can be used for raising small brown rice planthopper; " rice seedling survival period " refers to from placing small brown rice planthopper to the number of days that changes seedling.
The impact of table 2 different illumination intensity on rice seedling and small brown rice planthopper indices
* annotate same table 1
The impact of the different stocking densities of table 3 on rice seedling and small brown rice planthopper indices
* annotate same table 1
The impact (table 1) that condition of different temperatures is certain on the indices tool of rice seedling and small brown rice planthopper, it is shorter and shorter that temperature is just gone through the phase from 22-30 DEG C of visible rice seedling, but the survival period of rice seedling in the time of 30 DEG C because excess Temperature has reduced, the indices of observing again small brown rice planthopper as seen in the time that temperature is 24,26 and 28 DEG C survival rate, Life of Adult, female worm ratio and the egg hatching rate in the each worm of small brown rice planthopper age all a little more than other temperature, so select the temperature condition of 24-28 DEG C, both growing fine of rice seedlings can be ensured, viability and the fertility of small brown rice planthopper can be ensured again.The difference of intensity of illumination is less on the impact of small brown rice planthopper indices, is mainly the impact (table 2) on rice growth, and intensity of illumination is higher, and rice seedling just goes through that the phase is shorter and survival period is longer, so selection intensity of illumination is 5000-7000LUX.Also large (table 3) of the impact of the survival period of different stocking densities on rice seedling and small brown rice planthopper, the stocking density of 1-2 instar is below 400/glass time, and its survival rate is more than 60%; The stocking density of 3-4 instar is below 300/glass time, and its survival rate is more than 73%; The stocking density of 5-6 instar is below 200/glass time, its survival rate is more than 81%, under several worm different stocking densities in age, its ovum biography rate is all very high, consider and determined that the stocking density in 3 batches of different worm ages is respectively: stocking density 300-400 head/cup of 1-2 instar, stocking density 200-300 head/cup of 3-4 instar, stocking density 100-200 head/cup of 5-6 instar.
Utilize the present invention to raise small brown rice planthopper, be not subject to the restriction of natural conditions, manual control is supported worm condition, adjusts and supports worm scale at any time, has both met the demand of experiment, can not cause again waste.The small brown rice planthopper of raising can be applicable to the various aspects of insect and the virus research that passes thereof.
Above-mentioned enforcement does not limit the present invention in any form.
Claims (1)
1. the method for an indoor mass rearing small brown rice planthopper, it is characterized in that: in the insectary of the certain condition of culture of tool, educate rice seedlings with 1000mL glass beaker refinement earth culture, treat that seedling grows to 6cm left and right for raising small brown rice planthopper, small brown rice planthopper is raised three batches by worm age simultaneously, raises 10 glasss of left and right small brown rice planthopper nymphs for first batch, raise 10 glasss of left and right 3-4 small brown rice planthoppers in age for second batch, raise 10 glasss of left and right striatellus imagos for the 3rd batch, changing every 8-10 days and between seedling, changing one time seedling, while changing seedling, the beaker mouth that small brown rice planthopper is housed is tipped upside down on and newly grown seedlings on beaker, with a width 20cm, the black cloth of length 40cm is muffled interface, with writing brush put in worm beaker gently small brown rice planthopper is brushed to newly educating on rice seedling below, on beaker, mark and put the worm date with black marking pen, again with the sealing of gauze rubber band, the rice seedlings management of normally watering during this time, can obtain at any time quantity abundance and worm abundant small brown rice planthopper in age, the key of the method is: 1) culture of pests condition: temperature 24-28 DEG C, relative moisture 70-90%, light application time 14h, intensity of illumination 5000-7000LUX, 2) first batch of density of raising nymph is 300-400 head/cup, and second batch of density of raising 3-4 instar is 200-300 head/cup, and the 3rd batch of density of raising adult is 100-200 head/cup, 3) change seedling and carry out in changing between seedling, changing between seedling is an independent room separating completely with placement raising small brown rice planthopper, 4) grow seedlings and raise the amount that in the process of small brown rice planthopper, strictly control is watered, when sowing, in soil, adding water moistening, after planting water water one time every 48h, be as the criterion so that soil is moistening, the rice seedlings of raising small brown rice planthopper generally waters water one time every 48h, and the amount of watering is as the criterion with native complete wetting in cup, but the cup that need to change seedling can not water before 24h.
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| CN104621051A (en) * | 2015-02-12 | 2015-05-20 | 贵州省植物保护研究所 | Method for rearing sogatella furcifera indoors |
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| CN105875518A (en) * | 2016-06-07 | 2016-08-24 | 广西南宁益土生物科技有限责任公司 | Drawer type rice planthopper feeding box |
| CN106305632A (en) * | 2016-08-29 | 2017-01-11 | 河北省农林科学院植物保护研究所 | Large-scale breeding method for laodelphax striatellus |
| CN108157299A (en) * | 2017-12-29 | 2018-06-15 | 四川省农业科学院植物保护研究所 | The planthopper scale anniversary method for breeding |
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