CN102578051A - Method for artificially feeding Chilo suppressalis in room - Google Patents
Method for artificially feeding Chilo suppressalis in room Download PDFInfo
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- CN102578051A CN102578051A CN2012100684666A CN201210068466A CN102578051A CN 102578051 A CN102578051 A CN 102578051A CN 2012100684666 A CN2012100684666 A CN 2012100684666A CN 201210068466 A CN201210068466 A CN 201210068466A CN 102578051 A CN102578051 A CN 102578051A
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Abstract
The invention belongs to the technical field of biological reproduction and cultivation, particularly to a method for artificially feeding Chilo suppressalis in a room. Chilo suppressalis is a polyphagous boring insect, and the indoor artificial feeding of the Chilo suppressalis plays an important role in multiple fields such as assessment of pesticide and the like. The method of the invention mainly uses a ziplock bag with small size, commonly used in a lab, as an egg bed of the Chilo suppressalis, and not only the materials is easily accessible, but also the specific operation of collecting egg masses is simplified further, at the same time, the method uses a plastic box with size of 20*15*15(cm) as a container, so the space occupied by the whole oviposition equipment in the method is smaller than that during using rice seedlings. In the invention, an improved method for artificially feeding Chilo suppressalis in the room is provided, and oviposition process of the Chilo suppressalis adult is simplified in the process of artificially feeding the Chilo suppressalis in the room, and enough egg masses can be produced for going down to the future generation.
Description
Technical field
The invention belongs to biological self reproducing culture technique field, be specifically related to the method that a kind of indoors artificial is raised striped rice borer.
Background technology
Striped rice borer
Chilo suppressalis(walker) belonging to the Crambiinae of Lepidoptera Pyralidae, is China's insect pest of rice.Striped rice borer distributes very wide in China, except that not finding to cause harm as yet in the minority area, all there is distribution in north and south rice district, and is annual because the injured area of striped rice borer harm reaches 1,000 ten thousand hm2.Striped rice borer is a polyphagous pest-insect, except that hazard rice, also endangers crops such as wild rice stem, wild wild rice stem, sugarcane, Chinese sorghum, corn, wheat, barnyard grass, broad bean, rape.At present the research of striped rice borer is worked except open-air field; In the process of multiple researchs such as pesticide manufacturing, need the consistent insect population of a large amount of development progresses to give birth to experiments such as survey, and the indoors artificial of striped rice borer is raised and can satisfactory experiment worm source be provided for these experiments.
The at present general striped rice borer rearing method that uses all is on the rice seedling method for breeding basis that Shang Zhizhen equals to propose in 1979, to improve; The natural host that these methods are used striped rice borer such as paddy rice rice seedling, wild rice stem, water chestnut in the Chilo spp larvae stage is as larvae feeds; The stage of laying eggs of adult then all uses the paddy rice rice seedling to supply adult to lay eggs, and produces the blade acquisition pieces of an egg that pieces of an egg are arranged through collecting.But in actual mechanical process the preparatory period of rice seedling longer, and do not having condition to be difficult under the indoor cultivation condition preferably obtaining winter.Use the rice seedling raising need to lay eggs in addition and keep the higher levels of humidity more than 90% in the case,, can cause adult to be difficult for laying eggs if do not use humidifier to reach.
Summary of the invention
The object of the invention is to propose a kind of process of laying eggs that can simplify the striped rice borer adult, and can produce enough pieces of an egg are raised striped rice borer in order to the indoors artificial that goes down to posterity method.
The indoors artificial that the present invention proposes is raised the method for striped rice borer, and concrete steps are following:
1, selects new seed rice, soak seed and vernalization;
2, will be in the seed rice that vernalization is sprouted to be packed wide-mouth bottle into, the seed rice amount to high 1/3 place of bottle, is treated rice growth to 2-3cm approximately, with its feed as the raising striped rice borer;
3, will grow to the striped rice borer pieces of an egg of blackhead phase and put into the above-mentioned wide-mouth bottle cultivating rice seedling that is equipped with; Seal bottleneck with black cloth, placing temperature is 27 ± 1 ℃, illumination: be to hatch in the culturing room of 16:8 interlunation; The larva that hatches remains in the bottle, under similarity condition, raises;
4, when rice seedling is eaten up by Chilo spp larvae in the wide-mouth bottle; Change the wide-mouth bottle that another is equipped with new rice seedling, with new and old wide-mouth bottle bottleneck butt joint, at the joint of two wide-mouth bottles; And former wide-mouth bottle wraps up with black cloth; At the bottom of the wide-mouth bottle that fills new rice seedling, carry out illumination, utilize the phototaxis of striped rice borer, make Chilo spp larvae shift entering naturally and fill in the wide-mouth bottle of new rice seedling;
5, repetitive operation step 4 to Chilo spp larvae is pupated; Collecting the striped rice borer pupa, and be placed in the culture dish that is lined with wet water absorbent paper, is 27 ± 1 ℃ in temperature equally, and illumination: be under the 16:8 interlunation, it is grown be the striped rice borer adult;
6, the blotting paper in the shop, bottom of the box of laying eggs, and splash into water droplet, to keep certain humidity in the whole box of laying eggs; Get a fritter cotton and soak 5% hydromel, place small container (like beverage bottle cap), put into the box of laying eggs and supply the striped rice borer adult edible;
7, valve bag is cut into a slice that is of moderate size, puts into the box of laying eggs, size is advisable erecting in box; Male and female striped rice borer adult is put into the box (see figure 1) of laying eggs in the ratio of 1:1;
8, cover lay eggs box box mouth and cover lid with pricking foraminate plastic paper;
9, will lay eggs that to place temperature be 27 ± 1 ℃ to box, illumination: be under the condition of 16:8 interlunation, changes valve bag every day, collects pieces of an egg, changes cotton and add water for blotting paper;
The collection pieces of an egg are very convenient after using valve bag; Be that pieces of an egg on the valve bag are through after alcohol disinfecting dries; The part that does not need to have pieces of an egg is cut; As long as crooked valve bag, can pieces of an egg easily be separated, but and the valve bag of removing pieces of an egg clean dry after repetitive cycling use (see figure 2);
10, to place temperature be 27 ± 1 ℃ to pieces of an egg, illumination: be to be cultured to the blackhead after date under the condition of 16:8 interlunation; Repeat the larva raising process.
Among the present invention, the box of laying eggs can adopt the plastic casing of 20 x, 15 x 15 (cm) size.
Among the present invention; Use laboratory small size valve bag commonly used as striped rice borer ovum bed, not only the material acquisition is quite easy, and in the concrete operations of collecting pieces of an egg, more simplifies; Use general plastic casing as laying eggs box simultaneously, taking up room also of the whole device of laying eggs is little when using rice seedling.
Description of drawings
Fig. 1 is laid eggs in the box of laying eggs for the striped rice borer adult.
Fig. 2 stays the pieces of an egg that can easily separate on the valve bag.
Embodiment
The improvement step of this method is that mainly adult lays eggs the stage, and larval stage still with reference to the rice seedling method for breeding of Shang Zhizhen, is therefore only done concrete the introduction with the rice seedling feed in following raising method.
1, selects new seed rice, soak seed and vernalization;
2, will be in the seed rice that vernalization is sprouted to be packed wide-mouth bottle into, the seed rice amount to high 1/3 place of bottle, is treated rice growth to 2-3cm approximately, with its feed as the raising striped rice borer;
3, will grow to the striped rice borer pieces of an egg of blackhead phase and put into the above-mentioned wide-mouth bottle of cultivating rice seedling that is equipped with; Seal bottleneck with black cloth, placing temperature is 27 ± 1 ℃, illumination: be to hatch in the culturing room of 16:8 interlunation; The larva that hatches remains in the bottle, under similarity condition, raises;
4, when rice seedling in the bottle is eaten up by Chilo spp larvae, change the wide-mouth bottle that another is equipped with new rice seedling.With the butt joint of new and old wide-mouth bottle bottleneck, with two bottles joint, and former wide-mouth bottle wraps up with black cloth, at the bottom of the wide-mouth bottle that fills new rice seedling, carries out illumination, utilizes the phototaxis of striped rice borer, makes Chilo spp larvae shift entering naturally and fills in the wide-mouth bottle of new rice seedling;
5, repetitive operation 4 to Chilo spp larvae is pupated; Collecting the striped rice borer pupa, and be placed in the culture dish that is lined with wet water absorbent paper, is 27 ± 1 ℃ in temperature equally, and illumination: be under the 16:8 interlunation, it is grown be the striped rice borer adult;
Adult lays eggs the stage:
7,, drip 5-10 and drip in order to keep the humidity in the entire container in plastic box bottom shop layers of absorbent paper as the box of laying eggs;
8, get a fritter cotton and soak 5% hydromel, place a beverage bottle cap (daily life collection), put into the box of laying eggs and supply the striped rice borer adult edible;
9, valve bag is cut into a slice that is of moderate size, puts into the box of laying eggs, size is advisable erecting in box;
10, male and female striped rice borer adult is put into the box of laying eggs in the ratio of 1:1;
11, cover box mouth and cover lid (lid that uses in my experiment has big opening, therefore adds a cover perforated plastic paper and preserves moisture and breathe freely, and adds lid layer cloth according to the also available perforated plastic paper of the box concrete condition of laying eggs) with the plastic paper of pricking the hole;
12, the box of will laying eggs places 27 ℃, illumination: be under the condition of 16:8 interlunation, changes valve bag every day, collects pieces of an egg, changes cotton and add water for blotting paper;
Pieces of an egg on the valve bag are cut in the part that does not need to have pieces of an egg after drying through alcohol disinfecting, as long as crooked valve bag can easily separate pieces of an egg, but and the valve bag of removing pieces of an egg clean dry after repetitive cycling use;
13, pieces of an egg place 27 ℃ equally, illumination: be to be cultured to the blackhead after date under the condition of 16:8 interlunation, repeats the larva raising process.
Claims (3)
1. an indoors artificial is raised the method for striped rice borer, it is characterized in that concrete steps are following:
(1) selects new seed rice, soak seed and vernalization;
(2) will be in the seed rice that vernalization is sprouted to be packed wide-mouth bottle into, the seed rice amount to high 1/3 place of bottle, is treated rice growth to 2-3cm approximately, with its feed as the raising striped rice borer;
(3) will grow to the striped rice borer pieces of an egg of blackhead phase and put into the above-mentioned wide-mouth bottle cultivating rice seedling that is equipped with; Seal bottleneck with black cloth, placing temperature is 27 ± 1 ℃, illumination: be to hatch in the culturing room of 16:8 interlunation; The larva that hatches remains in the bottle, under similarity condition, raises;
(4) when rice seedling is eaten up by Chilo spp larvae in the wide-mouth bottle; Change the wide-mouth bottle that another is equipped with new rice seedling, with new and old wide-mouth bottle bottleneck butt joint, with the joint of two wide-mouth bottles; And former wide-mouth bottle wraps up with black cloth; At the bottom of the wide-mouth bottle that fills new rice seedling, carry out illumination, utilize the phototaxis of striped rice borer, make Chilo spp larvae shift entering naturally and fill in the wide-mouth bottle of new rice seedling;
(5) repetitive operation step (4) to Chilo spp larvae is pupated; Collecting the striped rice borer pupa, and be placed in the culture dish that is lined with wet water absorbent paper, is 27 ± 1 ℃ in temperature equally, and illumination: be under the 16:8 interlunation, it is grown be the striped rice borer adult;
(6) blotting paper in the shop, bottom of the box of laying eggs, and splash into water droplet, to keep certain humidity in the whole box of laying eggs; Get a fritter cotton and soak 5% hydromel, place small container, and small container is put into the box of laying eggs supply the striped rice borer adult edible;
(7) valve bag is cut into a slice that is of moderate size, puts into the box of laying eggs, size is advisable erecting in box; Male and female striped rice borer adult is put into the box of laying eggs in the ratio of 1:1;
(8) cover lay eggs box box mouth and cover lid with pricking foraminate plastic paper;
(9) will lay eggs that to place temperature be 27 ± 1 ℃ to box, under the condition of illumination: interlunation=16:8, change valve bag every day, collect pieces of an egg, and change cotton and add water for blotting paper;
(10) pieces of an egg being placed temperature equally is 27 ± 1 ℃, under the condition of illumination: interlunation=16:8, is cultured to the blackhead after date; Repeat the larva raising process.
2. indoors artificial according to claim 1 is raised the method for striped rice borer, it is characterized in that the said box of laying eggs adopts the plastic casing of 20 x, 15 x, 15 cm.
3. indoors artificial according to claim 1 is raised the method for striped rice borer, and it is characterized in that the operating procedure of said collection pieces of an egg is: the pieces of an egg on the valve bag are disinfected in alcohol, dry, crooked then valve bag can easily separate pieces of an egg.
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Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103609531A (en) * | 2013-11-21 | 2014-03-05 | 北京林业大学 | Artificial feeding method of fragrant pear euzophera pyriella |
| CN103652441A (en) * | 2013-12-02 | 2014-03-26 | 中国计量学院 | Chilo suppressalis artificial feed and preparation method and application thereof |
| CN103766288A (en) * | 2014-01-20 | 2014-05-07 | 中国热带农业科学院环境与植物保护研究所 | Coconut black-headed caterpillar mass propagation method |
| CN104604814A (en) * | 2015-02-17 | 2015-05-13 | 浙江省农业科学院 | Method for efficient chilo suppressalis breeding |
| CN104823926A (en) * | 2015-05-27 | 2015-08-12 | 贵州省植物保护研究所 | Method for identifying mating state of male rice-stem borers |
| CN105379681A (en) * | 2015-10-18 | 2016-03-09 | 浙江省农业科学院 | Method for monitoring parasitic effect of parasitic wasp parasitizing chilo suppressalis larva |
| CN105494269A (en) * | 2015-12-29 | 2016-04-20 | 广西壮族自治区农业科学院甘蔗研究所 | Method for raising sugarcane chilo infuscatellus snellen |
| CN106106377A (en) * | 2016-07-18 | 2016-11-16 | 青海省农林科学院 | A kind of method of indoor feeding Fructus Foeniculi thin wing open country snout moth's larva larva |
| CN106212385A (en) * | 2016-07-13 | 2016-12-14 | 扬州大学 | A kind of apparatus and method laid eggs for rice stem borer |
| CN106212386A (en) * | 2016-07-18 | 2016-12-14 | 青海省农林科学院 | A kind of field sizeization raises the method for Fructus Foeniculi thin wing open country snout moth's larva |
| CN109548755A (en) * | 2018-12-10 | 2019-04-02 | 江西省农业科学院植物保护研究所 | A kind of method for breeding of striped rice borer |
| CN109601490A (en) * | 2019-01-17 | 2019-04-12 | 江西省农业科学院水稻研究所 | A kind of anniversary efficient propagation method of striped rice borer strain |
| CN110024751A (en) * | 2019-05-21 | 2019-07-19 | 吉林省农业科学院 | A kind of striped rice borer breeding apparatus and method for breeding |
| CN110604102A (en) * | 2019-11-05 | 2019-12-24 | 江苏省农业科学院 | Chilo suppressalis standardized feeding and management method |
| CN110892882A (en) * | 2019-11-08 | 2020-03-20 | 浙江省农业科学院 | Food replacing device and method for improving survival rate of chilo suppressalis larvae |
| CN114145269A (en) * | 2021-12-07 | 2022-03-08 | 江苏省农业科学院 | Accurate large-scale feeding method for indoor rice seedlings of chilo suppressalis |
| CN112868606B (en) * | 2021-02-26 | 2022-04-22 | 广西壮族自治区农业科学院 | Method for indoor raising sugarcane striped rice borers or dibbler |
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Cited By (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103609531B (en) * | 2013-11-21 | 2015-03-04 | 北京林业大学 | Artificial feeding method of fragrant pear euzophera pyriella |
| CN103609531A (en) * | 2013-11-21 | 2014-03-05 | 北京林业大学 | Artificial feeding method of fragrant pear euzophera pyriella |
| CN103652441A (en) * | 2013-12-02 | 2014-03-26 | 中国计量学院 | Chilo suppressalis artificial feed and preparation method and application thereof |
| CN103652441B (en) * | 2013-12-02 | 2015-06-03 | 中国计量学院 | Chilo suppressalis artificial feed and preparation method and application thereof |
| CN103766288A (en) * | 2014-01-20 | 2014-05-07 | 中国热带农业科学院环境与植物保护研究所 | Coconut black-headed caterpillar mass propagation method |
| CN103766288B (en) * | 2014-01-20 | 2016-06-01 | 中国热带农业科学院环境与植物保护研究所 | The a large amount of propagation method of moth knitted by coconut |
| CN104604814A (en) * | 2015-02-17 | 2015-05-13 | 浙江省农业科学院 | Method for efficient chilo suppressalis breeding |
| CN104823926A (en) * | 2015-05-27 | 2015-08-12 | 贵州省植物保护研究所 | Method for identifying mating state of male rice-stem borers |
| CN105379681A (en) * | 2015-10-18 | 2016-03-09 | 浙江省农业科学院 | Method for monitoring parasitic effect of parasitic wasp parasitizing chilo suppressalis larva |
| CN105494269A (en) * | 2015-12-29 | 2016-04-20 | 广西壮族自治区农业科学院甘蔗研究所 | Method for raising sugarcane chilo infuscatellus snellen |
| CN106212385A (en) * | 2016-07-13 | 2016-12-14 | 扬州大学 | A kind of apparatus and method laid eggs for rice stem borer |
| CN106212386A (en) * | 2016-07-18 | 2016-12-14 | 青海省农林科学院 | A kind of field sizeization raises the method for Fructus Foeniculi thin wing open country snout moth's larva |
| CN106106377B (en) * | 2016-07-18 | 2019-07-12 | 青海省农林科学院 | A kind of method of the thin wing open country snout moth's larva larva of interior raising fennel |
| CN106106377A (en) * | 2016-07-18 | 2016-11-16 | 青海省农林科学院 | A kind of method of indoor feeding Fructus Foeniculi thin wing open country snout moth's larva larva |
| CN109548755B (en) * | 2018-12-10 | 2021-06-08 | 江西省农业科学院植物保护研究所 | Chilo suppressalis breeding method |
| CN109548755A (en) * | 2018-12-10 | 2019-04-02 | 江西省农业科学院植物保护研究所 | A kind of method for breeding of striped rice borer |
| CN109601490A (en) * | 2019-01-17 | 2019-04-12 | 江西省农业科学院水稻研究所 | A kind of anniversary efficient propagation method of striped rice borer strain |
| CN110024751A (en) * | 2019-05-21 | 2019-07-19 | 吉林省农业科学院 | A kind of striped rice borer breeding apparatus and method for breeding |
| CN110604102A (en) * | 2019-11-05 | 2019-12-24 | 江苏省农业科学院 | Chilo suppressalis standardized feeding and management method |
| CN110892882A (en) * | 2019-11-08 | 2020-03-20 | 浙江省农业科学院 | Food replacing device and method for improving survival rate of chilo suppressalis larvae |
| CN110892882B (en) * | 2019-11-08 | 2024-02-13 | 浙江省农业科学院 | Foodstuff replacing device and method for improving survival rate of Chilo suppressalis larvae |
| CN112868606B (en) * | 2021-02-26 | 2022-04-22 | 广西壮族自治区农业科学院 | Method for indoor raising sugarcane striped rice borers or dibbler |
| CN114145269A (en) * | 2021-12-07 | 2022-03-08 | 江苏省农业科学院 | Accurate large-scale feeding method for indoor rice seedlings of chilo suppressalis |
| CN114145269B (en) * | 2021-12-07 | 2023-02-03 | 江苏省农业科学院 | Accurate large-scale feeding method for indoor rice seedlings of chilo suppressalis |
| WO2023103304A1 (en) * | 2021-12-07 | 2023-06-15 | 江苏省农业科学院 | Accurate large-scale indoor breeding method for chilo suppressalis using rice seedlings |
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Application publication date: 20120718 |