CN101331868B - Artificial propagation method of minute pirate bugs - Google Patents
Artificial propagation method of minute pirate bugs Download PDFInfo
- Publication number
- CN101331868B CN101331868B CN200810118204XA CN200810118204A CN101331868B CN 101331868 B CN101331868 B CN 101331868B CN 200810118204X A CN200810118204X A CN 200810118204XA CN 200810118204 A CN200810118204 A CN 200810118204A CN 101331868 B CN101331868 B CN 101331868B
- Authority
- CN
- China
- Prior art keywords
- minute pirate
- pirate bugs
- adult
- ovum
- kidney bean
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- Y02P60/216—
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention provides a method for propagating Oriusminutus artificially, which comprises the following steps: cultivation of spawning substrate, feeding of host; preparation of instruments of production, preparation of artificial feedstuff and preparation of seeds are carried out; under the conditions of 25 DEG C, 75% of PH and 16L:8D of illumination, kidney bean buds are taken as the spawning substrate, and Tetranychus cinnabarinus or the artificial feedstuff is used for feeding; firstly, the kidney bean buds on which the Oriusminutus spawn is produced are placed in a large tray, and kidney bean seedlings with proper Tetranychus cinnabarinus are placed in the large tray by interpenetration, according to the age period of Oriusminutus, the number of the proper Tetranychus cinnabarinus is confirmed; then the Oriusminutus with the tray are placed in an insect breeding cage for feeding the Oriusminutus. Spawn needs 12-16 days to grow to be imago, and the production period is about 30 days. The method is applicable to feeding with large amount and being promoted, thus laying the solid foundation for producing Oriusminutus with large amount and providing great convenience to biological control.
Description
Technical field
The present invention relates to the artificial fecundation method of a kind of Anthocoridae insect, particularly a kind of artificial fecundation method of minute pirate bugs belongs to damage by disease and insect biological control technical field.
Background technology
A series of problems such as the residue of pesticide that the chemical control of damage by disease and insect brings, environmental pollution and pest resistance to insecticide are subjected to social concerns day by day.U.S. government's promulgation in 1993 reduces the government decree that agricultural chemicals uses; Sweden is the plan of successful implementation minimizing agricultural chemicals usage amount 50%, and Denmark and Holland have limited " action plan " and " the long-term crop protection is planned " of 1997～2000 minimizing agricultural chemicals usage amounts 50% respectively.China's " pollution-free food and biopesticide " is classified as 21 century by government and first develops agenda, and has formulated the use of restricted part highly toxic pesticide and the regulation of agricultural chemicals high residue amount in food and feed.The biological control of diseases and pests of agronomic crop, the comprehensive regulation are to realize reducing significantly the main path that chemical pesticide uses.
People increase day by day to the demand of organic food and pollution-free food, and price height, thereby stimulated the fast development of green agriculture, pollution-free food production enterprise increases suddenly, its means of prevention generally is that main other method of preventing and treating is auxilliary with the biological control, certainly will strong requirement be arranged to natural enemy insect, therefore quicken the artificial scale raising of natural enemy insect and have important use and promotional value with commercialization production.
In recent years, owing to, cause the burst of some small-sized less important insects such as thrips, aphid, mite class and aleyrodid etc. to take place, and be difficult to control to the depending on unduly and unreasonable use of chemical pesticide.And minute pirate bugs has the better prevention effect to these small insects just.Simultaneously, minute pirate bugs belongs to for maximum in the Anthocoridae also is a genus that comprises most species, spreads all over all over the world, has characteristics such as have a very wide distribution, adaptability is good, quantity is many, predatism is strong, is considered to the natural enemy insect that a class has utilization value very much.
Based on above-mentioned, the biological property of minute pirate bugs has been done a large amount of research both at home and abroad in recent years.Minute pirate bugs is the incomplete metamorphosis insect, experiences ovum-nymph-adult three phases its history of life, minute pirate bugs nymph general 3～5 ages.By to a series of research of minute pirate bugs, people are just attempting raising on a large scale.
Though, abroad the research of minute pirate bugs to be used early, main contents concentrate on aspects such as biology, taxonomy and molecular biology, and the research of artificial feeding aspect is less relatively, does not possess the ability of mass rearing minute pirate bugs as yet.Simultaneously, because the cause of technical protection, we are difficult to grasp external breeding technology.Therefore, for the development that adapts to green agriculture demand, be badly in need of finding out the method for the feasible mass rearing minute pirate bugs of a cover to minute pirate bugs.
Summary of the invention
The present invention has launched minute pirate bugs is bred The Research of Relevant Technology in a large number by using for reference forefathers' experience, therefore, has found out the prolific production method of a kind of minute pirate bugs, comprises the steps:
1) cultivation of oviposition substrate
Join soil: black earth, vermiculite mix with 1: 1: 1 ratio with turfy soil;
Plantation with cultivate: the soil that will prepare is packed in the pot for growing seedlings 6, waters sufficient water, and the embryo of kidney bean seed is imbedded downwards in the soil, regularly waters, and does not put into the case 2 ' of laying eggs before the back true leaf of waiting to sprout launches;
2) host's raising: after kidney bean seedling true leaf launches, remove terminal bud, insert Tetranychus cinnabarinus, with under 25 ℃, RH 75%, 16L:8D illumination condition, raise in the dependent insect cage 1 ', about 5～7d can be for raising;
3) preparation of artificial feed: with pollen, water, tussah chrysalis inclusion ratio is to be made at 1: 4: 16, adds 0.04% potassium sorbate in total amount, feeds as carrier with absorbent cotton;
4) production of hybrid seeds: will gather the minute pirate bugs that comes, the indoor breeding with bean aphid continues generation, collects the minute pirate bugs adult after single head raised for 3 generations, and adult mating rejuvenation is collected the minute pirate bugs ovum as seeding;
5) raising of minute pirate bugs:
, raise as oviposition substrate with the kidney bean bud with Tetranychus cinnabarinus and artificial feed;
Earlier have the kidney bean bud of minute pirate bugs ovum to put into big pallet 7 together with pot for growing seedlings 6 product, intert and put into the new kidney bean seedling of inoculating Tetranychus cinnabarinus of band, it is 2: 1 that Tetranychus cinnabarinus becomes the mite and the quantity ratio of minute pirate bugs ovum; Be with pallet 6 to place dependent insect cage 1 ' together then; At 25～27 ℃, the raising indoor feeding of RH 75%, 16L:8D illumination, regularly water and supplement feed about 10～15d adult of can gathering; Then, adult being moved into the case of laying eggs lays eggs;
6) adult mating rejuvenation: draw female adult worm in the dependent insect cage 1 ' with pooter, put into little dependent insect cage, the male imago of drawing again in another dependent insect cage 1 ' is put into same little dependent insect cage, treat in two days all adults to be moved in case 2 ' the printing opacity chamber 5 of laying eggs after the mating, and put into oviposition substrate and an amount of feed, collect an oviposition substrate storage every day or continue to expand numerous;
7) storage of adult: the minute pirate bugs adult of gathering is 15 ℃ of temperature, preliminary treatment 7d under the condition of illumination 8L:16D, its female adult worm 46d of can surviving in 10 ℃ refrigerator is at 15 ℃, the preliminary treatment 4d of 12D:12L, its female adult worm 98d of can in 5 ℃ refrigerator, surviving;
8) collection storage of ovum: collect the minute pirate bugs ovum together with host plant, the minute pirate bugs ovum in the oviposition substrate is refrigerated under 5 ℃ of conditions;
9) use in the field: ovum with the pouch packing, is positioned on the plant, and the adult that maybe will have the kidney bean bud directly is positioned on the plant, is used for biological control.
The raising of the described minute pirate bugs of step 5) is meant directly puts into soilless culture dependent insect cage 1 with the minute pirate bugs ovum " the kidney bean seedling on, intert again and put into the kidney bean seedling that has an amount of Tetranychus cinnabarinus, the quantity of Tetranychus cinnabarinus is according to determining the length of time of minute pirate bugs; At 25～27 ℃, the raising indoor feeding of RH 75%, 16L:8D illumination, regularly water and supplement feed about 10～15d adult of can gathering; Then, adult being moved into the case of laying eggs lays eggs.
Step 2) described Tetranychus cinnabarinus is raised and will suitably be controlled its growth rate, and tetranychid ovum phase and minute pirate bugs nymph in 1 age are met.
Be minute pirate bugs nymph in 1 to 2 age the length of time of the described minute pirate bugs of step 5), and the ratio of described nymph and Tetranychus cinnabarinus is 1: 10.
The described minute pirate bugs of step 5) length of time is minute pirate bugs nymph in 1 age, and described Tetranychus cinnabarinus is an ovum phase tetranychid.
The described adult mating of step 6) rejuvenation is meant the minute pirate bugs adult of field acquisition, and indoor feeding raises up seed and breeds after identifying, hands over rejuvenation mutually with original strain.
The described oviposition substrate of step 8) is a live body, regularly water to guarantee its survival, to guarantee the normal hatching of minute pirate bugs ovum.
The described field of step 9) is used and will be selected that vegetation is good, the place of lucifuge, early, place evening.
Use in the described field of step 9) is to place with ovum, and guarantees that there is an amount of feed minute pirate bugs hatching back near its hatching point.
Use in the described field of step 9) is to place with adult, and puts into an amount of kidney bean bud, to increase the breeding quantity of adult in the field, preferably places at dusk.
Propagation method of the present invention has following advantage:
1, propagation method of the present invention has improved survival rate and the rate of development of minute pirate bugs nymph;
2, propagation method of the present invention has increased minute pirate bugs adult pick-up rate and egg laying amount;
3, it is more convenient that propagation method of the present invention makes the raising of minute pirate bugs, and the adult pick-up rate increases relatively;
4, propagation method of the present invention also improves egg laying amount relatively;
5, the ratio of each nutrient component is result by long-felt in the feed used herein, and the proportioning of feed and the allotment of each nutrient component thereof are more reasonable, growing and lay eggs more favourable minute pirate bugs;
6, the oviposition substrate of the present invention's employing is more conducive to the development growth of minute pirate bugs and lays eggs;
7, the stocking density of propagation method employing of the present invention is more reasonable comparatively speaking, is more suitable for the raising of minute pirate bugs.As: under 25 ℃, RH 75%, 16L:8D illumination condition, as oviposition substrate, raise, from the egg development to the adult, need 12～16d, an about 30d of production cycle with Tetranychus cinnabarinus or artificial feed with the kidney bean bud.Every receptacle (10m
2) in once can breed about 200,000 of minute pirate bugs.
8, propagation method of the present invention, the corresponding measure that the field of grasp is put the time in a suitable place to breed, taked has guaranteed availability and the survival rate of minute pirate bugs ovum and adult.
9, also taked corresponding rejuvenation measure in the propagation method of the present invention, thereby offspring's individuality of minute pirate bugs is better, is more conducive to a large amount of breedings of minute pirate bugs.
10, propagation method of the present invention is well suited for mass rearing and popularization, for a large amount of productions of minute pirate bugs lay a solid foundation, for biological control provides great convenience.
Description of drawings
Fig. 1 is that minute pirate bugs is bred flow chart in a large number;
Fig. 2 is the dependent insect cage schematic diagram that the present invention raises minute pirate bugs;
Fig. 3 is the case schematic diagram of laying eggs that the present invention raises minute pirate bugs;
Fig. 4 is the soilless culture dependent insect cage schematic diagram that the present invention raises minute pirate bugs;
Fig. 5 is the influences of 4 kinds of oviposition substrate to the female egg laying amount of minute pirate bugs list;
Fig. 6 is the influences of 4 kinds of oviposition substrate to the minute pirate bugs female adult worm life-span;
Fig. 7 is the influences of 4 kinds of oviposition substrate to the minute pirate bugs egg hatching rate;
Fig. 8 is the influences of 4 kinds of oviposition substrate to minute pirate bugs the preoviposition period;
Fig. 9 is the influences of 4 kinds of oviposition substrate to the minute pirate bugs egg-laying period;
Figure 10 be on 4 kinds of oviposition substrate per day egg laying amount curve ratio.
Embodiment
1, the tool of production
1) pooter:
Big pooter: once suck bull adult or nymph.Get interior diameter and be 2.5cm, the high rubber stopper that is complementary for the lucite tube of 10cm and two sizes and the mouth of pipe, make a call to the hole of a 8mm in the central authorities of each rubber stopper, pipette with 2ml inserts respectively in two rubber stoppers then, one of the method for inserting is that the tip of pipette is inserted from the little end of rubber stopper diameter, another is that the tip of pipette is inserted from the big end of rubber stopper diameter, is that the 4mm place cuts off (being beneficial to fluke) with the former pipette tip at diameter, inserts the end of lucite tube as fluke; The latter is pasting rubber stopper with cutter the tip of pipette is cut off, and encases the little end insertion lucite tube (preventing in the worm suction inlet) of rubber stopper diameter with 120 purpose gauzes, with the medical rubber pipe box on the pipette end, as an air-breathing end.
Purposes: be mainly used in and draw a fairly large number of minute pirate bugs nymph or adult individuality.
Little pooter: once suck an adult or nymph.Getting one in the pipette of 2ml, is that the 4mm place cuts off with its tip at diameter, terminally encases and will get final product on the medical rubber pipe box with 120 purpose gauzes.
Purposes: mainly be to use when drawing a minute pirate bugs nymph or adult individuality.
2) dependent insect cage:
Since minute pirate bugs grow and survival depends on live plant, and raising instrument in the past only can not satisfy the live plant needed space of growing for small-sized utensil, in order to address these problems, the author has designed in test a kind of dependent insect cage of being convenient to operate 1 ' shown in Fig. 2 (A), by aluminum alloy frame is support, top is beneficial to printing opacity with glass capsulation, adopt glass sliding door 1 for the ease of the operation front, in order to prevent that the minute pirate bugs nymph from climbing out of, in the groove of sliding door, put into foam-rubber cushion, in addition, the bottom is sealed with hard plastic board for convenience of moving, all the other each faces are with nylon gauze 2 sealings.Dependent insect cage 1 ' in the use is shown in Fig. 2 (B).Product there is the kidney bean bud of ovum put into big pallet 7, is with pallet to place dependent insect cage 1 ' together then, subsequently dependent insect cage 1 ' is placed on the shelf in the receptacle together with pot for growing seedlings 6.So placement mainly is for ease of unified management and pile line operation.
Little dependent insect cage: manufacture craft is identical with big dependent insect cage, only is that size goes up difference.
3) case of laying eggs:
The inventor has made a kind of case 2 ' of laying eggs of being more convenient for operating simultaneously according to the phototaxis of minute pirate bugs adult, lays eggs case 2 ' as shown in Figure 3 in the use.This case 2 ' of laying eggs is divided into darkroom 4 and printing opacity chamber 5, and for easy to operate, glass sliding door 1 is all adopted in two Room, and the door in darkroom is stained with black film.Printing opacity chamber 5 other each faces cover nylon gauze, and the darkroom light tight black cloth of 4 usefulness is obturaged.Between two Room Gua ?the door curtain made of cloth, the door curtain made of cloth has only top to be fixed on the support between two Room, the bottom can move freely.During use adult and oviposition substrate are put into printing opacity chamber 5, the oviposition substrate that will have ovum and a small amount of adult moves into darkroom 4, and oviposition substrate is directly moved into darkroom 4 by the middle door curtain made of cloth, can avoid the escape of adult like this.
4) raising condition
25～30 ℃ of temperature, relative moisture (RH) 50%～70%, illumination is approximately the indoor of 14L:10D.Temperature controlling instruments is a KFR-35GW/22 type Haier air-conditioning, and the wet equipment of control is the Defensor humidifier, and light irradiation apparatus is common fluorescent lamp.
2, production method
The flow process of a large amount of propagation production as shown in Figure 1.Raising to minute pirate bugs describes below:
The cultivation of oviposition substrate
Join soil: black earth, vermiculite mix with 1: 1: 1 ratio with turfy soil;
Plantation with cultivate: the soil that will prepare is packed in the pot for growing seedlings 6, waters sufficient water, and the embryo of kidney bean seed is imbedded downwards in the soil, regularly waters, and does not put into the case 2 ' of laying eggs before the back true leaf of waiting to sprout launches;
Host's raising: after kidney bean seedling true leaf launches, remove terminal bud, insert Tetranychus cinnabarinus, with under 25 ℃, RH 75%, 16L:8D illumination condition, raise in the dependent insect cage 1 ', about 5～7d can be for raising;
Will suitably control the length of time of Tetranychus cinnabarinus in the feeding process of Tetranychus cinnabarinus, its ovum phase and minute pirate bugs nymph in 1 age are met, concrete operation method is a lot, as: by regulating the hatching of temperature, humidity control tetranychid ovum; After the hatching, the feed that control is fed was controlled the temperature and humidity in the receptacle etc. in the past, taked suchlike measure all can get a desired effect.
The preparation of artificial feed: with pollen, water, tussah chrysalis inclusion ratio is to be made at 1: 4: 16, adds 0.04% potassium sorbate in total amount, feeds as carrier with absorbent cotton;
The production of hybrid seeds: will gather the minute pirate bugs that comes, the indoor breeding with bean aphid continues generation, collects the minute pirate bugs adult after single head raised for 3 generations, and adult mating rejuvenation is collected the minute pirate bugs ovum as seeding;
The concrete raising of minute pirate bugs
Under 25 ℃, RH 75%, 16L:8D illumination condition, as oviposition substrate, promptly adopting the kidney bean bud is the oviposition substrate of minute pirate bugs mass rearing with the kidney bean bud.Raise with Tetranychus cinnabarinus or artificial feed.
To note in the feeding process:, prevent that the silk that the too much tetranychid of its low age nymph quilt produces from clinging and death according to definite suitable tetranychid quantity in the length of time of minute pirate bugs.Suitably the length of time of control Tetranychus cinnabarinus, its ovum phase and minute pirate bugs nymph in 1 age are met, can improve the survival rate of low age minute pirate bugs, because consumed energy is few in the predation process, so can accelerate its rate of development.
There is the kidney bean bud 20 of ovum to involve in a criminal case product and puts into big pallet 7 with pot for growing seedlings 6, intert that to put into kidney bean seedling 10 strains that have Tetranychus cinnabarinus (best, put into several strains at every turn, put into) by stages, place above-mentioned dependent insect cage together with pallet then, under 25 ℃, RH 75%, 16L:8D illumination condition, raise, regularly water and supplement feed, about 10～15d adult of can gathering.
Then, the kidney bean seedling that will have the adult of can gathering moves in the darkroom 4 of laying coop 2 ', in darkroom 4, put into repellent or put into attractant in printing opacity chamber 5, to impel adult to enter in the printing opacity chamber 5, and in printing opacity chamber 5, put into oviposition substrate and an amount of feed, after treating that most of minute pirate bugs are got back to printing opacity chamber 5, collect oviposition substrate, remaining a small amount of minute pirate bugs is collected with pooter, at this moment, the adult of collection promptly can be applicable to the field and prevents insect, perhaps, the mating rejuvenation that divides adult to be used for adult with the pooter drawing section, thus be used for breeding once more.
So repeat, wait adult to lay eggs after, collect the minute pirate bugs ovum together with oviposition substrate, need so to repeat 10-13d, general about tens days of the egg-laying period.
From the egg development to the adult, need 12～16d, an about 30d of production cycle.Every receptacle (10m
2) in once can breed about 200,000 of minute pirate bugs.
3, adult mating rejuvenation
Draw female adult worm in the dependent insect cage with pooter, little dependent insect cage is put in taking-up, the male imago of drawing again in another dependent insect cage is put into same dependent insect cage, treat in two days all adults to be moved in the case printing opacity chamber of laying eggs after the mating, and put into oviposition substrate and an amount of feed, collect an oviposition substrate storage every day or continue to expand numerous.
Earlier oviposition substrate is moved in the darkroom before receiving oviposition substrate, in the printing opacity chamber, put attractant or in the darkroom, put into repellent, treat to collect oviposition substrate after most of minute pirate bugs are got back to the printing opacity chamber, put back in the printing opacity chamber after remaining a small amount of minute pirate bugs collected with pooter, put into oviposition substrate and feed again, so repeat 10-13d (about tens days of egg-laying period).
Specify: the individuality in the general same dependent insect cage is same strain, adult mating for another strain of avoiding degenerating, in addition, owing to easily causing the minute pirate bugs strain to degenerate, the indoor feeding overlong time reduces fertility, so the minute pirate bugs of field acquisition regularly adult, indoor feeding raises up seed and breeds after identifying, replenishes germ plasm resource (strain), hands over rejuvenation mutually with original strain.
4, the minute pirate bugs adult is deposited processing
The minute pirate bugs adult is 15 ℃ of temperature, preliminary treatment 7d under the condition of illumination 8L:16D, its female adult worm 46d of can surviving in 10 ℃ refrigerator.At 15 ℃, the preliminary treatment 4d of 12D:12L, its female adult worm 98d of can in 5 ℃ refrigerator, surviving.
5, the collection of minute pirate bugs ovum and storage: after adult gives birth to ovum, all ovum are collected together, refrigerate under 5 ℃ of conditions together with oviposition substrate and minute pirate bugs ovum, its incubation rate can remain on more than 75% in the 15d.
6, use in the field: ovum is installed on the plant with the pouch branch, and the adult that maybe will have the kidney bean bud directly places on the plant, is used for biological control.
1, the tool of production: soilless culture dependent insect cage
Use big dependent insect cage to raise and find in the process of minute pirate bugs that the amount of watering is difficult to control, and be awkward, for this reason, the inventor has designed a kind of novel soilless culture dependent insect cage 1 ", the nothing soil dependent insect cage 1 in the use " as shown in Figure 4.Soilless culture dependent insect cage 1 " main body be aluminum alloy frame; the design of whole dependent insect cage mainly is divided into two parts; top part is the insectary; it is positioned at the part of growing seedlings above the hole; bind (being beneficial to printing opacity) with glass in the top of insectary; wherein a side be provided with the sliding door 1, the material of described sliding door 1 is a lucite, and pull and 1 be provided with handle, respectively be provided with two magnetic clasps on the described handle and on the framework, the magnetic clasp on the sliding door 1 is corresponding with the magnetic clasp on the framework, in order to prevent climbing out of of minute pirate bugs, four limits at door all are stained with sponge strip, and each side of all the other of insectary seals with nylon gauze 2.
Lower part is the nutrition liquid chamber, the following part in hole of promptly growing seedlings, wherein most important part is exactly the tank 3 of placement nutrient solution made of plastic, handle is arranged in the front of tank so that operation, the slideway that has supply flume to slidably reciprocate on the top of the shelf, other parts except the nutrition liquid chamber tank all adopt glass capsulation, and purpose is to reduce water evaporates.
Should note in the process of using cystosepiment around and will the sealing with absorbent cotton on every side of seedling hole, avoid minute pirate bugs to climb into the nutrition liquid chamber.In addition, tank slowly to be pulled out a little when adding water, avoid hurting plant root.
2, production method: the method for propagation production mainly as shown in Figure 1.Method of operating is earlier the minute pirate bugs ovum to be placed on the kidney bean seedling, after the ovum hatching, interts in the insectary that to put into kidney bean seedling 10 strains that have Tetranychus cinnabarinus (best, put into several strains at every turn, put into by stages), answer the points for attention other operating procedure and embodiment 1 described basically identical except that above-mentioned.
Use the soilless culture dependent insect cage, more convenient unified the processing and operation, even production scale also can not influence the hatching of survival rate, spawning rate and the ovum thereof of minute pirate bugs more greatly, on the contrary, it is more time saving and energy saving that management is got up, be well suited for mass rearing and popularization, for a large amount of productions of minute pirate bugs lay a solid foundation, for biological control provides great convenience.
1, experiment material and method
Experiment material: choose four kinds of oviposition substrate kidney bean buds, kidney bean leaf, moyashi and broad bean sprouts.
Specify: experimental instrument is used stereomicroscope (Motic-SFC-11C-N2GG): Maike Aodi Industry Group Co Ltd produces, it is used for observing counting ovum amount, and the tool of production in addition, other feeding materials and experimental technique are consistent with a large amount of productions.
2, result and analysis
1) selectivity of 4 kinds of oviposition substrate
Minute pirate bugs does not reach the significance level between the per day egg laying amount on moyashi and broad bean sprout, and all the other have all reached significance level between handling.Wherein the per day egg laying amount on broad bean sprout is minimum is 1.4, and per day egg laying amount mostly is 12.7 most on the kidney bean bud, is 7.8 on the kidney bean leaf, is 1.7 on the broad bean sprout, and is specifically as shown in table 1.
Table 1 minute pirate bugs is to the selectivity of 4 kinds of oviposition substrate
| Oviposition substrate | Average daily egg laying amount (grain) | Average daily egg laying amount maximum (grain) | Average daily egg laying amount minimum of a value (grain) |
| The kidney bean bud | 12.7±1.5a | 17.6 | 9.5 |
| The kidney bean leaf | 7.8±0.8b | 9.6 | 5.9 |
| Moyashi | 1.7±0.3c | 1.0 | 2.7 |
| Broad bean sprout | 1.4±0.3c | 0.2 | 2.2 |
Data are mean ± standard error in the table, and the SPSS one-way analysis of variance is used in statistical analysis, the different letter representations of the significance of difference between different disposal (P=0.05) after the numeral.
2) 4 kinds of oviposition substrate are to the influence of the female egg laying amount of minute pirate bugs list
In 4 kinds of oviposition substrate, the female egg laying amount of the list of minute pirate bugs is maximum on the kidney bean bud, is 68.3, and minimum on the moyashi is 48.4, and difference has reached significance level between the two, and it is not remarkable that other handles differences.The female egg laying amount size of minute pirate bugs list is followed successively by: kidney bean bud＞kidney bean leaf＞broad bean sprout＞moyashi, illustrate may kidney bean some feature (as surface texture, smell etc.) of bud be more suitable for minute pirate bugs and lay eggs, some feature of moyashi then is unsuitable for laying eggs or suppressing laying eggs, be not suitable as the oviposition substrate of minute pirate bugs, specifically as shown in Figure 5.
3) 4 kinds of oviposition substrate are to the influence in minute pirate bugs female adult worm life-span
The life-span of minute pirate bugs female adult worm difference on 4 kinds of oviposition substrate is not remarkable, and wherein the longest showing is 15.9d on the broad bean sprout, and the shortest showing is 14.3d on kidney bean bud and the moyashi, specifically see Table 2, and Fig. 6 shows.
4 kinds of oviposition substrate of table 2 to egg laying amount, the preoviposition period, egg-laying period and adult life-span influence
Data are mean ± standard error in the table, and the SPSS one-way analysis of variance is used in statistical analysis, the different letter representations of the significance of difference between different disposal (P=0.05) after the numeral.
4) 4 kinds of oviposition substrate are to the influence of minute pirate bugs egg hatching rate
As seen from Figure 7, the incubation rate of the ovum of minute pirate bugs is kidney bean bud 95%, kidney bean leaf 93%, broad bean sprout 81% and moyashi 76% from high to low successively on 4 kinds of oviposition substrate.Back both incubation rates relatively low reason be 4d later on wherein some matrix just begin to occur rotting or wilting phenomenon (it is comparatively serious wherein to rot), so the ovum on it can not hatch, incubation rate reduces.This kind phenomenon does not appear in the kidney bean bud in the test, and its incubation rate is the highest in 4 kinds of oviposition substrate, though its hatching peak appears at 3d than the slow 1d of kidney bean leaf, but during 5d on 2 kinds of oviposition substrate the incubation rate of minute pirate bugs ovum much at one, therefore estimating the kidney bean bud from the brooding time of ovum and incubation rate also is the suitable oviposition substrate of minute pirate bugs mass rearing.The hatching peak of minute pirate bugs ovum occurs as far back as 2d on the kidney bean leaf, and this may be that so temperature may be high slightly, therefore hatching will be early than other 3 kinds of matrix because vein wants thin than stem and bud; In addition, though the kidney bean leaf wilting phenomenon occurred in the process of test, owing to ovum mainly produces on main lobe arteries and veins and petiole, to the almost not influence of hatching of minute pirate bugs ovum, specifically as shown in Figure 7.
5) influence the preoviposition period that 4 kinds of oviposition substrate being to minute pirate bugs
Minute pirate bugs the preoviposition period be followed successively by from long to short: broad bean sprout, moyashi, kidney bean leaf, kidney bean bud.The shortest on the kidney bean bud is 2.2d, and the longest on the broad bean sprout is 2.9d, significant difference between the two.In addition, be 2.4d on the kidney bean leaf, and difference has also reached significance level between the broad bean sprout.All the other are difference not significantly (table 2) each other.
Illustrate that may contain the reproductive development that certain material is unfavorable for the East Asia minute pirate bugs on the broad bean sprout lays eggs, thereby delay its egg-laying period that only be not forced to lay eggs, this point just matches with the result that selectivity is laid eggs when having other suitable oviposition substrate.Do not contain on the kidney bean bud by contrast and be unfavorable for the material of laying eggs, the preoviposition period shorter, see that specifically Fig. 8 shows.
6) 4 kinds of oviposition substrate are to the influence in the egg-laying period of East Asia minute pirate bugs
Minute pirate bugs egg-laying period on the kidney bean leaf is the longest to be 11.0d, and the shortest on moyashi is 10.4d, but 4 kinds of matrix treatments differences not significantly (table 2).
The minute pirate bugs egg-laying period is the shortest on the moyashi as seen from Figure 9, the hardness that epidermal tissue can be described has very big influence to laying eggs of minute pirate bugs, minute pirate bugs trends towards laying eggs in tender place, this point is observed with the position of laying eggs and has been drawn same conclusion, so will guarantee the characteristic that oviposition substrate is tender as much as possible in the process of mass rearing.
7) the per day egg laying amount curve ratio of minute pirate bugs on 4 kinds of oviposition substrate
As seen from Figure 10, tangible bimodal curve has appearred on moyashi and the broad bean sprout, and be unimodal curve basically on kidney bean bud and the kidney bean leaf, the egg production peak time of East Asia minute pirate bugs on the kidney bean bud appears at 4d the earliest, kidney bean leaf and broad bean sprout all appear at 5d, then descend, and the per day egg laying amount of the 4d on the moyashi～8d are except that 6d slightly descends, other each day is comparatively steady, and peak appears at 8d.The per day egg laying amount of East Asia minute pirate bugs is the 3d～12d greater than date of laying eggs of 2 on kidney bean bud and the moyashi, and broad bean sprout is the 3d～13d, and the kidney bean leaf is the 3d～14d.From lay eggs curve as can be seen the East Asia minute pirate bugs lay eggs at the kidney bean bud comparatively concentrated, but difference is not obvious between 4 kinds of oviposition substrate.
Claims (7)
1. the artificial fecundation method of a minute pirate bugs comprises the steps:
1) cultivation of oviposition substrate
Join soil: black earth, vermiculite mix with 1: 1: 1 ratio with turfy soil;
Plantation with cultivate: the soil that will prepare is packed in the pot for growing seedlings (6), waters sufficient water, and the embryo of kidney bean seed is imbedded downwards in the soil, regularly waters, and does not put into the case (2 ') of laying eggs before the back true leaf of waiting to sprout launches;
2) host's raising: after kidney bean seedling true leaf launches, remove terminal bud, insert Tetranychus cinnabarinus, with under 25 ℃, RH 75%, 16L:8D illumination condition, raise in the dependent insect cage (1 '), 5～7d can be for raising;
3) preparation of artificial feed: with pollen, water, tussah chrysalis inclusion ratio is to be made at 1: 4: 16, adds 0.04% potassium sorbate in total amount, feeds as carrier with absorbent cotton;
4) production of hybrid seeds: will gather the minute pirate bugs that comes, the indoor breeding with bean aphid continues generation, collects the minute pirate bugs adult after single head raised for 3 generations, and adult mating rejuvenation is collected the minute pirate bugs ovum as seeding;
5) raising of minute pirate bugs:
, raise as oviposition substrate with the kidney bean bud with Tetranychus cinnabarinus and artificial feed;
Earlier have the kidney bean bud of minute pirate bugs ovum to put into big pallet (7) together with pot for growing seedlings (6) product, intert and put into the new kidney bean seedling of inoculating Tetranychus cinnabarinus of band, it is 2: 1 that Tetranychus cinnabarinus becomes the mite and the quantity ratio of minute pirate bugs ovum; Be with pallet (6) to place dependent insect cage (1 ') together then; At 25-27 ℃, the raising indoor feeding of RH 75%, 16L:8D illumination, regularly water and supplement feed 10～15d adult of can gathering; Then, adult being moved into the case of laying eggs lays eggs;
6) adult mating rejuvenation: draw female adult worm in the dependent insect cage (1 ') with pooter, put into little dependent insect cage, the male imago of drawing again in another dependent insect cage (1 ') is put into same little dependent insect cage, treat in two days all adults to be moved in case (2 ') the printing opacity chamber (5) of laying eggs after the mating, and put into oviposition substrate and an amount of feed, collect an oviposition substrate storage every day or continue to expand numerous;
7) storage of adult: the minute pirate bugs adult of gathering is 15 ℃ of temperature, preliminary treatment 7d under the condition of illumination 8L:16D, its female adult worm 46d of can surviving in 10 ℃ refrigerator is at 15 ℃, the preliminary treatment 4d of 12D:12L, its female adult worm 98d of can in 5 ℃ refrigerator, surviving;
8) collection storage of ovum: collect the minute pirate bugs ovum together with host plant, the minute pirate bugs ovum in the oviposition substrate is refrigerated under 5 ℃ of conditions;
9) use in the field: ovum with the pouch packing, is positioned on the plant, and the adult that maybe will have the kidney bean bud directly is positioned on the plant, is used for biological control.
2. the propagation method of claim 1, wherein step 2) described Tetranychus cinnabarinus raises and will control its growth rate, and tetranychid ovum phase and minute pirate bugs nymph in 1 age are met.
3. the described propagation method of claim 1, wherein the described adult mating of step 6) rejuvenation is meant the minute pirate bugs adult of field acquisition, the indoor feeding breeding after identifying that raises up seed is handed over rejuvenation mutually with original strain.
4. the described propagation method of claim 1, wherein the described oviposition substrate of step 8) is a live body, regularly water to guarantee its survival, to guarantee the normal hatching of minute pirate bugs ovum.
5. the described propagation method of claim 1, wherein the described field of step 9) is used and will be selected that vegetation is good, the place of lucifuge, early, place evening.
6. claim 1 or 5 described propagation methods, wherein to use be to place with ovum in step 9) described field, and guarantee that there is an amount of feed minute pirate bugs hatching back near its hatching point.
7. claim 1 or 5 described propagation methods, wherein the described field of step 9) use be with adult in placing at dusk, and put into an amount of kidney bean bud, to increase the breeding quantity of adult in the field.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810118204XA CN101331868B (en) | 2008-08-07 | 2008-08-07 | Artificial propagation method of minute pirate bugs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810118204XA CN101331868B (en) | 2008-08-07 | 2008-08-07 | Artificial propagation method of minute pirate bugs |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101331868A CN101331868A (en) | 2008-12-31 |
| CN101331868B true CN101331868B (en) | 2011-05-25 |
Family
ID=40194972
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200810118204XA Expired - Fee Related CN101331868B (en) | 2008-08-07 | 2008-08-07 | Artificial propagation method of minute pirate bugs |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101331868B (en) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011010308A2 (en) | 2009-07-23 | 2011-01-27 | Bio-Fly Ltd | System and method of feeding beneficial insects |
| CN101810155A (en) * | 2010-04-27 | 2010-08-25 | 南京农业大学 | Tetranychina harti feeder |
| CN101940268B (en) * | 2010-09-16 | 2013-05-08 | 北京市农林科学院 | Micro capsule type artificial feed suitable for orius sauteri |
| CN102293182B (en) * | 2011-07-18 | 2013-04-03 | 山东省农业科学院植物保护研究所 | Orius sauteri raising method |
| CN102499180A (en) * | 2011-10-19 | 2012-06-20 | 淮海工学院 | New method of stocking Clanis bilineata |
| CN102440227A (en) * | 2011-11-16 | 2012-05-09 | 广东省烟草南雄科学研究所 | Method of breeding harpactor fuscipes |
| CN102599369B (en) * | 2012-03-13 | 2013-03-20 | 中国农业科学院烟草研究所 | Artificial feed for myzus persicae |
| CN104222010B8 (en) * | 2013-06-21 | 2016-12-07 | 中国农业科学院植物保护研究所 | A kind of artificial a large amount of methods producing predatism fleahopper |
| CN104222006B8 (en) * | 2013-06-21 | 2016-12-21 | 中国农业科学院植物保护研究所 | A kind of artificial a large amount of methods producing minute pirate bugs |
| CN103704176B (en) * | 2013-11-29 | 2015-05-06 | 贵州大学 | Orius strigicollis raising method |
| CN104365563B (en) * | 2014-12-04 | 2016-06-15 | 广西大学 | Multi-functional retractable dependent insect cage and for raising the method for breeding of bright red hair speckle moth |
| CN104938429B (en) * | 2015-06-30 | 2017-04-05 | 山东省农业科学院植物保护研究所 | A kind of method for raising Orius |
| CN105248382A (en) * | 2015-11-01 | 2016-01-20 | 贵州大学 | Breeding method and device of orius similis |
| CN105309393A (en) * | 2015-12-01 | 2016-02-10 | 贵州省植物保护研究所 | Novel ecological system breeding method for orius minutus |
| CN106106379A (en) * | 2016-08-09 | 2016-11-16 | 华中农业大学 | Apparatus and method for artificial breeding's Similis |
| CN106719475B (en) * | 2016-12-13 | 2019-11-15 | 浙江省农业科学院 | The producing method for seed of Female silkworm parthenogenetic clones |
| CN107410217B (en) * | 2017-05-16 | 2023-06-06 | 中国热带农业科学院环境与植物保护研究所 | Breeding device and method for experimental population of cassava single-claw mites |
| CN107432388B (en) * | 2017-07-24 | 2020-08-14 | 广西南亚热带农业科学研究所 | Cassava nutrient solution for efficiently propagating tetranychus cinnabarinus |
| CN108450417B (en) * | 2018-02-01 | 2021-02-26 | 河北省农林科学院植物保护研究所 | Application of absorbent cotton in collecting eggs of dichocrocis punctiferalis |
| CN109430593B (en) * | 2019-01-03 | 2022-02-08 | 华中农业大学 | Plant source feed for orius mirificus and preparation method thereof |
| CN109757438A (en) * | 2019-01-29 | 2019-05-17 | 河北省农林科学院植物保护研究所 | A kind of method of artificial feeding point bee coried |
| CN109644950B (en) * | 2019-02-11 | 2021-11-26 | 广东省烟草南雄科学研究所 | Large-scale breeding method for red-color lygus lucorum |
| CN110463666B (en) * | 2019-09-09 | 2021-10-01 | 浙江省农业科学院 | Indoor propagation method for orius minutus |
| CN113826587B (en) * | 2021-10-21 | 2023-03-17 | 广西壮族自治区农业科学院 | Indoor breeding method for adelphocoris suturalis |
| CN114271240A (en) * | 2022-01-14 | 2022-04-05 | 北京市农林科学院 | Breeding method of adelphocoris suturalis |
| CN115886163A (en) * | 2023-01-10 | 2023-04-04 | 河北省农林科学院植物保护研究所 | Artificial feed for orius sauteri and application of artificial feed |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101167533A (en) * | 2007-11-30 | 2008-04-30 | 河南省农业科学院植物保护研究所 | Bait for raising orius sauteri and method for group raising |
-
2008
- 2008-08-07 CN CN200810118204XA patent/CN101331868B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101167533A (en) * | 2007-11-30 | 2008-04-30 | 河南省农业科学院植物保护研究所 | Bait for raising orius sauteri and method for group raising |
Non-Patent Citations (2)
| Title |
|---|
| 张安盛等.东亚小花蝽若虫对西花蓟马成虫的捕食作用.《昆虫天敌》.2007,第29卷(第3期),108-112. * |
| 王广鹏等.小花蝽人工大量饲养研究进展.《昆虫天敌》.2005,第27卷(第2期),83-90. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101331868A (en) | 2008-12-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101331868B (en) | Artificial propagation method of minute pirate bugs | |
| CN106954592B (en) | Large-scale breeding method for Alliid lice spikemoss | |
| CN103262827B (en) | Method for feeding tea green leafhoppers indoors | |
| CN104585137B (en) | The method that field prevents and treats tobacco-plant pest-insect using fennel aphid breeding ladybug | |
| Brämick et al. | Testing of triploid tilapia (Oreochromis niloticus) under tropical pond conditions | |
| JP2007325541A (en) | Apparatus for breeding natural enemy insect and method for using the same | |
| CN103385220A (en) | Scale breeding method for encarsia formosa | |
| CN103155906A (en) | Aphidoletes aphidimyza mass reproduction technology and application technology | |
| CN109169131A (en) | A kind of garden landscape High-quality Cultivation method of water lily | |
| CN107980729A (en) | A kind of interior breeding mealybug and the method for Ban Shi encyrtids | |
| CN106900656B (en) | A kind of cigarette fleahopper industrial production method | |
| CN104026078B (en) | A kind of reticulitermes flavipe indoor intensive raising method | |
| CN109463238A (en) | A kind of breeding method of rhizoma polygonati stem tuber | |
| CN106489846A (en) | Diamondback moth artificial propagation production method and its application in biological control | |
| CN103766324A (en) | Method for biologically preventing green plant bugs by peristenus spretus in winter jujube orchard | |
| CN108935341B (en) | Method for raising cabbage butterfly velvet cocoon bee | |
| CN106386607A (en) | A graded multi-crop pond culture method for procambarus clarkii | |
| CN106900538A (en) | A kind of method that the onion production of hybrid seeds is carried out using fly | |
| CN112753658B (en) | Method for indoor breeding trichogramma dendrolimi by utilizing bombyx camphorata eggs | |
| CN107493972A (en) | A kind of pure artificial cultivation method of termite series collybia albuminosa | |
| CN204579614U (en) | The device that moth egg lures collection knitted by a kind of coconut | |
| CN107853058B (en) | Production method of young shoot with tea lesser leafhopper egg | |
| CN101960999B (en) | Method for preserving ophraella communa in winter and raising ophraella communa in plastic tent | |
| CN102771446A (en) | Propagation method of Diglyphus isaea by use of Chromatomyia horticola | |
| CN109197786B (en) | Method for breeding large amount of semi-closed curvicauda curvularia indoors |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110525 Termination date: 20170807 |