CN101167533A - Bait for raising orius sauteri and method for group raising - Google Patents
Bait for raising orius sauteri and method for group raising Download PDFInfo
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- CN101167533A CN101167533A CNA200710180585XA CN200710180585A CN101167533A CN 101167533 A CN101167533 A CN 101167533A CN A200710180585X A CNA200710180585X A CN A200710180585XA CN 200710180585 A CN200710180585 A CN 200710180585A CN 101167533 A CN101167533 A CN 101167533A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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Abstract
The invention relates to a feeding bait and process for colonially feeding Orius sauteries, the bait is made through a mode that flour mites are inoculated on a vegetal mixed base and is cultured and propagated, the environmental conditions of the inoculation, the cultivation and the propagation are that the relative humidity is 65-75 percent, the temperature is 24-26 DEG C, and the bait is cultured under the above environmental conditions for 15-25 days. The feeding method comprises hatch, the raising of nymphs, and the transfer of last instar nymphs or imagoes, the environmental conditions of feeding are that the temperature is 17.5-27.5 DEG C, the relative humidity is 60-90 percent, the photoperiodicity of the nymphs is 14-16 Liter : 10-8 days, during the stage of feeding the nymphs, the Orius sauteries are fed for 9-20 days by using the bait, and the quantity supplied of the bait of each Orius sauteri is 0.2-0.5 gram. Using the invention to cultivate the sauteries, the period of the growing development is short, the livability is high, the operation is simple, the practicability is strong, and the production cost is low. The spread and exploitation of the invention is beneficial to the non-polluted prevention of pests and the food safety.
Description
Technical field
The present invention relates to raising bait and the method for breeding of insect, the raising bait of particularly a kind of East Asia minute pirate bugs (O.sauteri) and group breeding method.
Background technology
Minute pirate bugs (Orius spp.) feeding habits are wide, can prey on some small insects and lepidopterous ovum such as bollworm, prodenia litura and small grubs etc. such as thrips, aphid, mite class, aleyrodid.Because characteristics such as it has a very wide distribution, adaptability is strong, quantity is many, predatism is strong are considered to the natural enemy insect that a class has the value utilized very much.
Owing to, cause the fulminant of some insects such as thrips, aphid, mite class and aleyrodid etc. to take place, and be difficult to control in recent years to the depending on unduly and unreasonable use of chemical pesticide.And minute pirate bugs has the better prevention effect to these small insects just, and Canada introduced America minute pirate bugs (O.insidiosus) first and successfully prevented and treated the Frankliniella occidentalis on the cucumber eighties in 20th century; Japanese subsequently scholar Kawai finds that also minute pirate bugs can be controlled at reduced levels for a long time with the Frankliniella occidentalis on the eggplant.Abroad the research of minute pirate bugs is used early, the now existing many companies of US and European carry out commercialization production to minute pirate bugs, mainly comprise America minute pirate bugs (O.majusculus) etc.Domesticly done big quantity research with regard to southern minute pirate bugs (O.similis), East Asia minute pirate bugs (O.sauteri), little minute pirate bugs (O.minutus) in recent years, but main contents concentrate on aspects such as biology, taxology and molecular biology, and explored the possibility of its artificial feeding, reproduction technique at present in enormous quantities does not see that as yet report is arranged.Wang Guangpeng etc. are at " the artificial mass rearing progress of minute pirate bugs " (insect natural enemy, the 27th the 2nd phase of volume of June in 2005) points out in the literary composition, the feed problem is important problem in the minute pirate bugs mass rearing, must seek not only cheaply but also easily to obtain, and the feed that suitable minute pirate bugs grows and breeds is to improve its survival rate and output; In addition, oviposition substrate and adult forage also are important problem in the minute pirate bugs mass rearing, and it directly affects the egg laying amount of female adult and the incubation rate of ovum, and then influence the adult pick-up rate of minute pirate bugs.
Summary of the invention
The technical problem to be solved in the present invention provides the raising bait of a kind of East Asia minute pirate bugs, and East Asia minute pirate bugs group's body method for breeding is provided.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
A kind of East Asia minute pirate bugs is raised bait, it is to make by the mode that inoculation flour mite on the vegetalitas mixed resin and cultivation are bred, wherein the inoculum concentration of flour mite is 25～50 a/gram vegetalitas mixed resin, the environmental condition of inoculation and cultivation breeding is: 24～26 ℃ of relative humidity 65%～75%, temperature, cultivated under above-mentioned environmental condition 15～25 days, flour mite in base-material amount reaches 500～1000/gram.
Described vegetalitas mixed resin by wheat bran, yeast and water with 8～10: 1: 1～2 weight ratio mixes.
Described vegetalitas compound by flour, rice with 3～5: 1 weight ratio mixes, and under 65%～75% relative humidity environment balance after 5～8 days.
Described flour mite is tyrophagus putrescentiae, thick pin mite, have a liking at least a in the squama mite.
A kind of East Asia minute pirate bugs group breeding method, its step comprises the transfer of ovum hatching, nymph raising, end nymph in age or adult, its feeding environment condition is: 17.5～27.5 ℃ of temperature, relative humidity 60%～90%, nymph and adult periodicity of illumination 14～16L: 10～8D, raise the stage nymph, raise bait with described East Asia minute pirate bugs and fed East Asia minute pirate bugs nymph 9～20 days, the quantity delivered that this stage is raised bait is 0.2～0.5 a gram/minute pirate bugs.
Can carry out nutrient in the described nymph stage of raising, can add speckling with the cotton balls that mass percent concentration is 2%～5% hydromel in the box raising of the East Asia minute pirate bugs nymph of feeding.
Raise the stage described nymph, can in nymph is raised box, be provided with and hide thing or overcover (as spitball and towel etc.) and carry out nymph and isolate, kill each other with the obstruction nymph.
Described East Asia minute pirate bugs group breeding method also comprises adult subculture step, with the melissa powder East Asia minute pirate bugs adult of feeding, and is its oviposition substrate with sterile-processed bright four seasons beanpod in this step.
The method that described bright four seasons beanpod is disinfected is that the Eusol with 1%～5% soaked bright four seasons beanpod after 8～15 minutes, then with behind the deionized water rinsing, dried or dry the moisture on beanpod surface.
The feeding environment condition of described East Asia minute pirate bugs is: 24～26 ℃ of temperature, relative humidity 60%～90%, periodicity of illumination 14～16L: 10～8D.
The basic process of East Asia minute pirate bugs group breeding and propagation is expressed as follows:
Adult gathers → raises → lay eggs on the kidney bean → change flour mite bait → ovum hatching → nymph on bait, to grow → transfer of raising 10 days → end nymph in age or adult in the use → incubator of quarantine measures: → a, transfer to new container breeding down with a melissa powder generation; Perhaps → and b, enter preparation and preserve test.
The present invention has actively useful effect:
1. lack the survival rate height growth cycle.In 17.5～27.5 ℃ scope, carry out adjustment, but proper extension or shorten the growth cycle (seeing Table 1) of East Asia minute pirate bugs, and then can regulate and control the growth cycle of East Asia minute pirate bugs as required easily.For example, be that raising adult from ovum needs 15 days under 25 ± 2 ℃ the condition in temperature, survival rate is up to about 70%.
2. easy and simple to handle, practical.The East Asia minute pirate bugs nymph of raising with bait of the present invention, survival rate height not only, and also easy and simple to handle; Can finish life cycle with the minute pirate bugs adult that the melissa powder is raised, the spawning rate height, single female egg laying amount reaches about 25, and higher survival rate is arranged; Kidney bean is as oviposition substrate, and raw material is cheap and easy to get, and the gained egg hatching rate is higher.This kind method is convenient to a large amount of breedings of carrying out minute pirate bugs indoor, and is convenient and practical, is beneficial to batch production production.
3. production cost is lower.The maximum cost that the East Asia minute pirate bugs is raised is labour cost, estimates to account for 70% of total cost.Illustrate below:
Per 1000 minute pirate bugs adults/aging nymph is raised rough cost
The operation of the adult bait and the vegetable material of laying eggs 60 minutes
Larva is raised the arrangement 20 minutes of container
Raise the cleaning 20 minutes of container
The separation of aging nymph-adult 100 minutes
The making of bait 10 minutes
Cost of labor adds up to 3.5 hours, 24.5 yuan/thousand (7 yuans/hour, the full-time servant in urban district, Zhengzhou); Total cost of production (adding the material energy) is 35 yuan/thousand, and the market price may reach 60～80 yuan/thousand.
4. the East Asia minute pirate bugs is as a kind of natural enemy insect; the predation scope is wide; can prey on multiple industrialized agriculture insect; can be used for the release of agricultural facility as aged nymph and first emergence adult; 1 every square metre; the release of crop initial bloom stage can be controlled the generation of aphid, red spider, thrips and aleyrodid for 1～2 time; as a kind of biological pesticide natural enemy insect product; can reduce chemical pesticide uses; promote agricultural product security and pollution-free food production, alleviate the development of pest resistance to insecticide, promote the sustainable control of insect; thereby the protection bio-diversity reduces environmental pollution.
The specific embodiment
1 one kinds of East Asia of embodiment minute pirate bugs is raised bait, with flour (cross 120 mesh sieves): the compound that rice=4: 1 (weight ratio) is formed is a base-material, with this mixed resin carry out disinfection the back and place in the drier that is added with the supersaturation saline solution, relative humidity balance with about 75% is after 7 days, on this base-material, inoculate flour mite, the inoculum concentration of flour mite is 25～50 a/gram base-material, behind the inoculation flour mite, drier places 25 ± 2 ℃ of insulating boxs to cultivate 20 days, when the flour mite in base-material amount reaches 500～1000/gram base-material, feed the bait of East Asia minute pirate bugs.
A kind of East Asia minute pirate bugs group breeding method, its step comprise the transfer of ovum hatching, nymph raising, end nymph in age or adult, and its feeding environment condition is: 25 ± 2 ℃ of temperature, relative humidity 75%, adult periodicity of illumination 14L: 10D.Raise the stage nymph, get the bait that contains flour mite and put into feed minute pirate bugs nymph 10～12 days of crisper, every 20g bait can guarantee to raise the whole nymph stage of 40 minute pirate bugs; (add speckling with the cotton balls that mass percent concentration is 3% hydromel in 20 * 15 * 15cm), supplement the nutrients and moisture, can improve spawning rate and egg laying amount at the crisper of minute pirate bugs nymph of feeding by the adult of its growth; And put into some spitballs or towel group in the box raising of East Asia minute pirate bugs nymph and carry out nymph and isolate as hiding thing, kill each other to hinder nymph, to improve the nymph survival rate.
As producing continuously, also need to carry out the adult subculture.In the adult subculture stage, with the melissa powder minute pirate bugs adult (injected volume of melissa powder is 0.2 a gram/minute pirate bugs) in Cans of feeding, single female laying eggs more than 20 can solve minute pirate bugs adult subculture problem, economical and convenient; And be its oviposition substrate with sterile-processed bright four seasons beanpod.Bright four seasons beanpod needs disinfection before use: the liquid lime chloride with 3% soaked bright four seasons beanpod after 10 minutes, then with deionized water rinsing clean after, and dry the subsidiary moisture in beanpod surface.East Asia minute pirate bugs adult can place 25 ± 2 ℃ of temperature, and relative humidity 75% is raised in the climate box of periodicity of illumination 14L: 10D.
2 one kinds of East Asia of embodiment minute pirate bugs is raised bait, flour mite is inoculated on the mixed resin of being made up of with 9: 1: 1 weight ratio wheat bran, yeast and water, then postvaccinal base-material is placed that relative humidity is 75%, under the environmental condition of 25 ± 1 ℃ of temperature, make the flour mite breeding, after 18 days when the flour mite in base-material amount reaches 500～1000/gram base-material, feed the bait of East Asia minute pirate bugs.
A kind of East Asia minute pirate bugs group breeding method, its step comprise the transfer of ovum hatching, nymph raising, end nymph in age or adult, and its feeding environment condition is: 25 ± 1 ℃ of temperature, relative humidity 70%, adult periodicity of illumination 16L: 8D.Raise the stage nymph, get the bait that contains flour mite and put into feed minute pirate bugs nymph 11～13 days of crisper, in the whole nymph stage, the quantity delivered of culturing feed is 0.2 a gram/minute pirate bugs; In the crisper of minute pirate bugs nymph of feeding, add the cotton balls that speckles with 5% hydromel; And put into towel group etc. in the box raising of minute pirate bugs nymph and hide thing and carry out nymph and isolate, kill each other to hinder nymph; In the adult subculture stage, with the melissa powder minute pirate bugs adult (melissa powder injected volume is 0.5 gram/head) in Cans of feeding; And be its oviposition substrate with sterile-processed bright four seasons beanpod.The processing method of kidney bean sterilization, sterilization is that the liquid lime chloride with 5% soaked kidney bean after 8 minutes, then with behind the deionized water rinsing, and dried up the subsidiary moisture in its surface.East Asia minute pirate bugs adult places 25 ± 1 ℃ of temperature, and relative humidity 70% is raised in the climate box of illumination 16L: 8D.
3 one kinds of East Asia of embodiment minute pirate bugs is raised bait, with flour (cross 100 mesh sieves): the compound that rice=5: 1 (weight ratio) is formed is a base-material, with carry out disinfection back and to place relative humidity be that balance under 75% the environmental condition is after 8 days of this mixed resin, on this base-material, inoculate flour mite, the inoculum concentration of flour mite is 30～50 a/gram base-material, behind the inoculation flour mite, drier places 25 ± 1 ℃ of insulating boxs to cultivate 15 days, when the flour mite in base-material amount reaches 500～800/gram base-material, feed the bait of East Asia minute pirate bugs.
A kind of East Asia minute pirate bugs group breeding method, its step comprise the transfer of ovum hatching, nymph raising, end nymph in age or adult, and its feeding environment condition is: 19 ± 1 ℃ of temperature, relative humidity 60%, adult periodicity of illumination 12L: 12D.Raise the stage nymph, get the bait that contains flour mite and put into feed minute pirate bugs nymph (the bait injected volume is 0.3 gram/head) 16～20 days of crisper; Add raising in the box of the minute pirate bugs nymph of feeding and to speckle with the cotton balls that mass percent concentration is 2% hydromel; And put into some spitballs in the box raising of East Asia minute pirate bugs nymph and carry out the nymph isolation as overcover.In the adult subculture stage, with the melissa powder minute pirate bugs adult (injected volume of melissa powder is 0.3 a gram/minute pirate bugs) in Cans of feeding; And be its oviposition substrate with sterile-processed bright four seasons beanpod.Bright four seasons beanpod needs disinfection before use: the liquid lime chloride with 1% soaked bright four seasons beanpod after 15 minutes, then with deionized water rinsing clean after, and dry the subsidiary moisture in beanpod surface.East Asia minute pirate bugs adult can place 19 ± 1 ℃ of temperature, and relative humidity 60% is raised in the climate box of periodicity of illumination 12L: 12D.
4 one kinds of East Asia of embodiment minute pirate bugs is raised bait, flour mite is inoculated on the mixed resin of being made up of with 9: 1: 1 weight ratio wheat bran, yeast and water, then postvaccinal base-material is placed that relative humidity is 80%, under the environmental condition of 25 ± 2 ℃ of temperature, make the flour mite breeding, after 25 days when the flour mite in base-material amount reaches 700～1000/gram base-material, feed the bait of East Asia minute pirate bugs.
A kind of East Asia minute pirate bugs group breeding method, its step comprise the transfer of ovum hatching, nymph raising, end nymph in age or adult, and its feeding environment condition is: 26 ± 1 ℃ of temperature, relative humidity 85%, adult periodicity of illumination 13L: 11D.Nymph is raised the stage, gets the bait that contains flour mite and puts into the crisper minute pirate bugs nymph of feeding, and in the whole nymph stage, the quantity delivered of culturing feed is 0.45 a gram/minute pirate bugs; In the crisper of minute pirate bugs nymph of feeding, add the cotton balls that speckles with 2% hydromel; And put into spitball etc. in the box raising of minute pirate bugs nymph and hide thing and carry out nymph and isolate.
5 one kinds of East Asia of embodiment minute pirate bugs is raised bait, with flour (cross 80 mesh sieves): the compound that rice=4: 1 (weight ratio) is formed is a base-material, this mixed resin is carried out disinfection the back with 70% relative humidity balance after 8 days, on this base-material, inoculate flour mite, the inoculum concentration of flour mite is 25～50 a/gram base-material, and behind the inoculation flour mite, drier places 25 ± 2 ℃ of insulating boxs to cultivate 18 days, when the flour mite in base-material amount reaches 700～900/gram base-material, feed the bait of East Asia minute pirate bugs.
A kind of East Asia minute pirate bugs group breeding method, its step comprise the transfer of ovum hatching, nymph raising, end nymph in age or adult, and its feeding environment condition is: 25 ℃ of temperature, relative humidity 78%, adult periodicity of illumination 13L: 11D.Raise the stage nymph, get the bait that contains flour mite and put into feed minute pirate bugs nymph 12 days of crisper, every 20g bait can guarantee to raise the whole nymph stage of 40 minute pirate bugs; (add in 20 * 15 * 15cm) and speckle with the cotton balls that mass percent concentration is 2% hydromel at the crisper of minute pirate bugs nymph of feeding; And put into some spitballs in the box raising of East Asia minute pirate bugs nymph and carry out the nymph isolation as hiding thing.
6 one kinds of East Asia of embodiment minute pirate bugs is raised bait, flour mite is inoculated on the mixed resin of being made up of with 9: 1: 2 weight ratio wheat bran, yeast and water, then postvaccinal base-material is placed that relative humidity is 70%, under the environmental condition of 25 ± 1 ℃ of temperature, make the flour mite breeding, after 18 days when the flour mite in base-material amount reaches 500～1000/gram base-material, feed the bait of East Asia minute pirate bugs.
A kind of East Asia minute pirate bugs group breeding method, its step comprise the transfer of ovum hatching, nymph raising, end nymph in age or adult, and its feeding environment condition is: 25 ± 3 ℃ of temperature, relative humidity 65%, adult periodicity of illumination 14L: 10D.Raise the stage nymph, get the bait that contains flour mite and put into feed minute pirate bugs nymph 10～15 days of crisper, in the whole nymph stage, the quantity delivered of culturing feed is 0.4 a gram/minute pirate bugs; In the crisper of minute pirate bugs nymph of feeding, add the cotton balls that speckles with 3% hydromel; And put into towel group etc. in the box raising of minute pirate bugs nymph and hide thing and carry out nymph and isolate.
Raise East Asia minute pirate bugs development duration and survival rate under table 1 condition of different temperatures
| Temperature | Development duration | Survival rate | |
| Ovum | 17.5℃ 22.5℃ 27.5℃ | 9.84a±0.93 5.28b±0.62 3.34c±0.27 | 0.89 0.56 0.54 |
| Nymph (flour mite raising) | 17.5℃ 22.5℃ 27.5℃ | 26.66a±1.88 20.81b±2.53 9.46c±0.86 | 0.88 0.68 0.73 |
Claims (10)
1. an East Asia minute pirate bugs is raised bait, it is characterized in that, it is to make by the mode that inoculation flour mite on the vegetalitas mixed resin and cultivation are bred, wherein the inoculum concentration of flour mite is 25～50 a/gram vegetalitas mixed resin, the environmental condition of inoculation and cultivation breeding is: 24～26 ℃ of relative humidity 65%～75%, temperature, under above-mentioned environmental condition, cultivated 15～25 days, when the amount of the flour mite in the base-material reaches 500～1000/gram.
2. East Asia according to claim 1 minute pirate bugs is raised bait, it is characterized in that, described vegetalitas mixed resin by wheat bran, yeast and water with 8～10: 1: 1～2 weight ratio mixes.
3. East Asia according to claim 1 minute pirate bugs is raised bait, it is characterized in that, described vegetalitas compound by flour, rice with 3～5: 1 weight ratio mixes, and under 65%～75% relative humidity environment balance after 5～8 days.
4. raise bait according to any described East Asia of the claim minute pirate bugs of claim 1 to 3, it is characterized in that, described flour mite is tyrophagus putrescentiae, thick pin mite, have a liking at least a in the squama mite.
5. East Asia minute pirate bugs group breeding method, its step comprises the transfer of ovum hatching, nymph raising, end nymph in age or adult, its feeding environment condition is: 17.5～27.5 ℃ of temperature, relative humidity 60%～90%, periodicity of illumination 14～16L: 10～8D, it is characterized in that, raise the stage nymph, raise bait with described East Asia minute pirate bugs and fed East Asia minute pirate bugs nymph 9～20 days, the quantity delivered that this stage is raised bait is 0.2～0.5 a gram/minute pirate bugs.
6. the group breeding method of East Asia according to claim 5 minute pirate bugs, it is characterized in that, carry out nutrient in the nymph raising stage, its method is to add raising in the container of the East Asia minute pirate bugs nymph of feeding to speckle with the cotton balls that mass percent concentration is 2%～5% hydromel.
7. the group breeding method of East Asia according to claim 6 minute pirate bugs is characterized in that, raises the stage nymph, is provided with to hide thing or overcover and carry out nymph and isolate in nymph is raised box, kills each other to hinder nymph.
8. according to the group breeding method of any described East Asia of the claim minute pirate bugs of claim 5～7, it is characterized in that, it also comprises adult subculture step, with the melissa powder East Asia minute pirate bugs adult of feeding, and is its oviposition substrate with sterile-processed bright four seasons beanpod in this step.
9. the group breeding method of East Asia according to claim 8 minute pirate bugs, it is characterized in that, the method that described bright four seasons beanpod is disinfected is, Eusol with 1%～5% soaked bright four seasons beanpod after 8～15 minutes, with behind the deionized water rinsing, dry or dry the moisture on beanpod surface then.
10. the group breeding method of East Asia according to claim 9 minute pirate bugs is characterized in that, the feeding environment condition of described East Asia minute pirate bugs adult is: 24～26 ℃ of temperature, relative humidity 60%～90%, periodicity of illumination 14～16L: 10～8D.
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| CN200710180585XA CN101167533B (en) | 2007-11-30 | 2007-11-30 | Bait for raising orius sauteri and method for group raising |
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| CN101167533B CN101167533B (en) | 2012-03-21 |
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| CN101331868B (en) * | 2008-08-07 | 2011-05-25 | 北京市农林科学院 | Artificial propagation method of minute pirate bugs |
| CN104222010A (en) * | 2013-06-21 | 2014-12-24 | 王伯明 | Method for artificial mass production of predatory miridae |
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| CN104938429A (en) * | 2015-06-30 | 2015-09-30 | 山东省农业科学院植物保护研究所 | Method for feeding orius sauteri |
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| CN101040612A (en) * | 2007-04-09 | 2007-09-26 | 张艳璇 | Production process of predacious mite |
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| CN101331868B (en) * | 2008-08-07 | 2011-05-25 | 北京市农林科学院 | Artificial propagation method of minute pirate bugs |
| CN104222006B8 (en) * | 2013-06-21 | 2016-12-21 | 中国农业科学院植物保护研究所 | A kind of artificial a large amount of methods producing minute pirate bugs |
| CN104222010A (en) * | 2013-06-21 | 2014-12-24 | 王伯明 | Method for artificial mass production of predatory miridae |
| CN104222006A (en) * | 2013-06-21 | 2014-12-24 | 王伯明 | Method for artificial mass production of orius |
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