CN104222010A - Method for artificial mass production of predatory miridae - Google Patents

Method for artificial mass production of predatory miridae Download PDF

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Publication number
CN104222010A
CN104222010A CN201310254277.2A CN201310254277A CN104222010A CN 104222010 A CN104222010 A CN 104222010A CN 201310254277 A CN201310254277 A CN 201310254277A CN 104222010 A CN104222010 A CN 104222010A
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fleahopper
predatism
host plant
prey
collection container
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CN104222010B (en
CN104222010B8 (en
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王伯明
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SHOUBONONG (BEIJING) BIOTECHNOLOGY CO., LTD.
Institute of Plant Protection Chinese Academy of Agricultural Sciences
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王伯明
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Abstract

The invention relates to a method for artificial mass production of predatory miridae. The method is characterized in that preys are bred by host plants and then the predatory miridae is bred by the bred preys. The method for artificial mass production of the predatory miridae has the advantages that not only are a predatory miridae prey breeding method and a predatory miridae breeding method provided, but also a predatory miridae collection method, a predatory miridae packaging method and a cultivating matrix treatment and reutilization method are provided; a complete production process is provided for commercialized production of the predatory miridae for the first time, large-scale mass breeding of the predatory miridae can be achieved, the predatory miridae in various insect activity states is packaged in a product produced by the method, and the product conforms to an overseas predatory miridae product packaging mode.

Description

A kind of method of artificial a large amount of production predatism fleahopper
Technical field
The present invention relates to a kind of method of artificial a large amount of production predatism fleahopper, belong to pest field of biological control.
Background technology
Predatism fleahopper refers in Miridae to have predacious natural enemy kind, and it can be used for the control of various agricultural insect.
Cigarette fleahopper (Nesidiocoris tenuis) and fleahopper (Macrolophus caliginosus) are external commercial important predatism fleahoppers, but all fail to realize merchandized handling China any one predatism fleahopper, it is also lack of standardization that this mainly commercially produces flow process because of relevant its, and its collection method does not also solve.Commercially produce utilization in order to realize predatism fleahopper class in China, relevant its is commercially produced flow process and is needed to study.
Summary of the invention
The object of this invention is to provide a kind of method of artificial a large amount of production predatism fleahopper, solve a large amount of production and the receipts problem of predatism fleahopper, a large amount of productions of predatism fleahopper can be realized.It is characterized in that first utilizing host plant to raise prey tetranychid, aphid, thrips, aleyrodid, liriomyza bryoniae, brown planthopper, recycle the method that the prey raised raises predatism fleahopper, described predatism fleahopper refers in Miridae to have predacious natural enemy kind, and concrete key operation step is:
1) raising of prey: launch in 0.5 day-15 days of first pair of true leaf host plant, prey provenance is inoculated on new host plant, wherein new host plant width of blade is at least at more than 1cm, the prey amount of average every strain host plant access is greater than 5, preferably more than 30, be do not cut or cut less to the terminal bud process of host plant, preferably only cut 1-2 time.Described prey refers to that various prey is raised separately or two kinds, two or more mixing raising.
2) raising of predatism fleahopper: the whole receptacle of raising predatism fleahopper is sealing, but can breathe freely, in the process of raising predatism fleahopper, without the need to dependent insect cage, without the need to isolation.
The raising method first of predatism fleahopper: access predatism fleahopper when every strain prey amount reaches more than 30; The access ratio of prey and predatism fleahopper is greater than 10: 1, is preferably greater than 50: 1, is less than 1000: 1; Be do not cut or cut less to the terminal bud process of host plant, preferably do not cut.
Next batch and later continuous method of raising predatism fleahopper, raising method can be identical with the raising method first of above-mentioned predatism fleahopper, but preferably the following stated raising method, concrete operations are: after last batch predatism fleahopper is gathered, new is a collection of with prey, the host plant of predatism fleahopper to be seeded is not continue to adopt inoculation predatism fleahopper to the method mass rearing predatism fleahopper on host plant, but after last batch predatism fleahopper is gathered within 10 days, preferably in 1 day, host plant to be seeded is moved in the receptacle of the complete predatism fleahopper of freshly harvested, be placed on raising frame, raise platform, ground, the host plant plantation utensil that the complete predatism fleahopper of freshly harvested is remaining, planting matrix or other can see that each of predatism fleahopper is local, the host plant plantation utensil that last batch stays is removed after 0.1 day-10 days, and then again put or shift the host plant stayed, determine whether directly to gather according to the amount of predatism fleahopper on host plant, or benefit fills out the host plant of part with prey again, or continue to raise, or benefit continues to raise after putting part predatism fleahopper again, but preferably no longer mend and put predatism fleahopper.Described plantation utensil refers to basin, dish, container etc. for planting host plant, is filled with planting matrix in these utensils, and host plant growth is in these planting matrixs.
3) collection method of predatism fleahopper: when the predatism fleahopper number of animals raised reaches the amount of gathering, just can gather to predatism fleahopper, collect, collection method cuts host plant with scissors, then by plants stems, branch puts into gathering unit together with leaf, also the blade of clip host plant or branches and leaves gathering unit can be put into separately, then gathering unit is sealed as early as possible, again gathering unit is placed in suitable space, namely the collection of each movable worm state of predatism fleahopper is can be used for after fixedly putting well, collection operation is every weekly check, collect more than 1 time, preferred every day checks, collect more than 1 time, till collecting pipe is collected less than predatism fleahopper, collecting pipe is that length is within 1m, for collecting the container of predatism fleahopper, guarantee to collect less than the plants stems can cleared up after predatism fleahopper in gathering unit, branch or leaf.
Described gathering unit refers to the utensil for collecting predatism fleahopper, gathering unit can be made up of two parts and also can be made up of three parts, preferred gathering unit is made up of three parts, if gathering unit is made up of two parts, comprise collection container and collecting pipe two parts, when collecting predatism fleahopper, directly and coated lycopene, to collecting pipe predatism fleahopper directly climb in collecting pipe in collection container; If gathering unit is made up of three parts, comprises collection container, be contained in collecting duct on collection container and collecting pipe three part, when collecting predatism fleahopper, collecting pipe is directly connected with collecting duct, and predatism fleahopper first climbs in collecting duct to climb in collecting pipe in collection container again.
Described gathering unit is placed on suitable space and refers to the space being designed for predatism fleahopper and collecting, require that this space has light unglazed when predatism fleahopper is collected, but need light when fixing gathering unit, inspection replacing collecting pipe, plants stems, branch or the leaf cleared up in gathering unit.
Described collection container refers to the part for loading predatism fleahopper and plants stems, branch or leaf, and collection container has 0.001m 3-1m 3volume, the manufactured materials of collection container must contain gauze, gauze, cloth, fly net, paper or other material can breathed freely and can effectively stop predatism fleahopper to escape, described gauze, gauze, cloth, fly net, paper or other can be breathed freely and the material that can effectively stop predatism fleahopper to escape can be used alone also that but two or more is used in combination, collection container also must have good sealing, can ensure predatism fleahopper except climbing to except in collecting pipe cannot climb out of from other place as far as possible, collection container also has the opening easily opened and seal, open opening and can load predatism fleahopper and plants stems, branch or leaf, seal opening and salable collection container.
Described collecting duct refers to and is contained on collection container for passage that predatism fleahopper climbs out of, predatism fleahopper can from collecting duct be contained in collection container that climb out of collection container, that connecting together from collecting duct and collecting pipe again crawls into collecting pipe, collecting duct number on collection container is one or more, and the length of collecting duct is within 1m.
4) packing method of predatism fleahopper: after utilizing gathering unit to collect predatism fleahopper, check the amount of predatism fleahopper and prey, guarantee not have prey or almost just can directly pack or first refrigerate in gameless situation wait to need in pack again, packing method is by packaging standard amount, each for predatism fleahopper worm state is put into vermiculite, wood chip, in the Packaging Bottle of wheat husk or husk, then bottle cap is covered tightly fast, Packaging Bottle body and bottle cap can seal completely, predatism fleahopper can be effectively stoped to escape, predatism fleahopper can apply or continue refrigeration after having packed, described Packaging Bottle bottle cap is with air-vent, air-vent size, shape is not fixed, but air-vent need by paper or gauze, gauze, cloth seals, preferred air-vent is sealed by paper.Described vermiculite, wood chip, wheat husk or husk refer to the package media of predatism fleahopper, and various package media is used alone, or two kinds and above used in combination.
5) process of planting matrix and re-using: first shift out used planting matrix in prey or predatism fleahopper receptacle, also receptacle can be shifted out together with used plantation utensil, after shifting out or pour out used planting matrix, place more than 0.1 day, directly sieve after used planting matrix certain mass dryness fraction to be achieved or smash rear continuation with cracker and use, if wherein sieve, screen sizes is 0.1cm-10cm, screen cloth can allow planting matrix to pass through and can prevent host plant root, stem, leaf or other foreign material pass through, the root, stem and leaf of host plant continues to use or do not recycle after can smashing in cracker, the planting matrix stayed after sieving or after smashing can directly utilize or used in combination or add other and be conducive to the composition that host plant grows with new planting matrix, but preferably through freezing before utilizing these used planting matrixs, high temperature deinsectization sterilizing or process of being exposed to the sun, to guarantee not having predatism fleahopper in planting matrix, prey or other insects, pathogenic bacteria etc.
The present invention provide not only the raising method of predatism fleahopper prey, the raising method of predatism fleahopper, but also provides the method for method and the packaging predatism fleahopper of collecting predatism fleahopper, and the process of planting matrix and recycling method.The present invention is first for predatism fleahopper commercially produces to provide a kind of complete production technology; application the present invention can not only realize the scale mass rearing of predatism fleahopper; and the packing of product of producing of application this method is each movable worm state of predatism fleahopper, meets the pack mode of external predatism fleahopper product.
Embodiment
Following methods is only the embodiment citing to claim and summary of the invention, does not represent the restriction to this claim and summary of the invention.
The raising of embodiment 1 prey: launch in 0.5 day-15 days of first pair of true leaf host plant, prey provenance is inoculated on new host plant, wherein new host plant width of blade is at least at more than 1cm, the prey amount of average every strain host plant access is greater than 5, preferably more than 30, be do not cut or cut less to the terminal bud process of host plant, preferably only cut 1-2 time.Described prey refers to that various prey is raised separately or two kinds, two or more mixing raising.
The raising of embodiment 2 predatism fleahopper: the whole receptacle of raising predatism fleahopper is sealing, but can breathe freely, in the process of raising predatism fleahopper, without the need to dependent insect cage, without the need to isolation.
The raising method first of predatism fleahopper: access predatism fleahopper when every strain prey amount reaches more than 30; The access ratio of prey and predatism fleahopper is greater than 10: 1, is preferably greater than 50: 1, is less than 1000: 1; Be do not cut or cut less to the terminal bud process of host plant, preferably do not cut.
Next batch and later continuous method of raising predatism fleahopper, raising method can be identical with the raising method first of above-mentioned predatism fleahopper, but preferably the following stated raising method, concrete operations are: after last batch predatism fleahopper is gathered, new is a collection of with prey, the host plant of predatism fleahopper to be seeded is not continue to adopt inoculation predatism fleahopper to the method mass rearing predatism fleahopper on host plant, but after last batch predatism fleahopper is gathered within 10 days, preferably in 1 day, host plant to be seeded is moved in the receptacle of the complete predatism fleahopper of freshly harvested, be placed on raising frame, raise platform, ground, the host plant plantation utensil that the complete predatism fleahopper of freshly harvested is remaining, planting matrix or other can see that each of predatism fleahopper is local, the host plant plantation utensil that last batch stays is removed after 0.1 day-10 days, and then again put or shift the host plant stayed, determine whether directly to gather according to the amount of predatism fleahopper on host plant, or benefit fills out the host plant of part with prey again, or continue to raise, or benefit continues to raise after putting part predatism fleahopper again, but preferably no longer mend and put predatism fleahopper.Described plantation utensil refers to basin, dish, container etc. for planting host plant, is filled with planting matrix in these utensils, and host plant growth is in these planting matrixs.
The collection method of embodiment 3 predatism fleahopper: when the predatism fleahopper number of animals raised reaches the amount of gathering, just can gather to predatism fleahopper, collect, collection method cuts host plant with scissors, then by plants stems, branch puts into gathering unit together with leaf, also the blade of clip host plant or branches and leaves gathering unit can be put into separately, then gathering unit is sealed as early as possible, again gathering unit is placed in suitable space, namely the collection of each movable worm state of predatism fleahopper is can be used for after fixedly putting well, collection operation is every weekly check, collect more than 1 time, preferred every day checks, collect more than 1 time, till collecting pipe is collected less than predatism fleahopper, collecting pipe is that length is within 1m, for collecting the container of predatism fleahopper, guarantee to collect less than the plants stems can cleared up after predatism fleahopper in gathering unit, branch or leaf.
Described gathering unit refers to the utensil for collecting predatism fleahopper, gathering unit can be made up of two parts and also can be made up of three parts, preferred gathering unit is made up of three parts, if gathering unit is made up of two parts, comprise collection container and collecting pipe two parts, when collecting predatism fleahopper, directly and coated lycopene, to collecting pipe predatism fleahopper directly climb in collecting pipe in collection container; If gathering unit is made up of three parts, comprises collection container, be contained in collecting duct on collection container and collecting pipe three part, when collecting predatism fleahopper, collecting pipe is directly connected with collecting duct, and predatism fleahopper first climbs in collecting duct to climb in collecting pipe in collection container again.
Described gathering unit is placed on suitable space and refers to the space being designed for predatism fleahopper and collecting, require that this space has light unglazed when predatism fleahopper is collected, but need light when fixing gathering unit, inspection replacing collecting pipe, plants stems, branch or the leaf cleared up in gathering unit.
Described collection container refers to the part for loading predatism fleahopper and plants stems, branch or leaf, and collection container has 0.001m 3-1m 3volume, the manufactured materials of collection container must contain gauze, gauze, cloth, fly net, paper or other material can breathed freely and can effectively stop predatism fleahopper to escape, described gauze, gauze, cloth, fly net, paper or other can be breathed freely and the material that can effectively stop predatism fleahopper to escape can be used alone also that but two or more is used in combination, collection container also must have good sealing, can ensure predatism fleahopper except climbing to except in collecting pipe cannot climb out of from other place as far as possible, collection container also has the opening easily opened and seal, open opening and can load predatism fleahopper and plants stems, branch or leaf, seal opening and salable collection container.
Described collecting duct refers to and is contained on collection container for passage that predatism fleahopper climbs out of, predatism fleahopper can from collecting duct be contained in collection container that climb out of collection container, that connecting together from collecting duct and collecting pipe again crawls into collecting pipe, collecting duct number on collection container is one or more, and the length of collecting duct is within 1m.
The packing method of embodiment 4 predatism fleahopper: after utilizing gathering unit to collect predatism fleahopper, check the amount of predatism fleahopper and prey, guarantee not have prey or almost just can directly pack or first refrigerate in gameless situation wait to need in pack again, packing method is by packaging standard amount, each for predatism fleahopper worm state is put into vermiculite, wood chip, in the Packaging Bottle of wheat husk or husk, then bottle cap is covered tightly fast, Packaging Bottle body and bottle cap can seal completely, predatism fleahopper can be effectively stoped to escape, predatism fleahopper can apply or continue refrigeration after having packed, described Packaging Bottle bottle cap is with air-vent, air-vent size, shape is not fixed, but air-vent need by paper or gauze, gauze, cloth seals, preferred air-vent is sealed by paper.Described vermiculite, wood chip, wheat husk or husk refer to the package media of predatism fleahopper, and various package media is used alone, or two kinds and above used in combination.
The process of embodiment 5 planting matrix and re-using: first in prey or predatism fleahopper receptacle, shift out used planting matrix, also receptacle can be shifted out together with used plantation utensil, after shifting out or pour out used planting matrix, place more than 0.1 day, directly sieve after used planting matrix certain mass dryness fraction to be achieved or smash rear continuation with cracker and use, if wherein sieve, screen sizes is 0.1cm-10cm, screen cloth can allow planting matrix to pass through and can prevent host plant root, stem, leaf or other foreign material pass through, the root, stem and leaf of host plant continues to use or do not recycle after can smashing in cracker, the planting matrix stayed after sieving or after smashing can directly utilize or used in combination or add other and be conducive to the composition that host plant grows with new planting matrix, but preferably through freezing before utilizing these used planting matrixs, high temperature deinsectization sterilizing or process of being exposed to the sun, to guarantee not having predatism fleahopper in planting matrix, prey or other insects, pathogenic bacteria etc.

Claims (8)

1. the method for an artificial a large amount of production predatism fleahopper, it is characterized in that first utilizing host plant to raise prey tetranychid, aphid, thrips, aleyrodid, liriomyza bryoniae, brown planthopper, recycle the method that the prey raised raises predatism fleahopper, described predatism fleahopper refers in Miridae to have predacious natural enemy kind, and concrete key operation step is:
1) raising of prey: launch in 0.5 day-15 days of first pair of true leaf host plant, prey provenance is inoculated on new host plant, wherein new host plant width of blade is at least at more than 1cm, the prey amount of average every strain host plant access is greater than 5, preferably more than 30, be do not cut or cut less to the item bud process of host plant, preferably only cut 1-2 time.
2) raising of predatism fleahopper: the whole receptacle of raising predatism fleahopper is sealing, but can breathe freely, in the process of raising predatism fleahopper, without the need to dependent insect cage, without the need to isolation.
The raising method first of predatism fleahopper: access predatism fleahopper when every strain prey amount reaches more than 30; The access ratio of prey and predatism fleahopper is greater than 10: 1, is preferably greater than 50: 1, is less than 1000: 1; Be do not cut or cut less to the terminal bud process of host plant, preferably do not cut.
Next batch and later continuous method of raising predatism fleahopper, raising method can be identical with the raising method first of above-mentioned predatism fleahopper, but preferably the following stated raising method, concrete operations are: after last batch predatism fleahopper is gathered, new is a collection of with prey, the host plant of predatism fleahopper to be seeded is not continue to adopt inoculation predatism fleahopper to the method mass rearing predatism fleahopper on host plant, but after last batch predatism fleahopper is gathered within 10 days, preferably in 1 day, host plant to be seeded is moved in the receptacle of the complete predatism fleahopper of freshly harvested, be placed on raising frame, raise platform, ground, the host plant plantation utensil that the complete predatism fleahopper of freshly harvested is remaining, planting matrix or other can see that each of predatism fleahopper is local, the host plant plantation utensil that last batch stays is removed after 0.1 day-10 days, and then again put or shift the host plant stayed, determine whether directly to gather according to the amount of predatism fleahopper on host plant, or benefit fills out the host plant of part with prey again, or continue to raise, or benefit continues to raise after putting part predatism fleahopper again, but preferably no longer mend and put predatism fleahopper.
3) collection method of predatism fleahopper: when the predatism fleahopper number of animals raised reaches the amount of gathering, just can gather to predatism fleahopper, collect, collection method cuts host plant with scissors, then by plants stems, branch puts into gathering unit together with leaf, also the blade of clip host plant or branches and leaves gathering unit can be put into separately, then gathering unit is sealed as early as possible, again gathering unit is placed in suitable space, namely the collection of each movable worm state of predatism fleahopper is can be used for after fixedly putting well, collection operation is every weekly check, collect more than 1 time, preferred every day checks, collect more than 1 time, till collecting pipe is collected less than predatism fleahopper, collecting pipe is that length is within 1m, for collecting the container of predatism fleahopper, guarantee to collect less than the plants stems can cleared up after predatism fleahopper in gathering unit, branch or leaf.
4) packing method of predatism fleahopper: after utilizing gathering unit to collect predatism fleahopper, check the amount of predatism fleahopper and prey, guarantee not have prey or almost just can directly pack or first refrigerate in gameless situation wait to need in pack again, packing method is by packaging standard amount, each for predatism fleahopper worm state is put into vermiculite, wood chip, in the Packaging Bottle of wheat husk or husk, then bottle cap is covered tightly fast, Packaging Bottle body and bottle cap can seal completely, predatism fleahopper can be effectively stoped to escape, predatism fleahopper can apply or continue refrigeration after having packed, described Packaging Bottle bottle cap is with air-vent, air-vent size, shape is not fixed, but air-vent need by paper or gauze, gauze, cloth seals, preferred air-vent is sealed by paper.
5) process of planting matrix and re-using: first shift out used planting matrix in prey or predatism fleahopper receptacle, also receptacle can be shifted out together with used plantation utensil, after shifting out or pour out used planting matrix, place more than 0.1 day, directly sieve after used planting matrix certain mass dryness fraction to be achieved or smash rear continuation with cracker and use, if wherein sieve, screen sizes is 0.1cm-10cm, screen cloth can allow planting matrix to pass through and can prevent host plant root, stem, leaf or other foreign material pass through, the root, stem and leaf of host plant continues to use or do not recycle after can smashing in cracker, the planting matrix stayed after sieving or after smashing can directly utilize or used in combination or add other and be conducive to the composition that host plant grows with new planting matrix, but preferably through freezing before utilizing these used planting matrixs, high temperature deinsectization sterilizing or process of being exposed to the sun, to guarantee not having predatism fleahopper in planting matrix, prey or other insects, pathogenic bacteria etc.
2. the method for a kind of artificial a large amount of production predatism fleahopper according to claim 1, is characterized in that described prey refers to that various prey is raised separately or two kinds, two or more mixing raising.
3. the method for a kind of artificial a large amount of production predatism fleahopper according to claim 1, it is characterized in that described plantation utensil refers to basin, dish, container etc. for planting host plant, be filled with planting matrix in these utensils, host plant growth is in these planting matrixs.
4. the method for a kind of artificial a large amount of production predatism fleahopper according to claim 1, it is characterized in that described gathering unit refers to the utensil for collecting predatism fleahopper, gathering unit can be made up of two parts and also can be made up of three parts, preferred gathering unit is made up of three parts, if gathering unit is made up of two parts, comprise collection container and collecting pipe two parts, when collecting predatism fleahopper, directly and coated lycopene, to collecting pipe predatism fleahopper directly climb in collecting pipe in collection container; If gathering unit is made up of three parts, comprises collection container, be contained in collecting duct on collection container and collecting pipe three part, when collecting predatism fleahopper, collecting pipe is directly connected with collecting duct, and predatism fleahopper first climbs in collecting duct to climb in collecting pipe in collection container again.
5. the method for a kind of artificial a large amount of production predatism fleahopper according to claim 1, it is characterized in that described gathering unit is placed on suitable space and refers to the space being designed for predatism fleahopper and collecting, require that this space has light unglazed when predatism fleahopper is collected, but need light when fixing gathering unit, inspection replacing collecting pipe, plants stems, branch or the leaf cleared up in gathering unit.
6. the method for a kind of artificial a large amount of production predatism fleahopper according to claim 1, it is characterized in that described vermiculite, wood chip, wheat husk or husk refer to the package media of predatism fleahopper, various package media is used alone, or two kinds and above used in combination.
7. the method for a kind of artificial a large amount of production predatism fleahopper according to claim 4, it is characterized in that described collection container refers to the part for loading predatism fleahopper and plants stems, branch or leaf, collection container has 0.001m 3-1m 3volume, the manufactured materials of collection container must contain gauze, gauze, cloth, fly net, paper or other material can breathed freely and can effectively stop predatism fleahopper to escape, described gauze, gauze, cloth, fly net, paper or other can be breathed freely and the material that can effectively stop predatism fleahopper to escape can be used alone also that but two or more is used in combination, collection container also must have good sealing, can ensure predatism fleahopper except climbing to except in collecting pipe cannot climb out of from other place as far as possible, collection container also has the opening easily opened and seal, open opening and can load predatism fleahopper and plants stems, branch or leaf, seal opening and salable collection container.
8. the method for a kind of artificial a large amount of production predatism fleahopper according to claim 4, it is characterized in that described collecting duct refers to be contained on collection container for passage that predatism fleahopper climbs out of, predatism fleahopper can from collecting duct be contained in collection container that climb out of collection container, that connecting together from collecting duct and collecting pipe again crawls into collecting pipe, collecting duct number on collection container is one or more, and the length of collecting duct is within 1m.
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