CN104782575B - Method for collecting and feeding tobacco thrips - Google Patents
Method for collecting and feeding tobacco thrips Download PDFInfo
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- CN104782575B CN104782575B CN201410752447.4A CN201410752447A CN104782575B CN 104782575 B CN104782575 B CN 104782575B CN 201410752447 A CN201410752447 A CN 201410752447A CN 104782575 B CN104782575 B CN 104782575B
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- onion thrips
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Abstract
The invention discloses a method for collecting and feeding tobacco thrips. The method comprises the following steps:1, collecting the tobacco thrips in the fields; 2, feeding the tobacco thrips indoors; 3, conducting bottle expansion and breeding to the tobacco thrips. The collecting method is simple and practicable, and the collection quantity and completeness can be guaranteed; according to the feeding method, a large-batch stable tobacco thrip population which is consistent in growth can be established, by adopting the collecting method and the feeding method, large-batch tobacco thrips which are consistent in growth can be obtained for meeting test demands, by means of the method, the influence of seasons to the tobacco thrips is overcome, and the limitation that the body of a single tobacco thrip is small, the tobacco thrips run fast and are not easy to capture and not strong in vitality, and adult tobacco thrips have hiding capacity are overcome, so that the problem that the large-batch tobacco thrips which are consistent in growth and used for meeting the test demands are difficult to collect in the fields is solved; the method has important meanings to launch correlational research of the tobacco thrips.
Description
Technical field
The present invention relates to insect collection and breeding technology, are specifically related to collection and the method for breeding of onion thrips.
Background technology
Onion thrips (Thrips tabaci) are under the jurisdiction of Thysanoptera (Thysanoptera) Ji also known as onion thrips, cotton thrips etc.
Equine (Thripidae) thrips subfamily (Thripinae) Thrips (Thrips), is the alliums such as Herba Alii fistulosi, Bulbus Allii and Bulbus Allii Cepae
And a kind of important insect of other crops.With International Agricultural Trade in recent years increasingly frequently, onion thrips gradually into
For important worldwide agricultural pests, serious economic loss is brought to agricultural production.In the Bulbus Allii Cepae planting site that New York is main
Area, if onion thrips can not be controlled, the yield of Bulbus Allii Cepae can reduce by 34%, or even 50%.In Canada, effectively prevent due to lacking
Measure is controlled, onion thrips cause the Canadian Bulbus Allii Cepae underproduction 43%.In Australia, nineteen forty-six onion thrips great outburst causes Bulbus Allii Cepae to subtract
Produce 75%.At home, onion thrips in 1999 are broken out in Shaanxi Province's Shangzhou City, and the Bulbus Allii for having more than 30 hectares is caused harm by onion thrips, quilt
Strain rate is done harm to up to 100%.2002, Yunnan Province Binchuan County onion thrips were caused harm up to 200 hectares of chive, cause the underproduction 150-200
Ton.Chemical prevention is the main method for nowadays preventing and treating onion thrips, but widely using with insecticide, and onion thrips are to more
Plant insecticide and generate Drug resistance.Drug-fast generation causes peasant inevitably original to prevent by increasing formulation rate and reach
Effect is controlled, so as to cause increasing for pesticide residues and environmental pollution.Therefore, research further to onion thrips is extremely urgent.
Chemistry and the screening of biological pesticide preparation and standardization measure are with to be required to large batch of, development in resistance mechanism research consistent
Onion thrips adult is for examination.Due to being difficult to catch by season and onion thrips individuality be little, motion is fast, vitality strong, adult tool
Have and hide the restriction such as ability, it is difficult to onion thrips large batch of, that development is consistent be collected for needed for test in field；Small insects
Collection and raising be Ecological Investigation, understand the technical method that insecticide and ecological Studies must be grasped.But, up till now for
Only, both at home and abroad the indoor feeding technology of onion thrips is rarely reported.Therefore the breeding technology of onion thrips is studied, onion thrips are realized
On a large scale, for a long time, continuously rearing is the important content for carrying out its integrated control technique research.
The content of the invention
It is an object of the invention to provide a kind of collection of onion thrips and method for breeding, can obtain high-volume using the method
, develop consistent onion thrips for needed for test.The method of the present invention overcomes impact of the season to onion thrips, and overcomes
Onion thrips individuality is little, motion fast and be difficult to catch, strong, adult has and hides the restriction such as ability vitality, it is difficult to adopt in field
Collect large batch of, consistent onion thrips are developed for the problem needed for test.
The purpose of the present invention is achieved through the following technical solutions：
Material requested and equipment：Box-shaped rice bucket, 200 mesh gauzes, filter paper, pest sucking device, foster worm bottle, rubber band, paper extraction, pocket knife,
Shears, needlework, fresh purple cabbage etc..
The collection of onion thrips and the concrete steps of method for breeding：
1st, the collection of field onion thrips
Box-shaped rice bucket is placed in high-pressure sterilizing pot and sterilizes, and the gauze of 200 mesh, filter paper, fresh Caulis et Folium Brassicae capitatae are placed on uviol lamp
Lower sterilization, pocket knife, shears, needlework will be through sterilizings, in the bottom layer overlay white filter paper of box-shaped rice bucket, will be new with pocket knife
Fresh purple cabbage blade is cut into square along main lobe arteries and veins, about places 7-10, take preparation on each box-shaped rice bucket filter paper
Good above-mentioned instrument and disposable glove, choose suitable host plant, gently pat host plant with handss, make host plant stem
Onion thrips on leaf are gone on the purple cabbage blade in box-shaped rice bucket, and repeatedly, collection is completed, and are spread in the bung of box-shaped rice bucket
The gauze of upper 200 mesh the opening inner cap that closes the lid, keep the air circulation in box-shaped rice bucket.
2nd, the raising of indoor onion thrips
Foster worm bottle is placed in high-pressure sterilizing pot and sterilizes, and paper extraction and filter paper are first dried in drying baker, then by itself and it is fresh
Purple cabbage leaf block sterilize under uviol lamp, shears, pocket knife, needlework will also sterilize, and spread three layers of paper extraction in the bottom of foster worm bottle,
Two filter paper are then covered with paper extraction, fresh purple cabbage is cut into the moon with pocket knife by the way of superposition intersects by filter paper
Its one end is worn by the bulk of tooth shape, about 80g or so per block with needlework, about stays the line of 10cm, in bottle mouth sleeve one
Rubber band, the one end for the line that then will be left behind are tied up to, transfer, will gather the life of returning with pest sucking device
The strong onion thrips of power are transferred to from box-shaped rice bucket in foster worm bottle and raise.The lid after repacking is covered, bottle is tiltedly placed on foster worm
On indoor foster worm frame, bottleneck controls temperature against where ventilation, in insectary for 25 DEG C, and relative humidity is 60%, passes through
Transparent foster worm bottle timely observes the situation of the inside, adds fresh purple cabbage in time according to the degree that purple cabbage leaf block is done
Leaf block, removes mouldy or has gone bad, and changes the paper extraction and filter paper of bottom in foster worm bottle in time, it is ensured that clear in foster worm bottle
It is clean.
3rd, the expansion bottle of onion thrips and breeding
Treat that the onion thrips in foster worm bottle reach certain quantity or four pieces of purple cabbage leaf blocks all inadequate its of offer take food
When can just expand bottle, two pieces of purple cabbage leaf blocks in bottle are transferred in new foster worm bottle, in original foster worm bottle and newly foster worm
Bottle in all add two pieces of new purple cabbage leaf blocks, it is specifically intended that Temperature and Humidity Control improperly in the case of, purple it is sweet
LANYE block occurs rotten, rotten, it is also possible to the infection pathogen during bottle is changed.So, aseptic ring to be close to
Operate under border.
Described box-shaped rice bucket is 19cm × 27cm × 14.7cm specifications, is transparent plastics material, and top cover has individual inner cap
The sharp 2177 box-shaped rice bucket of gold, solves the problems, such as that onion thrips individuality is little, moves soon and be difficult to catch, and box-shaped rice bucket space is greatly, thoroughly
Bright plastic material can be easy to the quantity of onion thrips in inspection box.
Filter paper of the present invention is equal with the bottom area of box-shaped rice bucket, can not only play moisture absorption and keep box-shaped rice bucket
The effect of interior drying, its white background are easy to the feature for observing onion thrips.
The present invention can be taken food for onion thrips using fresh plump purple cabbage blade, it is to avoid which is escaped or dead, newly
Fresh plump blade was difficult to be dried in the short time, it is ensured that do not thirst in the ovum short time that female adult worm is given birth to, while being easy to
Return to laboratory sub-bottle trematodiasiss.
Need to pat host plant stem and leaf in the acquisition method of the present invention, avoid as far as possible planting host when onion thrips are gathered
Thing is damaged；In addition, onion thrips adult is active, kind jump can be flown, beating gently can be such that onion thrips adult runs to hide
Like in box-shaped rice bucket on the purple cabbage blade that takes food.
200 mesh gauze of the present invention is covered on rice bucket, then covers meter bung again, opens little inner cap, not only
The atmosphere in the tub circulation of holding box-shaped rice is played, and is avoided that onion thrips escape.
Paper extraction and filter paper that drum head of the present invention is placed, are layered on the bottom of foster worm bottle by the way of superposition intersects when placement
Portion, can moisture absorption, and the place pupated is provided for onion thrips.
The present invention foster worm bottle be 1400ml, the transparency cover sugar bowl of bottleneck diameter 9cm, high 16cm, be easy to observe bottle in
Situation, the apparent growth promoter situation for observing onion thrips.
The bottle cap of the present invention is that, through repacking, acrylic material is changed into by the acrylic material gone in the middle of transparent cover
200 mesh gauzes, and gauze is fixed with PUR, gap should not be left, prevents onion thrips from escaping where gap.
Pest sucking device of the present invention is reequiped with Small-sized suction cleaner, adds a sebific duct in the end of vacuum cleaner,
The end of sebific duct links the liquid-transfering gun pipette tips of a 1cm again.Pest sucking device can selective vigorous onion thrips of life drain power,
It is difficult onion thrips is come to harm and is saved manpower.
The present invention is fast from fresh cabbage leaves, is to like causing harm in shelter due to onion thrips, and fresh purple cabbage
Block can provide food and hidden place for onion thrips, and fresh purple cabbage block can keep a very long time will not
It is dry, should not rot, go bad, place and there will not be abnormal flavour for a period of time.Fresh purple cabbage leaf block hanging can be kept into purple sweet
LANYE block is dried, and is difficult to make the onion thrips larva of bottom and pupa be weighed wounded or be killed by purple cabbage leaf block when stirring.
Four pieces of 80g purple cabbage leaf blocks of the present invention can take up the space of the 1/2 of foster worm bottle, on the one hand to onion thrips
Enough space lives are left, and on the other hand sufficiently substantial amounts of onion thrips are raised for needed for test using foster worm bottle.
Specific embodiment
Embodiment 1：
First wild onion thrips are gathered in field, box-shaped rice bucket is placed in high-pressure sterilizing pot and is sterilized, by the length of 200 mesh × wide
It is placed under uviol lamp for the gauze of 20cm × 30cm, filter paper, fresh Caulis et Folium Brassicae capitatae and sterilizes, it is long in the bottom layer overlay of box-shaped rice bucket
The white filter paper of × a width of 19cm × 27cm, and the fresh plumpness of 7-10 lengths of a film × a width of 5cm × 5cm is about placed on filter paper
Purple cabbage blade.Take ready above-mentioned instrument and disposable glove, with handss by the onion thrips on Herba Alii fistulosi stem and leaf gently
Pat in box-shaped rice bucket on purple cabbage blade, repeatedly, collection completes to spread 25cm × 35cm in the bung of box-shaped rice bucket,
The gauze of 200 mesh the opening inner cap that closes the lid, keep the air circulation in box-shaped rice bucket.
Returning laboratory carries out the foundation of population, foster worm bottle is placed in high-pressure sterilizing pot and is sterilized, and paper extraction and filter paper are first existed
Drying baker is dried, and then itself and fresh purple cabbage leaf block sterilizes under uviol lamp, spreads three layers in the bottom of foster worm bottle and takes out
Paper, is then covered with two filter paper on paper extraction, and fresh purple cabbage is cut into crescent moon by the way of superposition intersects by filter paper
Crescent shape purple cabbage leaf block one end is put on needlework, leaves the line of about 10cm, in bottle mouth sleeve by shape, about 80g or so per block
One rubber band, the linear system that will be left behind are hung in foster worm bottle on rubber band, and transfer, vitality collection returned with pest sucking device are strong
Onion thrips are transferred to from box-shaped rice bucket in foster worm bottle and raise.The lid after repacking is covered, it is 25 DEG C to be placed on temperature, relative humidity
Interior for 60% is raised, and through 3 breedings more than generation, sets up stable onion thrips sensitive population.
When needing to carry out biological test experiment.Purple cabbage leaf block in foster worm bottle is taken out and is placed in culture dish, strip off is purple
Cabbage leaves, picking onion thrips adult, drawing onion thrips adult with pest sucking device carries out bioassay test.
The above, the only present invention preferably specific embodiment, but protection scope of the present invention is not limited thereto,
Any those familiar with the art the invention discloses technical scope in, the change or replacement that can be readily occurred in,
Should all be included within the scope of the present invention.Therefore, protection scope of the present invention should be with the protection model of claims
Enclose and be defined.
Claims (7)
1. a kind of collection of onion thrips and method for breeding, it is characterised in that including three below step：
A) collection of field onion thrips：Box-shaped rice bucket is placed in high-pressure sterilizing pot and sterilizes, by gauze, filter paper, fresh purple cabbage
It is placed under uviol lamp and sterilizes, pocket knife, shears, needlework also pass through sterilizing, in the bottom layer overlay white filter paper of box-shaped rice bucket, uses
Fresh purple cabbage blade is cut into square along main lobe arteries and veins by pocket knife, places 7-10 pieces, choose on each box-shaped rice bucket filter paper
Suitable host plant, gently pats host plant with handss, the onion thrips on host plant stem and leaf is gone in box-shaped rice bucket
On purple cabbage blade, repeatedly, collection is completed, and spreads gauze and the opening inner cap that closes the lid in the bung of box-shaped rice bucket, is protected
Hold the air circulation in box-shaped rice bucket；
B) raising of indoor onion thrips：Foster worm bottle is placed in high-pressure sterilizing pot and sterilizes, and paper extraction and filter paper are first dried in drying baker,
Then itself and fresh purple cabbage leaf block are sterilized under uviol lamp, shears, pocket knife, needlework also sterilize, the bottom paving of foster worm bottle
Upper three layers of paper extraction, is then covered with two filter paper on paper extraction, and filter paper, will be fresh purple sweet with pocket knife by the way of superposition intersects
Indigo plant is cut into the bulk of crescent shape, every piece of 80g or so, its one end is worn with needlework, stays the line of 10cm, in bottle mouth sleeve one
Rubber band, the one end for the line that then will be left behind are tied up to, transfer, will gather the life of returning with pest sucking device
The strong onion thrips of power are transferred to from box-shaped rice bucket in foster worm bottle and raise；The lid after repacking is covered, bottle is tiltedly placed on foster worm
On indoor foster worm frame, bottleneck controls temperature against where ventilation, in insectary for 25 DEG C, and relative humidity is 60%, passes through
Transparent foster worm bottle timely observes the situation of the inside, adds fresh purple cabbage in time according to the degree that purple cabbage leaf block is done
Leaf block, it is ensured that the cleaning in foster worm bottle；
C) the expansion bottle of onion thrips and breeding；Treat that the onion thrips in foster worm bottle reach four pieces of purple cabbage of certain quantity or offer
Leaf block can just expand bottle when all not enough which takes food, and two pieces of purple cabbage leaf blocks in bottle are transferred in new foster worm bottle, original
Two pieces of new purple cabbage leaf blocks are all added in foster worm bottle and in new foster worm bottle, will be operated in the case where aseptic environment is close to.
2. a kind of collection of onion thrips according to claim 1 and method for breeding, it is characterised in that described box-shaped rice bucket
It is 19cm × 27cm × 14.7cm specifications, is transparent plastics material, top cover has the golden sharp 2177 box-shaped rice bucket of individual inner cap.
3. a kind of collection of onion thrips according to claim 1 and 2 and method for breeding, it is characterised in that described filter paper with
The bottom area of box-shaped rice bucket is equal.
4. a kind of collection of onion thrips according to claim 1 and method for breeding, it is characterised in that described is new light violet sweet
LANYE piece is plump.
5. a kind of collection of onion thrips according to claim 1 and 2 and method for breeding, it is characterised in that described foster worm bottle
Volume is 1400ml, bottleneck diameter 9cm, high 16cm, has transparency cover.
6. a kind of collection of onion thrips according to claim 1 and method for breeding, it is characterised in that described pest sucking device is
With Small-sized suction cleaner repacking, a sebific duct is added in the end of vacuum cleaner, reconnect the liquid relief of a 1cm in the end of sebific duct
Rifle pipette tips.
7. a kind of collection of the onion thrips according to claim 1 or 6 and method for breeding, it is characterised in that described gauze is
200 mesh.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410752447.4A CN104782575B (en) | 2014-12-09 | 2014-12-09 | Method for collecting and feeding tobacco thrips |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410752447.4A CN104782575B (en) | 2014-12-09 | 2014-12-09 | Method for collecting and feeding tobacco thrips |
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| CN104782575A CN104782575A (en) | 2015-07-22 |
| CN104782575B true CN104782575B (en) | 2017-04-26 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105393993A (en) * | 2015-12-28 | 2016-03-16 | 北京市农林科学院 | Method for artificial mass-reproduction of Echinothrips americanus Morgan |
| CN108849756B (en) * | 2018-06-20 | 2021-01-26 | 贵阳学院 | Breeding environment control method for thrips insects |
| CN108887246B (en) * | 2018-09-04 | 2023-12-12 | 云南农业大学 | Multifunctional device and method suitable for integration of frankliniella occidentalis collection, feeding and long-distance transportation |
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| CN2865276Y (en) * | 2005-12-22 | 2007-02-07 | 云南农业大学 | Thripid insects indoor feeding pot |
| CN101731183A (en) * | 2008-11-27 | 2010-06-16 | 甘肃农业大学 | Odontothrips loti feeding method |
| CN102524188B (en) * | 2012-01-20 | 2013-07-24 | 福建省农业科学院植物保护研究所 | Insect breeding bottom and breeding method of gynaikothrips ficorum |
| CN203233912U (en) * | 2012-12-26 | 2013-10-16 | 北京市农林科学院 | Device for collection and subpackage of thrips testing insects |
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