CN109984098B - Propagation method for experimental population of oriental fruit moth - Google Patents
Propagation method for experimental population of oriental fruit moth Download PDFInfo
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- CN109984098B CN109984098B CN201910400433.9A CN201910400433A CN109984098B CN 109984098 B CN109984098 B CN 109984098B CN 201910400433 A CN201910400433 A CN 201910400433A CN 109984098 B CN109984098 B CN 109984098B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention discloses a propagation method of a grapholitha molesta experimental population. Inoculating small adult pearIn a transparent insect-breeding tank, adding parchment paper in the tank to collect small pear eggs, soaking the parchment paper with eggs in formaldehyde for sterilization, rinsing with distilled water, cutting off the eggs from the parchment paper after blow-drying, placing the cut eggs into a breeding small pipe, and then placing the breeding small pipe into the breeding small pipe with the length of about 3cm3The artificial feed is characterized in that a small breeding pipe filled with eggs is placed into a glass tank, gauze is covered on the glass tank and then sealed, the glass tank is placed into an incubator for breeding, pupas on the gauze are collected at the later stage, the pupas are placed into a plastic box until emergence, and the emerged imagoes are connected into an insect breeding tank I, so that a complete period is formed. The small pear propagation method disclosed by the invention is simple, can effectively save manpower, and can meet the requirement of each insect-state pear in a laboratory.
Description
Technical Field
The invention relates to a method for breeding fruit tree pests, in particular to a method for breeding borer oriental fruit moth.
Background
Grapholita molesta (Busck), abbreviated as' Pyricularia ", is an important worldwide fruit tree pest, and is distributed in other areas except Tibet areas in China. The larva is mainly used for damaging young tips and fruits of rosaceous fruit trees such as peaches, pears, apples and the like, and huge loss is caused to the fruit tree planting and fruit trade every year.
The characteristics of the small pear such as biology, ethology and the like are researched indoors, and the method has important significance for finally preventing and controlling the small pear in the field. With the continuous and deep development of small pear prevention and control technologies, a large number of insect tests are required in the application research works such as biological prevention and control effect evaluation, novel pesticide effect determination, pest drug resistance determination and the like. Based on this, it is very important to develop research work on pear growth to obtain a large number of well-developed test insects indoors.
Related reports about artificial feeding methods for small pears at home and abroad are provided, the artificial feeding methods for small pears tend to be perfect in the aspects of illumination, photoperiod, temperature, humidity, artificial feed and the like, but the artificial feeding methods for small pears are lack of feeding devices and modes, so that the work of feeding small pears is extremely complex and time-consuming. In view of this, the invention improves and perfects the feeding scheme and the feeding vessel, so as to solve the problems of complicated and time-consuming small feeding of the pears and low adult harvest rate.
Disclosure of Invention
The invention aims to provide a propagation method for experimental population of oriental fruit moth, which is simple, time-saving and labor-saving and has high adult yield.
The technical scheme provided by the invention is as follows:
a propagation method for experimental population of oriental fruit moth comprises the following steps:
(1) adult breeding: feeding the small adult pears into a transparent insect breeding tank according to the female-male ratio of about 1:1, adding 5-20% of honey water every day, placing parchment paper egg paper on the tank bottom and tank wall for small pear spawning, and placing fresh apples into the tank to induce the small adult pears to spawn; the total amount of the small adult pear males and females is 300-600;
(2) and (3) replacement of the egg paper: replacing the egg paper and the apples in the insect breeding tank every 1-3 days; firstly, taking out the apples in the tank, and then horizontally inclining the insect breeding tank to replace the egg paper at the bottom of the tank; then vertically placing the insect breeding tank, shaking off adults rested on the egg paper on the tank wall, and taking out the egg paper; finally, new egg paper and apples are placed along the wall of the can from the can opening and sealed by gauze;
(3) and (3) processing the egg paper: in order to facilitate sterilization, the egg paper is taken out, and then the scales of the imagoes adhered to the egg paper are blown off by using the ear sucking ball. Soaking the egg paper with the scales blown off in a formaldehyde solution, rinsing for 1-3 times by using distilled water after soaking is finished, then clamping the egg paper by using forceps, and drying the egg paper in a blowing mode by using a blower to finish the sterilization work of the eggs. According to the density degree of small pear eggs on the egg paper, cutting the processed egg paper into egg slices with the size of about 0.2-0.5 cm2 for later split feeding; in this step, the hands of the operator and the instruments used need to be disinfected with 75% alcohol to reduce the risk of contamination of the egg paper;
(4) larva breeding: and (3) filling the prepared egg slices into the bottom of the feeding tubule, then filling the prepared artificial feed into the position, which is about 0.5-1.5 cm away from the egg paper, of the tube by using tweezers, and covering a tube cover. The feeding small tube II filled with the egg slices and the feed is placed into the glass tank, and the mouth of the feeding small tube faces the wall of the glass tank, so that the collection of the small pupae of the pears in the later period is facilitated. After the feeding tubules are placed, two layers of gauze are laid on the surfaces of the feeding tubules, then the mouth of the glass pot is sealed by the two layers of gauze and a rubber band, and finally the whole glass pot is placed in a constant temperature incubator; in the step, hands of operators and instruments used by the operators need to be disinfected by 75% alcohol so as to reduce the risk of pear larva stage feed mildew;
(5) collecting pupae: taking out the glass jar placed in the incubator for 15-18 days, cutting off the rubber band, taking off the gauze, opening the jar, taking out the gauze and the small worm breeding tube in the jar, taking down the jar opening, the jar bottom and the pupas on the gauze by using tweezers, placing all the pupas in the plastic box until eclosion occurs, finally cleaning the residual feed in the small worm breeding tube, and drying and sterilizing the residual feed for later use;
(6) adult supplementation: observing the pupa containing plastic box once every morning, and inoculating the eclosion imagoes in the box into an insect breeding tank to form virtuous circle.
According to the propagation method for the experimental population of the oriental fruit moth, the diameter of the bottom of the transparent insect breeding tank is 20-30 cm, the diameter of the opening of the transparent insect breeding tank is 10-20 cm, and the height of the transparent insect breeding tank is 15-40 cm.
According to the propagation method for the experimental population of the oriental fruit moth, the length of parchment paper at the bottom of a tank is 25-40 cm, and the width of the parchment paper is 25-40 cm; the parchment paper placed on the tank wall is 20-30 cm long and 10-25 cm wide.
According to the propagation method of the experimental population of the oriental fruit moth, the egg paper needs to be replaced within 1-3 days, so that the incubation of the oriental fruit moth is avoided, and honey water needs to be supplemented every day.
According to the propagation method of the experimental population of the oriental fruit moth, the egg paper needs to be soaked in 2-15% of formaldehyde for 3-10 minutes.
According to the propagation method of the experimental population of the oriental fruit moth, the volume of a feeding tubule is 5-20 ml, and a round hole with the diameter of 0.1-0.3 cm is formed in the position of a tubular cover.
According to the experimental population propagation method for the oriental fruit moth, the number of eggs put into each feeding small tube is 10-40.
According to the propagation method of the experimental population of the oriental fruit moth, artificial feed of about 1cm3 should be provided for every 10 eggs in a feeding small tube.
According to the propagation method of the experimental population of the oriental fruit moth, the feeding tubules are horizontally placed in the glass tank, and the mouths of the feeding tubules face the wall of the glass tank.
According to the experimental population propagation method for the oriental fruit moth, 30-200 feeding tubules are placed in each glass tank.
The experimental population propagation method for oriental fruit moth disclosed by the invention has the following advantages:
(1) the feeding small pipe is prepared by utilizing the boring property of the small pear larva, the small feeding pipe filled with the egg slices and the feed is horizontally placed into a glass tank, the mouth of the small feeding pipe faces the wall of the glass tank, and the mature larva can climb out of the small feeding pipe and then can directly climb along the wall of the glass tank to pupate. The size of the opening of the feeding small tube cover not only meets the drilled hole diameter of the mature larva, but also is enough to ensure the humidity of the feed during the growth period of the small pear larva.
(2) In the conventional insect breeding process, a container used for breeding insects is sealed by absorbent cotton, pupae are required to be stripped from feed and the absorbent cotton one by one after pear is small and pupated, and the part of work is complicated and time-consuming. The scheme simplifies the feeding scheme, enables the aged larvae to climb out of the insect-breeding small pipe by self, pupates on the opening of the glass tank, the bottom of the tank and the gauze, removes the link of stripping pupae in the feed and the absorbent cotton, and avoids the damage of stripping pupae to the insect body.
(3) The specific measures for disinfecting the small pear eggs are determined, the hatching rate of the eggs is guaranteed, the feed mildew in the feeding process is reduced, the health of the insects is guaranteed, meanwhile, the egg paper is directly placed into the larva feeding device, and the damage and the loss to the insects when the larvae are initially hatched through artificial inoculation are avoided.
Drawings
FIG. 1 is a schematic view of an insect tank of the present invention;
FIG. 2 is a schematic view of the insect-feeding tubule of the present invention.
Detailed Description
The present invention will be described in detail with reference to specific examples.
(1) Adult breeding: feeding small adult pears in a transparent insect breeding tank according to the male-female ratio of about 1:1 (total amount is 300), putting apples into the tank, adding 5% of honey water every day, placing parchment paper at the bottom and tank wall of the tank for small pear spawning, wherein the length and width of the parchment paper at the bottom of the tank are 25 cm; the length of the tank wall parchment paper is 20cm, and the width of the tank wall parchment paper is 15 cm; the mouth of the pot is sealed by gauze. The diameter of the insect-breeding tank body is 20cm, the diameter of the tank opening is 10cm, the height of the tank body is 30cm, and the height of the tank opening is 5 cm.
(2) And (3) replacement of the egg paper: the egg paper and the apples in the insect breeding tank are replaced every three days. In the morning, the apples in the tank are taken out, and then the insect breeding tank is horizontally inclined so as to replace the egg paper at the bottom of the tank; then vertically placing the insect breeding tank, shaking off adults statically laid on the egg paper, and taking out the egg paper; and finally, putting new egg paper and apples along the can wall from the can opening, and sealing the can opening by using gauze.
(3) And (3) processing the egg paper: after the egg paper is taken out, the scales of the imagoes adhered to the egg paper are blown off by using the ear sucking ball. Soaking the egg paper with the scales blown off in 5% formaldehyde solution for 5 minutes, rinsing with distilled water for 2 times after soaking, then clamping the egg paper with tweezers, and drying the egg paper in a natural wind mode of a blower to finish sterilization and disinfection of the eggs. Cutting sterilized egg paper into about 0.5cm according to density of small pear eggs on the egg paper2And (5) egg slices with the sizes, so that the eggs can be subpackaged and fed at the later stage. In this step, the operator and the instruments used need to be disinfected with 75% alcohol. The hatchability of eggs treated by the sterilization is greater than 90%.
(4) Larva breeding: filling the prepared egg slices into the bottom of a feeding tubule, wherein each tubule keeps 20 eggs; the feeding tubule is a test tube with a cover, the diameter of the test tube is 1.5cm, the height of the test tube is 9cm), and a round hole with the diameter of 0.3cm is formed in the position of the tube cover. Then using tweezers to make the prepared artificial feed about 3cm3The small tube is inserted into the breeding small tube about 1cm away from the egg paper, and the tube cover is covered. Feeding with egg pieces and feedThe small tubes are horizontally placed in a glass tank, the glass tank is like an insect breeding tank, but in order to facilitate the concentrated pupation of the aged larvae, the diameter of the tank opening of the glass tank is 6cm, and the height of the tank opening is 10 cm. The mouth of the small feeding pipe faces the wall of the glass tank, and 100 small feeding pipes can be placed in each glass tank. After the feeding tubules are placed, two layers of gauze are laid on the surfaces of the feeding tubules, then the openings of the glass jars are sealed by the two layers of gauze and rubber bands, finally the whole glass jar is placed into a constant-temperature incubator for feeding, the temperature of the incubator is 25 +/-1 ℃, the relative humidity is 60 +/-5%, the photoperiod is 15h:9h (L: D), and the light intensity is 500-1500 lux. In this step, the operator and the instruments used need to be disinfected with 75% alcohol. In the breeding process, the mildew rate of the breeding tubules is reduced by at least 70 percent through the disinfection treatment of eggs.
(5) Collecting pupae: taking out the glass jar placed in the incubator for 16 days, climbing pupae from the round hole of the small breeding tube by more than 95% of mature larvae, cutting off the rubber band, taking off the gauze, opening the jar, taking out the gauze and the small breeding tube in the jar, taking off the jar opening, the jar bottom and the pupae on the gauze by using tweezers, placing all the pupaes in the plastic box until the pupae is eclosized, finally cleaning the residual feed in the small breeding tube, drying and sterilizing for later use.
(6) Adult supplementation: observing the pupa containing plastic box once at 11:00 a.m. every day, inoculating the eclosion imagoes in the box into an insect breeding tank, and simultaneously adding 5% of honey water into the tank.
It will be understood that modifications and variations can be made by persons skilled in the art in light of the above teachings and all such modifications and variations are intended to be included within the scope of the invention as defined in the appended claims.
Claims (7)
1. A propagation method for experimental population of oriental fruit moth is characterized by comprising the following steps:
(1) adult breeding: feeding the small adult pears into a transparent insect breeding tank according to the female-male ratio of about 1:1, adding 5% -20% of honey water every day, placing parchment paper egg paper on the tank bottom and tank wall for small pear spawning, and placing fresh apples into the tank to induce the small adult pears to spawn; the total amount of the small adult pear males and females is 300-600;
(2) and (3) replacement of the egg paper: replacing the egg paper and the apples in the insect breeding tank every 1-3 days; firstly, taking out the apples in the tank, and then horizontally inclining the insect breeding tank to replace the egg paper at the bottom of the tank; then vertically placing the insect breeding tank, shaking off adults rested on the egg paper on the tank wall, and taking out the egg paper; finally, new egg paper and apples are placed along the wall of the can from the can opening and sealed by gauze;
(3) and (3) processing the egg paper: after the egg paper is taken out, blowing off the scales of the imagoes adhered to the egg paper by using an ear sucking ball; soaking the egg paper with scales blown off in a formaldehyde solution, rinsing for 1-3 times by using distilled water after soaking is finished, then clamping the egg paper by using a pair of tweezers, and drying the egg paper in a blowing mode by using a blower to finish sterilization of eggs; cutting the processed egg paper into about 0.2-0.5 cm according to the density of small pear eggs on the egg paper2Large and small egg pieces for later separate feeding; in this step, the hands of the operator and the instruments used need to be disinfected with 75% alcohol to reduce the risk of contamination of the egg paper;
(4) larva breeding: filling the prepared egg slices into the bottom of a feeding tubule, then filling the prepared artificial feed into the position, which is about 0.5-1.5 cm away from the egg paper, of the tube by using tweezers, and covering a tube cover; horizontally placing the small feeding pipes filled with the egg slices and the feed into a glass tank, wherein the mouths of the small feeding pipes face the wall of the glass tank, so that the collection of small pear pupae in the later period is facilitated; after the feeding tubules are placed, two layers of gauze are laid on the surfaces of the feeding tubules, then the mouth of the glass pot is sealed by the two layers of gauze and a rubber band, and finally the whole glass pot is placed in a constant temperature incubator; in the step, hands of operators and instruments used by the operators need to be disinfected by 75% alcohol so as to reduce the risk of pear larva stage feed mildew;
the volume of the feeding small tube is 5-20 ml, and a round hole with the diameter of 0.1-0.3 cm is formed in the tube cover;
the number of eggs put into each feeding small tube is 10-40;
(5) collecting pupae: taking out the glass jar placed in the incubator for 15-18 days, cutting off the rubber band, taking off the gauze, opening the jar, taking out the gauze and the small worm breeding tube in the jar, taking down the jar opening, the jar bottom and the pupas on the gauze by using tweezers, placing all the pupas in the plastic box until eclosion occurs, finally cleaning the residual feed in the small worm breeding tube, and drying and sterilizing the residual feed for later use;
(6) adult supplementation: observing the pupa containing plastic box once every morning, and inoculating the eclosion imagoes in the box into an insect breeding tank to form virtuous circle.
2. The propagation method of the experimental population of oriental fruit moth of claim 1, which is characterized in that: the bottom diameter of the transparent insect breeding tank is 20-30 cm, the diameter of the opening is 10-20 cm, and the height is 15-40 cm.
3. The propagation method of the experimental population of oriental fruit moth of claim 1, which is characterized in that: the length of the tank bottom parchment paper is 25-40 cm, and the width of the tank bottom parchment paper is 25-40 cm; the parchment paper placed on the tank wall is 20-30 cm long and 10-25 cm wide.
4. The propagation method of the experimental population of oriental fruit moth of claim 1, which is characterized in that: the egg paper needs to be replaced within 1-3 days so as to avoid hatching of small pear eggs, and honey water needs to be supplemented every day.
5. The propagation method of the experimental population of oriental fruit moth of claim 1, which is characterized in that: the egg paper needs to be soaked for 3-10 minutes by using 2-15% formaldehyde.
6. The propagation method of the experimental population of oriental fruit moth of claim 1, which is characterized in that: about 1cm should be provided for every 10 eggs in the feeding tubule3The artificial feed.
7. The propagation method of the experimental population of oriental fruit moth of claim 1, which is characterized in that: the number of the feeding tubules in each glass jar is 30-200.
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| CN101731186A (en) * | 2010-02-08 | 2010-06-16 | 西北农林科技大学 | Artificial rearing method of Grapholitha molesta Busck |
| CN105746440A (en) * | 2016-03-10 | 2016-07-13 | 山西省农业科学院植物保护研究所 | Artificial feeding device and artificial feeding method for oriental fruit moth |
| CN206101345U (en) * | 2016-09-17 | 2017-04-19 | 中国农业科学院果树研究所 | Small heart -eating peach worm adult is indoor to be raised and device of laying eggs |
| CN108849755A (en) * | 2018-06-10 | 2018-11-23 | 山西省农业科学院植物保护研究所 | Oriental fruit months larva store method |
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| CN101731186A (en) * | 2010-02-08 | 2010-06-16 | 西北农林科技大学 | Artificial rearing method of Grapholitha molesta Busck |
| CN105746440A (en) * | 2016-03-10 | 2016-07-13 | 山西省农业科学院植物保护研究所 | Artificial feeding device and artificial feeding method for oriental fruit moth |
| CN206101345U (en) * | 2016-09-17 | 2017-04-19 | 中国农业科学院果树研究所 | Small heart -eating peach worm adult is indoor to be raised and device of laying eggs |
| CN108849755A (en) * | 2018-06-10 | 2018-11-23 | 山西省农业科学院植物保护研究所 | Oriental fruit months larva store method |
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