CN106900656B - A kind of cigarette fleahopper industrial production method - Google Patents

A kind of cigarette fleahopper industrial production method Download PDF

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Publication number
CN106900656B
CN106900656B CN201710065933.2A CN201710065933A CN106900656B CN 106900656 B CN106900656 B CN 106900656B CN 201710065933 A CN201710065933 A CN 201710065933A CN 106900656 B CN106900656 B CN 106900656B
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Prior art keywords
cigarette fleahopper
raising
fleahopper
cigarette
semen viciae
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CN106900656A (en
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侯峥嵘
董民
李金萍
尹哲
郭喜红
孙贝贝
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Beijing plant protection station
China Agricultural University
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Beijing plant protection station
China Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics

Abstract

The invention discloses a kind of cigarette fleahopper industrial production methods, belong to the technical field of biological control of agricultural pests.The step of the method includes legume crop culture, the lifes of going out of rice moth egg to handle, the raising and collection of cigarette fleahopper.The industrial production method of cigarette fleahopper provided by the invention, it successfully solves plant source and adds a series of technical problems such as animal sources feed combinations, plant oviposition material, raising medium, changing cigarette fleahopper can only raise on a small scale in laboratory at present, be unable to satisfy the problem of prevention and treatment needs in actual production, substantially increase cigarette fleahopper the factorial production efficiency.Using cigarette fleahopper as predator large-area applications on vegetables, the access times and usage amount of insecticide can be greatly lowered, protect agroecological environment, improve foodsafety.

Description

A kind of cigarette fleahopper industrial production method
Technical field
The present invention relates to a kind of predator-cigarette fleahopper the factorial production technology, the biology for belonging to agricultural pests is anti- Control technical field.
Background technique
Greenhouse whitefly is distributed in a provinces and cities, China more than 20, is the primary pest on open country and greenhouse vegetable, especially Bemisia tabaci Wildness was caused disaster in recent years, seriously affected China's agricultural production.Trialeurodes vaporariorum (Trialeurodes vaporariorum) and tobacco powder Lice (Bemisia tabaci) host is extensive, and can cause harm more than 30 common greenhouse vegetables.It is wherein even more serious with Bemisia tabaci harm, Bemisia tabaci can also transmitted virus.Meanwhile greenhouse whitefly can also lead to sooty mould, seriously affect the photosynthesis of plant leaf blade.Often Rule prevention and treatment chemical pesticide it is long-term, be excessively used, cause aleyrodid that a variety of insecticides are produced with different degrees of resistance.It is single Take chemical agent to be prevented and treated to be difficult to prove effective, to have reduced the use of conventional pesticide, delay the generation of aleyrodid drug resistance, Especially want the research and development and application of Efforts To Develop biological prevention.Cigarette fleahopper is the Natural Enemies of Bemisia tabaci and Trialeurodes vaporariorum Westwood, Have the characteristics that prey ability is strong, adaptable, mobility is strong, the ovum or small to adult whitefly and nymph, lepidopterous insects The pests such as larva and tetranychid (Tetranychus cinnabarinus, Tetranychus urticae) have very strong predation.Aleyrodid is controlled using cigarette fleahopper The biological control means of class have the superiority that cannot compare of chemical prevention, the no pollution to the environment and lasting period is long.
During using the biological control of cigarette fleahopper pest control, how to obtain for a large amount of needed for field adaptability Cigarette fleahopper is the key factor for restricting cigarette fleahopper large-area applications.Predatism stinkbug is a class critically important in predator Group, has many advantages, such as that prey ability is strong, adaptable, mobility is strong, at present in most of Europe area, north African and the Middle East Cigarette fleahopper is produced by viable commercial product metaplasia in area, is widely used in preventing and treating the master such as Bemisia tabaci, trialeurodes vaporariorum, liriomyza bryoniae and tetranychid in greenhouse Want pest.The country is concentrated mainly on biological characteristics, prey ability, man-made feeds etc. for the research achievement of cigarette fleahopper, There are no artificial indoor rearing's technology of system, it is difficult to meet the actual needs of agricultural production.
Cigarette fleahopper is a kind of omnivorousness stinkbug, it is needed simultaneously, and feeding plant juice and aleyrodid, lepidopteran insect larvae etc. are small Type insect completes growth and development, and in food source plant and living body prey are indispensable.Meanwhile cigarette fleahopper breed when need by Ovum originates in fresh plant tissue, this is also that cigarette fleahopper carries out manually as a kind of omnivorousness natural enemy with huge applications potentiality The larger feature of captive breeding difficulty.External biological and ecological methods to prevent plant disease, pests, and erosion worker mostly uses Mediterranean flour moth and tomato branches and leaves to raise cigarette fleahopper, But Mediterranean flour moth feeding cost is higher, and is not distributed in China.Some scholars use beef liver, beef, sucrose solution, dimension life Artificial " larva " (not adding any plant component) that the mixtures such as element, egg yolk are made into raises fleahopper, in continuously 5 generations of raising It is later discovered that, nymph polypide is smaller, and development time is also long, is unable to reach factory farming level.
Summary of the invention
The present invention is a kind of as predatism stinkbug-cigarette fleahopper industrial production method, and the factory of cigarette fleahopper may be implemented Change breeding, meet aleyrodid in agricultural production, tetranychid, aphid, lepidoptera pest biological control demand.Cigarette provided by the invention is blind The industrial production method of stinkbug successfully solves plant source and adds animal sources feed combinations, plant oviposition material, raising medium etc. one Series technique problem, changing cigarette fleahopper can only raise on a small scale in laboratory at present, be unable to satisfy in actual production and prevent and treat need The problem of wanting, cigarette fleahopper the factorial production efficiency is substantially increased.Succeed in recent years using cigarette fleahopper as predator in north Large-area applications on the facilities vegetable in capital area considerably reduce the access times and usage amount of insecticide, protect agricultural Ecological environment improves foodsafety.
The present invention is using the rice moth egg of artificial breeding, legume crop tender stem etc. as the people of this omnivorousness natural enemy of cigarette fleahopper Work feed carries out batch production high-volume and breeds.
The industrial production method of cigarette fleahopper of the invention, the specific steps are as follows:
Step 1: carrying out legume crop culture.
Step 2: the raw processing of going out of rice moth egg.
Step 3: raising box prepares.The raising box has air hole, and is sealed using gauze.
Step 4: the raising and collection of cigarette fleahopper.
The present invention has the advantages that
(1) it overcomes in previous cigarette fleahopper room and is used as oviposition material cost height using tomato branches and leaves in breeding, be not easy to plant The problem of.The present invention utilizes the feature that beans is easily planted, growth cycle is short, and cigarette fleahopper egg laying amount and the production on tomato branches and leaves Ovum amount no significant difference, beans tender stem become the best vegetable material laid eggs for cigarette fleahopper.
(2) solve the problems, such as that animal fodder type is uncertain in previous cigarette fleahopper breeding, with foreign applications Mediterranean powder Phycitid (Ephestia kuhniella Zeller) ovum feeding cost height is compared, and present invention application rice moth egg raising, advantage embodies In rice moth in China's breeding technology height, feeding cost is low, and rice moth egg can be obtained largely, and rice moth egg processing technique is simple.
(3) the present invention changes production fleahopper class natural enemy in previous domestic patent of invention and can only raise on living body host plant It supports, the limitation that operation difficulty is big, output is uncertain, commercial insect difficulty is collected, devises distinctive breathable plastics raising box, Oviposition plant, animal fodder, medium and cigarette fleahopper kind worm are put in simultaneously in raising box, batch production, normalizing operation are conducive to, The raising and collection efficiency for greatly improving cigarette fleahopper realize the fleahopper class natural enemy the factorial production technology in China huge prominent It is broken.
Detailed description of the invention
Fig. 1 provides the industrial production method flow chart of cigarette fleahopper for the present invention.
Specific embodiment
The present invention is described in detail with reference to the accompanying drawings and examples.
The present invention provides a kind of industrial production method of cigarette fleahopper, process as shown in Figure 1, the specific steps are as follows:
Step 1: carrying out legume crop culture:
A, semen viciae fabae seed is placed in clear water under room temperature and is impregnated 24 hours, remove water after waiting semen viciae fabae seed bubble to rise, then Continuation vernalization is covered with wet towel, it is each primary during which to eluriate semen viciae fabae seed in the morning, afternoon and evening daily.
B, when bean sprout growth length is 0.5~1cm, bean sprouts is planted in turf: vermiculite: perlite=1:1:1 (volume Than) Nutrition Soil in, for Nutrition Soil with a thickness of 2~3cm, beans kind seeding row spacing is 2 × 2cm, is then irrigated with water.Equal semen viciae fabaes young plant is long It is stand-by to 20~30cm.
Step 2: the raw processing of going out of rice moth egg:
Fresh rice moth egg is placed under 40 watts of ultraviolet lamps and irradiates life of going out in 12 hours, is saved in 5 DEG C, the holding time is no more than 7 days.
Step 3: raising box prepares:
Happy button box of the plastics that the raising box standard being applied in this method for breeding is long 40cm* wide 25cm* high 40cm, Two walls of box make the air hole of 2 diameter 9cm respectively, are sealed with the gauze of 60 mesh.Box disinfects in alcohol rear spare.
Step 4: the raising and collection of cigarette fleahopper:
A, daily raising: in the vermiculite of raising cassette bottom portion 1~2cm of layer overlay thickness, fresh semen viciae fabae seedling 2~3 of clip Be placed on vermiculite, be sprinkled into 0.5ml go out it is raw after rice moth egg, cigarette fleahopper kind worm female male worm each 20 for accessing field acquisition cover Box top cover is raised, the growth cabinet or receptacle of Light To Dark Ratio 16:8,25 ± 1 DEG C of temperature, relative humidity 55%~65% are placed in Interior raising.Interval addition in 2~3 days fresh semen viciae fabae seedling 2-3 root (10~15cm long) and rice moth egg (0.5ml), being spaced 10 days will The bean seedlings of exsiccation remove.
B, expand numerous raising: after the cigarette fleahopper of daily raising is raised 20 days, cigarette fleahopper worm amount in box of raising increase by 500 with On, movable cigarette fleahopper on the semen viciae fabae seedling and semen viciae fabae seedling in original raising box can be moved into new raising box and be raised, in favor of Population amplification.Cigarette fleahopper in original raising box is normally raised.Added inside the described new raising box vermiculite, rice moth egg, Semen viciae fabae seedling, wherein 1~2cm of vermiculite is thick, and semen viciae fabae seedling 2~3, every 10~15cm long, the rice moth egg 0.5ml after life of going out.
C, cigarette fleahopper is collected: after cigarette fleahopper expands numerous raising 20 days in new raising box, worm amount can increase to 5 times in box, i.e., Collect packaging.
Cigarette fleahopper is put into medium-sized air blower together with vermiculite and is separated and is purified, by after purification cigarette fleahopper nymph and After adult carries out a series of inspections of indexs such as sex ration, survival rate, being put into is to carry out in the packing box of medium equipped with sawdust Become natural enemy insect product after the packaging of different worm states and quantity specifications, is used for agricultural production.
Embodiment 1: cigarette fleahopper different egg plant lays eggs to it the influence of situation
1. materials and methods:
1.1 worm sources: according to (25~29 DEG C of optimum rearing after field acquisition;RH:55~65%, L: D=16: 8) numerous The cigarette fleahopper educated.
1.2 methods: after cigarette fleahopper adult feeding rice moth egg and tobacco leaf that field acquisition is arrived, by F2 for first emerging adult 1 To in the Clear glass bottles and jars of access 20*10cm, feed is added, while being put into processing 1~3, is then sealed with transparent gauze and rubber band Bottleneck is closed, replaces feed and oviposition plant daily.Replaced oviposition plant is put into teat glass, observes nymph daily Hatch situation, 20 repetitions of every processing finally count every group of adult eclosion number.
Oviposition plant processing: it is shown in Table 1.
Table 1: Adult worms producting eggs plant variety
Serial number Processing
Processing 1 Tomato branches and leaves
Processing 2 Tobacco leaf branches and leaves
Processing 3 Beans tender stem
2. result such as table 2:
2 test result of table
By comparing to the cigarette fleahopper adult eclosion number under the raising of different egg material, beans tender stem and tomato are found Spray effect is better than tobacco leaf, and the next-generation cigarette fleahopper quantity highest that cigarette fleahopper obtains after laying eggs in beans tender stem can achieve 76.2.The oviposition effect of tomato spray and beans tender stem no significant difference, but use tomato spray as oviposition plant, cultivation at This is higher, is unfavorable for batch production mass rearing, therefore final determine uses beans tender stem (the semen viciae fabae seedling i.e. in the present invention) as cigarette The best oviposition plant of fleahopper.

Claims (5)

1. a kind of cigarette fleahopper industrial production method, it is characterised in that: specific step is as follows for the method,
Step 1: carrying out legume crop culture;Detailed process are as follows:
A, semen viciae fabae seed is placed in clear water under room temperature and is impregnated 24 hours, water is removed after waiting semen viciae fabae seed bubble to rise, then with wet Towel covers continuation vernalization, and it is each primary during which to eluriate semen viciae fabae seed in the morning, afternoon and evening daily;
B, when bean sprout growth length is 0.5~1cm, by bean sprouts plantation in Nutrition Soil, Nutrition Soil is with a thickness of 2~3cm, beans kind Seeding row spacing is 2 × 2cm, is then irrigated with water;It is stand-by that equal semen viciae fabaes young plant grows to 20~30cm;In the Nutrition Soil, according to body Product ratio has: turf: vermiculite: perlite=1:1:1;
Step 2: the raw processing of going out of rice moth egg;
Step 3: raising box prepares;The raising box has air hole, and is sealed using gauze;
Step 4: the raising and collection of cigarette fleahopper.
2. a kind of cigarette fleahopper industrial production method according to claim 1, it is characterised in that: go out described in step 2 Raw processing, which refers to for fresh rice moth egg to be placed under 40 watts of ultraviolet lamps, irradiates life of going out in 12 hours, saves in 5 DEG C.
3. a kind of cigarette fleahopper industrial production method according to claim 1, it is characterised in that: feeding described in step 4 It supports and collects specifically:
A, daily raising: in the vermiculite of raising cassette bottom portion 1~2cm of layer overlay thickness, 10~15cm long of clip fresh semen viciae fabae seedling 2 ~3 are placed on vermiculite, be sprinkled into 0.5ml go out it is raw after rice moth egg, cigarette fleahopper kind worm female male worm each 20 for accessing field acquisition, Raising box top cover is covered, Light To Dark Ratio 16:8,25 ± 1 DEG C of temperature, the growth cabinet of relative humidity 55%~65% or feeding are placed in Support interior raising;Interval addition in 2~3 days fresh semen viciae fabae seedling 2~3 and rice moth egg 0.5ml, are spaced 10 days bean seedlings by exsiccation It removes;
B, expand numerous raising: after the cigarette fleahopper of daily raising is raised 20 days, cigarette fleahopper worm amount increases by 500 above in raising box, will Movable cigarette fleahopper, which moves into new raising box, on semen viciae fabae seedling and semen viciae fabae seedling in original raising box raises;
C, cigarette fleahopper is collected: after cigarette fleahopper expands numerous raising 20 days in new raising box, worm amount can increase to 5 times in box, Ji Keshou Collection packaging.
4. a kind of cigarette fleahopper industrial production method according to claim 3, it is characterised in that: the new raising box Vermiculite, rice moth egg, semen viciae fabae seedling are added in the inside, wherein 1~2cm of vermiculite is thick, and semen viciae fabae seedling 2~3, every 10~15cm long goes out Rice moth egg 0.5ml after life.
5. a kind of cigarette fleahopper industrial production method according to claim 3, it is characterised in that: the cigarette fleahopper is collected Refer to for cigarette fleahopper to be put into medium-sized air blower together with vermiculite and separated and purified, by the cigarette fleahopper nymph and adult after purification Carry out sex ration, survival rate index inspection after, be put into equipped with sawdust be medium packing box in, carry out different worm state sum numbers The packaging of gauge lattice.
CN201710065933.2A 2017-02-06 2017-02-06 A kind of cigarette fleahopper industrial production method Active CN106900656B (en)

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CN111264473B (en) * 2020-02-18 2021-09-28 广西壮族自治区林业科学研究院 Method for breeding Exorista bigelovii by utilizing galleria mellonella
CN113439712A (en) * 2020-03-24 2021-09-28 北京市农林科学院 Large-scale breeding method of orius sauteri with simple operation
CN114271240A (en) * 2022-01-14 2022-04-05 北京市农林科学院 Breeding method of adelphocoris suturalis
CN115281157A (en) * 2022-08-12 2022-11-04 云南省烟草公司楚雄州公司 New application of hermetia illucens, food source of hermetia illucens Li Chun, artificial feeding method of hermetia illucens Li Chun and industrial production method of hermetia illucens

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CN104222006B8 (en) * 2013-06-21 2016-12-21 中国农业科学院植物保护研究所 A kind of artificial a large amount of methods producing minute pirate bugs
CN104938429B (en) * 2015-06-30 2017-04-05 山东省农业科学院植物保护研究所 A kind of method for raising Orius
CN105746435B (en) * 2016-02-25 2018-05-11 北京市农林科学院 A kind of method using rice moth egg breeding chrysopa septempunctata larva
CN106305628A (en) * 2016-08-22 2017-01-11 北京阔野田园生物技术有限公司 Method for scale propagation of amphiareus obscuriceps

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