CN106305628A - Method for scale propagation of amphiareus obscuriceps - Google Patents
Method for scale propagation of amphiareus obscuriceps Download PDFInfo
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- CN106305628A CN106305628A CN201610701412.7A CN201610701412A CN106305628A CN 106305628 A CN106305628 A CN 106305628A CN 201610701412 A CN201610701412 A CN 201610701412A CN 106305628 A CN106305628 A CN 106305628A
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- corsage
- stinkbug
- blackhead
- fork
- scale
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- 238000000034 method Methods 0.000 title claims abstract description 26
- 241001671706 Amphiareus obscuriceps Species 0.000 title abstract description 12
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims abstract description 21
- 235000011613 Pinus brutia Nutrition 0.000 claims abstract description 21
- 241000018646 Pinus brutia Species 0.000 claims abstract description 21
- 238000005286 illumination Methods 0.000 claims abstract description 21
- 235000013601 eggs Nutrition 0.000 claims abstract description 20
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 claims abstract description 14
- 241000753145 Sitotroga cerealella Species 0.000 claims abstract description 10
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229960004889 salicylic acid Drugs 0.000 claims abstract description 7
- 241000578422 Graphosoma lineatum Species 0.000 claims description 89
- 206010000496 acne Diseases 0.000 claims description 88
- 102000002322 Egg Proteins Human genes 0.000 claims description 37
- 108010000912 Egg Proteins Proteins 0.000 claims description 37
- 210000004681 ovum Anatomy 0.000 claims description 37
- 241000607479 Yersinia pestis Species 0.000 claims description 31
- 239000000758 substrate Substances 0.000 claims description 16
- 230000017448 oviposition Effects 0.000 claims description 15
- 241000819999 Nymphes Species 0.000 claims description 7
- 238000009395 breeding Methods 0.000 claims description 7
- 230000001488 breeding effect Effects 0.000 claims description 7
- 239000011295 pitch Substances 0.000 claims description 5
- 240000008669 Hedera helix Species 0.000 claims description 4
- 235000009161 Espostoa lanata Nutrition 0.000 claims description 3
- 240000001624 Espostoa lanata Species 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 241000270322 Lepidosauria Species 0.000 claims 1
- 238000011161 development Methods 0.000 abstract description 3
- 230000000644 propagated effect Effects 0.000 abstract 2
- 238000002791 soaking Methods 0.000 abstract 1
- 230000035899 viability Effects 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 19
- 230000008144 egg development Effects 0.000 description 9
- 241000238631 Hexapoda Species 0.000 description 8
- 230000013011 mating Effects 0.000 description 7
- 238000012360 testing method Methods 0.000 description 5
- 241001635552 Anthocoridae Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000258937 Hemiptera Species 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 230000003628 erosive effect Effects 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241001124076 Aphididae Species 0.000 description 1
- 244000060924 Brassica campestris Species 0.000 description 1
- 235000005637 Brassica campestris Nutrition 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241001635529 Orius Species 0.000 description 1
- 241001647846 Orius insidiosus Species 0.000 description 1
- 241001635528 Orius minutus Species 0.000 description 1
- 241001635523 Orius sauteri Species 0.000 description 1
- 241000320508 Pentatomidae Species 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- 241000083513 Punctum Species 0.000 description 1
- 241001414987 Strepsiptera Species 0.000 description 1
- 241001414989 Thysanoptera Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000000266 injurious effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 244000062645 predators Species 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention discloses a method for scale propagation of amphiareus obscuriceps. The method is characterized in that rice moth eggs are used as feed at the adult stage of the amphiareus obscuriceps; matsumura pine scale eggs subjected to soaking with salicylic acid are used as feed at the nymphal stage; the amphiareus obscuriceps are propagated by combining with improved temperature, humidity and illumination conditions. The method has the advantages that firstly, the development time of the amphiareus obscuriceps from eggs to adults is shortened, and the reproduction speed and efficiency of the amphiareus obscuriceps are improved; secondly, the development proportion of the amphiareus obscuriceps from the eggs to the adults is increased; thirdly, after ten generations of the amphiareus obscuriceps are continuously propagated by using the propagation method, the viability of the amphiareus obscuriceps is not weakened, and the population stability of the amphiareus obscuriceps is favorably kept.
Description
Technical field
The invention belongs to the technical field of biological control of agriculture and forestry insect, be specifically related to the mistake at Biological control agriculture and forestry injurious insect
The method for scaled breeding of blackhead fork corsage stinkbug Amphiareus obscuriceps (Poppius) used in journey.
Background technology
In forestry ecosystem, a large amount of use of chemical pesticide causes the Drug resistance of environmental pollution and insect to produce, to agricultural
Economic damage increases the weight of.Release predatism, parasitic insect are main biological and ecological methods to prevent plant disease, pests, and erosion natural enemy, can within a short period of time by Agroforestry ecosystem system
Target pest in system controls below economic injury level, reaches to reduce agricultural economic loss, the purpose of minimizing environmental pollution.
Increasing year by year of biological and ecological methods to prevent plant disease, pests, and erosion natural enemy insect demand, raising natural enemy insect with batch production vast propagation becomes the heat that scientific research person pays close attention to
Point.
Anthocoridae in Semiptera (Hemiptera) has multiple insect predator, such as Similis (Orius
Similis Zheng), Orius Orius sauteri (Poppius), Orius minutus Orius minuius
Linnaeus, the method for breeding of America minute pirate bugs Orius insidiosu, replacement feedstuff, oviposition substrate etc. are all scholar's research
Focus, but the blackhead of Anthocoridae fork corsage stinkbug Amphiareus obscuriceps (Poppius) as natural enemy insect
Purposes rarely has report, and its method for breeding and correlation technique do not have been reported that.
Blackhead fork corsage stinkbug, body length 2 3mm, yellowish-brown, have microtriche, gather punctum.Same body colour, crown dark brown is extremely
Black, compound eye is big, brownish red.Pronotary medial recess, also there is horizontal falling at escutcheon rear portion.Fore wing yellowish-brown, diaphragm is translucent,
Tool dark brown speckle.The same body colour of foot, the dark speckle of each leg section tool 2.Inhabit rick, dry branches and fallen leaves, predation aphid, scale insect, aleyrodid,
The small insects such as thrips, demodicid mite class, are distributed in Liaoning, the Inner Mongol, Beijing, Hebei, Shandong, Henan, Shaanxi, Jiangsu, Zhejiang, lake
South, Gansu, Sichuan, Guangxi, Yunnan, Hainan, Taiwan, Japan.The present invention passes through lot of experiments, finds that a kind of scale is numerous
The method educating blackhead fork corsage stinkbug, uses rice moth egg to use soaked as feedstuff, nymph in the blackhead fork corsage stinkbug adult stage
Salicylic Matsumura pine scale ovum is as feedstuff, and combines the temperature after improvement, humidity, illumination condition so that it is disclosure satisfy that scale,
Efficiently breed the needs of blackhead fork corsage stinkbug.
Summary of the invention
The method that the purpose of the present invention is to propose to a kind of propagation in scale blackhead fork corsage stinkbug, pitches corsage stinkbug adult at blackhead
Phase uses rice moth egg to use as feedstuff, nymph to soak salicylic Matsumura pine scale ovum as feedstuff, and after combining improvement
Temperature, humidity, illumination condition so that it is disclosure satisfy that scale, efficiently breed blackhead fork corsage stinkbug needs.
The technical scheme taked to achieve the object of the present invention is as follows:
A kind of method of propagation in scale blackhead fork corsage stinkbug, it is characterised in that: the method includes the steps of: obtains black
The ovum of head fork corsage stinkbug, breeds blackhead fork corsage stinkbug, preserves blackhead fork corsage stinkbug；
Preferably, the described ovum obtaining blackhead fork corsage stinkbug comprises the following steps: blackhead pitches corsage stinkbug is female, male imago
Put into and lay eggs in case, and in case of laying eggs, put into rice moth egg, oviposition substrate, change oviposition substrate every day；
Preferably, the described blackhead fork corsage stinkbug that breeds comprises the steps of
1) oviposition substrate is moved in box for feeding pests, and in box for feeding pests, put into Matsumura pine scale ovum as feedstuff；
2) box for feeding pests moving into greenhouse, the temperature in greenhouse is 27 DEG C-28 DEG C, and humidity is 75%-78%, and illumination condition is
Illumination in 14 hours, 10 hours dark；
3) blackhead fork corsage stinkbug, the quantity of Matsumura pine scale ovum in box for feeding pests is made regular check on, it is ensured that feedstuff is sufficient；
4) when nymph has more than 85% to sprout wings, blackhead fork corsage stinkbug adult is collected.
Preferably, described preservation blackhead fork corsage stinkbug comprises the following steps: the blackhead fork corsage stinkbug adult that will collect
Carrying out sex identification, female, male imago are separately moved in dactylethrae, dactylethrae opening cotton balls seals；
Preferably, described Matsumura pine scale ovum soaks the 15-30 second in the salicylic acid solution of 2-5 μ g/ml, after drying under room temperature
Use as feedstuff；
Preferably, described oviposition substrate is Seem Lablab Album angle；
The adult of the blackhead fork corsage stinkbug that the present invention breeds is for breeding the purposes of blackhead fork corsage stinkbug of future generation.
Present invention have the advantage that
1, the present invention is by using rice moth egg to use soaked water as feedstuff, nymph in the blackhead fork corsage stinkbug adult stage
The Matsumura pine scale ovum of poplar acid is as feedstuff, and combines the temperature after improvement, humidity, illumination condition, breeds the blackhead fork corsage obtained
Stinkbug shortened from the time of egg development to adult, improve reproduction speed and the efficiency of blackhead fork corsage stinkbug.
2, the mating system of the present invention improves the blackhead fork corsage stinkbug ratio from egg development to adult.
3, the mating system of the present invention is after breeding ten generation blackhead fork corsage stinkbugs continuously, and the vitality of blackhead fork corsage stinkbug does not has
Have and weaken, be conducive to keeping the population stability of blackhead fork corsage stinkbug.
Detailed description of the invention
Below in conjunction with embodiment to further instruction of the present invention, the percentage ratio in embodiment is weight ratio, but this
Bright it is not limited thereto.
Embodiment one
1, material:
1.1 for examination worm source: test blackhead used fork corsage stinkbug adult is that Yanqing County of Beijing Yeyahu collects；
2, test method: (test is in triplicate)
(1) blackhead pitches corsage stinkbug is female, male imago is put into and laid eggs in case, and the ratio of female male imago is 1:1；And at case of laying eggs
In put into rice moth egg, oviposition substrate (fresh Seem Lablab Album angle), every day changes oviposition substrate, in oviposition substrate ovum count, institute
Stating the transparent plastic case that case is 50cm*50cm*50cm of laying eggs, there is an opening casing upper end, muffles opening with 120 mesh gauzes and prevents
Blackhead is fled from by fork corsage stinkbug；
(2) 50 box for feeding pests are set, oviposition substrate are moved in box for feeding pests, it is ensured that the ovum quantity moved in each box for feeding pests
Identical, described box for feeding pests is the transparent plastic case of 60cm*60cm*60cm, and there is an opening casing upper end, muffles with 120 mesh gauzes
Opening prevents blackhead fork corsage stinkbug from fleeing from；Matsumura pine scale ovum is soaked 20 seconds in the salicylic acid solution of 3 μ g/ml, dries under room temperature
In rear input box for feeding pests；Box for feeding pests moves into greenhouse, and the temperature in greenhouse is 27 DEG C, and humidity is 78%, and illumination condition is 14 little
Shi Guangzhao, 10 hours dark；Make regular check on blackhead fork corsage stinkbug, the quantity of Matsumura pine scale ovum in box for feeding pests, it is ensured that feedstuff is sufficient；Black
The ovum of head fork corsage stinkbug is bred 16.3 days, and the egg development of blackhead fork corsage stinkbug is adult, collects blackhead fork corsage stinkbug adult；
(3) blackhead collected fork corsage stinkbug adult is carried out sex identification, female, male imago are separately moved into dactylethrae
In, dactylethrae opening cotton balls seals.
(4) controlled trial group:
Comparative example 1: move into the box for feeding pests 10 of identical ovum quantity in selecting step (2), puts into rice moth egg as feedstuff
Box for feeding pests；Box for feeding pests moves into greenhouse, and the temperature in greenhouse is 27 DEG C, and humidity is 78%, illumination condition be illumination in 14 hours, 10
Hour dark；Make regular check on blackhead fork corsage stinkbug, the quantity of Matsumura pine scale ovum in box for feeding pests, it is ensured that feedstuff is sufficient；Blackhead fork corsage
The ovum of stinkbug is bred 17.6 days, and the egg development of blackhead fork corsage stinkbug is adult, collects blackhead fork corsage stinkbug adult.
Comparative example 2: move into the box for feeding pests 10 of identical ovum quantity in selecting step (2), by Matsumura pine scale ovum 3 μ g/ml's
Salicylic acid solution soaks 20 seconds, puts in box for feeding pests after drying under room temperature；Box for feeding pests moves into greenhouse, and the temperature in greenhouse is
25 DEG C, humidity is 75%, and illumination condition is illumination in 16 hours, 8 hours dark；Make regular check on blackhead fork corsage stinkbug in box for feeding pests,
The quantity of Matsumura pine scale ovum, it is ensured that feedstuff is sufficient；The ovum of blackhead fork corsage stinkbug is bred 17.1 days, and the egg development of blackhead fork corsage stinkbug is
Adult, collects blackhead fork corsage stinkbug adult.
Comparative example 3: move into the box for feeding pests 10 of identical ovum quantity in selecting step (2), throws Matsumura pine scale ovum as feedstuff
Enter box for feeding pests；Box for feeding pests moves into greenhouse, and the temperature in greenhouse is 27 DEG C, and humidity is 78%, illumination condition be illumination in 14 hours,
10 hours dark；Make regular check on blackhead fork corsage stinkbug, the quantity of Matsumura pine scale ovum in box for feeding pests, it is ensured that feedstuff is sufficient；Blackhead fork breast
The ovum of flower stinkbug is bred 17.3 days, and the egg development of blackhead fork corsage stinkbug is adult, collects blackhead fork corsage stinkbug adult.
(5) blackhead fork corsage stinkbug development duration
Note: in table 1, the egg development of blackhead fork corsage stinkbug is the meansigma methods repeating test for three times to the time of adult
Data in table 1 show: the present invention is by using rice moth egg as feedstuff, nymph in the blackhead fork corsage stinkbug adult stage
Phase uses soaked salicylic Matsumura pine scale ovum as feedstuff, and combined the temperature after improvement, humidity, illumination condition, bred
To blackhead fork corsage stinkbug to be shorter than comparative example 1 (use single feed), comparative example 2 from the time of egg development to adult (conventional
Temperature, humidity, illumination condition), comparative example 3 (Matsumura pine scale ovum does not soaks salicylic acid), it is seen that Feed selection, temperature, humidity, illumination
Affect the time of breeding of blackhead fork corsage stinkbug etc. condition, and the mating system of the present invention is conducive to improving the numerous of blackhead fork corsage stinkbug
Grow speed and efficiency.
(6) the adult pick-up rate of blackhead fork corsage stinkbug under the conditions of difference is bred
Oviposition substrate Seem Lablab Album angle in comparative example 1 in step (2), step (4), comparative example 2, comparative example 3 is carried out mirror
Inspection, record oviposition substrate on ovum amount, the oviposition substrate having added up ovum amount is put into box for feeding pests, ovum amount controls at 150.
Note: in table 2, blackhead fork corsage stinkbug adult pick-up rate is the meansigma methods repeating test for three times
Data in table 2 show: the present invention is by using rice moth egg as feedstuff, nymph in the blackhead fork corsage stinkbug adult stage
Phase uses soaked salicylic Matsumura pine scale ovum as feedstuff, and combined the temperature after improvement, humidity, illumination condition, and blackhead is pitched
Corsage stinkbug from the ratio of egg development to adult more than comparative example 1 (use single feed), comparative example 2 (conventional temperature, humidity,
Illumination condition), comparative example 3 (Matsumura pine scale ovum does not soaks salicylic acid), it is seen that Feed selection, the impact of the condition such as temperature, humidity, illumination
The adult pick-up rate of blackhead fork corsage stinkbug, and the mating system of the present invention is conducive to the adult improving blackhead fork corsage stinkbug to obtain
Rate, the present invention greatly improves the reproductive efficiency of blackhead fork corsage stinkbug, reduces cost.
(7) pass on raising and blackhead is pitched the blodynamic comparison of corsage stinkbug
Bred for ten generations the most continuously according to the present invention, comparative example 1, comparative example 2, the mating system of comparative example 3, take first
Generation, blackhead each 50 of the corsage stinkbug female adult worm of fork in the tenth generation, survey its body length, body width with the micrometer in stereomicroscope
Amount, obtains the blackhead fork average body length of the female male imago of corsage stinkbug, average body width on Brassica campestris L.
Note: the blackhead fork body length of corsage stinkbug adult, the wide meansigma methods being all to repeat for three times test of body in table 3
Data in table 3 show: the mating system of the present invention is after continuous ten generations breed, and blackhead pitches the life of corsage stinkbug
Power does not weaken, and even strengthens, and individual change is big；And the worm of the blackhead fork corsage stinkbug that comparative example 1, comparative example 2, comparative example 3 are bred
Body diminishes, and vitality weakens, and the especially vitality of comparative example 1 weakens the most obvious.Visible, the mating system of the present invention is suitable
Propagation in scale blackhead fork corsage stinkbug, breeds how generation is also not result in that the vitality of blackhead fork corsage stinkbug weakens continuously.
Claims (7)
1. the method for a propagation in scale blackhead fork corsage stinkbug, it is characterised in that: the method includes the steps of: obtains blackhead
The ovum of fork corsage stinkbug, breeds blackhead fork corsage stinkbug, preserves blackhead fork corsage stinkbug.
The method of a kind of propagation in scale blackhead the most according to claim 1 fork corsage stinkbug, it is characterised in that: described obtains
The ovum taking blackhead fork corsage stinkbug comprises the following steps: blackhead pitches corsage stinkbug is female, male imago is put into and laid eggs in case, and at case of laying eggs
In put into rice moth egg, oviposition substrate, change oviposition substrate every day.
The method of a kind of propagation in scale blackhead the most according to claim 2 fork corsage stinkbug, it is characterised in that: described is numerous
Educate blackhead fork corsage stinkbug to comprise the steps of
1) oviposition substrate is moved in box for feeding pests, and in box for feeding pests, put into Matsumura pine scale ovum as feedstuff；
2) box for feeding pests moving into greenhouse, the temperature in greenhouse is 27 DEG C-28 DEG C, and humidity is 75%-78%, and illumination condition is 14 hours
Illumination, 10 hours dark；
3) blackhead fork corsage stinkbug, the quantity of Matsumura pine scale ovum in box for feeding pests is made regular check on, it is ensured that feedstuff is sufficient；
4) when nymph has more than 85% to sprout wings, blackhead fork corsage stinkbug adult is collected.
The method of a kind of propagation in scale blackhead the most according to claim 3 fork corsage stinkbug, it is characterised in that: described guarantor
Deposit blackhead fork corsage stinkbug to comprise the following steps: the blackhead collected fork corsage stinkbug adult is carried out sex identification, by female, male imago
Separately moving in dactylethrae, dactylethrae opening cotton balls seals.
The method of a kind of propagation in scale blackhead the most according to claim 3 fork corsage stinkbug, it is characterised in that: described pine
Dry a red-spotted lizard ovum soaks the 15-30 second in the salicylic acid solution of 2-5 μ g/ml, as feedstuff uses after drying under room temperature.
The method of a kind of propagation in scale blackhead the most according to claim 3 fork corsage stinkbug, it is characterised in that: described product
Ovum substrate is Seem Lablab Album angle.
The adult of blackhead the most according to claim 4 fork corsage stinkbug is for breeding the purposes of blackhead fork corsage stinkbug of future generation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610701412.7A CN106305628A (en) | 2016-08-22 | 2016-08-22 | Method for scale propagation of amphiareus obscuriceps |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610701412.7A CN106305628A (en) | 2016-08-22 | 2016-08-22 | Method for scale propagation of amphiareus obscuriceps |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106900656A (en) * | 2017-02-06 | 2017-06-30 | 北京市植物保护站 | A kind of cigarette fleahopper industrial production method |
| CN113439712A (en) * | 2020-03-24 | 2021-09-28 | 北京市农林科学院 | Large-scale breeding method of orius sauteri with simple operation |
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| CN101167533A (en) * | 2007-11-30 | 2008-04-30 | 河南省农业科学院植物保护研究所 | Bait for raising orius sauteri and method for group raising |
| CN102293182A (en) * | 2011-07-18 | 2011-12-28 | 山东省农业科学院植物保护研究所 | Orius sauteri raising method |
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| CN101167533A (en) * | 2007-11-30 | 2008-04-30 | 河南省农业科学院植物保护研究所 | Bait for raising orius sauteri and method for group raising |
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| CN105248384A (en) * | 2015-11-09 | 2016-01-20 | 云南省烟草公司玉溪市公司 | Method of improving Lipaphis erysimi breeding efficiency by using salicylic acid |
| CN105309393A (en) * | 2015-12-01 | 2016-02-10 | 贵州省植物保护研究所 | Novel ecological system breeding method for orius minutus |
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