CN102293182A - Orius sauteri raising method - Google Patents
Orius sauteri raising method Download PDFInfo
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- CN102293182A CN102293182A CN201110200775XA CN201110200775A CN102293182A CN 102293182 A CN102293182 A CN 102293182A CN 201110200775X A CN201110200775X A CN 201110200775XA CN 201110200775 A CN201110200775 A CN 201110200775A CN 102293182 A CN102293182 A CN 102293182A
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Abstract
The invention relates to the field of plant protection and the insect raising technology, in particular to an orius sauteri raising method adopting drosophila carcinophila as feedstuff. The method changes the traditional method of adopting aphid or frankliniella occidentalis as feedstuff, the defect of the orius sauteri raising caused by the problems of the two kinds of feedstuff per se is changed, the characteristics of short growth period of the drosophila carcinophila and low production environment requirement are utilized, the feedstuff basis is provided for the large-scale raising of the orius sauteri, and in addition, the feeding effect is very excellent.
Description
Technical field
The invention belongs to plant protection field and cultured insect technology, relate to the raising method of a kind of East Asia minute pirate bugs.
Background technology
The East Asia minute pirate bugs belongs to Semiptera section, is a kind of fore wing tool key, and tool is simple eye, the small-sized Lu Sheng stinkbug class insect of digitus 3 joints, and its adult and nymph can prey on software small worms such as aphid, thrips, small-sized lepidopterous larvae, and mite class and various worm's ovum.Because the East Asia minute pirate bugs is widely distributed, characteristics such as adaptability is strong, quantity is many, predatism is strong, be considered to a class predator insect than the good utilisation prospect is arranged, therefore, in recent years, for realizing the extensive raising of East Asia minute pirate bugs, its biology, oviposition substrate, feed and raising instrument etc. a series of researchs have been done both at home and abroad, though obtained certain achievement, raised big in addition gap apart from scale.
The breeding of East Asia minute pirate bugs has two conditions essential, and one is feed, and live body feed preferably can be edible for them as the mini pest of aphid, this class of Tetranychus cinnabarinus; Another condition is oviposition substrate exactly: the East Asia minute pirate bugs is liked laying eggs on the tender shoots of bean seedlings, so select oviposition substrate to select the kidney bean seedling that just grows tender shoots for use.Wherein be used to feed the feed of East Asia minute pirate bugs, general now employing aphid, Tetranychus cinnabarinus or Frankliniella occidentalis are as main feed, but in the above-mentioned feed, the cultivation overlong time of Frankliniella occidentalis, the Frankliniella occidentalis that the whole time approximately need could obtain as East Asia minute pirate bugs feed in about one month, and the condition of cultivating is higher, the rising that can bring cost; And when adopting aphid and Tetranychus cinnabarinus as feed, the cultivation time is also longer, and is general all more than 20 days, and the required condition of the cultivation of aphid is harsh more, and sanitary condition is poor, and the as easy as rolling off a log pollution that causes feeding environment causes the death of aphid; And when adopting Tetranychus cinnabarinus, have above-mentioned problem too, and when adopting above-mentioned two kinds of feeds, the lethality of East Asia minute pirate bugs is higher, can't satisfy the requirement of mass rearing.
Summary of the invention
Problems at the minute pirate bugs artificial feeding existence of existing East Asia, for extensive raising that can East Asia, better development East Asia minute pirate bugs, the inventor is through long-term experiment, a kind of method that adopts drosophila larvae to raise the East Asia minute pirate bugs as feed is provided, before changing, this method adopts aphid or Frankliniella occidentalis method as feed, changed because this two kinds of feed built-in problems and the East Asia minute pirate bugs raised the drawback of bringing, utilize the drosophila larvae growth cycle short, production environment requires low characteristics, for the extensive raising of East Asia minute pirate bugs provides the basis on the feed, and the effect of feeding is splendid.
The raising method of East Asia of the present invention minute pirate bugs mainly comprises the steps:
(1) cultivation of oviposition substrate is packed the nutrition soil for preparing in the pot for growing seedlings into, after watering, the embryo of kidney bean seed is imbedded downwards in the nutrition soil, 1/4 is exposed at outside the soil, watered one time water in per 24 hours, take out when treating kidney bean seedling plant height 5cm, root is preserved moisture with the absorbent cotton that soaks and is put into box for feeding pests and lay eggs for the East Asia minute pirate bugs.
(2) screening of worm kind will gather the East Asia minute pirate bugs adult that comes in the insectary with bean aphid raise set up stablize population after, filter out 1000 pairs of East Asia minute pirate bugs, the female male imago 20-25 that each box for feeding pests is inserted firm emergence is right, allow and lay eggs after its natural mating, collect East Asia minute pirate bugs ovum and use the worm kind as producing, changed 1 bean aphid feed in per 24 hours and changed 1 oviposition substrate in per 12 hours, collect 5 days ovum continuously, the band ovum matrix of collection places 3-5 ℃ of low temperature under preservation standby;
(3) raising of the East Asia minute pirate bugs oviposition substrate that will have an East Asia minute pirate bugs ovum places the box of laying eggs, under 25 ± 1 ℃, RH=75 ± 5%, 16L:8D condition, cultivate, can hatch in ovum 5-6 days, transfer to nymph in the plastics box for feeding pests of side opening with dactylethrae every day.The corrugated paper fringe is placed in the box for feeding pests bottom, and fresh clean kidney bean seedling, drosophila larvae and feed box thereof are placed on (minute pirate bugs day predation amount in single head adult East Asia is about: 30 of 10 of 60 of Frankliniella occidentalis or drosophila larvaes or bean aphids) on the corrugation paper slip.In feeding process, the drosophila larvae feed that more renewed in 2-3 days, it is stand-by to enter the adult stage after East Asia minute pirate bugs experience 4-5 the length of time (10-12 days).
Nutrition soil described in the step (1) is to be that 1: 1: 1 ratio mixes with black earth, vermiculite and peat composed of rotten mosses ash according to volume ratio.Because the East Asia minute pirate bugs is liked laying eggs on the tender shoots of bean seedlings, so oviposition substrate is selected the kidney bean seedling that just grows tender shoots for use, in whole feeding process, the kidney bean seedling not only can be used as oviposition substrate, also can be used as the water source of raising the East Asia minute pirate bugs.
The East Asia minute pirate bugs of original adoption picks up from the field in the step (2).
The box of laying eggs in the step (3) generally adopts the specification of 20cm*13cm*7cm, and the specification of box for feeding pests is 60cm*50cm*30cm, and the corrugated paper fringe in the box for feeding pests can separate nymph effectively, reduces internecine chance.In order to keep the vigor of drosophila larvae, need to place the drosophila larvae feed in the box for feeding pests, simultaneously creep into the problem that East Asia minute pirate bugs in the feed be can't see drosophila larvae, only spread very thin one deck feed in the drosophila larvae feed box, changed once in general 2-3 days for fear of drosophila larvae.
When adopting drosophila larvae to raise the East Asia minute pirate bugs, adopt the East Asia minute pirate bugs of the drosophila larvae raising 1-2 age in days of 1 age in days; Adopt the East Asia minute pirate bugs of the drosophila larvae raising 3-5 age in days of 2-3 age in days.Why design so mainly is to consider that the drosophila larvae growth rate is very fast, so that growth rate and predation that can balance East Asia minute pirate bugs, the size of drosophila larvae when just coming the regulation raising according to the age in days of East Asia minute pirate bugs.In order to play better effect, general drosophila larvae carries out independent cultivation, and its cultural method is as follows:
(1) configuration of medium: by weight, main component comprises: 600 parts in water, sugared 20-25 part, agar 8-10 part, corn flour or oatmeal 40-50 part, preservative 0.5-1 part, dusty yeast 10-15 part;
Its preparation method is: level is divided into two parts, a agar and the sugar of adding, another part adds corn flour or oatmeal, heating and constantly stirring respectively, treat that agar and sugar dissolve in water and is solution than thickness, corn flour or oatmeal dissolve in water and evenly distribute in the pasty state, again with the two admixed together stirring, slowly are heated to and boil, and keep boiling state 5 minutes, the medium that is homogeneous stops heating, slowly cooling;
When treating that the medium temperature is reduced to 74-76 ℃, add the preservative of formula ratio, stir, and divide the Cans that install to sterilization while hot;
Medium cools off but before not solidifying, spreads one deck dusty yeast on medium, to absorb the moisture on top layer, seals bottleneck with the bottle cap that has thin copper mesh, and the medium cooled and solidified can be stand-by.
Wherein, sugar can be selected soft white sugar or brown sugar for use, and preservative is a kind of in sorbic acid, propionic acid or the benzoic acid.
(2) raising of fruit bat: open standby medium bottleneck, clean a bottle inwall with 70% cotton ball soaked in alcohol, dry in the bottle of back and insert 10-15 kind of a fly, build bottle cap, being placed on 25 ± 1 ℃, RH=75 ± 5%, 16L:8D illumination condition cultivated 24 hours down, female fly post-coitum produces ovum in medium, will plant the fly taking-up after laying eggs and put into other blake bottle, and the medium of band ovum is hatched in the insectary and can be obtained larva in about 24 hours;
Larva hatches the back to be raised 2-3 days, adding distil water to 2/3 place in blake bottle, and larva climbs out of to enter in the water body and with screen filtration from medium isolates larva;
With the tweezers that go sharp passivation with the cotton ball soaked in alcohol sterilization after, drosophila larvae is taken out from feed, put into 0-4 ℃ of refrigerating box refrigeration 15-20 minute, put into the box for feeding pests of raising the East Asia minute pirate bugs after reducing the mobility of larva.
When treating that fruit bat grows into adult, put into fresh culture in the foster worm bottle, lay eggs, will be with the medium of ovum to collect, place 4-8 ℃ of refrigerator to protect and plant for it; The pupa of fruit bat also can be used for protecting kind, and the drosophila larvae experience just enters pupa time after three length of times, with pupa take out place 4-8 ℃ stand-by, fruit bat pupa time is four days under 25 ± 1 ℃ condition, can obtain adult rapidly.
The present invention adopts drosophila larvae to feed the East Asia minute pirate bugs as feed, has following beneficial effect:
1, because drosophila larvae just can be ripe about 1 week, than existing East Asia minute pirate bugs animal feed (bean aphid, Frankliniella occidentalis), the production cycle shortens significantly;
2, drosophila larvae contains nutrient components such as rich in protein, fatty acid, amino acid, chitin, vitamin, mineral element, macroelement and micronutrient levels are abundant, detected more than 17 kinds, fat content is about 12% in its dry powder, unsaturated fatty acid accounts for 68%, and essential fatty acid accounts for 36%, and nutrient component is comprehensive, contain East Asia minute pirate bugs needed each seed amino acid that grows, can satisfy the nutritional need that the East Asia minute pirate bugs grows and breeds preferably;
3, fruit bat secretes antibacterial substances such as a large amount of antibacterial peptides, can effectively prevent feed mold;
4, the production environment of fruit bat require low, production cost is low, breeding technology is ripe, growing is not subjected to seasonal effect, non-vulnerable to pollution, can be for a long time provides feed for the raising of East Asia minute pirate bugs.
In sum, before changing, technical scheme of the present invention adopts aphid or Frankliniella occidentalis method as feed, changed because this two kinds of feed built-in problems and the East Asia minute pirate bugs raised the drawback of bringing, utilize the drosophila larvae growth cycle short, production environment requires low characteristics, for the extensive raising of East Asia minute pirate bugs provides the basis on the feed, and the effect of feeding is splendid.
Embodiment
Embodiment 1
Raise East Asia minute pirate bugs adult with drosophila larvae, the cultural method of drosophila larvae is:
(1) configuration of medium: by weight, main component comprises: 600 parts in water, 22 parts in brown sugar, 10 parts in agar, 45 parts of oatmeals, 1 part of sorbic acid, 15 parts of dusty yeasts;
Its preparation method is: level is divided into two parts, a agar and the brown sugar of adding, another part adds oatmeal, heating and constantly stirring respectively, treat that agar and brown sugar dissolve in water and is solution than thickness, oatmeal dissolves in water and evenly distributes in the pasty state, again with the two admixed together stirring, slowly is heated to and boils, and keep boiling state 5 minutes, the medium that is homogeneous stops heating, slowly cooling;
When treating that the medium temperature is reduced to 74 ℃, add the sorbic acid of formula ratio, stir, and divide the Cans that install to sterilization while hot;
Medium cools off but before not solidifying, spreads one deck dusty yeast on medium, to absorb the moisture on top layer, seals bottleneck with the bottle cap that has thin copper mesh, and the medium cooled and solidified can be stand-by;
(2) raising of fruit bat: open standby medium bottleneck, clean a bottle inwall with 70% cotton ball soaked in alcohol, dry in the bottle of back and insert 12 pairs of kind flies, build bottle cap, being placed on 25 ± 1 ℃, RH=75 ± 5%, 16L:8D illumination condition cultivated 24 hours down, female fly post-coitum produces ovum in medium, will plant the fly taking-up after laying eggs and put into other blake bottle, and the medium of band ovum is hatched in the insectary and can be obtained larva in about 24 hours;
Larva hatches the back to be raised 2 days, adding distil water to 2/3 place in blake bottle, and larva climbs out of to enter in the water body and with screen filtration from medium isolates larva;
With the tweezers that go sharp passivation with the cotton ball soaked in alcohol sterilization after, drosophila larvae is taken out from feed, put into 2 ℃ of refrigerating boxes refrigerations 17 minutes, put into the box for feeding pests of raising the East Asia minute pirate bugs after reducing the mobility of larva.
The drosophila larvae of above-mentioned cultivation is used for the raising of East Asia minute pirate bugs, mainly comprises the steps:
(1) cultivation of oviposition substrate is with 1: 1: 1 by volume ratio mixing of black earth, vermiculite and turfy soil, pack in the pot for growing seedlings, after watering, the embryo of kidney bean seed is imbedded downwards in the nutrition soil, 1/4 is exposed at outside the soil, watered once in per 24 hours, and took out when treating kidney bean seedling plant height 5cm, root is preserved moisture with the absorbent cotton that soaks and is put into box for feeding pests and lay eggs for the East Asia minute pirate bugs;
(2) screening of worm kind will gather the East Asia minute pirate bugs adult that comes in the insectary with bean aphid raise set up stablize population after, filter out 1000 pairs of East Asia minute pirate bugs, each box for feeding pests is inserted 20 pairs of the female male imagos of firm emergence, allow and lay eggs after its natural mating, collect East Asia minute pirate bugs ovum and use the worm kind as producing, changed 1 bean aphid feed in per 24 hours and changed 1 oviposition substrate in per 12 hours, collect 5 days ovum continuously, the band ovum matrix of collection places 4 ℃ of low temperature under preservation standby;
(3) raising of the East Asia minute pirate bugs oviposition substrate that will have an East Asia minute pirate bugs ovum places the box of laying eggs, under 25 ± 1 ℃, RH=75 ± 5%, 16L:8D condition, cultivate, ovum can be hatched in 5 days, transfer to nymph in the plastics box for feeding pests of side opening every day with dactylethrae, the corrugated paper fringe is placed in the box for feeding pests bottom, and fresh clean kidney bean seedling, drosophila larvae and feed box thereof are placed on (minute pirate bugs day predation amount in single head adult East Asia is about: 30 of 10 of 60 of Frankliniella occidentalis or drosophila larvaes or bean aphids) on the corrugation paper slip.The drosophila larvae feed that more renewed in per 2 days, it is stand-by to enter the adult stage after the East Asia minute pirate bugs experiences 4 length of times.
Embodiment 2
Raise East Asia minute pirate bugs adult with drosophila larvae, the cultural method of drosophila larvae is:
(1) configuration of medium: by weight, main component comprises: 600 parts in water, 20 parts of soft white sugars, 8 parts in agar, 40 parts of corn flour, 0.7 part in benzoic acid, 10 parts of dusty yeasts;
Its preparation method is: level is divided into two parts, a agar and the soft white sugar of adding, another part adds corn flour, heating and constantly stirring respectively, treat that agar and soft white sugar dissolve in water and be solution than thickness, corn flour dissolves in water and evenly distributes in the pasty state, again with the two admixed together stirring, slowly is heated to and boils, and keep boiling state 5 minutes, the medium that is homogeneous stops heating, slowly cooling;
When treating that the medium temperature is reduced to 76 ℃, add the benzoic acid of formula ratio, stir, and divide the Cans that install to sterilization while hot;
Medium cools off but before not solidifying, spreads one deck dusty yeast on medium, to absorb the moisture on top layer, seals bottleneck with the bottle cap that has thin copper mesh, and the medium cooled and solidified can be stand-by;
(2) raising of fruit bat: open standby medium bottleneck, clean a bottle inwall with 70% cotton ball soaked in alcohol, dry in the bottle of back and insert 15 pairs of kind flies, build bottle cap, being placed on 25 ± 1 ℃, RH=75 ± 5%, 16L:8D illumination condition cultivated 24 hours down, female fly post-coitum produces ovum in medium, will plant the fly taking-up after laying eggs and put into other blake bottle, and the medium of band ovum is hatched in the insectary and can be obtained larva in about 24 hours;
Larva hatches the back to be raised 3 days, adding distil water to 2/3 place in blake bottle, and larva climbs out of to enter in the water body and with screen filtration from medium isolates larva;
With the tweezers that go sharp passivation with the cotton ball soaked in alcohol sterilization after, drosophila larvae is taken out from feed, put into 4 ℃ of refrigerating boxes refrigerations 20 minutes, put into the box for feeding pests of raising the East Asia minute pirate bugs after reducing the mobility of larva.
The drosophila larvae of above-mentioned cultivation is used for the raising of East Asia minute pirate bugs, mainly comprises the steps:
(1) cultivation of oviposition substrate with black earth, vermiculite and turfy soil in 1: 1: 1 ratio mixing, pack in the pot for growing seedlings, after watering, the embryo of kidney bean seed is imbedded downwards in the nutrition soil, 1/4 is exposed at outside the soil, watered water one time in per 24 hours, and took out when treating kidney bean seedling plant height 5cm, root is preserved moisture with the absorbent cotton that soaks and is put into box for feeding pests and lay eggs for the East Asia minute pirate bugs;
(2) screening of worm kind will gather the East Asia minute pirate bugs adult that comes in the insectary with bean aphid raise set up stablize population after, filter out 1000 pairs of East Asia minute pirate bugs, each box for feeding pests is inserted 25 pairs of the female male imagos of firm emergence, allow and lay eggs after its natural mating, collect East Asia minute pirate bugs ovum and use the worm kind as producing, changed 1 bean aphid feed in per 24 hours and changed 1 oviposition substrate in per 12 hours, collect 5 days ovum continuously, the band ovum matrix of collection places 3 ℃ of low temperature under preservation standby;
(3) raising of the East Asia minute pirate bugs oviposition substrate that will have an East Asia minute pirate bugs ovum places the box of laying eggs, at 25 ± 1 ℃, RH=75 ± 5%, cultivate under the 16L:8D condition, ovum can be hatched in 6 days, transfer to nymph in the plastics box for feeding pests of side opening every day with dactylethrae, the corrugated paper fringe is placed in the box for feeding pests bottom, with fresh clean kidney bean seedling, drosophila larvae and feed box be placed on (minute pirate bugs day predation amount in single head adult East Asia is about: 30 of 10 of 60 of Frankliniella occidentalis or drosophila larvaes or bean aphids) on the corrugation paper slip, the drosophila larvae feed that more renewed in per 3 days, it is stand-by to enter the adult stage after the East Asia minute pirate bugs experiences 5 length of times.
Embodiment 3
Raise East Asia minute pirate bugs adult with drosophila larvae, the cultural method of drosophila larvae is:
(1) configuration of medium: by weight, main component comprises: 600 parts in water, 25 parts in brown sugar, 9 parts in agar, 50 parts of oatmeals, 0.5 part of propionic acid, 12 parts of dusty yeasts;
Its preparation method is: level is divided into two parts, a agar and the brown sugar of adding, another part adds oatmeal, heating and constantly stirring respectively, treat that agar and brown sugar dissolve in water and is solution than thickness, oatmeal dissolves in water and evenly distributes in the pasty state, again with the two admixed together stirring, slowly is heated to and boils, and keep boiling state 5 minutes, the medium that is homogeneous stops heating, slowly cooling;
When treating that the medium temperature is reduced to 75 ℃, add the propionic acid of formula ratio, stir, and divide the Cans that install to sterilization while hot;
Medium cools off but before not solidifying, spreads one deck dusty yeast on medium, to absorb the moisture on top layer, seals bottleneck with the bottle cap that has thin copper mesh, and the medium cooled and solidified can be stand-by;
(2) raising of fruit bat: open standby medium bottleneck, clean a bottle inwall with 70% cotton ball soaked in alcohol, dry in the bottle of back and insert 10 pairs of kind flies, build bottle cap, being placed on 25 ± 1 ℃, RH=75 ± 5%, 16L:8D illumination condition cultivated 24 hours down, female fly post-coitum produces ovum in medium, will plant the fly taking-up after laying eggs and put into other blake bottle, and the medium of band ovum is hatched in the insectary and can be obtained larva in about 24 hours;
Larva hatches the back to be raised 2 days, adding distil water to 2/3 place in blake bottle, and larva climbs out of to enter in the water body and with screen filtration from medium isolates larva;
With the tweezers that go sharp passivation with the cotton ball soaked in alcohol sterilization after, drosophila larvae is taken out from feed, put into 0 ℃ of refrigerating box refrigeration 15 minutes, put into the box for feeding pests of raising the East Asia minute pirate bugs after reducing the mobility of larva.
The drosophila larvae of above-mentioned cultivation is used for the raising of East Asia minute pirate bugs, mainly comprises the steps:
(1) cultivation of oviposition substrate with black earth, vermiculite and turfy soil in 1: 1: 1 ratio mixing, pack in the pot for growing seedlings, after watering, the embryo of kidney bean seed is imbedded downwards in the nutrition soil, 1/4 is exposed at outside the soil, watered water one time in per 24 hours, and took out when treating kidney bean seedling plant height 5cm, root is preserved moisture with the absorbent cotton that soaks and is put into box for feeding pests and lay eggs for the East Asia minute pirate bugs;
(2) screening of worm kind will gather the East Asia minute pirate bugs adult that comes in the insectary with bean aphid raise set up stablize population after, filter out 1000 pairs of East Asia minute pirate bugs, each box for feeding pests is inserted 22 pairs of the female male imagos of firm emergence, allow and lay eggs after its natural mating, collect East Asia minute pirate bugs ovum and use the worm kind as producing, changed 1 bean aphid feed in per 24 hours and changed 1 oviposition substrate in per 12 hours, collect 5 days ovum continuously, the band ovum matrix of collection places 5 ℃ of low temperature under preservation standby;
(3) raising of the East Asia minute pirate bugs oviposition substrate that will have an East Asia minute pirate bugs ovum places the box of laying eggs, under 25 ± 1 ℃, RH=75 ± 5%, 16L:8D condition, cultivate, ovum can be hatched in 5 days, transfer to nymph in the plastics box for feeding pests of side opening every day with dactylethrae, the corrugated paper fringe is placed in box for feeding pests bottom, with fresh clean kidney bean seedling, drosophila larvae and feed box be placed on (minute pirate bugs day predation amount in single head adult East Asia is about: 30 of 10 of 60 of Frankliniella occidentalis or drosophila larvaes or bean aphids) on the corrugation paper slip.The drosophila larvae feed that more renewed in per 2 days, it is stand-by to enter the adult stage after the East Asia minute pirate bugs experiences 5 length of times.
Comparative example 1
Raise East Asia minute pirate bugs adult with bean aphid, bean aphid adopts prior art to cultivate, and approximately can supply in 4-5 days to raise general 8-10 days generation.Raising step according to the East Asia minute pirate bugs described in the embodiment 1 is fed.
Comparative example 2
With Frankliniella occidentalis raising East Asia minute pirate bugs adult, Frankliniella occidentalis adopts prior art to cultivate, and approximately can generally breed a generation in 25-30 days for raising in 5-7 days.Raising step according to the East Asia minute pirate bugs described in the embodiment 1 is fed.
Embodiment 1 is compared with comparative example 1 and 2, finds fruit bat and Frankliniella occidentalis breeding grub feeding effect the best after two generations, bean aphid during as feed effect relatively poor.The East Asia minute pirate bugs nymph lethality that drosophila larvae is raised is low, 10%-15% only, and it is long for about 12.21d that nymphal development is gone through the phase, can be the enough nutriments of adult stage accumulation, prolonged life-span and the egg-laying period of adult.The adult mean lifetime is about 19d, the longlyest reaches 26 days.The first generation adult egg-laying period, egg production peak time was at the 2nd to 6 day about 14 days, and single female average egg laying amount is 98.The second generation egg-laying period is elongated, and about 17 days, the egg laying amount of every day is average, and between 5 to 9, but single female egg laying amount does not reduce.
The East Asia minute pirate bugs nymph lethality that Frankliniella occidentalis is raised is 10%-15%, and it is 12.19d that nymphal development is gone through the phase.The adult mean lifetime is about 19d, the longlyest reaches 24 days, and egg production peak time concentrates on the 2nd to 6 day, and single female average egg laying amount is 101, about average egg-laying period 14d.
The East Asia minute pirate bugs nymphal development that bean aphid is raised is gone through short only 11.72d of phase, lethality about 20%, and single female egg laying amount is about 66.About average egg-laying period 11d, mean lifetime is about 14d.
Frankliniella occidentalis is that present feeding effect is best, but operating difficulties, cost is than higher, and the feeding effect of drosophila larvae of the present invention and Frankliniella occidentalis is suitable, and than the technology that Frankliniella occidentalis is fed, reduced the environmental pollution and the production cost that cause when the live body feed is raised, simple to operate, production cycle shortens significantly, is enough to a large amount of promotion and application.
Claims (7)
1. the raising method of an East Asia minute pirate bugs is characterized in that: adopt drosophila larvae to raise the East Asia minute pirate bugs.
2. the raising method of East Asia according to claim 1 minute pirate bugs is characterized in that: mainly comprise the steps:
(1) cultivation of oviposition substrate is packed the nutrition soil for preparing in the pot for growing seedlings into, after watering, the embryo of kidney bean seed is imbedded downwards in the nutrition soil, 1/4 is exposed at outside the soil, watered one time water in per 24 hours, take out when treating kidney bean seedling plant height 5cm, root is preserved moisture with the absorbent cotton that soaks and is put into box for feeding pests and lay eggs for the East Asia minute pirate bugs;
(2) screening of worm kind will gather the East Asia minute pirate bugs adult that comes in the insectary with bean aphid raise set up stablize population after, filter out 1000 pairs of East Asia minute pirate bugs, the female male imago 20-25 that each box for feeding pests is inserted firm emergence is right, allow and lay eggs after its natural mating, collect East Asia minute pirate bugs ovum and use the worm kind as producing, changed 1 bean aphid feed in per 24 hours and changed 1 oviposition substrate in per 12 hours, collect 5 days ovum continuously, the band ovum matrix of collection places 3-5 ℃ of low temperature under preservation standby;
(3) raising of the East Asia minute pirate bugs oviposition substrate that will have an East Asia minute pirate bugs ovum places the box of laying eggs, under 25 ± 1 ℃, RH=75 ± 5%, 16L:8D condition, cultivate, can hatch in ovum 5-6 days, transfer to nymph in the plastics box for feeding pests of side opening every day with dactylethrae, the corrugated paper fringe is placed in the box for feeding pests bottom, fresh clean kidney bean seedling, drosophila larvae and feed box thereof are placed on the corrugation paper slip, the drosophila larvae feed that more renewed in 2-3 days, it is stand-by to enter the adult stage after the East Asia minute pirate bugs experiences 4-5 the length of time.
3. the raising method of East Asia according to claim 1 and 2 minute pirate bugs is characterized in that: the East Asia minute pirate bugs that adopts the drosophila larvae raising 1-2 age in days of 1 age in days; Adopt the East Asia minute pirate bugs of the drosophila larvae raising 3-5 age in days of 2-3 age in days.
4. the raising method of East Asia according to claim 3 minute pirate bugs is characterized in that: described drosophila larvae cultural method mainly comprises the steps:
(1) configuration of medium: by weight, main component comprises: 600 parts in water, sugared 20-25 part, agar 8-10 part, corn flour or oatmeal 40-50 part, preservative 0.5-1 part, dusty yeast 10-15 part;
Its preparation method is: level is divided into two parts, a agar and the sugar of adding, another part adds corn flour or oatmeal, heating and constantly stirring respectively, treat that agar and sugar dissolve in water and is solution than thickness, corn flour or oatmeal dissolve in water and evenly distribute in the pasty state, again with the two admixed together stirring, slowly are heated to and boil, and keep boiling state 5 minutes, the medium that is homogeneous stops heating, slowly cooling;
When treating that the medium temperature is reduced to 74-76 ℃, add the preservative of formula ratio, stir, and divide the Cans that install to sterilization while hot;
Medium cools off but before not solidifying, spreads one deck dusty yeast on medium, to absorb the moisture on top layer, seals bottleneck with the bottle cap that has thin copper mesh, and the medium cooled and solidified can be stand-by;
(2) raising of fruit bat: open standby medium bottleneck, clean a bottle inwall with 70% cotton ball soaked in alcohol, dry in the bottle of back and insert 10-15 kind of a fly, build bottle cap, being placed on 25 ± 1 ℃, RH=75 ± 5%, 16L:8D illumination condition cultivated 24 hours down, female fly post-coitum produces ovum in medium, will plant the fly taking-up after laying eggs and put into other blake bottle, and the medium of band ovum is hatched in the insectary and can be obtained larva in about 24 hours;
Larva hatches the back to be raised 2-3 days, adding distil water to 2/3 place in blake bottle, and larva climbs out of to enter in the water body and with screen filtration from medium isolates larva;
With the tweezers that go sharp passivation with the cotton ball soaked in alcohol sterilization after, drosophila larvae is taken out from feed, put into 0-4 ℃ of refrigerating box refrigeration 15-20 minute, put into the box for feeding pests of raising the East Asia minute pirate bugs after reducing the mobility of larva.
5. the raising method of East Asia according to claim 4 minute pirate bugs is characterized in that: described preservative is a kind of in sorbic acid, propionic acid or the benzoic acid.
6. the raising method of East Asia according to claim 4 minute pirate bugs is characterized in that: described sugar is a kind of in soft white sugar or the brown sugar.
7. the raising method of East Asia according to claim 2 minute pirate bugs is characterized in that: the nutrition soil described in the step (1) is to mix at 1: 1: 1 black earth, vermiculite and turfy soil according to volume ratio.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110200775 CN102293182B (en) | 2011-07-18 | 2011-07-18 | Orius sauteri raising method |
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| CN103444632A (en) * | 2012-06-01 | 2013-12-18 | 株式会社易可盈 | Raising method utilizing predators of Galleria mellonella eggs |
| CN103704176A (en) * | 2013-11-29 | 2014-04-09 | 贵州大学 | Orius strigicollis raising method |
| CN103843728A (en) * | 2014-03-14 | 2014-06-11 | 汪岩屹 | Method and device for propagating fruit flies by utilizing liquid culture medium |
| CN103931565A (en) * | 2014-04-22 | 2014-07-23 | 山东省农业科学院植物保护研究所 | Artificial breeding method of Suzuki fruit flies for testing |
| CN104222006A (en) * | 2013-06-21 | 2014-12-24 | 王伯明 | Method for artificial mass production of orius |
| CN104799100A (en) * | 2015-04-29 | 2015-07-29 | 华中农业大学 | Semisolid artificial feed for natural enemy insect orius strigicollis and preparation method of semisolid artificial feed |
| CN105123351A (en) * | 2015-07-20 | 2015-12-09 | 胡永军 | Method for controlling protected vegetable thrips by utilizing Oriussauteri |
| CN105248382A (en) * | 2015-11-01 | 2016-01-20 | 贵州大学 | Breeding method and device of orius similis |
| CN105325377A (en) * | 2015-12-01 | 2016-02-17 | 贵州省植物保护研究所 | Large-scale propagation method for orius minutus |
| CN105660546A (en) * | 2016-04-01 | 2016-06-15 | 云南省农业科学院花卉研究所 | Method for feeding frankliniella occidentalis indoors |
| CN106172253A (en) * | 2016-08-01 | 2016-12-07 | 山东省农业科学院植物保护研究所 | A kind of Orius batch production collection method and collection device |
| CN106305628A (en) * | 2016-08-22 | 2017-01-11 | 北京阔野田园生物技术有限公司 | Method for scale propagation of amphiareus obscuriceps |
| CN107279475A (en) * | 2017-08-10 | 2017-10-24 | 中国农业科学院植物保护研究所 | A kind of method using the prey artificial feeding predatism stinkbug kept in cold storage |
| CN109006712A (en) * | 2018-09-12 | 2018-12-18 | 安徽师范大学 | A kind of method for breeding and breeding apparatus of Ling Mushi drosophila |
| CN109566541A (en) * | 2018-12-17 | 2019-04-05 | 浙江省农业科学院 | A kind of preparation method and application of Similis oviposition medium |
| CN109644950A (en) * | 2019-02-11 | 2019-04-19 | 广东省烟草南雄科学研究所 | A kind of Harpactor fuscipes large-scale breeding method |
| CN111493031A (en) * | 2020-05-18 | 2020-08-07 | 河南省农业科学院烟草研究所 | Method for reducing mortality rate of middle-aged and low-aged nymphs in raising orius sauteri |
| CN112997972A (en) * | 2021-03-30 | 2021-06-22 | 河南省农业科学院烟草研究所 | Ornitus avidus egg preservation method suitable for large-scale breeding |
| CN113826587A (en) * | 2021-10-21 | 2021-12-24 | 广西壮族自治区农业科学院 | Indoor breeding method for adelphocoris suturalis |
| CN114794027A (en) * | 2022-05-15 | 2022-07-29 | 中国医学科学院药用植物研究所云南分所 | Indoor propagation method for cyrtorhinus lividipennis |
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| CN103444632A (en) * | 2012-06-01 | 2013-12-18 | 株式会社易可盈 | Raising method utilizing predators of Galleria mellonella eggs |
| CN103444632B (en) * | 2012-06-01 | 2015-12-09 | 株式会社易可盈 | Utilize the raising method of the predator of large wax moth ovum |
| CN104222006B8 (en) * | 2013-06-21 | 2016-12-21 | 中国农业科学院植物保护研究所 | A kind of artificial a large amount of methods producing minute pirate bugs |
| CN104222006B (en) * | 2013-06-21 | 2016-08-10 | 首伯农(北京)生物技术有限公司 | A kind of artificial a large amount of methods producing minute pirate bugs |
| CN104222006A (en) * | 2013-06-21 | 2014-12-24 | 王伯明 | Method for artificial mass production of orius |
| CN103704176A (en) * | 2013-11-29 | 2014-04-09 | 贵州大学 | Orius strigicollis raising method |
| CN103704176B (en) * | 2013-11-29 | 2015-05-06 | 贵州大学 | Orius strigicollis raising method |
| CN103843728B (en) * | 2014-03-14 | 2015-08-19 | 汪岩屹 | A kind of method and device utilizing liquid nutrient medium breeding fruit bat |
| CN103843728A (en) * | 2014-03-14 | 2014-06-11 | 汪岩屹 | Method and device for propagating fruit flies by utilizing liquid culture medium |
| CN103931565A (en) * | 2014-04-22 | 2014-07-23 | 山东省农业科学院植物保护研究所 | Artificial breeding method of Suzuki fruit flies for testing |
| CN103931565B (en) * | 2014-04-22 | 2015-12-02 | 山东省农业科学院植物保护研究所 | A kind of rearing method for examination Ling Mushi fruit bat |
| CN104799100A (en) * | 2015-04-29 | 2015-07-29 | 华中农业大学 | Semisolid artificial feed for natural enemy insect orius strigicollis and preparation method of semisolid artificial feed |
| CN104799100B (en) * | 2015-04-29 | 2018-05-04 | 华中农业大学 | A kind of natural enemy insect Similis semisolid man-made feeds and preparation method thereof |
| CN105123351B (en) * | 2015-07-20 | 2018-01-30 | 山东永盛农业发展有限公司 | A kind of method of Orius facility for prevention and control vegetables thrips |
| CN105123351A (en) * | 2015-07-20 | 2015-12-09 | 胡永军 | Method for controlling protected vegetable thrips by utilizing Oriussauteri |
| CN105248382A (en) * | 2015-11-01 | 2016-01-20 | 贵州大学 | Breeding method and device of orius similis |
| CN105325377A (en) * | 2015-12-01 | 2016-02-17 | 贵州省植物保护研究所 | Large-scale propagation method for orius minutus |
| CN105660546A (en) * | 2016-04-01 | 2016-06-15 | 云南省农业科学院花卉研究所 | Method for feeding frankliniella occidentalis indoors |
| CN105660546B (en) * | 2016-04-01 | 2018-02-06 | 云南省农业科学院花卉研究所 | A kind of Frankliniella occidentalis indoor feeding method |
| CN106172253B (en) * | 2016-08-01 | 2019-03-29 | 山东省农业科学院植物保护研究所 | A kind of Orius batch production collection method and collection device |
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| CN106305628A (en) * | 2016-08-22 | 2017-01-11 | 北京阔野田园生物技术有限公司 | Method for scale propagation of amphiareus obscuriceps |
| CN107279475A (en) * | 2017-08-10 | 2017-10-24 | 中国农业科学院植物保护研究所 | A kind of method using the prey artificial feeding predatism stinkbug kept in cold storage |
| CN109006712A (en) * | 2018-09-12 | 2018-12-18 | 安徽师范大学 | A kind of method for breeding and breeding apparatus of Ling Mushi drosophila |
| CN109566541A (en) * | 2018-12-17 | 2019-04-05 | 浙江省农业科学院 | A kind of preparation method and application of Similis oviposition medium |
| CN109644950A (en) * | 2019-02-11 | 2019-04-19 | 广东省烟草南雄科学研究所 | A kind of Harpactor fuscipes large-scale breeding method |
| CN109644950B (en) * | 2019-02-11 | 2021-11-26 | 广东省烟草南雄科学研究所 | Large-scale breeding method for red-color lygus lucorum |
| CN111493031A (en) * | 2020-05-18 | 2020-08-07 | 河南省农业科学院烟草研究所 | Method for reducing mortality rate of middle-aged and low-aged nymphs in raising orius sauteri |
| CN112997972A (en) * | 2021-03-30 | 2021-06-22 | 河南省农业科学院烟草研究所 | Ornitus avidus egg preservation method suitable for large-scale breeding |
| CN113826587A (en) * | 2021-10-21 | 2021-12-24 | 广西壮族自治区农业科学院 | Indoor breeding method for adelphocoris suturalis |
| CN114794027A (en) * | 2022-05-15 | 2022-07-29 | 中国医学科学院药用植物研究所云南分所 | Indoor propagation method for cyrtorhinus lividipennis |
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