CN103843728A - Method and device for propagating fruit flies by utilizing liquid culture medium - Google Patents
Method and device for propagating fruit flies by utilizing liquid culture medium Download PDFInfo
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- CN103843728A CN103843728A CN201410098356.3A CN201410098356A CN103843728A CN 103843728 A CN103843728 A CN 103843728A CN 201410098356 A CN201410098356 A CN 201410098356A CN 103843728 A CN103843728 A CN 103843728A
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Abstract
The invention relates to a method and a device for propagating fruit flies by utilizing a liquid culture medium. The method comprises the following steps that (1) the liquid culture medium is prepared; (2) a fruit fly cage mode is used for feeding adult fries; (3) larvae are fed, and flyblows are obtained: (3-1) a feeding tank for feeding fruit fly larvae is manufactured; (3-2) the liquid culture medium is added into the feeding tank; (3-3) the feeding tank is put into a fly cage; (3-4) fruit flyblows are obtained; (4) after the fruit fly larvae are fed to the aging state, old and mature larvae are eluted and filtered; (5) the larvae are placed in a bag, after the fruit fly larvae are pupated, fruit fly pupae are cut off together with the bag, and undamaged fruit fly pupae are obtained. The method and the device have the advantages that various insect states, particularly older larvae and fruit fly larvae, can be easily obtained, the surfaces of the older larvae and the pupae of the obtained fruit flies are clean, and the pupa body is complete and is not damaged.
Description
Technical field
The present invention relates to biological technical field, be specifically related to a kind of method and device that utilizes liquid nutrient medium breeding fruit bat.
Background technology
Fruit bat belongs to Drosophilidae Drosophilidae insect; The saprophytic property of such insect, is distributed widely in the climatic region such as global temperate zone and the torrid zone, is common insect in life; Drosophila melanogaster Drosophila melanogaster, Ling Mushi fruit bat D.suzukii and extra large De Shi fruit bat D.hydei etc. are wherein important pests on the fruit such as cherry and red bayberry.In orchard, the ripe or fruit that starts to decay of fruit bat harm, adult produces ovum under fruit surface or pericarp, ovum hatch after with larva damage; Along with the moth food of larva, fruit gradually softening, browning, rot and come off.The harm of fruit bat affects the output of relevant fruit; Fruit bat also has influence on the marketing fresh of relevant fruit simultaneously, and on fruit, the existence of drosophila larvae causes relevant fruit price obviously to drop.
The improvement of orchard fruit bat is main by methods such as chemistry, physical mechanical and cultural controls at present.Chemical prevention and control method mainly utilizes the biologically active Control pests of chemical agent; Physico mechanical control method utilizes the physical agent such as light, heat and application machine or other instrument to control insect, lures worm liquid, insect-sticking plate, property to lure core etc. as utilized; Cultural control method refers to the administrative skill of utilizing a series of tillage and cultivations, as links such as orchard addressing, management and fruit harvestings.Except above-mentioned method, biological control method is also the important method of Control pests outburst, and biological control method refers to the method for utilizing natural enemy to carry out Control pests.
In the time utilizing said method to study fruit bat, generally need amount reproduction fruit bat, obtain the fruit bat of specific worm state or specific developmental stage.Take biological control method as example, in the time utilizing parasitic wasp to carry out the biological control to fruit bat, fruit bat parasitic wasp natural enemy is carried out biological control and is one of key link to the parasitic effects evaluation of parasitic wasp.Some parasitic wasps are usually only directed to specific worm state, and as the parasitic wasp in pupa time that fly pupa tiny golden wasp Pachycrepoideus vindemmiae is fruit bat, the breeding to this parasitic wasp and the evaluation of parasitic effects all need a large amount of fruit bat pupas.In existing fruit bat propagation technique, the cultivation of fruit bat all depends on solid culture medium, while breeding as the red bayberry fruit bat of Chinese patent (publication number CN102578052A) proposition, add agar in feed, solid culture primary surface uses spray bottle to spray with fresh milk and orange juice; Chinese patent (publication number CN102342376) proposes a kind of wheat bran medium of use for laboratory, is also solid culture medium.In some documents, also all in medium, add agar and other component, raise fruit bat.When solid culture based method is raised fruit bat for scientific research on a small scale in laboratory, there is certain advantage, as fruit bat can long period conservation breeding in the fruit bat pipe that is added with solid culture medium.But mass rearing fruit bat and while obtaining specific worm state (as pupa), be but difficult to effectively obtain complete fruit bat pupa, reduced production efficiency.
Fruit bat pupa is puparium type, the about 3mm of length, and pupa surface is very thin puparium, is pupal cell in puparium.Fruit bat pupa is usually attached to culture vessel or media surface, in the time collecting fruit bat pupa, usually be difficult to obtain complete fruit bat pupa, and the integrality of fruit bat pupa has influence on the parasitic rate of parasitic wasp, the breakage rate of fruit bat pupa causes the production efficiency of parasitic wasp to decline, rigorous not to the evaluation of parasitic wasp parasitic effects.
Summary of the invention
Cultivating in order to overcome solid culture medium traditionally the shortcoming that obtains not easily separated each worm state fruit bat on drosophila larvae, the invention provides a kind of method and device that utilizes liquid nutrient medium breeding fruit bat.Concrete technical scheme is as follows:
A method of utilizing liquid nutrient medium breeding fruit bat, comprises the steps:
(1) obtaining liq medium;
(2) use fruit bat cage mode to raise into fly;
(3) breeding grub obtain fly blow:
(3-1) make the raising tank of raising drosophila larvae;
(3-2) liquid nutrient medium is added to raising tank;
(3-3) raising tank is put into fly cage;
(3-4) obtain fruit bat ovum;
(4) raise aging to drosophila larvae after, wash-out and filter collect mature larva;
(5) be placed in bag, after drosophila larvae is pupated, fruit bat pupa cut together with bag, obtain undamaged fruit bat pupa.
Further, step (1) comprising:
(1-1) obtaining liq medium: the component of medium and proportioning are: 5 parts of dusty yeasts: 55 parts of sucrose: 51 parts of corn flour: 500 parts, water;
(1-2) preservation of medium and for subsequent use.
Further, step (1-1), in the time that medium configures, is first boiled water heating, adds sucrose and the corn flour of respective amount, is cooled to normal temperature, then adds dusty yeast stirring and evenly mixing after fully mixing; And/or, in step (1-2), the medium configuring is put into 6 ℃ of conventional refrigerators for subsequent use.
Further, step (2) comprising:
(2-1) fly cage is supported in design;
(2-2) in fly cage, place fruit bat adult.
Further, in step (2-1), making the length of sides with 100 order nylon wires is the fly cage main body of cube recuperating of 50 centimetres, the cage hole of supporting fly cage main body side opening one diameter 15cm, by with 100 order nylon wires make sleeve and hole, cage hole along stitching, sleeve place tightens with rope; In step (2-2), in fly cage, place approximately 1000 of Drosophila melanogaster adults.
Further, in step (3):
(3-1) in, make the ventilative raising tank of raising drosophila larvae: in volume is the cylindrical transparent plastic can of 300mL, raise, open the circular hole that a diameter is about 6cm in the middle of the screw lid of tank mouth, envelope is with 100 order nylon wires, for raising the ventilative of tank;
(3-2) liquid nutrient medium is added to and raises tank and above medium, lay gauze: raise in tank and put into the about 50mL of above-mentioned medium, then add two of circular Gauzes in media surface, lay eggs and adhere to larva for fruit bat;
(3-3) raising tank is put into fly cage periodic replacement: raising tank is put into fly cage, within every 12 hours, regularly take out and cover tightly with above-mentioned ventilative screw lid, and changing the raising tank that contains fresh culture; Open screw lid every day, with a small amount of water of spray bottle sprinkling, to prevent gauze surface desiccation;
(3-4) obtain fruit bat ovum.
Further, in step (4):
Collect mature larva with water-washing method wash-out filtration; And/or,
Raise after 4 days, raise in tank and occurred that a small amount of fruit bat pupates, most of larva enters the aged stage; The feed of raising in tank is poured in basin together with gauze, put the warm water of approximately 25 ℃ in basin, concussion comes off drosophila larvae and takes out gauze, uses the mesh screen that 40 order nylon wires are made to filter, and removes medium residue.
Further, in step (5): be placed in food preserving bag, draw the larger water droplet on larva surface with blotting paper, sack place does not seal bundle; After 2 days, in bag, drosophila larvae is pupated, and uses scissors that fruit bat pupa is cut together with plastic sack, obtains undamaged fruit bat pupa.
Further, it,, for raising tank, comprises nylon wire, spiral cover and tank body, and wherein, tank body is cylindrical transparent plastic can, and spiral cover is arranged on the tank mouth of tank body, and nylon wire is arranged on the circular hole of spiral cover central authorities.
Further, described cylindrical transparent plastic can diameter is about 8cm, height is about 6cm, and described circular hole is the circular hole that the central diameter that uses hot knife to cut of screw lid is about 6cm, and it adopts thermosol gelgun to seal with nylon wire described in 100 orders; And/or, also comprise 2 layers of circular gauze, for cover media surface make fruit bat lay eggs and larva adhere to.
Compared with currently available technology, the present invention is easy to obtain each worm state, especially mature larvae and the fruit bat pupa of fruit bat, the fruit bat mature larvae of acquisition and pupa surface cleaning, and pupal cell is complete, without damaged.
Accompanying drawing explanation
Fig. 1 is that the present invention raises tank schematic diagram
Embodiment
Describe the present invention with reference to the accompanying drawings below, it is a kind of preferred embodiment in numerous embodiments of the present invention.
The present embodiment discloses a kind of liquid nutrient medium that uses and has cultivated drosophila larvae and collect the method that separates and obtain in a large number drosophila larvae and pupa, and provides application the method required device.Specifically comprise: obtaining liq medium also saves backup, use fruit bat cage mode to raise into fly; Make the ventilative raising tank of raising drosophila larvae, liquid nutrient medium is added to and raises tank and above medium, lay gauze, raising tank is put into fly cage periodic replacement, obtain fruit bat ovum; Raise aging to drosophila larvae after, with water-washing method wash-out and filter collect mature larva, be placed in food preserving bag; After drosophila larvae is pupated, use scissors that fruit bat pupa is cut together with plastic sack, obtain undamaged fruit bat pupa.Specifically comprise the following steps:
In step 1, use liquid culture based formulas to raise drosophila larvae, fill a prescription as dusty yeast 5g, sucrose 55g, corn flour 51 and water 500g.In step 1, dusty yeast is added in the time that medium is cooled to room temperature again.Make and support fly cage with 100 order nylon wires, the cage hole of side opening one diameter 15cm, by the sleeve with 100 order nylon wires making and hole, cage hole, along stitching, sleeve place tightens with rope.Use the cylindrical transparent plastic can of 300mL as raising tank, open the circular hole that a diameter is about 8cm in the middle of the screw lid of tank mouth, envelope is with 100 order nylon wires, for raising the ventilative of tank.Add two of circular Gauzes in media surface, lay eggs and adhere to larva for fruit bat, spray an a small amount of water with spray bottle every day, to prevent gauze surface desiccation.Make the method that washes with water by mature larvae wash-out and use the mesh screen that 40 order nylon wires are made to filter, removing medium residue, obtaining mature larvae.Fruit bat mature larvae is placed in to food preserving bag and pupates, take off fruit bat pupa together with plastic sheets.
The plastic tank that relates in the present invention, dusty yeast, sucrose, corn flour etc. are all derived from market to be bought, and water is that tap water heating boils.Raise the culture tank of drosophila larvae use as shown in drawings, culture tank is used diameter to be about the cylindrical transparent plastic can that 8cm, height be about 6cm and makes, tank mouth has screw lid, and screw lid central authorities are used hot knife to cut the circular hole that a diameter is about 6cm, uses thermosol gelgun to seal with 100 order nylon wires.In tank, add the about 50mL of liquid nutrient medium, media surface lid is with 2 circular layer gauzes, and uses tweezers that gauze is paved.
Culture medium prescription is dusty yeast 5g, sucrose 55g, corn flour 51 and water 500g.In the time that medium configures, first water heating is boiled, add sucrose and the corn flour of respective amount, after fully mixing, be cooled to normal temperature, then add dusty yeast stirring and evenly mixing; It is for subsequent use that the medium configuring is put into 6 ℃ of conventional refrigerators, when use, from refrigerator, takes out, and after preheating, uses.
Making the length of sides with 100 order nylon wires is the fly cage main body of cube recuperating of 50 centimetres; Support the cage hole of fly cage main body side opening one diameter 15cm, by the sleeve with 100 order nylon wires making and hole, cage hole, along stitching, sleeve place tightens with rope; In fly cage, place approximately 1000 of Drosophila melanogaster adults.Within every 12 hours, change and raise tank and spray a small amount of water with spraying bottle, to prevent gauze surface desiccation.At 25 ℃, raise after 4 days, in plastic tank, occur that a small amount of fruit bat pupates, most of larva enters the aged stage; Feed in plastic tank is poured in basin together with gauze, put the warm water of approximately 25 ℃ in basin, concussion comes off drosophila larvae and takes out gauze, uses the mesh screen that 40 order nylon wires are made to filter, and removes medium residue.Fruit bat mature larvae is placed in to food preserving bag, draws the larger water droplet on larva surface with blotting paper, sack place does not seal bundle; After 2 days, the drosophila larvae in bag is pupated, and uses little scissors that fruit bat pupa is cut together with plastic sack, obtains undamaged fruit bat pupa.
Use method of the present invention, can obtain not damaged fruit bat pupa every day more than 300, also can as required, increase the one-tenth fly quantity in fruit bat cage, obtain more drosophila larvaes, required to meet.
By reference to the accompanying drawings the present invention is exemplarily described above; obviously specific implementation of the present invention is not subject to the restrictions described above; as long as the various improvement that adopted method design of the present invention and technical scheme to carry out; or directly apply to other occasion without improvement, all within protection scope of the present invention.
Claims (10)
1. a method of utilizing liquid nutrient medium breeding fruit bat, is characterized in that, comprises the steps:
(1) obtaining liq medium;
(2) use fruit bat cage mode to raise into fly;
(3) breeding grub obtain fly blow:
(3-1) make the raising tank of raising drosophila larvae;
(3-2) liquid nutrient medium is added to raising tank;
(3-3) raising tank is put into fly cage;
(3-4) obtain fruit bat ovum;
(4) raise aging to drosophila larvae after, wash-out and filter collect mature larva;
(5) be placed in bag, after drosophila larvae is pupated, fruit bat pupa cut together with bag, obtain undamaged fruit bat pupa.
2. the method for utilizing liquid nutrient medium breeding fruit bat as claimed in claim 1, is characterized in that, step (1) comprising:
(1-1) obtaining liq medium: the component of medium and proportioning are: 5 parts of dusty yeasts: 55 parts of sucrose: 51 parts of corn flour: 500 parts, water;
(1-2) preservation of medium and for subsequent use.
3. the method for utilizing liquid nutrient medium breeding fruit bat as claimed in claim 2, is characterized in that, step (1-1) is in the time that medium configures, first water heating is boiled, add sucrose and the corn flour of respective amount, after fully mixing, be cooled to normal temperature, then add dusty yeast stirring and evenly mixing; And/or, in step (1-2), the medium configuring is put into 6 ℃ of conventional refrigerators for subsequent use.
4. the method for utilizing liquid nutrient medium breeding fruit bat as described in any one in claim 1-3, is characterized in that, step (2) comprising:
(2-1) fly cage is supported in design;
(2-2) in fly cage, place fruit bat adult.
5. the method for utilizing liquid nutrient medium breeding fruit bat as claimed in claim 4, it is characterized in that, in step (2-1), making the length of sides with 100 order nylon wires is the fly cage main body of cube recuperating of 50 centimetres, support the cage hole of fly cage main body side opening one diameter 15cm, by with 100 order nylon wires make sleeve and hole, cage hole along stitching, sleeve place tightens with rope; In step (2-2), in fly cage, place approximately 1000 of Drosophila melanogaster adults.
6. the method for utilizing liquid nutrient medium breeding fruit bat as described in any one in claim 1-5, is characterized in that, in step (3):
(3-1) in, make the ventilative raising tank of raising drosophila larvae: in volume is the cylindrical transparent plastic can of 300mL, raise, open the circular hole that a diameter is about 6cm in the middle of the screw lid of tank mouth, envelope is with 100 order nylon wires, for raising the ventilative of tank;
(3-2) liquid nutrient medium is added to and raises tank and above medium, lay gauze: raise in tank and put into the about 50mL of above-mentioned medium, then add two of circular Gauzes in media surface, lay eggs and adhere to larva for fruit bat;
(3-3) raising tank is put into fly cage periodic replacement: raising tank is put into fly cage, within every 12 hours, regularly take out and cover tightly with above-mentioned ventilative screw lid, and changing the raising tank that contains fresh culture; Open screw lid every day, with a small amount of water of spray bottle sprinkling, to prevent gauze surface desiccation;
(3-4) obtain fruit bat ovum.
7. the method for utilizing liquid nutrient medium breeding fruit bat as described in any one in claim 1-6, is characterized in that, in step (4):
Collect mature larva with water-washing method wash-out filtration; And/or,
Raise after 4 days, raise in tank and occurred that a small amount of fruit bat pupates, most of larva enters the aged stage; The feed of raising in tank is poured in basin together with gauze, put the warm water of approximately 25 ℃ in basin, concussion comes off drosophila larvae and takes out gauze, uses the mesh screen that 40 order nylon wires are made to filter, and removes medium residue.
8. the method for utilizing liquid nutrient medium breeding fruit bat as described in any one in claim 1-7, is characterized in that, in step (5): be placed in food preserving bag, draw the larger water droplet on larva surface with blotting paper, sack place does not seal bundle; After 2 days, in bag, drosophila larvae is pupated, and uses scissors that fruit bat pupa is cut together with plastic sack, obtains undamaged fruit bat pupa.
9. as described in claim 1-8, utilize the device of liquid nutrient medium breeding fruit bat method, it is characterized in that, it is for raising tank, comprise nylon wire, spiral cover and tank body, wherein, tank body is cylindrical transparent plastic can, spiral cover is arranged on the tank mouth of tank body, and nylon wire is arranged on the circular hole of spiral cover central authorities.
10. utilize as claimed in claim 9 the device of liquid nutrient medium breeding fruit bat method, it is characterized in that, described cylindrical transparent plastic can diameter is about 8cm, height is about 6cm, described circular hole is the circular hole that the central diameter that uses hot knife to cut of screw lid is about 6cm, and it adopts thermosol gelgun to seal with nylon wire described in 100 orders; And/or, also comprise 2 layers of circular gauze, for cover media surface make fruit bat lay eggs and larva adhere to.
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| CN105028345A (en) * | 2015-09-08 | 2015-11-11 | 山东省农业科学院植物保护研究所 | Breeding method for propylea japonica |
| CN105284744A (en) * | 2015-11-18 | 2016-02-03 | 遵义医学院 | Incubation method for Drosophila larvae |
| CN105746440A (en) * | 2016-03-10 | 2016-07-13 | 山西省农业科学院植物保护研究所 | Artificial feeding device and artificial feeding method for oriental fruit moth |
| CN105766812A (en) * | 2016-04-07 | 2016-07-20 | 云南省农业科学院农业环境资源研究所 | Method for breeding Drosophila suzukii Matsumura in captivity |
| CN106417189A (en) * | 2016-09-28 | 2017-02-22 | 天津拓华生物科技有限责任公司 | Method for breeding fruit flies in large scale and obtaining fruit fly larvae in concentrated mode |
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| CN110742023A (en) * | 2019-10-25 | 2020-02-04 | 山东省葡萄研究院 | Breeding device and breeding method for fruit fly pupa parasitic wasps |
| CN111316959A (en) * | 2020-03-25 | 2020-06-23 | 广西壮族自治区农业科学院 | Indoor propagation method for bactrocera dorsalis |
| CN111543392A (en) * | 2020-04-27 | 2020-08-18 | 浙江大学 | Method and device for large-scale raising of fruit fly parasitic wasps |
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| CN105284744A (en) * | 2015-11-18 | 2016-02-03 | 遵义医学院 | Incubation method for Drosophila larvae |
| CN105746440B (en) * | 2016-03-10 | 2018-10-30 | 山西省农业科学院植物保护研究所 | The artificial feeding device and method of oriental fruit months |
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| CN106417189A (en) * | 2016-09-28 | 2017-02-22 | 天津拓华生物科技有限责任公司 | Method for breeding fruit flies in large scale and obtaining fruit fly larvae in concentrated mode |
| CN107594123A (en) * | 2017-10-30 | 2018-01-19 | 山西大学 | A kind of feed and production method for delaying the drosophila strains speed of growth |
| CN107594123B (en) * | 2017-10-30 | 2020-08-04 | 山西大学 | Feed for delaying growth speed of fruit fly strain and production method thereof |
| CN110742023A (en) * | 2019-10-25 | 2020-02-04 | 山东省葡萄研究院 | Breeding device and breeding method for fruit fly pupa parasitic wasps |
| CN110742023B (en) * | 2019-10-25 | 2021-11-26 | 山东省农业科学院植物保护研究所 | Breeding device and breeding method for fruit fly pupa parasitic wasps |
| CN110692601A (en) * | 2019-11-12 | 2020-01-17 | 安徽师范大学 | Method for breeding aphelesia crenulata by using drosophila melanogaster to replace drosophila suzukii |
| CN111316959A (en) * | 2020-03-25 | 2020-06-23 | 广西壮族自治区农业科学院 | Indoor propagation method for bactrocera dorsalis |
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