CN1973619A - Industrial method of culturing housefly larva - Google Patents
Industrial method of culturing housefly larva Download PDFInfo
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- CN1973619A CN1973619A CNA2006101242249A CN200610124224A CN1973619A CN 1973619 A CN1973619 A CN 1973619A CN A2006101242249 A CNA2006101242249 A CN A2006101242249A CN 200610124224 A CN200610124224 A CN 200610124224A CN 1973619 A CN1973619 A CN 1973619A
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- common house
- fly
- fly grub
- grub
- housefly
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The industrial method of culturing housefly larva includes the following steps: 1. setting adsorptive matter to the container for culturing housefly larva; 2. injecting to the adsorptive matter the liquid culture medium comprising bran 5-10 wt%, whole milk powder 5-10 wt%, brown sugar 5-8 wt%, carrot juice 5- 15 wt% and water for the rest and with probiotics; 3. spreading housefly egg to the surface of adsorptive matter and incubating the egg into housefly larva; 4. cultivating for 4-10 days; and 5. separating housefly larva with screen. The said housefly larva culturing process has high bran utilizing rate and lowered cost, and is suitable for automation of housefly larva culturing.
Description
Technical field
The present invention relates to use the biotechnology of culture fluid large-scale culture common house-fly grub.
Background technology
Housefly (Musca domestica) belongs to Insecta, diptera, Aristocera, tame Nuscidae, it is good animal protein feed, have advantages such as fertility is strong, life cycle short, be easy to intensive production, feed resource is abundant, classified as in the world first of the novel protein resource.Housefly is a kind of that the most of area of China is the most common, quantity is maximum.The automaticity of China housefly breed at present is low, needs the large-scale production of a large amount of manpowers ability, has restricted the industrialization utilization of housefly.
In the housefly production process, the cultivation of common house-fly grub is an of paramount importance stage.The common house-fly grub medium that adopts mainly contains two big classes at present, and a class is fowl and animal excrements such as chicken manure, pig manure; Another kind of is agricultural byproducts such as wheat bran, bean dregs and tankage.In suitability for industrialized production, use fowl and animal excrements such as chicken manure, pig manure etc. as medium, the medium instability, and be easy to sneak into harmful bacterium, be unfavorable for output and the later use of housefly; And common house-fly grub is very low to the nutrient utilization of solid culture mediums such as wheat bran, bean dregs, and the wheat bran of cultivating behind the common house-fly grub can not be edible by other fowl poultrys again, so use the cost of wheat bran cultivation common house-fly grub higher, is unfavorable for the industrialization of housefly.
Summary of the invention
The objective of the invention is to propose a kind of method of using the culture fluid industrialization to cultivate common house-fly grub, with the large-scale culture common house-fly grub.
The present invention adopts following measure to realize:
(1) sticks in the bottom of the culture vessel of common house-fly grub or place adsorbability material;
(2) on the adsorbability material of culture vessel, inject liquid nutrient medium, the material of components by weight percent for preparing described liquid nutrient medium is as follows: wheat bran 5-10%, whole milk powder 5～10%, brown sugar 5～8%, carrot juice 5～15%, surplus is a water, and adds probio in medium;
(3) tame fly blow is dispersed evenly to the adsorbability material surface that is adsorbed with liquid nutrient medium, inoculum concentration be generally 40000 ovum/square metre, make ovum hatch into common house-fly grub;
(4) cultivate common house-fly grub having on the adsorbability material surface of sufficient liquid nutrient medium, between culture period constantly at adsorbability material surface liquid make-up medium, the specification that amount of replenishing every day and number of times are got food situation and culture vessel according to housefly is generally twice of every day;
(5) cultivate after 4～10 days, utilize screen cloth that common house-fly grub is separated.
Common house-fly grub culture vessel of the present invention can be vinyl disc or metal dish, also can be cement pit, and it highly is generally 20cm～30cm, and length and width are made by oneself according to needs of production.
The adsorbability material that the present invention is stained with or places in the bottom of common house-fly grub culture vessel is the filling absorption carrier as liquid nutrient medium.Described adsorbability material can be sponge, cotton, plastic foam or other can adsorb the adsorbability material of culture fluid, can attach to or just be positioned over the bottom of culture vessel.After adding liquid nutrient medium, adsorbability material absorption medium, for common house-fly grub get food and life provides nutriment.
The liquid nutrient medium of raising common house-fly grub provided by the present invention, its component includes wheat bran, whole milk powder, brown sugar and carrot juice.Wheat bran can sieve through 15 purpose screen clothes, and is then that each component materials is standby after the agitator stir process, the medium non-sterile of making or all could in 20 minutes through 100 ℃ of sterilizations.
The present invention adds probio in medium, the growth of probio helps controlling the pollution that may occur in the common house-fly grub incubation.The kind of probio is a lot, and bacterial classification and the employed bacterial classification of feed level microbe additive mentioned in The Ministry of Agriculture of the People's Republic of China, MOA's bulletin (No. 161) " fertilizer data registration request " all can use.The main kind that the present invention uses comprises that (bacterial classification can obtain from Chinese common micro-organisms culture presevation administrative center S. cervisiae (Saccharomyces cerevisiae), be numbered 2.1), (bacterial classification can obtain from Chinese common micro-organisms culture presevation administrative center bacillus licheniformis (Bacillus licheniformis), be numbered 1.91) and enterobacter cloacae (Enterobacter cloacae) (bacterial classification can obtain from Chinese common micro-organisms culture presevation administrative center, is numbered 1.57).Probio can add in the medium simultaneously with tame fly blow, also can add probio earlier, and cultivation adds tame fly blow after one to a couple of days again.
Adopt cultural method of the present invention to use liquid nutrient medium, can significantly lower common house-fly grub and cultivate required solid feed one wheat bran, the nutrient utilization height, and can reduce subsequent treatment operation (the particularly separation of common house-fly grub), help the housefly larva cultivation process automation, reduce the cost of large-scale culture common house-fly grub.
Embodiment
The following example is that two kinds of practical operations of the present invention are further specified the restriction of the present invention of should not opposing.
Embodiment one
Cultivate the bottom pad lastblock sponge carrier (high 5cm, long 80cm, wide 40cm) of vinyl disc (height 25cm, length and width are 80cm, 40cm respectively) in common house-fly grub; (culture fluid contains 8% wheat bran, 5% whole milk powder, 5% brown sugar, 5% carrot juice to add the 2L culture fluid in every culture plate on sponge carrier; Through 100 ℃ of sterilizations 20 minutes); In different culture plates, add the S. cervisiae of cultivating 24 hours (120rpm, 25 ℃) through shaking table then respectively; Simultaneously with tame fly blow uniformly dispersing on the surface of sponge block, inoculum concentration be 40000 ovum/square metre; Under 25～30 ℃ of temperature, ovum is hatched into larva; At every turn add 100ml to the above-mentioned culture fluid twice of sponge block surface sprinkling every day, stirs.
Common house-fly grub utilizes the continuous growing of the nutrition of liquid nutrient medium.Cultivation is after 3 days down at 25～30 ℃, and common house-fly grub develops into mature larva, uses 15 purpose screen clothes that sponge block, medium are separated with larva, obtains 1.48 kilograms of common house-fly grubs.
Embodiment two
Cultivate the bottom pad of vinyl disc (height 25cm, length and width are 80cm, 40cm respectively) in common house-fly grub and go up plastic foam carrier (high 5cm, long 80cm, wide 40cm); (culture fluid contains 5% wheat bran, 8% whole milk powder, 6% brown sugar, 10% carrot juice to add the 2L culture fluid in every culture plate on the plastic foam carrier; Through 100 ℃ of sterilizations 20 minutes); In different culture plates, add the bacillus licheniformis of cultivating 24 hours (120rpm, 25 ℃) through shaking table then respectively; Simultaneously with tame fly blow uniformly dispersing on the surface of sponge block, inoculum concentration be 40000 ovum/square metre; Under 25～30 ℃ of temperature, ovum is hatched into larva; At every turn add 100ml to the above-mentioned culture fluid twice of sponge block surface sprinkling every day, stirs.
Common house-fly grub utilizes the continuous growing of the nutrition of liquid nutrient medium.Cultivation is after 3 days down at 25～30 ℃, and common house-fly grub develops into mature larva, uses 15 purpose screen clothes that sponge block, medium are separated with larva, obtains 1.28 kilograms of common house-fly grubs.
Embodiment three
Cultivate the bottom pad of vinyl disc (height 25cm, length and width are 80cm, 40cm respectively) in common house-fly grub and go up plastic foam carrier (high 5cm, long 80cm, wide 40cm); (culture fluid contains 10% wheat bran, 10% whole milk powder, 8% brown sugar, 12% carrot juice to add the 2L culture fluid in every culture plate on the plastic foam carrier; Through 100 ℃ of sterilizations 20 minutes); In different culture plates, add the enterobacter cloacae of cultivating 24 hours (120rpm, 25 ℃) through shaking table then respectively; Simultaneously with tame fly blow uniformly dispersing on the surface of sponge block, inoculum concentration be 40000 ovum/square metre; Under 25～30 ℃ of temperature, ovum is hatched into larva; At every turn add 100ml to the above-mentioned culture fluid twice of sponge block surface sprinkling every day, stirs.
Common house-fly grub utilizes the continuous growing of the nutrition of liquid nutrient medium.Cultivation is after 3 days down at 25～30 ℃, and common house-fly grub develops into mature larva, uses 15 purpose screen clothes that sponge block, medium are separated with larva, obtains 1.56 kilograms of common house-fly grubs.
Claims (3)
1. the method for common house-fly grub is cultivated in an industrialization, it is characterized in that comprising following technical measures:
(1) sticks in the bottom of the culture vessel of common house-fly grub or place adsorbability material;
(2) on the adsorbability material of culture vessel, inject liquid nutrient medium, the material of components by weight percent for preparing described liquid nutrient medium is as follows: wheat bran 5-10%, whole milk powder 5～10%, brown sugar 5～8%, carrot juice 5～15%, surplus is a water, and adds probio in medium;
(3) tame fly blow is dispersed evenly to the adsorbability material surface that is adsorbed with liquid nutrient medium, makes ovum hatch into common house-fly grub;
(4) cultivate common house-fly grub having on the adsorbability material surface of sufficient liquid nutrient medium, between culture period constantly at adsorbability material surface liquid make-up medium;
(5) cultivate after 4～10 days, utilize screen cloth that common house-fly grub is separated.
2. the method for common house-fly grub is cultivated in industrialization according to claim 1, and stick the bottom that it is characterized in that described common house-fly grub culture vessel or the adsorbability material placed is sponge, cotton or plastic foam.
3. the common house-fly grub method is cultivated in industrialization according to claim 1, it is characterized in that described probio kind is one or more combinations in S. cervisiae (Saccharomyces cerevisiae), bacillus licheniformis (Bacillus licheniformis) or the enterobacter cloacae (Enterobacter cloacae).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006101242249A CN1973619B (en) | 2006-12-15 | 2006-12-15 | Industrial method of culturing housefly larva |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006101242249A CN1973619B (en) | 2006-12-15 | 2006-12-15 | Industrial method of culturing housefly larva |
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| Publication Number | Publication Date |
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| CN1973619A true CN1973619A (en) | 2007-06-06 |
| CN1973619B CN1973619B (en) | 2010-08-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2006101242249A Expired - Fee Related CN1973619B (en) | 2006-12-15 | 2006-12-15 | Industrial method of culturing housefly larva |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101156565B (en) * | 2007-11-13 | 2010-06-23 | 广东省昆虫研究所 | A method for separating housefly grub |
| CN101317559B (en) * | 2008-07-16 | 2010-12-08 | 广东省昆虫研究所 | Sharp separation method for housefly larvas |
| CN102550487A (en) * | 2011-01-21 | 2012-07-11 | 中国人民解放军第四五四医院 | Production method of aseptic maggots |
| CN102599389A (en) * | 2012-04-09 | 2012-07-25 | 中山大学 | Feed for larvae of houseflies and preparation method thereof |
| CN102630838A (en) * | 2012-04-27 | 2012-08-15 | 中国计量学院 | Preparation scheme and method of citrus fruit fly larva feed |
| CN103583901A (en) * | 2013-11-07 | 2014-02-19 | 周辛平 | Fly and maggot cultivation method and high protein feed adopting same |
| CN103843728A (en) * | 2014-03-14 | 2014-06-11 | 汪岩屹 | Method and device for propagating fruit flies by utilizing liquid culture medium |
| CN105104308A (en) * | 2015-07-22 | 2015-12-02 | 青岛农业大学 | Free-living marine nematode liquid culture solution and application thereof |
-
2006
- 2006-12-15 CN CN2006101242249A patent/CN1973619B/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101156565B (en) * | 2007-11-13 | 2010-06-23 | 广东省昆虫研究所 | A method for separating housefly grub |
| CN101317559B (en) * | 2008-07-16 | 2010-12-08 | 广东省昆虫研究所 | Sharp separation method for housefly larvas |
| CN102550487A (en) * | 2011-01-21 | 2012-07-11 | 中国人民解放军第四五四医院 | Production method of aseptic maggots |
| CN102599389A (en) * | 2012-04-09 | 2012-07-25 | 中山大学 | Feed for larvae of houseflies and preparation method thereof |
| CN102630838A (en) * | 2012-04-27 | 2012-08-15 | 中国计量学院 | Preparation scheme and method of citrus fruit fly larva feed |
| CN103583901A (en) * | 2013-11-07 | 2014-02-19 | 周辛平 | Fly and maggot cultivation method and high protein feed adopting same |
| CN103843728A (en) * | 2014-03-14 | 2014-06-11 | 汪岩屹 | Method and device for propagating fruit flies by utilizing liquid culture medium |
| CN103843728B (en) * | 2014-03-14 | 2015-08-19 | 汪岩屹 | A kind of method and device utilizing liquid nutrient medium breeding fruit bat |
| CN105104308A (en) * | 2015-07-22 | 2015-12-02 | 青岛农业大学 | Free-living marine nematode liquid culture solution and application thereof |
| CN105104308B (en) * | 2015-07-22 | 2016-03-09 | 青岛农业大学 | A kind of Free-living amoeba liquid medium and application thereof |
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| Publication number | Publication date |
|---|---|
| CN1973619B (en) | 2010-08-11 |
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Address after: 510260 No. 105 West Xingang Road, Guangdong, Guangzhou Patentee after: Guangdong Institute of biological resources application Address before: 510260 No. 105 West Xingang Road, Guangdong, Guangzhou Patentee before: Guangdong Entomological Institute |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100811 Termination date: 20201215 |
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| CF01 | Termination of patent right due to non-payment of annual fee |