CN100494121C - Preparation method of biofertilizer - Google Patents

Preparation method of biofertilizer Download PDF

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Publication number
CN100494121C
CN100494121C CNB2006101323655A CN200610132365A CN100494121C CN 100494121 C CN100494121 C CN 100494121C CN B2006101323655 A CNB2006101323655 A CN B2006101323655A CN 200610132365 A CN200610132365 A CN 200610132365A CN 100494121 C CN100494121 C CN 100494121C
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China
Prior art keywords
wheat bran
culture
feritlizer
bio
fly maggot
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CNB2006101323655A
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Chinese (zh)
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CN100999417A (en
Inventor
韩日畴
陈镜华
曹莉
刘小芬
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广东省昆虫研究所
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Publication of CN100999417A publication Critical patent/CN100999417A/en
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Publication of CN100494121C publication Critical patent/CN100494121C/en

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Abstract

This invention provides a method of using housefly larvae and life-profit-germ to handle bran for preparation of biological fertilizer. It includes the following process: (1) adding life-profit-germ into bran for culture; (2) put the bran after cultivation in housefly larvae breed plate, add housefly eggs; (3) In the 20-30 deg C for 3 to 5 days, isolate housefly larvae; (4) seal the cultivation material after isolating housefly larvae for fermentation to kill the remaining frogeye maggot, then obtain microbial fertilizer; (5) drying and packaging.

Description

A kind of preparation method of bio-feritlizer
Technical field
The present invention relates to a kind of method of utilizing Musca domestica larva and probiotic bacterium to handle wheat bran acquisition bio-feritlizer.
Background technology
Housefly (Musca domestica) is to belong to Insecta, Diptera, Aristocera, tame Nuscidae, has that reproductivity is strong, life cycle short, feed resource enriches, is easy to advantages such as intensive production, is listed in the world first of the novel protein resource.
In Musca domestica larva (fly maggot) culturing process, agricultural byproducts such as general using wheat bran are as substratum.Musca domestica larva is very low to the nutrient utilization of substratum such as wheat bran, rice bran, so the substratum defective material behind the cultivation fly maggot still has higher nutrient contg, but these substratum defective materials can not be re-used as the feed of other animal, and these substratum defective materials are dealt with improperly, may pollute the source.If these substratum defective materials of energy have not only solved how to dispose the problem of these defective materials as fertilizer, and lower the whole cost that fly maggot is cultivated, helped the industrialization utilization of fly maggot.
Summary of the invention
The purpose of this invention is to provide and a kind ofly handle the method that the agricultural byproducts wheat bran obtains bio-feritlizer with Musca domestica larva and probiotic bacterium, this method makes the substratum defective material of cultivating behind the fly maggot obtain utilizing and obtaining bio-feritlizer when obtaining high-quality protein-fly maggot.The present invention develops new bio-feritlizer a kind of feasible thinking is provided for the value added applications that solves agricultural byproducts, the cost that lowers the housefly industrialization.
The present invention adopts following measure to realize: (1) adds probiotic bacterium in the wheat bran culture and cultivates; (2) the wheat bran lay after will cultivating adds tame fly blow in the Musca domestica larva rearing tray; (3) after cultivating 3~5 days under 20-30 ℃, isolate the Musca domestica larva of having cultivated out; (4) will isolate culture material sealing and fermenting again after the Musca domestica larva, and kill the unsegregated fly maggot of residue and can gather in the crops and obtain microbial fertilizer; (5) drying, the packing of the microbial fertilizer that obtains.
In order to separate the convenience of Musca domestica larva in the last handling process, employed wheat bran is preferably through 15 eye mesh screens.The kind of available probiotic bacterium of the present invention is a lot, and bacterial classification and the employed bacterial classification of feed level microbe additive mentioned in The Ministry of Agriculture of the People's Republic of China, MOA's bulletin (No. 161) " fertilizer data registration request " all can use.The probiotic bacterium kind of recommending to use comprises S. cervisiae (Saccharomyces cerevisiae), bacillus licheniformis (Bacillus licheniformis) or enterobacter cloacae (Enterobacter cloacae).Probiotic bacterium generally passes through liquid nutrient medium (LB) shaking table concussion (120rpm, 25 ℃) and cultivated 24-48 hour, adds probiotics bacterial liquid then in the wheat bran culture, and preferably sealing was cultivated 1-2 days.The amount that adds probiotics bacterial liquid is generally the 1-10% (weight ratio) of the wheat bran weight in the wheat bran culture.Described wheat bran culture is the mixture of wheat bran and water.
But the wheat bran lay after probiotic bacterium is handled is in the fly maggot breeding dish, and the thickness of lay is generally about 5cm, and fly blow is dispersed in the wheat bran surface, inoculum size be 20000~25000 ovum/square metre.Cultivated 3-5 days down at 20-30 ℃, just can utilize the negative phototaxis of Musca domestica larva, allow Musca domestica larva get over 20 purpose screen clothes, the realization fly maggot separates with wheat bran.Culture material behind results fly maggot sealing and fermenting 1-3 days again after leavening temperature reaches 50 ℃, is killed unsegregated fly maggot.The bio-feritlizer that is obtained is dry and packing according to ordinary method.
Wheat bran culture (bio-feritlizer) through different probiotic bacteriums and Musca domestica larva processing can be distinguished packing according to the method described above, also the wheat bran culture (bio-feritlizer) through different probiotic bacteriums and Musca domestica larva processing can be mixed in proportion packing.
The present invention is in conjunction with Musca domestica larva (fly maggot) culture process, by in the wheat bran culture, adding probiotic bacterium, utilize the nutrition of the growth conversion wheat bran culture of probiotic bacterium, in order to cultivate Musca domestica larva (fly maggot), after results high-quality protein-fly maggot, continuation is carried out fermentative processing to the substratum defective material that is rich in nutrition, and obtains biological organic fertilizer.
The biological organic fertilizer that the present invention obtained is Vandyke brown, and quality is soft.Sampling analysis after drying, water content is lower than 20%, and organic content is greater than 65% (with dry weight basis), and total nutrient (nitrogen, phosphorus, potassium) content (with dry weight basis) is greater than 9%, and potential of hydrogen is 6.5-7.5, thalline quantity is greater than 300,000,000/gram.This is a kind of good bio-feritlizer.
Embodiment
Following example is to the further specifying of actually operating of the present invention, and should not be used as limitation of the present invention.
Example one:
In fermentor tank, use liquid nutrient medium (LB substratum) to cultivate S. cervisiae respectively; In fermentation vat, the above-mentioned bacterium liquid of 30L is added in the 300 kilograms of wheat brans and 900 premium on currency through 15 eye mesh screens, behind the thorough mixing, use diaphragm seal fermentation 1 day; Wheat bran after the fermentation is spread out, form the cultivation bed of material about 5cm; Clean fly blow interspersed among cultivates on the charge level, inoculum density be 20000~25000 ovum/square metre, the fly blow hatching is larva; Cultivated 3.5 days down for 26 ℃ in room temperature, fly maggot utilizes the nutritional development of culture material to become mature larva.
Culture material is tiled in the big screen cloth of 20 purposes, forms the bed of material about 5cm, on be placed under the fluorescent lamp because fly maggot has negative phototaxis, fly maggot gets over screen cloth voluntarily and enters dark bottom space, this process about 12h consuming time.The fly maggot of results is 148 kilograms.
Behind the results fly maggot, the substratum defective material was put into the fermentation vat sealing and fermenting 2 days again, and after the center material layer temperature surpassed 50 ℃, the residue fly maggot can be all dead, and defective material is fully become thoroughly decomposed simultaneously, is lower than 20% with warm air drying to water content, and obtaining bio-feritlizer is 286 kilograms.
Example two:
In fermentor tank, use liquid nutrient medium (LB substratum) to cultivate bacillus licheniformis; In fermentation vat, go up 30 and to state bacterium liquid and add respectively in the 300 kilograms of wheat brans and 900 premium on currency through 15 eye mesh screens, behind the thorough mixing, use diaphragm seal fermentation 1 day; Wheat bran after the fermentation is spread out, form the cultivation bed of material about 5cm; Clean fly blow interspersed among cultivates on the charge level, inoculum density be 20000~25000 ovum/square metre, the fly blow hatching is larva; Cultivated 3.5 days down for 26 ℃ in room temperature, fly maggot utilizes the nutritional development of culture material to become mature larva.
Culture material is tiled in the big screen cloth of 20 purposes, forms the bed of material about 5cm, on be placed under the fluorescent lamp because fly maggot has negative phototaxis, fly maggot gets over screen cloth voluntarily and enters dark bottom space, this process about 12h consuming time.The fly maggot of results is respectively 159 kilograms.
Behind the results fly maggot, the substratum defective material was put into the fermentation vat sealing and fermenting 2 days again, and after the center material layer temperature surpassed 50 ℃, the residue fly maggot can be all dead, and defective material is fully become thoroughly decomposed simultaneously, is lower than 20% with warm air drying to water content, and obtaining bio-feritlizer is 298 kilograms.
Example three:
In fermentor tank, use liquid nutrient medium (LB substratum) to cultivate enterobacter cloacae; In fermentation vat, the above-mentioned bacterium liquid of 30L is added respectively in the 300 kilograms of wheat brans and 900 premium on currency through 15 eye mesh screens, behind the thorough mixing, use diaphragm seal fermentation 1 day; Wheat bran after the fermentation is spread out, form the cultivation bed of material about 5cm; Clean fly blow interspersed among cultivates on the charge level, inoculum density be 20000~25000 ovum/square metre, the fly blow hatching is larva; Cultivated 3.5 days down for 26 ℃ in room temperature, fly maggot utilizes the nutritional development of culture material to become mature larva.
Culture material is tiled in the big screen cloth of 20 purposes, forms the bed of material about 5cm, on be placed under the fluorescent lamp because fly maggot has negative phototaxis, fly maggot gets over screen cloth voluntarily and enters dark bottom space, this process about 12h consuming time.The fly maggot of results is respectively 139 kilograms.
Behind the results fly maggot, the substratum defective material was put into the fermentation vat sealing and fermenting 2 days again, and after the center material layer temperature surpassed 50 ℃, the residue fly maggot can be all dead, and defective material is fully become thoroughly decomposed simultaneously, is lower than 20% with warm air drying to water content, and obtaining bio-feritlizer is 281 kilograms.
The biological organic fertilizer that aforesaid method obtained is Vandyke brown, and quality is soft.Sampling analysis after drying, water content is lower than 20%, and organic content is greater than 65% (with dry weight basis), and total nutrient (nitrogen, phosphorus, potassium) content (with dry weight basis) is greater than 9%, and potential of hydrogen is 6.5-7.5, thalline quantity is greater than 300,000,000/gram.This is a kind of good bio-feritlizer.

Claims (3)

1. the preparation method of a bio-feritlizer is characterized in that may further comprise the steps:
(1) probiotic bacterium is added in the wheat bran culture cultivates; (2) the wheat bran lay after will cultivating adds tame fly blow in housefly (Muscadomestica) larva raising dish; (3) after cultivating 3~5 days under 20~30 ℃, separate Musca domestica larva; (4) raise culture material sealing and fermenting again behind the Musca domestica larva, kill the unsegregated fly maggot of residue; (5) drying, the packing of the microbial fertilizer that obtains.
2. the preparation method of bio-feritlizer according to claim 1, it is characterized in that described probiotic bacterium is earlier through the liquid nutrient medium cultivation, add in the wheat bran culture again and cultivate, the weight of the probiotics bacterial liquid of adding is the 1-10% of wheat bran weight in the wheat bran culture.
3. the preparation method of bio-feritlizer according to claim 1 and 2 is characterized in that described probiotic bacterium kind is S. cervisiae (Saccharomyces cerevisiae), bacillus licheniformis (Bacillus licheniformis) or enterobacter cloacae (Enterobacter cloacae).
CNB2006101323655A 2006-12-28 2006-12-28 Preparation method of biofertilizer CN100494121C (en)

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CN101879517B (en) * 2010-06-13 2013-09-18 中山大学 Method for processing utensil rubbish by Chrysomya megacephala maggot and material formula
CN101913742B (en) * 2010-07-08 2012-05-23 周雨珺 Method for biologically treating and transforming blue algae by using insect larvae
CN104692972B (en) * 2015-03-03 2017-06-27 中国烟草总公司广东省公司 One grows cotton biological activity organic fertilizer special and its application
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CN105218217A (en) * 2015-08-21 2016-01-06 成都圣灵生物科技有限公司 A kind of Dictyophora indusiata Cultivation liquid that topdresses
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CN105218273A (en) * 2015-08-21 2016-01-06 成都圣灵生物科技有限公司 A kind of preparation method of morel cultivation soil-repairing agent
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CN105218191A (en) * 2015-08-21 2016-01-06 成都圣灵生物科技有限公司 A kind of golden mushroom plantation liquid that topdresses
CN105218272A (en) * 2015-08-21 2016-01-06 成都圣灵生物科技有限公司 A kind of Dictyophora indusiata Cultivation soil-repairing agent
CN106167423A (en) * 2016-06-22 2016-11-30 泰山医学院 A kind of method utilizing fly larvae to convert edible fungi residue production high protein organic fertilizer
CN106478225A (en) * 2016-08-31 2017-03-08 泰山医学院 A kind of preparation method of high protein killing pests and preventing diseases bio-feritlizer
CN107319101B (en) * 2017-06-15 2021-01-01 深圳市博裕家园生物科技有限公司 Fermented antibiotic-free protein source material and preparation method and application thereof
CN107439492A (en) * 2017-09-05 2017-12-08 周辛平 Utilize the serialization maggot cultural method of ferment feed
CN108456025A (en) * 2018-03-23 2018-08-28 新疆金胡杨牧业有限公司 maggot protein biological organic fertilizer and preparation method thereof
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