CN106259221B - A method of raising beet armyworm - Google Patents

A method of raising beet armyworm Download PDF

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Publication number
CN106259221B
CN106259221B CN201610691767.2A CN201610691767A CN106259221B CN 106259221 B CN106259221 B CN 106259221B CN 201610691767 A CN201610691767 A CN 201610691767A CN 106259221 B CN106259221 B CN 106259221B
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beet armyworm
feed
powder
raising
conducive
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CN106259221A (en
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华巧波
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
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  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Fodder In General (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention provides a kind of methods for raising beet armyworm to set the different beet armyworm unit area numbers of animals raised for the different phase of beet armyworm growth, is conducive to its health, safety growth;Feed includes following main component: corn flour, yeast powder, citric acid, sodium benzoate, sucrose, vitamin C, mulberry leaf, angelica powder, cassia seed powder and water;On the basis of traditional beet armyworm feed, it is added to two Chinese medicine of angelica powder and cassia seed powder, it is combined with mulberry leaf, beet armyworm immunity can be improved, to achieve the effect that improve its larva survival rate, percentage of pupation and eclosion rate, through detecting, the beet armyworm raised using feed of the invention, reproductive efficiency can be improved 10% or more.

Description

A method of raising beet armyworm
Technical field
The present invention relates to insects to raise field, particularly, is related to a kind of method for raising beet armyworm.
Background technique
The lepidoptera pests such as beet armyworm (Spodoptera exigua H ü bner) are a kind of primary pests on vegetables, With fulminant, binge, the features such as being also easy to produce drug resistance, (department rose cloud etc. 2009;Liu Xiangyangs etc. 2007;Moulton et Al 2000), cause difficulty of prevention and cure to increase, control cost improves, excessive pesticide residues etc., directly threat food safety, obstruction pair Outer Trade Development, destroying the ecological balance, (road is triumphant to wait 2005;Huang Yingjia 2013).
It establishes beet armyworm strain, optimizes beet armyworm artificial diet technique, a large amount of normal developments, neat and consistent are provided Selected insect source is insect Physiology and biochemistry, the element task of the multinomial research such as entomological genetics and insect toxicology.Insect growth week Phase is short, and population quantity is big, is the best model organism material for carrying out biological study.From the artificial of starting calliphora vomitoria in 1908 After feed formula, the research of insect artificial feeding gradually grows up.Lot of domestic and international scholar carries out insect artificial diet Research, it is especially most with lepidopterous insects.
Chinese invention patent CN201110386742.9 discloses a kind of feed formula of beet armyworm, and the Feed Material is easy It obtains, formula of artificial feed for beet armyworm at low cost, can largely breeding beet armyworm, but in order to further increase beet armyworm Reproductive efficiency, it is necessary to study a kind of new formula of artificial feed for beet armyworm.
Summary of the invention
It is an object of the present invention to study a kind of method for raising beet armyworm.
A method of raising beet armyworm follows the steps below:
A, will produce, which has the ovum paper of Beet Armyworm Eggs to be cut into the small scraps of paper with scissors, is placed in sterile petri dish, to ovum blackening Afterwards, it is put into a small amount of feed, abandons ovum paper after larva hatches completely;
B, 1-3 age raises in culture dish always, and larva 250-300 head, is put into feed in each culture dish;
C, after larva length to 3 ages, larva 8-10 head in each culture dish is allocated larva to other culture dishes with writing brush In, it is put into feed, to long to 4 age ends removing excreta, adds feed, just feed is eaten up when supporting prepupal period, and is allowed to It pupates in culture dish.
Preferably, the condition of the raising beet armyworm are as follows: 26 ± 1 DEG C of temperature, photoperiod illumination 14h/ dark 10h, Relative humidity is 65-70%R.H..
Preferably, the feed is the feed for being conducive to improve beet armyworm reproductive efficiency.
Preferably, the described feed for being conducive to improve beet armyworm reproductive efficiency, including following weight percent at Point:
Corn flour 15-25%
Yeast powder 5-8%
Citric acid 0.1-0.3%
Sodium benzoate 2-5%
Sucrose 3-5%
Vitamin C 0.5-1%
Mulberry leaf 3-5%
Angelica powder 1-2%
Cassia seed powder 0.3-0.6%
Water surplus.
Preferably, the described feed for being conducive to improve beet armyworm reproductive efficiency, including following weight percent at Point:
Corn flour 15-20%
Yeast powder 6-8%
Citric acid 0.2-0.3%
Sodium benzoate 3-4%
Sucrose 4-5%
Vitamin C 0.5-0.8%
Mulberry leaf 3-5%
Angelica powder 1-1.5%
Cassia seed powder 0.3-0.5%
Water surplus.
Preferably, the described feed for being conducive to improve beet armyworm reproductive efficiency, including following weight percent at Point:
Corn flour 18%
Yeast powder 7%
Citric acid 0.25%
Sodium benzoate 3.5%
Sucrose 4.5%
Vitamin C 0.65%
Mulberry leaf 4%
Angelica powder 1.25%
Cassia seed powder 0.45%
Water surplus.
Preferably, the described feed for being conducive to improve beet armyworm reproductive efficiency, including following weight percent at Point:
Corn flour 15%
Yeast powder 6%
Citric acid 0.3%
Sodium benzoate 3%
Sucrose 5%
Vitamin C 0.5%
Mulberry leaf 5%
Angelica powder 1%
Cassia seed powder 0.5%
Water surplus.
Preferably, the described feed for being conducive to improve beet armyworm reproductive efficiency, including following weight percent at Point:
Corn flour 15%
Yeast powder 8%
Citric acid 0.2%
Sodium benzoate 4%
Sucrose 4%
Vitamin C 0.8%
Mulberry leaf 3%
Angelica powder 1.5%
Cassia seed powder 0.3%
Water surplus.
Preferably, the yeast powder is feed grade yeast powder.
Feed grade yeast powder is referred exclusively to carbohydrate such as the high concentrated organic waste liquids such as starch, molasses and monosodium glutamate, alcohol For primary raw material, through liquid aerlbic culture saccharomycete, and yeast thallus product obtained after dry is separated from its fermentation, Belong to one of single-cell protein.
The invention has the following advantages: the method for raising beet armyworm of the invention, for beet armyworm growth Different phase sets the different beet armyworm unit area numbers of animals raised, is conducive to its health, safety growth;Using being conducive to mention The feed of high beet armyworm reproductive efficiency is added to angelica powder and cassia seed powder on the basis of traditional beet armyworm feed Two Chinese medicine, combines with mulberry leaf, and beet armyworm immunity can be improved, and improves its larva survival rate, percentage of pupation to reach With the effect of eclosion rate.
Other than objects, features and advantages described above, there are also other objects, features and advantages by the present invention. The present invention is further detailed explanation below.
Specific embodiment
The embodiment of the present invention is described in detail below, but the present invention can be limited and be covered according to claim Multitude of different ways implement.
Embodiment 1
A method of raising beet armyworm follows the steps below:
A, will produce, which has the ovum paper of Beet Armyworm Eggs to be cut into the small scraps of paper with scissors, is placed in sterile petri dish, to ovum blackening Afterwards, it is put into a small amount of feed, abandons ovum paper after larva hatches completely;
B, 1-3 age raises in culture dish always, and larva 280, are put into feed in each culture dish;
C, after larva length to 3 ages, larva 9 in each culture dish are allocated larva into other culture dishes with writing brush, It is put into feed, to long to 4 age ends removing excreta, adds feed, just feed is eaten up when supporting prepupal period, and is allowed to cultivating It pupates in ware.
The feed is the feed for being conducive to improve beet armyworm reproductive efficiency.
The feed for being conducive to improve beet armyworm reproductive efficiency, the ingredient including following weight percent:
Corn flour 18%
Yeast powder 7%
Citric acid 0.25%
Sodium benzoate 3.5%
Sucrose 4.5%
Vitamin C 0.65%
Mulberry leaf 4%
Angelica powder 1.25%
Cassia seed powder 0.45%
Water surplus.
The yeast powder is feed grade yeast powder.
Embodiment 2
A method of raising beet armyworm follows the steps below:
A, will produce, which has the ovum paper of Beet Armyworm Eggs to be cut into the small scraps of paper with scissors, is placed in sterile petri dish, to ovum blackening Afterwards, it is put into a small amount of feed, abandons ovum paper after larva hatches completely;
B, 1-3 age raises in culture dish always, and larva 300, are put into feed in each culture dish;
C, after larva length to 3 ages, larva 8 in each culture dish are allocated larva into other culture dishes with writing brush, It is put into feed, to long to 4 age ends removing excreta, adds feed, just feed is eaten up when supporting prepupal period, and is allowed to cultivating It pupates in ware.
The feed is the feed for being conducive to improve beet armyworm reproductive efficiency.
The feed for being conducive to improve beet armyworm reproductive efficiency, the ingredient including following weight percent:
Corn flour 15%
Yeast powder 6%
Citric acid 0.3%
Sodium benzoate 3%
Sucrose 5%
Vitamin C 0.5%
Mulberry leaf 5%
Angelica powder 1%
Cassia seed powder 0.5%
Water surplus.
The yeast powder is feed grade yeast powder.
Embodiment 3
A method of raising beet armyworm follows the steps below:
A, will produce, which has the ovum paper of Beet Armyworm Eggs to be cut into the small scraps of paper with scissors, is placed in sterile petri dish, to ovum blackening Afterwards, it is put into a small amount of feed, abandons ovum paper after larva hatches completely;
B, 1-3 age raises in culture dish always, and larva 250, are put into feed in each culture dish;
C, after larva length to 3 ages, larva 10 in each culture dish are allocated larva to other culture dishes with writing brush In, it is put into feed, to long to 4 age ends removing excreta, adds feed, just feed is eaten up when supporting prepupal period, and is allowed to It pupates in culture dish.
The feed is the feed for being conducive to improve beet armyworm reproductive efficiency.
The feed for being conducive to improve beet armyworm reproductive efficiency, the ingredient including following weight percent:
Corn flour 15%
Yeast powder 8%
Citric acid 0.2%
Sodium benzoate 4%
Sucrose 4%
Vitamin C 0.8%
Mulberry leaf 3%
Angelica powder 1.5%
Cassia seed powder 0.3%
Water surplus.
The yeast powder is feed grade yeast powder.
Comparative example 1
By in embodiment 1 angelica powder and cassia seed powder remove simultaneously, other conditions are constant.
Comparative example 2
By the angelica powder removal in embodiment 1, other conditions are constant.
Comparative example 3
By the cassia seed powder removal in embodiment 1, other conditions are constant.
Under equal conditions, feeding experiment is carried out to the feed of embodiment 1-3 and comparative example 1-3, is counted as follows According to:
Table 1: beet exigua larvae survival rate, percentage of pupation and eclosion rate data in feeding experiment:
By the above test data it is recognised that being added after angelica powder and cassia seed powder in the present invention, to beet armyworm children Worm survival rate, percentage of pupation and eclosion rate have a promotion by a relatively large margin, the reproductive efficiency general increase of beet armyworm reach 10% with On.
By the above test data it is recognised that being individually added into angelica powder or cassia seed powder, than simultaneously be added angelica powder and The effect of cassia seed powder wants far short of what is expected.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.

Claims (7)

1. a kind of method for raising beet armyworm, which comprises the following steps:
A, will produce, which has the ovum paper of Beet Armyworm Eggs to be cut into the small scraps of paper with scissors, is placed in sterile petri dish, after ovum blackening, puts Enter a small amount of feed, abandons ovum paper after larva hatches completely;
B, 1-3 age raises in culture dish always, and larva 250-300 head, is put into feed in each culture dish;
C, after larva length to 3 ages, larva 8-10 head in each culture dish is allocated larva into other culture dishes with writing brush, It is put into feed, to long to 4 age ends removing excreta, adds feed, just feed is eaten up when supporting prepupal period, and is allowed to cultivating It pupates in ware;The feed is the feed for being conducive to improve beet armyworm reproductive efficiency;Described is conducive to raising beet night The feed of moth reproductive efficiency, the ingredient including following weight percent:
Corn flour 15-25%
Yeast powder 5-8%
Citric acid 0.1-0.3%
Sodium benzoate 2-5%
Sucrose 3-5%
Vitamin C 0.5-1%
Mulberry leaf 3-5%
Angelica powder 1-2%
Cassia seed powder 0.3-0.6%
Water surplus.
2. the method for raising beet armyworm as described in claim 1, which is characterized in that raise the condition of beet armyworm are as follows: temperature 26 ± 1 DEG C of degree, photoperiod illumination 14h/ dark 10h, relative humidity 65-70%R.H..
3. the method for raising beet armyworm as described in claim 1, which is characterized in that described is conducive to raising beet armyworm The feed of reproductive efficiency, the ingredient including following weight percent:
Corn flour 15-20%
Yeast powder 6-8%
Citric acid 0.2-0.3%
Sodium benzoate 3-4%
Sucrose 4-5%
Vitamin C 0.5-0.8%
Mulberry leaf 3-5%
Angelica powder 1-1.5%
Cassia seed powder 0.3-0.5%
Water surplus.
4. the method for raising beet armyworm as described in claim 1, which is characterized in that described is conducive to raising beet armyworm The feed of reproductive efficiency, the ingredient including following weight percent:
Corn flour 18%
Yeast powder 7%
Citric acid 0.25%
Sodium benzoate 3.5%
Sucrose 4.5%
Vitamin C 0.65%
Mulberry leaf 4%
Angelica powder 1.25%
Cassia seed powder 0.45%
Water surplus.
5. the method for raising beet armyworm as described in claim 1, which is characterized in that described is conducive to raising beet armyworm The feed of reproductive efficiency, the ingredient including following weight percent:
Corn flour 15%
Yeast powder 6%
Citric acid 0.3%
Sodium benzoate 3%
Sucrose 5%
Vitamin C 0.5%
Mulberry leaf 5%
Angelica powder 1%
Cassia seed powder 0.5%
Water surplus.
6. the method for raising beet armyworm as described in claim 1, which is characterized in that described is conducive to raising beet armyworm The feed of reproductive efficiency, the ingredient including following weight percent:
Corn flour 15%
Yeast powder 8%
Citric acid 0.2%
Sodium benzoate 4%
Sucrose 4%
Vitamin C 0.8%
Mulberry leaf 3%
Angelica powder 1.5%
Cassia seed powder 0.3%
Water surplus.
7. the method for raising beet armyworm as described in claim 1, which is characterized in that the yeast powder is feed grade yeast Powder.
CN201610691767.2A 2016-08-19 2016-08-19 A method of raising beet armyworm Active CN106259221B (en)

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CN102578432A (en) * 2012-03-27 2012-07-18 董志平 Artificial feed preparation and simplified feeding method for Athelis lepigone larva
RU2013109031A (en) * 2013-02-28 2014-09-10 Елена Александровна Панявина DEVICE FOR KEEPING Worms FROM SPREAD
CN104757333A (en) * 2015-04-23 2015-07-08 中国农业科学院植物保护研究所 Artificial feed for asparagus caterpillar, preparation of artificial feed and feeding method

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CN1347659A (en) * 2001-08-31 2002-05-08 武汉普洛森生物制剂有限公司 Artificial feed for beet noctuids
CN102113471A (en) * 2011-02-22 2011-07-06 山东农业大学 Artificial rearing method of larvae of beet armyworm
CN102578425A (en) * 2012-03-14 2012-07-18 中国农业科学院植物保护研究所 Artificial diet for Lepidopterous young larvae, preparation method for artificial diet and application of artificial diet
CN102578432A (en) * 2012-03-27 2012-07-18 董志平 Artificial feed preparation and simplified feeding method for Athelis lepigone larva
RU2013109031A (en) * 2013-02-28 2014-09-10 Елена Александровна Панявина DEVICE FOR KEEPING Worms FROM SPREAD
CN104757333A (en) * 2015-04-23 2015-07-08 中国农业科学院植物保护研究所 Artificial feed for asparagus caterpillar, preparation of artificial feed and feeding method

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