CN106259221B - A method of raising beet armyworm - Google Patents
A method of raising beet armyworm Download PDFInfo
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- CN106259221B CN106259221B CN201610691767.2A CN201610691767A CN106259221B CN 106259221 B CN106259221 B CN 106259221B CN 201610691767 A CN201610691767 A CN 201610691767A CN 106259221 B CN106259221 B CN 106259221B
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- 241000256247 Spodoptera exigua Species 0.000 title claims abstract description 62
- 238000000034 method Methods 0.000 title claims abstract description 19
- 239000000843 powder Substances 0.000 claims abstract description 67
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 42
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 28
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 26
- 230000001850 reproductive effect Effects 0.000 claims abstract description 22
- 244000037364 Cinnamomum aromaticum Species 0.000 claims abstract description 21
- 235000014489 Cinnamomum aromaticum Nutrition 0.000 claims abstract description 21
- 235000001287 Guettarda speciosa Nutrition 0.000 claims abstract description 21
- 240000000249 Morus alba Species 0.000 claims abstract description 16
- 235000008708 Morus alba Nutrition 0.000 claims abstract description 16
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 14
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 14
- 229930006000 Sucrose Natural products 0.000 claims abstract description 14
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 14
- 240000008042 Zea mays Species 0.000 claims abstract description 14
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 14
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 14
- 235000005822 corn Nutrition 0.000 claims abstract description 14
- 235000013312 flour Nutrition 0.000 claims abstract description 14
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims abstract description 14
- 235000010234 sodium benzoate Nutrition 0.000 claims abstract description 14
- 239000004299 sodium benzoate Substances 0.000 claims abstract description 14
- 239000005720 sucrose Substances 0.000 claims abstract description 14
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 14
- 239000011718 vitamin C Substances 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 241000125175 Angelica Species 0.000 claims description 19
- 102000002322 Egg Proteins Human genes 0.000 claims description 15
- 108010000912 Egg Proteins Proteins 0.000 claims description 15
- 210000004681 ovum Anatomy 0.000 claims description 15
- 239000004615 ingredient Substances 0.000 claims description 8
- 235000013601 eggs Nutrition 0.000 claims description 5
- 210000003608 fece Anatomy 0.000 claims description 5
- 235000016068 Berberis vulgaris Nutrition 0.000 claims description 2
- 241000335053 Beta vulgaris Species 0.000 claims description 2
- 238000005286 illumination Methods 0.000 claims description 2
- 230000032669 eclosion Effects 0.000 abstract description 4
- 230000019617 pupation Effects 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 241001465754 Metazoa Species 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 230000036541 health Effects 0.000 abstract description 2
- 230000036039 immunity Effects 0.000 abstract description 2
- 244000061520 Angelica archangelica Species 0.000 abstract 2
- 241000238631 Hexapoda Species 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 2
- 235000021405 artificial diet Nutrition 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241001124169 Calliphora vomitoria Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 description 1
- 235000013923 monosodium glutamate Nutrition 0.000 description 1
- 239000004223 monosodium glutamate Substances 0.000 description 1
- 239000010815 organic waste Substances 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 108010027322 single cell proteins Proteins 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention provides a kind of methods for raising beet armyworm to set the different beet armyworm unit area numbers of animals raised for the different phase of beet armyworm growth, is conducive to its health, safety growth;Feed includes following main component: corn flour, yeast powder, citric acid, sodium benzoate, sucrose, vitamin C, mulberry leaf, angelica powder, cassia seed powder and water;On the basis of traditional beet armyworm feed, it is added to two Chinese medicine of angelica powder and cassia seed powder, it is combined with mulberry leaf, beet armyworm immunity can be improved, to achieve the effect that improve its larva survival rate, percentage of pupation and eclosion rate, through detecting, the beet armyworm raised using feed of the invention, reproductive efficiency can be improved 10% or more.
Description
Technical field
The present invention relates to insects to raise field, particularly, is related to a kind of method for raising beet armyworm.
Background technique
The lepidoptera pests such as beet armyworm (Spodoptera exigua H ü bner) are a kind of primary pests on vegetables,
With fulminant, binge, the features such as being also easy to produce drug resistance, (department rose cloud etc. 2009;Liu Xiangyangs etc. 2007;Moulton et
Al 2000), cause difficulty of prevention and cure to increase, control cost improves, excessive pesticide residues etc., directly threat food safety, obstruction pair
Outer Trade Development, destroying the ecological balance, (road is triumphant to wait 2005;Huang Yingjia 2013).
It establishes beet armyworm strain, optimizes beet armyworm artificial diet technique, a large amount of normal developments, neat and consistent are provided
Selected insect source is insect Physiology and biochemistry, the element task of the multinomial research such as entomological genetics and insect toxicology.Insect growth week
Phase is short, and population quantity is big, is the best model organism material for carrying out biological study.From the artificial of starting calliphora vomitoria in 1908
After feed formula, the research of insect artificial feeding gradually grows up.Lot of domestic and international scholar carries out insect artificial diet
Research, it is especially most with lepidopterous insects.
Chinese invention patent CN201110386742.9 discloses a kind of feed formula of beet armyworm, and the Feed Material is easy
It obtains, formula of artificial feed for beet armyworm at low cost, can largely breeding beet armyworm, but in order to further increase beet armyworm
Reproductive efficiency, it is necessary to study a kind of new formula of artificial feed for beet armyworm.
Summary of the invention
It is an object of the present invention to study a kind of method for raising beet armyworm.
A method of raising beet armyworm follows the steps below:
A, will produce, which has the ovum paper of Beet Armyworm Eggs to be cut into the small scraps of paper with scissors, is placed in sterile petri dish, to ovum blackening
Afterwards, it is put into a small amount of feed, abandons ovum paper after larva hatches completely;
B, 1-3 age raises in culture dish always, and larva 250-300 head, is put into feed in each culture dish;
C, after larva length to 3 ages, larva 8-10 head in each culture dish is allocated larva to other culture dishes with writing brush
In, it is put into feed, to long to 4 age ends removing excreta, adds feed, just feed is eaten up when supporting prepupal period, and is allowed to
It pupates in culture dish.
Preferably, the condition of the raising beet armyworm are as follows: 26 ± 1 DEG C of temperature, photoperiod illumination 14h/ dark 10h,
Relative humidity is 65-70%R.H..
Preferably, the feed is the feed for being conducive to improve beet armyworm reproductive efficiency.
Preferably, the described feed for being conducive to improve beet armyworm reproductive efficiency, including following weight percent at
Point:
Corn flour 15-25%
Yeast powder 5-8%
Citric acid 0.1-0.3%
Sodium benzoate 2-5%
Sucrose 3-5%
Vitamin C 0.5-1%
Mulberry leaf 3-5%
Angelica powder 1-2%
Cassia seed powder 0.3-0.6%
Water surplus.
Preferably, the described feed for being conducive to improve beet armyworm reproductive efficiency, including following weight percent at
Point:
Corn flour 15-20%
Yeast powder 6-8%
Citric acid 0.2-0.3%
Sodium benzoate 3-4%
Sucrose 4-5%
Vitamin C 0.5-0.8%
Mulberry leaf 3-5%
Angelica powder 1-1.5%
Cassia seed powder 0.3-0.5%
Water surplus.
Preferably, the described feed for being conducive to improve beet armyworm reproductive efficiency, including following weight percent at
Point:
Corn flour 18%
Yeast powder 7%
Citric acid 0.25%
Sodium benzoate 3.5%
Sucrose 4.5%
Vitamin C 0.65%
Mulberry leaf 4%
Angelica powder 1.25%
Cassia seed powder 0.45%
Water surplus.
Preferably, the described feed for being conducive to improve beet armyworm reproductive efficiency, including following weight percent at
Point:
Corn flour 15%
Yeast powder 6%
Citric acid 0.3%
Sodium benzoate 3%
Sucrose 5%
Vitamin C 0.5%
Mulberry leaf 5%
Angelica powder 1%
Cassia seed powder 0.5%
Water surplus.
Preferably, the described feed for being conducive to improve beet armyworm reproductive efficiency, including following weight percent at
Point:
Corn flour 15%
Yeast powder 8%
Citric acid 0.2%
Sodium benzoate 4%
Sucrose 4%
Vitamin C 0.8%
Mulberry leaf 3%
Angelica powder 1.5%
Cassia seed powder 0.3%
Water surplus.
Preferably, the yeast powder is feed grade yeast powder.
Feed grade yeast powder is referred exclusively to carbohydrate such as the high concentrated organic waste liquids such as starch, molasses and monosodium glutamate, alcohol
For primary raw material, through liquid aerlbic culture saccharomycete, and yeast thallus product obtained after dry is separated from its fermentation,
Belong to one of single-cell protein.
The invention has the following advantages: the method for raising beet armyworm of the invention, for beet armyworm growth
Different phase sets the different beet armyworm unit area numbers of animals raised, is conducive to its health, safety growth;Using being conducive to mention
The feed of high beet armyworm reproductive efficiency is added to angelica powder and cassia seed powder on the basis of traditional beet armyworm feed
Two Chinese medicine, combines with mulberry leaf, and beet armyworm immunity can be improved, and improves its larva survival rate, percentage of pupation to reach
With the effect of eclosion rate.
Other than objects, features and advantages described above, there are also other objects, features and advantages by the present invention.
The present invention is further detailed explanation below.
Specific embodiment
The embodiment of the present invention is described in detail below, but the present invention can be limited and be covered according to claim
Multitude of different ways implement.
Embodiment 1
A method of raising beet armyworm follows the steps below:
A, will produce, which has the ovum paper of Beet Armyworm Eggs to be cut into the small scraps of paper with scissors, is placed in sterile petri dish, to ovum blackening
Afterwards, it is put into a small amount of feed, abandons ovum paper after larva hatches completely;
B, 1-3 age raises in culture dish always, and larva 280, are put into feed in each culture dish;
C, after larva length to 3 ages, larva 9 in each culture dish are allocated larva into other culture dishes with writing brush,
It is put into feed, to long to 4 age ends removing excreta, adds feed, just feed is eaten up when supporting prepupal period, and is allowed to cultivating
It pupates in ware.
The feed is the feed for being conducive to improve beet armyworm reproductive efficiency.
The feed for being conducive to improve beet armyworm reproductive efficiency, the ingredient including following weight percent:
Corn flour 18%
Yeast powder 7%
Citric acid 0.25%
Sodium benzoate 3.5%
Sucrose 4.5%
Vitamin C 0.65%
Mulberry leaf 4%
Angelica powder 1.25%
Cassia seed powder 0.45%
Water surplus.
The yeast powder is feed grade yeast powder.
Embodiment 2
A method of raising beet armyworm follows the steps below:
A, will produce, which has the ovum paper of Beet Armyworm Eggs to be cut into the small scraps of paper with scissors, is placed in sterile petri dish, to ovum blackening
Afterwards, it is put into a small amount of feed, abandons ovum paper after larva hatches completely;
B, 1-3 age raises in culture dish always, and larva 300, are put into feed in each culture dish;
C, after larva length to 3 ages, larva 8 in each culture dish are allocated larva into other culture dishes with writing brush,
It is put into feed, to long to 4 age ends removing excreta, adds feed, just feed is eaten up when supporting prepupal period, and is allowed to cultivating
It pupates in ware.
The feed is the feed for being conducive to improve beet armyworm reproductive efficiency.
The feed for being conducive to improve beet armyworm reproductive efficiency, the ingredient including following weight percent:
Corn flour 15%
Yeast powder 6%
Citric acid 0.3%
Sodium benzoate 3%
Sucrose 5%
Vitamin C 0.5%
Mulberry leaf 5%
Angelica powder 1%
Cassia seed powder 0.5%
Water surplus.
The yeast powder is feed grade yeast powder.
Embodiment 3
A method of raising beet armyworm follows the steps below:
A, will produce, which has the ovum paper of Beet Armyworm Eggs to be cut into the small scraps of paper with scissors, is placed in sterile petri dish, to ovum blackening
Afterwards, it is put into a small amount of feed, abandons ovum paper after larva hatches completely;
B, 1-3 age raises in culture dish always, and larva 250, are put into feed in each culture dish;
C, after larva length to 3 ages, larva 10 in each culture dish are allocated larva to other culture dishes with writing brush
In, it is put into feed, to long to 4 age ends removing excreta, adds feed, just feed is eaten up when supporting prepupal period, and is allowed to
It pupates in culture dish.
The feed is the feed for being conducive to improve beet armyworm reproductive efficiency.
The feed for being conducive to improve beet armyworm reproductive efficiency, the ingredient including following weight percent:
Corn flour 15%
Yeast powder 8%
Citric acid 0.2%
Sodium benzoate 4%
Sucrose 4%
Vitamin C 0.8%
Mulberry leaf 3%
Angelica powder 1.5%
Cassia seed powder 0.3%
Water surplus.
The yeast powder is feed grade yeast powder.
Comparative example 1
By in embodiment 1 angelica powder and cassia seed powder remove simultaneously, other conditions are constant.
Comparative example 2
By the angelica powder removal in embodiment 1, other conditions are constant.
Comparative example 3
By the cassia seed powder removal in embodiment 1, other conditions are constant.
Under equal conditions, feeding experiment is carried out to the feed of embodiment 1-3 and comparative example 1-3, is counted as follows
According to:
Table 1: beet exigua larvae survival rate, percentage of pupation and eclosion rate data in feeding experiment:
By the above test data it is recognised that being added after angelica powder and cassia seed powder in the present invention, to beet armyworm children
Worm survival rate, percentage of pupation and eclosion rate have a promotion by a relatively large margin, the reproductive efficiency general increase of beet armyworm reach 10% with
On.
By the above test data it is recognised that being individually added into angelica powder or cassia seed powder, than simultaneously be added angelica powder and
The effect of cassia seed powder wants far short of what is expected.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (7)
1. a kind of method for raising beet armyworm, which comprises the following steps:
A, will produce, which has the ovum paper of Beet Armyworm Eggs to be cut into the small scraps of paper with scissors, is placed in sterile petri dish, after ovum blackening, puts
Enter a small amount of feed, abandons ovum paper after larva hatches completely;
B, 1-3 age raises in culture dish always, and larva 250-300 head, is put into feed in each culture dish;
C, after larva length to 3 ages, larva 8-10 head in each culture dish is allocated larva into other culture dishes with writing brush,
It is put into feed, to long to 4 age ends removing excreta, adds feed, just feed is eaten up when supporting prepupal period, and is allowed to cultivating
It pupates in ware;The feed is the feed for being conducive to improve beet armyworm reproductive efficiency;Described is conducive to raising beet night
The feed of moth reproductive efficiency, the ingredient including following weight percent:
Corn flour 15-25%
Yeast powder 5-8%
Citric acid 0.1-0.3%
Sodium benzoate 2-5%
Sucrose 3-5%
Vitamin C 0.5-1%
Mulberry leaf 3-5%
Angelica powder 1-2%
Cassia seed powder 0.3-0.6%
Water surplus.
2. the method for raising beet armyworm as described in claim 1, which is characterized in that raise the condition of beet armyworm are as follows: temperature
26 ± 1 DEG C of degree, photoperiod illumination 14h/ dark 10h, relative humidity 65-70%R.H..
3. the method for raising beet armyworm as described in claim 1, which is characterized in that described is conducive to raising beet armyworm
The feed of reproductive efficiency, the ingredient including following weight percent:
Corn flour 15-20%
Yeast powder 6-8%
Citric acid 0.2-0.3%
Sodium benzoate 3-4%
Sucrose 4-5%
Vitamin C 0.5-0.8%
Mulberry leaf 3-5%
Angelica powder 1-1.5%
Cassia seed powder 0.3-0.5%
Water surplus.
4. the method for raising beet armyworm as described in claim 1, which is characterized in that described is conducive to raising beet armyworm
The feed of reproductive efficiency, the ingredient including following weight percent:
Corn flour 18%
Yeast powder 7%
Citric acid 0.25%
Sodium benzoate 3.5%
Sucrose 4.5%
Vitamin C 0.65%
Mulberry leaf 4%
Angelica powder 1.25%
Cassia seed powder 0.45%
Water surplus.
5. the method for raising beet armyworm as described in claim 1, which is characterized in that described is conducive to raising beet armyworm
The feed of reproductive efficiency, the ingredient including following weight percent:
Corn flour 15%
Yeast powder 6%
Citric acid 0.3%
Sodium benzoate 3%
Sucrose 5%
Vitamin C 0.5%
Mulberry leaf 5%
Angelica powder 1%
Cassia seed powder 0.5%
Water surplus.
6. the method for raising beet armyworm as described in claim 1, which is characterized in that described is conducive to raising beet armyworm
The feed of reproductive efficiency, the ingredient including following weight percent:
Corn flour 15%
Yeast powder 8%
Citric acid 0.2%
Sodium benzoate 4%
Sucrose 4%
Vitamin C 0.8%
Mulberry leaf 3%
Angelica powder 1.5%
Cassia seed powder 0.3%
Water surplus.
7. the method for raising beet armyworm as described in claim 1, which is characterized in that the yeast powder is feed grade yeast
Powder.
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