CN103782969A - Method for breeding trichogramma pintoi indoors and corcyra cephalonica raising fodder - Google Patents
Method for breeding trichogramma pintoi indoors and corcyra cephalonica raising fodder Download PDFInfo
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- CN103782969A CN103782969A CN201410074195.4A CN201410074195A CN103782969A CN 103782969 A CN103782969 A CN 103782969A CN 201410074195 A CN201410074195 A CN 201410074195A CN 103782969 A CN103782969 A CN 103782969A
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- 238000009395 breeding Methods 0.000 title claims abstract description 56
- 230000001488 breeding effect Effects 0.000 title claims abstract description 56
- 241001248473 Trichogramma pintoi Species 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 29
- 241000993412 Corcyra cephalonica Species 0.000 title abstract 6
- 235000013601 eggs Nutrition 0.000 claims abstract description 79
- 235000013312 flour Nutrition 0.000 claims abstract description 69
- 240000008042 Zea mays Species 0.000 claims abstract description 32
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 32
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 32
- 235000005822 corn Nutrition 0.000 claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 235000019733 Fish meal Nutrition 0.000 claims abstract description 17
- 239000004467 fishmeal Substances 0.000 claims abstract description 17
- 238000011081 inoculation Methods 0.000 claims abstract description 16
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 12
- 241000607479 Yersinia pestis Species 0.000 claims abstract description 8
- 239000000463 material Substances 0.000 claims abstract description 7
- 238000009835 boiling Methods 0.000 claims abstract description 4
- 241000256844 Apis mellifera Species 0.000 claims description 105
- 241000753145 Sitotroga cerealella Species 0.000 claims description 91
- 102000002322 Egg Proteins Human genes 0.000 claims description 47
- 108010000912 Egg Proteins Proteins 0.000 claims description 47
- 210000004681 ovum Anatomy 0.000 claims description 47
- 241000238631 Hexapoda Species 0.000 claims description 22
- 239000000203 mixture Substances 0.000 claims description 22
- 238000002360 preparation method Methods 0.000 claims description 17
- 241000256618 Trichogramma Species 0.000 claims description 15
- 239000002994 raw material Substances 0.000 claims description 12
- 241000326498 Ophiorrhabda cellifera Species 0.000 claims description 10
- 240000002044 Rhizophora apiculata Species 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 238000012546 transfer Methods 0.000 claims description 10
- 230000032669 eclosion Effects 0.000 claims description 6
- 230000024241 parasitism Effects 0.000 claims description 6
- 229920000742 Cotton Polymers 0.000 claims description 5
- 241000382353 Pupa Species 0.000 claims description 5
- 230000036528 appetite Effects 0.000 claims description 5
- 235000019789 appetite Nutrition 0.000 claims description 5
- 229910002114 biscuit porcelain Inorganic materials 0.000 claims description 5
- 239000002775 capsule Substances 0.000 claims description 5
- 235000013339 cereals Nutrition 0.000 claims description 5
- 230000002950 deficient Effects 0.000 claims description 5
- 230000003203 everyday effect Effects 0.000 claims description 5
- 239000004744 fabric Substances 0.000 claims description 5
- 230000012447 hatching Effects 0.000 claims description 5
- 210000001161 mammalian embryo Anatomy 0.000 claims description 5
- 230000000474 nursing effect Effects 0.000 claims description 5
- 238000004321 preservation Methods 0.000 claims description 5
- 230000019617 pupation Effects 0.000 claims description 5
- 238000010408 sweeping Methods 0.000 claims description 5
- 230000008901 benefit Effects 0.000 abstract description 3
- 230000017448 oviposition Effects 0.000 abstract description 2
- 235000015099 wheat brans Nutrition 0.000 abstract 2
- 239000000843 powder Substances 0.000 abstract 1
- 230000003071 parasitic effect Effects 0.000 description 8
- 238000011160 research Methods 0.000 description 5
- 241001441330 Grapholita molesta Species 0.000 description 3
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- 241000289763 Dasygaster padockina Species 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 241001480238 Steinernema Species 0.000 description 1
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- 238000011031 large-scale manufacturing process Methods 0.000 description 1
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Abstract
The invention provides a method for breeding trichogramma pintoi indoors and corcyra cephalonica raising fodder. The corcyra cephalonica raising fodder comprises fodder A and fodder B, wherein the fodder A is composed of, by weight, 50 parts of rough wheat bran, 40 parts of corn flour, 10 parts of flour and 5 parts of water; the fodder B is composed of, by weight, 50 parts of rough wheat bran, 40 parts of corn flour, 5 parts of flour, 3 parts of fish meal, 2 parts of yeast powder and 5 parts of water. The method uses corcyra cephalonica eggs as intermediate hosts, formula fodder of corcyra cephalonica larvae at different periods is prepared by adopting a plurality of kinds of fodder, the corcyra cephalonica larvae are bred by the formula fodder so that emergence tidiness of adults can be achieved, the egg laying amount is high, and the mode that a boiling tube is connected in egg exposure is adopted for large-amount inoculation and breeding of the trichogramma pintoi. The method does not need a large device, breeding materials are simple and easy to obtain, operation is simple, practicality is high, environment is protected, pollution is avoided, the produced trichogramma pintoi can be used for biological control of a plurality of kinds of agriculture and forestry pests, and good economical, ecological and social benefits are obtained.
Description
Technical field
The invention belongs to agriculture and forestry injurious insect biological prevention field, relate to a kind of method and rice moth culturing feed of indoor propagation Trichogramma pintoi.
Background technology
Oophagous trichogrammae (
trichogrammaspp.) discovery existing more than 100 year apart from the present, ovum that can parasitic multiple lepidopterous insects, is a class ovum parasite most widely used in whole world biological control of insect pests.China has just carried out application study to oophagous trichogrammae since nineteen thirties, from 1934, Zhu Ruzuo, Hu Yongxi stop to the seventies from having found oophagous trichogrammae in pine tag worm's ovum at the Tang Xixian of Zhejiang Province, and Pu host, large scale artificial breeding and the field bulk storage effects etc. to oophagous trichogrammae such as dragon of stinging have been carried out systematic research.After the eighties, China's oophagous trichogrammae research and application technology have obtained further reinforcement, and oophagous trichogrammae classification, behavior, physiological ecological and artificial host egg's application aspect has been carried out a large amount of work.Along with improving constantly of the development of sustainable agriculture and the requirement of pollution-free green food industry, this biological prevention that is master with natural enemy is more and more accepted by vast farmers.Trichogramma pintoi is the natural enemy that a class has value very much, can be used for the multiple agriculture and forestry injurious insect biological controls such as cotton bollworm, beet webworm, cutworm, steinernema.The institute of agricultural sciences of Yuncheng Prefecture of Shanxi Province to Trichogramma pintoi biological characteristics seek peace parasitic cotton bollworm condition survey observe; Wang Fucheng, a comb instrument produces the research of the Trichogramma pintoi of both sexes filial generation to parthenogenesis; Ma Deying etc. prevent and treat Cotton Boll Worm Populations From Xinjiang Uygur Autonomous Region Preliminary Report on Experiment to Trichogramma pintoi, and have carried out the impact research of term refrigeration on different lines Trichogramma pintoi; Tian Xiaoxia etc. find the parasitic beet webworm ovum of Trichogramma pintoi first in China; Shen Jian, is permitted to build up the Army to wait and has been carried out the impact of ultraviolet processing oriental fruit moth ovum on Trichogramma pintoi parasitism and emergence, impact and the Trichogramma pintoi parasitization research to oriental fruit moth ovum of high temperature stress on Trichogramma pintoi parasitic functional response.And for the artificial propagation techniques of Trichogramma pintoi except coroner all auspicious grade introduced and a kind of method of utilizing oriental fruit moth ovum indoor propagation Trichogramma pintoi, do not seen other report, and the method is difficult to large-scale production and is applied to biological control of insect pests.
Summary of the invention
The object of this invention is to provide a kind of efficiently in the indoor method of utilizing rice moth egg to carry out amount reproduction Trichogramma pintoi; Another object of the present invention is to provide a kind of rice moth feed efficiently.
In order to achieve the above object, the present invention adopts following technical scheme:
A rice moth culturing feed for indoor propagation Trichogramma pintoi, rice moth culturing feed divides A feed and B feed, and its preparation raw material composition and parts by weight are respectively:
Raw material composition and the parts by weight of A feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 10 parts, flour and water;
Raw material composition and the parts by weight of B feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 5 parts, flour, 3 parts of fish meal, 2 parts of dusty yeasts and water.
Described A feed and the preparation method of B feed be respectively:
(1) preparation of A feed: first the brown shorts of A feedstuff composition, corn flour, flour are put into disinfection cabinet and heat, temperature is sterilized at keeping 60-90 ℃ 4-5 hour, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour and flour fully to mix thoroughly again and obtain A feed;
(2) preparation of B feed: first the brown shorts of B feedstuff composition, corn flour, flour, fish meal are put into disinfection cabinet and heat, temperature is sterilized at keeping 60-90 ℃ 4-5 hour, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour, flour, fish meal fully to mix thoroughly again and obtain B feed together with dusty yeast.
A kind of method of indoor propagation Trichogramma pintoi, in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, to be returned indoor collection Trichogramma pintoi as honeybee kind provenance by the ovum collection of Trichogramma, breed in a large number as vector using rice moth egg indoor, propagation method comprises the following steps:
(1) breeding of rice moth: when rice moth inoculation, at the thick A feed of plastic crate middle berth one deck 1.1-1.2 cm, on feed, evenly spill rice moth kind ovum, free-and-easy one deck just covers the flour bisque of ovum grain again, is placed in rice moth and raises indoor feeding, and the larva after hatching starts feeding after eating out thickly dotted aperture, within first 15 days, throw in A feed in inoculation, throw in afterwards B feed, within every 3 days, throw a defective material, input amount is that the larva appetite of 3 days is until larvae pupation; Pupa is collected adult after sprouting wings and is placed in the cage of laying eggs and lays eggs, and collects rice moth egg every day, and part is directly used in the breeding of rice moth, and remainder kills and performs mark after embryo and put into the refrigerator of 3-5 ℃ and preserve with ultraviolet light, is made into rice moth egg card before numerous honeybee, for subsequent use;
(2) field bee kind: in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, gather by the ovum of Trichogramma, return indoor bottle and collect Trichogramma pintoi as honeybee kind provenance, in the bottle of collecting honeybee kind, access rice moth egg card, parasitism is bred into oophagous trichogrammae breeding kind of honeybee card, for subsequent use;
(3) breeding of oophagous trichogrammae: 1 oophagous trichogrammae breeding kind of honeybee card is put into breeding bee tube numerous honeybee is indoor, clog the mouth of pipe with cotton plug, on honeybee card, snap into and in breeding bee tube, allow Trichogramma at 8 rice moth eggs of transfer room access when the bee eclosion of 60-70 %, put into the filter paper bar nursing of sweeping 10 % hydromels simultaneously, when access ovum card, the other end of the mouth of pipe is to light, and anti-kind of honeybee escapes from, after having inoculated, all breeding bee tubes that accessed ovum card are being palletized and covering shading with black cloth, then move into that numerous honeybee is indoor to lay eggs, after 8-14 hour, in transfer room, change to 8 new rice moth egg cards with same inoculation method, every breeding kind of honeybee card can connect 16 rice moth egg cards, postvaccinal rice moth egg card is standing and is being put in capsule and moves into that numerous honeybee is indoor continues to breed 4-5 days, then the refrigerator of putting into 3-5 ℃ carries out short-term preservation, during with honeybee, shift to an earlier date 4-7 days and take out and be placed in the indoor cultivation of numerous honeybee from refrigerator, the available expansion of honeybee card after cultivation is numerous or for biological control of insect pests.
Described rice moth receptacle control indoor temperature is 25-28 ℃, and relative moisture is 60-85 %.
It is 25-28 ℃ that indoor temperature is controlled in described numerous honeybee chamber, and relative moisture is 70-85 %.
Described breeding bee tube is the Boiling tube of diameter 30 mm × length 20 cm.
Described rice moth egg card is that the sticky rice moth egg of the strip label card of 2 cm × 4 cm is made.
Advantage of the present invention and beneficial effect have:
(1) Trichogramma pintoi that the present invention breeds is the extensive natural enemy insect of a class host, can be used in the biological control of multiple agriculture and forestry injurious insect.
(2) in rice moth of the present invention breeding, in larvae feeds, added fish meal, dusty yeast makes rice moth adult eclosion more neat, egg laying amount is higher.
(3) make the parasitic rate of oophagous trichogrammae higher with the numerous honeybee of Boiling tube, mean parasitized rate reaches more than 90%.
(4) the present invention is without large-scale instrument, and propagating materials used is easily obtained, simple to operate, practical.
(5) the present invention is environmentally friendly, pollution-free, is suitable for application and promotes, and has good social benefit.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described.
embodiment 1:
One, the rice moth culturing feed of breeding Trichogramma pintoi:
Raw material composition and the parts by weight of A feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 10 parts, flour and water;
Raw material composition and the parts by weight of B feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 5 parts, flour, 3 parts of fish meal, 2 parts of dusty yeasts and water.
Described A feed and the preparation method of B feed be respectively:
(1) preparation of A feed: first the brown shorts of A feedstuff composition, corn flour, flour are put into disinfection cabinet and heat, temperature is sterilized 5 hours at keeping 60 ℃, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour and flour fully to mix thoroughly again and obtain A feed;
(2) preparation of B feed: first the brown shorts of B feedstuff composition, corn flour, flour, fish meal are put into disinfection cabinet and heat, temperature is sterilized 5 hours at keeping 60 ℃, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour, flour, fish meal fully to mix thoroughly again and obtain B feed together with dusty yeast.
Two, the method for indoor propagation Trichogramma pintoi:
In mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, using being returned indoor collection Trichogramma pintoi as honeybee kind provenance by the ovum collection of Trichogramma, to breed in a large number as vector using rice moth egg indoor, propagation method comprises the following steps:
(1) breeding of rice moth: when rice moth inoculation, at the thick A feed of plastic crate middle berth one deck 1.1cm, on feed, evenly spill rice moth kind ovum, free-and-easy one deck just covers the flour bisque of ovum grain again, be placed in rice moth and raise indoor feeding, controlling indoor temperature is 25 ℃, relative moisture is 70 %, larva after hatching starts feeding after eating out thickly dotted aperture, within first 15 days, throw in A feed in inoculation, throw in afterwards B feed, within every 3 days, throw a defective material, input amount is that the larva appetite of 3 days is until larvae pupation; The rear collection of pupa emergence adult is placed in the cage of laying eggs and lays eggs, collect rice moth egg every day, part is directly used in the breeding of rice moth, remainder kills and performs mark after embryo and put into the refrigerator of 3 ℃ and preserve with ultraviolet light, the sticky rice moth egg of strip label card with 2 cm × 4 cm before numerous honeybee is made into rice moth egg card, for subsequent use;
(3) field bee kind: in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, gather by the ovum of Trichogramma, return indoor bottle and collect Trichogramma pintoi as honeybee kind provenance, in the bottle of collecting honeybee kind, access rice moth egg card, parasitism is bred into oophagous trichogrammae breeding kind of honeybee card, for subsequent use;
(4) breeding of oophagous trichogrammae: at the indoor breeding bee tube of 1 oophagous trichogrammae breeding kind of honeybee card being put into diameter 30mm × long 20cm of numerous honeybee, clog the mouth of pipe with cotton plug, on honeybee card, snap into and in breeding bee tube, allow Trichogramma at 8 rice moth eggs of transfer room access when 60% bee eclosion, put into the filter paper bar nursing of sweeping 10 % hydromels simultaneously, when access ovum card, the other end of the mouth of pipe is to light, and anti-kind of honeybee escapes from, after having inoculated, all breeding bee tubes that accessed ovum card are being palletized and covering shading with black cloth, then move into that numerous honeybee is indoor to lay eggs, controlling indoor temperature is 25 ℃, relative moisture is 70 %, after 8 hours, in transfer room, change to 8 new rice moth egg cards with same inoculation method, every breeding kind of honeybee card can connect 16 rice moth egg cards, postvaccinal rice moth egg card is standing and is being put in capsule and moves into that numerous honeybee is indoor to be continued to breed 4 days, mean parasitized rate reaches 90.6%, then the refrigerator of parasitic rice moth egg card being put into 3 ℃ carries out short-term preservation, during with honeybee, within 7 days, take out and be placed in the indoor cultivation of numerous honeybee from refrigerator in advance, the available expansion of honeybee card after cultivation is numerous or for biological control of insect pests.
embodiment 2:
One, the rice moth culturing feed of breeding Trichogramma pintoi:
Raw material composition and the parts by weight of A feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 10 parts, flour and water;
Raw material composition and the parts by weight of B feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 5 parts, flour, 3 parts of fish meal, 2 parts of dusty yeasts and water.
Described A feed and the preparation method of B feed be respectively:
(1) preparation of A feed: first the brown shorts of A feedstuff composition, corn flour, flour are put into disinfection cabinet and heat, temperature is sterilized 5 hours at keeping 70 ℃, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour and flour fully to mix thoroughly again and obtain A feed;
(2) preparation of B feed: first the brown shorts of B feedstuff composition, corn flour, flour, fish meal are put into disinfection cabinet and heat, temperature is sterilized 5 hours at keeping 70 ℃, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour, flour, fish meal fully to mix thoroughly again and obtain B feed together with dusty yeast.
Two, the method for indoor propagation Trichogramma pintoi:
In mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, using being returned indoor collection Trichogramma pintoi as honeybee kind provenance by the ovum collection of Trichogramma, to breed in a large number as vector using rice moth egg indoor, propagation method comprises the following steps:
(1) breeding of rice moth: when rice moth inoculation, at the thick A feed of plastic crate middle berth one deck 1.1cm, on feed, evenly spill rice moth kind ovum, free-and-easy one deck just covers the flour bisque of ovum grain again, be placed in rice moth and raise indoor feeding, controlling indoor temperature is 26 ℃, relative moisture is 75 %, larva after hatching starts feeding after eating out thickly dotted aperture, within first 15 days, throw in A feed in inoculation, throw in afterwards B feed, within every 3 days, throw a defective material, input amount is that the larva appetite of 3 days is until larvae pupation; The rear collection of pupa emergence adult is placed in the cage of laying eggs and lays eggs, collect rice moth egg every day, part is directly used in the breeding of rice moth, remainder kills and performs mark after embryo and put into the refrigerator of 4 ℃ and preserve with ultraviolet light, the sticky rice moth egg of strip label card with 2 cm × 4 cm before numerous honeybee is made into rice moth egg card, for subsequent use;
(3) field bee kind: in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, gather by the ovum of Trichogramma, return indoor bottle and collect Trichogramma pintoi as honeybee kind provenance, in the bottle of collecting honeybee kind, access rice moth egg card, parasitism is bred into oophagous trichogrammae breeding kind of honeybee card, for subsequent use;
(4) breeding of oophagous trichogrammae: at the indoor breeding bee tube of 1 oophagous trichogrammae breeding kind of honeybee card being put into diameter 30mm × long 20cm of numerous honeybee, clog the mouth of pipe with cotton plug, on honeybee card, snap into and in breeding bee tube, allow Trichogramma at 8 rice moth eggs of transfer room access when the bee eclosion of 65 %, put into the filter paper bar nursing of sweeping 10 % hydromels simultaneously, when access ovum card, the other end of the mouth of pipe is to light, and anti-kind of honeybee escapes from, after having inoculated, all breeding bee tubes that accessed ovum card are being palletized and covering shading with black cloth, then move into that numerous honeybee is indoor to lay eggs, controlling indoor temperature is 26 ℃, relative moisture is 75 %, after 10 hours, in transfer room, change to 8 new rice moth egg cards with same inoculation method, every breeding kind of honeybee card can connect 16 rice moth egg cards, postvaccinal rice moth egg card is standing and is being put in capsule and moves into that numerous honeybee is indoor to be continued to breed 5 days, mean parasitized rate reaches 92.1%, then the refrigerator of parasitic rice moth egg card being put into 4 ℃ carries out short-term preservation, during with honeybee, within 5 days, take out and be placed in the indoor cultivation of numerous honeybee from refrigerator in advance, the available expansion of honeybee card after cultivation is numerous or for biological control of insect pests.
embodiment 3:
One, the rice moth culturing feed of breeding Trichogramma pintoi:
Raw material composition and the parts by weight of A feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 10 parts, flour and water;
Raw material composition and the parts by weight of B feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 5 parts, flour, 3 parts of fish meal, 2 parts of dusty yeasts and water.
Described A feed and the preparation method of B feed be respectively:
(1) preparation of A feed: first the brown shorts of A feedstuff composition, corn flour, flour are put into disinfection cabinet and heat, temperature is sterilized 4 hours at keeping 90 ℃, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour and flour fully to mix thoroughly again and obtain A feed;
(2) preparation of B feed: first the brown shorts of B feedstuff composition, corn flour, flour, fish meal are put into disinfection cabinet and heat, temperature is sterilized 4 hours at keeping 90 ℃, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour, flour, fish meal fully to mix thoroughly again and obtain B feed together with dusty yeast.
Two, the method for indoor propagation Trichogramma pintoi:
In mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, using being returned indoor collection Trichogramma pintoi as honeybee kind provenance by the ovum collection of Trichogramma, to breed in a large number as vector using rice moth egg indoor, propagation method comprises the following steps:
(1) breeding of rice moth: when rice moth inoculation, at the thick A feed of plastic crate middle berth one deck 1.2 cm, on feed, evenly spill rice moth kind ovum, free-and-easy one deck just covers the flour bisque of ovum grain again, be placed in rice moth and raise indoor feeding, controlling indoor temperature is 28 ℃, relative moisture is 80 %, larva after hatching starts feeding after eating out thickly dotted aperture, within first 15 days, throw in A feed in inoculation, throw in afterwards B feed, within every 3 days, throw a defective material, input amount is that the larva appetite of 3 days is until larvae pupation; The rear collection of pupa emergence adult is placed in the cage of laying eggs and lays eggs, collect rice moth egg every day, part is directly used in the breeding of rice moth, remainder kills and performs mark after embryo and put into the refrigerator of 5 ℃ and preserve with ultraviolet light, the sticky rice moth egg of strip label card with 2 cm × 4 cm before numerous honeybee is made into rice moth egg card, for subsequent use;
(3) field bee kind: in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, gather by the ovum of Trichogramma, return indoor bottle and collect Trichogramma pintoi as honeybee kind provenance, in the bottle of collecting honeybee kind, access rice moth egg card, parasitism is bred into oophagous trichogrammae breeding kind of honeybee card, for subsequent use;
(4) breeding of oophagous trichogrammae: at the indoor breeding bee tube of 1 oophagous trichogrammae breeding kind of honeybee card being put into diameter 30mm × long 20cm of numerous honeybee, clog the mouth of pipe with cotton plug, on honeybee card, snap into and in breeding bee tube, allow Trichogramma at 8 rice moth eggs of transfer room access when 70% bee eclosion, put into the filter paper bar nursing of sweeping 10 % hydromels simultaneously, when access ovum card, the other end of the mouth of pipe is to light, and anti-kind of honeybee escapes from, after having inoculated, all breeding bee tubes that accessed ovum card are being palletized and covering shading with black cloth, then move into that numerous honeybee is indoor to lay eggs, controlling indoor temperature is 28 ℃, relative moisture is 80 %, after 12 hours, in transfer room, change to 8 new rice moth egg cards with same inoculation method, every breeding kind of honeybee card can connect 16 rice moth egg cards, postvaccinal rice moth egg card is standing and is being put in capsule and moves into that numerous honeybee is indoor to be continued to breed 4 days, mean parasitized rate reaches 91.3%, then the refrigerator of parasitic rice moth egg card being put into 5 ℃ carries out short-term preservation, during with honeybee, within 6 days, take out and be placed in the indoor cultivation of numerous honeybee from refrigerator in advance, the available expansion of honeybee card after cultivation is numerous or for biological control of insect pests.
Claims (7)
1. a rice moth culturing feed for indoor propagation Trichogramma pintoi, is characterized in that: rice moth culturing feed divides A feed and B feed, and its preparation raw material composition and parts by weight are respectively:
Raw material composition and the parts by weight of A feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 10 parts, flour and water;
Raw material composition and the parts by weight of B feed:
5 parts, 50 parts of brown shorts, 40 parts of corn flour, 5 parts, flour, 3 parts of fish meal, 2 parts of dusty yeasts and water.
2. the rice moth culturing feed of a kind of indoor propagation Trichogramma pintoi according to claim 1, is characterized in that: described A feed and the preparation method of B feed respectively:
(1) preparation of A feed: first the brown shorts of A feedstuff composition, corn flour, flour are put into disinfection cabinet and heat, temperature is sterilized at keeping 60-90 ℃ 4-5 hour, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour and flour fully to mix thoroughly again and obtain A feed;
(2) preparation of feed: first the brown shorts of B feedstuff composition, corn flour, flour, fish meal are put into disinfection cabinet and heat, temperature is sterilized at keeping 60-90 ℃ 4-5 hour, kill germ, insect, evil mite and ovum thereof in feed, again brown shorts and water are fully mixed thoroughly, then added corn flour, flour, fish meal fully to mix thoroughly again and obtain B feed together with dusty yeast.
3. the method for an indoor propagation Trichogramma pintoi, it is characterized in that: in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, to be returned indoor collection Trichogramma pintoi as honeybee kind provenance by the ovum collection of Trichogramma, breed in a large number as vector using rice moth egg indoor, propagation method comprises the following steps:
(1) breeding of rice moth: when rice moth inoculation, at the thick A feed of plastic crate middle berth one deck 1.1-1.2cm, on feed, evenly spill rice moth kind ovum, free-and-easy one deck just covers the flour bisque of ovum grain again, is placed in rice moth and raises indoor feeding, and the larva after hatching starts feeding after eating out thickly dotted aperture, within first 15 days, throw in A feed in inoculation, throw in afterwards B feed, within every 3 days, throw a defective material, input amount is that the larva appetite of 3 days is until larvae pupation; Pupa is collected adult after sprouting wings and is placed in the cage of laying eggs and lays eggs, and collects rice moth egg every day, and part is directly used in the breeding of rice moth, and remainder kills and performs mark after embryo and put into the refrigerator of 3-5 ℃ and preserve with ultraviolet light, is made into rice moth egg card before numerous honeybee, for subsequent use;
(2) field bee kind: in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, gather by the ovum of Trichogramma, return indoor bottle and collect Trichogramma pintoi as honeybee kind provenance, in the bottle of collecting honeybee kind, access rice moth egg card, parasitism is bred into oophagous trichogrammae breeding kind of honeybee card, for subsequent use;
(3) breeding of oophagous trichogrammae: 1 oophagous trichogrammae breeding kind of honeybee card is put into breeding bee tube numerous honeybee is indoor, clog the mouth of pipe with cotton plug, on honeybee card, snap into and in breeding bee tube, allow Trichogramma at 8 rice moth eggs of transfer room access when the bee eclosion of 60-70 %, put into the filter paper bar nursing of sweeping 10 % hydromels simultaneously, when access ovum card, the other end of the mouth of pipe is to light, and anti-kind of honeybee escapes from, after having inoculated, all breeding bee tubes that accessed ovum card are being palletized and covering shading with black cloth, then move into that numerous honeybee is indoor to lay eggs, after 8-14 hour, in transfer room, change to 8 new rice moth egg cards with same inoculation method, every breeding kind of honeybee card can connect 16 rice moth egg cards, postvaccinal rice moth egg card is standing and is being put in capsule and moves into that numerous honeybee is indoor continues to breed 4-5 days, then the refrigerator of putting into 3-5 ℃ carries out short-term preservation, during with honeybee, shift to an earlier date 4-7 days and take out and be placed in the indoor cultivation of numerous honeybee from refrigerator, the available expansion of honeybee card after cultivation is numerous or for biological control of insect pests.
4. a kind of method of indoor propagation Trichogramma pintoi according to claim 3, is characterized in that: described rice moth receptacle control indoor temperature is 25-28 ℃, and relative moisture is 60-85 %.
5. a kind of method of indoor propagation Trichogramma pintoi according to claim 3, is characterized in that: it is 25-28 ℃ that indoor temperature is controlled in described numerous honeybee chamber, and relative moisture is 70-85 %.
6. a kind of method of indoor propagation Trichogramma pintoi according to claim 3, is characterized in that: described breeding bee tube is the Boiling tube of diameter 30 mm × length 20 cm.
7. a kind of method of indoor propagation Trichogramma pintoi according to claim 3, is characterized in that: described rice moth egg card is that the sticky rice moth egg of the strip label card of 2 cm × 4 cm is made.
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| CN110973074A (en) * | 2020-01-10 | 2020-04-10 | 夏鹏亮 | Breeding method and device for scale insects and living feed for natural enemy insects |
| CN111226872A (en) * | 2020-03-19 | 2020-06-05 | 湖南省林业科学院 | Method for breeding cotton bollworm tooth-lipped ichneumon fly in large quantity indoors |
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