CN111226872A - Method for breeding cotton bollworm tooth-lipped ichneumon fly in large quantity indoors - Google Patents
Method for breeding cotton bollworm tooth-lipped ichneumon fly in large quantity indoors Download PDFInfo
- Publication number
- CN111226872A CN111226872A CN202010197918.5A CN202010197918A CN111226872A CN 111226872 A CN111226872 A CN 111226872A CN 202010197918 A CN202010197918 A CN 202010197918A CN 111226872 A CN111226872 A CN 111226872A
- Authority
- CN
- China
- Prior art keywords
- cotton bollworm
- breeding
- tooth
- bee
- cotton
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention discloses a method for breeding a large number of cotton bollworm tooth-lipped ichneumonid indoor, belonging to the technical field of biological cultivation. The invention discloses a method for breeding a large number of cotton bollworm tooth-lipped ichneumon fly indoors, which comprises a single-end bee-inoculating method and a colony bee-inoculating method; a large amount of the Helicoverpa armigera Goniorrhiza Georgi bees are obtained by combining the colony grafting method and the single-head grafting method so as to meet the requirements of scientific research and field application of the Helicoverpa armigera Goniorrhiza Georgi bees.
Description
Technical Field
The invention relates to the technical field of biological cultivation, in particular to a method for breeding a large number of cotton bollworm tooth-lipped ichneumonid indoor.
Background
The harm of the plant diseases and insect pests can cause great reduction of the quality and the yield of the tobacco and seriously harm the production and the safety of the tobacco, so the prevention and the treatment of the plant diseases and insect pests of the tobacco are an important part in the production of the tobacco. The oriental tobacco budworm (Heliothis assulata), black cutworm (Agrostis subsilon (Rottemperg)), Prodenia litura (Fabricius) and myzus persicae (Sulzer) are 4 major pests in the province of Hunan province; wherein the oriental tobacco budworm, the black cutworm and the prodenia litura all occur for 4-5 generations in one year. The Helicoverpa armigera (Camptolichchlerideae Uchida) is the dominant parasitic natural enemy of the tobacco budworm and Helicoverpa armigera, and can also parasitize the important leaf-eating pests such as prodenia litura and black cutworm. After being parasitized by the Helicoverpa armigera Ching, the host dies.
Under natural conditions, because the cotton bollworm tooth-lip ichneumon fly and the host thereof show obvious hysteresis, namely when the host larva peak period occurs in the field, the density of the worm mouth of the cotton bollworm tooth-lip ichneumon fly is too low; when the host larva begins to descend, the parasitism of the cotton bollworm tooth-labia ichneumoniae enters a peak period. The natural parasitic rate in the field is low, and the field control requirement is difficult to meet, so that the large-scale breeding technology of the cotton bollworm tooth-lipped ichneumonid needs to be researched, and a large amount of the cotton bollworm tooth-lipped ichneumonid is released before the peak period of the host larvae to make up for the shortage of the natural occurrence quantity in the field.
Therefore, the problem to be solved by those skilled in the art is to provide a method for breeding cotton bollworm tooth-lipped ichneumonid in large quantities indoors.
Disclosure of Invention
In view of the above, the invention provides a method for breeding a large number of cotton bollworm tooth-lipped ichneumonid indoor, and a large number of cotton bollworm tooth-lipped ichneumonid are obtained so as to meet the requirements of scientific research and field application of the cotton bollworm tooth-lipped ichneumonid.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for breeding a large amount of Helicoverpa armigera Oncorhynchus in a room comprises a single-head inoculation method and a colony inoculation method;
the single-end bee-connecting method comprises the following specific steps:
(1) breeding cotton bollworms, and selecting larvae of 4-5 days old after hatching for later use;
(2) placing eclosion mating-finished cotton bollworm tooth ichneumon fly in a culture dish, introducing 1 head of the cotton bollworm larvae of 4-5 days old hatched in the step (1), allowing the cotton bollworm larvae to freely lay eggs, taking out the cotton bollworms after 15min, introducing 1 head of new cotton bollworms, and repeating the steps until the cotton bollworm tooth ichneumon fly finishes laying eggs;
(3) the cotton bollworm which is parasitized by the cotton bollworm tooth-lipped ichneumon fly is placed in a single lattice box and continuously added with feed for independent feeding, and the host shows slow growth, slow action and remarkably reduced food intake; the epidermis begins to shrink in 3 and 4 days after the parasitism, and the growth basically stops; after one week, the body color of the parasitic larva turns from green to brown to yellow, and the parasitic larva is shiny and transparent and hardly eat; 1-3 days before pupation, an aged parasitic wasp larva can creep in the host from the back of the host, and the whole host insect body is dead and stays at leaf surface bract branches and the like; then, the old larva bites the wall of the host body and drills out, a gray cocoon is formed beside the old larva, and the host worm body is shrunken and contracted into a white hollow skin;
(4) placing the cotton bollworm tooth-like hypsizigus marmoreus cocoons into an artificial climate box for feeding, after emergence, mating female and male, and then feeding eggs into the next generation;
the specific steps of the colony bee inoculation method are as follows:
1) feeding armyworms, selecting active armyworms of 3-5 days old, placing in a fresh-keeping box for later use at multiple heads, simultaneously adding feed, inoculating 1 head of female bees which just mate, and placing for 24 h; then the female bees are transferred out and placed in a new multi-head armyworm preservation box, and second batch inoculation is carried out until the female bees die;
2) the parasitic armyworms are placed in a preservation box, the feed is added into the preservation box, normal feeding is continued, and hosts grow slowly, act slowly and eat remarkably reduces; the epidermis begins to shrink in 3 and 4 days after the parasitism, and the growth basically stops; after one week, the body color of the parasitic larva turns from green to brown to yellow, and the parasitic larva is shiny and transparent and hardly eat; 1-3 days before pupation, an aged parasitic wasp larva can creep in the host from the back of the host, and the whole host insect body is dead and stays at leaf surface bract branches and the like; then, the old larva bites the wall of the host body and drills out, a gray cocoon is formed beside the old larva, and the host worm body is shrunken and contracted into a white hollow skin;
3) the Helicoverpa armigera tooth-like Brazilian cocoons are placed in an artificial climate box for breeding, and after emergence, female and male mating is carried out to lay eggs for breeding in the next generation.
When the population of the cotton bollworm tooth-lipped ichneumon fly is small, a single-head bee-grafting method is recommended, so that the parasitic rate can be increased and the number of offspring can be ensured; when a large number of breeding groups are required to be propagated, a group bee-inoculating method is recommended, a large number of cotton bollworm tooth labiatae bees can be obtained in a short time, and although the single-headed bees are possibly inferior to the single-headed bee-inoculating method in the ability of parasitizing lepidoptera larvae, the time can be saved and the propagation can be rapidly expanded. Namely, the parasitic capacity of the individual is reduced while the total amount is ensured, but the total amount can be greatly expanded and improved.
According to the technical scheme, compared with the prior art, the invention discloses a method for breeding a large number of cotton bollworm tooth-lipped ichneumonid indoor, and the large number of cotton bollworm tooth-lipped ichneumonid are obtained by combining a colony bee-grafting method and a single-head bee-grafting method, so that the requirements of scientific research and field application of the cotton bollworm tooth-lipped ichneumonid are met.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a flow chart of a single-head bee-joining method according to the present invention;
wherein, A: breeding cotton bollworms; b: inoculating the bee at one end to lay eggs; c: parasitic helicoverpa armigera larvae; d: selecting the parasitic cotton bollworms, and independently feeding the cotton bollworms in a single compartment; e: the cotton bollworm tooth-labrass bee pupae is separated from the cotton bollworm body; f: the cotton bollworm tooth-like beehive pupae are feathered out after cocooning; g: mating the imagoes of the Gossypia hirsuta and then laying eggs;
FIG. 2 is a flow chart of the group bee-inoculating method technique of the present invention;
wherein, A: feeding armyworm; b: inoculating the bee colony to lay eggs; c: selecting parasitic armyworms; d: feeding parasitic armyworms; e: the cotton bollworm tooth-lipped bee pupae are separated from the armyworm body and cocoon forming is completed; f: placing the parasitic armyworms in a single compartment for independent feeding to finish cocooning; g: mating the cotton bollworm with the hypeoplasia dentalis after the cotton bollworm is bee-bred into imagoes.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The Helicoverpa armigera Ching was collected from the Hunan agriculture university base of Changsha, Hunan, of Hunan, in 2018, 4-8 months. The cotton bollworms and armyworms are artificially fed for many generations by a breeding center of natural enemies in Hunan province.
The method comprises the steps of continuously feeding 3-4 generations of cotton bollworms and armyworms indoors by using a self-made artificial feed as a substitute feed for the Helicoverpa armigera Okayata, and continuously feeding 4-5 days old cotton bollworms for later use. The indoor feeding conditions are that the temperature is 27 +/-1 ℃, the relative humidity is 70 +/-5%, and the illumination period L: D is 14: 10.
Example 1
A method for breeding a large amount of Helicoverpa armigera Oncorhynchus in a room comprises a single-head inoculation method and a colony inoculation method;
the single-end bee-connecting method comprises the following specific steps:
(1) purchasing the cotton bollworm and the feed thereof, normally feeding the cotton bollworm, and selecting larvae of 4-5 days old after hatching for later use;
(2) placing eclosion mating-finished cotton bollworm tooth ichneumon fly in a culture dish, introducing 1 head of the cotton bollworm larvae of 4-5 days old hatched in the step (1), allowing the cotton bollworm larvae to freely lay eggs, taking out the cotton bollworms after 15min, introducing 1 head of new cotton bollworms, and repeating the steps until the cotton bollworm tooth ichneumon fly finishes laying eggs;
(3) the cotton bollworm which is parasitized by the cotton bollworm tooth-lipped ichneumon fly is placed in a single lattice box and continuously added with feed for independent feeding, and the host shows slow growth, slow action and remarkably reduced food intake; the epidermis begins to shrink in 3 and 4 days after the parasitism, and the growth basically stops; after one week, the body color of the parasitic larva turns from green to brown to yellow, and the parasitic larva is shiny and transparent and hardly eat; 1-3 days before pupation, an aged parasitic wasp larva can creep in the host from the back of the host, and the whole host insect body is dead and stays at leaf surface bract branches and the like; then, the old larva bites the wall of the host body and drills out, a gray cocoon is formed beside the old larva, and the host worm body is shrunken and contracted into a white hollow skin;
(4) placing the cotton bollworm tooth-like hypsizigus marmoreus cocoons into an artificial climate box for feeding, after emergence, mating female and male, and then feeding eggs into the next generation; see figure 1 for a technical flow.
The specific steps of the colony bee inoculation method are as follows:
1) purchasing armyworm and armyworm feed, feeding the armyworm, selecting active armyworm of 3-5 days old, placing in a preservation box for later use, adding feed, inoculating 1 female bee after mating, and placing for 24 h; then the female bees are transferred out and placed in a new multi-head armyworm preservation box, and second batch inoculation is carried out until the female bees die;
2) the parasitic armyworms are placed in a preservation box, the feed is added into the preservation box, normal feeding is continued, and hosts grow slowly, act slowly and eat remarkably reduces; the epidermis begins to shrink in 3 and 4 days after the parasitism, and the growth basically stops; after one week, the body color of the parasitic larva turns from green to brown to yellow, and the parasitic larva is shiny and transparent and hardly eat; 1-3 days before pupation, an aged parasitic wasp larva can creep in the host from the back of the host, and the whole host insect body is dead and stays at leaf surface bract branches and the like; then, the old larva bites the wall of the host body and drills out, a gray cocoon is formed beside the old larva, and the host worm body is shrunken and contracted into a white hollow skin;
3) placing the cotton bollworm tooth-like hypsizigus marmoreus cocoons into an artificial climate box for feeding, after emergence, mating female and male, and then feeding eggs into the next generation; see figure 2 for a technical flow.
The results of comparing the single-head bee-inoculating method with the colony bee-inoculating method are shown in Table 1.
TABLE 1 comparison of Single-head bee-joining method and group bee-joining method
The results in Table 1 show that under the conditions that the temperature is 27 +/-1 ℃ and the humidity is 70 +/-5%, the cotton bollworm single-head bee-grafting method and the armyworm colony bee-grafting method only have obvious difference in the cocoon-obtaining rate, and the difference of other parameters is not obvious. From the cocoon weight, the difference of more than 20mg compared with about 15mg reported in the literature is remarkable, and the population individuals bred in the laboratory have certain superiority.
1/3 in the Helicoverpa armigera Oncorhynchus colony adopts a single-head bee-inoculating method, 2/3 adopts a colony bee-inoculating method, and the Helicoverpa armigera Oncorhynchus colony is continuously bred for three generations in a laboratory. The bollworm tooth-lipped ichneumonid was continuously raised in the laboratory for three generations, as shown in table 2.
TABLE 2 continuous rearing of Helicoverpa armigera Onck in laboratory for three generations
The alternate inoculation method of the single-head bee inoculation method and the colony bee inoculation method is adopted, so that the continuation of the colony can be ensured, and the propagation of the colony can be ensured.
The invention further develops the biological characteristic research of the cotton bollworm tooth-lipped ichneumonid, and determines the feeding, mating and spawning habits of the cotton bollworm tooth-lipped ichneumonid in the indoor and the field and the influence of different environmental factors on the biological characteristics of the cotton bollworm tooth-lipped ichneumonid: the total number of wild collected odontoglossum horneri cocoons is 126, and the indoor emergence rate reaches 92.86%. The eclosion rate of the insect in the illumination period (6: 00-18: 00) is high and accounts for 83.3% of the total eclosion amount. The mating success rate is high and has no significant difference when the emergence peak period is between 6:00 and 8:00 in the morning and between 20 and 30 ℃; mating success rate was highest at 1500 LX. The average single-female egg laying amount of the cotton bollworm tooth-labia ichthyophthirius is 81.58 +/-16.3 grains under the conditions of the temperature of 22 ℃ and the illumination of 1500 LX; the larva has moderate growth course and high survival rate under the conditions of 27 +/-1 ℃ and RH70 +/-5%.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (2)
1. A method for breeding a large number of cotton bollworm tooth-lipped ichneumon fly indoors is characterized by comprising a single-head bee-inoculating method and a colony bee-inoculating method;
the single-end bee-connecting method comprises the following specific steps:
(1) breeding cotton bollworms, and selecting larvae of 4-5 days old after hatching for later use;
(2) placing eclosion mating-finished helicoverpa armigera into a culture dish, introducing 1 cotton bollworm of 4-5 days old after hatching in the step (1), allowing the cotton bollworm to freely lay eggs, taking out the cotton bollworm after 15min, introducing 1 new cotton bollworm, and repeating the steps until the cotton bollworm helicoverpa armigera finishes laying eggs;
(3) placing the cotton bollworms parasitized by the cotton bollworm acromioculcas sempervirens in a single-lattice box, and continuously and independently breeding until mature larvae of the cotton bollworm acromioculcas semens are drilled out and form grey white cocoons;
(4) placing the cotton bollworm tooth-like hypsizigus marmoreus cocoons into an artificial climate box for feeding, after emergence, mating female and male, and then feeding eggs into the next generation;
the specific steps of the colony bee inoculation method are as follows:
1) feeding armyworms, selecting active armyworms of 3-5 days old, placing in a culture container at multiple heads for later use, adding feed, inoculating 1 head of female bees which just mate, and placing for 24 h; then the female bees are transferred out and placed in a new multiheaded armyworm culture container, and second batch inoculation is carried out until the female bees die;
2) placing the parasitized armyworms in a culture container, and continuously breeding until mature larvae of the Helicoverpa armigera tooth-like labrass are drilled out to form grey white cocoons;
3) the Helicoverpa armigera tooth-like Brazilian cocoons are placed in an artificial climate box for breeding, and after emergence, female and male mating is carried out to lay eggs for breeding in the next generation.
2. The method for indoor mass rearing of Helicoverpa armigera Lamarck according to claim 1, wherein the number of the multiple head armyworms in the colony grafting method is 10.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010197918.5A CN111226872A (en) | 2020-03-19 | 2020-03-19 | Method for breeding cotton bollworm tooth-lipped ichneumon fly in large quantity indoors |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010197918.5A CN111226872A (en) | 2020-03-19 | 2020-03-19 | Method for breeding cotton bollworm tooth-lipped ichneumon fly in large quantity indoors |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111226872A true CN111226872A (en) | 2020-06-05 |
Family
ID=70863158
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010197918.5A Pending CN111226872A (en) | 2020-03-19 | 2020-03-19 | Method for breeding cotton bollworm tooth-lipped ichneumon fly in large quantity indoors |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111226872A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114190338A (en) * | 2022-01-20 | 2022-03-18 | 河南农业大学 | Indoor propagation method and application of Helicoverpa armigera Okayata |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1273027A (en) * | 2000-06-15 | 2000-11-15 | 河北省农林科学院植物保护研究所 | Artificial reproduction method for Microplitis mediator (Haliday) |
| CN1511450A (en) * | 2002-12-31 | 2004-07-14 | 杨忠岐 | Biological preventing and controlling of American white moth by chouioia cunea yang |
| CN1647626A (en) * | 2005-01-26 | 2005-08-03 | 北京市农林科学院 | Artificial breeding and producing method for trichogramma |
| CN103782969A (en) * | 2014-03-03 | 2014-05-14 | 广西壮族自治区林业科学研究院 | Method for breeding trichogramma pintoi indoors and corcyra cephalonica raising fodder |
| WO2018178870A1 (en) * | 2017-03-31 | 2018-10-04 | Fundacion Universidad De Bogota Jorge Tadeo Lozano | Method for rearing and selecting pupae of encarsia formosa and amitus fuscipennis and method for controlling whitefly in greenhouse tomato crops |
-
2020
- 2020-03-19 CN CN202010197918.5A patent/CN111226872A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1273027A (en) * | 2000-06-15 | 2000-11-15 | 河北省农林科学院植物保护研究所 | Artificial reproduction method for Microplitis mediator (Haliday) |
| CN1511450A (en) * | 2002-12-31 | 2004-07-14 | 杨忠岐 | Biological preventing and controlling of American white moth by chouioia cunea yang |
| CN1647626A (en) * | 2005-01-26 | 2005-08-03 | 北京市农林科学院 | Artificial breeding and producing method for trichogramma |
| CN103782969A (en) * | 2014-03-03 | 2014-05-14 | 广西壮族自治区林业科学研究院 | Method for breeding trichogramma pintoi indoors and corcyra cephalonica raising fodder |
| WO2018178870A1 (en) * | 2017-03-31 | 2018-10-04 | Fundacion Universidad De Bogota Jorge Tadeo Lozano | Method for rearing and selecting pupae of encarsia formosa and amitus fuscipennis and method for controlling whitefly in greenhouse tomato crops |
Non-Patent Citations (1)
| Title |
|---|
| 杨赛赛等: "棉铃虫齿唇姬蜂人工繁育形态标准探讨", 《河南科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114190338A (en) * | 2022-01-20 | 2022-03-18 | 河南农业大学 | Indoor propagation method and application of Helicoverpa armigera Okayata |
| CN114190338B (en) * | 2022-01-20 | 2022-11-25 | 河南农业大学 | Indoor propagation method and application of Helicoverpa armigera Okayata |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5543583B2 (en) | How to cultivate orange scallop scallop | |
| CN102349462A (en) | Breeding method for improved variety of fishes | |
| CN113016673A (en) | Method for producing oyster of Fujian oyster of yellow shell/black shell triploid in large scale | |
| CN103004660A (en) | Parent fish culturing method for scaled artificial breeding of finless ell | |
| CN113287574B (en) | Method for propagating blue Changcongjin wasp | |
| CN109275600B (en) | Indoor seedling raising method for mustache obliqua | |
| CN105284682B (en) | A kind of breeding method of hippocampus artificial propagation parent | |
| CN111226872A (en) | Method for breeding cotton bollworm tooth-lipped ichneumon fly in large quantity indoors | |
| Wyatt | The distribution of Myzus persicae (Sulz.) on year‐round chrysanthemums: II. Winter season: the effect of parasitism by Aphidius matricariae Hal. | |
| CN110679555B (en) | Breeding method of artificial feed adaptive silkworm variety | |
| Ajah | Mass culture of Rotifera(Brachionus quadridentatus[Hermann, 1783]) using three different algal species | |
| CN110074019B (en) | Stereo ecological breeding method for takifugu obscurus, coilia ectenes and soft-shelled turtles | |
| CN114651793B (en) | Artificial breeding of trichogramma obscura and method for preventing and treating spodoptera frugiperda by using trichogramma obscura | |
| CN101292633B (en) | Bredding method for hybridization of fish-Ji'ao tilapia | |
| CN104823923B (en) | A kind of mating system for preventing aphidius gifuensis population deterioration | |
| CN111066732B (en) | Method for breeding orius sauteri | |
| CN107455341A (en) | A kind of aphidius gifuensis mating system of regulation and control host crop growth | |
| CN113826588B (en) | Indoor breeding method for spodoptera litura lateral groove bombesi | |
| CN106900657A (en) | A kind of method that use live body host plant expands numerous black striped plant bug | |
| CN112471082A (en) | Broad-band optimized aphid fly breeding method | |
| CN106719443B (en) | Method for breeding brown sandflies by utilizing aphids | |
| CN111727936A (en) | Indoor propagation method of chilo suppressalis fringed cocoon bee | |
| CN110839596B (en) | Method for breeding male bees of young yellow Encarsia aphidicola by using aphidius gifuensis | |
| CN108935347B (en) | Breeding method of griseofulvin | |
| CN110367207B (en) | Method for breeding large amount of trichogramma matsutake with convex legs |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |