CN105284682B - A kind of breeding method of hippocampus artificial propagation parent - Google Patents

A kind of breeding method of hippocampus artificial propagation parent Download PDF

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CN105284682B
CN105284682B CN201510698912.5A CN201510698912A CN105284682B CN 105284682 B CN105284682 B CN 105284682B CN 201510698912 A CN201510698912 A CN 201510698912A CN 105284682 B CN105284682 B CN 105284682B
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hippocampus
parent
female
artificial propagation
temporarily
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CN105284682A (en
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徐永健
牟金婷
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Ningbo University
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Ningbo University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention discloses a kind of breeding method of hippocampus artificial propagation parent, feature is to comprise the following steps:(1)The selection of parent:Select that body surface is clean, big, sturdy active, the artificial farming hippocampus that ingestion ability is strong of individual is as artificial propagation hippocampus parent;(2)Parent is temporarily supported:The hippocampus parent selected temporarily is supported in indoor brood pond, feeds chilled or live body oppossum shrimp as bait, and daily feeding 45 times, temporarily foster environmental condition be salinity 24 25, and 26 DEG C of temperature 24 DEG C, white light or blue light are photochromic, and light intensity is 40 × 102Lx, the 40dB of background sound 30, the 75cm of the depth of water 70, attachment are natural plants sand filter seawater;Hippocampus parent male and female are isolated and cultivated, cultivation density is that 2 heros are 2 female, when hippocampus body colour change and choose spouse behavior when, then male and female hippocampus is mixed, advantage is to obtain optimal artificial propagation effect, can improve the farrowing amount of parent hippocampus and the survival rate of young hippocampus.

Description

A kind of breeding method of hippocampus artificial propagation parent
Technical field
The present invention relates to agriculture technical field of aquaculture, more particularly to a kind of cultivation side of hippocampus artificial propagation parent Method.
Background technology
Recent decades, aquaculture technology and method are more and more important, not only due to it is its commercial value, it is more important Be as a kind of instrument of plasm resource protection.For meet to globalize resource maintenance and the needs of environment remediation, improve and support Plantation technology and method are extremely urgent.Hippocampus is as a kind of precious marine organisms, due to its high medical value and uniqueness Profile, become health-product market and view and admire the favorite in market.Wild hippocampus causes quantity to be greatly reduced due to overfishing, It is put into IUCN and Chinese endangered animal and plant protection Red List as endangered species.It is artificial to support in order to solve this bottleneck Growing expansion hippocampus resource turns into a kind of effective measures.Though there are many countries to carry out the research of related fields, but still deposit In some technical problems, the problem of parents culture method for being mainly manifested in hippocampus is not captured also, and young survival rate is low, is urgently Solve, it is of poor benefits, it is difficult to form large-scale cultivation production.Therefore, it is badly in need of a kind of hippocampus parents culture method of standardization, meets The demand of hippocampus artificial farming, expand the quantity of hippocampus resource, so as to protect wild hippocampus resource, and then create considerable economy Value.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of farrowing amount for improving parent hippocampus and young hippocampus The breeding method of the hippocampus artificial propagation parent of survival rate.
Technical scheme is used by the present invention solves above-mentioned technical problem:It is a kind of to cultivate supporting for hippocampus artificial propagation parent Method is grown, is comprised the following steps:
(1)The selection of parent:The artificial farming hippocampus that body surface is clean, individual is big, sturdy vivaciously, ingestion ability is strong is selected to make For artificial propagation hippocampus parent;
(2)Parent is temporarily supported:The hippocampus parent selected temporarily is supported in indoor brood pond, feeds chilled or live body oppossum shrimp as bait Material, daily feeding 4-5 time, it is salinity 24-25 temporarily to support environmental condition, and 24 DEG C -26 DEG C of temperature, white light or blue light are photochromic, and light intensity is 40×102Lx sand filter seawaters;It is that 2 heros 2 are female by hippocampus parent male and female cultivation density, and male and female isolation culture, treat that hippocampus body colour is sent out Changing and choose spouse behavior when, then by male and female hippocampus be mixed.
Hippocampus parent selects a rheological properties maturation hippocampus or two age hippocampus for amixia history, and specification requirement is grown for body>11.0 Cm, body weight>3.2 g, appearance requirement are that skin and organ are complete, and no wound, body colour is based on black, dark brown.
Temporarily foster temperature is 26 DEG C.The reproductive efficiency highest of the temperature Hippocampus Kuda, optimal litter size(293.7± 15.54ind/ tails)With young survival rate(48.55±5.09%), growth cycle is about 27.83 ± 0.75 days.
Live body oppossum shrimp is fed as bait.Chilled and live bait can be such that artificial propagation hippocampus parent normally produces, live bait compared with Chilled bait can make production shift to an earlier date 10-15 days, and the live bait group production cycle is relatively stable;Live bait group farrowing amount(283.5± 50.18 ind/ tails)It is significantly higher than chilled bait group(246.8 ± 39.06 ind/ tails), two groups of young survival rates are similar.
Described hippocampus parent cultivation depth is 65-75cm below the water surface.The Mating success of the depth hippocampus is higher can Up to 100%, reproductive efficiency is high, and young farrowing amount is up to 253.7 ± 15.54ind/ tails.
The room noise that described hippocampus parent is temporarily supported is 30-40dB.Sound has certain for the mating behavior of hippocampus Influence, under faint acoustic background, Mating success is high.
The twister of described hippocampus parent is natural plants.Hippocampus rest is often attached on some objects, and hippocampus is with day Right plant is attachment, and its twine rate is 53.28 ± 18.57%.Contribute to hippocampus normally to rest, flush, to farrowing There is facilitation.
Compared with prior art, the advantage of the invention is that:The invention discloses a kind of training of hippocampus artificial propagation parent Method is educated, this method is cultivated using male and female isolation, with 2 male 2 female pairings, feeds living body bait, temperature is kept for 26 DEG C, gives white light Or blue light, light intensity is 40 × 102During Lx, background sound 30-40dB, depth of water 70-75cm, attachment is natural plants, energy Optimal artificial propagation effect is obtained, the farrowing amount of parent hippocampus and the survival rate of young hippocampus can be greatly improved.
Embodiment
The present invention is described in further detail with reference to embodiments.
Specific embodiment one
A kind of cultural method for cultivating hippocampus artificial propagation parent, comprises the following steps:
(1)Select that body surface is clean, big, sturdy active, the artificial farming hippocampus that ingestion ability is strong of individual is as artificial propagation Parent:A, a rheological properties maturation hippocampus of amixia history(Specification requirement:Body is grown>11.0 cm, body weight>3.2 g)Or two ages of selection Hippocampus.B, outward appearance:Skin and organ are complete, and no wound, body colour is based on black, dark brown;
(2)Parent is temporarily supported:The hippocampus parent selected temporarily is supported in indoor brood pond, feeds chilled or live body oppossum shrimp as bait Material, temporarily foster environmental condition is salinity 24-25, and 24 DEG C -26 DEG C of temperature, white light or blue light are photochromic, and light intensity is 40 × 102The husky filter seas of Lx Water, twister when the interior thin bamboo branch of placement of brood pond is as hippocampus rest;It is that 2 heros 2 are female and female by hippocampus parent male and female cultivation density Hero isolation culture, when hippocampus body colour change and choose spouse behavior when, then male and female hippocampus is mixed.
The screening and daily management of hippocampus parent, big, the sturdy parent active, ingestion ability is strong of selection individual, body length> 11.0cm body weight>3.2 g.Feeding 4-5 times daily, water quality will keep cleaning, and dissolved oxygen is sufficient, and day and night temperature is no more than 2-3 DEG C. Some bamboo branch are put in culture pond as hippocampus rest twister.
Specific embodiment two
Temperature optimization
In order to determine suitable parents culture temperature, it is respectively 22 DEG C, 26 DEG C, 30 DEG C there is provided 3 groups of humid test groups, enters The bimestrial experiment of behavior phase.The main average farrowing amount that have selected parent hippocampus, 30 days young survival rates, the production frequency, life The parameters such as long period are as measurement index.When the results are shown in Table 1,22 DEG C, parent hippocampus produces once, and production starts from mid-June; At 26 DEG C, parent hippocampus produces twice, produces since mid or late May;At 30 DEG C, parent hippocampus produces three times, produces from May In the middle ten days, as a result show, at a suitable temperature, production number increases with the rise of temperature, meanwhile, the date of manufacture Shift to an earlier date with the rise of temperature(Production cycle shortens).As the humid test group of table 1 three farrowing amount and 30 days young into Shown in motility rate situation, temperature has no effect on the normal mating of parent hippocampus, but influences the number of mating.In 26 DEG C of second of productions In, the farrowing amount highest of parent hippocampus(293.7 ± 15.54 ind/ tails), the survival rate of the young in 30(48.55 ± 5.09%) And highest.So if pursuing the situation with a batch of young, 26 DEG C are advisable, if single requirement output Words, can select 30 DEG C to be used as cultivation temperature.
Influence of the temperature of table 1 to hippocampus farrowing amount and survival rate
Specific embodiment three
Bait selects
Have selected two groups of bait is respectively live bait oppossum shrimp and Penaeus Vannmei son shrimp group, chilled oppossum shrimp group, is scheduled to last Bimestrial experiment.Selected production number, production time, average farrowing amount, the parameter such as young survival rate is standard in 30 days.Knot Fruit is shown in Table 2, and two groups of bait, the production number on parent hippocampus does not influence, and living bait group was produced since late May, continues To by the end of June;Chilled bait group production starts from early June, continues to mid or late July, compares, chilled bait group produces some and pushed away Afterwards, the production cycle extends.Shown in the influence situation produced such as 2 different bait of table to parent hippocampus, chilled bait group is in third time Only have 2 pairs of parent hippocampus to mate successfully in production, other 4 couples occur not producing phenomenon.Fresh food group farrowing amount is higher than chilled Bait group.But from the point of view of the survival rate of the young in 30 days, chilled group is slightly above fresh food group.
Influence of the 2 different bait of table to hippocampus farrowing amount and young survival rate
Specific embodiment four
Male and female cultivation density optimizes
In order to determine most suitable parent cultivation density, according to the limitation of the conditions such as breeding spaces, 2 groups of density examinations are devised Test, respectively 1 female 1 male group(A groups), 2 female 2 male groups(B groups), carried out bimestrial experiment by a definite date(5 ~ July).Selected production time Number, production time, average farrowing amount, the parameter such as young survival rate is standard in 30 days.As a result show, two groups from late May Start to produce, at the beginning of continueing to 7 months.Two groups produce twice, and B groups mating number of success is far above A groups.Such as the different densities of table 3 Shown in influence to hippocampus production, two experimental groups, second of farrowing amount produced is above first time output, in survival rate side Face does not have notable difference.
Influence of the different densities of table 3 to hippocampus farrowing amount and young survival rate
Specific embodiment five
Male and female breeding way improves
In order to probe into the influence that the breeding way of male and female parent produces to it, male and female mixed breed group (MG) and female have selected Male separately cultivation group (SG), when observe mixing group choose spouse mating behavior after, be converted to mixed culture by separated group, progress Bimestrial experiment by a definite date(The 5-7 months).Selected production number, production time, average farrowing amount, the ginseng such as young survival rate in 30 days Number is standard.As a result show, in terms of body colour, separate group and have almost no change, based on black, dark brown, the Zhou Houti of mixing group one Color gradually turns yellow, is even complete yellow.Body grows and body weight indifference.As the male and female difference training method of table 4 influences on the hippocampus condition of production Shown, the farrowing amount for separately organizing parent hippocampus is significantly higher than mixing group, and the survival rate of the young in 30 days, separated group is also significantly greater than Mixing group.
Influence of the male and female difference training method of table 4 to hippocampus farrowing amount and young survival rate
Specific embodiment six
Property when matches the optimization of specification
Property than the mating for hippocampus and spouse screening have important influence.In order to which the property for determining optimal when cultivates rule The dispensing of lattice, we devise three groups of property and are respectively than experiment sex rations(1:1;1:3;3:1).As a result as shown in table 5,3:1 group It is slightly above other two groups, while 1:3 to assemble to chance of success be to have certain relation with the specification of male and female, when different rule being present In the case of lattice, hippocampus tends to select spouse close with oneself specification or larger than oneself.
The dissimilarity of table 5 compares the influence of mating logarithm
Specific embodiment seven
Photochromic optimization
In order to determine the photochromic of the adaptation sensitivity of parent hippocampus, we have selected four kinds of white light, feux rouges, green glow, blue light light Color, the experiment of one month is by a definite date carried out.It has selected specific growth rate(SGR), food ration, the parameter such as the death rate is as standard. As a result show, the specific growth rate highest of white light(5.88±0.52), feux rouges is minimum, and blue light is slightly above green glow.In terms of grazing rate, As a result consistent with growth rate, white light highest, feux rouges is minimum.Death condition, feux rouges highest, it is green glow, white light and blue light successively. It is specific as shown in table 6:
Table 6 is different photochromic to ingest and the influence of the death rate to parent hippocampus
In summary, in white light(1260nm), blue light (440nm), green glow (550nm), the illumination condition of feux rouges (630nm) Under, the grazing rate highest of white light, next to that blue and green light, feux rouges food ration is minimum.In terms of the death rate, feux rouges group>Green glow group >White light group>Blue light group.Mating success of the parent under blue light and white light is higher.
Specific embodiment eight
The optimization of intensity of illumination
In order to determine most suitable intensity of illumination, we set 20 × 102Lx、40×102Lx、80×102Tri- groups of experiments of Lx Group, the experiment of one month by a definite date is carried out, have selected specific growth rate(SGR), food ration, the parameter such as the death rate is as standard. As a result show, 40 groups of SGR highests.In aspect of ingesting, the > 20 of 40 > 80.And 30 days > 40 of 80 > of the death rate 20.Specific such as table 7:
7 different light intensity of table are ingested and dead influence to parent hippocampus
In summary, too strong or excessively weak light intensity all has an impact to hippocampus.20 × 102Lx、40×102Lx、80× 102Under tri- groups of intensities of illumination of Lx, 40 × 102Lx grazing rate highest;Death rate situation in 30 days, 80 × 102The Lx death rates are most It is high.40 × 102Mating success under Lx light intensity is higher.
Specific embodiment nine
The optimization of the depth of water
In order to determine most suitable breeding water body depth, we set tetra- groups of experimental groups of 55cm, 65cm, 75cm, 85cm.Choosing The parameter such as Mating success, young number is determined as standard.As a result show, tri- groups of unobvious difference of 65cm, 75cm, 85cm, in order to Fanning economics, our selected 65-75cm are advisable.As a result display such as table 8:
The influence of mating and farrowing of the different breeding water depth of table 8 to close hippocampus
55 65 75 85
Mating success(%) 40.3±0.45 100 100 100
Young number(Tail) 85±8.34a 183±9.27b 189±6.19b 191±7.23b
Specific embodiment ten
Background sound optimizes:Sound has a certain impact for the mating behavior tool of hippocampus.Set in quiet (10dB), cultivation Standby sound(30-40dB), cultivation equipment adds under the conditions of air blower manufactures sound (70-90dB), it is found that the faint sound back of the body of equipment Under scape, Mating success is high, and air blower manufacture sound can even cause hippocampus dead.
Specific embodiment 11
The optimization of attachment
We set the materials such as natural plants, artificial plant, green linear structure as attachment, as a result such as table 9:
The influence that 9 different attachments of table are rested to parent
Certainly, described above is not limitation of the present invention, and the present invention is also not limited to the example above.The art Those of ordinary skill is in the essential scope of the present invention, the variations, modifications, additions or substitutions made, should also belong to the present invention's Protection domain.

Claims (4)

1. a kind of cultural method for cultivating hippocampus artificial propagation parent, it is characterised in that comprise the following steps:
(1) selection of parent:Select that body surface is clean, big, sturdy active, the artificial farming hippocampus that ingestion ability is strong of individual is as people Work breeds hippocampus parent;
(2) parent is temporarily supported:The hippocampus parent selected temporarily is supported in indoor brood pond, feeds chilled or live body oppossum shrimp as bait, often Its feeding 4-5 times, temporarily foster environmental condition be salinity 24-25, and 24 DEG C -26 DEG C of temperature, white light or blue light are photochromic, light intensity for 40 × 102Lx sand filter seawaters;It is that 2 heros 2 are female by hippocampus parent male and female cultivation density, and male and female isolation culture, treat that hippocampus body colour becomes Change and choose spouse behavior when, then by male and female hippocampus be mixed, wherein hippocampus parent cultivation depth is underwater 65- 75cm;The room noise that hippocampus parent is temporarily supported is 30-40dB;The twister of hippocampus parent is natural plants.
A kind of 2. cultural method for cultivating hippocampus artificial propagation parent according to claim 1, it is characterised in that:Hippocampus parent Body selects a rheological properties maturation hippocampus or two age hippocampus for amixia history, and specification requirement is grown for body>11.0cm, body weight>3.2g, outside See and require complete for skin and organ, no wound, body colour is based on black, dark brown.
A kind of 3. cultural method for cultivating hippocampus artificial propagation parent according to claim 1, it is characterised in that:Step (2) temporarily foster temperature is 26 DEG C in.
A kind of 4. cultural method for cultivating hippocampus artificial propagation parent according to claim 1, it is characterised in that:Step (2) live body oppossum shrimp is fed in as bait.
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106577372B (en) * 2016-11-04 2019-04-16 宁波大学 A kind of overwintering and artificial fecundation method of sea otter
CN106577559B (en) * 2016-11-04 2019-04-16 宁波大学 A kind of fishing and artificial domestication method of sea otter
CN107439428A (en) * 2017-09-04 2017-12-08 天津农学院 A kind of selection of strain line hippocampus high yield family
CN113598092B (en) * 2021-07-13 2023-06-02 福建省水产研究所(福建水产病害防治中心) Cultivation method of ventral sea horse family

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Publication number Priority date Publication date Assignee Title
CN101589692A (en) * 2009-07-01 2009-12-02 宁波大学 The ecological cultivation method of a kind of hippocampus
CN102210278A (en) * 2011-06-17 2011-10-12 中国科学院南海海洋研究所 Method for domesticating and breeding wild hippocampus
CN103548725A (en) * 2013-10-18 2014-02-05 福建港德水产有限公司 Hippocampus kelloggi artificial breeding method
CN104381172A (en) * 2014-11-17 2015-03-04 苏州市相城区盛胡特种养殖专业合作社 Ecological aquaculture method for sea horses

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101589692A (en) * 2009-07-01 2009-12-02 宁波大学 The ecological cultivation method of a kind of hippocampus
CN102210278A (en) * 2011-06-17 2011-10-12 中国科学院南海海洋研究所 Method for domesticating and breeding wild hippocampus
CN103548725A (en) * 2013-10-18 2014-02-05 福建港德水产有限公司 Hippocampus kelloggi artificial breeding method
CN104381172A (en) * 2014-11-17 2015-03-04 苏州市相城区盛胡特种养殖专业合作社 Ecological aquaculture method for sea horses

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