KR20160073534A - Aquaculture Methods for Mottled Eel - Google Patents

Abstract

The present invention relates to a method for aqua-culturing Anguilla marmorata, which is the eel imported from Southeast Asia where resources are abundant and the price of glass eels is low, capable of substituting for glass eels from Far East Asia, of which the natural fishing amount is reduced, the price is high, and the supply and demand is unstable. The method for aqua-culturing imported eels comprises the steps of: fishing glass eels and putting a juice type initial feed thereinto; converting the feeding mode into a mixed feed of an initial feed and a white eel feed; and growing the eels using a white eel feed, a black eel feed, and a young eel feed, wherein in each step, the eels are aqua-cultured while the temperature of aqua-culture water is 32°C, and the eels are fed with a feed amount wherein, the percentage of the feed amount on the basis of the weight of an eel, y, satisfies the following equation: y = 1.5585 ln(x) - 4.4434, here, x is the weight of an eel. Therefore, the present invention can contribute to the promotion of the eel aqua-culture industry.

Description

Aquaculture Methods for Mottled Eel}

The present invention relates to a method for producing foreign eel, and more particularly, it relates to a method for producing foreign eel, which comprises the steps of applying a juice-fed mouthwash using an eel of an eel ( Anguilla marmorata ) The present invention relates to a method for efficiently cultivating foreign eels by providing an optimal rearing temperature and feed amount at each stage of growing white porcelain, surplus and tangerine feed.

Eel species is 16 species and 3 species. 19 species are widely distributed worldwide. In Korea, there are two species of Anguilla japonica and Anguilla marmorata .

Spawning activity and early larval development of eels are not well known yet, and the spawning season is estimated from early spring to summer. Eel spawning sites are known as the 400- to 500-meter water depths in the Pacific Ocean, with temperatures ranging from 16 to 17 degrees Celsius, salinity of more than 35 ‰, north of 20 degrees to 28 degrees N, and east of 121 degrees to 128 degrees east. The eel is a descendant fish, the fry moves gradually from the middle layer to the surface layer, and spreads in various directions by currents. The willow leaf-shaped flesh of this period is called Leptocephalus larvae. Leptosepalus larvae migrate to shallow coastal waters by ocean currents and develop to the level of the eel after settlement on the bottom. Thread eels migrate upstream of the river and settle in the middle of the river, in the lake, in the swamp or in the wastewater station.

Eels are nocturnal, and migratory and feeding behavior is observed only at night in natural habitats. Seedlings raised to the river grow to 300 ~ 1,000g for 4 ~ 12 years, while males mature when they are 3-4 years old and mature when they are 4-6 years old for females. The mature eel returns to the sea for spawning, does not eat food during this period, and is known to die after spawning, but the eel life is not known precisely.

As such, eels are not well known for their life cycle and spawning ecology, making artificial seedling difficult. Since the successful completion of the artificial hatching of eels in Japan in 1976, efforts have been made to produce artificial seeds. In 2012, there have been reports that the artificial hatching of eels and the production of the eel stages have succeeded. However, It is true. Therefore, the eels, which are middle stage of growth, come to rivers and are cultured.

The demand for eels in Korea is about 20 tons (about 10 million), but the catching of eels is not constant every year, so the prices fluctuate so much. It is a situation that gives many difficulties. As a solution, a method of importing Far Eastern wild eel ( Anguilla japonica ) from foreign countries or wearing eel of Anguilla anguilla , Anguilla rostrata from North American or South American eel was suggested.

However, in Europe in 2013, in order to protect the rapidly declining freshwater eel, Anguilla anguilla is classified as an endangered species and all wild collecting and commercial transactions are prohibited. In addition, Far East freshwater eel ( A. japonica ) , The formation of rivers and estuaries, and ecosystem changes, the species has recently been classified as an endangered species by the World Conservation Union.

On the other hand, Anguilla marmorata is a large tropical fish species belonging to the Anguillidae family. It is commonly found in southern China, Taiwan, southern Japan, the Philippines, and Indonesia. However, in Korea, Since only a few objects were found, it was designated as Natural Monument 258 in 1962.

However, in the Southeast Asian region, it was common for food, and it was brought into the country for the purpose of food production, and the designation of the natural monument in 2009 was canceled due to the degradation of the natural monument and the problem of national sentiment. Intrepid eel is bigger than eel (Anguilla japonica), body base is yellowish brown or blackish brown, abdomen is white, and dark brown-black banquet is scattered in whole body. It lives mainly in rivers with relatively high yields and is carnivorous and phagocytic. Therefore, it eats crustaceans, crustaceans and other fishes at random.

Indoor eel is a tropical fish, and it is known that it comes north along the turbulence in Korea. Spawning season and spawning place are not known, and Leptosepalus larvae like the Far Eastern eel. Objects with body lengths of 60 to 120 cm are common, and those over 200 cm are rarely found.

Anguilla marmorata is a high-quality fish that is said to be at a price three times higher than that of ordinary eels in China because of its delicious taste, but also has a large amount of eels in Southeast Asia. It can be put on the farm at the price of one minute.

However, there was no definite aquaculture technology for these Southeast Asian eels, and most of them had to be killed before they became surname. In fact, it is similar to the appearance of the eel of the Far East mountain and the eel of the eel eel of the Southeast Asia, and it is difficult to distinguish it, and some importers try to sell the Eel eels of the Southeast Asian eel to the far eastern eels, Because eel of eel is easy to die, eel of eel eel has stigma called bad eel.

Therefore, there is a need to find a way to utilize the tropical eel marmorata ( Anguilla marmorata ), which has abundant resources and low price of eel, to cope with supply-demand imbalance in the Far East mountain eel and to develop the Yangmang industry continuously. Do.

Japanese Patent Application Laid-Open No. 10-2014-0115445 discloses a method of manufacturing a descending fish including a step of transporting a fish, a step of breeding a fish, and a step of moving a fish, wherein a water current is generated using a submersible motor having a venturi, Wherein the step of cultivating the fish comprises the step of cultivating the fish in a reverse direction of the water flow and allowing the fish to flow without feeding for 30 to 40 days. In Korean Patent No. 10-1212399, the composition of the food chain is divided into three stages. The first food chain composition is disinfected by spreading lime at 250-350 g / m 2 in tiller farmland or idle land, and 2- The second food chain composition consists of cultivating daphnia to connect the food chain to the food chain and to connect the food chain to the third food chain, The composition is prepared by breeding shrimp, small fish and the like and connecting the food chain linkage with the second food chain composition stage, and then feeding the larvae to the breeding eel, which is the predator of the highest order, in the formed food chain linkage. And discloses a method of eco-friendly eel ecological cultivation using a composition. In Korean Patent No. 10-1316450, 3 to 8% by weight of skim milk powder is added to mushroom extract extracted under a certain condition, homogenized with a blender for 3-7 minutes, and sterilized at 90 ° C for 1-2 hours; After sterilization in the above step, the mixture is allowed to cool to about 37 to 40 DEG C, the lactic acid bacteria stator is inoculated at 1% (V / V) and fermented at 37 DEG C for a certain period of time; Preparing fermented milk by mixing fermented milk of fermented mushroom-extracted lactic acid bacteria with a compounded feed; And maintaining the water temperature of the cultivation water in the culture tank of both ten thousand cultures at a constant level, while feeding the paste feed to the feed of eels raised in a water tank, wherein the physiologically active substance is used as a feed additive . Domestic registered patent No. 10-1195353 uses ground underground soil removed from the surface layer by about 1 m and mixed with ground water coming out from the ground at a depth of 150 m for at least 24 hours and left to stand for 3 days to make yellow clay groundwater. The worms were separated from pure earthworms in order to produce fish worms and red earthworms in the form of fish worms. The worms were heated for 3 hours in a boiler at 550 ℃. And then subjecting the eel to a feed prepared by kneading the earthworm liquid and the compound feed with the yellow algae supernatant, and then feeding the eel and the earthworm, A method for cultivating eels is disclosed. These prior arts, however, are related to the step of putting Far Eastern eels on the market or to provide a food composition, in order to apply the foreign eel (Anguilla marmorata) disclosed in the present invention and to optimize the optimum water temperature The purpose, composition and effect of eel culture methods differ from those of foreign eel culture methods that provide feeds.

Although the present invention has not yet established an optimized aquaculture method in order to cope with the imbalance in supply and demand of Far East mushroom eels and to continue the development of Yangmansan industry, it has been found that Southeast Asian Anguilla marmorata , rich in resources and low in eel cost, To provide a new method of cultivation for cultivation.

The present invention relates to a process for preparing an eel of a foreign eel ( Anguilla marmorata ), comprising the steps of administering a juice-fed mouthwash, a step of converting the mouthwash and white meal into a mixture feed, a white meal, a surplus meal, The feed rate of the feed amount to the number of eels / kg is x, and the feed rate of the feed amount to the number of eels / kg is expressed as y = 1.5585 ln (x) -4.4434. The present invention provides a method for culturing an eel.

According to the method of foreign eel production according to the present invention, it is possible to reduce the mortality rate of foreign eel which has been killed easily by raising the optimum amount of feed while culturing at the optimum temperature, It is possible to substitute for the Far Eastern mountain eel which is soaring and unstable supply, and it is possible to cultivate the Yangyang industry steadily, and it can contribute to increase income of farmers.

FIG. 1 is a photograph showing a test water tank and an entrance of a foreign eel in an initial stocking stage according to the present invention.
FIG. 2 is a photograph showing a cold temperature controller and a water temperature automation facility for controlling an experimental water temperature according to the present invention.
FIG. 3 is a photograph showing the quality of the feed water after feeding and the water after feeding in the initial stocking stage according to the present invention. A: Initial nappy feed grade B: Mixed feed grade C: Feeding condition after feeding.
FIG. 4 is a chart showing the feeding rate of the eel prepared according to the test period and the growth stage of the initial stocking stage according to the present invention.
FIG. 5 is a graph showing the total weight change of each section during the rearing period of the initial stocking stage.
FIG. 6 is a graph showing the average weight change of each section during the rearing period of the initial stocking stage according to the present invention.
FIG. 7 is a graph showing changes in feed efficiency of each section during the rearing period of the initial stocking stage according to the present invention.
8 is a graph showing the survival rate of each section during the rearing period of the initial stocking stage according to the present invention.
FIG. 9 is a photograph showing Southeast Asian eel in a state of being wrapped for the measurement of the middle ear production step according to the present invention.
FIG. 10 is a graph showing changes in the total weight of each section during the breeding period of the middle fish production step according to the present invention.
FIG. 11 is a graph showing the average weight change of each section during the breeding period of the middle fish production step according to the present invention.
FIG. 12 is a graph showing changes in feed efficiency of each section during the breeding period of the middle fish production step according to the present invention.
FIG. 13 is a graph showing the regression curve and the regression equation of the rapid rate of the eel fishes of the present invention.

The present invention aims at establishing a method of culturing Anguilla marmorata in a domestic farm in order to minimize the mortality of cultured fish and to produce high quality eels.

Foreign eel farming experiments consisted of an 'initial stocking stage' in which 108 eel of eels less than 0.15 g per day were raised to a maximum of 3 g per marriage, and an average of 3.6 g eels per day were raised for up to 33.6 g per marine And 'production stage of the Chinese language'.

I. Initial stocking stage

1. Preparation and methods of breeding

1.1 Preparation

A total of 6,534 g (44,127) of the test eels were purchased through transplantation approval of A. marmorata , a Southeast Asian eel collected in the Philippines. The test fishes were housed in 9 tanks in the same number of 726g (4,903) per dish. For the stabilization of the eel before storage, the stock was kept at 5 ‰ in Nacl during the initial stocking and gradually converted to complete fresh water ).

The experimental facility was used for groundwater rehydration, after warming from the storage tank to the test water temperature. Dissolved oxygen (DO) was maintained using a ring brush and liquefied oxygen. The rearing tank used a water tank of 1.0m X 1.0m X π (3.14m 2), and the depth of the rearing water was gradually increased from 0.2m to 0.7m (Fig. 1). The following is the status of stocking in the experiment tank.

Experimental tank 9

Number 1 tank: 726g (4,903), water temperature 26 ℃, rate of 100%

2 tanks: 726 g (4,903 tanks), water temperature 26 ° C, feed rate 75%

3 tanks: 726 g (4,903 tanks), water temperature 26 ° C, feed rate 50%

4 tanks: 726 g (4,903 tanks), water temperature 29 캜, feed rate 100%

No. 5 tanks: 726 g (4,903 tanks), water temperature 29 ° C, feed rate 75%

No. 6 tank: 726 g (4,903 grains), water temperature 29 ° C, feed rate 50%

7th water tank: 726g (4,903), water temperature 32 ℃, feed rate 100%

8th water tank: 726g (4,903), water temperature 32 ℃, feed rate 75%

9th water tank: 726g (4,903), water temperature 32 ℃, feed rate 50%

1.2. Breeding management

Breeding water was used after the ground water was heated to the test water temperature, and the number of breeding tank in the test section was completely returned twice a day, and dissolved oxygen was supplied by ring blower and liquefied oxygen. The rearing water temperature was divided into three sections (26 ℃, 29 ℃, 32 ℃) in order to find the optimum rearing temperature (Fig. 2). As shown in FIG. 2, in order to maintain the optimum temperature of the test water, a 2 kW automatic heater was installed in each test tank to maintain a proper water temperature, and a cold temperature sensor and water temperature automatic equipment The setting range of the breeding temperature was precisely maintained.

 During the test period, all water was returned to the feed water tank (100% twice a day) in the feeding water tank 2 hours later. The basal water quality was measured 2 hours after the feeding of dissolved oxygen, pH, ammonia, etc., and dissolved oxygen and pH were measured using the YSI550A and YSI60 products. The ammonia, (CHEMetrics) were used. During the test period, dissolved oxygen ranged from 3.6 to 8.8 mg / L, pH ranged from 7.8 to 8.6 and ammonia ranged from 0.0 to 1.7 mg / L.

1.3. How to pay

The foreign eel eel fish diet series was developed based on the Far Eastern eel fish diet series.

1) Thread eel mouth

The commercially available diets containing 20% protein and 1% fat were used as raw materials for the test. The main raw materials were shrimp and squid. The first mouth was completely melted and the juice was dispensed evenly throughout the breeding tank in a juice state so that the eels could learn the smell. The juice was switched from juice to sliced when the juice was adapted to a certain extent, and the juice blower stopped operating when the juice was fed (Fig. 3, B). The eel of the eel is white. After 5 ~ 7 days of feeding, the color of the eel becomes black due to the water temperature, and signs of feeding activity are visible.

2) Conversion of compound feed

60w incandescent lamps were lighted in the feeding area of the feeding tank to attract the eels to the feeding place. After the feeding of the mouthwash was stopped once, the compound feed conversion was carried out. Grapefruit feed and white rye feed are mixed in a kneader. Gradually, the feed amount is decreased and the amount of white rye feed is increased, and the water content is adjusted and supplied smoothly enough. Then, the conversion was induced by gradually adapting to the compound feed, and conversion to white porcelain, surplus, and chimpanzee was carried out according to the growth stages.

Eel fish diet by growth stage Classification Thread eel (white porcelain) for
(For dough) Surplus
(For dough) For chewing
(For dough) Cultivation
(For dough) Cultivation
(pellet) One 2 3 One 2 One 2 One 2 Fish meal 72 71 70 70 65 70 66 69 65 63 Casein Na 3 6 3 Skim milk powder 3 Starch powder 2 2 2 2 2 2 Active wheat gluten 10 6 8 3.8 5 3 2 5 Beer yeast 2 2 3 2.8 4.1 4.1 5.4 3 6 Soybean oil (50%) 4.4 α-potato starch 4.8 9.5 5.8 20 16 22 22 22 22 Wheat flour 22.4 Vitamin mixture 2 2 2 1.5 1.5 1.3 1.3 One One One Choline chloride (50%) 0.5 0.5 0.5 0.4 0.4 0.3 0.3 0.3 0.3 0.3 Inorganic compounding agent 2.3 2.3 2.3 2.3 2.3 2.3 2.3 2.3 2.3 2.3 Polyacrylic acid Na 0.2 0.3 0.2 0.2 Alginic acid Na 0.2 0.4 0.2 0.8 Guar gum One One

In order to determine the rate of eelgrass eelgrass, 50%, 75% and 100% of eelgrass eelgrass rate table (Fig. 4) was used. In the case that the remaining feed is excessively fed to the eel of the eels, the remaining feed may be decomposed in the water. In this case, even if the whole water is returned to the water, (Fig. 3, C).

In addition, the eel is a fish that has a vigorous food activity, and it is possible to prevent the growth rate from slowing down due to indigestion and so on by deciding the appropriate amount of feed. Therefore, it is important to establish an optimal feed rate for the growth characteristics of Southeast Asian eels.

Calculation of feeding rate according to the Eels rate chart is as follows. The regression equations for the rate of increase in the number of birds per kg per 100 kg of the eel were set to y = 2.078 ln (x) -5.925 (where x = number of eel / kg, y = percentage of feed quantity relative to eel weight).

Therefore, since 723 g of the Southeast Asian wild eel used in the present invention contains 4903 grains, when feeding at a rate of 100% at 6753 grains per kg, when feeding 12.4% of the total weight, 726 x 0.124 = 90.024 g is fed , 67.518g at 75% feed, and 45.012g at 50% feed rate. The feeding rate of the feed was measured by weighing 10 samples per day, and the average weight was measured.

The feed used for the test was commercial eel powder (more than 48% protein, more than 5% fat) and it was 100%, 75% and 50% : 00, 17: 00), and the amount of vitamin E, C, digestive agent, and other nutrients generally added to the feed at the feed rate was fed at a feed rate, ).

1.4. Test word analysis

In order to analyze the growth of test fish in each section, total weight and average body weight change and feed efficiency were investigated and the survival rate of each section was examined. Tests of each section The weight of the eels was measured and analyzed with a Kass electronic balance (error range ± 10g) held at our site.

2. Early Breeding Results

2.1 Thread Eel Growth

FIG. 5 is a graph showing changes in total weight of each section during the rearing period. The total weight started from the initial 726g with all sections equally structured. Water temperature 26 ℃ ℃ 11,545.8g in the feeding rate of 50%, 12,388.1g in the feeding rate 75%, 12157.6g in the feeding rate 100%, 8,980.9g in the feeding rate 50% and the feeding rate 75% The feed intake was increased from 13% to 13,642.2 g at 100% feed rate, and increased to 13,854.9 g at 50% feed rate, 14,526.6 g at 75% feed rate and 14,324.1 g at 100% feed rate at the water temperature of 32 ℃ .

The overall growth was good at 32 ℃ when the whole section was compared. The highest growth was observed from 726g to 14,526.6g before harvesting at the feed rate of 75%, and it was the highest at 32 ℃ and 8,980.9g at the feed rate of 50% at 29 ℃ And the lowest growth was observed.

FIG. 6 is a graph showing the average weight change of each section during the rearing period. During the test period, the average weight change of the eel began at 0.15 g. Water temperature was 26 ℃, and the growth rate was 2.64g at 50% of feeding rate, 2.64g at feeding rate of 75% and 2.62g at feeding rate of 100%, 1.87g at feed temperature of 50% And 2.83g in the feeding rate of 100%, and 2.90g in the feed rate of 50% of the feed temperature at 32 ℃, 3.03g in the feeding rate of 75%, and 2.95g in the feeding rate of 100%. When the whole section was compared, the average weight was also the highest in the temperature range of 32 ° C, and the highest growth was observed in the feeding rate of 75%.

The regression equation of the optimum feeding rate of Southeast Asian eel which corresponds to the feeding rate of 75% of the feeding rate of the Far Eastern eels is y = 1.5585 ln (x) -4.4434 (R ² = 0.9958) = Number of eels / kg, y = percentage value of feed quantity relative to eel weight) (Fig. 13).

2.2 Feed efficiency of Eel

Feed efficiency was calculated to compare the feeding rate and the degree of growth in the eel. Feed efficiency was calculated as the weight of dry feed required to increase the weight of the eels by one unit. For example, if 10 g of eels are fed with 100 g of feed and grown to 30 g, feed efficiency is 100 g / (30 g-10 g) = 5, and 50 g of dry feed is needed to grow 10 g of eel.

FIG. 7 is a graph showing changes in feed efficiency of each section during the rearing period. FIG. In the first (48th day) measurement, the highest value was 17.88% at the water temperature 32 ℃ feed rate 75%, and it was the lowest at 7.10% at the feed rate 100% at 26 ℃. In the second (day 77), the highest temperature was 77.58% at the temperature of 32 ℃ and the feed rate was 50%. In the third measurement (day 108), the highest feed rate of 50% was obtained at 32 ℃, and the lowest value was obtained at the feed rate of 100% at 32 ℃. In general, the feed rate was 50% at 32 ℃, and the feed efficiency was the highest at 50% feed rate.

Feed efficiency of Far Eastern eel culture is known to be about 66% (Eel culture standard guide, National Fisheries College 2009), and feed efficiency of Southeast Asia eel is lower than that of Far East. In this study, it was considered that feeds for commercial use of Far Eastern eels were applied because the feeds for Southeast Asian eels were not developed, and further study on the development of dietary feeds for Southeast Asia will be needed in the future.

As a result of this study, when the growth and feed efficiency of Southeast Asian eels were analyzed, the growth rate was the highest in the feed rate of 75% at the temperature of 32 ℃ for the growth, and the feed efficiency was 50% % Area was the best surveyed. However, there is no significant difference between the feeding rate of 75% and the growth rate of 50% at the water temperature of 32 ℃ in the breeding water temperature. Therefore, considering the economical aspect, the feeding rate of 50% is the best.

Ministry of Maritime Affairs and Fisheries According to the study on eel culture technology of European and North American eel in 2000, European and North America reported that the water temperature was 27 ° C and Far East acid was the best growth at 29 ° C. The water temperature was 32 ℃ and the fastest growth and feed efficiency were obtained at 32 ℃ in Southeast Asian eels.

2.3 Survival rate of the eel

8 is a graph showing the survival rate of each section during the breeding period. Our company generally suffered from dorsal erosion in the early days of the stocking. It is believed that this is due to the stress of transporting water from the Philippines to Korea, and there was no significant amount of mortality in each tank during the test.

The survival rate of each section was 94.51 ~ 98.96%, indicating a high overall survival rate. Compared with the Far Eastern eel, survival rate does not show any significant difference, so it is expected that the possibility of cultivation can be expected.

II. Cylinder production stage

1. Preparation and methods of breeding

1.1 Preparation

The test fish were purchased from the farms of Southeast Asian Anguilla marmorata and used for the test. At the start of the experiment, the average weight of the test fish was 3.6g, and 3.6kg (1,000 fish) were housed in each of nine water tanks in the same manner. The breeding aquarium was manufactured by making a small circulation filtration system tank. The test section status is as follows.

Experimental tank 9

No. 1 water tank: 3.6 kg (1,000 fish), water temperature 26 ℃, feed rate 100%

No. 2 tank: 3.6kg (1,000 fish), water temperature 26 ℃, feed rate 75%

3 tanks: 3.6 kg (1,000 tanks), water temperature 26 ℃, feed rate 50%

4 tanks: 3.6 kg (1,000 tanks), water temperature 29 ℃, rate of 100%

5 tanks: 3.6 kg (1,000 tanks), water temperature 29 캜, feed rate 75%

6 tanks: 3.6 kg (1,000 tanks), water temperature 29 ℃, feed rate 50%

No. 7 water tank: 3.6 kg (1,000 fish), water temperature 32 ° C, feed rate 100%

8 tanks: 3.6 kg (1,000 tanks), water temperature 32 ° C, feed rate 75%

9th water tank: 3.6kg (1,000 fish), water temperature 32 ℃, feed rate 50%

1.2. Breeding management

 The water was used after the ground water was warmed to the test water temperature and the experimental study was divided into three sections (26 ℃, 29 ℃, 32 ℃) to find the positive positive water temperature of Southeast Asian eel. The circulation filtration method, which is a method of rehabilitation through physiological and biological filtration, was applied without discarding the breeding water, and the experiment was carried out by changing 5% of the total volume of the water in a day. The basic water quality survey was conducted in the same way as I.1.2 above. During the test period, dissolved oxygen was 6.9 ~ 8.3㎎ / L, pH was 6.9 ~ 7.8 and ammonia was 0 ~ 1 mg / L. Feeding water was returned 5% of the total amount 2 hours after the feeding of afternoon food. 3.6 ~ 8.8

1.3. How to pay

The feed used for the test was commercial eel powder (protein 46% or more, fat 3% or more). The test fish feed rate was established on the basis of the standard feed rate of Far East eels as shown in Fig. The amount of feed was calculated by the same method as in A.1.3 above, and the same interval as the Far East eel standard rate was set as the feeding rate of 100%. The other two settings were 75% of the Far Eastern eel standard rate 50% level, and 3 sections were set up twice a day (05:00, 17:00). The feeding rate of the feed was measured by weighing 10 samples per day, and the average weight was measured.

1.4. Test rod weight analysis

In order to analyze the growth of test fish in each section, total weight and average weight change and feed efficiency were examined and the survival rate of each section was examined (FIG. 9).

2. Breeding Experiment Results

2.1 Thread Eel Growth

FIG. 10 is a graph showing changes in the total weight of each section during the breeding period of the middle fish production step according to the present invention. The total weight was initially set at 3,600 g, with all sections being identically constructed. The temperature of 26 ℃ was increased from 13% to 13,632g in the feed rate of 50%, 15,594g in the feeding rate of 75% and to 22,839g in the feeding rate of 100%. In the water temperature of 29 ℃, the feeding rate was 15,354g in the feeding rate of 50% The feed rate increased from 22% to 20,530g at the feed rate of 50%, to 30,219g at the feed rate of 75% and to 29,521g at the feeding rate of 100% at the water temperature of 32 ° C . The overall growth was good at 32 ℃ when the whole section was compared. The highest growth rate was observed from the initial weight of 3,600 g to 30,219 g at the feeding rate of 75%. The highest growth was observed at 26 ℃ 50% And the lowest growth rate.

FIG. 11 is a graph showing the average weight change of each section during the breeding period of the middle fish production step according to the present invention. During the test period, the mean weight change of the test fish began at the initial 3.6 g. The water temperature was 26 ℃, and the growth rate was 14.7g at 50% feed rate, 16.6g at feed feed rate 75%, and 25.3g at 100% feed rate. 24.2g in the feeding interval, and 26.1g in the feeding rate of 100%. The growth rate was 25.0g at the temperature of 32 ℃ feeding feed 50%, 33.6g at the feeding rate 75%, and 32.4g at the feeding rate 100%. The average weight was also the highest in water temperature 32 ℃, and the highest growth rate was observed in 75% feeding.

The regression equation of the optimum feed rate of Southeast Asian eels, which corresponds to 75% of the Far Eastern eel rate, is y = 1.5585 ln (x) -4.4434 (R ² = 0.9958), where x = eel Weight (number of grains / kg), y = percentage of feed quantity relative to eel weight), thereby establishing the optimum feed rate reference table for Southeast Asian eels.

2.2 Feed efficiency of Eel

FIG. 12 is a graph showing changes in feed efficiency of each section during the breeding period of the middle fish production step according to the present invention. In the first (30th day) measurement, the highest temperature was 47.11% at the water temperature of 32 ℃ and the feed rate of 50%, and the lowest value was 17.39% at the feed rate of 100% at 26 ℃. On the second day (60th day), the highest temperature was 50.95% in the temperature range of 32 ℃ and 50% of the feed rate, and the lowest value was 22.4% in the temperature range of 26 ℃ and 75%. The regression equation of feeding rate of 50% is equal to y = 1.038 ln (x) -2.953. Where y represents the percentage of feed quantity relative to eel weight and x represents eel weight (number of mules / kg).

After that, the feed efficiency tended to increase gradually in the whole period. At the 150th day of the measurement, the temperature of 32 ℃ feed was 50%, which was the highest at 74.54%, and the lowest value was 41.64% at the feed rate of 100 ℃ at 26 ℃. During the test period, water temperature was 32 ℃ and feed rate was 50%, which showed the highest feed efficiency and lowest value at 26 ℃. Therefore, it was confirmed that intestinal eel growth and feed efficiency were high at 32 ℃, which is the high water temperature in the test section.

2.3 Survival rate of the eel

During the breeding period, our company seems to have been caused by stress and trauma due to transportation, stocking, new environment adaptation, etc. Thereafter, no adaptation to the test tank was found, and no deaths due to special symptoms were found in each section. The final survival rate was 89 ~ 94%.

III. Review

Koreans tend to prefer natural products rather than farm animals, and domestic eels ( A. eaponica ) rather than foreign eels. Eels are popular with the public as a favorite health food, but they show a sharp decline due to environmental pollution, the formation of dike banks, and ecosystem changes. In this regard, Southeast Asian eels were tested and cultured as a species to search for stagnant eel culture with the development of aquaculture technology of new eel fish species that have not been developed yet.

Southeast Asian eels ( Anguilla. Mossambica ) are fish species that live in Korea with Far Eastern eels, so they can help increase farmers' income if their emotional resistance is low and the farming methods are well established. As a result, the weight gain and feed efficiency of Southeast Asian eel were higher than that of Far Eastern eel at 32 ℃ and the feed rate of southeastern eel and the feed rate regression equation y = 1.5585ln (x) -4.4434 (R ² = 0.9958), where x = number of eelgrass / kg, y = percentage of feed quantity relative to eel weight.

The growth rate was slower than that based on the above rate-of-pay table, but mass production was not visible as in the case of general price. Growth weight and feed efficiency are lower than that of Far East eels, so the rearing period may be somewhat longer. However, it is expected that many benefits will be expected if the eel farming price is low (one tenth of Far Eastern mountain) do.

As a result of carrying out a positive test study for 108 days in the initial stocking stage, the water temperature showed the highest growth at 32 ℃ in 26, 29, and 32 ℃, and the average eel weight was 3g As a result, the highest growth rate was observed at the highest water temperature of 32 ℃.

The feed rate showed the highest growth rate at the feed rate of 100% feed rate at 26 ℃ and 29 ℃, but the feed rate was almost the same at 75% feed rate and 100% feed rate at 32 ℃. However, if feed efficiency is considered, the feeding rate will be the best at 75%.

The final survival rate was 89 ~ 94%, which showed a stable survival rate as in the initial stage of western stage. Therefore, in the case of culturing southeastern eels by the culturing method disclosed in the present invention, it is expected that the eel cost will be low and the supply and demand will be stabilized.

The invention foreign eel (Anguilla marmorata ) method, it is possible to reduce the mortality rate of foreign eel which has been killed easily by raising the optimum amount of feed by feeding at the optimal temperature of the rearing water condition and by establishing the domestic rearing method, It is possible to substitute for the unstable Far Eastern mountain eel, and it is possible to cultivate the Yangyang industry stably and contribute to increase the income of farmers.

Claims (3)

Anguilla In the cultivation method of marmorata ,
a) For the stabilization of the eels before accepting the eel, keeping the nursing water at the time of the initial stocking is maintained at 5 ‰;
b) After the adaptation of step a), the step of mixing and feeding the granular feed and the white flour feed;
c) After the step b), the step of culturing the Anguilla marmorata is carried out by feeding the feed in the order of white porcelain, black bean, and tiger chow.
The method of claim 1, wherein the step a) to c) can be bred in phase temperature to 32 ℃ and the feed amount to be administered 1.5585ln = y (x) Anguilla characterized in that the star with the regression equation of -4.4434 How to make marmorata .
Where y represents the percentage of feed quantity relative to the eel weight and x represents the eel weight (mug / kg).
The method of claim 1, wherein the step a) to c) can be bred in phase temperature to 32 ℃ and the feed amount to be administered 1.038ln = y (x) Anguilla marmorata characterized in that the grade of the regression equation -2.953 The method of cultivation.
Where y represents the percentage of feed quantity relative to the eel weight and x represents the eel weight (mug / kg).

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