CN103548735A - Grouper fry propagation method - Google Patents
Grouper fry propagation method Download PDFInfo
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- CN103548735A CN103548735A CN201310527708.8A CN201310527708A CN103548735A CN 103548735 A CN103548735 A CN 103548735A CN 201310527708 A CN201310527708 A CN 201310527708A CN 103548735 A CN103548735 A CN 103548735A
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- 241001417495 Serranidae Species 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 24
- 241000251468 Actinopterygii Species 0.000 claims abstract description 32
- 230000001105 regulatory effect Effects 0.000 claims abstract description 13
- 230000000366 juvenile effect Effects 0.000 claims abstract description 9
- 230000000091 immunopotentiator Effects 0.000 claims abstract description 8
- 238000011534 incubation Methods 0.000 claims abstract description 8
- 230000001276 controlling effect Effects 0.000 claims abstract description 6
- 150000001875 compounds Chemical class 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 239000000843 powder Substances 0.000 claims description 15
- 235000013312 flour Nutrition 0.000 claims description 10
- 230000037396 body weight Effects 0.000 claims description 9
- 230000003203 everyday effect Effects 0.000 claims description 9
- 239000013535 sea water Substances 0.000 claims description 9
- 241000238557 Decapoda Species 0.000 claims description 8
- 241000411851 herbal medicine Species 0.000 claims description 7
- 241000239250 Copepoda Species 0.000 claims description 6
- 241000700141 Rotifera Species 0.000 claims description 6
- 238000005273 aeration Methods 0.000 claims description 6
- 235000013305 food Nutrition 0.000 claims description 6
- 235000016709 nutrition Nutrition 0.000 claims description 6
- 230000035764 nutrition Effects 0.000 claims description 6
- 238000005728 strengthening Methods 0.000 claims description 6
- 240000002234 Allium sativum Species 0.000 claims description 5
- 241000205585 Aquilegia canadensis Species 0.000 claims description 5
- 241000576429 Forsythia suspensa Species 0.000 claims description 5
- 241000361919 Metaphire sieboldi Species 0.000 claims description 5
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 5
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 5
- 240000001341 Reynoutria japonica Species 0.000 claims description 5
- 235000018167 Reynoutria japonica Nutrition 0.000 claims description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 5
- 241000254109 Tenebrio molitor Species 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 239000010231 banlangen Substances 0.000 claims description 5
- YBHILYKTIRIUTE-UHFFFAOYSA-N berberine Chemical compound C1=C2CC[N+]3=CC4=C(OC)C(OC)=CC=C4C=C3C2=CC2=C1OCO2 YBHILYKTIRIUTE-UHFFFAOYSA-N 0.000 claims description 5
- 229940093265 berberine Drugs 0.000 claims description 5
- QISXPYZVZJBNDM-UHFFFAOYSA-N berberine Natural products COc1ccc2C=C3N(Cc2c1OC)C=Cc4cc5OCOc5cc34 QISXPYZVZJBNDM-UHFFFAOYSA-N 0.000 claims description 5
- 239000002775 capsule Substances 0.000 claims description 5
- 239000003026 cod liver oil Substances 0.000 claims description 5
- 235000012716 cod liver oil Nutrition 0.000 claims description 5
- 230000003750 conditioning effect Effects 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- 235000004611 garlic Nutrition 0.000 claims description 5
- 241000257465 Echinoidea Species 0.000 claims description 3
- 241000133262 Nauplius Species 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 230000002354 daily effect Effects 0.000 claims description 3
- 229910052736 halogen Inorganic materials 0.000 claims description 3
- 150000002367 halogens Chemical class 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 235000015170 shellfish Nutrition 0.000 claims description 3
- 230000000638 stimulation Effects 0.000 claims description 3
- 238000013022 venting Methods 0.000 claims description 3
- 239000003643 water by type Substances 0.000 claims description 3
- 230000008569 process Effects 0.000 abstract description 3
- 230000004083 survival effect Effects 0.000 abstract description 3
- 238000013461 design Methods 0.000 abstract description 2
- 201000010099 disease Diseases 0.000 abstract description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 2
- 230000036737 immune function Effects 0.000 abstract description 2
- 210000000582 semen Anatomy 0.000 abstract description 2
- 235000013601 eggs Nutrition 0.000 abstract 4
- 238000009395 breeding Methods 0.000 abstract 1
- 230000001488 breeding effect Effects 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 230000026109 gonad development Effects 0.000 abstract 1
- 241000287828 Gallus gallus Species 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000000384 rearing effect Effects 0.000 description 2
- 241000489963 Anabantoidei Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000357439 Epinephelus Species 0.000 description 1
- 241000276618 Perciformes Species 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000002710 gonadal effect Effects 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 238000009364 mariculture Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 235000014102 seafood Nutrition 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Fodder In General (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a grouper fry propagation method, which belongs to the technical field of marine culture. The grouper fry propagation method comprises the following steps: (1) selecting parent fish; (2) breeding the parent fish; (3) collecting fertilized ova; (4) manually regulating and controlling the incubation of the fertilized ova; (5) manually regulating and controlling the breed of fry, advanced fry and juvenile fish. The method is rational in design and has strong operability; by adding Vc, VE and compound traditional Chinese medicine immunopotentiator in a feed fed in a parent fish culture process, the potential disease in the bodies of the parent fish can be prevented, the immunologic functions of the bodies of the parent fish are enhanced, and mature gonad development of the parent fish is promoted, so that the fish naturally lay eggs, and meanwhile, the quality of the eggs and the semen is improved; by manually regulating and controlling the incubation of the fertilized eggs and the culture of the fry, the advanced fry and the juvenile fish, stable and large-scale fry are formed. According to the method, the fertilized eggs reach over 97 percent, the incubation rate reaches over 90 percent, and the survival rate reaches over 2.1 percent.
Description
Technical field
The invention belongs to mariculture technology field, be specifically related to grouper seed expanding propagation method.
background technology
Grouper
epinephelubelong to Perciformes, Anabantoidei ，Lv section, Epinephelus.In subtropics, the whole world and Tropical Ocean Area, all having distribution, is important marine fish.Grouper has that fast growth, feed coefficient are low, economic worth advantages of higher, is high protein, low-fat first-class edible fishes, is considered to have most under intensive culture condition the fish of cultivation prospect.Grouper is nutritious, and fine and tender taste is pure white, and similar chicken have the title of " extra large chicken ", is well received by consumers.Grouper is also one of seafood fish of artificial propagation and seedling growing process difficulty maximum simultaneously, and at present due to the required grouper seedling famine of cultivation, the grouper mass rearing of research and development scale is necessary very much.
Summary of the invention
For problems of the prior art, the object of the invention is providing a kind of grouper seed expanding propagation method.
The present invention is realized by the following technical programs:
Described grouper seed expanding propagation method, is characterized in that comprising following steps:
1) selection of parent population
The fish body of catching from natural waters, select physique stalwartness, reaction quick, without deformity, body surface is intact the wounded as parent population, raun body weight 600-800g/ tail wherein, milter body weight is more than or equal to 1800g/ tail, male and female parent population is arranged in pairs or groups by the ratio of 1:2;
2) cultivation of parent population
The good grouper parent population of combo is supported temporarily in indoor culture pond, parent population is carried out to prenatal nutrition strengthening and environment conditioning, impel its natural spawning, obtain fertilized egg;
Described prenatal nutrition strengthening, is to take fresh small fish as bait, adds the Vc, the 1-2%V that account for bait weight 2-3%
e,3-4% seawater compound Chinese herbal medicine immunopotentiator for fish, throws something and feeds twice every day, and a day feeding volume is the 3.5-4.5% of parent population body weight; Described parent population environment conditioning refers to cultivates 25 ℃～26 ℃ of pond water temperatures, seawater salinity 25～35, and PH8.0～8.3, cultivating pond water surface intensity of illumination is 3500-4500Lx, grouper is carried out the stimulation by running water 20～30 minutes of 43-48 liter/min simultaneously;
3) collect fertilized egg
Utilize the egg collecting groove of cultivating in pond to collect fertilized egg;
4) artificial regulatory incubating oosperm
Fertilized egg is positioned over and is cultivated in pond, and controlling and cultivating pond water temperature is 25.5～26 ℃, and seawater salinity 28～32, hatches under pH value 8.1～8.2, micro-venting condition, and water-soluble oxygen amount is more than or equal to 6mg/L;
5) young, young, the juvenile fish of artificial regulatory is cultivated
Described artificial regulatory is young, young, juvenile fish is cultivated, and refers to 25～26 ℃ of water temperatures, salinity 29～31, and pH value 8.1～8.2, aeration quantity is changed from small to big, and a day quantity of exchanged water is 27%-32% alevin stage, postlarva phase 55%-60%, young stage 80%-90%;
2-3 after seedling incubation of membrane days starts the open-mouthed bait of throwing something and feeding, and open-mouthed bait is that shellfish larvae and sea urchin egg mix in the ratio of 1:1, evenly splashes, and throws something and feeds every day four times, and each feeding volume is 8-12g/m
3, 2-3 days throws something and feeds;
From the 4th day of the bait of throwing something and feeding, start to throw something and feed wheel animalcule and copepoda nauplius were thrown something and fed in the ratio of 4:1, and it is 12000-18000/L that daily ration, feeding quantity be take the density that maintains wheel animalcule in water body, the two weeks of throwing something and feeding;
The large halogen worm of throwing something and feeding copepoda and living after fry 20 ages in days, feeding volume maintains 1500～1800/L with overall food density;
When fry grows to after 4～5 ㎝, start to throw something and feed shrimp and manufactured feed, its both ratios are 5:3, throw something and feed every day 2 times, feeding volume is that the concentrated food of robbing of fry stops.
Described grouper seed expanding propagation method, is characterized in that step 2) described in Chinese herbal medicine immunopotentiator by berberine: honeysuckle: the capsule of weeping forsythia: Radix Isatidis: garlic: the fleece-flower root: Radix Glycyrrhizae is that 2～3:1～2:0.5～1:0.4～0.8:0.5～1:0.2～0.5:0.3～0.8 is mixed to get.
Described grouper seed expanding propagation method, is characterized in that in step 4), micro-inflation refers at most at 12-15L/m per hour
3.
Described grouper seed expanding propagation method, is characterized in that in step 5) that aeration quantity is changed from small to big to refer to 15L/m per hour
3increase to gradually 45L/m per hour
3.
Described grouper seed expanding propagation method, is characterized in that the manufactured feed described in step 5) is by dregs of beans: corn flour: flour: dusty yeast: cod-liver oil: shrimp head powder: shitosan: earthworm powder: yellow mealworm powder is that 4～5:2～3:1～2:0.5～0.8:3～4:1.5～3.5:1～2:0.5～1.5:0.5～1.5 are mixed and made.
The inventive method is reasonable in design, workable, by add Vc, V in the bait of throwing something and feeding in parent fish rearing process
eand Chinese herbal medicine immunopotentiator, can prevent potential disease in parent population fish body, strengthen the immunologic function of parent population fish body, impel parent population gonadal maturation, make it natural spawning, improve the quality of ovum grain and seminal fluid simultaneously; By artificial regulatory incubating oosperm and son, young, juvenile fish, cultivate, form seed stable, in batches, by the inventive method, fertilized egg reaches more than 97%, and incubation rate reaches more than 90%, and survival rate reaches more than 2.1%.
Embodiment
By following specific embodiment, the invention will be further described.
Embodiment
1) selection of parent population
The fish body of catching from natural waters, select physique stalwartness, reaction quick, without deformity, body surface is intact the wounded as parent population, raun body weight 600-800g/ tail wherein, milter body weight is 1800g/ tail at least, male and female parent population is arranged in pairs or groups by the ratio of 1:2;
2) cultivation of parent population
The good grouper parent population of combo is supported temporarily in indoor culture pond, control and cultivate 25 ℃ of pond water temperatures, 25.5 ℃ or 26 ℃, control seawater salinity 25, 30 or 35, control pH value 8.0, 8.1 or 8.3, control and cultivate pond water surface intensity of illumination 3500 Lx, 4000 Lx or 4500 Lx, grouper is carried out to 43 simultaneously, the stimulation by running water 20 of 45 or 48 liters/min, 25 or 30 minutes, during supporting temporarily, twice carry out prenatal nutrition strengthening to the parent population bait of throwing something and feeding every day, day feeding volume is 3.5% of parent population body weight, 4% or 4.5%, bait is fresh small fish, add the Vc that accounts for bait weight 2-3% simultaneously, 1-2%V
e,3-4% seawater compound Chinese herbal medicine immunopotentiator for fish, this Chinese herbal medicine immunopotentiator is by berberine: honeysuckle: the capsule of weeping forsythia: Radix Isatidis: garlic: the fleece-flower root: Radix Glycyrrhizae is that 2:1.5:0.5:0.4:1:0.5:0.3 is mixed to get, or by berberine: honeysuckle: the capsule of weeping forsythia: Radix Isatidis: garlic: the fleece-flower root: Radix Glycyrrhizae is that 2.5:1:0.8:0.8:0.5:0.2:0.8 is mixed to get, or by berberine: honeysuckle: the capsule of weeping forsythia: Radix Isatidis: garlic: the fleece-flower root: Radix Glycyrrhizae is that 3:2:1:0.6:0.7:0.3:0.5 is mixed to get, adopt the method to carry out prenatal nutrition strengthening and environment conditioning to parent population, impel natural spawning, obtain fertilized egg,
3) collect fertilized egg
Utilize the egg collecting groove of cultivating in pond to collect fertilized egg;
4) artificial regulatory incubating oosperm
Fertilized egg is positioned over and is cultivated in pond, and controlling and cultivating pond water temperature is 25.5,25.8 or 26 ℃, seawater salinity 28,30 or 32, and pH value 8.1 or 8.2, is 12 L/m per hour in aeration quantity
3, 13.5 L/m
3or 15L/m
3micro-venting condition under hatch, water-soluble oxygen amount is more than or equal to 6mg/L;
5) young, young, the juvenile fish of artificial regulatory is cultivated
Artificial regulatory is young, young, juvenile fish is cultivated, and control and cultivate 25,25.5 or 26 ℃ of pond water temperatures, salinity 29,30 or 31, pH value 8.1 or 8.2, aeration quantity is 15L/m per hour
3increase to gradually 45L/m per hour
3, a day quantity of exchanged water is alevin stage 27%, 30% or 32%, postlarva phase 55%, 58% or 60%, young stage 80%, 85% or 90%; After seedling seedling incubation of membrane, within 2-3 days, start the open-mouthed bait of throwing something and feeding, open-mouthed bait is that shellfish larvae and sea urchin egg mix in the ratio of 1:1, evenly splashes, and throws something and feeds every day four times, and each feeding volume is respectively 12g/m
3, 10g/m
3, 8g/m
3, 9g/m
3, throw something and feed two days or three days; From the 4th day of the bait of throwing something and feeding, start to throw something and feed wheel animalcule and copepoda nauplius were thrown something and fed in the ratio of 4:1, and it is 12000-18000/L that daily ration, feeding quantity be take the density that maintains wheel animalcule in water body, the two weeks of throwing something and feeding; The large halogen worm of throwing something and feeding copepoda and living after fry 20 ages in days, feeding volume maintains 1500～1800/L with overall food density; When fry grows to after 4～5 ㎝, start to throw something and feed shrimp and manufactured feed, its both ratios are 5:3, throw something and feed every day 2 times, feeding volume is that the concentrated food of robbing of fry stops.This manufactured feed is by dregs of beans: corn flour: flour: dusty yeast: cod-liver oil: shrimp head powder: shitosan: earthworm powder: yellow mealworm powder is that 4:3:1:0.8:3:2.5:1.5:1:0.5 mixes and makes, or by dregs of beans: corn flour: flour: dusty yeast: cod-liver oil: shrimp head powder: shitosan: earthworm powder: yellow mealworm powder is that 4.5:2.5:1.5:0.5:4:3.5:2:1.5:1 mixes and makes, or by dregs of beans: corn flour: flour: dusty yeast: cod-liver oil: shrimp head powder: shitosan: earthworm powder: yellow mealworm powder is that 5:2:2:0.7:3.5:1.5:1:0.5:1.5 mixing makes.
Within 2012, adopt the concrete grammar of this invention to 1500 of female groupers, 3000 of male groupers are cultivated, mating, reproduction test, and last fertilized egg reaches more than 97%, and incubation rate reaches more than 90%, and survival rate reaches more than 2.1%.
Claims (5)
1. grouper seed expanding propagation method, is characterized in that comprising following steps:
1) selection of parent population
The fish body of catching from natural waters, select physique stalwartness, reaction quick, without deformity, body surface is intact the wounded as parent population, raun body weight 600-800g/ tail wherein, milter body weight is more than or equal to 1800g/ tail, male and female parent population is arranged in pairs or groups by the ratio of 1:2;
2) cultivation of parent population
The good grouper parent population of combo is supported temporarily in indoor culture pond, parent population is carried out to prenatal nutrition strengthening and environment conditioning, impel its natural spawning, obtain fertilized egg;
Described prenatal nutrition strengthening, is to take fresh small fish as bait, adds the Vc, the 1-2%V that account for bait weight 2-3%
e,3-4% seawater compound Chinese herbal medicine immunopotentiator for fish, throws something and feeds twice every day, and a day feeding volume is the 3.5-4.5% of parent population body weight; Described parent population environment conditioning refers to cultivates 25 ℃～26 ℃ of pond water temperatures, seawater salinity 25～35, and PH8.0～8.3, cultivating pond water surface intensity of illumination is 3500-4500Lx, grouper is carried out the stimulation by running water 20～30 minutes of 43-48 liter/min simultaneously;
3) collect fertilized egg
Utilize the egg collecting groove of cultivating in pond to collect fertilized egg;
4) artificial regulatory incubating oosperm
Fertilized egg is positioned over and is cultivated in pond, and controlling and cultivating pond water temperature is 25.5～26 ℃, and seawater salinity 28～32, hatches under pH value 8.1～8.2, micro-venting condition, and water-soluble oxygen amount is more than or equal to 6mg/L;
5) young, young, the juvenile fish of artificial regulatory is cultivated
Described artificial regulatory is young, young, juvenile fish is cultivated, and refers to 25～26 ℃ of water temperatures, salinity 29～31, and pH value 8.1～8.2, aeration quantity is changed from small to big, and a day quantity of exchanged water is 27%-32% alevin stage, postlarva phase 55%-60%, young stage 80%-90%;
2-3 after seedling incubation of membrane days starts the open-mouthed bait of throwing something and feeding, and open-mouthed bait is that shellfish larvae and sea urchin egg mix in the ratio of 1:1, evenly splashes, and throws something and feeds every day four times, and each feeding volume is 8-12g/m
3, 2-3 days throws something and feeds;
From the 4th day of the bait of throwing something and feeding, start to throw something and feed wheel animalcule and copepoda nauplius were thrown something and fed in the ratio of 4:1, and it is 12000-18000/L that daily ration, feeding quantity be take the density that maintains wheel animalcule in water body, the two weeks of throwing something and feeding;
The large halogen worm of throwing something and feeding copepoda and living after fry 20 ages in days, feeding volume maintains 1500～1800/L with overall food density;
When fry grows to after 4～5 ㎝, start to throw something and feed shrimp and manufactured feed, its both ratios are 5:3, throw something and feed every day 2 times, feeding volume is that the concentrated food of robbing of fry stops.
2. grouper seed expanding propagation method according to claim 1, is characterized in that step 2) described in Chinese herbal medicine immunopotentiator by berberine: honeysuckle: the capsule of weeping forsythia: Radix Isatidis: garlic: the fleece-flower root: Radix Glycyrrhizae is that 2～3:1～2:0.5～1:0.4～0.8:0.5～1:0.2～0.5:0.3～0.8 is mixed to get.
3. grouper seed expanding propagation method according to claim 1, is characterized in that in step 4), micro-inflation refers at most at 12-15L/m per hour
3.
4. grouper seed expanding propagation method according to claim 1, is characterized in that in step 5) that aeration quantity is changed from small to big to refer to 15L/m per hour
3increase to gradually 45L/m per hour
3.
5. grouper seed expanding propagation method according to claim 1, is characterized in that the manufactured feed described in step 5) is by dregs of beans: corn flour: flour: dusty yeast: cod-liver oil: shrimp head powder: shitosan: earthworm powder: yellow mealworm powder is that 4～5:2～3:1～2:0.5～0.8:3～4:1.5～3.5:1～2:0.5～1.5:0.5～1.5 are mixed and made.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104905033A (en) * | 2015-05-22 | 2015-09-16 | 珠海海龙生物科技有限公司 | Compound feed for micropterus salmoides fry |
| CN105409855A (en) * | 2015-12-16 | 2016-03-23 | 宜章洪成特种水产繁育场 | Fry culturing method |
| CN105746399A (en) * | 2016-03-07 | 2016-07-13 | 五河县金满塘生态种养殖家庭农场 | Crucian breeding quality improvement method |
| CN110402857A (en) * | 2019-08-30 | 2019-11-05 | 三亚福联水产发展有限公司 | A kind of grouper quick-breeding method |
| CN111134055A (en) * | 2020-02-26 | 2020-05-12 | 海南晨海水产有限公司 | Efficient outdoor ecological pond artificial breeding method for epinephelus lanceolatus |
| CN111149738A (en) * | 2020-02-27 | 2020-05-15 | 海南晨海水产有限公司 | Efficient outdoor ecological pond artificial breeding method for hybrid seeds of Epinephelus coioides and Epinephelus hybridus |
| CN111183931A (en) * | 2020-02-25 | 2020-05-22 | 海南晨海水产有限公司 | Efficient outdoor ecological pond artificial breeding method for grouper |
| CN111194706A (en) * | 2020-02-26 | 2020-05-26 | 海南晨海水产有限公司 | Efficient artificial breeding method for Lutjanus argentimaculatus in outdoor ecological pond |
| CN111194707A (en) * | 2020-02-27 | 2020-05-26 | 海南晨海水产有限公司 | Efficient outdoor ecological pond artificial breeding method for red sea perch |
| CN111771772A (en) * | 2020-07-23 | 2020-10-16 | 福建省水产研究所（福建水产病害防治中心） | Grouper fry breeding method |
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