CN104170777A - Breeding method for amphiprion ocellarises - Google Patents
Breeding method for amphiprion ocellarises Download PDFInfo
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- CN104170777A CN104170777A CN201410382104.3A CN201410382104A CN104170777A CN 104170777 A CN104170777 A CN 104170777A CN 201410382104 A CN201410382104 A CN 201410382104A CN 104170777 A CN104170777 A CN 104170777A
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Abstract
The invention provides a breeding method for amphiprion ocellarises. The breeding method comprises the following three steps: a. breeding parent fishes; b. incubating oosperms; c. breeding young juvenile fish. Fry is bred for 60 d after being incubated, the overall length of the juvenile fish is up to 2 cm to 3 cm, and the breeding process is ended up; during the breeding process, the minced-meat-shaped fresh bait made of rotifer intensified by seawater chlorella for 24 h, artemia and copepods intensified by DHA and the chlorella for 12 h, and marine small animals of which phospholipid content is more than 25% in succession and compound feed are fed as the bait in an individual manner or a superimposed manner; furthermore, water is changed and the amphiprion ocellarises are divided and put in fish tanks in proper time. The breeding method for the amphiprion ocellaris, provided by the invention, provides a way which can be operated in an industrialized and large-scale manner; compared with the prior art, as the breeding method provided by the invention is of popularity to a higher degree, a foundation is provided for large-scale breeding of the amphiprion ocellaris.
Description
Technical field
What the present invention relates to is the seedling-cultivating method of a kind of son clown fish.
Background technology
Son clown fish (
amphiprion ocellaris) be subordinate to Perciformes (
perciformes), Pomacentridae (
pomacentridae), two saw fishes genus (
amphiprion).Its body is long, and generally at 5-8cm, maximum can reach 10cm, and build is oval, body colour Exocarpium Citri Rubrum, there are three white endless belt on side, lays respectively at after eyes, dorsal fin central authorities, caudal peduncle place, wherein the leukorrhea of dorsal fin central authorities forms triangle on side, each fin Chinese red, and some individualities have black border.Son clown fish life hierarchy is strict, sexual conversion behavior, and after sexual maturity, raun build is greater than milter.Mainly be distributed in South China Sea, Philippine and coral reef marine site, Western Pacific.
Son clown fish color beauty, has a sweet temper, healthy and strong active easily raise, and can with sea anemone symbiosis, be vast Shui nationality fan's first-selected kind.Owing to being subject to propagation technique and production-scale limitation, the son clown fish of selling is in the market still to fish for wild natural population as main.And in the time fishing for, the toxic chemical such as multiplex cyanide is fished for, and causes wild son clown fish lethality high, is difficult to raise and train.Meanwhile, cyanide also can cause damage to marine organisms such as corals, destroys the ecological balance of Coral Reef Region, and therefore the artificial breeding of seawater pet fish is imperative.Physiological ecological, the breeding of existing scholar to son clown fish both at home and abroad at present grown and done Primary Study.
Yang Chen, Li Hongwu have introduced the more detailed step of growing seedlings in " son clown artificial propagation and the Primary Study of growing seedlings " literary composition.
A. parent fish rearing: " selective body length exceedes the conduct female parent of 7cm, manually matching for male parent below the long 6cm of body.Select altogether 18 pairs of parent populations, raise respectively in the glass aquarium of 18 90 × 60 × 60cm, in each aquarium, put tile as fish nest.
Water temperature maintains 28 ° of C, and dissolved oxygen is higher than 5.8 mg/L, and ammonia-nitrogen content is lower than 0.1 mg/L, and salinity is 30 ~ 32.Above water tank, throw light on 36 w fluorescent tubes in 20 cm places, illumination every day 14 h.Throw something and feed every day and break into rotten fresh shrimp and laver, throw something and feed 3 times respectively at 9:00,12:00 and 17:00.”
B. incubating oosperm: " fish-egg ... become silver-shiny ... sunset that afternoon before by adhere to fish-egg fish nest take out, transfer to hatching cylinder in.Hatching cylinder adopts the water in parent population cylinder, agitates the action of current by inflating simulation parent population, ovum group is slowly swung back and forth, with oxygen deficit proof.Then will hatch cylinder shading, prelarva can hatch in the environment of complete darkness, and hatching process continues 30 minutes conventionally.After 30-60 minutes, by the hatching situation of microvision prelarva, as complete in basic hatching, can remove shade, dropwise add chlorella algae liquid, and add the wheel animalcule through chlorella strengthening, density is 5-10/liters.”
C, larvae juvenile fish are cultivated: " second day, the prelarva of sucking-off death and cylinder bottom impurity, supplemented algae liquid and wheel animalcule.Repeatedly throw something and feed every day on a small quantity, ensures the density of wheel animalcule.Throw something and feed wheel animalcule two days later, a small amount of shrimps nauplius that starts to throw something and feed, and along with growing of prelarva increases the ratio that shrimps is thrown something and fed gradually.At the initial stage of growing seedlings, ensure to have in water body the chlorella of sufficient density, take out the end every day, supplement algae liquid.The be satiated with food obvious bulging of prelarva belly of wheel animalcule, presents silver color.
Through the growth of 5 days, grow the build of prelarva faster and start to become ellipse from taper, the shrimps nauplius of now throwing something and feeding in a large number, the postlarva belly of being satiated with food presents orange red.Well-developed postlarva can grow at 8-14 days head Article 1 leukorrhea, now increases throw something and feed copepoda and a small amount of powder feed.14-21 days, postlarva health middle part striped started to form, and the Powdered feed of now throwing something and feeding reduces the input of live bait gradually.At this moment postlarva is transferred in the large aquarium with the filtration improved and fed, make it have larger growing space.21-48 days, young fish length can reach 1cm left and right, and the black surround of pectoral fin starts to grow, the powder feed of now throwing something and feeding completely.48-64 days, clown fish grew into the long 1.5cm of body left and right, started to form striped and the black surround of afterbody.Now throw something and feed crush pellet.Through the growth of 64-90 days, postlarva grew to the long 2.5cm of body left and right, and black surround continues to deepen, appearance and parent population are as good as, juvenile fish now also possesses certain resistance and adaptive capacity, more adapts to artificial environment and artificial feed than the wild clown fish of fishing for, and possesses certain commercial value.”
But above not proper scale equalization of content.
Summary of the invention
For above-mentioned deficiency, technical problem to be solved by this invention is that research gropes to be suitable for each discontinuous running parameter that scale can reuse, and proposes the seedling-cultivating method of a kind of industrialization son clown fish.
The seedling-cultivating method of son clown fish provided by the invention, has the following steps:
A, parent fish rearing
Choose good, energetic, the undamaged son clown fish of a pair of body colour and put into breeding groove as parent population and cultivate separately, wherein female fish length is 6.5-7.8cm, and male fish length is 3.2-5cm, puts into the spawning ground of a crock as parent population in breeding groove;
In breeding groove, condition of water quality is: 26-28 DEG C of water temperatures, and salinity 28-32 ‰, dissolved oxygen is greater than 5mg/L, and ammonia-nitrogen content is lower than 0.1 mg/L;
Breeding groove is placed on indoor, intensity of illumination 2000Lux, light application time 14h/d;
Carry out fortification by the broken foot couple parent population of throw something and feed manufactured feed and oyster meat, method is the manufactured feed of respectively throwing something and feeding a time between 20 minutes between 20 minutes and before and after 14 o'clock before and after 8 o'clock every days, the broken end of oyster meat of respectively throwing something and feeding one time between 20 minutes between 20 minutes and before and after 15 o'clock before and after 11 o'clock every days, each feeding volume is respectively 3 ± 1% of fish body weight; Wherein manufactured feed is that every kilogram of basal feed adds astaxanthin 100mg and DHA150mg and Vc200mg and mixes, and described basal feed is commercially available fish feed.
B, incubating oosperm
Fertilized egg is the parent population natural spawning after fortification, and fertilized egg is viscid egg, is attached to crock inwall, and hatching is directly carried out in breeding groove;
In breeding groove, condition of water quality is constant;
Fertilized egg becomes while having silver color to flash after hatching, starts breeding groove to carry out shading at night on the same day, is hatched in the environment of complete darkness by prelarva;
C, larvae juvenile fish are cultivated
Larvae juvenile fish is cultivated and is continued to carry out in breeding groove, and in breeding groove, condition of water quality is constant;
C1, the bait of throwing something and feeding
After prelarva hatches, immediately parent population is removed and bait throwing in, bait is that density is 5-10 ind/mL, adds fresh marine chlorella simultaneously through the wheel animalcule of marine chlorella 24h strengthening, and density is 50000 ind/mL;
Prelarva hatches and plays the wheel animalcule through marine chlorella 24h strengthening of throwing something and feeding every day 2 times rear next day, feeding volume is that 1st-2d makes the wheel animalcule density in water body remain on 5-10 ind/mL, 3rd-5d makes the wheel animalcule density in water body remain on 2-3ind/mL, and 6d rises and stops throwing something and feeding wheel animalcule;
The 3d halogen worm that starts to throw something and feed, the halogen worm of throwing something and feeding is more than strengthening 12h with DHA and chlorella, feeding volume is that 3rd-6d keeps artemia density in water body at 1-2ind/mL, and the 7-8 d keeps artemia density in water body at 0.5-1ind/mL, and 9d rises and stops throwing something and feeding halogen worm;
The 7d copepoda that starts to throw something and feed, the 7-9 d keeps copepoda density in water body at 1ind/mL, and the 10-14 d keeps copepoda density in water body at 0.5ind/mL, the 15d copepoda that stops throwing something and feeding;
The meat gruel shape fresh food that 10d starts to throw something and feed the raw small animal making in the sea more than 25% by content of phospholipid, each feeding volume be fish heavy 3 ± 1%, number of times 10th-the 19d that throws something and feeds is 5-8 times/d, and the 20-25 is 2-3 times/d, and 26d rises and stops throwing something and feeding fresh food;
The 20d manufactured feed that starts to throw something and feed, the number of times of throwing something and feeding is 4-6 times/d, each feeding volume be fish heavy 2 ± 1%;
C2, change moisture cylinder
Prelarva hatches rear front 3d does not change water, inhales the end 1 time every day, and soil pick-up amount is 10%, only adds water, and adds water to former water level every day;
Since the 4th d, change water, quantity of exchanged water increases gradually, and to ensure that cultivating water quality remains on ammonia-nitrogen content lower than 0.1 mg/L, content of nitrite is lower than 0.01 mg/L;
15d divides cylinder, makes to cultivate density and is reduced to 1000-2000ind/m
3;
30d divides cylinder again, makes to cultivate density and is down to 400-500 ind/m
3, reduce mutually baiting between fry;
Cultivate through 60d, juvenile fish total length reaches 2-3cm, cultivates and finishes.
The seedling-cultivating method of son clown fish provided by the invention, proposed one can industrialized scale equalization route, compared with prior art, have more popularization, for the large-scale cultivation of son clown fish provides the foundation.
As another kind of scheme, the seedling-cultivating method of son clown fish provided by the invention, become after hatching in fertilized egg while having silver color to flash, before sunset on the same day, the crock that is attached with fertilized egg is transferred in hatching cylinder together with parent population, the shading of hatching cylinder is transferred back to crock breeding groove again together with parent population after prelarva hatches.Can avoid causing Gunther parent fish spawning irregular because parent fish rearing groove changes.
Described hatching cylinder is to be highly the glass jar of 50cm, and hatching cylinder is provided with water inlet pipe and overflow pipe, and spilling water pipe box has 80 mesh sieve tulles; Water inlet pipe regulating water quality, bolting silk net prevents that ovum runs off.The present invention's hatching cylinder used can effectively promote water body flow, reduces the internecine probability of growing seedlings, and is conducive to improve survival rate.
Described breeding groove height of water level is 28-35cm.
Described basal feed is the commercially available precious pellet of fish.
The raw small animal clam worm in the sea of described content of phospholipid more than 25% and razor clam.The parent population diet collocation that the present invention is selected, can effectively promote Gunther parent fish spawning, improves archiblast, and prelarva incubation rate reaches more than 90%.The collocation of live food of the present invention, can effectively improve survival rate and the growth rate of prelarva, and the body colour of juvenile fish is also more bright-coloured simultaneously.
Embodiment
Son clown fish seedling-cultivating method of the present invention, mainly contains 1, parent population pairing; 2, parent population accelerating; 3, incubating oosperm; 4, larvae juvenile fish is cultivated four steps.Concrete operation method is as follows:
A, parent population pairing
Choose good, energetic, the undamaged son clown fish of a pair of body colour as parent population, female fish length is 7cm, and male fish length is 4cm.They are put into the square glass parent population cylinder of 65cm × 65cm × 50cm and cultivate separately, in parent population cylinder, put into the spawning ground of a crock as parent population.Parent population cylinder is also breeding groove.
Raise two fishes more than the moon and occur that swimming altogether phenomenon represents successful matching.
B, parent population accelerating
The condition of water quality of parent fish rearing is: 28 DEG C of water temperatures, and salinity 28 ‰, dissolved oxygen is greater than 5mg/L, and ammonia-nitrogen content is lower than 0.1 mg/L.The fluorescent tube illumination of 20w for 20cm place, parent population cylinder top, illumination every day 14h.Throw something and feed every day each 2 times of the oyster of homemade manufactured feed and the shape that is cut into small pieces, Feeding time is respectively 8:00,14:00 and 11:00,17:00.
Through half a year, many raisings are laid eggs first again, and after this parent population lays eggs 1 time for every about 10 days.Lay eggs to be all placed on separately in hatching cylinder at every turn and cultivate, this example is got last laying eggs and is made description object.
C, hatching
Get the gained fertilized egg of laying eggs for the last time, totally 401 pieces.
Fertilized egg is the parent population natural spawning after fortification, and fertilized egg is viscid egg, is attached to crock inwall, and hatching is directly carried out in parent population cylinder.The condition of water quality of incubating oosperm is: 26 DEG C of water temperatures, and salinity 30 ‰, dissolved oxygen is greater than 5mg/L, and ammonia-nitrogen content is lower than 0.1 mg/L.Hatching after 8d, it is silver-shiny that fertilized egg becomes, and in 16:00, the crock that is attached with fertilized egg transferred to together with parent population in the square hatching cylinder of 65cm × 65cm × 50cm and hatched, and this square hatching cylinder is opaque and have an opaque shading cover.Hatch in 20:00 prelarva, after prelarva hatches, crock is transferred back to together with parent population to parent population cylinder.
Incubate to obtain prelarva 370 tails.Incubate yield 92.3%.
D, larvae juvenile fish are cultivated
Parent population cylinder water level 30cm, condition of water quality is: 27 ± 1 DEG C of water temperatures, salinity 30 ± 2 ‰, dissolved oxygen is greater than 5mg/L, and ammonia-nitrogen content is lower than 0.1 mg/L.
After prelarva hatches, start bait throwing in, prelarva membrane bait on the same day is that density is 5-10 ind/mL, adds fresh marine chlorella simultaneously through the wheel animalcule of marine chlorella strengthening 24h, and density is 50000 ind/mL.
Prelarva hatches best ingest time 6:00-8:00 in the morning of rising rear next day prelarva and two time sections of 3:00-5:00 in afternoon bait of throwing something and feeding.Throw something and feed number of times from respectively once, progressively increase number of times by feeding volume requirement.Before throwing something and feeding in 3d, first inhale the end 1 time, soil pick-up amount is water level 10%, adds water to former water level after soil pick-up, and the discharge outlet of soil pick-up is collected with 60 order nets, to check.The 4th d rises and starts to increase soil pick-up amount, makes water body obtain changing water, and quantity of exchanged water increases gradually, is increased to 200% from 20%, and to ensure that cultivating water quality remains on ammonia-nitrogen content lower than 0.1 mg/L, content of nitrite is lower than 0.01 mg/L.
1st, 2d, throws something and feeds through the wheel animalcule of marine chlorella strengthening 24h, and feeding volume is that in maintenance water, wheel animalcule density is 5-10ind/mL, and after throwing, 1h checks the situation of ingesting.
3rd, 4,5d, throw something and feed through the wheel animalcule of marine chlorella strengthening 24h with through with DHA and halogen worm more than chlorella strengthening 12h, feeding volume is that to keep wheel animalcule density in water be 2-3ind/mL, artemia density 1-2ind/mL.
The 6th day, stop the wheel animalcule of throwing something and feeding, through continuing to remain on 0.5-1ind/mL with the feeding volume of DHA and halogen worm more than chlorella strengthening 12h.
7th, 8 d, throw something and feed through halogen worm and copepoda with more than DHA and chlorella strengthening 12h, feeding volume be keep artemia density in water body in 0.5-1ind/mL, copepoda density at 1ind/mL.
9d, the halogen worm that stops throwing something and feeding, the copepoda density in the feeding volume continuation maintenance water body of copepoda is at 1ind/mL.
10th-14d, the meat gruel of throwing something and feeding copepoda and being broken into by fresh foods such as clam worm, razor clam meat, the feeding volume of copepoda keeps density in water body at 0.5ind/mL, 5-8 times/d of the feeding volume of fresh food, each feeding volume is 3 ± 1% of fish gross weight.
15d, point cylinder, chooses fry large individuality, makes to cultivate density and is reduced to 200 tails; Stop the copepoda of throwing something and feeding, the feeding volume of the meat gruel being broken into by fresh foods such as clam worm, razor clam meat keeps 5-8 times/d, and each feeding volume is 3 ± 1% of fish gross weight.
16th-19d, continuation amount keeps 5-8 times/d meat gruel being broken into by fresh foods such as clam worm, razor clam meat of throwing something and feeding, and each feeding volume is 3 ± 1% of fish gross weight.
20th-25d, meat gruel and the manufactured feed of throwing something and feeding and being broken into by fresh foods such as clam worm, razor clam meat, the feeding volume of meat gruel keeps 2-3 times/d, and each feeding volume is 3 ± 1% of fish gross weight.The feeding volume of manufactured feed is that number of times is 4-6 times/d, and each feeding volume is 2 ± 1% of fish gross weight.
26d rises, and stops the meat gruel of throwing something and feeding, and the feeding volume of manufactured feed continues to keep 4-6 times/d, and each feeding volume is 2 ± 1% of fish gross weight.
30d divides cylinder again, and fry large individuality is chosen, and makes to cultivate density and is down to 100 tails.
Within 60 days, cultivate altogether afterwards 225 of son clown fries, average length is 2.67cm, survival rate 60.8%.
Claims (6)
1. a seedling-cultivating method for son clown fish, is characterized in that having the following steps:
A, parent fish rearing
Choose good, energetic, the undamaged son clown fish of a pair of body colour and put into breeding groove as parent population and cultivate separately, wherein female fish length is 6.5-7.8cm, and male fish length is 3.2-5cm, puts into the spawning ground of a crock as parent population in breeding groove;
In breeding groove, condition of water quality is: 26-28 DEG C of water temperatures, and salinity 28-32 ‰, dissolved oxygen is greater than 5mg/L, and ammonia-nitrogen content is lower than 0.1 mg/L;
Breeding groove is placed on indoor, intensity of illumination 2000Lux, light application time 14h/d;
Carry out fortification by the broken foot couple parent population of throw something and feed manufactured feed and oyster meat, method is the manufactured feed of respectively throwing something and feeding a time between 20 minutes between 20 minutes and before and after 14 o'clock before and after 8 o'clock every days, the broken end of oyster meat of respectively throwing something and feeding one time between 20 minutes between 20 minutes and before and after 15 o'clock before and after 11 o'clock every days, each feeding volume is respectively 3 ± 1% of fish body weight; Wherein manufactured feed is that every kilogram of basal feed adds astaxanthin 100mg and DHA150mg and Vc200mg and mixes, and described basal feed is commercially available fish feed;
B, incubating oosperm
Fertilized egg is the parent population natural spawning after fortification, and fertilized egg is viscid egg, is attached to crock inwall, and hatching is directly carried out in breeding groove;
In breeding groove, condition of water quality is constant;
Fertilized egg becomes while having silver color to flash after hatching, starts breeding groove to carry out shading at night on the same day, is hatched in the environment of complete darkness by prelarva;
C, larvae juvenile fish are cultivated
Larvae juvenile fish is cultivated and is continued to carry out in breeding groove, and in breeding groove, condition of water quality is constant;
C1, the bait of throwing something and feeding
After prelarva hatches, immediately parent population is removed and bait throwing in, bait is that density is 5-10 ind/mL, adds fresh marine chlorella simultaneously through the wheel animalcule of marine chlorella 24h strengthening, and density is 50000 ind/mL;
Prelarva hatches and plays the wheel animalcule through marine chlorella 24h strengthening of throwing something and feeding every day 2 times rear next day, feeding volume is that 1st-2d makes the wheel animalcule density in water body remain on 5-10 ind/mL, 3rd-5d makes the wheel animalcule density in water body remain on 2-3ind/mL, and 6d rises and stops throwing something and feeding wheel animalcule;
The 3d halogen worm that starts to throw something and feed, the halogen worm of throwing something and feeding is more than strengthening 12h with DHA and chlorella, feeding volume is that 3rd-6d keeps artemia density in water body at 1-2ind/mL, and the 7-8 d keeps artemia density in water body at 0.5-1ind/mL, and 9d rises and stops throwing something and feeding halogen worm;
The 7d copepoda that starts to throw something and feed, the 7-9 d keeps copepoda density in water body at 1ind/mL, and the 10-14 d keeps copepoda density in water body at 0.5ind/mL, the 15d copepoda that stops throwing something and feeding;
The meat gruel shape fresh food that 10d starts to throw something and feed the raw small animal making in the sea more than 25% by content of phospholipid, each feeding volume be fish heavy 3 ± 1%, number of times 10th-the 19d that throws something and feeds is 5-8 times/d, and the 20-25 is 2-3 times/d, and 26d rises and stops throwing something and feeding fresh food;
The 20d manufactured feed that starts to throw something and feed, the number of times of throwing something and feeding is 4-6 times/d, each feeding volume be fish heavy 2 ± 1%;
C2, change moisture cylinder
Prelarva hatches rear front 3d does not change water, inhales the end 1 time every day, and soil pick-up amount is 10%, only adds water, and adds water to former water level every day;
Since the 4th d, change water, quantity of exchanged water increases gradually, and to ensure that cultivating water quality remains on ammonia-nitrogen content lower than 0.1 mg/L, content of nitrite is lower than 0.01 mg/L;
15d divides cylinder, makes to cultivate density and is reduced to 1000-2000ind/m
3;
30d divides cylinder again, makes to cultivate density and is down to 400-500 ind/m
3, reduce mutually baiting between fry;
Cultivate through 60d, juvenile fish total length reaches 2-3cm, cultivates and finishes.
2. the seedling-cultivating method of son clown fish as claimed in claim 1, it is characterized in that becoming after hatching in fertilized egg while having silver color to flash, before sunset on the same day, the crock that is attached with fertilized egg is transferred in hatching cylinder together with parent population, the shading of hatching cylinder is transferred back to crock breeding groove again together with parent population after prelarva hatches.
3. the seedling-cultivating method of son clown fish as claimed in claim 2, it is characterized in that hatching cylinder is to be highly the glass jar of 50cm, and hatching cylinder is provided with water inlet pipe and overflow pipe, and spilling water pipe box has 80 mesh sieve tulles; Water inlet pipe regulating water quality, bolting silk net prevents that ovum runs off.
4. the seedling-cultivating method of the son clown fish as described in claim 1 or 2 or 3, is characterized in that breeding groove height of water level is 28-35cm.
5. the seedling-cultivating method of son clown fish as claimed in claim 1, is characterized in that described basal feed is the commercially available precious pellet of fish.
6. the seedling-cultivating method of son clown fish as claimed in claim 1, is characterized in that the raw small animal clam worm in the sea of described content of phospholipid more than 25% and razor clam.
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| CN106472374A (en) * | 2016-09-14 | 2017-03-08 | 陈晓蕾 | Clown's fish fertilized egg artificial incubation specialized simulation pectoral fin agitates equipment and hatching method |
| CN107873574A (en) * | 2017-09-18 | 2018-04-06 | 芜湖新华联文化旅游开发有限公司 | A kind of clown fish is in subtropical zone cultural method |
| CN109275600A (en) * | 2018-11-08 | 2019-01-29 | 中国水产科学研究院黄海水产研究所 | A kind of Hapalogenys nitens indoor raising of seedling method |
| CN113455428A (en) * | 2021-08-17 | 2021-10-01 | 中国水产科学研究院东海水产研究所 | Artificial breeding method for Mafu fish |
| CN115104559A (en) * | 2022-07-22 | 2022-09-27 | 福建省水产研究所（福建水产病害防治中心） | Method for mutagenizing black male clown fish body color to become red by ARTP (ARTP) |
| TWI815169B (en) * | 2021-08-23 | 2023-09-11 | 國立屏東科技大學 | Method for cultivation of anemonefish to improve efficiency of spawning |
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| CN113455428B (en) * | 2021-08-17 | 2022-05-27 | 中国水产科学研究院东海水产研究所 | Artificial breeding method for Mafu fish |
| TWI815169B (en) * | 2021-08-23 | 2023-09-11 | 國立屏東科技大學 | Method for cultivation of anemonefish to improve efficiency of spawning |
| CN115104559A (en) * | 2022-07-22 | 2022-09-27 | 福建省水产研究所（福建水产病害防治中心） | Method for mutagenizing black male clown fish body color to become red by ARTP (ARTP) |
| CN115104559B (en) * | 2022-07-22 | 2023-10-27 | 福建省水产研究所（福建水产病害防治中心） | Method for changing body color of black princess clown fish into red through ARTP mutagenesis |
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