CN103125413A - Artificial maturing method for exopalaemon carinicauda in anestrous seasons - Google Patents
Artificial maturing method for exopalaemon carinicauda in anestrous seasons Download PDFInfo
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- CN103125413A CN103125413A CN2013100640056A CN201310064005A CN103125413A CN 103125413 A CN103125413 A CN 103125413A CN 2013100640056 A CN2013100640056 A CN 2013100640056A CN 201310064005 A CN201310064005 A CN 201310064005A CN 103125413 A CN103125413 A CN 103125413A
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- 238000000034 method Methods 0.000 title claims abstract description 37
- 241000851180 Palaemon carinicauda Species 0.000 title claims description 35
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 44
- 230000005070 ripening Effects 0.000 claims abstract description 11
- 230000008569 process Effects 0.000 claims abstract description 9
- 230000033228 biological regulation Effects 0.000 claims abstract description 6
- 241000238557 Decapoda Species 0.000 claims description 37
- 238000005286 illumination Methods 0.000 claims description 26
- 230000003203 everyday effect Effects 0.000 claims description 16
- 235000013601 eggs Nutrition 0.000 claims description 7
- 230000000384 rearing effect Effects 0.000 claims description 7
- 235000013305 food Nutrition 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 230000001568 sexual effect Effects 0.000 claims description 5
- 230000008859 change Effects 0.000 claims description 4
- 235000013372 meat Nutrition 0.000 claims description 4
- 241000238366 Cephalopoda Species 0.000 claims description 3
- 230000010539 reproductive behavior Effects 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 230000037396 body weight Effects 0.000 claims 3
- 230000015556 catabolic process Effects 0.000 claims 1
- 238000011109 contamination Methods 0.000 claims 1
- 238000006731 degradation reaction Methods 0.000 claims 1
- 239000002245 particle Substances 0.000 claims 1
- 238000009395 breeding Methods 0.000 abstract description 12
- 230000001488 breeding effect Effects 0.000 abstract description 12
- 230000026109 gonad development Effects 0.000 abstract description 7
- 235000016709 nutrition Nutrition 0.000 abstract description 4
- 241001149925 Fenneropenaeus indicus Species 0.000 abstract 4
- 241000530454 Litopenaeus schmitti Species 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 230000000630 rising effect Effects 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 229920003023 plastic Polymers 0.000 description 8
- 239000004033 plastic Substances 0.000 description 8
- 230000012447 hatching Effects 0.000 description 7
- 239000004568 cement Substances 0.000 description 6
- 230000002159 abnormal effect Effects 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 230000034004 oogenesis Effects 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000004907 gland Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241001247197 Cephalocarida Species 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000010612 desalination reaction Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000029052 metamorphosis Effects 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000029264 phototaxis Effects 0.000 description 1
- 239000012286 potassium permanganate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 1
- 235000019345 sodium thiosulphate Nutrition 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
本发明涉及一种脊尾白虾非繁殖季节人工促熟方法，具体地说是一种利用温度、光照周期调控并辅以营养强化以实现脊尾白虾秋冬季节人工繁殖的方法；主要包括水温控制、光照周期调控和营养强化。水温控制是利用温控设备模拟春季水温逐渐上升的过程；光照周期调控是利用日光灯和定时器模拟春季光照周期逐渐延长的过程；营养强化是投喂沙蚕等鲜活饵料强化性腺发育过程中的营养供给。在此条件下，经过2-3的周培育，脊尾白虾可顺利产卵繁殖。本发明通过模拟自然条件下春季水温逐渐上升、光照周期逐渐延长的特点实现了脊尾白虾非繁殖季节的性腺发育及产卵繁殖，从而达到控制脊尾白虾周年繁殖的目的。The invention relates to a method for artificially promoting ripening of white prawns in non-breeding seasons, specifically a method for artificially breeding white prawns in autumn and winter by using temperature and photoperiod regulation and supplemented with nutritional enhancement; mainly including water temperature control, Photoperiod regulation and nutritional fortification. Water temperature control is the process of using temperature control equipment to simulate the gradual rise of water temperature in spring; photoperiod regulation is the process of using fluorescent lamps and timers to simulate the process of gradually extending the spring photoperiod; nutritional enhancement is the process of feeding clamworm and other fresh bait to strengthen the gonad development process. Nutrient supply. Under these conditions, after 2-3 weeks of cultivation, the white shrimp can spawn and reproduce smoothly. The invention realizes the gonad development and spawning reproduction of the white prawn in the non-breeding season by simulating the characteristics of the spring water temperature gradually rising and the light cycle gradually extending under natural conditions, so as to achieve the purpose of controlling the annual reproduction of the white prawn.
Description
Technical field
The present invention relates to a kind of Exopalaemon carinicauda anestrous season artificial ripening method, specifically a kind ofly utilize temperature, periodicity of illumination to regulate and control and be aided with fortification to realize the method for Exopalaemon carinicauda autumn and winter season egg reproduction, belong to technical field of aquaculture.Utilize this technology can realize anniversary breeding and the production unit anti-season seed rearing of Exopalaemon carinicauda under laboratory condition.
Background technology
Exopalaemon carinicauda is the shrimps of dwelling at the bottom of warm band sea area, with yellow, Bohai Sea output is the highest, is one of distinctive 3 kinds of economic shrimps of China, its output is only second to Chinese prawn and Chinese shrimp.The Exopalaemon carinicauda environmental suitability is strong, growth is fast, meat is good, economic worth is high, and fanning economics is considerable, is potential aquaculture shrimps kind, and its cultured output has reached 1/3rd left and right of Deposits in Eastern Coastal China polycultured ponds gross yield at present.Exopalaemon carinicauda is low temperature resistant, and suitable salt scope is wide, through desalination, can in freshwater, cultivate, and it cultivates wide scope, potentiality are large.In the area that geothermal energy resources are arranged, can carry out especially producing in anti-season of autumn and winter cultivation large specification listing product, the economic benefit multiplication.In addition, Exopalaemon carinicauda has short, characteristics such as individuality is little, health is translucent of generation cycle, is one of very potential crustacean research mode animal exploitation kind.But, in autumn and winter season, along with the decline gradually of water temperature, the reproductive behavior of Exopalaemon carinicauda finishes gradually, in now Exopalaemon carinicauda cultivation seed production and laboratory, obtaining of Exopalaemon carinicauda commitment experiment material will be very difficult.For this reason, research Exopalaemon carinicauda gonad development rule, realize that Exopalaemon carinicauda is very crucial in sexual gland continuation growth the anniversary breeding under normal spawning and laboratory condition of autumn and winter season.
The object of the invention is to by temperature control, periodicity of illumination regulation and control and three kinds of effective means of fortification, realize anniversary breeding and the production unit anti-season seed rearing of Exopalaemon carinicauda under laboratory condition.
Summary of the invention
The present invention is directed to the decline gradually along with water temperature of natural conditions lower autumn and winter seasons, the reproductive behavior of Exopalaemon carinicauda finishes, in Exopalaemon carinicauda cultivation seed production and laboratory, Exopalaemon carinicauda commitment experiment material will obtain very difficult problem, developed and a kind ofly risen gradually by simulating natural condition water temperature in lower spring, the characteristics that periodicity of illumination extends have gradually realized gonad development and the egg reproduction of Exopalaemon carinicauda anestrous season, thereby reach the method for the anniversary breeding of Exopalaemon carinicauda, for Exopalaemon carinicauda laboratory anniversary breeding and production unit anti-season seed rearing provide technical support.
For achieving the above object, technical scheme of the present invention is as follows:
Choose the close shrimp that reaches the sexual maturity specification, the characteristics that simulating natural condition water temperature in lower spring rises gradually, periodicity of illumination extends gradually also are aided with fortification and have realized that Exopalaemon carinicauda can grow and lay eggs at the anestrous season sexual gland, and concrete grammar is:
(1) water temperature is controlled: utilize the temperature control device to improve gradually the cultivation water temperature.
(2) periodicity of illumination is regulated: the combination that utilizes indoor daylight lamp and timer is the prolonging light cycle day by day.
(3) fortification: by fresh foods such as the clam worm of throwing something and feeding, squid and bright clam meats, guarantee the demand of gonad development to holozoic nutrition.
With this understanding, through the Zhou Peiyu of 2-3, Exopalaemon carinicauda is egg reproduction smoothly.The present invention has realized gonad development and the egg reproduction of Exopalaemon carinicauda anestrous season by the characteristics that simulating natural condition water temperature in lower spring rises gradually, periodicity of illumination extends gradually, thereby reaches the purpose of controlling the breeding of Exopalaemon carinicauda anniversary.The present invention realizes the breeding of laboratory anniversary and production unit anti-season seed rearing for Exopalaemon carinicauda technical support is provided.
Water temperature of the present invention improves process should be slowly orderly, before water temperature≤20 ℃ every day 2 ℃ (raising respectively in the morning, the afternoon 1 ℃) left and right of heating up, and after in water temperature>20 ℃ an every day is advisable in the 1 ℃ of left and right of heating up, water temperature maintains this water temperature and no longer improves after reaching 25 ℃.
Periodicity of illumination control method of the present invention is: (the extend 20 minutes every day) periodicity of illumination that utilizes the combination of indoor daylight lamp and timer to extend day by day, water surface intensity of illumination is 500-1000lx, reaches 12h(illumination every day to light application time: maintain this light application time in the time of dark=12h:12h) and no longer extend.
Fortification method of the present invention is: increase the supply of the fresh foods such as clam worm, squid and bright clam meat in the Articial bait making of throwing something and feeding, the ratio of Articial bait making and fresh food is advisable with 1:3.
Articial bait making used is common commercially available prawn manufactured feed, by Guangdong Heng Xing feed corporation,Ltd, is produced.
Major advantage of the present invention has:
1, the present invention has abandoned traditional wipe out one-sided optic stalk accelerating method and adopting water temperature under simulating natural condition to raise and periodicity of illumination prolongation method promotes the Exopalaemon carinicauda gonad development, having advantages of simple and efficiently, is to be applicable to very much the less economic shrimps accelerating methods of build such as Exopalaemon carinicauda.
2, the raising of the water temperature in the present invention is slowly controlled, can determine temperature raising every day amplitude according to actual production (or experiment) preparation, has advantages of that controllability is good, planned strong.
3, by the nursery pond (5m of little water body
3) accelerating, grow seedlings and attempt and laboratory plastics tank (150-200L) accelerating, hatching experiment result show, the Exopalaemon carinicauda hatchability of fertile eggs obtained by this accelerating method and the data no significant difference of larval metamorphosis survival rate and mating season, can be for actual production.
Embodiment
Describe technical method of the present invention in detail below by specific embodiment:
Embodiment: the indoor cement pit of Exopalaemon carinicauda and plastics tank accelerating, breeding experiment
The present embodiment carries out according to following steps: prepare (1): the Brackish water Pond that strong chlorine oil is disinfected (15 ± 1 ‰) is through the sodium thiosulfate neutralization and fully after aeration, pump into 3 indoor cement pits (3.0 * 1.8 * 1.2m, effectively the water body 5m disinfected through potassium permanganate
3) in standby, (2) close shrimp enters pond (or entering groove): on November 20th, 2012, choose body surface smooth pond culture Exopalaemon carinicauda complete, that grow up in about 4cm, average weight 1.5g without sick and wounded, body and put into above-mentioned wherein 2 indoor cement pits (3Kg/ pond, density 200 tails/m
2left and right) in, separately getting 60 tail parent shrimp (female-male proportion 2:1) mean allocation to 2 a plastics tank (70 * 55 * 45cm) stablizes 7 days, keep during this time natural temperature, Articial bait making (" permanent emerging " the board prawn manufactured feed of throwing something and feeding every day, protein content 40%, 5% experiment shrimp gross weight), drag for away dead shrimp in time and suitably change water according to the water quality situation, (3) water temperature is controlled: close shrimp enters pond stablize 7 days after, utilizing Korea S to produce OKE-6710HF type temperature controller (cement pit and plastics tank are respectively with 2KW and the heating of 300W heating rod) slowly heats up, 1 ℃ (2 ℃/day) respectively heat up morning and afternoon every day, after intensification in 4 days arrives 20 ℃, every day, increasing extent of temperature changed 1 ℃ into, maintaining this water temperature again after intensification in 5 days arrives 25 ℃ no longer improves, during this time, another does not put the synchronous use that heats up and change water as the close shrimp of experiment of cement pit of shrimp, (4) regulation and control of periodicity of illumination: start the day (illumination: dark=10h:14h) of raising from cultivating water temperature, utilize the combination of indoor daylight lamp and timer, every day, prolonging light was 20 minutes, and after 6 days, periodicity of illumination is adjusted into 12h:12h, after this maintains this periodicity of illumination, (5) fortification: in intensification, prolonging light, throw something and feed every day clam worm and Articial bait making (mass ratio 3:1) each 1 time, and adjust in right amount (increase or reduce) feeding volume according to the situation of ingesting, (6) collection of oogenesis parent shrimp rearing and the young: whole experimental session, inhale bottom blowdown 1 time by the way of siphon every day, and look the water quality situation and suitably change water, through 15-20 days, cultivate, the parent shrimp is oogenesis successively, and the collection method of oogenesis parent shrimp rearing and the young is carried out according to another patent of invention of applicant " a kind of method of obvious raising Exopalaemon carinicauda artificial breeding survival rate " (application number 201210015976.7) described method, (7) young is cultivated: less because testing close shrimp, parent shrimp oogenesis and hatching sync rates are lower, on December 16th, 2012 and 17 days, the young in 2 cement pits is collected, mixes respectively, get the 20000 tail young that the good energetic young of phototaxis counts to get and transfer in 2 150L plastics tanks (effective water body 100L grows seedlings) and cultivate, young density 200,000/m
3, adopt Artemia nauplii and Articial bait making conventional seedbed system, under 25 ℃, salinity 15 ‰ conditions, the young is abnormal through about 11 days is the young shrimp (P1) of the long 5.5-6mm of body, its abnormal survival rate is respectively 66.5% and 82%, embryo's eyespot in the plastics tank is obvious, the shrimp with ovums list tail of approaching hatching is transferred to and is positioned at less plastics tank (50 * 40 * 35cm, effective water body 50L) in the hatching net (15 * 15 * 20cm) of a jiao, get altogether 4 tails for experiment, the young collection method suitably changed by foregoing invention patent (application number 201210015976.7), be about to top, position and the black plastic cloth shading for side at little tank (50L) hatching net place, produce intensity of illumination with the 20W electricity-saving lamp poor, illumination lures the young extremely away from hatching net one end, remove close shrimp after hatching fully Deng the young, the counting young and routinely seedling-cultivating method cultivate the young, at 25 ℃, under salinity 15 ‰ conditions, the young is the abnormal young shrimp (P1) for the long 5.5-6mm of body through about 11 days, its average abnormal survival rate is 85.2%.
Claims (7)
1. an Exopalaemon carinicauda anestrous season artificial ripening method is characterized in that:
(1) selection of close shrimp and foster temporarily: anestrous season, select to have reached the close shrimp of sexual maturity specification (female: as hero=2-3:1) to put into the parent shrimp rearing pond and support temporarily 7-10 days, keep during this time natural temperature, periodicity of illumination (depending on natural conditions at that time), the Articial bait making remove contamination in time, change water of throwing something and feeding every day.
(2) artificial ripening: by simulating natural condition, breed the characteristics that the outset season water temperature rises gradually, periodicity of illumination extends gradually lower spring, utilize temperature, periodicity of illumination regulation and control to be aided with fortification simultaneously and to realize the egg reproduction of Exopalaemon carinicauda at anestrous season, concrete regulate and control method comprises that water temperature is controlled, periodicity of illumination is regulated and fortification.
2. according to artificial ripening method claimed in claim 1, it is characterized in that:
In the period that the Exopalaemon carinicauda reproductive behavior that anestrous season refers under natural conditions, autumn and winter season, degradation caused because water temperature descends, under the bait richness stops, the water temperature in this period should be lower than 20 ℃, and day illumination time is shorter than 12h.
3. according to artificial ripening method claimed in claim 1, it is characterized in that:
The individual specification of alternative close shrimp is minimum should reach sexual maturity standard (body length >=4cm, body weight >=1g), and female shrimp is with the long 5-6cm of body, and body weight 2-3g is advisable, and male shrimp is with the long 4-5cm of body, and body weight 1-2g is advisable; The parent shrimp answers that body colour is normal, body surface smooth without attachment, outward appearance without sick and wounded.
4. according to artificial ripening method claimed in claim 1, it is characterized in that:
In the water temperature control procedure, the raising process of parent shrimp cultivation water temperature should be slowly orderly, before water temperature≤20 ℃ every day 2 ℃ (raising respectively in the morning, the afternoon 1 ℃) left and right of heating up, and after in water temperature>20 ℃ an every day is advisable in the 1 ℃ of left and right of heating up, water temperature maintains this water temperature and no longer improves after reaching 25 ℃.
5. according to artificial ripening method claimed in claim 1, it is characterized in that:
In the periodicity of illumination adjustment process, (the extend 20 minutes every day) periodicity of illumination that utilizes the combination of indoor daylight lamp and timer to extend day by day, water surface intensity of illumination is 500-1000lx, reaches 12h(illumination every day to light application time: maintain this light application time in the time of dark=12h:12h) and no longer extend.
6. according to artificial ripening method claimed in claim 1, it is characterized in that:
In the selection of close shrimp and support temporarily and the fortification process in, Articial bait making used is common commercially available prawn manufactured feed, protein content 40% left and right, particle size is advisable to be applicable to the long 4-5cm shrimp of body;
Described fresh food is one or two or more kinds in clam worm, squid and bright clam meat.
7. according to the described artificial ripening method of claim 1 or 6, it is characterized in that:
The mass ratio of Articial bait making and fresh food is advisable with 1:3, and the total amount of throwing something and feeding (Articial bait making adds fresh food) is the 8-10% of close shrimp gross weight.
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| CN2013100640056A CN103125413A (en) | 2013-02-28 | 2013-02-28 | Artificial maturing method for exopalaemon carinicauda in anestrous seasons |
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| CN2013100640056A CN103125413A (en) | 2013-02-28 | 2013-02-28 | Artificial maturing method for exopalaemon carinicauda in anestrous seasons |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103444608A (en) * | 2013-09-12 | 2013-12-18 | 中国水产科学研究院黄海水产研究所 | Reproductive regulation method of wolf eel parents |
| CN103766261A (en) * | 2014-02-26 | 2014-05-07 | 中国水产科学研究院黄海水产研究所 | Annular continuous multi-batch artificial culture method for palaemon carinicauda |
| CN107372252A (en) * | 2017-08-29 | 2017-11-24 | 大连海洋大学 | Extra large huge legendary turtle shrimp offspring seed cultivation method |
| CN115443930A (en) * | 2022-08-31 | 2022-12-09 | 渤海水产育种(海南)有限公司 | Parent shrimp breeding method for litopenaeus vannamei with stable spawning period |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1806535A (en) * | 2006-01-13 | 2006-07-26 | 厦门大学 | Method for promoting common shrimp ovary maturity and spawning |
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2013
- 2013-02-28 CN CN2013100640056A patent/CN103125413A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1806535A (en) * | 2006-01-13 | 2006-07-26 | 厦门大学 | Method for promoting common shrimp ovary maturity and spawning |
Non-Patent Citations (2)
| Title |
|---|
| 张庆文等: "中国对虾亲虾反季节培育技术的初步研究", 《海洋水产研究》, vol. 24, no. 3, 30 September 2003 (2003-09-30), pages 34 - 37 * |
| 王春琳等: "脊尾白虾Palaemon carinicauda人工促熟的初步研究", 《浙江海洋学院学报》, vol. 14, no. 4, 31 December 1995 (1995-12-31), pages 243 - 246 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103444608A (en) * | 2013-09-12 | 2013-12-18 | 中国水产科学研究院黄海水产研究所 | Reproductive regulation method of wolf eel parents |
| CN103444608B (en) * | 2013-09-12 | 2015-02-04 | 中国水产科学研究院黄海水产研究所 | Reproductive regulation method of wolf eel parents |
| CN103766261A (en) * | 2014-02-26 | 2014-05-07 | 中国水产科学研究院黄海水产研究所 | Annular continuous multi-batch artificial culture method for palaemon carinicauda |
| CN103766261B (en) * | 2014-02-26 | 2015-08-26 | 中国水产科学研究院黄海水产研究所 | Exopalaemon carinicauda 1 year continuous many batches of artificial cultivating method |
| CN107372252A (en) * | 2017-08-29 | 2017-11-24 | 大连海洋大学 | Extra large huge legendary turtle shrimp offspring seed cultivation method |
| CN107372252B (en) * | 2017-08-29 | 2020-08-04 | 大连海洋大学 | Method for cultivating young crayfish |
| CN115443930A (en) * | 2022-08-31 | 2022-12-09 | 渤海水产育种(海南)有限公司 | Parent shrimp breeding method for litopenaeus vannamei with stable spawning period |
| CN115443930B (en) * | 2022-08-31 | 2023-09-05 | 渤海水产育种(海南)有限公司 | Parent shrimp cultivation method for litopenaeus vannamei with stable spawning period |
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Application publication date: 20130605 |