CN107581106B - A kind of artificial fecundation method of platax orbicularis - Google Patents

A kind of artificial fecundation method of platax orbicularis Download PDF

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CN107581106B
CN107581106B CN201710891104.XA CN201710891104A CN107581106B CN 107581106 B CN107581106 B CN 107581106B CN 201710891104 A CN201710891104 A CN 201710891104A CN 107581106 B CN107581106 B CN 107581106B
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water
lrh
platax
orbicularis
fertilized eggs
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CN107581106A (en
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蔡春有
黄海
蔡有森
蔡四川
蔡建顺
蔡惠明
蔡金泉
陈燕
马军
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Hainan Chenhai Aquatic Co Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention discloses a kind of artificial fecundation methods of platax orbicularis, include the following steps:1 Juvenile stage, the net cage reinforced cultivating of 2 parent populations, 3 parent populations hasten parturition, the collection of 4 fertilized eggs and processing, 5 incubating oosperms, 6 seeds pond rearing.The present invention promotes parent population to lay eggs by the parent culture to platax orbicularis, then goes out prelarva with incubating oosperm, cultivates at can create conditions for the fingerling of cultivation for the large-scale farming of platax orbicularis.Meanwhile the present invention is that seawater fish artificial increasing cultivation increases new varieties, the recovery and aquaculture industry development to platax orbicularis natural resources play the role of extremely important.

Description

A kind of artificial fecundation method of platax orbicularis
Technical field
The present invention relates to technical field of aquaculture, and in particular to a kind of artificial fecundation method of platax orbicularis.
Background technique
Platax orbicularis (scientific name:Platax orbicularis) also known as circle eye Spanish mackerel, circle bat etc., it is Perciformes, pierces tail fish Suborder, orbfish section, a kind of seawater fish in the life of torrid areas immediate offshore area that Spanish mackerel belongs to are distributed in India-Western Pacific Warm water waters, North gets South Japan, central Pacific island are arrived up to Australia, east in south, west to the eastern bank in Red sea and Africa, China Originate in the South Sea.
Platax orbicularis lives in 5-30 meters deeply, has the sea area that slightly mud is protected, haunts about near the accident shipwreck of deep-sea. Juvenile fish mainly inhabites in shallow sea or river mouth waters, because body colour is withered and yellow brown, meeting mimicry is hidden at dead leaf shape in floating on water Under object, often alone or groupuscule travelling in lagoon inner bay.Adult fish then gos deep into about 30 meters of Coral Reef Regions below, on its peripheral slope It is in pairs or movable in groups.Circle eye Spanish mackerel belongs to the omnivorous fish of day row, is mainly with algae, invertebrate or small fish Food.Platax orbicularis juvenile fish fine figure, can be used as ornamental fish;Adult fish is then edible, delicious meat.
Due to excessive fishing, so that the population quantity of platax orbicularis is greatly decreased, the market supply is limited, is unable to satisfy city The demand of field.Currently, the artificial scale breeding technology of platax orbicularis has not yet to see and has been reported that.Artificial scale breeding technology not at Ripe is to restrict its one of key factor for propagating industry development artificially.
Summary of the invention
It is an object of that present invention to provide a kind of artificial fecundation methods of platax orbicularis to be promoted by the parent culture to platax orbicularis So that parent population is laid eggs, then goes out prelarva with incubating oosperm, cultivates at can establish for the fingerling of cultivation for large-scale cultivation platax orbicularis Basis.
A kind of artificial fecundation method of platax orbicularis, includes the following steps:
(1) Juvenile stage
Parental source is the platax orbicularis of the capture of natural sea area or raising in cage, selects 4 more than age, weight 3.0-5.0kg, body Type is normal, disease-free, energetic, and the good platax orbicularis of gonad development is as parent;
(2) aquaculture net cage parent population reinforced cultivating
Select wind sheltering condition is good, water flow is unimpeded, depth of water 5m or more, sea area flow velocity less than 0.1m/s inner bay or shallow sea set Aquaculture net cage is set, 3 months progress parent population reinforced cultivatings before mating period;Artificial expanding compound feed is fed daily twice, upper, It in each 1 time of afternoon, is satiated with food and feeds, feed can add 0.2% fish oil, 0.5% multi-vitamins and 0.2% vitamin E, promote sexual gland Development;
(3) parent population is hastened parturition
It hastens parturition at sea to lay eggs and carry out in net cage, start April to progressively reach sexual gland maturation by the parent population of reinforced cultivating, It can carry out artificial induced spawning;Oxytocic hormone uses human chorionic gonadtropin HCG, ovulation induction No. 2 LRH-A2 of element and ovulation induction element 3 Number LRH-A3, injection oxytocic hormone are injected using dorsal muscles, are put into and are laid eggs in net cage after injection, through hormone induction 30-38h, allow it Naturally smart ovum, fertilization are discharged;
(4) collection and processing of fertilized eggs
After parent population oviposition, prepare 2 0.5t taper hatching barrels in advance, it is 1 cun of drainage switch that, which there is bore at cone bottom, with 200 Mesh filtering mesh bag or 200 mesh cotton bags filter nature seawater, and 80% water level is added in hatching barrel, then using bottom row's method removal Bad ovum and sundries in fertilized eggs;
(5) pond nursery
A, clear pond rich water
It is thoroughly cleaned up the pond with quick lime, apply fertilizer rich water, puts fertilized eggs after clear pond 10-15d, the 3-5d training before launching fertilized eggs Educate hydroplankton, in fine day, per acre press 1m water gage, splash rich water king 1-1.5kg, then apply viable bacteria 1kg and fermentation after Anchovy sauce 1kg, daytime starts aerator promotion phytoplankton growth after fertilising;
B, incubating oosperm
It is 3.0m × 3.0m × 1.3m or 3.0m × 4.0m × 1.3m cloth basket formula incubator, cloth that volume is built in pond It is blocked above basket with shading net, micro- inflation hatching;Hatching density is ten thousand/m of 8-123, in the item of 25-30 DEG C of water temperature, salinity 28 Under part, embryo passes through the development of 36-48h, hatches into prelarva;
C, seed is put in a suitable place to breed
The 2nd day after prelarva hatching, prelarva is put into pond from incubator, launches ten thousand tail of prelarva 10-12 per acre;
D, bait throwing in
Protozoan and wheel animalcule in the pool 1d-7d, 7d-12d artemia nauplii bait and Copepods cladocera, seedling after 13d Body, which has, looks for bait in water surface travelling, and initial stage starts to feed eel powder and hand fit's powder, when fry body is up to 2.5cm or more, Cooperation buoyancy feed can be fed, particle diameter can feed protozoan in the pool, wheel animalcule, artemia nauplii in 0.7mm, 7d The mixed fodder of bait and Copepods cladocera;
F, row changes water
Putting seedling initial stage does not change water, adds water 1% daily, changes water 10% daily after 14, feeds cooperation buoyancy feed;
E, daily management
The variation of observation water colour and seed activity condition daily, change water, oxygenation in time;Daily record water Inversion phenomenon, weather, A situation arises and disease control measure for the environmental factor and the activity condition of fish, situation of ingesting, health status, disease of stormy waves, Carry out cultivation log;
G, it harvests
After one month, fry overall length reaches 3.0cm, and juvenile fish metamorphosis can carry out intermediate rearing or directly cultivation when completing.
Preferably, the artificial fecundation method of the platax orbicularis, in step 2, parent fish rearing marine environment factor requirements:It is molten Solve oxygen > 4mg/L;pH7.8-8.4;Salinity 23-35;20-30 DEG C of water temperature;Transparency 60-80cm;Intensity of illumination >=3000Lx.By force Changing cultivation density is that 5.5-6.0m × 5.5-6.0m × 3.0-6.0m net cage puts parent population 100-200 tail in a suitable place to breed.
Preferably, the artificial fecundation method of the platax orbicularis, in step 2, count by weight percentage, artificial extruding is matched Close feed nutrition requirement:Crude protein content >=40%, crude fat >=6%, crude fibre≤4%, ash content≤14%, calcium 1-4%, always Phosphorus >=1.2%, lysine >=2.1%.
Preferably, the artificial fecundation method of the platax orbicularis, in step 3, chorion rush had both been can be used in oxytocic hormone Gonadal hormone HCG, ovulation induction No. 2 LRH-A2 of element and the plain No. 3 LRH-A3 one of which of ovulation induction, also can be used chorion rush property The hormotone of No. 3 LRH-A3 of glandular hormone HCG and ovulation induction No. 2 LRH-A2 of element or ovulation induction element;Wherein HCG injection dosage is 400-800U/kg, LRH-A2 or LRH-A3 are 4-6 μ g/kg, and male halves.
Preferably, the artificial fecundation method of the platax orbicularis, in step 3, oxytocic hormone injecting method is as follows:By HCG, LRH-A2 and LRH-A3 is dissolved in respectively in 0.6% sodium chloride solution of 100ML, then by HCG and LRH-A2 or HCG and LRH- Bis- kinds of solution mixing of A3, carry out intramuscular injection with single solution or mixed solution and hasten parturition.
Preferably, the artificial fecundation method of the platax orbicularis, in step 3, oviposition net cage and aquaculture net cage are 60-80 Mesh sieve tulles are made into, and oviposition net cage covers in aquaculture net cage.
Preferably, the artificial fecundation method of the platax orbicularis, in step 4, the washing methods of fertilized eggs:Fertilized eggs are used It receives ovum net to pull out, is collected into collection ovum net, then fertilized eggs are collected into 10kg breaker, the fertilized eggs in keg is poured into standby In the hatching barrel of 80% water, allows fertilized eggs to filter from the fishing net of 40 mesh, impurity is filtered;The screening technique of fertilized eggs: It is lightly stirred clockwise in hatching barrel with wooden dipper, rotates water in hatching barrel, until there is no fertilized eggs to sink to a barrel bottom, then allow Water is naturally static, then will accumulate in barrel bad ovum at bottom and sundries removal with bottom row's method, repeats this method 2-3 times, then with 100 mesh hands Diddle-net picks up fertilized eggs, is rinsed with fresh seawater.
Preferably, the artificial fecundation method of the platax orbicularis in step 4, collects the keed long 6m, Nang Kougao of ovum net 1.2m, distal opening diameter 0.4m, two sides respectively set long 6m wing lattice, high 1.2m, the high 0.9m in end among wing lattice.
In conclusion a kind of artificial propagation method of platax orbicularis of the invention has the beneficial effect that:
(1) platax orbicularis is the species of a great breeding prospect, and the present invention obtains the prelarva of batch by artificial propagation, To create conditions for the large-scale farming of platax orbicularis.
(2) present invention is that seawater fish artificial increasing cultivation increases new varieties, recovery to platax orbicularis natural resources and is supported Grow industrialized development play the role of it is extremely important.
Specific embodiment
Clear, complete description is carried out below with reference to technical effect of the embodiment to design and generation of the invention, with It is completely understood by the purpose of the present invention, feature and effect.Obviously, described embodiment is that a part of the invention is implemented Example, rather than whole embodiments, based on the embodiment of the present invention, those skilled in the art is not before making the creative labor Other embodiments obtained are put, the scope of protection of the invention is belonged to.
A kind of artificial fecundation method of platax orbicularis, includes the following steps:
(1) Juvenile stage
Parental source is the platax orbicularis of the capture of natural sea area or raising in cage, selects 4 more than age, weight 3.0-5.0kg, body Type is normal, disease-free, energetic, and the good platax orbicularis of gonad development is as parent;
(2) aquaculture net cage parent population reinforced cultivating
Select wind sheltering condition is good, water flow is unimpeded, depth of water 5m or more, sea area flow velocity less than 0.1m/s inner bay or shallow sea set Aquaculture net cage is set, 3 months progress parent population reinforced cultivatings before mating period;Artificial expanding compound feed is fed daily twice, upper, It in each 1 time of afternoon, is satiated with food and feeds, feed can add 0.2% fish oil, 0.5% multi-vitamins and 0.2% vitamin E, promote sexual gland Development;
(3) parent population is hastened parturition
It hastens parturition at sea to lay eggs and carry out in net cage, start April to progressively reach sexual gland maturation by the parent population of reinforced cultivating, It can carry out artificial induced spawning;Oxytocic hormone uses human chorionic gonadtropin HCG, ovulation induction No. 2 LRH-A2 of element and ovulation induction element 3 Number LRH-A3, injection oxytocic hormone are injected using dorsal muscles, are put into and are laid eggs in net cage after injection, through hormone induction 30-38h, allow it Naturally smart ovum, fertilization are discharged;
(4) collection and processing of fertilized eggs
After parent population oviposition, prepare 2 0.5t taper hatching barrels in advance, it is 1 cun of drainage switch that, which there is bore at cone bottom, with 200 Mesh filtering mesh bag or 200 mesh cotton bags filter nature seawater, and 80% water level is added in hatching barrel, then using bottom row's method removal Bad ovum and sundries in fertilized eggs;
(5) pond nursery
A, clear pond rich water
It is thoroughly cleaned up the pond with quick lime, apply fertilizer rich water, puts fertilized eggs after clear pond 10-15d, the 3-5d training before launching fertilized eggs Educate hydroplankton, in fine day, per acre press 1m water gage, splash rich water king 1-1.5kg, then apply viable bacteria 1kg and fermentation after Anchovy sauce 1kg, daytime starts aerator promotion phytoplankton growth after fertilising;
B, incubating oosperm
It is 3.0m × 3.0m × 1.3m or 3.0m × 4.0m × 1.3m cloth basket formula incubator, cloth that volume is built in pond It is blocked above basket with shading net, micro- inflation hatching;Hatching density is ten thousand/m of 8-123, in the item of 25-30 DEG C of water temperature, salinity 28 Under part, embryo passes through the development of 36-48h, hatches into prelarva;
C, seed is put in a suitable place to breed
The 2nd day after prelarva hatching, prelarva is put into pond from incubator, launches ten thousand tail of prelarva 10-12 per acre;
D, bait throwing in
Protozoan and wheel animalcule in the pool 1d-7d, 7d-12d artemia nauplii bait and Copepods cladocera, seedling after 13d Body, which has, looks for bait in water surface travelling, and initial stage starts to feed eel powder and hand fit's powder, when fry body is up to 2.5cm or more, Cooperation buoyancy feed can be fed, particle diameter can feed protozoan in the pool, wheel animalcule, artemia nauplii in 0.7mm, 7d The mixed fodder of bait and Copepods cladocera;
F, row changes water
Putting seedling initial stage does not change water, adds water 1% daily, changes water 10% daily after 14, feeds cooperation buoyancy feed;
E, daily management
The variation of observation water colour and seed activity condition daily, change water, oxygenation in time;Daily record water Inversion phenomenon, weather, A situation arises and disease control measure for the environmental factor and the activity condition of fish, situation of ingesting, health status, disease of stormy waves, Carry out cultivation log;
G, it harvests
After one month, fry overall length reaches 3.0cm, and juvenile fish metamorphosis can carry out intermediate rearing or directly cultivation when completing.
Embodiment 1-3, comparative example 1-3 are according to above-mentioned cultivation step, cultivation parameter shown in the following table 1, water area condition shown in table 2 It is cultivated, the results are shown in Table 3:
1 embodiment 1-3 of table, comparative example 1-3 cultivate parameter:
2 embodiment 1-3 of table, comparative example 1-3 water area condition:
Unit Embodiment 8 Embodiment 9 Embodiment 10 Comparative example 9 Comparative example 10 Comparative example 11
The depth of water m 6 5 7 4 3 4
Dissolved oxygen mg/L 4.3 4.5 4.6 3.9 3.6 3.8
Flow velocity m/s 0.09 0.08 0.08 0.11 0.09 0.12
PH —— 7.8-8.2 8.0-8.4 8.0-8.2 7.4-8.0 8.2-8.6 8.4-8.6
Salinity —— 28-35 23-30 25-31 20-23 34-40 28-34
Water temperature Degree Celsius 20-30 25-30 20-25 17-21 29-35 17-25
Transparency cm 70-80 60-70 60-70 50-60 80-90 60-70
Light intensity LX 3000 3200 3400 3000 2500 2800
3 embodiment 1-3 of table, comparative example 1-3 cultivate situation:
Unit Embodiment 1 Embodiment 2 Embodiment 3 Comparative example 1 Comparative example 2 Comparative example 3
Hatching density Ten thousand/M3 10 11 11.5 14 5 6
Embryonic Development Time h 36-38 40-48 36-42 50-61 49-57 58-64
Rate of embryonic death % 0.5 1.1 0.9 24 15 19
Fry culturing time D 30 28 33 40 38 45
The breeding process death rate % 1.2 0.8 0.7 10.5 12.4 11.7
From above-mentioned culture experiment result can be seen that according to given condition of the present invention can obtain rate of embryonic death it is lower, The breeding process death rate is low, and embryonic development is very fast, growth time is very fast, the more appropriate platax orbicularis fry of hatching density.Control Condition is unreasonable, then will lead to platax orbicularis rate of embryonic death and the breeding process death rate increases, in addition, embryonic Development Time and at Also can accordingly it extend for a long time.
Feed Ratio Experiments:
According to the given breeding environment of embodiment 3, change platax orbicularis feed formula such as table 4, designs following embodiment and comparison Example:
4 platax orbicularis forage compounding of table:
As can be seen that the speed of growth of platax orbicularis can be increased according to the given feed formula of the present invention.Wherein, comparative example 5 Insufficient on crude protein, total phosphorus, lysine, still, crude fibre, ash content etc. add excessively again, cause platax orbicularis growth slow Slowly.
The experiment of egg-collecting box size:
According to 3 culture environment of embodiment, 5 forage compounding of embodiment, according to 5 given condition of table design egg-collecting box shape and greatly Small, comparison of design example, other conditions are consistent.
The experiment of 5 egg-collecting box size of table:
Pass through above-mentioned experiment, it is found that according to institute of the invention to egg-collecting box shapes and sizes can obtain 11.5 ten thousand tails/ The hatching density of cubic meter, and the smaller 0.9% hatching death rate.The given egg-collecting box of comparative example 7 is too small, leads to hatching density Height, the death rate is larger, and comparative example 8 to egg-collecting box be open it is excessive, box volume is excessive, cause hatching density to decline, meanwhile, The death rate also increases.
Oxytocic hormone experiment:
According to 3 culture environment of embodiment, 5 forage compounding of embodiment, embodiment 7 give egg-collecting box design, other conditions protect It holds unanimously, designs embodiment 8-13, the results are shown in Table 6;Comparative example 9-14, the results are shown in Table 7:
The experiment of 6 embodiment 8-13 oxytocic hormone of table:
The experiment of 7 comparative example 9-14 oxytocic hormone of table:
Unit Comparative example 9 Comparative example 10 Comparative example 11 Comparative example 12 Comparative example 13 Comparative example 14
HCG U/KG 300 1000 300 0 0 0
LRH-A2 ug/kg 5 2 0 0 10 0
LRH-A3 ug/kg 5 0 0 10 5 3
Laying Ten thousand/M3 6 10 9 15 12 7
As can be seen that single use human chorionic gonadtropin HCG, ovulation induction No. 2 LRH-A2 of element and ovulation induction element 3 LRH-A3 is one of, or is swashed using human chorionic gonadtropin HCG and ovulation induction No. 2 LRH-A2 of element or chorionic gonadotrophin The hormotone of No. 3 LRH-A3 of plain HCG and ovulation induction element can obtain 180,000 every cubic metre or more of oviposition quantity.But It is, in single use human chorionic gonadtropin HCG, ovulation induction No. 2 LRH-A2 of element and the plain No. 3 LRH-A3 one of which of ovulation induction When, the too high or too low oviposition quantity that will lead to of dosage reduces;Quantity reduction of laying eggs can be made by being used in mixed way 3 kinds of oxytocic hormones also; In addition, using human chorionic gonadtropin HCG and ovulation induction element No. 2 LRH-A2 or human chorionic gonadtropin HCG and ovulation induction When the hormotone of No. 3 LRH-A3 of element, change the usage amount of a certain hormone, increasing or decreasing can all cause egg laying amount to decline.It is comprehensive It is upper described, it, should be in strict accordance with the usage amount of the given oxytocic hormone of the present invention when carrying out the injection of platax orbicularis oxytocic hormone.
Above-mentioned the embodiments of the present invention are described in detail, but present invention is not limited to the embodiments described above, Those skilled in the art within the scope of knowledge, can also make each without departing from the purpose of the present invention Kind variation.

Claims (8)

1. a kind of artificial fecundation method of platax orbicularis, which is characterized in that include the following steps:
(1) Juvenile stage
Parental source is that natural sea area captures or the platax orbicularis of raising in cage, select 4 more than age, weight 3.0-5.0kg, figure just Often, disease-free, energetic, the good platax orbicularis of gonad development is as parent;
(2) aquaculture net cage parent population reinforced cultivating
Select wind sheltering condition is good, water flow is unimpeded, depth of water 5m or more, sea area flow velocity less than 0.1m/s inner bay or shallow sea setting support Net cage is grown, 3 months progress parent population reinforced cultivatings before mating period;Artificial expanding compound feed is fed daily twice, the upper and lower noon It each 1 time, is satiated with food and feeds, 0.2% fish oil of feed addition, 0.5% multi-vitamins and 0.2% vitamin E promote gonad development;
(3) parent population is hastened parturition
It hastens parturition at sea to lay eggs and carry out in net cage, start April to progressively reach sexual gland maturation by the parent population of reinforced cultivating Carry out artificial induced spawning;Oxytocic hormone uses human chorionic gonadtropin HCG, ovulation induction No. 2 LRH-A2 of element and ovulation induction element 3 LRH-A3, injection oxytocic hormone are injected using dorsal muscles, are put into oviposition net cage after injection, through hormone induction 30-38h, allow its oneself So discharge smart ovum, fertilization;
(4) collection and processing of fertilized eggs
After parent population oviposition, prepare 2 0.5t taper hatching barrels in advance, it is 1 cun of drainage switch that, which there is bore at cone bottom, with 200 mesh mistakes Filter-screen bag or 200 mesh cotton bags filter nature seawater, and 80% water level is added in hatching barrel, then using bottom row's method removal fertilization Bad ovum and sundries in ovum;
(5) pond nursery
A, clear pond rich water
It is thoroughly cleaned up the pond with quick lime, apply fertilizer rich water, puts fertilized eggs after clear pond 10-15d, and the 3-5d before launching fertilized eggs cultivates water Middle planktonic organism presses 1m water gage in fine day per acre, and splash rich water king 1-1.5kg, then applies the anchovy sauce after viable bacteria 1kg and fermentation 1kg, daytime starts aerator promotion phytoplankton growth after fertilising;
B, incubating oosperm
Volume is built in pond for 3.0m × 3.0m × 1.3m or 3.0m × 4.0m × 1.3m cloth basket formula incubator, on cloth basket Side is blocked with shading net, micro- inflation hatching;Hatching density is ten thousand/m of 8-123, under conditions of 25-30 DEG C of water temperature, salinity 28, Embryo passes through the development of 36-48h, hatches into prelarva;
C, seed is put in a suitable place to breed
The 2nd day after prelarva hatching, prelarva is put into pond from incubator, launches ten thousand tail of prelarva 10-12 per acre;
D, bait throwing in
Protozoan and wheel animalcule in the pool 1d-7d, 7d-12d artemia nauplii bait and Copepods cladocera, seedling body has after 13d Bait is looked in water surface travelling, initial stage starts to feed eel powder and hand fit's powder, when fry body is up to 2.5cm or more, feeds Cooperate buoyancy feed, particle diameter is in 0.7mm;
F, row changes water
Putting seedling initial stage does not change water, adds water 1% daily, changes water 10% after 14 days daily, feeds cooperation buoyancy feed;
E, daily management
The variation of observation water colour and seed activity condition daily, change water, oxygenation in time;Record water Inversion phenomenon, weather, stormy waves daily Environmental factor and the activity condition of fish, situation of ingesting, health status, disease a situation arises and disease control measure, carry out Cultivate log;
G, it harvests
After January, fry overall length reaches 3.0cm, and juvenile fish metamorphosis can carry out intermediate rearing or directly cultivation when completing.
2. the artificial fecundation method of platax orbicularis according to claim 1, it is characterised in that:The step( 2)In, parent population Cultivate marine environment factor requirements:Dissolved oxygen > 4mg/L;pH7.8-8.4;Salinity 23-35;20-30 DEG C of water temperature;Transparency 60- 80cm;Intensity of illumination >=3000Lx;Reinforced cultivating density is that 5.5-6.0m × 5.5-6.0m × 3.0-6.0m net cage puts parent in a suitable place to breed Fish 100-200 tail.
3. the artificial fecundation method of platax orbicularis according to claim 1, it is characterised in that:The step( 2)In, by weight It measures percentage to calculate, artificial expanding compound feed nutritional requirement:Crude protein content >=40%, crude fat >=6%, crude fibre≤ 4%, ash content≤14%, calcium 1-4%, total phosphorus >=1.2%, lysine >=2.1%.
4. the artificial fecundation method of platax orbicularis according to claim 1, it is characterised in that:The step( 3)In, it hastens parturition Human chorionic gonadtropin HCG, ovulation induction No. 2 LRH-A2 of element and No. 3 LRH-A3 of ovulation induction element wherein one had both can be used in hormone Kind, the mixing of No. 3 LRH-A3 of human chorionic gonadtropin HCG and ovulation induction No. 2 LRH-A2 of element or ovulation induction element also can be used Hormone;It is 4-6 μ g/kg that wherein HCG injection dosage, which is 400-800U/kg, LRH-A2 or LRH-A3, and male halves.
5. the artificial fecundation method of platax orbicularis according to claim 1, it is characterised in that:The step( 3)In, oviposition Net cage is that 60-80 mesh sieve tulles are made into aquaculture net cage, and oviposition net cage covers in aquaculture net cage.
6. the artificial fecundation method of platax orbicularis according to claim 1, it is characterised in that:The step( 4)In, fertilization The washing methods of ovum:Fertilized eggs are pulled out with ovum net is received, are collected into collection ovum net, then fertilized eggs are collected into 10kg breaker It is interior, the fertilized eggs in keg are poured into the hatching barrel for getting 80% water ready, allow fertilized eggs to filter from the fishing net of 40 mesh, miscellaneous Matter filters;The screening technique of fertilized eggs:It is lightly stirred clockwise in hatching barrel with wooden dipper, rotates water in hatching barrel, until There is no fertilized eggs to sink to a barrel bottom, then allow water naturally static, then will accumulate in barrel bad ovum at bottom and sundries removal, weight with bottom row's method Multiple this method 2-3 times, then fertilized eggs are picked up with 100 mesh fishing nets, are rinsed with fresh seawater.
7. the artificial fecundation method of platax orbicularis according to claim 6, it is characterised in that:The step( 4)In, collect ovum The long 6m of the keed of net, the high 1.2m of capsule mouth, distal opening diameter 0.4m, two sides respectively set long 6m wing lattice, high 1.2m among wing lattice, end height 0.9m。
CN201710891104.XA 2017-09-27 2017-09-27 A kind of artificial fecundation method of platax orbicularis Active CN107581106B (en)

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Denomination of invention: Method of artificial propagation of platax orbicularis

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