CN107581106A - A kind of artificial fecundation method of platax orbicularis - Google Patents
A kind of artificial fecundation method of platax orbicularis Download PDFInfo
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Abstract
The invention discloses a kind of artificial fecundation method of platax orbicularis, comprise the following steps：1 Juvenile stage, the net cage reinforced cultivating of 2 parent populations, 3 parent populations are hastened parturition, the collection of 4 embryonated eggs and processing, 5 incubating oosperms, the pond rearing of 6 seeds.The present invention promotes Gunther parent fish spawning by the parent culture to platax orbicularis, then goes out prelarva with incubating oosperm, cultivates into that can support the fingerling grown, is that the large-scale farming of platax orbicularis creates conditions.Meanwhile the present invention adds new varieties for seawater fish artificial increasing cultivation, recovery and aquaculture industry development to platax orbicularis natural resources serve extremely important effect.
Description
Technical field
The present invention relates to technical field of aquaculture, and in particular to a kind of artificial fecundation method of platax orbicularis.
Background technology
Platax orbicularis (scientific name:Platax orbicularis) also known as circle eye Spanish mackerel, circle bat etc., it is Perciformes, pierces tail fish
Suborder, orbfish section, a kind of seawater fish in the life of torrid areas immediate offshore area of Spanish mackerel category, it is distributed in India-Western Pacific
Warm water waters, North gets South Japan, central Pacific island are arrived in south up to Australia, east, west to the eastern bank in Red sea and Africa, China
Originate in the South Sea.
Platax orbicularis lives in 5-30 rice depth, has the marine site that slightly mud is protected, haunts about near the accident shipwreck of deep-sea.
Juvenile fish is mainly inhabited in shallow sea or river mouth waters, because body colour is withered and yellow brown, meeting mimicry is hidden in floating on water into dead leaf shape
Under thing, often alone or groupuscule is moved about in lagoon inner bay.Adult fish then gos deep into less than about 30 meters of Coral Reef Region, on its peripheral slope
It is in pairs or movable in groups.The circle eye Spanish mackerel category day omnivorous fish of row, mainly using algae, invertebrate or small fish as
Food.Platax orbicularis juvenile fish fine figure, can be as ornamental fish；Adult fish then edible, delicious meat.
Due to excessive fishing so that the population quantity of platax orbicularis is greatly decreased, and the market supply is limited, can not meet city
The demand of field.At present, the artificial scale breeding technology of platax orbicularis, which has not yet to see, has been reported that.Artificial scale breeding technology not into
Ripe is to restrict it to propagate one of key factor of industry development artificially.
The content of the invention
Present invention aims at a kind of artificial fecundation method of platax orbicularis is provided, by the parent culture to platax orbicularis, promote
Make Gunther parent fish spawning, then go out prelarva with incubating oosperm, cultivate into that can support the fingerling grown, be that large-scale cultivation platax orbicularis is established
Basis.
A kind of artificial fecundation method of platax orbicularis, comprises the following steps：
1 Juvenile stage
Parental source is the platax orbicularis of the capture of natural sea area or raising in cage, selects 4 more than age, body weight 3.0-5.0kg, body
Type is normal, disease-free, energetic, and the good platax orbicularis of gonad development is as parent；
2 aquaculture net cage parent population reinforced cultivatings
Select wind sheltering condition is good, current are unimpeded, more than depth of water 5m, marine site flow velocity less than 0.1m/s inner bay or shallow sea set
Aquaculture net cage is put, carries out parent population reinforced cultivating within 3 months before mating period；Artificial expanding compound feed is fed daily twice, upper,
In each 1 time of afternoon, it is satiated with food and feeds, feed can adds 0.2% fish oil, 0.5% B B-complex and 0.2% vitamin E, promote sexual gland
Development；
3 parent populations are hastened parturition
Hasten parturition at sea to lay eggs and carry out in net cage, start April to progressively reach sexual gland maturation by the parent population of reinforced cultivating,
Artificial induced spawning can be carried out；Oxytocic hormone uses human chorionic gonadtropin HCG, ovulation induction No. 2 LRH-A2 of element and ovulation induction element 3
Number LRH-A3, injection oxytocic hormone are injected using dorsal muscles, are put into and are laid eggs in net cage after injection, through hormone induction about 30-38h, are allowed
It discharges smart ovum, fertilization naturally；
The collection and processing of 4 embryonated eggs
After Gunther parent fish spawning, prepare 2 0.5t taper hatching barrels in advance, there is 1 cun of drainage switch at cone bottom, filtered with 200 mesh
Mesh bag or 200 mesh cotton bags filtering nature seawater, 80% water level is added in hatching barrel, embryonated egg is then removed using bottom row's method
In bad ovum and debris；
5 pond nursery
A, clear pond rich water
Thoroughly cleaned up the pond with quick lime, applied fertilizer rich water, and embryonated egg is put after clear pond 10-15d, the 3-5d trainings before embryonated egg is launched
Educate hydroplankton, in every mu of fine day, by 1m water gages, splash rich water king 1-1.5kg, then apply viable bacteria 1kg and fermentation after
Anchovy sauce 1kg, after fertilising daytime start aerator promote phytoplankton growth；
B, incubating oosperm
The cloth basket formula incubator that volume is 3.0m × 3.0m × 1.3m or 3.0m × 4.0m × 1.3m, cloth are built in pond
Blocked above basket with shade net, micro- inflation hatching；Hatching density is ten thousand/m3 of 8-12, in the bar of 25-30 DEG C of water temperature, salinity 28
Under part, embryo passes through 36-48h development, hatches into prelarva；
C, seed is put in a suitable place to breed
The 2nd day after prelarva hatches, prelarva is put into pond from incubator, every mu is launched the tails of prelarva 10-12 ten thousand；
D, bait throwing in
Protozoan and wheel animalcule in the 1d-7d pools, 7d-12d artemia naupliis bait and Copepods cladocera, seedling after 13d
Body, which has to move about in the water surface, looks for bait, starts initial stage to feed eel powder and hand fit's powder, when fry body is up to more than 2.5cm,
It can feed and coordinate buoyancy feed, particle diameter is in 0.7mm；
F, row changes water
Put seedling and do not change water initial stage, add water 1% daily, water 10% is changed daily after 14, feed and coordinate buoyancy feed；
E, daily management
The change of observation water colour and seed active situation daily, change water, oxygenation in time；Daily record water Inversion phenomenon, weather,
A situation arises and disease control measure for the envirment factors such as stormy waves and the active situation of fish, situation of ingesting, health status, disease
Deng carrying out cultivation daily record；
G, harvest
After one month, fry total length reaches 3.0cm, and juvenile fish metamorphosis can carry out intermediate rearing or directly cultivation when completing.
Preferably, the artificial fecundation method of described platax orbicularis, in step 2, parent fish rearing marine environment factor requirements：It is molten
Solve oxygen ＞ 4mg/L；pH7.8-8.4；Salinity 23-35；20-30 DEG C of water temperature；Transparency 60-80cm；Intensity of illumination >=3000Lx.By force
Change the net cage that cultivation density is 5.5-6.0m × 5.5-6.0m × 3.0-6.0m and put parent population 100-200 tails in a suitable place to breed.
Preferably, the artificial fecundation method of described platax orbicularis, it is count by weight percentage, artificial expanded to match somebody with somebody in step 2
Close feed request nutritional requirement：Crude protein content >=40%, crude fat >=6%, crude fibre≤4%, ash content≤14%, calcium 1-
4%, total phosphorus >=1.2%, lysine >=2.1%.
Preferably, the artificial fecundation method of described platax orbicularis, in step 3, oxytocic hormone can both be promoted using chorion
Gonadal hormone HCG, ovulation induction No. 2 LRH-A2 of element and the plain No. 3 LRH-A3 one of which of ovulation induction, can also use chorion rush property
No. 3 LRH-A3 of glandular hormone HCG and ovulation induction No. 2 LRH-A2 of element or ovulation induction element hormotone；Wherein HCG injections dosage is
400-800U/kg, LRH-A2 or LRH-A3 are 4-6 μ g/kg, and male halves.
Preferably, the artificial fecundation method of described platax orbicularis, in step 3, oxytocic hormone injecting method is as follows：By HCG,
LRH-A2 and LRH-A3 is dissolved in the sodium chloride solutions of 100ML 0.6% respectively, then by HCG and LRH-A2 or HCG and LRH-
Bis- kinds of solution mixing of A3, carry out intramuscular injection with single solution or mixed solution and hasten parturition.
Preferably, the artificial fecundation method of described platax orbicularis, in step 3, spawning net cage and aquaculture net cage are 60-80
Mesh sieve tulles are made, and spawning net cage is enclosed in aquaculture net cage.
Preferably, the artificial fecundation method of described platax orbicularis, in step 4, the washing methods of embryonated egg：Embryonated egg is used
Receive ovum net to pull out, be collected into collection ovum net, then embryonated egg is collected into 10kg breakers, the embryonated egg in keg is poured into standby
In the hatching barrel of 80% water, allow embryonated egg to be filtered from the fishing net of 40 mesh, impurity is filtered；The screening technique of embryonated egg：
Lightly stirred clockwise in hatching barrel with wooden dipper, water is rotated in hatching barrel, until no embryonated egg sinks to a barrel bottom, Ran Hourang
Water is naturally static, then will accumulate in barrel bad ovum at bottom and debris removal with bottom row's method, this method is repeated 2-3 times, then with 100 mesh hands
Diddle-net picks up embryonated egg, is rinsed with fresh seawater.
Preferably, the artificial fecundation method of described platax orbicularis, in step 4, the keed long 6m, Nang Kougao of ovum net are collected
1.2m, distal opening footpath about 0.4m, both sides respectively set long 6m wing lattices, wing lattice centre high 1.2m, the high 0.9m in end.
In summary, a kind of of the invention artificial propagation method of platax orbicularis has the beneficial effect that：
(1) platax orbicularis is the species of a great breeding prospect, and the present invention obtains the prelarva of batch by artificial propagation,
So as to be created conditions for the large-scale farming of platax orbicularis.
(2) present invention adds new varieties for seawater fish artificial increasing cultivation, recovery to platax orbicularis natural resources and supports
Grow industrialized development and serve extremely important effect.
Embodiment
Clear, complete description is carried out below with reference to design of the embodiment to the present invention and caused technique effect, with
It is completely understood by the purpose of the present invention, feature and effect.Obviously, described embodiment is the part implementation of the present invention
Example, rather than whole embodiments, based on embodiments of the invention, those skilled in the art is not before creative work is paid
Obtained other embodiment is put, belongs to the scope of protection of the invention.
A kind of artificial fecundation method of platax orbicularis, comprises the following steps：
1 Juvenile stage
Parental source is the platax orbicularis of the capture of natural sea area or raising in cage, selects 4 more than age, body weight 3.0-5.0kg, body
Type is normal, disease-free, energetic, and the good platax orbicularis of gonad development is as parent；
2 aquaculture net cage parent population reinforced cultivatings
Select wind sheltering condition is good, current are unimpeded, more than depth of water 5m, marine site flow velocity less than 0.1m/s inner bay or shallow sea set
Aquaculture net cage is put, carries out parent population reinforced cultivating within 3 months before mating period；Artificial expanding compound feed is fed daily twice, upper,
In each 1 time of afternoon, it is satiated with food and feeds, feed can adds 0.2% fish oil, 0.5% B B-complex and 0.2% vitamin E, promote sexual gland
Development；
3 parent populations are hastened parturition
Hasten parturition at sea to lay eggs and carry out in net cage, start April to progressively reach sexual gland maturation by the parent population of reinforced cultivating,
Artificial induced spawning can be carried out；Oxytocic hormone uses human chorionic gonadtropin HCG, ovulation induction No. 2 LRH-A2 of element and ovulation induction element 3
Number LRH-A3, injection oxytocic hormone are injected using dorsal muscles, are put into and are laid eggs in net cage after injection, through hormone induction about 30-38h, are allowed
It discharges smart ovum, fertilization naturally；
The collection and processing of 4 embryonated eggs
After Gunther parent fish spawning, prepare 2 0.5t taper hatching barrels in advance, there is 1 cun of drainage switch at cone bottom, filtered with 200 mesh
Mesh bag or 200 mesh cotton bags filtering nature seawater, 80% water level is added in hatching barrel, embryonated egg is then removed using bottom row's method
In bad ovum and debris；
5 pond nursery
A, clear pond rich water
Thoroughly cleaned up the pond with quick lime, applied fertilizer rich water, and embryonated egg is put after clear pond 10-15d, the 3-5d trainings before embryonated egg is launched
Educate hydroplankton, in every mu of fine day, by 1m water gages, splash rich water king 1-1.5kg, then apply viable bacteria 1kg and fermentation after
Anchovy sauce 1kg, after fertilising daytime start aerator promote phytoplankton growth；
B, incubating oosperm
The cloth basket formula incubator that volume is 3.0m × 3.0m × 1.3m or 3.0m × 4.0m × 1.3m, cloth are built in pond
Blocked above basket with shade net, micro- inflation hatching；Hatching density is ten thousand/m3 of 8-12, in the bar of 25-30 DEG C of water temperature, salinity 28
Under part, embryo passes through 36-48h development, hatches into prelarva；
C, seed is put in a suitable place to breed
The 2nd day after prelarva hatches, prelarva is put into pond from incubator, every mu is launched the tails of prelarva 10-12 ten thousand；
D, bait throwing in
Protozoan and wheel animalcule in the 1d-7d pools, 7d-12d artemia naupliis bait and Copepods cladocera, seedling after 13d
Body, which has to move about in the water surface, looks for bait, starts initial stage to feed eel powder and hand fit's powder, when fry body is up to more than 2.5cm,
It can feed and coordinate buoyancy feed, particle diameter is in 0.7mm；
F, row changes water
Put seedling and do not change water initial stage, add water 1% daily, water 10% is changed daily after 14, feed and coordinate buoyancy feed；
E, daily management
The change of observation water colour and seed active situation daily, change water, oxygenation in time；Daily record water Inversion phenomenon, weather,
A situation arises and disease control measure for the envirment factors such as stormy waves and the active situation of fish, situation of ingesting, health status, disease
Deng carrying out cultivation daily record；
G, harvest
After one month, fry total length reaches 3.0cm, and juvenile fish metamorphosis can carry out intermediate rearing or directly cultivation when completing.
Embodiment 1-3, comparative example 1-3 are according to above-mentioned cultivation step, cultivation parameter shown in table 1 below, water area condition shown in table 2
Cultivated, its result is as shown in table 3：
The embodiment 1-3 of table 1, comparative example 1-3 cultivate parameter：
The embodiment 1-3 of table 2, comparative example 1-3 water area conditions：
| Unit | Embodiment 8 | Embodiment 9 | Embodiment 10 | Comparative example 9 | Comparative example 10 | Comparative example 11 | |
| The depth of water | m | 6 | 5 | 7 | 4 | 3 | 4 |
| Dissolved oxygen | mg/L | 4.3 | 4.5 | 4.6 | 3.9 | 3.6 | 3.8 |
| Flow velocity | m/s | 0.09 | 0.08 | 0.08 | 0.11 | 0.09 | 0.12 |
| PH | —— | 7.8-8.2 | 8.0-8.4 | 8.0-8.2 | 7.4-8.0 | 8.2-8.6 | 8.4-8.6 |
| Salinity | —— | 28-35 | 23-30 | 25-31 | 20-23 | 34-40 | 28-34 |
| Water temperature | Degree Celsius | 20-30 | 25-30 | 20-25 | 17-21 | 29-35 | 17-25 |
| Transparency | cm | 70-80 | 60-70 | 60-70 | 50-60 | 80-90 | 60-70 |
| Light intensity | LX | 3000 | 3200 | 3400 | 3000 | 2500 | 2800 |
The embodiment 1-3 of table 3, comparative example 1-3 cultivate situation：
| Unit | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 | |
| Hatching density | Ten thousand/M3 | 10 | 11 | 11.5 | 14 | 5 | 6 |
| Embryonic Development Time | h | 36-38 | 40-48 | 36-42 | 50-61 | 49-57 | 58-64 |
| Rate of embryonic death | % | 0.5 | 1.1 | 0.9 | 24 | 15 | 19 |
| Fry culturing time | D | 30 | 28 | 33 | 40 | 38 | 45 |
| The breeding process death rate | % | 1.2 | 0.8 | 0.7 | 10.5 | 12.4 | 11.7 |
From above-mentioned culture experiment result can be seen that according to given condition of the present invention can obtain rate of embryonic death it is relatively low,
The breeding process death rate is low, and embryonic development is very fast, growth time is very fast, the more suitable platax orbicularis fry of hatching density.Control
Condition is unreasonable, then platax orbicularis rate of embryonic death and the breeding process death rate can be caused to increase, in addition, embryonic Development Time and into
Also can accordingly it extend for a long time.
Feed Ratio Experiments：
Breeding environment is given according to embodiment 3, changes platax orbicularis feed formula such as table 4, designs following examples and contrast
Example：
The platax orbicularis forage compounding of table 4：
As can be seen that giving feed formula according to the present invention, the speed of growth of platax orbicularis can be increased.Wherein, comparative example 5
Insufficient on crude protein, total phosphorus, lysine, still, crude fibre, ash grade and added excessively, cause platax orbicularis growth slow
Slowly.
Egg-collecting box size is tested：
According to the culture environment of embodiment 3, the forage compounding of embodiment 5, according to the given condition of table 5 design egg-collecting box shape and greatly
Small, comparison of design example, other conditions are consistent.
The egg-collecting box size of table 5 is tested：
Pass through above-mentioned experiment, it is found that according to institute of the invention to egg-collecting box shapes and sizes can obtain 11.5 ten thousand tails/
The hatching density of cubic meter, and the smaller 0.9% hatching death rate.Comparative example 7 gives egg-collecting box too small, causes hatching density
Height, the death rate is larger, and comparative example 8 it is excessive to egg-collecting box opening, box volume is excessive, causes hatching density to decline, meanwhile,
The death rate also increases.
Oxytocic hormone is tested：
According to the culture environment of embodiment 3, the forage compounding of embodiment 5, embodiment 7 give egg-collecting box design, other conditions protect
Consistent, design embodiments 8-13 is held, its result is as shown in table 6；Comparative example 9-14, its result are as shown in table 7：
The embodiment 8-13 oxytocic hormones of table 6 are tested：
The comparative example 9-14 oxytocic hormones of table 7 are tested：
| Unit | Comparative example 9 | Comparative example 10 | Comparative example 11 | Comparative example 12 | Comparative example 13 | Comparative example 14 | |
| HCG | U/KG | 300 | 1000 | 300 | 0 | 0 | 0 |
| LRH-A2 | ug/kg | 5 | 2 | 0 | 0 | 10 | 0 |
| LRH-A3 | ug/kg | 5 | 0 | 0 | 10 | 5 | 3 |
| Laying | Ten thousand/M3 | 6 | 10 | 9 | 15 | 12 | 7 |
As can be seen that single use human chorionic gonadtropin HCG, ovulation induction No. 2 LRH-A2 of element and ovulation induction element 3
LRH-A3 one of which, or swashed using human chorionic gonadtropin HCG and ovulation induction No. 2 LRH-A2 of element or chorionic gonadotrophin
No. 3 LRH-A3 of plain HCG and ovulation induction element hormotone can obtain more than 180,000 every cubic metre of spawning quantity.But
It is, in single use human chorionic gonadtropin HCG, ovulation induction No. 2 LRH-A2 of element and the plain No. 3 LRH-A3 one of which of ovulation induction
When, dosage is too high or too low to cause quantity reduction of laying eggs；Quantity reduction of laying eggs can also be caused by being used in mixed way 3 kinds of oxytocic hormones；
In addition, use human chorionic gonadtropin HCG and ovulation induction No. 2 LRH-A2 of element or human chorionic gonadtropin HCG and ovulation induction
During No. 3 LRH-A3 of element hormotone, change the usage amount of a certain hormone, increasing or decreasing can all cause egg laying amount to decline.It is comprehensive
It is upper described, when carrying out the injection of platax orbicularis oxytocic hormone, the usage amount of oxytocic hormone should be given in strict accordance with the present invention.
It is above-mentioned that embodiments of the present invention are explained in detail, but the present invention is not limited to above-mentioned embodiment,
In one of ordinary skill in the art's possessed knowledge, it can also be made on the premise of present inventive concept is not departed from each
Kind change.
Claims (7)
1. a kind of artificial fecundation method of platax orbicularis, it is characterised in that comprise the following steps：
（1）Juvenile stage
Parental source is that natural sea area captures or the platax orbicularis of raising in cage, select 4 more than age, body weight 3.0-5.0kg, build just
Often, disease-free, energetic, the good platax orbicularis of gonad development is as parent；
（2）Aquaculture net cage parent population reinforced cultivating
Select wind sheltering condition is good, current are unimpeded, more than depth of water 5m, marine site flow velocity less than 0.1m/s inner bay or shallow sea set support
Net cage is grown, carries out parent population reinforced cultivating within 3 months before mating period；Artificial expanding compound feed is fed daily twice, the upper and lower noon
Each 1 time, it is satiated with food and feeds, feed can adds 0.2% fish oil, 0.5% B B-complex and 0.2% vitamin E, promote sexual gland hair
Educate；
（3）Parent population is hastened parturition
Hasten parturition at sea to lay eggs and carry out in net cage, start April to progressively reach sexual gland maturation by the parent population of reinforced cultivating, you can
Carry out artificial induced spawning；Oxytocic hormone uses human chorionic gonadtropin HCG, ovulation induction No. 2 LRH-A2 of element and ovulation induction element 3
LRH-A3, injection oxytocic hormone are injected using dorsal muscles, are put into after injection in spawning net cage, through hormone induction about 30-38h, are allowed it
Naturally smart ovum, fertilization are discharged；
（4）The collection and processing of embryonated egg
After Gunther parent fish spawning, prepare 2 0.5t taper hatching barrels in advance, there is 1 cun of drainage switch at cone bottom, with 200 mesh filtering mesh bags
Or 200 mesh cotton bag filtering nature seawater, in hatching barrel plus 80% water level, then using bottom row method remove embryonated egg in
Bad ovum and debris；
（5）Pond nursery
A, clear pond rich water
Thoroughly cleaned up the pond with quick lime, applied fertilizer rich water, and embryonated egg is put after clear pond 10-15d, and the 3-5d before embryonated egg is launched cultivates water
Middle planktonic organism, in every mu of fine day, by 1m water gages, splash rich water king 1-1.5kg, then applies the anchovy sauce after viable bacteria 1kg and fermentation
1kg, after fertilising daytime start aerator promote phytoplankton growth；
B, incubating oosperm
The cloth basket formula incubator that volume is 3.0m × 3.0m × 1.3m or 3.0m × 4.0m × 1.3m is built in pond, on cloth basket
Side is blocked with shade net, micro- inflation hatching；Hatching density is ten thousand/m3 of 8-12, under conditions of 25-30 DEG C of water temperature, salinity 28,
Embryo passes through 36-48h development, hatches into prelarva；
C, seed is put in a suitable place to breed
The 2nd day after prelarva hatches, prelarva is put into pond from incubator, every mu is launched the tails of prelarva 10-12 ten thousand；
D, bait throwing in
Protozoan and wheel animalcule in the 1d-7d pools, 7d-12d artemia naupliis bait and Copepods cladocera, seedling body has after 13d
Moved about in the water surface and look for bait, started initial stage to feed eel powder and hand fit's powder, when fry body is up to more than 2.5cm, feed
Coordinate buoyancy feed, particle diameter is in 0.7mm；
F, row changes water
Put seedling and do not change water initial stage, add water 1% daily, water 10% is changed daily after 14, feed and coordinate buoyancy feed；
E, daily management
The change of observation water colour and seed active situation daily, change water, oxygenation in time；Record water Inversion phenomenon, weather, stormy waves daily
Etc. envirment factor and the active situation of fish, situation of ingesting, health status, disease, a situation arises and disease control measure etc., does
Cultivate daily record well；
G, harvest
After January, fry total length reaches 3.0cm, and juvenile fish metamorphosis can carry out intermediate rearing or directly cultivation when completing.
2. the artificial fecundation method of platax orbicularis according to claim 1, it is characterised in that：In the step 2, parent fish rearing
Marine environment factor requirements：Dissolved oxygen ＞ 4mg/L；pH7.8-8.4；Salinity 23-35；20-30 DEG C of water temperature；Transparency 60-80cm；
Intensity of illumination >=3000Lx.Reinforced cultivating density is that 5.5-6.0m × 5.5-6.0m × 3.0-6.0m net cage puts parent population 100- in a suitable place to breed
200 tails.
3. the artificial fecundation method of platax orbicularis according to claim 1, it is characterised in that：In the step 2, by weight hundred
Divide than calculating, artificial expanding compound feed requires nutritional requirement：Crude protein content >=40%, crude fat >=6%, crude fibre≤
4%th, ash content≤14%, calcium 1-4%, total phosphorus >=1.2%, lysine >=2.1%.
4. the artificial fecundation method of platax orbicularis according to claim 1, it is characterised in that：In the step 3, oxytocic hormone
Both human chorionic gonadtropin HCG, ovulation induction No. 2 LRH-A2 of element and the plain No. 3 LRH-A3 one of which of ovulation induction can have been used,
No. 3 LRH-A3 of human chorionic gonadtropin HCG and ovulation induction No. 2 LRH-A2 of element or ovulation induction element hormotone can be used；
Wherein HCG injections dosage is that 400-800U/kg, LRH-A2 or LRH-A3 are 4-6 μ g/kg, and male halves.
5. the artificial fecundation method of platax orbicularis according to claim 1, it is characterised in that：In the step 3, net cage of laying eggs
It is that 60-80 mesh sieve tulles are made with aquaculture net cage, spawning net cage is enclosed in aquaculture net cage.
6. the artificial fecundation method of platax orbicularis according to claim 1, it is characterised in that：In the step 4, embryonated egg
Washing methods：Embryonated egg is pulled out with ovum net is received, is collected into collection ovum net, then embryonated egg is collected into 10kg breakers, will
Embryonated egg in keg is poured into the hatching barrel for getting 80% water ready, allows embryonated egg to be filtered from the fishing net of 40 mesh, impurity is filtered
Fall；The screening technique of embryonated egg：Lightly stirred clockwise in hatching barrel with wooden dipper, water is rotated in hatching barrel, until not having
Embryonated egg sinks to a barrel bottom, then allows water naturally static, then will accumulate in barrel bad ovum at bottom and debris removal with bottom row's method, repeats this
Method 2-3 times, then embryonated egg is picked up with 100 mesh fishing nets, rinsed with fresh seawater.
7. the artificial fecundation method of platax orbicularis according to claim 1, it is characterised in that：In the step 4, collection ovum net
The long 6m of keed, the high 1.2m of capsule mouth, distal opening footpath about 0.4m, both sides respectively set long 6m wing lattices, the high 1.2m in wing lattice centre, end height
0.9m。
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| CN108770741A (en) * | 2018-05-23 | 2018-11-09 | 海南晨海水产有限公司 | A kind of hatching method of east star spot |
| CN109220903A (en) * | 2018-09-26 | 2019-01-18 | 中国水产科学研究院南海水产研究所 | A kind of point wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method |
| CN109548708A (en) * | 2018-10-11 | 2019-04-02 | 中国水产科学研究院南海水产研究所 | A kind of point wing Spanish mackerel batch production offspring seed cultivation method |
| CN111109157A (en) * | 2019-11-22 | 2020-05-08 | 中国水产科学研究院南海水产研究所 | Artificial spawning induction method and agent for micropterus salmoides |
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| CN113303255A (en) * | 2021-06-23 | 2021-08-27 | 中国水产科学研究院东海水产研究所 | Method for artificially cultivating siganus longipes parents for large-scale natural propagation |
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Denomination of invention: Method of artificial propagation of platax orbicularis Effective date of registration: 20200616 Granted publication date: 20181130 Pledgee: Agricultural Development Bank of China Lingshui Li Autonomous County sub branch Pledgor: HAINAN CHENHAI AQUATIC Co.,Ltd. Registration number: Y2020980003102 |