CN103461253B - Yellow River fish artificial fecundation method - Google Patents
Yellow River fish artificial fecundation method Download PDFInfo
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- CN103461253B CN103461253B CN201310449845.4A CN201310449845A CN103461253B CN 103461253 B CN103461253 B CN 103461253B CN 201310449845 A CN201310449845 A CN 201310449845A CN 103461253 B CN103461253 B CN 103461253B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A20/00—Water conservation; Efficient water supply; Efficient water use
- Y02A20/40—Protecting water resources
- Y02A20/402—River restoration
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
Yellow River fish artificial fecundation method of the present invention, comprises parent population selection, parent population cultivation, artificial induced spawning, hatching, fry rearing.Spawning rate of the present invention, incubation rate be high, can have efficient recovery the upper reaches of the Yellow River Yellow River fish resource.
Description
technical field:
What the present invention relates to is a kind of artificial propagation of fish method, particularly be a kind of Yellow River fish artificial fecundation method.
background technology:
Yellow River fish (Triplophysasiluroides) belongs to Cypriniformes, Cyprinidae, Schizopygopsis, is commonly called as: smallmouth Gymnocypris przewalskii, little face fish.Yellow River fish be a kind of inhabit highlands the upper reaches of the Yellow River Heavenly Stems and Earthly Branches stream and lake and Qaidam water system, be producing region Main Commercial Fishes.Hide when surviving the winter among cave, riverbank or rock crevice, like limpid cold water.Distribution height above sea level is everlasting 2000-4500m highly.Based on feeding plant food, often with the cutin edge of lower jaw prosperity scraping attached algae and submerged plant chip at the bottom of grit surface or mud, hold concurrently food part aquatic vascular plant blade and aquatic insect.Because cruel fishing is excessively captured and the destruction of ecotope, result causes resource significantly to decline, and artificial propagation Yellow River fish has become a kind of inevitable.
The at present report of relevant Yellow River fish proliferation, fisheries environment monitoring station, rarely seen Qinghai Province collected wild mature brood fish in 2009 on limit, the Yellow River, and carry out the relevant report of ovum artificial collection, hatching and embryonic development, the research of artificial propagation aspect also belongs to blank.
summary of the invention:
The object that the present invention intends providing is to provide a kind of method that spawning rate, incubation rate are high, can have the artificial propagation Yellow River fish of efficient recovery the upper reaches of the Yellow River Yellow River fish resource.
The object of the present invention is achieved like this:
Yellow River fish artificial fecundation method of the present invention, comprises parent population selection, parent population cultivation, artificial induced spawning, hatching, fry rearing.
The present invention includes following concrete steps:
(1) parent population is selected: the Yellow River mainstream and female milter more than tributary 5 age, and artificial domestication is cultivated after 2 ~ 3 years and hastened parturition;
(2) parent population is cultivated: select crude protein content to be at least 38% manufactured feed and throw something and feed, and within antenatal 3 months, need strengthen cultivating: selection water quality is good, water velocity is comparatively large, water temperature is cultivated in the cold water environment of 0 ~ 20 DEG C, antenatal 15 ~ 20 days injection LRH-A
20.2 μ g accelerates the ripening, and gives the stimulation by running water of 0.1 ~ 0.2m/s every day;
(3) artificial induced spawning: for the raun that maturity is good, water temperature can be hastened parturition 7 ~ 12 DEG C time, and oxytocic drug and consumption are: LRH-A
2(luteotropin releasing hormone d-ala analog) every kg body weight is 3 ~ 6 μ g; HCG(human chorionic gonadtropin) every kg body weight is 1000 ~ 1500IU, point 2 injections, first time injection is accumulated dose 1/8 ~ 1/6, the all dosage that 2nd pin injection is remaining, milter all adopts disposable injection, and dosage is 1/2 of raun accumulated dose, adopts dry method of artificial fertilization;
(4) hatch: adopt net cage hatching or Lotic hatching: adopt net cage hatching, at least 5cm placed under water by net cage, Spawning water is Natural Water, dissolved oxygen >=5mg/L, hatching water temperature 10 ~ 19 DEG C, pH value is 7.2 ~ 8.0, adopts Lotic hatching, bad ovum should be selected in time in hatching, ovum of not inseminating, dead ovum, incubator box will avoid illumination;
(5) fry rearing: maintain aforementioned hatching process until prelarva yolk sac absorbs complete, yolk sac should throw something and feed halogen worm or microzooplankton till flat trip after disappearing, fry rearing water temperature 15-20 DEG C.
The specific practice of above-mentioned employing dry method of artificial fertilization is: after the raun dry towel of maturation is dried body surface water, then ovum engagement is squeezed, clamp-on seminal fluid simultaneously, stir 2 ~ 3min with feather, sperm is fully contacted with ovum, now adds the clear water 500ml identical with Spawning water again, activate sperm and ovum, after continuing to stir 1min, add the unnecessary seminal fluid of 500ml clear water rinsed clean and foreign material, pour incubator box hatching into.When carrying out artificial insemination, should note avoiding sunlight direct irradiation sperm and ovum.
Artificial fecundation method fertilization rate of the present invention, incubation rate and or/motility rate high, fertilization rate can reach more than 90%, and incubation rate and survival rate can reach more than 80%, can have efficient recovery the upper reaches of the Yellow River Yellow River fish resource.
embodiment:
The present embodiment Yellow River fish artificial fecundation method, comprises parent population selection, parent population cultivation, artificial induced spawning, hatching, fry rearing five steps.Concrete steps are as follows:
(1) parent population is selected:
Select the female milter in the Yellow River mainstream and tributary 5 age more than, artificial domestication is cultivated after 2 ~ 3 years and is hastened parturition;
(2) parent population is cultivated:
Select crude protein content to throw something and feed at 38% manufactured feed, within antenatal 3 months, need strengthen cultivating.Feeding environment selection water quality is good, water velocity is comparatively large, water temperature is cultivated in the cold water environment of 0 ~ 20 DEG C, antenatal 15 ~ 20 days injection LRH-A
20.2 μ g accelerates the ripening, and gives the stimulation by running water of 0.1 ~ 0.2m/s every day;
(3) artificial induced spawning:
Take 2 pin injections to hasten parturition: for the raun that maturity is good, water temperature can be hastened parturition 7 ~ 12 DEG C time, and oxytocic drug and consumption are: LRH-A
2every kg body weight is the every kg body weight of 3 ~ 6 μ g, HCG is that 1000 ~ 1500IU is used in combination; Divide 2 injections, first time injects all dosage being that the 1/8 ~ 1/6,2nd pin injection of accumulated dose is remaining; Milter all adopts disposable injection, and dosage is 1/2 of raun accumulated dose;
Adopt dry method of artificial fertilization, specific practice is: after being dried by the raun body surface water of maturation, squeezes ovum engagement, clamp-on seminal fluid simultaneously, stir 2 ~ 3min with feather, sperm is fully contacted with ovum, now adds the clear water 500mL identical with Spawning water again, activate sperm and ovum, after continuing to stir 1min, add the unnecessary seminal fluid of 500mL clear water rinsed clean and foreign material, pour incubator box hatching into, when carrying out artificial insemination, should note avoiding sunlight direct irradiation sperm and ovum;
(4) hatch: adopt net cage hatching or Lotic hatching:
Employing net cage is hatched, and 5cm under water placed by net cage, and Spawning water is Natural Water, dissolved oxygen >=5mg/L, hatching water temperature about 15 DEG C, and pH value is 7.5;
Adopt Lotic hatching, in hatching, bad ovum should be selected in time, ovum of not inseminating, dead ovum, incubator box will avoid illumination;
(5) fry rearing:
Maintain aforementioned hatching process until prelarva yolk sac absorbs complete, yolk sac should throw something and feed halogen worm or microzooplankton till flat trip after disappearing, fry rearing water temperature 15-20 DEG C.
Above-described embodiment is further described foregoing of the present invention, but this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to above-described embodiment.All technology realized based on foregoing all belong to scope of the present invention.
Claims (2)
1. Yellow River fish artificial fecundation method, comprises parent population selection, parent population cultivation, artificial induced spawning, hatching, fry rearing, specifically comprises the following steps:
(1) parent population is selected: the Yellow River mainstream and female milter more than tributary 5 age, and artificial domestication is cultivated after 2 ~ 3 years and hastened parturition;
(2) parent population is cultivated: select crude protein content to be at least 38% manufactured feed and throw something and feed, within antenatal 3 months, need strengthen cultivating: select good, the water velocity of water quality between 0.1 ~ 0.2m/s, water temperature cultivates in the cold water environment of 0 ~ 20 DEG C, antenatal 15 ~ 20 days injection LRH-A
20.2 μ g accelerates the ripening, and every day gives stimulation by running water;
(3) artificial induced spawning: for the raun that maturity is good, water temperature can be hastened parturition 7 ~ 12 DEG C time, and oxytocic drug and consumption are: LRH-A
2every kg body weight is 3 ~ 6 μ g; The every kg body weight of HCG is 1000 ~ 1500IU, point 2 injections, and first time injects as the remaining all dosage of the 1/8 ~ 1/6,2nd pin injection of accumulated dose, and milter all adopts disposable injection, and dosage is 1/2 of raun accumulated dose, adopts dry method of artificial fertilization;
(4) hatch: adopt net cage hatching or Lotic hatching: adopt net cage hatching, at least 5cm placed under water by net cage, Spawning water is Natural Water, dissolved oxygen >=5mg/L, hatching water temperature 10 ~ 19 DEG C, pH value is 7.2-8.0, adopts Lotic hatching, bad ovum should be selected in time in hatching, ovum of not inseminating, dead ovum, incubator box will avoid illumination;
(5) fry rearing: maintain aforementioned hatching process until prelarva yolk sac absorbs complete, yolk sac should throw something and feed microzooplankton till flat trip after disappearing, fry rearing water temperature 15 ~ 20 DEG C.
2. Yellow River fish artificial fecundation method as claimed in claim 1, it is characterized in that the specific practice of employing dry method of artificial fertilization is: after the raun dry towel of maturation is dried body surface water, squeeze ovum engagement, clamp-on seminal fluid simultaneously, 2 ~ 3min is stirred with feather, sperm is fully contacted with ovum, now add the clear water identical with Spawning water at least 500mL again, activate sperm and ovum, continue to stir at least after 1min, add at least unnecessary seminal fluid of 500mL clear water rinsed clean and foreign material, pour incubator box hatching into, when carrying out artificial insemination, should note avoiding sunlight direct irradiation sperm and ovum.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103875572B (en) * | 2014-04-10 | 2015-08-05 | 中国科学院昆明动物研究所 | It is a kind of that soft thorn is naked splits the artificial propagation of buttocks fish and hatch fish roe method |
| CN106818550A (en) * | 2016-12-28 | 2017-06-13 | 贵州锦润水产品有限责任公司 | A kind of naked cultural method for splitting buttocks fish of soft thorn |
| CN106879510B (en) * | 2017-04-12 | 2019-04-19 | 西藏自治区农牧科学院水产科学研究所 | The naked induced spawning method for splitting buttocks fish in Lhasa |
| CN109496925B (en) * | 2018-12-24 | 2021-07-09 | 华电云南发电有限公司 | Fry breeding method of schizopygmy marigold |
| CN109588585A (en) * | 2019-01-29 | 2019-04-09 | 华电云南发电有限公司 | It is a kind of to prevent and treat the soft naked feed for fingerling for splitting buttocks fish parasite disease of thorn |
| CN111955386A (en) * | 2020-07-26 | 2020-11-20 | 长江水利委员会长江科学院 | Artificial breeding method of Qinghai-Tibet plateau small-head nojirimus fish |
| CN115119780A (en) * | 2022-08-02 | 2022-09-30 | 云南大学 | Method for field artificial propagation of Diandi phylloxera |
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| KR101281396B1 (en) * | 2012-11-06 | 2013-07-02 | 충청남도 | Spawning induction by change of environment for takifugu |
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Patent Citations (3)
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| RU2386248C2 (en) * | 2008-06-23 | 2010-04-20 | Институт проблем экологии и эволюции им. А.С. Северцева Российской академии наук (ИПЭЭ РАН) | Method of spawn incubation in artificial nests-incubators arranged in rivers |
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