CN103875572B - It is a kind of that soft thorn is naked splits the artificial propagation of buttocks fish and hatch fish roe method - Google Patents

Abstract

What the present invention relates to is a kind of about artificial freshwater-fish breeding and hatch fish roe method, is specially that a kind of soft thorn is naked splits the artificial propagation of buttocks fish and hatch fish roe method, belongs to artificial propagation of fish and hatch fish roe technical field; A kind of soft thorn of the present invention is naked splits the artificial propagation of buttocks fish and hatch fish roe method mainly comprises: parent population is selected, hasten parturition, artificial insemination, hatch fish roe and fry raising step; Soft thorn of the present invention is naked splits the artificial propagation of buttocks fish and hatch fish roe method, and its advantage is: 1, fertilization rate is up to 89.6%, and incubation rate, up to 95%, for the stock number caused because of environmental problem is deficient, provides solution route; 2, the present invention makes fry hatching process convenient operation, increases controllability, reduces the requirement to manpower and management in hatching process.

Description

It is a kind of that soft thorn is naked splits the artificial propagation of buttocks fish and hatch fish roe method

Technical field:

What the present invention relates to is a kind of about artificial freshwater-fish breeding and hatch fish roe method, is specially that a kind of soft thorn is naked splits the artificial propagation of buttocks fish and hatch fish roe method, belongs to artificial propagation of fish and hatch fish roe technical field.

Background technology:

Soft thorn is naked to be split buttocks fish (Schizopygopsis) and is under the jurisdiction of Cypriniformes Cypriniforms, Cyprinidae Cyprinidae, Schizothoracinae Schizothoracinae, Schizopygopsis Schizopygopsis Steindachner, is commonly called as native fish, smallmouth fish.Soft thorn Schizopygopsis is in cold water fishes, and poor growth, lives in the broad valley in the river, plateau of water quality clarification, with algae or aquatic insect for food more.Mainly be distributed in Jinsha jiang River of Upper Yangtze River, Yalongjiang River water system, its distribution height can reach height above sea level 3000m-4000m.The naked individual weight of buttocks fish of splitting of soft thorn is generally at 0.5kg-1kg, and when build is larger, weight can reach 5kg.In recent years, due to climatic variation, water pollution, causes the naked quantity splitting buttocks fish of soft thorn to reduce gradually, visible, and the naked technology splitting buttocks fish of the research soft thorn of artificial propagation has very large necessity.

By literature search, the report identical with the present invention is had no.

Summary of the invention:

The object of the present invention is to provide a kind of spawning rate up to 93.8%, fertilization rate is up to 89.6%, and incubation rate nakedly splits the artificial propagation of buttocks fish and hatch fish roe method up to the soft thorn of 95%, is that the stock number that causes because of environmental problem is deficient, provides solution route.

The present invention adopts the Lotic hatching device after improvement, makes fry hatching process convenient operation, increases controllability, reduces the requirement to manpower and management in hatching process.

The present invention is a kind of, and soft thorn is naked splits the artificial propagation of buttocks fish and hatch fish roe method, it is characterized in that the concrete steps of the method are as follows:

A. parent population is selected: gather field parent population, and through pond, examination is supported, and after acclimatization culture, chooses and is not hurt, and the parent population of physical health is individual; Parent population is supported temporarily at long 200cm, wide 100cm, in the safety glass cylinder of high 100cm, in cylinder, culvert system is set, make in whole cylinder, to keep water quality clean; In cylinder, water quality transparency is 100cm or 110cm, dissolved oxygen 5.4mg/L or 6.8mg/L, pH7.0 or pH7.5, osmotic pressure 3mOsm/kg H ₂o or 5mOsm/kg H ₂o, temperature is 14 DEG C or 15 DEG C or 16; DEG C

B. hasten parturition: split buttocks fish carry out artificial induced spawning to sexually matured soft thorn is naked, ocyodinic is by luteotropin releasing hormone d-ala analog (LHRH-A ₂)+maleic acid DOM (DOM)+carp brain hypophysis (PG) composition, injection measurement is by per kilogram of body weight 1 μ g luteotropin releasing hormone d-ala analog (LHRH-A ₂)+1mg maleic acid DOM (DOM)+5 μ g carp brain hypophysis (PG), the injection measurement of milter subtracts 30%; The osmotic pressure of injection is adjusted to 285mOsm/kg H ₂o or 310mOsm/kg H ₂o; During injection ocyodinic, parent population is taken out by cylinder by one people, both hands gently hold fish body back of head respectively and caudal peduncle is anterior, fish body belly down, ocyodinic is injected by fish body muscle of back by another people, syringe needle becomes 45 degree or 55 degree with fish body, and syringe needle selects No. 7, syringe Capacity Selection 10ml; The parent population of having injected ocyodinic puts into the glass jar of equivalent specifications, has feeding/discharging device in glass jar, and temperature is 16, DEG C dissolved oxygen 5.4mg/L or 6.8mg/L, pH7.0 or pH7.5, osmotic pressure 3mOsm/kg H ₂o or 5mOsm/kg H ₂o, carried out artificial aiding-birth after 20 hours;

C. artificial dry method fertilization: refer to light pressure fish body belly with two, extrude by after fish body forward direction; The fish-egg extruded and seminal fluid, be contained in clean bowl, stir gently with the writing brush of softness, leaves standstill 30 seconds; Use osmotic pressure is 285mOsm/kg H ₂o or 310mOsm/kg H ₂the physiological saline of O rinses the mixed fish-egg seminal fluid mixture with seminal fluid gently, and the writing brush of flushing limit, limit softness stirs fish-egg seminal fluid mixture clockwise gently and removes unnecessary seminal fluid, is then laid in by fish-egg in clean hatching cylinder;

D. hatch fish roe: hatching cylinder is each 100cm of length and width, the glass jar of high 20cm, casing wall one side bottom has a diameter to be the aperture of 2cm, and have a straight tube in hole, straight tube overall diameter is equal with hole diameter; Valve is equipped with in the side of straight tube, is convenient to draining, and opposite side cover has close screen pack processed; Each glass jar draws by a flexible pipe and enters cylinder from the water at same water source, all arranges valve, so that regulating water flow in the middle part of each flexible pipe; The water flowed out in cylinder imports drainage channel, finally flows out hatching house; Be evenly distributed in hatching cylinder to make ovum, the plastic plate of one piece of thick 2mm is placed in hatching cylinder, respectively there is the pin of a 10mm corner of plastic plate one side, on plastic plate, 5mm × 5mm distributes as net shape the aperture of 2mm, during hatching, fertilized egg is stuck on aperture, has certain gap between ovum and ovum, prevent demersal egg growing because of the in heaps and mould of current, be convenient in hatching process, choose dead ovum; Every cylinder puts 3500, the fish-egg or 4750 or 6000 that ovum footpath is 3.22mm or 3.60mm, treats that fish-egg enters cylinder, adds clear water, and the water yield is ± 5cm on ovum; In hatching cylinder, water temperature controls at 10 DEG C or 13 DEG C or 16, DEG C pH7.0 or pH7.5, dissolved oxygen 5.4mg/L or 6.8mg/L, osmotic pressure 3mOsm/kg H ₂o or 5mOsm/kgH ₂o; Hatching house's window is drawn the curtain together, and avoids fish-egg embryo to produce lopsided fry by high light radiation; An air interchanger respectively installed by four sides, hatching house wall, keeps the air circulation of hatching house; Hatch fish roe is after 253 hours, and fry starts to hatch, and hatch after 361 hours, fry hatching terminates;

E. fry is raised: after the feed of fry opening, to throw something and feed every day pulpous state bait, bait is boil rear yolk, now mill soya bean soya-bean milk and the mash feed mixture containing 45% crude protein, yolk, soya bean and mash feed mass percent are 1:1:1, when preparing, per kilogram bait adds vitamin C 10 grams, multivitamin and minerals tablet 5 grams, and mixed feed and water quality percentage are 1:4; Throw something and feed metering for every thousand tail fish 10ml; Fry opening ± 15 day, sooner or later throw something and feed every day hay bacillus and saccharomycete, feeding volume is 4,000,000/m ²or 5,000,000/m ²or 6,000,000/m ², every morning 10:00 presses 20 grams/m with nitrifying bacteria simultaneously ²or 30 grams/m ²regulating water quality; Fry opening 30-50 days, throw something and feed wheel animalcule and 111 carp fingerling fish meal ground feeds, wheel animalcule worm feeding volume is 1,000,000/m ², the calculating of fish meal feeding volume 1% or 2% feed coefficient is is by mass percentage thrown something and fed; Opening is after 50 days, throw something and feed fish meal by mass percentage 2% or 5% feed coefficient calculate and throw something and feed.

The invention has the advantages that:

1, fertilization rate is up to 89.6%, and incubation rate, up to 95%, for the stock number caused because of environmental problem is deficient, provides solution route.

2, the present invention makes fry hatching process convenient operation, increases controllability, reduces the requirement to manpower and management in hatching process.

Embodiment:

During 21:00 on the 31st May in 2013, according to following method, be one group by male and female parent population with ratio 1:3, select 8 groups of parent populations injection ocyodinics.Carry out artificial insemination to successful 30 tail parent populations of hastening parturition during 17:00 on the 1st June in 2013, obtain 32700 fertilized eggs altogether, fertilization rate reached 89.6%, hatching terminates, and obtain fry 31070 tail altogether, incubation rate reaches 95%.Detailed process is as follows:

A, parent population select: selected parent population is all in 2006, successively with acquisition modes such as cages in the collection of the ground such as Jinsha jiang River and Yalongjiang River, weight range is at 0.62-0.98kg; From the parent population of field acquisition, successively through pond, examination is supported, acclimatization culture; During 16:00 on the 28th May in 2013, choose and be not hurt, male and female parent population 50 tail of physical health, supports at long 200cm, wide 100cm temporarily, in the safety glass cylinder of high 100cm, arranges culvert system in cylinder, makes to keep water quality clean in whole cylinder; In cylinder, water quality transparency is 100cm or 110cm, dissolved oxygen 5.4mg/L or 6.8mg/L, pH7.0 or pH7.5, osmotic pressure 3mOsm/kg H ₂o or 5mOsm/kg H ₂o, temperature is 14 DEG C or 15 DEG C or 16; DEG C

B, to hasten parturition: during 21:00 on the 31st May in 2013, be one group by female-male proportion 1:3, in safety glass cylinder, select 32 tail parent populations 50 tail parent populations, give each tail parent population injection ocyodinic respectively; Ocyodinic is by luteotropin releasing hormone d-ala analog A ₂(LHRH-A ₂)+maleic acid DOM (DOM)+carp brain hypophysis composition (PG), injection measurement is by per kilogram of body weight 1 μ g luteotropin releasing hormone d-ala analog (LHRH-A ₂)+1mg maleic acid DOM (DOM)+5 μ g carp brain hypophysis (PG), the injection measurement of milter subtracts 30%; According to the soft thorn naked osmotic pressure splitting buttocks fish body fluid, the osmotic pressure of physiological saline is adjusted to 285mOsm/kg H ₂o or 310mOsm/kg H ₂o; During injection ocyodinic, parent population is taken out by cylinder by one people, both hands gently hold fish body back of head respectively and caudal peduncle is anterior, fish body belly down, ocyodinic is injected by fish body muscle of back by another people, syringe needle becomes 45 degree or 55 degree with fish body, and syringe needle selects No. 7, syringe Capacity Selection 10ml; The parent population of having injected ocyodinic puts into the glass jar of equivalent specifications, has feeding/discharging device in glass jar, and temperature is 16, DEG C dissolved oxygen 5.4mg/L or 6.8mg/L, pH7.0 or pH7.5, and salinity 1.5 ‰ or 3 ‰, carried out artificial aiding-birth after 20 hours;

C, artificial dry method fertilization: during 17:00 on the 1st June in 2013, the parent population after hastening parturition is mentioned gently, wipe fish surface excessive moisture with gauze; Refer to light pressure fish body belly with two, extrude by after fish body forward direction; The fish-egg extruded and seminal fluid, be contained in clean bowl, stir gently with the writing brush of softness, leaves standstill 30 seconds; According to soft thorn, naked to split buttocks milt be 285mOsm/kg H at osmotic pressure ₂o or 310mOsm/kg H ₂when O, sperm viability is best result of study, and use osmotic pressure is 285mOsm/kg H ₂o or 310mOsm/kg H ₂the physiological saline of O rinses the mixed fish-egg seminal fluid mixture with seminal fluid gently, and limit is rinsed, and the writing brush of limit softness stirs fish-egg seminal fluid mixture clockwise gently, is then laid in by fish-egg in clean hatching cylinder to remove unnecessary seminal fluid; 8 groups of parent populations, have 30 tails and hasten parturition successfully, obtain 36500, fish-egg altogether;

D, hatch fish roe: hatching cylinder is each 100cm of length and width, the glass jar of high 20cm, casing wall one side bottom has a diameter to be the aperture of 2cm, and have a straight tube in hole, straight tube overall diameter is equal with hole diameter; Valve is equipped with in the side of straight tube, is convenient to draining, and opposite side cover has close screen pack processed; Each glass jar draws by a flexible pipe and enters cylinder from the water at same water source, all arranges valve, so that regulating water flow in the middle part of each flexible pipe; The water flowed out in cylinder imports drainage channel, and finally flow out hatching house, above device forms a flowing water hatching system; Be evenly distributed in hatching cylinder to make ovum, the plastic plate of one piece of thick 2mm is placed in hatching cylinder, respectively there is the pin of a 10mm corner of plastic plate one side, on plastic plate, 5mm × 5mm distributes as net shape the aperture of 2mm, during hatching, fertilized egg is stuck on aperture, has certain gap between ovum and ovum, prevent demersal egg growing because of the in heaps and mould of current, be convenient in hatching process, choose dead ovum; Every cylinder puts 3500, the fish-egg or 4750 or 6000 that ovum footpath is 3.22mm or 3.60mm, treats that fish-egg enters cylinder, adds clear water, and the water yield is ± 5cm on ovum; In cylinder, water temperature controls 10 13 DEG C or 16, DEG C pH7.0 or pH7.5, dissolved oxygen 5.4mg/L or 6.8mg/L, make oxygen amount in hatching process sufficient; Osmotic pressure 3mOsm/kg H ₂o or 5mOsm/kg H ₂o, such hypotonic solution state, is more conducive to embryo's absorption to water in hatching; Hatching house's window is drawn the curtain together, and avoids, because high light enters hatching house, making fish-egg embryo raying and producing lopsided fry; Whole hatching house is provided with air interchanger, and four sides wall respectively installs one, and the temperature being not only conducive to hatching house regulates, and is more conducive to the air circulation of hatching house; Through observing, have 32700 fish-egg fertilizations; Hatch after 253 hours, fry starts to hatch, and hatch after 361 hours, fry hatching terminates, and hatches fry 31070 tail altogether;

E, fry are raised: after the feed of fry opening, to throw something and feed every day pulpous state bait, bait is boil rear yolk, now mill soya bean soya-bean milk and the mash feed mixture containing 45% crude protein, yolk, soya bean and mash feed mass percent are 1:1:1, when preparing, per kilogram bait adds vitamin C 10 grams, multivitamin and minerals tablet 5 grams, mixed feed and water quality percentage are 1:4, and metering of specifically throwing something and feeding is for every thousand tail fish 10ml; Fry opening ± 15 day, sooner or later throw something and feed every day hay bacillus and saccharomycete, feeding volume is 4,000,000/m ²or 5,000,000/m ²or 6,000,000/m ², every morning 10:00 presses 20 grams/m with nitrifying bacteria simultaneously ²or 30 grams/m ²regulating water quality; Fry opening 30 or 50 days, mixing is thrown something and fed wheel animalcule and 111 carp fingerling fish meals (crushed material), and wheel animalcule worm feeding volume is 1,000,000/m2, and the calculating of fish meal feeding volume 1% or 2% feed coefficient is is by mass percentage thrown something and fed; Opening is after 50 days, throw something and feed fish meal by mass percentage 2% or 5% feed coefficient calculate and throw something and feed.

Claims (1)

1. soft thorn is naked splits the artificial propagation of buttocks fish and a hatch fish roe method, it is characterized in that the concrete steps of the method are as follows:

A. parent population is selected: gather field parent population, and through pond, examination is supported, and after acclimatization culture, chooses and is not hurt, and the parent population of physical health is individual; Parent population is supported temporarily at long 200cm, wide 100cm, in the safety glass cylinder of high 100cm, in cylinder, culvert system is set, make in whole cylinder, to keep water quality clean; In cylinder, water quality transparency is 100cm or 110cm, dissolved oxygen 5.4mg/L or 6.8mg/L, pH7.0 or pH7.5, osmotic pressure 3mOsm/kg H ₂o or 5mOsm/kg H ₂o, temperature is 14 DEG C or 15 DEG C or 16 DEG C;

B. hasten parturition: split buttocks fish carry out artificial induced spawning to sexually matured soft thorn is naked, ocyodinic is by luteotropin releasing hormone d-ala analog LHRH-A ₂+ maleic acid DOM DOM+ carp brain hypophysis PG forms, and injected dose is by per kilogram of body weight 1 μ g luteotropin releasing hormone d-ala analog LHRH-A ₂+ 1mg maleic acid DOM DOM+5 μ g carp brain hypophysis PG, the injected dose of milter subtracts 30%; The osmotic pressure of ocyodinic is adjusted to 285mOsm/kg H ₂o or 310mOsm/kg H ₂o; During injection ocyodinic, parent population is taken out by cylinder by one people, both hands gently hold fish body back of head respectively and caudal peduncle is anterior, fish body belly down, ocyodinic is injected by fish body muscle of back by another people, syringe needle becomes 45 degree or 55 degree with fish body, and syringe needle selects No. 7, syringe Capacity Selection 10ml; The parent population of having injected ocyodinic puts into the glass jar of equivalent specifications, has feeding/discharging device in glass jar, and temperature is 16 DEG C, dissolved oxygen 5.4mg/L or 6.8mg/L, pH7.0 or pH7.5, osmotic pressure 3mOsm/kg H ₂o or 5mOsm/kg H ₂o, carried out artificial aiding-birth after 20 hours;

C. artificial dry method insemination: refer to light pressure fish body belly with two, extrude by after fish body forward direction; The fish-egg extruded and seminal fluid, be contained in clean bowl, stir gently with the writing brush of softness, leaves standstill 30 seconds; Use osmotic pressure is 285mOsm/kg H ₂o or 310mOsm/kg H ₂the physiological saline of O rinses the mixed fish-egg seminal fluid mixture with seminal fluid gently, and the writing brush of flushing limit, limit softness stirs fish-egg seminal fluid mixture clockwise gently and removes unnecessary seminal fluid, is then laid in by fish-egg in clean hatching cylinder;

D. hatch fish roe: hatching cylinder is each 100cm of length and width, the glass jar of high 20cm, place the plastic plate of one piece of thick 2mm in hatching cylinder, respectively there is the pin of a 10mm corner of plastic plate one side, and plastic plate is that 5mm × 5mm is latticed, be distributed with the aperture of 2mm, during hatching, fertilized egg is stuck on aperture, has certain gap between ovum and ovum, prevent demersal egg growing because of the in heaps and mould of current, be convenient in hatching process, choose dead ovum; Every cylinder puts 3500, the fish-egg or 4750 or 6000 that ovum footpath is 3.22mm or 3.60mm, treats that fish-egg enters cylinder, adds clear water, and the water yield is ± 5cm on ovum; In hatching cylinder, water temperature controls at 10 DEG C or 13 DEG C or 16 DEG C, pH7.0 or pH7.5, dissolved oxygen 5.4mg/L or 6.8mg/L, osmotic pressure 3mOsm/kg H ₂o or 5mOsm/kg H ₂o; Hatching house's window is drawn the curtain together, and avoids fish-egg embryo to produce lopsided fry by high light radiation; An air interchanger respectively installed by four sides, hatching house wall, keeps the air circulation of hatching house; Hatch fish roe is after 253 hours, and fry starts to hatch, and hatch after 361 hours, fry hatching terminates;

E. fry is raised: after the feed of fry opening, to throw something and feed every day pulpous state bait, bait is boil rear yolk, now mill soya bean soya-bean milk and the mash feed mixture containing 45% crude protein, yolk, soya bean soya-bean milk and mash feed mass percent are 1:1:1, when preparing, per kilogram bait adds vitamin C 10 grams, multivitamin and minerals tablet 5 grams, and mixed feed and water quality percentage are 1:4; Dosage of throwing something and feeding is every thousand tail fish 10ml; Fry opening ± 15 day, sooner or later throw something and feed every day hay bacillus and saccharomycete, feeding volume is 4,000,000/m ²or 5,000,000/m ²or 6,000,000/m ², every morning 10:00 presses 20 grams/m with nitrifying bacteria simultaneously ²or 30 grams/m ²regulating water quality; Fry opening 30-50 days, throw something and feed wheel animalcule and 111 carp fingerling fish meal ground feeds, wheel animalcule feeding volume is 1,000,000/m ², the calculating of 111 carp fingerling fish meal ground feed feeding volumes, 1% or 2% feed coefficient is is by mass percentage thrown something and fed; Opening is after 50 days, throw something and feed 111 carp fingerling fish meal ground feeds by mass percentage 2% or 5% feed coefficient calculate and throw something and feed.