CN107295987B - Method for artificially induced spawning of egg-shaped pompano - Google Patents
Method for artificially induced spawning of egg-shaped pompano Download PDFInfo
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- CN107295987B CN107295987B CN201710561053.4A CN201710561053A CN107295987B CN 107295987 B CN107295987 B CN 107295987B CN 201710561053 A CN201710561053 A CN 201710561053A CN 107295987 B CN107295987 B CN 107295987B
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- 238000000034 method Methods 0.000 title claims abstract description 18
- 241000276694 Carangidae Species 0.000 title description 8
- 241000251468 Actinopterygii Species 0.000 claims abstract description 103
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 74
- 241000719209 Trachinotus ovatus Species 0.000 claims abstract description 68
- 239000013535 sea water Substances 0.000 claims abstract description 19
- 230000008859 change Effects 0.000 claims abstract description 11
- 238000002347 injection Methods 0.000 claims abstract description 10
- 239000007924 injection Substances 0.000 claims abstract description 10
- 210000003205 muscle Anatomy 0.000 claims abstract description 4
- 210000000689 upper leg Anatomy 0.000 claims abstract description 4
- 230000006698 induction Effects 0.000 claims description 7
- 230000009182 swimming Effects 0.000 claims description 7
- 102000011022 Chorionic Gonadotropin Human genes 0.000 claims description 4
- 108010062540 Chorionic Gonadotropin Proteins 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 239000000579 Gonadotropin-Releasing Hormone Substances 0.000 claims description 4
- 101000857870 Squalus acanthias Gonadoliberin Proteins 0.000 claims description 4
- 229940015047 chorionic gonadotropin Drugs 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- XLXSAKCOAKORKW-AQJXLSMYSA-N gonadorelin Chemical compound C([C@@H](C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1[C@@H](CCC1)C(=O)NCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@H]1NC(=O)CC1)C1=CC=C(O)C=C1 XLXSAKCOAKORKW-AQJXLSMYSA-N 0.000 claims description 4
- 229940035638 gonadotropin-releasing hormone Drugs 0.000 claims description 4
- 229920000742 Cotton Polymers 0.000 claims description 2
- 206010002091 Anaesthesia Diseases 0.000 description 14
- 230000037005 anaesthesia Effects 0.000 description 14
- 230000003444 anaesthetic effect Effects 0.000 description 6
- 206010021143 Hypoxia Diseases 0.000 description 4
- 238000001949 anaesthesia Methods 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 235000013601 eggs Nutrition 0.000 description 3
- 230000001568 sexual effect Effects 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 230000032696 parturition Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000009278 visceral effect Effects 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 229940121710 HMGCoA reductase inhibitor Drugs 0.000 description 1
- 244000062730 Melissa officinalis Species 0.000 description 1
- 235000010654 Melissa officinalis Nutrition 0.000 description 1
- 241000604373 Ovatus Species 0.000 description 1
- 241000276618 Perciformes Species 0.000 description 1
- 241000719226 Trachinotus Species 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000002431 foraging effect Effects 0.000 description 1
- 239000003163 gonadal steroid hormone Substances 0.000 description 1
- 239000000865 liniment Substances 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000008159 sesame oil Substances 0.000 description 1
- 235000011803 sesame oil Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a method for artificially induced spawning of Trachinotus ovatus, which comprises the following steps: s101, placing a water bag in a net cage of a spawning area, and adding seawater into the water bag; s102, fishing out the trachinotus ovatus parent fish to be induced to spawn, putting the trachinotus ovatus parent fish into the water bag, struggling the trachinotus ovatus parent fish in the water bag, and observing the change characteristics of the trachinotus ovatus parent fish in the water bag; s103, according to the change characteristics of the parent fishes of the trachinotus ovatus in the water bag in the S102, one operator drags the parent fishes of the trachinotus ovatus with a draging skip, the other operator clamps the tail handles of the parent fishes by using the index finger and the middle finger of the left hand and leans against the tail fins, and the parent fishes are inverted in the draging skip and cling to the right thighs of the operators; s104, the fish is held by the left hand, the injector is held by the right hand to be aligned with the trunk of the parent fish, and injection is carried out at the muscle position of the caudal peduncle. The method can effectively reduce the stress reaction of the trachinotus ovatus parent fish during artificial induced spawning.
Description
Technical Field
The invention relates to the field of trachinotus ovatus propagation, in particular to a method for reducing stress reaction of trachinotus ovatus parent fish during artificial induced spawning.
Background
Trachinotus ovatus (Prachinotus ovatus), commonly known as golden pompano, yellow wax pompano, belongs to the order Perciformes, the family of trachinotus. The trachinotus ovatus fish is tender, delicious in taste and high in nutritive value, is a famous and precious edible fish and is deeply popular in the market. Meanwhile, the trachinotus ovatus grows fast, has strong disease resistance, high yield and good benefit, and is a very popular breeding variety for farmers. Oval pompano has a migration foraging habit, oxygen must be absorbed through swimming, and the swinging and breaking-out force of the pompano is higher than that of general fishes when the fishes leave water as one of fishes with the fastest swimming speed.
At present, a farmer carries out artificial induced spawning on trachinotus ovatus parent fish to reduce stress reaction and avoid injury and adding anesthetic, the induced spawning method is that a water bag is arranged, seawater is filled in the water bag, the trachinotus ovatus parent fish of about 20 is fished, anesthetic sesame oil and water statin are added to the water bag for anesthesia, then the water bag is taken out and placed on an operation table, and three persons carry out induced spawning on the trachinotus ovatus parent fish, as the trachinotus ovatus parent fish are fished into the water bag in batches, the sizes of the fish bodies are different, the fish bodies are easy to bounce due to small dosage, the fish bodies are easy to stab for multiple times when sex hormone is injected, three persons are required to observe the anesthesia condition and fish to the operation table, and one person presses the fish head and the tail to carry out injection; the dosage is more, although the artificial induced spawning is easy to carry out, the trachinotus ovatus cannot move in a short time, and the trachinotus ovatus is dead due to oxygen deficiency.
The artificial cultivation of the trachinotus ovatus needs 4 to 5 years for sexual maturity, the cultivation time and the nutrition cost are obviously higher than those of the common finished product fish cultivation, and the conventional artificial spawning induction method is easy to cause death and injury of the parent fish, so that the method for effectively reducing the stress reaction of the trachinotus ovatus parent fish during the artificial spawning induction without using an anesthetic is particularly important.
Disclosure of Invention
The invention aims to overcome the problems in the prior art and provide the artificial induced spawning method for the trachinotus ovatus parent fish without using anesthetic.
In order to achieve the technical purpose and achieve the technical effect, the invention is realized by the following technical scheme:
an artificial spawning induction method for trachinotus ovatus comprises the following steps:
s101, placing a water bag in a net cage of a spawning area, and adding seawater filtered by a cotton bag into the water bag;
s102, fishing out the trachinotus ovatus parent fish to be induced to spawn, putting the trachinotus ovatus parent fish into the water bag, struggling the trachinotus ovatus parent fish in the water bag, and observing the change characteristics of the trachinotus ovatus parent fish in the water bag;
s103, according to the change characteristics of the parent fishes of the trachinotus ovatus in the water bag in the S102, when the walking is lack of strength, one operator drags the parent fishes of the trachinotus ovatus by using a draging skip, the other operator clamps the tail handle of the parent fishes by using the index finger and the middle finger of the left hand to be close to the tail fin, and the parent fishes are inverted in the draging skip and are tightly attached to the right thigh of the operator;
s104, the fish is held by the left hand, the injector is held by the right hand to be aligned with the trunk of the parent fish, and injection is carried out at the muscle position of the caudal peduncle.
Preferably, the water bag in S101 is a cuboid, and the volume of the water bag is 5-7m3The water bag is made of PVC canvas, and the smooth surface of the water bag is in contact with seawater.
Preferably, the seawater added to the water bag does not over the dorsal fins of the parent fish.
Preferably, the body weight of the trachinotus ovatus parent fish in the S102 is 4-6 kg, and 20-30 fishes are fished into the water bag each time.
Preferably, the change characteristics of the trachinotus ovatus parent fish in the water bag in S102 comprise: slowing down the swimming, opening and closing the mouth and settling the parent fish.
Preferably, the dose injected to the parent fish in S104 is: 1200 units of chorionic gonadotropin per kilogram, 1ml of 50% glucose per kilogram and 6 μ g gonadotropin-releasing hormone a4 per kilogram.
The invention has the beneficial effects that:
1. the existing common water bag is used for narcotizing parent fishes of trachinotus ovatus, because batch anesthesia is needed, the anesthesia amount and the anesthesia time are difficult to control, the parent fishes are put into a spawning net cage after induced spawning and can not move due to the anesthesia relationship, so that the anoxical death is caused; the invention cancels anesthesia, consumes a large amount of physical energy of the parent fishes of the trachinotus ovatus in a short time by utilizing the water bag, and compared with the anesthesia, the invention only adds a small amount of water into the water bag, aiming at leading the trachinotus ovatus to have a low oxygen state, obviously reducing shaking when the parent fishes are held by hands, being beneficial to the spawning work, and being capable of swimming without oxygen deficiency after being placed back in water.
2. Compared with a rough canvas, the water bag made of the PVC canvas smooth surface can effectively reduce scratches caused by the friction of the parent fishes of the trachinotus ovatus with the canvas.
3. The invention can hasten parturition without an operation table or an operation basin, and the number of people is reduced from three to two; and the original flat injection is changed into inverted injection, so that the visceral area is not easy to be punctured by mistake when parent fishes of pompano shake; the operation process is simple and easy to learn by hands in a short time.
The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical means of the present invention more clearly understood and to be implemented in accordance with the content of the description, the specific embodiments of the present invention are given in detail by the following examples.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples
The method for reducing the stress reaction of parent fishes of trachinotus ovatus during artificial spawning induction comprises the following steps:
s101, placing a water bag in a net cage of a spawning area, and adding seawater into the water bag; the water bag is a cuboid, and the volume of the water bag is 5-7m3Hereinafter, the number is 6m3For example, the water bag is made of PVC canvas, the smooth surface of the water bag is in contact with seawater, and the water bag made of PVC canvas can effectively reduce scratches caused by friction between parent fishes of the trachinotus ovatus and the canvas compared with the coarse canvas.
Adding seawater into water bag to a depth of about 1m3The seawater is used for controlling the parent fish to submerge dorsal fins, the seawater is not too much or too little, if the seawater completely submerges the parent fish, the parent fish cannot lack oxygen to release physical energy, and if the seawater is too little, the parent fish can die due to lack of water.
S102, fishing out the trachinotus ovatus parent fish to be induced to spawn, putting the trachinotus ovatus parent fish into the water bag, struggling the trachinotus ovatus parent fish in the water bag, observing the change characteristics of the trachinotus ovatus parent fish in the water bag, mainly observing the change characteristics of swimming, sinking and floating, oxygen deficiency and water quality, and representing that a large amount of energy is released when the parent fish has the phenomena of slow swimming, mouth opening and closing, sedimentation and the like.
In S102, the weight of the trachinotus ovatus parent fish is 4-6 kg, and 20-30 fishes are fished each time and put into the water bag.
S103, according to the change characteristics of the parent fishes of the trachinotus ovatus in the water bag in the S102, one operator drags the parent fishes of the trachinotus ovatus with a draging skip, the other operator clamps the tail handles of the parent fishes by using the index finger and the middle finger of the left hand and leans against the tail fins, and the parent fishes are inverted in the draging skip and cling to the right thighs of the operators;
s104, the fish is held by the left hand, the injector is held by the right hand to be aligned with the trunk of the parent fish, and injection is carried out at the muscle position of the caudal peduncle.
The dose injected to the parent fish in S104 is as follows: 1200 units of chorionic gonadotropin per kilogram, 1ml of 50% glucose per kilogram and 6 μ g gonadotropin-releasing hormone a4 per kilogram.
Comparative example
S201, placing a water bag in a net cage of a spawning area, and adding seawater into the water bag; the water bag is a cuboid and has a volume of 6m3The water bag is made of PVC canvas, the smooth surface of the water bag is in contact with seawater, and compared with rough surface canvas, the water bag made of the smooth surface of PVC canvas can effectively reduce scratches caused by friction of parent fishes of the trachinotus ovatus;
s202, adding seawater into a water bag, and then adding anesthetic, namely the balm, the flutemastine to obtain a water source containing anesthetic;
s203, fishing out the trachinotus ovatus parent fish to be induced to spawn, putting the trachinotus ovatus parent fish into the water bag, wherein the weight of the trachinotus ovatus parent fish is 4-6 kg, and fishing 20-30 fishes each time and putting the trachinotus ovatus parent fish into the water bag;
s204, fishing out the trachinotus ovatus parent fish to be induced to spawn, placing the trachinotus ovatus parent fish on an operation table, and carrying out induced spawning injection on the trachinotus ovatus parent fish by three persons; the injected dose was: 1200 units of chorionic gonadotropin per kilogram, 1ml of 50% glucose per kilogram and 6 μ g gonadotropin-releasing hormone a4 per kilogram.
In the embodiment, the success rate of the concentrated induced spawning of 25 parent fishes is 100%, the spawning rate of 90% of sexual maturity is 100%, the spawned eggs are good in quality, individuals are full, the rate of dead eggs sinking is less than 5%, the hatching rate is 70%, and the rate of emergence is 90%.
In the comparative example, the success rate of the concentrated induced spawning of 25 parent fishes is 80%, the spawning rate of 90% of sexual maturity is 80%, the rate of dead eggs sinking reaches 15%, the hatching rate is 50% and the rate of emergence is 75%.
The water bag in the comparative example is used for anaesthetizing parent fishes of trachinotus ovatus, and because batch anaesthesia is needed, the anaesthesia amount and the anaesthesia time are difficult to control, the parent fishes are put into the spawning net cage after induced spawning and can not move due to the anaesthesia relationship to cause anoxic death; in the embodiment, anesthesia is eliminated, a large amount of physical energy of the parent fishes of the trachinotus ovatus is consumed in a short time by using the water bag, and compared with the anesthesia, the invention only adds a small amount of water into the water bag, aims to ensure that the trachinotus ovatus appears in a low oxygen state, obviously reduces shaking when a hand holds the parent fishes, is favorable for the spawning induction work, and can swim in the water without oxygen deficiency.
In the embodiment, the hasten parturition can be carried out without an operation table or an operation basin, and the number of people is reduced from three to two; and the visceral area is not easy to be punctured by mistake when parent fishes of pompano shake from the horizontal injection of the comparative example to the inverted injection; the operation process is simple and easy to learn by hands in a short time.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (5)
1. An artificial spawning induction method for trachinotus ovatus is characterized by comprising the following steps: s101, placing a water bag in a net cage of a spawning area, adding seawater filtered by a cotton bag into the water bag, and adding the seawater into the water bag to enable the seawater level to submerge fins of parent fishes; s102, fishing out the trachinotus ovatus parent fish to be induced to spawn, putting the trachinotus ovatus parent fish into the water bag, struggling the trachinotus ovatus parent fish in the water bag, and observing the change characteristics of the trachinotus ovatus parent fish in the water bag; s103, according to the change characteristics of the parent fishes of the trachinotus ovatus in the water bag in the S102, when the walking is lack of strength, one operator drags the parent fishes of the trachinotus ovatus by using a draging skip, the other operator clamps the tail handle of the parent fishes by using the index finger and the middle finger of the left hand to be close to the tail fin, and the parent fishes are inverted in the draging skip and are tightly attached to the right thigh of the operator; s104, the fish is held by the left hand, the injector is held by the right hand to be aligned with the trunk of the parent fish, and injection is carried out at the muscle position of the caudal peduncle.
2. The artificial spawning induction method for trachinotus ovatus as claimed in claim 1, wherein the water bag in S101 is cuboid and has a volume of 5-7m3The water bag is made of PVC canvas, and the smooth surface of the water bag is in contact with seawater.
3. The artificial spawning method for trachinotus ovatus according to claim 1, wherein the weight of the trachinotus ovatus parent fishes in S102 is 4-6 kg, and 20-30 fishes per time are fished and put into the water bag.
4. The artificial spawning method for trachinotus ovatus according to claim 1, wherein the change characteristics of trachinotus ovatus parent fishes in the water bag in S102 comprise: slowing down the swimming, opening and closing the mouth and settling the parent fish.
5. The artificial induced spawning method for trachinotus ovatus according to claim 1, wherein the dose injected to the parent fishes in the step S104 is as follows: 1200 units of chorionic gonadotropin per kilogram, 1ml of 50% glucose per kilogram and 6 μ g gonadotropin-releasing hormone a4 per kilogram.
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| CN201710561053.4A CN107295987B (en) | 2017-07-11 | 2017-07-11 | Method for artificially induced spawning of egg-shaped pompano |
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| CN111374073B (en) * | 2020-05-09 | 2022-04-08 | 陵水德林诚信水产养殖有限公司 | Artificial spawning induction method for ovate pompano |
| CN112293312A (en) * | 2020-11-24 | 2021-02-02 | 广东大麟洋海洋生物有限公司 | Cross breeding method for golden pomfret |
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| CN101642067B (en) * | 2009-08-21 | 2011-09-07 | 江阴市申港三鲜养殖有限公司 | Method for artificially hastening parturition of American reeves shads, inseminating and hatching germ cells |
| CN103222436B (en) * | 2013-05-15 | 2014-07-09 | 中国水产科学研究院黑龙江水产研究所 | Artificial propagation method of esox reichertii |
| CN103875572B (en) * | 2014-04-10 | 2015-08-05 | 中国科学院昆明动物研究所 | It is a kind of that soft thorn is naked splits the artificial propagation of buttocks fish and hatch fish roe method |
| CN106804477A (en) * | 2015-11-30 | 2017-06-09 | 宁陕县绿康农业生态科技有限公司 | A kind of artificial fecundation method of Pelteobagrus fulvidraco |
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