CN112293312A - Cross breeding method for golden pomfret - Google Patents
Cross breeding method for golden pomfret Download PDFInfo
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- CN112293312A CN112293312A CN202011327872.0A CN202011327872A CN112293312A CN 112293312 A CN112293312 A CN 112293312A CN 202011327872 A CN202011327872 A CN 202011327872A CN 112293312 A CN112293312 A CN 112293312A
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- 238000000034 method Methods 0.000 title claims abstract description 39
- 238000009402 cross-breeding Methods 0.000 title claims abstract description 30
- 241000630524 Taractes rubescens Species 0.000 title claims abstract description 27
- 241000719209 Trachinotus ovatus Species 0.000 claims abstract description 64
- 235000013601 eggs Nutrition 0.000 claims abstract description 50
- 241000719226 Trachinotus Species 0.000 claims abstract description 36
- 230000004720 fertilization Effects 0.000 claims abstract description 22
- 230000012447 hatching Effects 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 79
- 239000013535 sea water Substances 0.000 claims description 22
- 238000011534 incubation Methods 0.000 claims description 21
- 108700012941 GNRH1 Proteins 0.000 claims description 20
- 239000000579 Gonadotropin-Releasing Hormone Substances 0.000 claims description 20
- 210000000582 semen Anatomy 0.000 claims description 19
- 239000003795 chemical substances by application Substances 0.000 claims description 16
- 239000008062 guanidine hydrochloride buffer Substances 0.000 claims description 15
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- 239000005556 hormone Substances 0.000 claims description 10
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- 239000000203 mixture Substances 0.000 claims description 8
- 210000004681 ovum Anatomy 0.000 claims description 8
- 230000002196 ecbolic effect Effects 0.000 claims description 7
- 210000002149 gonad Anatomy 0.000 claims description 7
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- XNOPRXBHLZRZKH-UHFFFAOYSA-N Oxytocin Natural products N1C(=O)C(N)CSSCC(C(=O)N2C(CCC2)C(=O)NC(CC(C)C)C(=O)NCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(CCC(N)=O)NC(=O)C(C(C)CC)NC(=O)C1CC1=CC=C(O)C=C1 XNOPRXBHLZRZKH-UHFFFAOYSA-N 0.000 claims description 2
- 101800000989 Oxytocin Proteins 0.000 claims description 2
- 102100031951 Oxytocin-neurophysin 1 Human genes 0.000 claims description 2
- XNOPRXBHLZRZKH-DSZYJQQASA-N oxytocin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CSSC[C@H](N)C(=O)N1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(N)=O)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 XNOPRXBHLZRZKH-DSZYJQQASA-N 0.000 claims description 2
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention provides a cross breeding method of golden pomfret, which comprises the following steps: the method comprises the steps of parent selection and cultivation, artificial induced spawning, artificial fertilization, fertilized egg hatching and the like, cross breeding is carried out by taking the trachinotus ovatus fry as a male parent and the trachinotus elongatus fry as a female parent, the fertilization rate and the hatching rate are improved through the control of parent cultivation environment, the artificial induced spawning and the artificial fertilization, the fertilized eggs with good quality are obtained, and the survival rate of the trachinotus ovatus is effectively improved.
Description
Technical Field
The invention relates to the field of aquaculture, in particular to a golden pomfret cross breeding method.
Background
Trachinotus ovatus (school name: Trachinotus ovatus) is a fish of the genus Trachinotus in the family of carangidae. High body and flat side; the tail handle is thin and short and the side is flat. The head is small, the height is larger than the length, and the occipital ridge is obvious. The length of the kiss is larger than the diameter of the eye. Small eye, anterior position. Trachinotus ovatus is a warm-water mid-upper layer migratory fish which frequently inhabits in estuary gulf after the spring festival every year in the juvenile stage, has strong clustering property and moves to the deep water of the outer sea during adult fish. The fish is carnivorous fish, and the larvae and the juveniles eat various plankton and benthonic animals, mainly copepod larvae; feeding daphnia, polychetes, small bivalves and teleost to the juvenile fish; young and adult fish feed on podosomes, bivalves, mollusks, crab larvae, shrimps, small fish, etc. Is distributed in the ocean areas of Indian ocean, Pacific ocean, Atlantic ocean tropical zone and temperate zone.
Trachinotus longipes (school name: Trachinotus blochi) is a fish of the genus Trachinotus of the family Captaceae. The maximum body length was 122 cm and the body weight was 36 kg. The obvious features are a tail with deep tines and elongated anterolateral fins that look like a sickle; trachinotus media can also be identified by the highly transversely compressed body, and is thin and high. The trachinotus ovatus shape appears circular in juvenile fish from a side view, but becomes longer as the fish grows. In addition to the elongated anteroposterior fins, there are 17-21 soft dorsal rays and 16-19 soft anal rays inserted directly above the elongated anteroposterior fins. The side is bright silver and the back is cyan or brown. The abdomen sometimes appeared yellow or occasionally black spots. The fins are shown as dark grey or black.
Trachinotus ovatus and trachinotus elongatus, commonly known as golden pompanus, are trachinotus ovatus in the same genus and different species, have tender meat and delicious taste, are deeply favored by consumers, but are excessively caught, change in environment and the like, so that the quality of a cultured population is seriously reduced, and the economic benefit is obviously reduced; the hybridization can transfer the genetic material of organisms from one population to another population, an important method for increasing the biological variability is adopted, different types of parents are hybridized to obtain the recombination of variant traits, and the more excellent traits appear in filial generations.
Disclosure of Invention
In view of the above, the present invention provides a cross breeding method for golden pomfret, which solves the above problems.
The technical scheme of the invention is realized as follows: a cross breeding method of golden pomfret comprises the following steps: the method comprises the following steps:
s1, parent selection and cultivation: placing the trachinotus ovatus fry and the trachinotus elongatus fry into a culture pond, dividing the culture pond into a still water area of a flowing water area by using a movable partition plate, controlling the water temperature of the flowing water area to be 13-15 ℃, controlling the flow rate to be 0.3-1.0 m/s, controlling the water temperature of the still water area to be 25-30 ℃, taking the trachinotus ovatus fry as a male parent and the trachinotus elongatus fry as a female parent, and transferring the trachinotus ovatus elongatus fry placed into the flowing water area for culture for 15-25 days to the still water area for static culture;
s2, artificial induced spawning: artificial spawning induction is carried out on males of the trachinotus ovatus and females of the trachinotus elongatus with well developed gonads, the artificial spawning induction is carried out in a running water area for culture, the flow rate is controlled to be 0.9-1.5 m/s, the spawning induction agent of the male parent of the trachinotus ovatus is 20-30 mu g/kg of luteinizing hormone releasing hormone and 2-5 mg/kg of deovone, the spawning induction agent of the female parent of the trachinotus elongatus is 12-20 mu g/kg of luteinizing hormone releasing hormone, hormone agonist RH-A2-5 mg/kg and guanethidine 3-8 mg/kg;
s3, artificial fertilization: after 48-50 h of induced spawning, taking out the semen of the male trachinotus ovatus and the eggs of the female trachinotus ovatus, putting the sperms and the eggs into a glass cup, adding 1-3 times of seawater, stirring the mixture for 60-100 s by using a glass rod to ensure that the sperms and the eggs are mixed uniformly, wherein the volume ratio of the sperms to the eggs is 30-50: 1, and standing the mixture for 10-20 min after artificial fertilization;
s4, hatching fertilized eggs: and putting the collected floating fertilized eggs into an incubation barrel for incubation, adding seawater containing guanidine hydrochloride buffer solution into the incubation barrel, sterilizing and demolding, collecting and transferring the fertilized eggs into a culture pond for culture when the hybridized larvae hatch out of the membrane, and obtaining the golden pomfret larvae of the hybridized variety.
Further, the ratio of the male parent to the female parent in the culture pond in the step S1 is 3-7: 1.
Further, in the step S1, the water temperature in the flowing water area is 14 ℃, the flow rate is 0.7m/S, and the water temperature in the still water area is 28 ℃.
Further, the oxytocic for the male parent of the trachinotus ovatus in the step S2 is 26 mu g/kg of luteinizing hormone releasing hormone and 3mg/kg of diosdone.
Further, in the step S2, the oxytocic for the female parent of the trachinotus elongatus is 14 mu g/kg of luteinizing hormone releasing hormone, RH-A3mg/kg of hormone agonist and 5mg/kg of guanethidine.
Further, the volume ratio of the semen to the ovum in the step of S3 is 40: 1.
Further, the temperature of the seawater containing the guanidine hydrochloride buffer solution in the step S4 is 20-25 ℃.
Further, the volume ratio of the guanidine hydrochloride buffer solution to the seawater in the step S4 is 0.3-0.8: 20-50.
Compared with the prior art, the invention has the beneficial effects that:
(1) according to the method, trachinotus ovatus fry is taken as a male parent, trachinotus ovatus fry is taken as a female parent, hybridization breeding is carried out, a culture pond is arranged into a flowing area and a still water area, the parents are subjected to low-temperature water flow stress firstly, then are statically cultured in the still water area, the energy is saved, the living habits of the parents are met, and the temperature of the still water area is adjusted, so that the stress capability of the parents can be improved in the culture process, the physique of the parents is enhanced, and the gonad development is promoted;
(2) through artificial induced spawning, the proportion of an oxytocic is reasonably adjusted aiming at different pompano genera, the oxytocic is respectively injected into a male parent and a female parent, and then water flow stress in a flowing water area is adopted, so that gonad development of a fish body is facilitated, spermiation and ovulation are promoted, and the quality of sperms and ova is improved;
(3) setting the proportion between semen and ovum, combining semen and ovum through artificial fertilization, improving the fertilization rate, facilitating the fertilized ovum to come off the membrane under the seawater containing guanidine hydrochloride buffer solution, improving the hatchability and improving the fry survival rate.
Detailed Description
In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.
The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.
The materials, reagents and the like used in the examples of the present invention can be obtained commercially without specific description.
Example 1
A cross breeding method of golden pomfret comprises the following steps: the method comprises the following steps:
s1, parent selection and cultivation: selecting trachinotus ovatus fry and trachinotus elongatus fry to be placed into a culture pond, dividing the culture pond into a still water area of a flowing water area by using a movable partition plate, controlling the water temperature of the flowing water area to be 13 ℃, controlling the flow rate to be 0.3m/s, controlling the water temperature of the still water area to be 25 ℃, taking the trachinotus ovatus fry as a male parent, taking the trachinotus elongatus fry as a female parent and taking the ratio of the male parent to the female parent to be 3:1, and transferring the trachinotus ovatus elongatus fry placed into the flowing water area for culture for 15 days to the still water area for static;
s2, artificial induced spawning: artificial induced spawning is carried out on males of the trachinotus ovatus and females of the trachinotus elongatus with well developed gonads, the artificial induced spawning is placed in a running water area for culture, the flow rate is controlled to be 0.9m/s, the induced spawning agents of the male parent of the trachinotus ovatus are luteinizing hormone releasing hormone 20 mu g/kg and diosdone 2mg/kg, the induced spawning agents of the female parent of the trachinotus elongatus are luteinizing hormone releasing hormone 12 mu g/kg, hormone agonists RH-A2mg/kg and guanethidine 3 mg/kg;
s3, artificial fertilization: after 48h of induced spawning, taking out the semen of the male trachinotus ovatus and the eggs of the female trachinotus ovatus macrobrachi, putting the sperms and the eggs into a glass cup, adding 1 time of seawater, stirring the mixture for 600s by using a glass rod to mix the sperms and the eggs evenly, wherein the volume ratio of the sperms to the eggs is 30:1, and standing the mixture for 10min after artificial fertilization;
s4, hatching fertilized eggs: and putting the collected floating fertilized eggs into an incubation barrel for incubation, adding seawater containing guanidine hydrochloride buffer solution into the incubation barrel, sterilizing and removing the membrane, collecting and transferring the fertilized eggs into a culture pond for culture when the hybridized larvae are incubated out of the membrane, and obtaining the golden pompano larvae of the hybridized variety.
Example 2
A cross breeding method of golden pomfret comprises the following steps: the method comprises the following steps:
s1, parent selection and cultivation: placing the trachinotus ovatus fry and the trachinotus elongatus fry into a culture pond, dividing the culture pond into a still water area of a flowing water area by using a movable partition plate, controlling the water temperature of the flowing water area to be 15 ℃, controlling the flow rate to be 1.0m/s, controlling the water temperature of the still water area to be 30 ℃, taking the trachinotus ovatus fry as a male parent, taking the trachinotus elongatus fry as a female parent and taking the ratio of the male parent to the female parent to be 7:1, and transferring the trachinotus ovatus elongatus fry placed into the flowing water area for 25 days to still water area for static culture;
s2, artificial induced spawning: artificial induced spawning is carried out on males of the trachinotus ovatus and females of the trachinotus elongatus with well developed gonads, the artificial induced spawning is placed in a running water area for culture, the flow rate is controlled to be 1.5m/s, the induced spawning agents of the male parent of the trachinotus ovatus are luteinizing hormone releasing hormone 30 mu g/kg and diospyrone 5mg/kg, the induced spawning agents of the female parent of the trachinotus elongatus are luteinizing hormone releasing hormone 20 mu g/kg, hormone agonists RH-A5mg/kg and guanethidine 8 mg/kg;
s3, artificial fertilization: taking out semen of male trachinotus ovatus and eggs of female trachinotus ovatus after 50h of induced spawning, putting into a glass cup, adding 3 times of seawater, stirring for 100s with a glass rod to mix semen and eggs uniformly, wherein the volume ratio of the semen to the eggs is 50:1, performing artificial fertilization, and standing for 20 min;
s4, hatching fertilized eggs: and putting the collected floating fertilized eggs into an incubation barrel for incubation, adding seawater containing guanidine hydrochloride buffer solution into the incubation barrel, sterilizing and removing the membrane, collecting and transferring the fertilized eggs into a culture pond for culture when the hybridized larvae are incubated out of the membrane, and obtaining the golden pompano larvae of the hybridized variety.
Example 3
A cross breeding method of golden pomfret comprises the following steps: the method comprises the following steps:
s1, parent selection and cultivation: placing the trachinotus ovatus fry and the trachinotus elongatus fry into a culture pond, dividing the culture pond into a still water area of a flowing water area by using a movable partition plate, controlling the water temperature of the flowing water area to be 14 ℃, controlling the flow rate to be 0.7m/s, controlling the water temperature of the still water area to be 28 ℃, taking the trachinotus ovatus fry as a male parent, taking the trachinotus elongatus fry as a female parent and taking the ratio of the male parent to the female parent to be 5:1, and transferring the trachinotus ovatus elongatus fry placed into the flowing water area for culture for 20 days to the still water area for static culture;
s2, artificial induced spawning: artificial induced spawning is carried out on males of the trachinotus ovatus and females of the trachinotus elongatus with well developed gonads, the artificial induced spawning is placed in a running water area for culture, the flow rate is controlled to be 1.3m/s, the induced spawning agents of the male parent of the trachinotus ovatus are luteinizing hormone releasing hormone 26 mu g/kg and diospyrone 3mg/kg, the induced spawning agents of the female parent of the trachinotus elongatus are luteinizing hormone releasing hormone 14 mu g/kg, hormone agonists RH-A3mg/kg and guanethidine 5 mg/kg;
s3, artificial fertilization: taking out semen of male trachinotus ovatus and eggs of female trachinotus ovatus after 50h of induced spawning, putting into a glass cup, adding 2 times of seawater, stirring for 80s with a glass rod to mix the semen and the eggs uniformly, wherein the volume ratio of the semen to the eggs is 40:1, and standing for 15min after artificial fertilization;
s4, hatching fertilized eggs: and putting the collected floating fertilized eggs into an incubation barrel for incubation, adding seawater containing guanidine hydrochloride buffer solution into the incubation barrel, sterilizing and removing the membrane, collecting and transferring the fertilized eggs into a culture pond for culture when the hybridized larvae are incubated out of the membrane, and obtaining the golden pompano larvae of the hybridized variety.
Example 4
A cross breeding method of golden pomfret comprises the following steps: the method comprises the following steps:
s1, parent selection and cultivation: selecting trachinotus ovatus fry and trachinotus elongatus fry to be placed into a culture pond, dividing the culture pond into a still water area of a flowing water area by using a movable partition plate, controlling the water temperature of the flowing water area to be 13 ℃, controlling the flow rate to be 0.3m/s, controlling the water temperature of the still water area to be 25 ℃, taking the trachinotus ovatus fry as a male parent, taking the trachinotus elongatus fry as a female parent and taking the ratio of the male parent to the female parent to be 3:1, and transferring the trachinotus ovatus elongatus fry placed into the flowing water area for culture for 15 days to the still water area for static;
s2, artificial induced spawning: artificial induced spawning is carried out on males of the trachinotus ovatus and females of the trachinotus elongatus with well developed gonads, the artificial induced spawning is placed in a running water area for culture, the flow rate is controlled to be 0.9m/s, the induced spawning agents of the male parent of the trachinotus ovatus are luteinizing hormone releasing hormone 26 mu g/kg and diospyrone 3mg/kg, the induced spawning agents of the female parent of the trachinotus elongatus are luteinizing hormone releasing hormone 14 mu g/kg, hormone agonists RH-A3mg/kg and guanethidine 5 mg/kg;
s3, artificial fertilization: after 48h of induced spawning, taking out the semen of the male trachinotus ovatus and the eggs of the female trachinotus ovatus macrobrachi, putting the sperms and the eggs into a glass cup, adding 1 time of seawater, stirring the mixture for 600s by using a glass rod to mix the sperms and the eggs evenly, wherein the volume ratio of the sperms to the eggs is 30:1, and standing the mixture for 10min after artificial fertilization;
s4, hatching fertilized eggs: and putting the collected floating fertilized eggs into an incubation barrel for incubation, adding seawater containing guanidine hydrochloride buffer solution into the incubation barrel, sterilizing and removing the membrane, collecting and transferring the fertilized eggs into a culture pond for culture when the hybridized larvae are incubated out of the membrane, and obtaining the golden pompano larvae of the hybridized variety.
Example 5
A cross breeding method of golden pomfret comprises the following steps: the method comprises the following steps:
s1, parent selection and cultivation: placing the trachinotus ovatus fry and the trachinotus elongatus fry into a culture pond, dividing the culture pond into a still water area of a flowing water area by using a movable partition plate, controlling the water temperature of the flowing water area to be 15 ℃, controlling the flow rate to be 1.0m/s, controlling the water temperature of the still water area to be 30 ℃, taking the trachinotus ovatus fry as a male parent, taking the trachinotus elongatus fry as a female parent and taking the ratio of the male parent to the female parent to be 7:1, and transferring the trachinotus ovatus elongatus fry placed into the flowing water area for 25 days to still water area for static culture;
s2, artificial induced spawning: artificial induced spawning is carried out on males of the trachinotus ovatus and females of the trachinotus elongatus with well developed gonads, the artificial induced spawning is placed in a running water area for culture, the flow rate is controlled to be 1.5m/s, the induced spawning agents of the male parent of the trachinotus ovatus are luteinizing hormone releasing hormone 26 mu g/kg and diospyrone 3mg/kg, the induced spawning agents of the female parent of the trachinotus elongatus are luteinizing hormone releasing hormone 14 mu g/kg, hormone agonists RH-A3mg/kg and guanethidine 5 mg/kg;
s3, artificial fertilization: taking out semen of male trachinotus ovatus and eggs of female trachinotus ovatus after 50h of induced spawning, putting into a glass cup, adding 3 times of seawater, stirring for 100s with a glass rod to mix semen and eggs uniformly, wherein the volume ratio of the semen to the eggs is 50:1, performing artificial fertilization, and standing for 20 min;
s4, hatching fertilized eggs: and putting the collected floating fertilized eggs into an incubation barrel for incubation, adding seawater containing guanidine hydrochloride buffer solution into the incubation barrel, sterilizing and removing the membrane, collecting and transferring the fertilized eggs into a culture pond for culture when the hybridized larvae are incubated out of the membrane, and obtaining the golden pompano larvae of the hybridized variety.
Example 6
The difference between this example and example 3 is that a cross breeding method for golden pomfret: the ratio of the male parent to the female parent in the culture pond in the step S1 is 10: 1.
Example 7
The difference between this example and example 3 is that a cross breeding method for golden pomfret: the temperature of the seawater containing the guanidine hydrochloride buffer solution in the step S4 is 28 ℃.
Example 8
The difference between this example and example 3 is that a cross breeding method for golden pomfret: the volume ratio of the guanidine hydrochloride buffer solution to the seawater in the step S4 is 0.2: 70.
Comparative example 1
The difference between the comparative example and the example 3 is that a cross breeding method for golden pomfret: in the step S2, the oxytocin for the male parent of the trachinotus ovatus is 36 mu g/kg of luteinizing hormone releasing hormone and 10mg/kg of diosdone.
Comparative example 2
The difference between the comparative example and the example 3 is that a cross breeding method for golden pomfret: in the step S2, the oxytocic for the female parent of the trachinotus ovatus is 10 mug/kg of luteinizing hormone releasing hormone, RH-A8mg/kg of hormone agonist and 2mg/kg of guanethidine.
Comparative example 3
The difference between the comparative example and the example 3 is that a cross breeding method for golden pomfret: in the step S3, the volume ratio of the semen to the ovum is 20: 1.
First, cross breeding effect
The cross breeding methods of examples 1 to 8 and comparative examples 1 to 3 were compared after breeding for 3 months, and the fertility rate, hatchability, fry survival rate were tested, respectively, with the following test results:
from the above results, the cross breeding method performed in examples 1 to 8 of the present invention, compared with comparative examples 1 to 2, is characterized in that the spawning induction agent ratio is reasonably adjusted for different pompano genera, and the spawning induction agent is respectively injected into the male parent and the female parent, thereby facilitating gonad development of fish bodies, promoting spermiation and ovulation, and improving the quality of sperms and ova; compared with the comparative example 3, the proportion of the sperms of the semen is scientifically adjusted during artificial fertilization, so that the combination of the sperms and the sperms can be better promoted, and the fertilization rate is improved; compared with the embodiments 1 to 5 and 6, the culture proportion of the male parent and the female parent is adjusted, so that the fish body is stimulated, compared with the embodiments 7 and 8, the temperature of seawater is prepared during hatching, the hatching of fertilized eggs is facilitated, and meanwhile, the guanidine hydrochloride buffer solution is added, so that the demolding of the fertilized eggs is facilitated, and the hatching rate is improved; compared with the embodiments 4 and 5, the embodiments 1 to 3 can perfectly promote the growth and the propagation of the fish body by combining methods such as breeding, fertilization and the like with the oxytocic in a reasonable proportion, and improve the fry survival rate of the cross breeding of the golden pompano.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (8)
1. A cross breeding method of golden pomfret is characterized in that: the method comprises the following steps:
s1, parent selection and cultivation: placing the trachinotus ovatus fry and the trachinotus elongatus fry into a culture pond, dividing the culture pond into a still water area of a flowing water area by using a movable partition plate, controlling the water temperature of the flowing water area to be 13-15 ℃, controlling the flow rate to be 0.3-1.0 m/s, controlling the water temperature of the still water area to be 25-30 ℃, taking the trachinotus ovatus fry as a male parent and the trachinotus elongatus fry as a female parent, and transferring the trachinotus ovatus elongatus fry placed into the flowing water area for culture for 15-25 days to the still water area for static culture;
s2, artificial induced spawning: artificial spawning induction is carried out on males of the trachinotus ovatus and females of the trachinotus elongatus with well developed gonads, the artificial spawning induction is carried out in a running water area for culture, the flow rate is controlled to be 0.9-1.5 m/s, the spawning induction agent of the male parent of the trachinotus ovatus is 20-30 mu g/kg of luteinizing hormone releasing hormone and 2-5 mg/kg of deovone, the spawning induction agent of the female parent of the trachinotus elongatus is 12-20 mu g/kg of luteinizing hormone releasing hormone, hormone agonist RH-A2-5 mg/kg and guanethidine 3-8 mg/kg;
s3, artificial fertilization: after 48-50 h of induced spawning, taking out the semen of the male trachinotus ovatus and the eggs of the female trachinotus ovatus, putting the sperms and the eggs into a glass cup, adding 1-3 times of seawater, stirring the mixture for 60-100 s by using a glass rod to ensure that the sperms and the eggs are mixed uniformly, wherein the volume ratio of the sperms to the eggs is 30-50: 1, and standing the mixture for 10-20 min after artificial fertilization;
s4, hatching fertilized eggs: and putting the collected floating fertilized eggs into an incubation barrel for incubation, adding seawater containing guanidine hydrochloride buffer solution into the incubation barrel, sterilizing and demolding, collecting and transferring the fertilized eggs into a culture pond for culture when the hybridized larvae hatch out of the membrane, and obtaining the golden pomfret larvae of the hybridized variety.
2. The cross breeding method of golden pomfret as claimed in claim 1, which is characterized in that: the ratio of the male parent to the female parent in the culture pond in the step S1 is 3-7: 1.
3. The cross breeding method of golden pomfret as claimed in claim 1, which is characterized in that: in the step S1, the water temperature in the flowing water area is 14 ℃, the flow rate is 0.7m/S, and the water temperature in the still water area is 28 ℃.
4. The cross breeding method of golden pomfret as claimed in claim 1, which is characterized in that: in the step S2, the oxytocin for the male parent of the trachinotus ovatus is 26 mu g/kg of luteinizing hormone releasing hormone and 3mg/kg of diospyrone.
5. The cross breeding method of golden pomfret as claimed in claim 1, which is characterized in that: in the step S2, the oxytocic for the female parent of the trachinotus ovatus is 14 mu g/kg of luteinizing hormone releasing hormone, RH-A3mg/kg of hormone agonist and 5mg/kg of guanethidine.
6. The cross breeding method of golden pomfret as claimed in claim 1, which is characterized in that: and the volume ratio of the semen to the ovum in the step S3 is 40: 1.
7. The cross breeding method of golden pomfret as claimed in claim 1, which is characterized in that: the temperature of the seawater containing the guanidine hydrochloride buffer solution in the step S4 is 20-25 ℃.
8. The cross breeding method of golden pomfret as claimed in claim 1, which is characterized in that: the volume ratio of the guanidine hydrochloride buffer solution to the seawater in the step S4 is 0.3-0.8: 20-50.
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